Morphological and genetic diversity of the larch populations

on the Kuril Island

I.Yu. Adrianova

Group of Plant Molecular Genetics

Institute of Biology and Soil Sciences, FEB RAS

To study the influence of island isolation on the morphological and genetic diversity of the populations of Larix kamtchatica (Rupr.) Carr. from Iturup Island

The purpose of this research:

Slide 2

The schemes of location of the larch populations studied

- place of the sample collecting Slide 3

•the length of the seed wing

The morphological traits of cones and seeds used in this work

•the length and width of the cone

•the ratio of length to width of the cone

•the deflection angle of scales in the cone

The quantitative trait:

The qualitative trait:

•the shape of the scales •the character of scale edge

•the degree of deflection of the scale edge

• the number of scales in the cone

•the length and width of the seed

Slide 4

Level of variability CV, % Level of variability CV,%

Very low < 7 Higher 21-30

Low 8-12 High 31-40

Medium 13-20 Very high >40

Levels of variability of morphological traits of wood species worked out by S.A. Mamaev

CV – coefficient of variation

Individual variability of all morphological traits ranged from 8 to 21 %.

AMOVA analysis based on all morphological traits doesn’t distinguish these populations (p = 0.204).

Slide 5

Kas_1

Kas_2

Kas_3

Kas_4

Kas_5

Kas_6

Kas_ 7

Kas_8

Khv _1Khv _2

Khv _3

Khv _4

Khv _5

Khv _6 Khv _7

Khv _8Khv _9

Khv _10

Khv _11

Khv _12Khv _13

Khv _14

Khv _15

Khv _16

Khv _17

Khv _18

-7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6

Factor 1: 33 .45%

-5

-4

-3

-2

-1

0

1

2

3

4

5

Fac

tor

2: 2

0.8

6%

PCA ordination of the larch individuals using all morphological traits

Slide 6

Kas- Kasatka

Khv - Khvoinyi

The dendrogram of relationship between trees from two larch populations of Iturup Island based on all

morphological traits of cones and seeds

Ward`s methodEuclidean distances

Kh

v_8

Kh

v_6

Kas

_6

Kh

v_16

Kh

v_13

Kh

v_12

Kh

v_9

Kh

v_7

Kh

v_17

Kas

_2

Kh

v_11

Kh

v_18

Kh

v_5

Kh

v_4

Kh

v_1

Kas

_8

Kas

_7

Kh

v_3

Kas

_5

Kas

_4

Kh

v_10

Kh

v_2

Kh

v_15

Kh

v_14

Kas

_3

Kas

_1

0

100

200

300

400

500

600

Lin

kag

e D

ista

nce

Kas- Kasatka

Khv– Khvoinyi

Slide 7

Slide 8

Sample A P95,% P99,% He I h

Kasatka 1.39 38.9 38.9 0.1339 0.1270 0.19

Khvoinyi 1.55 43.3 50.5 0.1494 0.1507 0.23

The genetic estimations of our populations

A - Observed number of alleles; P95 - % polymorphic loci (95% criterion) ; P99 - % polymorphicloci (99% criterion); He – average expected heterozygosity; I -Shannon's Information index [Lewontin (1972)]; h = Nei's (1973) gene diversity

Larix sibirica Ldb. - A = 1.54; P95 = 45%; He = 0.1698

Larix gmelinii (Rupr.) Rupr. - A= 1.32; P95 = 32%; He = 0.1373

X-sq : 327.6221df : 638Overall: p = 1.0000

Exact tests for population differentiation (Raymond and Rousset, 1995)

Slide 9

UPGMA unrooting tree of the individuals from two larch populations based on Nei’s (1978) genetic distances

Kas- Kasatka

Khv– Khvoinyi

Nm = 5.1

Conclusions:

The island isolation have not caused a decrease of morphological and genetic diversity of the larch populations from Iturup Island

Slide 10

Thank you for your attention !