INVESTIGATION OF PHYTOCHEMICALS, ANTIOXIDANT ACTIVITIES AND ANTIMICROBIAL

ACTIVITIES OF MORINDA CITRIFOLIA LINN

S.ANBAZAHAN II M.Sc BIOTECHNOLOGY

RATHNAVEL SUBRAMANIAM COLLEGE OF ARTS AND SCIENCE COIMBATORE-641402

INTRODUCTION

Kingdom: Plantae

Order : Gentianales

Family : Rubiacae

Genus : Morinda

Species : Morinda citrifoliaMorinda citrifolia L. also known as Noni or Indian 

mulberry is a small evergreen tree. It is one of the most important traditional Polynesian 

medicinal plants.

PHYTOCHEMICAL SCREENING

India is known for its rich diversity of medicinal

plants.

The bioactive compounds and medicinal power

mainly depends on phytochemical constituents that have

great pharmacological significance.

Phytochemicals are classified into two major groups

namely primary constituents and secondary constituents

MORINDACITRIFOLIA LINN AS ANTIOXIDANTS

Plants are the potential source of natural

antioxidants.

Morinda citrifolia L. its wide range of antioxidants

can be a major source of natural or phytochemical

antioxidants

Beta-carotene, ascorbic acid and alpha tocopherol are

the widely used antioxidants. 

ANTIMICROBIAL ACTIVITIES OF MORINDA CITRIFOLIA LINN

Antibacterial activities Acubin, L-asperuloside, and

alizarin in the Nona fruit compounds are antibacterial

agents.

These compounds have been shown to fight against

infectious bacteria strains such as Pseudomonas

aeruginosa, Proteus morgaii, Staphylococcus aureus,

Baciillis subtilis, Escherichia coli, Salmonella, and

Shigela.

MEDICINAL USES OF MORINDA CITRIFOLIA

Antioxidant / Anticancer Nitric Oxide Scavenging Activity Herbal Hepatotoxicity Antispasmodic / Vasodilator Activities Analgesic Activities Wound Healing / Antioxidant Antiviral / Cytotoxicity Apoptosis-Inducing Effects Antidyslipidemic Activities

AIM AND OBJECTIVESAIM:

The study was design to investigate the Phytochemicals

Antioxidants and Antimicrobial activities from different

parts of Morinda citrifolia plant.

OBJECTIVES: Phytochemical Screening Antioxidant activities Antimicrobial activities

MATERIALS AND METHODSPLANT COLLECTION: Fresh leaves, bark and fruits were collected from Thanjavur and different locations of Pudukkottai , Tamilnadu, India.

PREPARATION OF EXTRACT: Aqueous extract, Ethanol extract and Methanol extract.

RESEARCH METHODS: Safowara (1993), trease and Evans (1989) and Harborne (1973),FRAP and Disc Diffusion methods.

RESULTS OF MORINDA CITRIFOLIA PHYTOCHEMICAL TEST

FEHLING’S TEST TERPENOIDS

RESULTS OF MORINDA CITRIFOLIA PHYTOCHEMICAL TEST

TANNINS PHLOBATANNINS

RESULTS OF MORINDA CITRIFOLIA PHYTOCHEMICAL TEST

ANTHRAQUINONES FLAVONOIDS

RESULTS OF MORINDA CITRIFOLIA PHYTOCHEMICAL TEST

SAPONINS CARDIAC GLYCOSIDES

RESULTS OF ANTIOXIDANT ACTIVITIES BY

FRAP ASSAY

SL.NO NAME OF THE SAMPLE

FRAP CONTENT IN (g/1OOg)

1 LEAF 1.2452 BARK 0.2023 FRUIT 1.074

LEAF BARK FRUIT0

0.2

0.4

0.6

0.8

1

1.2

1.41.24499999999999

0.202

1.074

Series1

ANTIMICROBIAL ACTIVITIES OF MORINDA CITRIFOLIA LEAF, BARK

AND FRUIT EXTRACT

Sl.No Name of the medium

Name of the organism

Zone of inhibition(cm)

Leaf Bark Fruit1 Nutrient agar Aspergillus niger 1.0 cm 1.5 cm 1.0 cm2 Nutrient agar Aspergillus oryza - 1.0 cm -3 Nutrient agar Salmonella 1.7 cm - -4 Rose Bengal agar Aspergillus niger - - -

5 Rose Bengal agar Aspergillus oryza - 0.7 cm -

6 Rose Bengal agar Salmonella - - -

CONCLUSION

The present investigation on analysis of

Phytochemicals, Antioxidant and Antimicrobial

Activities of Morinda citrifolia were carried out at the

Food Quality and Testing Laboratory, IICPT, Thanjavur.

In this study phytochemical screening, the

antioxidant activities by FRAP method was carried out.

The antimicrobial activities of the plant extract were

also carried out.

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