natiinfvg: nipt for traceable and computerized ... · laboratory traceability speaker: dott. diego...
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NATIinFVG: NIPT FOR TRACEABLE AND COMPUTERIZED ANEUPLOIDIES IN FRIULI VENEZIA GIULIA
LABORATORY TRACEABILITY
Speaker: Dott. Diego Boscarino (AB ANALITICA SRL)
Final Event, Trieste 17th October 2019
NATIinFVG Final Event, Trieste 17th October 2019
PRESENTATION OVERVIEW
COMPANY PROFILE
AB ANALITICA IN THE NATIinFVG PROJECT
TRACEABILITY
AUTOMATION
AB ANALITICA Advance Biomedicine
«We design and produce solutions
for better diagnostics»
❖ Strong experience in the production of
diagnostic kits and reagents
❖ Since 30 years scientists develop
innovative test in the diagnostic field
❖ Provider of the best assistance,
technical support and customers
service
❖ Eco sustainable company
WHO IS AB ANALITICA
La nostra crescitaAB ANALITICA is present in over 40 Countries
with its trusted distributor network
AB ANALITICA is fully
committed in matching
both talent and skills
of its researchers
R&D Team
Engineering Team
Technical assistance &
Customer support
Production Team
DIAGNOSTIC APPLICATION FIELDS
Infectivology Human Papillomavirus STI Hepatitis C virus
Oncohematology Thrombophilia HLA
REVERSE LINE BLOT
NEXT GENERATION SEQUENCING
REAL TIME PCR
Technologies
SMART WORKFLOW
EFFICIENT AUTOMATION
Panel of RT-PCR kits with samethermal profile, mastermixready to use, IC anddUTP/UNG inside
Automated solution sample to results. Extraction, PCR set-up, amplification and interpretation
INTERNAL PRODUCTION
SOFTWARE DEVELOPMENTIngeneering team fordevelopement ofinterpretation software andautomation software
Certified UNI EN ISO 9001and UNI EN ISO 13485 for allproducts potfolio
HIGHT SPECIFICITY and PERFORMANCE
30 years of experience indevelop of diagnostic kits
High professional andqualified production teamfor all AB ANALITICA kits
QUALITY CERTIFIED
customer planning input
developing
output
check
Final product
validation
transfer
Research & Development Workflow
NATIinFVG Final Event, Trieste 17th October 2019
AB ANALITICA IN THE NATIinFVG PROJECT
KNOWLEDGE ACQUISITION AND CONCEPTUAL DEFINITION
• Analysis of transfer steps in the whole process (sample and related information)
• Storage requirements (short-term and long-term)
• Nucleic acid extraction requirements for NGS downstream analysis, traceability and automation
• Library preparation requirements for traceability and automation
ACHIEVEMENTS
• Project biobank
• Traceability system
• System for automatic library preparation
NATIinFVG Final Event, Trieste 17th October 2019
TRACEABILITY: BASE PLATFORM
AB
PRINTERAB SCAN ARRAY
AB SCAN
SINGLE BioBanker
NATIinFVG Final Event, Trieste 17th October 2019
Ultra congelatori e/o Freezer
Rack per stoccaggio provette
Stampanti etichette manuale e
automatizzate
Capper/Decapper
per tappi a vite e
pressioneSistema
riconoscimento
BIOMETRICO
Sistema
Monitoraggio
Temperatura
AB ANALITICA GENEQUALITY
X 120
con sistemi di lettura barcode
1D e scansione 2D
TRACEABILITY: BASE PLATFORM
NATIinFVG Final Event, Trieste 17th October 2019
TRACEABILITY: ANALYSIS
Visual workflow
Data flow
Flow chart
NATIinFVG Final Event, Trieste 17th October 2019
TRACEABILITY: IMPLEMENTATION
Exploitation of existing functionalities
• 2 progress status fields (DNA extraction and Library Preparation) + primary code tracking field
• DNA extraction phase: barcode check of primary code on printed label physically propagated
through all the steps
• Library preparation phase: plate well coordinates replicated through the aliquote functionality
Custom functionalities (designed for more general application)
• Valorization of the tracking field at sample creation by linking to existing fields (software)
• ID Code generator (software)
• Plate pipetting masks with barcode identification (software + hardware)
• Traceability of QIASymphony output rack to input tube carrier (software + hardware)
NATIinFVG Final Event, Trieste 17th October 2019
AUTOMATION: DECK LAYOUT
NATIinFVG Final Event, Trieste 17th October 2019
AUTOMATION: PROGRAMMING
