new, modern textiles as wrapping material for...
TRANSCRIPT
![Page 1: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/1.jpg)
NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR
STERILIZATION
VLATKA TURCIC, M.Sc.CROATIA
![Page 2: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/2.jpg)
Greetings from Croatia
CROATIAVlatka Turcic
![Page 3: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/3.jpg)
Vlatka TurcicCROATIA
Beti Rogina‐Car, Ph.D.Vlatka Turcic, M. Sc.Ana Budimir, Ph. D.Drago Katovic, Ph.D.
![Page 4: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/4.jpg)
Beti Rogina-Car, Ph.D.Vlatka Turcic, M. Sc.Ana Budimir, Ph. D.Drago Katovic, Ph.D.
This scientific research was conducted for the dissertation of candidate Beti Rogina – Car in cooperation with:
• Faculty of Textile Technology Zagreb • University Clinical Hospital Centre Zagreb,
Croatia
CROATIAVlatka Turcic
![Page 5: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/5.jpg)
The Use of Textiles in Medicine
CROATIAVlatka Turcic
![Page 6: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/6.jpg)
The Use of Textiles in
Sterilization
CROATIAVlatka Turcic
![Page 7: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/7.jpg)
• undeclared quality• microbial barrier - questionableCotton
No data about a range of other characteristics of cotton fabrics required for ensuring microbial barrier
CROATIAVlatka Turcic
![Page 8: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/8.jpg)
Under appropriate conditions of humidity and temperature, most medical textiles MADE OF natural fibers present
an excellent basis for bacterial and fungal growth
CROATIAVlatka Turcic
![Page 9: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/9.jpg)
The requirements that must be met by the packaging materials together with the rules of handling are determined by
HRN EN ISO 11607-1:2010 [28].
CROATIA
![Page 10: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/10.jpg)
CROATIAVlatka Turcic
![Page 11: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/11.jpg)
WRAPPING MATERIAL
easy packing
effective sterilization
reliable storage
safe handling
maintains sterility
aseptic opening
CROATIAVlatka Turcic
![Page 12: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/12.jpg)
microbial barrier
Requirements to provide microbial barrier
Enable sterilizationProvide a barrier to microorganismsEnable maintaining sterility
Sterilization medium
Microorganisms
TextileBARRIER
CROATIAVlatka Turcic
![Page 13: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/13.jpg)
Dry conditionHRN EN ISO 22612:2008
Wet conditionHRN EN ISO 22610:2008
TEST OF RESISTANCE TO PENETRATION OF MICROORGANISMS IN DRY AND WET CONDITION FOR SURGICAL GOWNS
CROATIAVlatka Turcic
![Page 14: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/14.jpg)
Three types of textiles were selected for testing:
• Cotton/PES 50%/50%• Tencel ® 100%
(lyocell fibres with trade name)• Three-layer textile laminate PES/PU/PES
(known as operating, OP laminate)
All of declared and standardized quality
New medical textiles
CROATIAVlatka Turcic
![Page 15: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/15.jpg)
Samples Raw material content Weave
Sample I Polyester fiber/cotton50%/50%
Cloth
Sample II Tencel®Twill 2/1
Sample III Three-layer textile laminate PES/PU/PES:
1.weft right /right interlock knit,2.PU membrane,3.Warp’s charmeuse knit (knitted back)
CHARACTERISTICS OF THE APPLIED TEXTILES
CROATIAVlatka Turcic
![Page 16: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/16.jpg)
Differences in the structure and form
Cotton/PES Tencel ® OP Laminate
®
CROATIAVlatka Turcic
![Page 17: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/17.jpg)
All samples were tested after multiple washing and sterilization:
For mechanical influences (strength and elongation)
For air permeability
For permeability of microorganisms in dry conditions of extreme contamination
For permeability of microorganisms afterstorage in controlled storage conditions
CROATIAVlatka Turcic
![Page 18: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/18.jpg)
Each sample was tested on a dynamometer for strength and elongationbefore washing and sterilizationafter the 1th, 10th, 20th, 30th and 50th
washing and sterilization
Strength and elongation
CROATIAVlatka Turcic
![Page 19: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/19.jpg)
Mechanical influences (strength and elongation)
0
100
200
300
400
500
600
700
800
900
0W+S 1W+S 10W+S 20W+S 30W+S 50W+S
PES/pamuk 50%/50%
Broj procesa pranja i sterilizacije
Fp (N
)
0,00
5,00
10,00
15,00
20,00
25,00% F(N)
Є (%)
0100200300400500600700800900
1000
0W+S 1W+S 10W+S 20W+S 30W+S 50W+S
Tencel®
Broj procesa pranja i sterilizacije
Fp (N
)
0,00
5,00
10,00
15,00
20,00
25,00%
F(N)Є (%)
0
100
200
300
400
500
600
700
0W+S 1W+S 10W+S 20W+S 30W+S 50W+S
Troslojni tekstilni laminat PES/PU/PES
Broj procesa pranja i sterilizacije
Fp (N
)
0,00
10,00
20,00
30,00
40,00
50,00
60,00
70,00
80,00
90,00%
Fp(N)Є (%)
RESULTS 1 ,10, 20, 30, 50 W & S
CROATIAVlatka Turcic
![Page 20: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/20.jpg)
Textile material shrinks during washing and sterilization whichresults that the density of the fabric increases while the penetration of air and microorganisms decreases
Air and Microorganisms Permeability
CROATIAVlatka Turcic
![Page 21: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/21.jpg)
RESULTS
Changes in density of PES/cotton
0W+S 10W+S 20W+S 30W+S 50W+S
CROATIA
![Page 22: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/22.jpg)
RESULTSChanges in density of Tencel®
0W+S 10W+S 20W+S 30W+S 50W+S
CROATIA
![Page 23: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/23.jpg)
RESULTSChanges in density of three-layer textile laminate PES/PU/PES
0W+S 10W+S 20W+S 30W+S 50W+S
CROATIA
![Page 24: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/24.jpg)
AIR PERMEABILITY
RESULTS
CROATIA
![Page 25: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/25.jpg)
• Three-layer textile laminate is completely air impermeable due to its polyurethane membrane. However, it should be noted it is permeable to a sterilization medium which gives it a basic criteria forsterilization
• In the PES/cotton and Tencel® blend, it is visible that air permeabilitycontinuously decreases after washing and sterilization which can be explainedwith the fact that the textile shrinks during washing and sterilization
RESULTS
Airpermeability
CROATIAVlatka Turcic
![Page 26: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/26.jpg)
In dry conditions of extreme contamination
After storage in controlledstorage conditions
PERMEABILITY OF MICROORGANISMS
CROATIAVlatka Turcic
![Page 27: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/27.jpg)
PES/Coton 50%/50%PES/Coton 50%/50%
1W+S10W+S20W+S30W+S50W+S
Process of washing and sterilizationProcess of washing and sterilization
Three-layer textile laminatePES/PU/PES
Three-layer textile laminatePES/PU/PESTencel®Tencel®
Storage 1, 2 and 3 monthsStorage 1, 2 and 3 monthsPermeabilitytest
Permeabilitytest
3 x 30 pcs
3 x 6 pcs
Ʃ 360 pcs
3 x 30 pcs
3 x 30 pcs3 x 30 pcs
Ʃ 90 pcs
3 x 6 pcs
3 x 6 pcs
3 x 6 pcs
3 x 6 pcs
RESEARCH PLAN
CROATIAVlatka Turcic
![Page 28: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/28.jpg)
Constructed and executed device for testing microbial barrierefficiency of medical textiles
PERMEABILITY OF MICROORGANISMS
CROATIAVlatka Turcic
![Page 29: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/29.jpg)
134 ⁰C/5 minSTERILIZATION
CROATIAVlatka Turcic
![Page 30: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/30.jpg)
Bacterial endospores Geobacillus Stearotermophilus 10⁵and Bacillus Atrophaeus 10⁶ were used
PERMEABILITY OF MICROORGANISMS IN DRY CONDITIONS OF EXTREME CONTAMINATION
CROATIAVlatka Turcic
![Page 31: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/31.jpg)
PERMEABILITY OF MICROORGANISMS
INCUBATION48 h + 35°C
INCUBATION 24 h
CROATIAVlatka Turcic
![Page 32: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/32.jpg)
PERMEABILITY OF MICROORGANISMS
Process of collecting prints from the test samples, using CT3P agar plates
Lower side Upper side
CROATIAVlatka Turcic
![Page 33: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/33.jpg)
PERMEABILITY OF MICROORGANISMS
PES/cottonFRONT
RESULTS
BACK
CROATIAVlatka Turcic
![Page 34: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/34.jpg)
PERMEABILITY OF MICROORGANISMS
Tencel®FRONT BACK
RESULTS
CROATIAVlatka Turcic
![Page 35: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/35.jpg)
Three-layer textile laminatePES/PU/PES
BACKFRONT
RESULTSPERMEABILITY OF MICROORGANISMS
CROATIAVlatka Turcic
![Page 36: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/36.jpg)
SamplesNo. of washing and sterilization
processses
CFU on thefront of the
taxtile
CFU on the backof
the taxtile
Front - backrationCFU
Samples I
PES/cotton50%/50%
1 W + S 356 11 32:1
10 W + S 275 14 20:1
20 W + S 318 9 35:1
30 W + S 286 7 41:1
50 W ‘S 396 2 198:1
Samples II
100% Tencel®
1 W + S 419 7 60:1
10 W + S 359 8 45:1
20 W + S 294 2 147:1
30 W + S 182 3 60:1
50 W + S 341 2 170:1
Samples III
Three-layer textilelaminatePES/PU/PES
1 W + S 155 0 -
10 W + S 167 0 -
20 W + S 175 0 -
30 W + S 132 0 -
50 W + S 464 0 -
RESULTSPERMEABILITY OF MICROORGANISMS
CROATIAVlatka Turcic
![Page 37: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/37.jpg)
RESULTSPERMEABILITY OF MICROORGANISMS
Regression analysis of how washing and sterilization affects microbial barrier permeability in medical textiles
CROATIAVlatka Turcic
![Page 38: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/38.jpg)
PERMEABILITY OF MICROORGANISMS IN CONTROLLED STORAGE CONDITIONS
CROATIAVlatka Turcic
![Page 39: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/39.jpg)
Microclimate conditions
Temperature: 15 – 30 °CRelative humidity : 30 – 60%
PERMEABILITY OF MICROORGANISMS IN CONTROLLED STORAGE CONDITIONS
The material to be stored on shelves must be located:
• 25 cm from the floor, • 45 cm from the ceiling • 5 cm from the walls
CROATIAVlatka Turcic
![Page 40: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/40.jpg)
Brain-Heart liquidbroth
INCUBATION48 h + 35°C
PERMEABILITY OF MICROORGANISMS IN CONTROLLED STORAGE CONDITIONS
CLARITY OF THE BROTH
Absorbing paper 1cm²
CROATIAVlatka Turcic
![Page 41: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/41.jpg)
Permeability of microorganisms in controlled storage conditions
Brain-Heart liquidbroth
0,5 ml
INCUBATION48 h + 35°C
COUNTING OF BACTERIAL COLONIES
BLOOD AGARAbsorbing paper 1cm²
CROATIAVlatka Turcic
![Page 42: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/42.jpg)
Samples Number of washing andsterilization procedures
Storage time
1 month 2 months 3 months
Samples I
PES/cotton50%/50%
10 washings & sterilizations NMG NMG NGM
20 washings & sterilizations NMG NMG NMG
30 washings & sterilizations NMG NMG NMG
50 washings & sterilizations NMG NMG NMG
Samples II
100% Tencel®
10 washings & sterilizations NMG NMG NMG
20 washings & sterilizations NMG NMG NMG
30 washings & sterilizations NMG NMG NMG
50 washings & sterilizations NMG NMG NMG
Samples III
Three-layer textile laminatePES/PU/PES
10 washings & sterilizations NMG NMG NMG
20 washings & sterilizations NMG NMG NMG
30 washings & sterilizations NMG NMG NMG
50 washings & sterilizations NMG NMG NMG
RESULTSPERMEABILITY OF MICROORGANISMS IN CONTROLLED STORAGE CONDITIONS
CROATIAVlatka Turcic
![Page 43: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/43.jpg)
3 TYPES OF TEXTILES (cotton/PES, Tencel®, PES/PU/PES)
Strenght Tencel ® Cotton/PES
Elongation PES/PU/PES Tencel ®
Air permeability Tencel ® PES/PU/PES
Permeability formicroorganisms Cotton/PES PES/PU/PES
Permeability for microorganisms after storage
CROATIAVlatka Turcic
![Page 44: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/44.jpg)
It is evident that Tencel is the most resistant one to tearing, while OP laminate recorded the highest level of elongation.
The most permeable to air is Tencel, while the three-layer laminate is completely impermeable to air and microorganisms. The worstcharacteristics showed a cotton / PES.
Nevertheless, none of the tested textile material is not permeable formicroorganisms after storage of three months.
Electronic microscope recorded damages on membranes of the OP laminate after 50 washings and sterilization, which were not present after 30 procedures.
BETORE THE CONCLUSION
CROATIAVlatka Turcic
![Page 45: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/45.jpg)
The tested cellulosic textiles and three-layer textile laminate, even in one layer can be used as wrapping material for sterilization under conditions described in the research and provide a microbial barrier after sterilization.
Microbial barrier is safe against contamination during the test period of 3 months and after 50 washing and 50 sterilization procedures.
C O N C L U S I O N
CROATIAVlatka Turcic
![Page 46: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/46.jpg)
Thank you for your attention
Have a nice rest of the day
CROATIAVlatka Turcic
![Page 47: NEW, MODERN TEXTILES AS WRAPPING MATERIAL FOR …bib.irb.hr/datoteka/768309.FONDO_CONG_BQUILLA_2015.pdf · CROATIA VLATKA TURCIC, M.Sc. Greetings from Croatia CROATIA Vlatka Turcic](https://reader033.vdocument.in/reader033/viewer/2022051605/600b00f75c09f040214c362e/html5/thumbnails/47.jpg)
Vlatka Turcic
CROATIA