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Nordic New! KAPA Hyper Prep Kits optimized for FFPE NGS New tools for your WB Easy and sensitive - test it! Superior SDS-PAGE! Excellent resolution and no gel tear! Reagent for research or clinical diagnostics - we can help you! Tired of pipetting by hand? Automate with Cybio Meet us at CPH LabMed 7-9/10 Lab14/Oslo 28-30/10 Kem-En-Tec Nordic A/S Kuldyssen 10 DK-2630 Taastrup Phone +45 3927 1777 [email protected] www.kem-en-tec-nordic.com All prices are valid until 31. December 2014. Local currency exclusive VAT and Ex Works. New! Sense Microplate Reader - it has never been easier! NEWSLETTER 2. 2014 K em -E n -T ec N ordic COMPETENT BIOSCIENCE SINCE 1987

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  • NordicNew! KAPA Hyper Prep Kits optimized for FFPE NGS

    New tools for your WBEasy and sensitive - test it!

    Superior SDS-PAGE!Excellent resolution and no gel tear!

    Reagent for research or clinical diagnostics- we can help you!

    Tired of pipetting by hand?Automate with Cybio

    Meet us atCPH LabMed 7-9/10Lab14/Oslo 28-30/10

    Kem-En-Tec Nordic A/S Kuldyssen 10DK-2630 TaastrupPhone +45 3927 [email protected] www.kem-en-tec-nordic.com

    All prices are valid until 31. December 2014. Local currency exclusive VAT and Ex Works.

    New! Sense Microplate Reader - it has never been easier!

    NEWSLETTEr 2. 2014

    Kem-En-Tec NordicCOMPETENT BiOSCiENCE SiNCE 1987

  • Figure 1: Figure 1: KAPA Hyper Prep Kits convert more input DNA to adapter-ligated library. Libraries were prepared from Covaris-sheared DNA and quantified prior to library amplification with the KAPA Library Quantification Kit. The KAPA Hyper Prep Kits achieved the highest yield of adapter-ligated library across the range of inputs (1 µg, 100 ng or 10 ng), and required fewer cycles of amplification to generate 1 µg of amplified library required for target capture.

    Figure 2: Sequencing metrics FFPE libraries prepared for whole exome sequencing. Libraries were prepared from 100 ng of FFPE DNA, and exome capture was performed with the SeqCap EZ HGSC VCrome Design, roche NimbleGen. Higher library yields enabled fewer cycles of amplification, leading to significantly lower duplication rates and higher target coverage.

    request a trial kit

    Figure 3: The streamlined KAPA Hyper Prep workflow. End repair, A-tailing and ligation are performed in a single tube, without intervening cleanup steps.

    The workflow is ideally suited for robust, high-throughput sample preparation for Illumina sequencing.

    KAPA Hyper Prep Kits

    Higher performance. Even from FFPE.The KAPA Hyper Prep Kit is a versatile, streamlined solution for DNA library preparation for Illumina sequencing. The novel one-tube chemistry, opti-mally formulated and evolved enzymes enable higher yields of adapter- ligated library and lower amplification bias. This translates to higher library diversity, lower duplication rates and more uniform coverage, particularly for FFPE and low-input samples.

    • Robust performance with inputs from 1 ng – 1 µg• Fast, one-tube workflow (2 – 3 hours) with minimal hands-on time• Compatible with whole genome sequencing, targeted sequencing,

    PCR-free workflows, FFPE and low input samples• Contains KAPA HiFi and an optimized Library Amplification Primer Mix

    improve library yields and sequence quality• Higher library yields translate to greater molecular complexity• Fewer cycles of amplification with KAPA HiFi DNA Polymerase results in

    lower duplication rates and improved coverage

    Create high-quality libraries from FFPE samples• Generate high-quality libraries from 250 ng FFPE DNA or less• Significantly lower duplication rates and higher coverage

    Achieve high quality libraries in less than 3 hours• One-tube workflow with minimal cleanup steps

    reduces overall time, and minimizes hands-on time• Sample-to-library in

  • New improved tools for your SDS-PAGE and Western Blots

    RunBlue precast SDS-PAGE gels – Superior resolution and no tear!• 10 x stronger gel, that do not tear!• Colored well separators ease loading of gels• Up to 2 year shelf life• 40 min run time• Gels for both Invitrogen and BioRad cells

    ORDERiNG iNFO: ALL GEL TyPES - SAME PRiCE: 975,- DKK 1165,- SEK 1085,- NOK 130 €

    for a box of 10 gels - regardless of % and number of wells – Contact us for a complete product list and quote for larger volumes.

    instantBlue - The 15 min Coomassie!Ready-to-use - Add to gel – Wait 15 min – No need for destaining - 5 ng sensitivity (BSA)

    ORDERiNG iNFO: 1 LiTRE iNSTANTBLuE CAT # iSB1L

    1430,- DKK 1705,- SEK 1590,- NOK 190 €

    NEW! - Light up your WB markers with NEW AntiBlue AbSave money and improve your WB assay!Easy - With Anti-Blue your blue prestained marker is detected together with your sample proteins – In the same exposure. No need for multiple exposures and overlaying images.

    *Buy before end of October and get 25 % OFF your first purchase (expire october 31st 2014)

    Economical - With AntiBlue Ab you save on your expensive prestained marker - You only need to load 0,5 µl marker. The savings on marker will greatly surpass the cost of Anti-Blue. Anti-Blue can be used in dilution from 1:2000 to 1:10000. AntiBlue has no cross reactivity to proteins and will only bind to the blue stain on prestained markers.

    RunBlue gels

    is now also available

    with NuPAGE identical buffers.

    See it at CPH LabMed Booth: 6

    Product Description List Price introduction PriceCode 25 % OFF*

    ABF0100 Anti-Blue Antibody 100 µl 950,- DKK 712,- DKK 1120,- SEK 840,- SEK 1055,- NOK 791,- NOK 127,- € 95,- €

    ABH0100 HRP labelled Anti-Blue 1195,- DKK 895,- DKK Antibody 100 µl 1405,- SEK 1054,- SEK 1330,- NOK 998,- NOK 160,- € 120,- €

    FREE testpackage available – Try RunBlue gels and InstantBlue stain in your own lab.

    Kem-En-Tec Nordic · [email protected] · www.kem-en-tec-nordic.com 3

  • Capillary electrophoresisFragment Analyzer – the perfect tool for your DNA and rNA analysis

    Molecular biologySee the newest clever gadgets for visualization of DNA

    The world’s most efficient polymerasesExperience the Kapa Biosystems solutions first hand

    Lab instrumentsSee and try the hot new labora-tory instruments from BioSan

    New Tristar² S Multilabel reader with monochromator- great introduction discounts

    ELiSA • Crocodile ELISA mini workstation

    – Run your ELISA for you• Plate washers 8 and 96 channel

    – Also good for Plate coating• ELISA Readers & automation –

    LED based solutions• ELISA Reagents – Ready to use

    and safe

    Electrophoresis and Fractionation• MW protein fractionator Gel-

    Free8100 – An ideal pre MS analysis tool

    • RunBlue SDS-PAGE – Precast gels with superior resolution and so strong that they show no tears!

    • InstantBlue – The 15 min Coomassie – Quick and Easy gel staining

    Copenhagen LabMed October 7-9, 2014Lokomotivværk

    stedet · Booth no 6

    What’s on display?Visit our booth Learn tips and tricks from our product specialists

    Attend a demonstration and have a chance to win prices

    See last page for information regarding address and tickets

    Capillary electrophoresisFragment Analyzer – the perfect tool for your DNA and RNA analysis

    Liquid handlingCyBi®-SELMASemi-automatic 96- or 384-fold pipettor

    Molecular biologySee the newest clever gadgets for visualization of DNA

    The world’s most effi cient polymerases Experience the Kapa Biosystems solutions fi rst hand

    Lab instrumentsSee and try the hot new labora-tory instruments from BioSan

    Diagnostics- and research kits• ELISA• PCR • NGS kits

    Research reagents for• Glycobiology, • Cell biology • Flow cytometry

    Liquid handlingCyBi®-FeliXCompact pipetting platform for fully automated single- to multichannel liquid handling

    New Tristar² S Multilabel reader with monochromator- great introduction discounts

    Multilabelreader Sense: Microplate reader with touchscreen – it has never been easier

    ELISA • Crocodile ELISA mini workstation

    – Run your ELISA for you• Plate washers 8 and 96 channel

    – Also good for Plate coating• ELISA Readers & automation –

    LED based solutions• ELISA Reagents – Ready to use

    and safe

    Electrophoresis and Fractionation• MW protein fractionator Gel-

    Free8100 – An ideal pre MS analysis tool

    • RunBlue SDS-PAGE – Precast gels with superior resolution and so strong that they show no tears!

    • InstantBlue – The 15 min Coomassie – Quick and Easy gel staining

    Copenhagen LabMed October 7-9, 2014

    Most of the news will also be shown at Lab14 in Norway, see last page for more information

    Copenhagen LabMedLokomotivværk

    stedet · Booth no 6

    Visit our boothLearn tips and tricks from our product specialists

    Attend a demonstration and have a chance to win prices

    See last page for information regarding address and tickets

    Capillary electrophoresisFragment Analyzer – the perfect tool for your DNA and RNA analysis

    Liquid handlingCyBi®-SELMASemi-automatic 96- or 384-fold pipettor

    Molecular biologySee the newest clever gadgets for visualization of DNA

    The world’s most effi cient polymerases Experience the Kapa Biosystems solutions fi rst hand

    Lab instrumentsSee and try the hot new labora-tory instruments from BioSan

    Diagnostics- and research kits• ELISA• PCR • NGS kits

    Research reagents for• Glycobiology, • Cell biology • Flow cytometry

    Liquid handlingCyBi®-FeliXCompact pipetting platform for fully automated single- to multichannel liquid handling

    New Tristar² S Multilabel reader with monochromator- great introduction discounts

    Multilabelreader Sense: Microplate reader with touchscreen – it has never been easier

    ELISA • Crocodile ELISA mini workstation

    – Run your ELISA for you• Plate washers 8 and 96 channel

    – Also good for Plate coating• ELISA Readers & automation –

    LED based solutions• ELISA Reagents – Ready to use

    and safe

    Electrophoresis and Fractionation• MW protein fractionator Gel-

    Free8100 – An ideal pre MS analysis tool

    • RunBlue SDS-PAGE – Precast gels with superior resolution and so strong that they show no tears!

    • InstantBlue – The 15 min Coomassie – Quick and Easy gel staining

    Copenhagen LabMed October 7-9, 2014

    reagents for

    • Flow cytometryMost of the news will also be shown at Lab14 in Norway, see last page for more information

    Copenhagen LabMedLokomotivværk

    stedet · Booth no 6

    What’s on display?What’s on display?

    4 Kem-En-Tec Nordic · Finland +358 9594822 · Sweden/Norway +46 18 149000 · Denmark +45 3927 1777

  • Visit our booth Learn tips and tricks from our product specialists

    Attend a demonstration and have a chance to win prices

    See last page for information regarding address and tickets

    Liquid handlingCyBi®-SELMA Semi-automatic 96- or 384-fold pipettor

    Diagnostics- and research kits• ELISA• PCR • NGS kits

    Research reagents for• Glycobiology, • Cell biology • Flow cytometry

    Liquid handlingCyBi®-FeliX Compact pipetting platform for fully automated single- to multichannel liquid handling

    MultilabelreaderSense: Microplate reader with touchscreen – it has never been easier

    Copenhagen LabMed October 7-9, 2014

    Most of the news will also be shown at Lab14 in Norway, see last page for more information

    Visit our booth Learn tips and tricks from our product specialists

    Attend a demonstration and have a chance to win prices

    See last page for information regarding address and tickets

    Capillary electrophoresisFragment Analyzer – the perfect tool for your DNA and RNA analysis

    Liquid handlingCyBi®-SELMASemi-automatic 96- or 384-fold pipettor

    Molecular biologySee the newest clever gadgets for visualization of DNA

    The world’s most effi cient polymerases Experience the Kapa Biosystems solutions fi rst hand

    Lab instrumentsSee and try the hot new labora-tory instruments from BioSan

    Diagnostics- and research kits• ELISA• PCR • NGS kits

    Research reagents for• Glycobiology, • Cell biology • Flow cytometry

    Liquid handlingCyBi®-FeliXCompact pipetting platform for fully automated single- to multichannel liquid handling

    New Tristar² S Multilabel reader with monochromator- great introduction discounts

    Multilabelreader Sense: Microplate reader with touchscreen – it has never been easier

    ELISA • Crocodile ELISA mini workstation

    – Run your ELISA for you• Plate washers 8 and 96 channel

    – Also good for Plate coating• ELISA Readers & automation –

    LED based solutions• ELISA Reagents – Ready to use

    and safe

    Electrophoresis and Fractionation• MW protein fractionator Gel-

    Free8100 – An ideal pre MS analysis tool

    • RunBlue SDS-PAGE – Precast gels with superior resolution and so strong that they show no tears!

    • InstantBlue – The 15 min Coomassie – Quick and Easy gel staining

    Copenhagen LabMed October 7-9, 2014

    Most of the news will also be shown at Lab14 in Norway, see last page for more information

    Copenhagen LabMedLokomotivværk

    stedet · Booth no 6

    Visit our boothLearn tips and tricks from our product specialists

    Attend a demonstration and have a chance to win prices

    See last page for information regarding address and tickets

    Capillary electrophoresisFragment Analyzer – the perfect tool for your DNA and RNA analysis

    Liquid handlingCyBi®-SELMASemi-automatic 96- or 384-fold pipettor

    Molecular biologySee the newest clever gadgets for visualization of DNA

    The world’s most effi cient polymerases Experience the Kapa Biosystems solutions fi rst hand

    Lab instrumentsSee and try the hot new labora-tory instruments from BioSan

    Diagnostics- and research kits• ELISA• PCR • NGS kits

    Research reagents for• Glycobiology, • Cell biology • Flow cytometry

    Liquid handlingCyBi®-FeliXCompact pipetting platform for fully automated single- to multichannel liquid handling

    New Tristar² S Multilabel reader with monochromator- great introduction discounts

    Multilabelreader Sense: Microplate reader with touchscreen – it has never been easier

    ELISA • Crocodile ELISA mini workstation

    – Run your ELISA for you• Plate washers 8 and 96 channel

    – Also good for Plate coating• ELISA Readers & automation –

    LED based solutions• ELISA Reagents – Ready to use

    and safe

    Electrophoresis and Fractionation• MW protein fractionator Gel-

    Free8100 – An ideal pre MS analysis tool

    • RunBlue SDS-PAGE – Precast gels with superior resolution and so strong that they show no tears!

    • InstantBlue – The 15 min Coomassie – Quick and Easy gel staining

    Copenhagen LabMed October 7-9, 2014

    reagents for

    • Flow cytometryMost of the news will also be shown at Lab14 in Norway, see last page for more information

    Copenhagen LabMedLokomotivværk

    stedet · Booth no 6

    What’s on display?What’s on display?

    Kem-En-Tec Nordic · [email protected] · www.kem-en-tec-nordic.com 5

  • 6 Kem-En-Tec Nordic · Finland +358 9594822 · Sweden/Norway +46 18 149000 · Denmark +45 3927 1777

    Visit us at CPH LabMed and let us help you find the kits and reagents for your diagnostic needs

    Diagnostic kits for:• Diabetes• Obesity• Microbiology• Infectious diseases• Autoimmunity• Cancer research• and much more

    Research kit• ELISA and RIA

    Controls• Ready to use vials for many

    instruments

    Media• 3D• Stemcells

    Research and Clinical diagnostics

    - Check our kits and reagents

    We represent leading suppliers of:• Non-toxic transfection reagent• Glycobiology reagents• Flowcytometri reagents• Research ELISA kits

  • Kem-En-Tec Nordic · [email protected] · www.kem-en-tec-nordic.com 7

    From Berthold Technologies can we offer you all from a simple-easy to use-ELISA reader, to the high end MIthras² multilabel reader,with filters and double monochromators.

    See the new Trista² S model with monochromator at CPH LabMed booth 6, and get information about how to get up to 35% introduction discount

    Single- or multi label reading in 1 to 1536 wells plates?It makes good sense to come and try how easy it is to use Hidex new Sense multilabel reader with touch screen - perfect for multiuser purpose

    Tired of moving plates? - or pipetting by hand?

    CyBi®-Selma- 96 or 384 channel pipettor• Simple and intuitive handling with touch

    screen• Easy changing of tips• Serial dilution possible with special 8 channel

    magazine• Quick start: no programming or plate defini-

    tions required – remembers height of plate!• Two working positions for fast liquid transfer

    CyBi®-Felix• Compact pipetting platform for fully

    automated single and multichannel liquid handling

    See it at CPH LabMedBooth: 6

    See it at CPH LAbMed Booth: 6

    CyBio AG20 years of German Quality

  • 8 Kem-En-Tec Nordic · [email protected] · www.kem-en-tec-nordic.com

    Our team of BioScience specialists – ready to help!

    Meet our competent and growing team!

    We welcome our new product specialist Håkan Rundgren, MSc in Biotechnology. Håkan has been in the BioScience world since 1995 and are looking

    forward to help our customers in Norway & Sweden!

    Exhibitions in Denmark/South Sweden and Norway! Visit us and win nice prices and knowledge!

    Copenhagen LabMed 7.-9. October – in parallel with DEKS and LSB conferences.

    Otto Busses Vej 5A, 2450 København SVBooth: Nr 6 - Kem-En-Tec Nordic, Competent Bioscience since 1987!

    Lab14 28.-30. October Norge’s Varemesse, Lillestrøm Booth: B02-32- Kem-En-Tec Nordic, Competent Bioscience since 1987!

    Tickets

    Tickets

    OBS!

    New webshop

    for easy ordering

    The first 30 customers through

    the web shop will win a nice price

    – 1 “tower” box of delicious liquorice!

    Check it out – create user and start buying – easy!

    www.kem-en-tec-nordic.com

    Competition on the stand! Win a romantic dinner for two or cinema tickets!

    Or participate on the stand – throw the dices and win nice prices right away!

    Looking forward to seeing you and good luck!

    Win prices at our stand