no trace from cellulite - karitia drp

V.6

NO TRACE FROM CELLULITE

• Medically, cellulite is primarily a condition of poor microcirculation

that causes damage to the fat tissue under the skin.

• Cellulite is usually found on the thighs and butt, especially in women.

It affects about 80 to 95% of women, regardless of weight. It only

affects 5% of men due to differences in fat levels and hormones.

• It is a complex process which has several causes of diverse nature:

dietetic, vascular, endocrine, lifestyle, etc.

CELLULITE: THE PROBLEM

• Aesthetically, cellulite is a problem consisting in

the visual presentation of weakened, damaged

fatty tissue and fibrous connective tissue in the

shape of lumps and dimples, commonly known as

“orange skin”.

CELLULITE VISIBLE SIGNS

CELLULITE FORMATION MECHANISMS

Vicious Circle

1. Failure of microcirculation system.

2. Leakage of liquid into the surrounding tissue.

3. Adipocytes start to manufacture excess tryglicerides and grow in

size, becoming trapped in the connective tissue.

4. Tissue congestion causes swelling, making it difficult for oxigen

and metabolites to reach the cells and hindering the drainage of

toxins.

5. Connective tissue including elastine and collagen becomes

increasingly damaged.

6. Formation of cellulite nodules.

CELLULITE FORMATION MECHANISMS

MULTIFACTOR PROCESS

• Maturation of adipocytes

• Mobilisation of fat

• Microcirculation

• Inactivation of toxins


Maturation of

adipocytes

Mobilisation of fat

Microcirculation

Inactivation of toxins

4-in-1 Fighting actions

1. Inhibition

2. Lipolytic activity

3. Reactivation

4. Scavenger


• contains multivesicular liposomes of classical anti-cellulite

ingredients combined with the synergistic effect of a GHK

tripeptide.

• Their active ingredients act in 2 different ways: against fat

accumulation (lipolytic effect) and to fight microcirculation

failures responsible for cellulite (venotonic effect).

LIPOLYTIC ACTIVES:

• CAFFEINE: blocks enzymes responsible for destruction of AMPc, which is

involved in triglyceride breakage.

• TEA-HYDROIODIDE: has lipolytic properties by stimulating lipases.

• CARNITINE: enhances triglyceride mobility, accelerating their breakdown.

SCAVENGER ACTIVE:

• GHK (TRIPEPTIDE-1): is a specific savenger of certain by-products of lipidic

peroxidation.

LIPOREDUCTYL® ACTIVES

VENOTONIC ACTIVES:

• ESCIN (AESCULUS HIPPOCASTANUM): is a venotonic and anti-oedematous

ingredient.

• IVY EXTRACT (HEDERA HELIX): contains Hederine, an active saponin

responsible for blood vessel protection and permeability decrease. Ivy

helps to reabsorb the oedemas present in the initial stages of cellulite.

• BUTCHEBROOM EXTRACT (RUSCUS ACULEATUS): decreases capillary

permeability due to its content in flavonoids.

• CAFFEINE: it also possesses vasodilator properties, increasing blood flow.

LIPOREDUCTYL® ACTIVES

LIPOREDUCTYL® EFFICACY

IN VIVO EFFICACY

• Evaluation of the anti-cellulite effects

IN VITRO EFFICACY

• Inhibition of adipocyte maturation

• GHK: Capture of toxic by-products

• Percutaneous absorption

INHIBITION OF ADIPOCYTE MATURATION

• A cell culture model of human preadipocytes was stimulated to differentiate

adipocytes.

• Lipid droplets were visualised by phase contrast microscopy and quantified by image

analysis.

LIPOREDUCTYL® concentration

The number of adipocytes per area decreased

after treatment with LIPOREDUCTYL®.

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

Control - 0 µg 0.1 μg/mL 1 μg/mL 10 μg/mL 100 μg/mL

Num

ber

of

adip

ocyte

s

INHIBITION OF ADIPOCYTE MATURATION

Control 0 g/mL 0.1 g/mL

1 g/mL 10 g/mL 100 g/mL

CELLULITE

PREVENTION

The dose-dependent reduction in adipocytes and consequently in

lipid droplets (in blue) confirms:

• LIPOREDUCTYL® effectiveness.

• LIPOREDUCTYL® does not target any other cell type.

• Tissues affected by skin aging contain elevated levels of reactive α,β-unsaturated

aldehydes, such as 4-hydroxy-2-nonenal (HNE), malondialdehyde (MDA) and acrolein

(ACR), all of which are well known by-products of lipid peroxidation.

• GHK is a potent aldehyde-sequestering agent.

GHK: CAPTURE OF TOXIC BY-PRODUCTS

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

1:15 1:30 1:200 1:400

Quenched a

ldehyde (

%)

Quenching activity GHK vs HNE

1 h 2 h

0.0

20.0

40.0

60.0

80.0

100.0

120.0

1:15 1:30

Quenched a

ldehyde (

%)

Quenching activity GHK vs Acrolein

1 h 2 h

PERCUTANEOUS ABSORPTION

A skin penetration test was performed using a 3D in

vitro skin model (from Skinethic® Laboratories, Nice).

Caffeine and Escin were monitored for penetration

together with LIPOREDUCTYL® and compared with

free actives.

• Caffeine penetration increased 5-fold

compared to pure caffeine.

• Escin penetration increased almost 8-fold

compared to pure escin.

LIPOREDUCTYL®’s liposomal formulation

favours epidermal penetration.0.0

1.0

2.0

3.0

4.0

5.0

4 h 16 h

Abso

rbed e

scin

(%

)

Escin LIPOREDUCTYL®

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

2 h 4 h 16 h

Abso

rbed c

aff

ein

e (

%)

Caffeine LIPOREDUCTYL®

EVALUATION OF THE ANTI-CELLULITE EFFECTS

• In vivo test performed in 20 female volunteers with cellulite imperfections aged 18 to 70.

• A gel containing 7% LIPOREDUCTYL® was applied for 60 days on a daily basis on specific

body areas (Glute SX, Glute DX, Femoris Post. SX, Femoris Post. DX, Femoris Ant. SX, Femoris Ant. DX).

• Instrumental and dermatological parameters were assessed.

INSTRUMENTAL assessment

Buttocks and thigh circumferences

Body fat mass (STA/BIA instrument)

Extracellular water (STA/BIA instrument)

Skin moisture (corneometer)

Skin elasticity (cutometer)

DERMATOLOGICAL assessment

Microcirculation (video capillaroscopy)

Orange skin

Nodule’s presence

Skin smoothness

Skin compactness

Complexion

• Buttocks: 60% decreased 0.5 to 1 cm

15% decreased 2.0 to 3.0 cm.

• Thigh: 85% decreased 0.5 to 1 cm.

• Body Fat: 50% decreased 0.6 to 1.4 kg

15% decreased 2.0 to 3.0 kg.


93.6

93.8

94.0

94.2

94.4

94.6

94.8

95.0

95.2

0 days 60 days

Butt

ock c

ircum

fere

nce

(cm

)

54.8

54.9

55.0

55.1

55.2

55.3

55.4

55.5

55.6

55.7

0 days 60 days

Thig

h c

ircum

fere

nce

(cm

)14.6

14.8

15.0

15.2

15.4

15.6

15.8

0 days 60 days

Body f

at

mass

(kg)

• ECW: 60% of volunteers decreased

0.5 to 1.6 litres.

• Elasticity: increase of 17.5% after

30 days and of 24% after 60 days.

• Hydration: the moisturising index

increased by 9.5%.


12.4

12.6

12.8

13.0

13.2

13.4

0 days 60 days

Extr

acellula

r w

ate

r(l

)

0.3

0.4

0.5

0.6

0.7

0.8

0.9

0 days 15 days 30 days 60 days

Skin

ela

stic

ity (

cuto

mete

r re

adin

g)

33.0

34.0

35.0

36.0

37.0

38.0

39.0


Skin

mois

turi

sing

(corn

eom

ete

r re

adin

g)

• Microcirculation: 60% showed improvement in

their polygonal network. Another 35% had a

slight improvement.

• Orange skin: 75% improved with 25%

presenting a fairly good improvement.

• Nodules: 95% improved, and 40% experienced

a fairly good improvement.


0.0

0.5

1.0

1.5

2.0

2.5

3.0


Skin

mic

rocir

cula

tion

(score

)

0.0

0.5

1.0

1.5

2.0

2.5


Ora

nge s

kin

(sc

ore

)

0.0

0.5

1.0

1.5

2.0

2.5


Pre

sence o

f nodule

s (s

core

)

• Compactness: 55% showed a compact and

elastic skin after treatment.

• Smoothness: 65% showed smooth skin after

the test.

• Complexion: 65% showed a rosy complexion

and improved microcirculation.


0.0

0.5

1.0

1.5

2.0

2.5

3.0


Skin

sm

ooth

ness

(sc

ore

)

0.0

0.5

1.0

1.5

2.0

2.5

3.0


Skin

com

pactn

ess

(sc

ore

)

0.0

0.5

1.0

1.5

2.0

2.5

3.0


Com

ple

xio

n (

score

)

All pictures show an improvement

in the structure of microcapillaries

and decrease in microhaemorrages.

0 days 60 days

VIDEO CAPILLAROSCOPY

• Rosier complexion.

• Less evident presence of nodules.

• Improvement in the degree of

orange skin.

MACROSCOPIC TRANSLATION


CONCLUSIONS

• Shows four specific cellulite-fighting actions:

Revolutionary cellulite prevention due to action on the maturation process of

adipocytes.

Traditional lipolytic activity provided by classic anti-cellulite ingredients.

Activation of microcirculation due to the powerful venotonic profile of several actives.

The GHK peptide acts as an efficient scavenger for by-products of lipidic peroxidation.

• These properties translate into visual effects:

Enhanced microcirculation improves the skin’s complexion.

Improved skin hydration and skin elasticity.

Reduction in thigh and buttock circumference.

Reduction in extracellular water and body fat mass.

Disclaimer:

While the claims and supporting data provided in this publication are believed to be reliable and they are presented free and for guidance only, there are no warranties of any kind. All expressed and implied warranties are disclaimed. The recipient is

solely responsible for ensuring that products marketed to consumers comply with all relevant laws and regulations. LIPOTEC is the exclusive holder of the both industrial and intellectual property rights identified herein. Recipient of this publication

agrees to indemnify and hold harmless each entity of the LIPOTEC organization for any and all regulatory action arising from recipient’s use of any claims or information in this publication, including, but not limited to, use in advertising and finished

product label claims, and not present this publication as evidence of finished product claim substantiation to any regulatory authority.

no trace from cellulite - karitia drp

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