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of the German Stem Cell Network (GSCN)November 3-5, 2014German Cancer Research Center (DKFZ)Heidelberg

2nd Internati onal Annual Conference

Program & Abstracts

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www.gscn.org

2nd International Annual Conference of the GSCN

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2nd International Annual ConferenceGerman Stem Cell Network (GSCN)

German Cancer Research Center (DKFZ)Heidelberg

November 3 – 5, 2014

Organization:German Stem Cell Network (GSCN)c/o Max Delbrück Center (MDC)Robert-Rössle-Str. 1013125 Berlin

phone: +49 30 9406 2487fax: +49 30 9406 2486e-mail: [email protected]: www.gscn.org

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2nd International Annual Conference of the GSCN November 3 – 5, 2014 I DKFZ Heidelberg, Germany

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Dear Stem Cell Colleagues,

Itisagreatpleasuretowelcomeyoutothe2nd International Annual Conference of the German Stem Cell Network (GSCN) at the German CancerResearchCenter(DKFZ)inHeidelberg.

Themostimportantdevelopmentduring2014hasundoubtedlybeenthecontinuedcommitmentoftheBundesministeriumfürBildungundForschung (BMBF) to support the GSCN for threemore years. ThiswaspossibleduetotheoverwhelmingGerman-widesupportfromallof our members and the success of the 1st Annual GSCN Conference inBerlinlastautumn.Today,weareveryexcitedtostartthesecondGSCNconferencewithapackedprogramandevenmorecutting-edgeresearchaswellasavarietyofworkshops.

The 2nd International Annual Conference of the GSCNprovidesanopportunityforscientists,cli-nicians,educatorsandindustryprofessionalstosharenewdata,learnfrompeers,anddiscoverglobaladvanceswithinthestemcellfield.Topicscoverthelatestfindingsinstemcellbiology,fromgeneticandepigeneticmechanismsofreprogrammingandmaintenanceofpluripotency,tothemolecularbasisofself-renewalanddifferentiation.Theconferencewillfostertheinter-actionbetweenresearchersworkingondifferenttypesofstemcells,includingembryonicstemcells,stemcellsduringdevelopmentandinadultorgansaswellascancerstemcells.Moreover,theapplicationofstemcellsinregenerativetherapies,diseasemodelinganddrugdevelopmentwillrepresentthetranslationalandappliedangleofthefield.Finally,sessionsonstrategicissuesrelatingtostemcellresearchsuchascareerdevelopment,publicoutreach,fundingpoliciesandclinicaltrialsusingstemcellswillcompletetheprogram.

Withmanyspeakersselectedfromthebestabstracts,theconferenceisaplatformfortheentirescientific communityprovidinguniqueopportunitiesespecially for young talented scientists.TheprogramisflankedbyafewselectedKeynotelecturesgivenbyinternationalleadersintheirfield.TheGSCNconferencewillbedirectlyfollowedbytheJointSatelliteSymposiumonNeuralStemCellswhichlastsuntilNovember6th(freeofchargeforGSCNConferenceparticipants).

Wearelookingforwardtothreebusydayspackedwithinput,discussion,newinsightsandnewcontacts,therefreshingofoldconnectionsandaconstantlygrowingfascinationforourresearchwiththemostexcitingcellsweknowof:stemcells.Iamdelightedtoseeallofyouinmyhome-townofHeidelbergandwishyouastimulatingandproductivemeeting.

Bestwishes,

AndreasTrumpp(GSCNActingPresident)

For the program committeeDaniel Besser (Berlin) • ThomasBraun(BadNauheim)• Oliver Brüstle (Bonn) • Micha Drukker (Munich) • Ulrich Martin(Hannover) • AnaMartin-Villalba(Heidelberg) • AndreasTrumpp(Heidelberg)

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Table of contents

Welcome address �� 2

Conference information �� 4

Workshops �� 5

Awards �� 6

Outreach events �� 8

Social event �� 9

Floor plan �� 10

Program overview �� 12

Program �� 14

Speaker abstracts �� 27Keynotelectures �� 28Selectedpresentations �� 37Companypresentations �� 87

Poster presentations �� 99Poster session IPluripotencyandembryonicstemcells(P001–P017) �� 100Programingandreprograming(P018–P038) �� 118Stemcellsindevelopment(P039–P061) �� 140Somaticstemcells(P062–P072) �� 164Hematopoieticstemcells(P073–P097) �� 174

Poster session IIStemcellsinregenerativetherapies(P098–P129) �� 202Stemcellsindiseasemodelinganddrugdevelopment(P130–P161) �� 238Stemcellsindisease:Cancerstemcells(P162–P178) �� 274Computationalstemcellbiology(P179–P187) �� 292

Author index �� 301

List of participants �� 304

Supporters & exhibitors �� 316


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Conference information

Venue:DKFZCommunicationCenter,HeidelbergImNeuenheimerFeld28069120 Heidelberg

Date: Monday,November3toWednesday,November5,2014

Registration

Theregistrationfeeincludesattendanceatallscientificsessions,November3–5,conferencedocuments,namebadge,finalprogramandabstractbook,conferencebag,Networking Evening,coffeebreaks,lunch,dinnerandfreeinternetaccess.

Posters:Posterswillbedisplayedduringthemeetingin2sessionsintheexhibitionarea.Thesizeofasinglepostershouldnotexceed1mx1,20m(width / height).Youwillfindthenumberofyourposterinthisabstractvolume.Accordingtothisnumber,youmaymountyourposterintheexhibitionarea.

Posters scheduled for Poster session I(PosternumbersP001–P097)canbeviewedonMonday,November3,2014.Thepostersinthissessionshouldbeset-upon Monday,12:00h–16:00handremovedlatestonTuesday13:00h.

Posters scheduled for Poster session II(PosternumbersP098–P187)canbeviewedon Tuesday,November4,2014.Thepostersinthissessionshouldbeset-upon Tuesday,13:00h–17:00handremovedlatestonWednesday14:00h.

Internet: InternetaccessviaWirelessLANisfreeofchargeintheCommunicationsCenter.Username: g-guest46; Password: mosamoli

GSCN navigator:InstalltheofficialappfortheGermanStemCellNetwork(GSCN)andeventguidefortheGSCNConference2014onyoursmartphone:• Overviewoftheprogramandportfolio• Createyourownprogramfortheevent(MyPlan): bookmarkthesessionstoattend

• Networkingtool:findotherparticipantsandschedule meetingswiththem

• Usemaptonavigatetothevenue

Regular fee: 550 € Student fee: 300 € Dayticket(onsite): 200 €Regular member fee: 375 € Student member fee: 200 €

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Information on the workshops at the GSCN Conference 2014

Banking of human pluripotent stem cells – workshopZMBH,Tuesday,November4th;14:30–15:30h(Chair: Andreas Kurtz / Joana Namorado)

Humanembryonicstemcellsandinducedhumanpluripotentstemcells(PSC)haveopenedawiderangeofpossibilitiesintherapeuticalsettingsandfordiseasemodelinganddrugdevelop-ment.Largescalebankingofthesecellshasbecomeamajortrendworldwide.SeveralPSCbank-ingprojectsinEuropearefundedbytheEuropeanCommissionandpharmaceuticalindustryforpreclinicaldrugassessment,mode-of-actionstudiesandtherapeuticuse.Globalregistrationofpluripotentstemcellswillbeinstrumentalfortheefficientfutureuseoftheseresources.Thisworkshopwillshowthecurrenteffortsonderivation,bankingandregistrationofhumanPSCs.

Clinical relevance of animal models in stem cell research and regenerative medicineLectureHall,Wednesday,November5th;11:00–12:30h(Chair: Georg Duda / Frank Emmrich)(Workshopofthe‘RegenerativeMedicineInitiativeGermany(RMIG)’)

Suitableanimalmodelsareofcentral importancetothedevelopmentofeffectivetherapies,especiallyinthefieldofstemcellresearchandregenerativemedicine.Inbasicresearch,lowervertebratesandsmallrodentsunderstandard‘SafetyPathogenFree’housingconditionsarethepreferredmodelorganisms,whilelargeanimalmodelsaswellassmallanimalmodelsrepre-sentanimportantstepinthepreclinicalevaluationofcell-basedtherapies.TheinstitutesintheRMIGareworkingto improvethequalityandpredictabilityofpre-clinicalanimalmodels forstemcellandregenerativetherapies.Basedontheexpertiseintheinstitutes,apanelaimstoenterintoacriticaldiscussionontheclinicalrelevanceofanimalmodelsystems.

Big pharma and the stem cell field – matching expectations?K1+2,Wednesday,November5th;11:00–12:30h(Organizers: Ira Herrmann / Oliver Brüstle)(WorkshopoftheStemCellNetworkNorthRhineWestphalia)

Diseasemodeling and drug development are consideredmajor deliverables of translationalstemcellresearch.Howevertheexpectationsofthepharmaceuticalindustryfromthisrapidlyevolvingareahaveyettobedefined.Thisworkshopfeaturesexpertsfromdrugdiscoveryandcompoundscreeningprogramsoflargepharmaceuticalcompanies.It’sobjectiveistoprovidethestemcellcommunitywitharealworldviewonthisvibranttopicandenablefutureinterac-tionsinthisscientificallyandeconomicallyfulminatingfield.

Panel:OliverBrüstle;LIFE & BRAIN GmbH, Bonn RalfHeilker;Boehringer Ingelheim Pharma GmbH & Co. KG, Div. Research Germany JörgHüser;Bayer Pharma AG, Lead Discovery Wuppertal BertKlebl;Lead Discovery Center GmbH, Dortmund Moderation:Ira Herrmann; Stem Cell Network NRW, Düsseldorf


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GSCN Travel Awards

ThefollowingparticipantshavebeenselectedfortheGSCN travel awards:• CantasAlev(RIKENCenterforDevelopmentalBiology,Kobe,Japan)• JanineMüller(UniversityofBielefeld)• SusanneRahmig(TechnicalUniversityDresden)• YuvalRinkevich(StanfordUniversity,California,UnitedStates)

Poster awards

Theposter awardsaresupportedbyPeprotech GmbH.

There will be two poster awards for each poster session:

Poster session I:Monday,November3rd,2014,16:15–18:15h • Pluripotencyandembryonicstemcells(P001–P017)• ProgramingandReprograming(P018–P038)• Stemcellsindevelopment(P039–P061)• Somaticstemcells(P062–P072)• Hematopoieticstemcells(P073–P097)

Evennumbers:willbepresentedfrom16:15–17:15hUnevennumbers:willbepresentedfrom17:15–18:15h

Poster session II: Tuesday,November4th,2014,17:15–19:15h• P098–P129:Stemcellsinregenerativetherapies• P130–P161:Stemcellsindiseasemodelinganddrugdevelopment• P162–P178:Stemcellsindisease:Cancerstemcells• P179–P187:Computationalstemcellbiology

Evennumbers:pleasepresentyourposterfrom17:15–18:15hUnevennumbers:pleasepresentyourposterfrom18:15–19:15h

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Explaining science and motivating people – outreach activities

Presentation of outreach materials and formats by the working group ‘Public engagement and outreach activities’ (Tobias Cantz / Ira Herrmann)

Tuesday,November4th,13:15–14:15, roomK1+2andWednesday,November5th,13:45–14:45, roomK1+2

Stopbyandgettoknowdifferenttools,activities,materials and games to communicate stem cell researchtodifferentaudiences.Thenext‘openscienceday’or‘nightofsciences’inyourinstituteisjustaroundthecorner!

Public outreach eventStammzellen als Chance – Realität und PerspektivenPodiumsdiskussionzuraktuellenForschunginLaborundKlinik

Mittwoch,5.November,18:30–20Uhr, HörsaalDKFZ

Krebs- und neurodegenerative Erkrankungenwie Alzheimer und Parkinson nehmen welt-weit zu. Die Stammzellforschung weckt großeHoffnungen bei Patienten: Doch wo steht dieStammzellforschung?BeiderTherapievonLeu-kämien gehört die Stammzelltransplantationzum erfolgreichen Behandlungsrepertoire. Fürandere Erkrankungen dienen Stammzellen alsModell in der Forschung oder werden gar alsbedeutend für den Krankheitsverlauf, z.B. beibestimmtenKrebsarten,gehandelt.

DreiPionierederStammzellforschungstellenZusammenhängedarunddiskutieren ihreaktuellenProjektemitdemPublikum:• Prof.Dr.MagdalenaGötz,München• Prof.Dr.AnthonyHo,Heidelberg• Prof.Dr.AndreasTrumpp,Heidelberg

Moderation:Dr.StefanieSeltmann,DKFZ

In collaboration with:

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Social Event

The Networking EveningwithdinnerbuffetandDJisopenforallparticipantsandwilltakeplaceonTuesday,November4th,20:00hintherestaurantMolkenkur.

Address:Schlosshotel Molkenkur Klingenteichstr.31 69117 Heidelberg

Freeshuttlebusestonetworkevening.Buseswillleaveat19:30hfromtheCommunicationCenter.

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Company booth

AMSBiotechnology(Europe)Ltd 6BD Biosciences 5BioFroxxGmbH 7Biomol GmbH 4CellGenix GmbH 12Cenibra GmbH 18Diagenodes.a.BELGIUMEUROPE 19EppendorfAG 15ESSENBioScienceLtd 8FluidigmEuropeB.V. 14LabotectGmbH 1LifeTechnologiesGmbH 9

Company booth

LonzaCologneGmbH 16MiltenyiBiotecGmbH 22NanostringTechnologies 2GermanyGmbHPELOBIOTECHGmbH 11PeproTechGmbH 13PromoCell GmbH 21STEMCELLTechnologiesSARL 3TakaraBioEurope,SAS 20ThermoScientific 10VWRInternationalGmbH 17

Floor plan

Legend

Tent

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Tent

First floor – Communication Center

Ground floor – Communication Center

Posterexhibition

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ZMBH

K2K1

Lecture Hall

Stairs to the firstfloor


MONDAY, November 3rd, 2014 Lecture hall K1 K2 ZMBH11:00 – 12:00 Registration12:00 – 12:30 Opening12:30 – 13:15 KeynotelectureI

Eduard Batlle13:15 – 14:00 KeynotelectureII

PhilippeMenasché14:00 – 14:30 Coffeebreak / industryexhibition14:30 – 16:00 ConcurrentscientificworkinggroupsessionI

StemcellsinregenerativetherapiesI Stem cells in disease: cancer stem cells

Computatational stemcellbiology

Somaticstem cells

16:00 – 16:15 Coffeebreak / industryexhibition16:15 – 18:15 Poster session I P001–P017:Pluripotencyandembryonicstemcells

P018–P038:Programingandreprograming P039–P061:Stemcellsindevelopment P062–P072:Somaticstemcells P073–P097:Hematopoieticstemcells

18:15 – 19:15 Dinner19:15 – 20:45 GSCNGeneralMembershipmeeting20:45 – 21:30 Informal GSCN Get-together

Program overview

TUESDAY, November 4th, 2014Lecture hall K1+2 ZMBH

09:00 – 10:30 ConcurrentscientificworkinggroupsessionIIStem cells in disease model-inganddrugdevelopment

Pluripotencyandembryonicstem cells

Stemcellsindevelopment

10:30 – 11:15 Coffeebreak / industryexhibition11:15 – 12:50 Industrysession:“TechnologiesfromGSCNindustrypartners”

PeproTech Eppendorf CellGenix

LifeTechnologies apceth STEMCELLTechnologies

Nanostring Fluidigm Lonza

12:50 – 14:30 Lunchbuffet / industryexhibition / posterviewing13:15 – 14:15 Outreach materials for stem

cell research14:30 – 15:30 ConcurrentstrategicworkinggroupsessionA Workshop

CarreerDevelopment ClinicaltrialsandregulatoryaffairsI

Largescalebankingof humanpluripotentstemcells

15:30 - 15:45 Short break15:45 – 16:45 ConcurrentstrategicworkinggroupsessionB

Stem cell technologies ClinicaltrialsandregulatoryaffairsII

Fundingopportunitiesforyoungandadvancedscientists

16:45 – 17:15 Coffeebreak / industryexhibition

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16:45 – 17:45 Meet-the-experttable:Geneticengeneering(DKFZMensa)17:15 – 19:15 Poster session II P098–P129:Stemcellsinregenerativetherapies

P130–P161:Stemcellsindiseasemodelinganddrugdev. P162–P178:Stemcellsindisease:Cancerstemcells P179–P187:Computationalstemcellbiology

19:15 – 20:00 Freebusshuttletonetworkingevening20:00 – 01:00 NetworkingEveningwithdinnerbuffetatSchlosshotel“Molkenkur”

(Klingenteichstr.31,69117Heidelberg)

WEDNESDAY, November 5th, 2014Lecture hall K1+2 DKFZ SR 4th floor

09:00 – 10:30 ConcurrentscientificworkinggroupsessionIIIHematopoieticstemcells Programmingandrepro-

grammingStemcellsinregenerativetherapiesII(Meetattheregistrationdesk)

10:30 – 11:00 Coffeebreak / IndustryexhibitionZMBH

11:00 – 12:30 ConcurrentworkshopsClinical relevance of animal models in stem cell research andregenerativemedicine

Bigpharmaandthestemcellfield–matchingexpec-tations?

ProceedingsoftheGSZ

12:30 – 12:45 Short break12:45 – 13:30 KeynotelectureIII

ShahragimTajbakhsh13:30 – 15:00 Lunchbuffet / industryexhibition13:45 – 14:45 Meet-the-experttable:Laserprinting&Reprogrammingtechnologies(DKFZMensa)13:45 – 14:45 Outreach materials for stem

cell research15:00 – 15:15 Posterawardceremony15:15 – 15:30 Outlook and closing remarks15:30 – 16:15 KeynotelectureIV

JürgenKnoblich16:15 – 17:00 KeynotelectureV

Amelia Eisch17:00 – 17:45 KeynotelectureVI

SebastianJessberger17:45 End of GSCN conference17:45 – 18:30 CoffeebreakGSCNsatelliteevent18:30 – 20:00 GSCNpublicoutreachevent:

StammzellenalsChance–RealitätundPerspektiven

20:00 – 21:00 Receptionpublicoutreachevent

Generalmeeting Opening / Closing Workshops Keynotelectures

Concurrentscientificsessions Concurrent strategic sessions Public event Networking evening

Outreachinformation Meet-the-experttable Poster Session IndustrysessionLE

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Program

MONDAY, November 3rd

11:00–12:00 Registrationandlightlunch

OpeningLecture hall:12:00–12:05 WelcometotheGermanCancerResearchCenter(DKFZ):

Otmar Wiestler(ChairmanandScientificDirector,DKFZ)12:05–12:15 Andreas Trumpp(ActingPresident,GSCN)12:15–12:30 Openingremarks

Bärbel Brumme-Bothe(DepartmentHead“LifeSciences”,BMBF)12:30–13:15 Keynote lecture I

K1-Exploringthehierarchicalorganizationofcolorectalcancer Eduard Batlle;InstituteforResearchinBiomedicineBarcelona,Spain (Chair: Andreas Trumpp) supported by Eppendorf AG

13:15–14:00 Keynote lecture II K2-MyocardialregenerationbyESC-derivedcardiacprogenitors Philippe Menasché;HospitalEuropeanGeorgePompidou,Paris,France (Chair: Ulrich Martin)

14:00–14:30 Coffeebreak / industryexhibition

Concurrent scientific working group session ILecture hall: Stem cells in regenerative therapies I (chair: Ulrich Martin / Hans-Dieter Volk)14:30–14:50 Stemcellsinregenerativetherapies–Introduction

Ulrich Martin;Rebirth / HannoverMedicalSchool14:50–15:05 T01-Intranasaldeliveryofmigratoryneuralstem/progenitorcells:Anon-

invasivepassagetotherapeuticallytargetintracerebralbraintumors Nils Ole Schmidt;UniversityMedicalCenter,Hamburg-Eppendorf

15:05–15:20 T02-MicroRNA-181apromoteshumanneuronaldifferentiationbydown-regulatinggenesinvolvedinneuralstemcellmaintenance Laura Stappert;InstituteofReconstructiveNeurobiology,Bonn

15:20–15:35 T03-Pulmonarytransplantationofmultipotent-orpluripotent-stemcellderivedmacrophagesasanoveltreatmentoptionforpulmonaryalveolarproteinosis Nico Lachmann; Hannover Medical School

15:35–15:50 T04-HumaniPSC-derivedMSC-likeprogenitorcells:functionaldifferencesfromprimaryMSCsaffectingtheiruseincartilageregenerativemedicine Solvig Diederichs;UniversityHospitalHeidelberg

15:50–16:00 Groupdiscussion

Conferenceprogram 15

Room K 1: Stem cells in disease: cancer stem cells (chair: Thomas Brabletz / Andreas Trumpp)14:30–14:50 Conceptsofcancerstemcells–Introduction

Andreas Trumpp;GermanCancerResearchCenter,Heidelberg14:50–15:05 T05-Shp2Signalingisessentialtothesuppressionofsenescenceinmammary

gland cancer stem cells in mice Linxiang Lan;MaxDelbrückCenter,Berlin

15:05–15:20 T06-Humanizationofmousemodelsforhumanbreastcancerusingmesenchymalstemcells Maxine Silvestrov;ExperimentalPharmacology&Oncology(EPO)GmbH,Berlin

15:20–15:35 T07-Targetingthekinometoinducedifferentiationinhumanbreastcancerstem cells Jochen Maurer;UniversityMedicalCenterFreiburg

15:35–15:50 T08-CD151+cellsdrivetumor-initiation,hierarchicalgrowthandproliferation-associatedsignalinginpatient-derivedmodelsofserousovariancancer Franziska Zickgraf;GermanCancerResearchCenter,Heidelberg


Room K 2: Computational stem cell biology (chair: Georg Fuellen / Ingo Roeder)14:30–14:45 Computationalstemcellbiology–Overviewandintroduction

Ingo Roeder;DresdenUniversityofTechnology14:45–14:55 T09-Whatmakesstemcellsmove?Studyingstemcellmigrationinvitro

(back-to-backpresentationwithT10) Michael Ansorge;LeipzigUniversity

14:55–15:05 T10-Whatmakesstemcellsmove?Studyingstemcellmigrationinsilico (back-to-backpresentationwithT09) Axel Krinner;DresdenUniversityofTechnology

15:05–15:20 T11-Characterizingtranscriptionalcell-to-cellheterogeneitiesduringstemcelldifferentiation Fabian Theis;HelmholtzCenterMunich

15:20–15:35 T12-Anetwork-basedstrategytodirectcellfatedetermination Antonio del Sol;LuxembourgCenterforSystemsBiomedicine

15:35–15:50 T13-Computationalidentificationofpluripotencynetworks Maryam Nazarieh;SaarlandUniversity,Saarbrücken


ZMBH (groundfloor): Somatic stem cells (chair: Thomas Braun / Ana Martin-Villalba)14:30–14:50 Studiesonsomaticstemcells–anintroduction

Thomas Braun;MaxPlanckInst.f.HeartandLungRes.,BadNauheim14:50–15:05 T14-TheRNAhelicaseDDX6regulatescell-fatespecificationinneuralstem

cells via miRNAs Sarah Nicklas;LuxembourgCenterforSystemsBiomedicine

15:05–15:20 T15-AngiogenesiscontrolsneuralstemcellexpansionbyregulatingtissueoxygenationandHIFsignaling Christian Lange; VIBKULeuvenCampus,Belgium

Conferenceprogram


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15:20–15:35 T16-Regulationofasymmetric/symmetricstemcelldivisioninhumanepidermis Katharina Nöske;GermanCancerResearchCenter,Heidelberg

15:35–15:50 T17-Clonalheterogeneitywithintheexocrinepancreas Damian Wollny;GermanCancerResearchCenter,Heidelberg



16:15 – 18:15 Poster session I: P001 – P097 supported by Fluidigm Europe B�V� (posterswillbedisplayedinthetent) P001–P017:Pluripotencyandembryonicstemcells P018–P038:Programingandreprograming P039–P061:Stemcellsindevelopment P062–P072:Somaticstemcells (posterswillbedisplayedinfrontofroomK1+2) P073–P097:Hematopoieticstemcells

Evennumbers:pleasepresentyourposterfrom16:15–17:15 Unevennumbers:17:15–18:15

18:15–19:15 Dinner

Lecture hall:19:15–20:45 GSCNGeneralMembershipMeeting20:45–21:30 Get-together


TUESDAY, November 4th

Concurrent scientific working group session IILecture hall: Stem cells in disease modeling and drug development

(chair: Oliver Brüstle / Karl-Ludwig Laugwitz) supported by Lonza Cologne GmbH

09:00–09:20 Theuseofstemcellsindiseasemodelinganddrugdevelopment–Overview Oliver Brüstle;InstituteofReconstructiveNeurobiology,Bonn

09:20–09:35 T18-ProbingmitochondrialDNAdisordersusingiPSC-derivedneuralprogenitors Alessandro Prigione; MaxDelbrückCenter,Berlin

09:35–09:50 T19-ModelingDravetsyndromeiniPScell-derivedneurons Matthias Hebisch;InstituteofReconstructiveNeurobiology,Bonn

09:50–10:05 T20-Thefunctionalityofstemcellderivedneurons:Howtodefineit? Sebastian Illes; Inst.ofMolecularReg.Medicine,Salzburg,Austria

10:05–10:20 T21-Modelingandpharmacologicalrescueofionchanneldiseasesenabledbyimprovedcardiacinductionofhumanpluripotentstemcellsin2Dand3Dformats Boris Greber;MaxPlankInstituteforMolecularBiomedicine,Münster


Room K 1+2: Pluripotency and embryonic stem cells (chair: James Adjaye / Mathias Treier)09:00–09:20 Pluripotencyandembryonicstemcells:Introductionandoverview

James Adjaye; UniversityHospitalofDüsseldorf09:20–09:35 T22-Primate-specificendogenousretrovirusdriventranscriptiondefines

naïve-like stem cells Jichang Wang;MaxDelbrückCenter,Berlin

09:35–09:50 T23-AnovellncRNA-proteininteractioncharacterizesmouseembryonicstemcell fate Debojyoti Chakraborty;DresdenUniversityofTechnology

09:50–10:05 T24-NANOGandCDX2patterndistinctsubtypesofhumanmesodermduringexitfrompluripotency Sasha Mendjan;CambridgeStemCellInstitute,UnitedKingdom

10:05–10:20 T25-Insightsfromthestreak:identificationofnovelgenesassociatedwithgerm-layerformationandmesendodermdifferentiation Cantas Alev;RIKENCenterforDevelopmentalBiology,Kobe,Japan


ZMBH(groundfloor): Stem cells in development (chair: Jan Lohmann / Francesca Spagnoli)09:00–09:15 Stemcellsindevelopment–Overview

Jan Lohmann; Centre for Organismal Studies Heidelberg 09:15–09:30 T26-Tet-dependentprocessingof5-methylcytosineprotectsDNAmethylation

canyonsagainsthypermethylation Achim Breiling;GermanCancerResearchCenter,Heidelberg

Conferenceprogram


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09:30–09:45 T27-PIEZO2isrequiredformechanotransductioninhumanES-cell-derivedtouchreceptors Katrin Schrenk-Siemens;UniversityofHeidelberg

09:45–10:00 T28-Transcriptionalandepigeneticdynamicsunderlyingcelllineagecommitment Filippo M. Cernilogar;LudwigMaximilianUniversity,Munich

10:00–10:15 T29-Thehistone3lysine4methyltransferaseMll2primestheNxt2promoterforneuraldifferentiation Katrin Neumann;DresdenUniversityofTechnology

10:15–10:30 T30-Signalsfromtheinjurynicheincreasefatepotentialofadultneuralstemcells Enric Llorens Bobadilla;GermanCancerResearchCenter,Heidelberg


Industry session: “Technologies from GSCN industry partners”Lecture hall: Main supporters11:15–11:20 Introduction(Andreas Trumpp)11:20–11:50 C01-HighthroughputscreeningusingiPSC-basedmodels

Jared Sterneckert;CRTDresden,representingPeproTechGmbH11:50–12:20 C02-Controllingexpansionandcardiomyogenicdifferentiationofhuman

pluripotentstemcellsinscalablesuspensionculture Robert Zweigerdt;MHHannover,representingEppendorfAG

12:20–12:50 C03-Rawmaterialsinthemanufactureofadvancedtherapiesmedicinal products:Qualityattributesandqualityassurance Bernd Leistler; CellGenix GmbH

Room K 1+2: Supporters11:15–11:20 Introduction(Michael Cross)11:20–11:50 C04–EfficientneuralandcardiacdifferentiationsystemsforiPSC

Mohan C. Vemuri;LifeTechnologiesGmbH11:50–12:20 C05-Cell-basedgenedeliveryleveragesconventionalimmunotherapyfor

cancer;TreatmentofadvancedgastrointestinalcancerinaphaseI/IItrialwithmodifiedautologousMSC_apceth_101 Volker Scherhammer;apcethGmbH&Co.KG

12:20–12:50 C06-Scalableenzyme-freeprotocolsfortheisolationandmaintenanceof humaninducedpluripotentstemcellswithoutmechanicalcolonyscraping Alexandra Blak;STEMCELLTechnologiesSARL

ZMBH(groundfloor): Supporters11:15–11:20 Introduction(Torsten Tonn)11:20–11:50 C07-Thefutureiscounting–DigitalanalysisofRNA,DNAandproteinbiomarkers

Maik Pruess;NanoStringTechnologiesGermanyGmbH11:50–12:20 C08–PrimateiPScellsastoolsforevolutionaryanalyses

Wolfgang Enard;LudwigMaximilliansUniversity,Munich;representing FluidigmEuropeB.V.


12:20–12:50 C09–Buildingbridgesfromresearchtotherapy:Aroadmapforthesuccessfulgenerationofclinical-gradeiPSCs Thomas Fellner;LonzaCologneGmbH

12:50–14:30 Lunchbuffet / industryexhibition / posterviewing supported by STEMCELL Technologies SARL

13:15–14:15 Room K 1+2: Explainingscienceandmotivatingpeople–Outreachmaterialsfor stem cell research (Tobias Cantz / Ira Herrmann)

Concurrent strategic working group session ALecture hall: Career development (chairs: Hartmut Geiger / Insa Schröder)14:30–14:45 Careersinindustry, Andreas Bosio; MiltenyiGmbH,Berg.Gladbach14:45–15:00 Careersinscienceadministration,Hella Lichtenberg; DLRBonn15:00–15:15 Careersinacademia,Hartmut Geiger / Insa Schröder15:15–15:30 Paneldiscussion

Room K 1+2: Clinical trials and regulatory affairs I (chair: Andreas Kurtz / Torsten Tonn / Hans-Dieter Volk) supported by apceth GmbH & Co� KG

14:30–14:50 DevelopmentofiPScell-basedcardiactherapy Ulrich Martin, MH Hannover

14:50–15:10 Regulatorychallengesofstemcell-basedmedicinalproducts Egbert Flory;PaulEhrlichInstitute,Langen


ZMBH(groundfloor): Large scale banking of human pluripotent stem cells – workshop (chair: Andreas Kurtz / Joana Namorado)

14:30–14:45 BiomedicalapplicationsofiPScelltechnology Angel Raya; CMRB Barcelona

14:45–15:00 Theneedforlarge-scaleiPSCcollections-apharmaperspective Alex Gutteridge;Pfizer

15:00–15:15 Deliveryofhumanpluripotentstemcellsfortherapy:PhaseIVoftheUKStemCellBankProject Lyn Healy;UKStemCellBank,SouthMimms,U.K.

15:15–15:30 Therelaunchofthehumanpluripotentstemcellregistry(hESCreg) Andreas Kurtz;BCRTBerlin

15:30–15:45 Shortbreak

Concurrent strategic working group session BLecture hall: Stem cell technologies (chair: Andreas Bosio / Frank Emmrich)15:45–15:50 Introduction:Andreas Bosio,MiltenyiGmbH,Berg.Gladbach15:50–16:05 Methodsofgeneticengineering

Claudio Mussolino; UniversityofFreiburg

Conferenceprogram


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16:05–16:20 Reprogrammingtechnologies Micha Drukker;HelmholtzCenterMunich

16:20–16:35 Methodsoflasercellprinting Boris Chichkov; LaserCenterHannover(LZH)


Room K 1+2: Clinical trials and regulatory affairs II (chair: Andreas Kurtz / Torsten Tonn / Hans-Dieter Volk)

15:45–16:05 Placentaexpandedmesenchymal(PLX)cells–preclinicaldataforpreparingclinical trials Lena Pinzur;PluriStemTherapeuticsInc.,Haifa,Israel

16:05–16:25 PLXcells–frompreclinicaltoclinicalstudies–whatdidwelearnsofarfrombiomarkerstudies? Hans-Dieter Volk;Berlin-BrandenburgCenterforReg.Therapies(BCRT)

16:25–16:45 Paneldiscussions

ZMBH(groundfloor): Funding opportunities for young and advanced scientists (chair: Hartmut Geiger / Albrecht Müller)

15:45–16:30 FundingopportunitiesoftheDFGforstemcellscientistsatvariouscareerstages Eckard Picht;GermanResearchFoundation(DFG),Bonn



16:45–17:45 DKFZ Mensa:Meet-the-experttable:Stem cell technologies – genetic engineering (Claudio Mussolino) limited to 10 registered participants

17:15–19:15 Poster session II: P098 – P187 (posterswillbedisplayedinthetent) P098–P129:Stemcellsinregenerativetherapies P130–P161:Stemcellsindiseasemodelinganddrugdevelopment (posterswillbedisplayedinfrontofroomK1+2) P162–P178:Stemcellsindisease:Cancerstemcells P179–P187:Computationalstemcellbiology

Evennumbers:pleasepresentyourposterfrom17:15–18:15 Unevennumbers:18:15–19:15

19:15–20:00 FreebusshuttletoNetworkingEvening (busseswilldepartfromtheDKFZmainentrance)

20:00–01:00 NetworkingEveningwithdinnerbuffetatSchlosshotel“Molkenkur” Klingenteichstr.31,69117Heidelberg(www.molkenkur.de) (Opening:Oliver Brüstle)(includedintheregistrationfee) supported by CellGenix GmbH


WEDNESDAY, November 5th

Concurrent scientific working group session IIILecture hall: Hematopoietic stem cells (chair: Anthony Ho / Claudia Waskow)09:00–09:20 Introduction“Hematopoieticstemcells”

Claudia Waskow;TechnicalUniversityDresden09:20–09:35 T31-Stroma-derivedosteopontinregulateshematopoieticstemand

progenitorcellsfunctionuponaging Novella Guidi;UniversityofUlm

09:35–09:50 T32-IdentificationofregulatorynetworksinHSCsandtheirimmediate progenyviaintegratedproteome,transcriptome,andDNAmethylomeanalysis Daniel Klimmeck;GermanCancerResearchCenter,Heidelberg

09:50–10:05 T33-Kitdeficiencyregulatesstablehumanhematopoieticstemcell engraftmentinmice Susann Rahmig;TechnicalUniversityDresden

10:05–10:20 T34-STAT5-regulatedmiRNA193bcontrolshematopoieticstemandprogenitorcellexpansionbyfinetuningcytokinesignaling Nadine Hätscher;UniversityHospitalFrankfurt


Room K 1+2: Programming and reprogramming (chair: Micha Drukker / Frank Edenhofer)09:00–09:15 Introductiontore-programming

Frank Edenhofer;UniversityofWürzburg09:15–09:30 T35-SelectivegenedeliveryintohumaniPScellsbasedonCD30-targeted

lentiviralvectors Thorsten Friedel;PaulEhrlichInstitute,Langen

09:30–09:45 T36-GenerationofiPScellsfromyoungvs.agedcellsources–influenceofdonorageonreprogrammingefficiencyandgeneticstabilityofiPSclones Katarzyna Osetek;LEBAO / HannoverMedicalSchool

09:45–10:00 T37-MesenchymalstemcellsderivedfromiPScellsfromagedindividuals acquirefetalcharacteristics Lucas Spitzhorn; UniversityHospitalofDüsseldorf

10:00–10:15 T38-TRIM32,adualplayerregulatingtheentryandexitfrompluripotency Lamia’a Bahnassawy;UniversityofLuxembourg

10:15–10:30 T39-RobustgenerationofcardiomyocytesfromhumaniPScellsrequires precisemodulationofBmpandWntsignaling Asifiqbal Kadari;JuliusMaximiliansUniversity,Würzburg

DKFZ SR 4th floor: Stem cells in regenerative therapies II (chair: Michael Cross / Frank Emmrich)H2.04.073

The meeting point for this session is at the registration desk!09:00–09:15 IntroductiontosessionIIstemcellsinregenerativetherapies

Michael Cross;UniversityofLeipzig

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09:15–09:30 T40-EfficientgenerationoffunctionallydefinediPSC-derivedhumanneuralstem/progenitorcellsandoligodendrocytes Giacomo Frati;SanRaffaeleScientificInstitute,Milan,Italy

09:30–09:45 T41-Agingaffectsthemesenchymalstemcellderivedoligodendrogenic / remyelinationactivities:implicationforapotentialtherapyofmultiplesclerosis Francisco J. Rivera;ParacelsusMedicalUniversity,Salzburg,Austria

09:45–10:00 T42-TheeuropeanprojectREBORNE:Multipotentmesenchymalstromalcellsand biomaterial for bone healing Alexander Erle;UlmUniversityHospital

10:00–10:15 T43-Identification,isolationandtargetedinhibitionofafibroblastlineageresponsibleforscarringandcancerstroma Yuval Rinkevich;StanfordInstituteforStemCellBiologyandRegenerativeMedicine,U.S.A.

10:15–10:30 T44-Proofingthe(Un)Proven:Provenorapprovedstemcelltherapies– thatisthequestion Bianca Büchner;CenterforEthicsandLawintheLifeSciences(CELLS),Hannover


Concurrent workshopsLecture hall: Clinical relevance of animal models in stem cell research and regenerative

medicine (chair: Georg Duda / Frank Emmrich) (Workshopofthe‘RegenerativeMedicineInitiativeGermany(RMIG)’)

11:00–11:20 Introduction:Animalmodelsinresearchforregenerativemedicine Axel Haverich;Rebirth / HannoverMedicalSchool

11:20–11:30 Celltransplantationintopre-clinicalmodelsofretinaldegeneration Marius Ader; CenterforRegenerativeTherapiesDresden

11:30–11:40 Medicaldevicesandtheapplicationofstemcells Konrad Kohler;zrm,TübingenUniversityHospital

11:40–11:50 Regenerativemedicineforanagingsociety:Howrelevantareourcurrent animalmodels? Georg Duda;BCRTBerlin

11:50–12:00 Heterogeneity,age,comorbiditiesandthetranslationalfailure–canwedobetterinprojectingfrompreclinicalstemcellstudiestoclinicaltrials? Johannes Boltze;TRMLeipzig

12:00–12:10 Hurdlesforclinicaltranslationofregenerativestemcelltherapy– lessonslearnedfromthecardiacPhaseIIIPERFECTmulticentertrial Gustav Steinhoff;RTCRostock

12:10–12:30 Paneldiscussion

Room K 1+2: Big pharma and the stem cell field – matching expectations? (organizer:Oliver Brüstle / Ira Herrmann) (WorkshopoftheStemCellNetworkNorthRhineWestphalia)


Panel: Oliver Brüstle;LIFE&BRAINGmbH,Bonn Ralf Heilker; BoehringerIngelheimPharmaGmbH&Co.KG,Div.ResearchGermany Jörg Hüser;BayerPharmaAG,LeadDiscoveryWuppertal Bert Klebl;LeadDiscoveryCenterGmbH,Dortmund Moderation:Ira Herrmann;StemCellNetworkNRW,Düsseldorf

ZMBH(groundfloor): Proceedings of the German Society for Stem Cell Research (GSZ e.V.) (chair: Jürgen Hescheler)

11:00–11:10 Introduction11:10–11:30 Sinusnodalcellsfrompluripotentstemcells

Robert David;ReferenceandTranslationCenterforCardiacStemCell Therapy(RTC),Rostock

11:30–11:50 Chondrogeniclineagedeterminationduringdifferentiationofmouseembryonicstem cells Jürgen Rohwedel;UniversityofLübeck

11:50–12:10 UseoftranscriptomicsandepigeneticapproachesinhumanESC-based developmentalneurotoxicology(DNT)testing Tanja Waldmann;UniversityofKonstanz

12:10–12:30 LongtermcultureofiPSC-derivedcardiomyocytesonmechanically-definedsubstrates Carlos Heras;UniversityofCologne

12:30–12:45 Shortbreak

Lecture hall:12:45–13:30 Keynote lecture III

K3-Molecularandcellularregulationofmusclestemcellsduringdevelopmentandregeneration Shahragim Tajbakhsh;InstitutePasteur,Paris,France (Chair: Oliver Brüstle)

13:30–15:00 Lunchbuffet / industryexhibition supported by Nanostring Technologies Germany GmbH

13:45–14:45 Room K 1+2:Explainingscienceandmotivatingpeople–Outreachmaterialsforstem cell research (Tobias Cantz / Ira Herrmann)

13:45–14:45 DKFZ Mensa:Meet-the-experttable:Stem cell technologies – laser printing (Boris Chichkov)and– reprogramming technologies (Micha Drukker) limited to 10 registered participants each

Closing ceremonyLecture hall:15:00–15:15 Posterawardceremoniesandannouncementofindustryquizwinners

(Daniel Besser; GSCN) supported by PeproTech GmbH

15:15–15:30 Outlookandclosingremarks Incomingpresident:Thomas Braun

Conferenceprogram

2nd International Annual Conference of the GSCN

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15:30–16:15 Keynote lecture IV – Joined session with the 6th BMBF symposium – Adult neural stem cells (Chair: Magdalena Götz / Ana Martin-Villalba) K4-Modelinghumanbraindevelopmentanddiseasein3Dculture Jürgen Knoblich;InstituteofMolecularBiotechnology(IMBA) Vienna,Austria supported by Life Technologies GmbH

16:15–17:00 K5-Adulthippocampalneurogenesis:Whatarenewneuronsgoodfor? Andwhatisgoodforthem? Amelia Eisch;UTSouthwestern,Dallas,U.S.A.

17:00–17:45 K6-Amechanismforthesegregationofageinmammalianneuralstemcells Sebastian Jessberger; UniversityofZurich,Switzerland

End of GSCN conference

Announcements:18:30–20:00 PublicoutreacheventoftheGSCNincollaborationwiththeScheringStiftung

andtheDKFZ(inGerman) Stammzellen als Chance – Realität und Perspektiven PodiumsdiskussionzuraktuellenForschunginLaborundKlinik

Panel: Magdalena Götz(LMUMünchen),Anthony Ho(Uni.Heidelberg), Andreas Trumpp(DKFZ) Moderation:Stefanie Seltmann(DKFZ)

20:00–21:00 Receptionpublicoutreachevent

and

November 6th, 2014 – GSCN Satellite Event: 6th BMBF Symposium – Adult neural stem cellsTheprogramofthesatelliteeventisavailableatthereceptiondesk.

Supported by:

Conferenceprogram

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