operation debug - main page -...
TRANSCRIPT
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Operation Debug
NCTU_Formosa
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Harmful Insect Elimination Force
Headquarters: National Chiao Tung University, Hsinchu, Taiwan
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Insect Damage
Current Solution: Pesticide
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Motivation|
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Target |
Oriental Leafworm Moth
(Spodoptera litura)
Agricultural damage to
over 40 families of plants
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Tactical
Plan A : Biologically Synthesized Pheromone Traps
Plan |
5
Regulatory Role of PBAN in Sex Pheromone Biosynthesis of Heliothine Moths ( Russell Jurenka, and Ada Rafaeli, Oct 10 2011 )
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Tactical
Plan B :
Plan |
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PBAN(Pheromone Biosynthesis Activating Neuropeptide)
Infiltration
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Tactical Plan |
Gland
Receptor
PBAN
Pheromone
Biosynthesis Activated
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PBAN
Specialties
(1) Peptide
(2) Small
(3) Stable
(4) Specific
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Operation Detail |
Pheromone
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Biobrick
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Biobrick |
RBS PBAN Ter
Pcons
PBANPBAN-SL
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Results – Making PBAN|
Oriental Leafworm Moth
(Spodoptera litura)
PCR
750 bp500 bp
SDS-PAGEControl
25 kDa
11 kDa
3.7 kDa
BBa_K1415105
11
RBS PBAN-SL
Pcons
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Results-Making PBAN|
BBa_K1415205
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PBAN-SL Pcons
BFPRBS RBS Ter
Control PBAN-SL
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Ca2+
Adenylate Cyclase
PBAN receptorCalmodulin
PBAN
G protein
Mechanism |
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CentrifugeAdd
Buffer
Pour out
LB
Vortex
MashDilute
Autoclave
Obtain Bio-safe PBAN solution
E.coli contain PBAN
Sonicator
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PBAN
Cotton
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Control
( Without PBAN )With PBAN
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• |
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Parts of Device |
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Operational Process|
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Operational Process |
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Modeling|
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Device for light preference
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Result - PCR |
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SL
BM MB AI
LD HAH
AS SI AA
750 bp
MB AI LD SL HAH AS SI AA
500 bp
250 bp
BM
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Result - SDS PAGE |
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Control BM AA LD AS
25 kDa
11 kDa
AI MB HAH SIControl
2-4 kDa
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Control
SLBM
MBAI LD
HAHAS
SI
AA
PBAN-SL Pcons
BFPRBS RBS Ter
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Human Practice
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Cooperation |
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Industry Collaboration
Team ExchangeiGEM Promoting
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Organic Farm |
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iGEM Conference |
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Bio-camp|
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Green Market |
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Achievements|
New basic and composite BioBrick parts
We converted 9 PBANs into standard parts and constructed 27 new BioBricks.
Successfully resolving insect damage
We developed a brand new control system and a device for its application.
Real application in industry
Organic farms are currently using our new system and loving it.
Team Meetups
We held an international conference with 22 iGEM teams from China and Taiwan.
Help any registered iGEM team from another school
We assisted TCU Taiwan iGEM Team with their modeling.
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Acknowledgement
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Instructors:
Prof. Yan-Hwa Wu
Prof. Yuh-Jyh Jong
Prof. Hsien-Da Huang
Prof. Shin-Ying Ho
Prof. Hsiao-Ching Lee
Prof. Wen-Liang Chen
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Mission
Accomplished