446 ASSOCIATION FOR ACADEMIC SURGERY AND SOCIETY OF UNIVERSITY SURGEONS—ABSTRACTS

ior is aggregated into population behavior that mirrors the behaviorof the higher-hierarchical system as a whole. Herein are presented aseries of ABMs of inflammation developed at multiple levels of res-olution leading up to simulated organ function and organ-organinteractions. These levels include: 1) ABMs of intracellular signalingin response to inflammatory stimuli, 2) ABMs matching in-vitro cellculture response to inflammatory cytokines, 3) ABMs of different celltypes aggregated to simulate tissue/whole organ behavior, 4) ABMslinked to simulate an organ-organ axis of pathophysiology (the gut-lung axis of injury) and finally 5) an overall modeling architecturethat incorporates organ-organ crosstalk and multiple tissues andorgans to create a “virtual patient.” These virtual patients will formthe basis of simulated in-silico clinical trials that will be the vitalcomponent in the future of translational drug design and the devel-opment of personalized medicine.

QS449. HAND-HELD PET PROBES AS A TOOL FOR INTRA-OPERATIVE LOCALIZATION OF CANCER. Vivian E.Strong, Charles Galanis, Christopher Riedl, Valerie Longo,John Humm, Steven Larson, Yuman Fong; Memorial Sloan-Kettering Cancer Center, New York, NY

Introduction: Positron Emission Tomography (PET) detects re-lease of gamma emissions to aid diagnosis and evaluation of cancer.However, correlating preoperative PET images with intraoperativefindings remains a challenge and the smallest deposits detectableare 1cm, making detection of metastatic deposits challenging.Positron emitters used for PET not only release long-range gammaemissions, but also short-range beta emissions that can be detectedwith a novel hand-held probe. We investigated whether beta emis-sions detected by hand-held portable PET probes would correlatewith conventionally detected gamma emission and offer a novel wayto better localize tumor intraoperatively. Methods: Beta and gammaemissions were correlated in multiple murine tumor models (breast,gastric, squamous cell, and pancreatic cancer). Mice (n�10) wereinjected with 4 microCu 18-Fluorodeoxyglucose and underwentmicro-PET imaging. Portable probe measurements of gamma andbeta emission from tumor and normal tissue were made in vitro, invivo, and ex vivo. Tissues were excised, weighed, and cut for auto-radiography and hematoxylin and eosin staining. Results: The por-table PET probe detected beta and gamma emission from all tumors.Areas of detection corresponded to regions of intensity on PET im-aging and to areas positive for tumor by H�E staining. The smallestdeposits found in vivo were 4mm in size. Additionally, gamma andbeta emission exhibited a strong positive correlation (R�0.8).

Beta emission showed a stronger correlation than gamma emissionwith overall tissue radioactivity. Conclusion(s): This study demon-strates that gamma emission detected by conventional PET imagingcorrelates directly with beta emission. Further, this study suggeststhat compared to detection of gamma emission, beta emission detec-

tion can offer superior real-time localization of tumor by detecting

smaller deposits than possible with gamma emissions. Intraopera-tive portable PET probe may become a useful way to exploit tumorbiology and PET technology to guide surgical therapy.

QS450. IMPROVED ASSESSMENT OF MELANOMA MARGINUSING ULTRAVIOLET LIGHT. Christopher R. Rouse,Summer R. Youker, Marie Y. Hurley, Cherise M. Cortese,Scott W. Fosko, Eddy C. Hsueh, Frank E. Johnson; SaintLouis University, St. Louis, MO

Introduction: Judging the border of a primary melanoma affects theplan of excision. Currently this relies on examination using visible light.Ultraviolet light (UV) can alter the apparent border, but the outcomesof UV-aided margin assessment have not been reported. Methods: Insitu and invasive primary melanoma lesions were evaluated with bothvisible light and UV, using a hand-held Wood’s lamp. Shallow incisionswere made at the visible border of the lesion using each type of light.The visible-light margin and the UV margin were marked with differ-ent ink colors if they were not identical. The lesions were excised usingthe visible-light margin as a reference. Microscopic margins were eval-uated via standard Methods. The visible-light margin and the UVmargin were compared. The distances between the microscopic marginand both visible macroscopic margins were measured using an ocularmicrometer. Results: 27 lesions were examined; 17 enhanced beyondthe visible-light border with UV. 12 slides from10 lesions were evalu-able, due to early learning-curve difficulties. The maximum distancebetween the UV and visible-light margins was 4.6 � 2.9 mm (mean �SD). In 8 of the 12 slides, the UV margin was closer than the visible-light margin to the microscopic margin; in 4, the visible-light marginwas closer than the UV margin. The distance from the visible-lightmargin to the microscopic margin was 3.6 � 4.3 mm (mean � SD). Thedistance from the UV margin to the microscopic margin was 0.2 � 4.5mm. Conclusions: The UV margin extended past the visible-lightmargin in 17/27 (63%) of lesions. The microscopic margin was moreclosely approximated by the UV margin in 8/12 (67%) and by thevisible-light margin in 4/12 (33%). UV methodology is simple to use,quick, reproducible, and inexpensive. Improving the surgeon’s ability tojudge the microscopic margin by UV might permit a decrease in theallowable margin, possibly improving cosmesis without compromisingcure rates.

SUS NEW MEMBER POSTERS A

P1. BLOOD PRODUCT TRANSFUSIONS IN PEDIATRICTRAUMA PATIENTS ARE NOT ASSOCIATED WITH AN IN-CREASE IN MORBIDITY AND MORTALITY. SUS NEW MEM-BER POSTER SESSION: THU 2/14 4:30 PM. Cynthia Leaphart,Megan Sippey, Kathy Gismondi, Barbara A. Gaines; Children’s Hos-pital of Pittsburgh, Pittsburgh, PA

Abstract not available

P2. THE BUSINESS PRACTICES IN AN ACADEMIC DE-PARTMENT OF SURGERY: CAN WE SURVIVE? C. DanielSmith; Mayo Medical School, Jacksonvile, FL

Abstract not available

P3. OPEN VERSUS LAPAROSCOPIC PYLOROMYOTOMYFOR PYLORIC STENOSIS: A PROSPECTIVE RANDOMIZEDCONTROLLED TRIAL. Shawn D. St Peter, Sr., George W. Hol-comb III, Casey M. Calkins, Sr., John P. Murphy, Sr., Walter S.Andrews, Sr., Ronald J. Sharp, Sr., Charles L. Snyder, Sr., Daniel J.Ostlie; Children’s Mercy Hospitals and Clinics, Kansas City, MO

Abstract not available