Package ‘biomaRt’June 20, 2018

Version 2.36.1

Title Interface to BioMart databases (e.g. Ensembl, COSMIC, Wormbaseand Gramene)

Depends methods

Imports utils, XML, RCurl, AnnotationDbi, progress, stringr, httr

Suggests annotate, BiocStyle, knitr, rmarkdown, testthat

VignetteBuilder knitr

biocViews Annotation

Description In recent years a wealth of biological data has become availablein public data repositories. Easy access to these valuable dataresources and firm integration with data analysis is needed forcomprehensive bioinformatics data analysis. biomaRt provides aninterface to a growing collection of databases implementing theBioMart software suite (http://www.biomart.org). The package enablesretrieval of large amounts of data in a uniform way without the need toknow the underlying database schemas or write complex SQL queries.Examples of BioMart databases are Ensembl, COSMIC, Uniprot, HGNC,Gramene, Wormbase and dbSNP mapped to Ensembl. These major databasesgive biomaRt users direct access to a diverse set of data and enablea wide range of powerful online queries from gene annotation todatabase mining.

License Artistic-2.0

LazyLoad yes

NeedsCompilation no

git_url https://git.bioconductor.org/packages/biomaRt

git_branch RELEASE_3_7

git_last_commit 5634e57

git_last_commit_date 2018-05-23

Date/Publication 2018-06-20

Author Steffen Durinck [aut],Wolfgang Huber [aut],Sean Davis [ctb],Francois Pepin [ctb],Vince S Buffalo [ctb],Mike Smith [ctb, cre]

Maintainer Mike Smith <grimbough@gmail.com>

1

2 attributePages

R topics documented:

attributePages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2exportFASTA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3filterOptions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3filterType . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4getBM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5getBMlist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6getGene . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7getLDS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8getSequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9getXML . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10listAttributes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11listDatasets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12listEnsembl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13listEnsemblArchives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13listFilters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14listMarts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15Mart-class . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16NP2009code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16select-methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17useDataset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18useEnsembl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19useMart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Index 21

attributePages Gives a summary of the attribute pages

Description

Attributes in BioMart databases are grouped together in attribute pages. The attributePages functiongives a summary of the attribute categories and groups present in the BioMart. These page namescan be used to display only a subset of the available attributes in the listAttributes function.

Usage

attributePages(mart)

Arguments

mart object of class Mart, created with the useMart function.

Author(s)

Steffen Durinck

exportFASTA 3

Examples

if(interactive()){mart = useMart("ensembl", dataset="hsapiens_gene_ensembl")attributeSummary(mart)}

exportFASTA Exports getSequence results to FASTA format

Description

Exports getSequence results to FASTA format

Usage

exportFASTA(sequences, file)

Arguments

sequences A data.frame that was the output of the getSequence function

file File to which you want to write the data

Author(s)

Steffen Durinck

Examples

if(interactive()){mart <- useMart("ensembl", dataset="hsapiens_gene_ensembl")

#seq<-getSequence(chromosome=c(2,2),start=c(100000,30000),end=c(100300,30500),mart=mart)#exportFASTA(seq,file="test.fasta")

martDisconnect(mart = mart)}

filterOptions Displays the filter options

Description

Displays a set of predetermed values for the specified filter (if available).

Usage

filterOptions(filter,mart)

4 filterType

Arguments

filter A valid filter name.

mart object of class Mar created using the useMart functiont

Author(s)

Steffen Durinck

Examples

if(interactive()){mart = useMart("ensembl", dataset="hsapiens_gene_ensembl")filterOptions("chromosome_name", mart)}

filterType Displays the filter type

Description

Displays the type of the filer given a filter name.

Usage

filterType(filter,mart)

Arguments

filter A valid filter name. Valid filters are given by the listFilters function

mart object of class Mart, created using the useMart function

Author(s)

Steffen Durinck

Examples

if(interactive()){mart = useMart("ensembl", dataset="hsapiens_gene_ensembl")filterType("chromosome_name", mart)}

getBM 5

getBM Retrieves information from the BioMart database

Description

This function is the main biomaRt query function. Given a set of filters and corresponding values,it retrieves the user specified attributes from the BioMart database one is connected to.

Usage

getBM(attributes, filters = "", values = "", mart, curl = NULL,checkFilters = TRUE, verbose = FALSE, uniqueRows = TRUE, bmHeader = FALSE,quote = "\"")

Arguments

attributes Attributes you want to retrieve. A possible list of attributes can be retrievedusing the function listAttributes.

filters Filters (one or more) that should be used in the query. A possible list of filterscan be retrieved using the function listFilters.

values Values of the filter, e.g. vector of affy IDs. If multiple filters are specified thenthe argument should be a list of vectors of which the position of each vectorcorresponds to the position of the filters in the filters argument.

mart object of class Mart, created with the useMart function.

curl An optional ’CURLHandle’ object, that can be used to speed up getBM whenused in a loop.

checkFilters Sometimes attributes where a value needs to be specified, for example upstream\_flankwith value 20 for obtaining upstream sequence flank regions of length 20bp, aretreated as filters in BioMarts. To enable such a query to work, one must specifythe attribute as a filter and set checkFilters = FALSE for the query to work.

verbose When using biomaRt in webservice mode and setting verbose to TRUE, theXML query to the webservice will be printed.

uniqueRows If the result of a query contains multiple identical rows, setting this argument toTRUE (default) will result in deleting the duplicated rows in the query result atthe server side.

bmHeader Boolean to indicate if the result retrieved from the BioMart server should includethe data headers or not, defaults to FALSE. This should only be switched on ifthe default behavior results in errors, setting to on might still be able to retrieveyour data in that case

quote Sometimes parsing of the results fails due to errors in the Ensembl data fieldssuch as containing a quote, in such cases you can try to change the value ofquote to try to still parse the results.

Value

A data.frame. There is no implicit mapping between its rows and the function arguments (e.g.filters, values), therefore make sure to have the relevant identifier(s) returned by specifyingthem in attributes. See Examples.

6 getBMlist

Author(s)

Steffen Durinck

Examples

mart <- useMart(biomart = "ensembl", dataset = "hsapiens_gene_ensembl")getBM(attributes = c("affy_hg_u95av2", "hgnc_symbol", "chromosome_name", "band"),

filters = "affy_hg_u95av2",values = c("1939_at","1503_at","1454_at"),mart = mart)

getBMlist Retrieves information from the BioMart database

Description

This function is the main biomaRt query function. Given a set of filters and corresponding values,it retrieves the user specified attributes from the BioMart database one is connected to

Usage

getBMlist(attributes, filters = "", values = "", mart,list.names = NULL, na.value = NA, verbose = FALSE, giveWarning = TRUE)

Arguments

attributes Attributes you want to retrieve. A possible list of attributes can be retrievedusing the function listAttributes.

filters Filters (one or more) that should be used in the query. A possible list of filterscan be retrieved using the function listFilters.

values Values of the filter, e.g. vector of affy IDs. If multiple filters are specified thenthe argument should be a list of vectors of which the position of each vectorcorresponds to the position of the filters in the filters argument.

mart object of class Mart, created with the useMart function.list.names names for objects in listna.value value to give when result is emptyverbose When using biomaRt in webservice mode and setting verbose to TRUE, the

XML query to the webservice will be printed.giveWarning Gives a warning about best practices of biomaRt and recommends using getBM

instead of getBMlist

Author(s)

Steffen Durinck

Examples

if(interactive()){mart <- useMart("ensembl")datasets <- listDatasets(mart)

}

getGene 7

getGene Retrieves gene annotation information given a vector of identifiers

Description

This function retrieves gene annotations from Ensembl given a vector of identifiers. Annotationincludes chromsome name, band, start position, end position, gene description and gene symbol. Awide variety of identifiers is available in Ensembl, these can be found with the listFilters function.

Usage

getGene( id, type, mart)

Arguments

id vector of gene identifiers one wants to annotate

type type of identifier, possible values can be obtained by the listFilters function. Ex-amples are entrezgene, hgnc\_symbol (for hugo gene symbol), ensembl\_gene\_id,unigene, agilentprobe, affy\_hg\_u133\_plus\_2, refseq\_dna, etc.

mart object of class Mart, containing connections to the BioMart databases. You cancreate such an object using the function useMart.

Author(s)

Steffen Durinck

Examples

if(interactive()){

mart = useMart("ensembl", dataset="hsapiens_gene_ensembl")

#example using affy id

g = getGene( id = "1939_at", type = "affy_hg_u95av2", mart = mart)show(g)

#example using Entrez Gene id

g = getGene( id = "100", type = "entrezgene", mart = mart)show(g)}

8 getLDS

getLDS Retrieves information from two linked datasets

Description

This function is the main biomaRt query function that links 2 datasets and retrieves informationfrom these linked BioMart datasets. In Ensembl this translates to homology mapping.

Usage

getLDS(attributes, filters = "", values = "", mart, attributesL,filtersL = "", valuesL = "", martL, verbose = FALSE, uniqueRows = TRUE,bmHeader=TRUE)

Arguments

attributes Attributes you want to retrieve of primary dataset. A possible list of attributescan be retrieved using the function listAttributes.

filters Filters that should be used in the query. These filters will be applied to primarydataset. A possible list of filters can be retrieved using the function listFilters.

values Values of the filter, e.g. list of affy IDs

mart object of class Mart created with the useMart function.

attributesL Attributes of linked dataset that needs to be retrieved

filtersL Filters to be applied to the linked dataset

valuesL Values for the linked dataset filters

martL Mart object representing linked dataset

verbose When using biomaRt in webservice mode and setting verbose to TRUE, theXML query to the webservice will be printed. Alternatively in MySQL modethe MySQL query will be printed.

uniqueRows Logical to indicate if the BioMart web service should return unique rows onlyor not. Has the value of either TRUE or FALSE

bmHeader Boolean to indicate if the result retrieved from the BioMart server should includethe data headers or not, defaults to TRUE. This should only be switched off ifthe default behavior results in errors, setting to off might still be able to retrieveyour data in that case

Author(s)

Steffen Durinck

Examples

if(interactive()){human = useMart("ensembl", dataset = "hsapiens_gene_ensembl")mouse = useMart("ensembl", dataset = "mmusculus_gene_ensembl")getLDS(attributes = c("hgnc_symbol","chromosome_name", "start_position"),

filters = "hgnc_symbol", values = "TP53", mart = human,attributesL = c("chromosome_name","start_position"), martL = mouse)

}

getSequence 9

getSequence Retrieves sequences

Description

This function retrieves sequences given the chomosome, start and end position or a list of identifiers.Using getSequence in web service mode (default) generates 5’ to 3’ sequences of the requested typeon the correct strand.

Usage

getSequence(chromosome, start, end, id, type, seqType,upstream, downstream, mart, verbose = FALSE)

Arguments

chromosome Chromosome name

start start position of sequence on chromosome

end end position of sequence on chromosome

id An identifier or vector of identifiers.

type The type of identifier used. Supported types are hugo, ensembl, embl, entrez-gene, refseq, ensemblTrans and unigene. Alternatively one can also use a filterto specify the type. Possible filters are given by the listFilters function

seqType Type of sequence that you want to retrieve. Allowed seqTypes are given in thedetails section.

upstream To add the upstream sequence of a specified number of basepairs to the output.

downstream To add the downstream sequence of a specified number of basepairs to the out-put.

mart object of class Mart created using the useMart function

verbose If verbose = TRUE then the XML query that was send to the webservice will bedisplayed.

Details

The type of sequence returned can be specified by the seqType argument which takes the followingvalues:

• ’cdna’: for nucleotide sequences

• ’peptide’: for protein sequences

• ’3utr’: for 3’ UTR sequences

• ’5utr’: for 5’ UTR sequences

• ’gene_exon’: for exon sequences only

• ’transcript_exon_intron’: gives the full unspliced transcript, that is exons + introns

• ’gene_exon_intron’ gives the exons + introns of a gene;’coding’ gives the coding sequenceonly

• ’coding_transcript_flank’: gives the flanking region of the transcript including the UTRs, thismust be accompanied with a given value for the upstream or downstream attribute

10 getXML

• ’coding_gene_flank’: gives the flanking region of the gene including the UTRs, this must beaccompanied with a given value for the upstream or downstream attribute

• ’transcript_flank’: gives the flanking region of the transcript exculding the UTRs, this must beaccompanied with a given value for the upstream or downstream attribute

• ’gene_flank’: gives the flanking region of the gene excluding the UTRs, this must be accom-panied with a given value for the upstream or downstream attribute

In MySQL mode the getSequence function is more limited and the sequence that is returned is the5’ to 3’+ strand of the genomic sequence, given a chromosome, as start and an end position. Soif the sequence of interest is the minus strand, one has to compute the reverse complement of theretrieved sequence, which can be done using functions provided in the matchprobes package. ThebiomaRt vignette contains more examples on how to use this function.

Author(s)

Steffen Durinck

Examples

if(interactive()){mart <- useMart("ensembl", dataset="hsapiens_gene_ensembl")

seq = getSequence(id = "BRCA1",type = "hgnc_symbol",seqType = "peptide",mart = mart)

show(seq)

seq = getSequence(id="1939_at",type="affy_hg_u95av2",seqType="gene_flank",upstream = 20,mart = mart)

show(seq)

}

getXML Retrieves information from the BioMart database using an XML query

Description

DEPRECATED! - This function is a low level query function bypassing lots of biomaRts internalcontrols. It allows for a direct XML query to a known BioMart webservice host.

Usage

getXML(host="http://www.ensembl.org/biomart/martservice?", xmlquery)

Arguments

host URL to BioMart webservice, is set to http://www.ensembl.org/biomart/martservice?by default

xmlquery XML query that needs to be send to the webservice

listAttributes 11

Details

This function has now been deprecated. Similar functionality can be achieved using the unexportedfunction ‘biomaRt:::.submitQueryXML. If you are still using this function and this affects yourwork, please contact the package maintainer at https://github.com/grimbough/biomaRt/issues

Author(s)

Steffen Durinck, Mike Smith

Examples

if(interactive()){xmlquery="<?xml version='1.0' encoding='UTF-8'?>

<!DOCTYPE Query><Query virtualSchemaName = 'default' uniqueRows = '1'

count = '0' datasetConfigVersion = '0.6'><Dataset name = 'hsapiens_gene_ensembl'>

<Attribute name = 'ensembl_gene_id'/><Filter name = 'chromosome_name' value = 'Y' />

</Dataset></Query>"

getXML(host = "www.ensembl.org/biomart/martservice?", xmlquery = xmlquery)}

listAttributes lists the attributes available in the selected dataset

Description

Attributes are the outputs of a biomaRt query, they are the information we want to retrieve. Forexample if we want to retrieve all entrez gene identifiers of genes located on chromosome X, en-trezgene will be the attribute we use in the query. The listAttributes function lists the availableattributes in the selected dataset

Usage

listAttributes(mart, page,what = c("name","description","page"))

Arguments

mart object of class Mart created using the useMart function

page Show only the attributes that belong to the specified attribute page.

what vector of types of information about the attributes that need to be displayed. Canhave values like name, description, fullDescription, page

Author(s)

Steffen Durinck

12 listDatasets

Examples

if(interactive()){ensembl = useMart("ensembl", dataset="hsapiens_gene_ensembl")listAttributes(ensembl)}

listDatasets lists the datasets available in the selected BioMart database

Description

Lists the datasets available in the selected BioMart database

Usage

listDatasets(mart, verbose = FALSE)

Arguments

mart object of class Mart created with the useMart function

verbose Give detailed output of what the method is doing, for debugging purposes

Author(s)

Steffen Durinck

Examples

if(interactive()){

#marts <- listMarts()#index<-grep("ensembl",marts)

#mart <- useMart(marts[index])

#listDatasets(mart = mart)

#martDisconnect(mart = mart)}

listEnsembl 13

listEnsembl lists the available BioMart databases hosted by Ensembl

Description

This function returns a list of BioMart databases hosted by Ensembl. To establish a connection usethe useMart function.

Usage

listEnsembl(mart = NULL,host = "www.ensembl.org", version = NULL,GRCh = NULL, mirror = NULL, verbose = FALSE)

Arguments

mart mart object created with the useEnsembl function. This is optional, as you usu-ally use listMarts to see which marts there are to connect to.

host Host to connect to if different then www.ensembl.org

version Ensembl version to connect to when wanting to connect to an archived Ensemblversion

GRCh GRCh version to connect to if not the current GRCh38, currently this can onlybe 37

mirror Specify an Ensembl mirror to connect to. The valid options here are ’www’,’uswest’, ’useast’, ’asia’. If no mirror is specified the primary site at www.ensembl.orgwill be used.

verbose Give detailed output of what the method is doing, for debugging purposes

Author(s)

Steffen Durinck

Examples

if(interactive()){listEnsembl()}

listEnsemblArchives Lists the available archived versions of Ensembl

Description

Returns a table containing the available archived versions of Ensembl, along with the dates theywere created and the URL used to access them.

Usage

listEnsemblArchives()

14 listFilters

Author(s)

Mike Smith

Examples

listEnsemblArchives()

listFilters lists the filters available in the selected dataset

Description

Filters are what we use as inputs for a biomaRt query. For example, if we want to retrieve allentrezgene identifiers on chromosome X, chromosome will be the filter, with corresponding valueX.

Usage

listFilters(mart, what = c("name", "description"))

Arguments

mart object of class Mart created using the useMart function

what character vector indicating what information to display about the available fil-ters. Valid values are name, description, options, fullDescription, filters,type, operation, filters8, filters9.

Author(s)

Steffen Durinck

Examples

if(interactive()){mart = useMart("ensembl", dataset="hsapiens_gene_ensembl")listFilters(mart)}

listMarts 15

listMarts lists the available BioMart databases

Description

This function returns a list of BioMart databases to which biomaRt can connect to. By default allpublic BioMart databases are displayed. To establish a connection use the useMart function.

Usage

listMarts(mart = NULL, host="www.ensembl.org", path="/biomart/martservice",port=80, includeHosts = FALSE, archive = FALSE, ssl.verifypeer = TRUE,ensemblRedirect = NULL, verbose = FALSE)

Arguments

mart mart object created with the useMart function. This is optional, as you usuallyuse listMarts to see which marts there are to connect to.

host Host to connect to. Defaults to www.ensembl.org

path path to martservice that should be pasted behind the host to get to web serviceURL

port port to use in HTTP communication

includeHosts boolean to indicate if function should return host of the BioMart databases

archive Boolean to indicate if you want to access archived versions of BioMart database.Note that this argument is now deprecated and will be removed in the future. Abetter alternative is to specify the url of the archived BioMart you want to access.For Ensembl you can view the list of archives using listEnsemblArchives

ssl.verifypeer Set SSL peer verification on or off. By default ssl.verifypeer is set to TRUEensemblRedirect

This argument has now been deprecated.

verbose Give detailed output of what the method is doing, for debugging purposes

Details

If you receive an error message saying ’Unexpected format to the list of available marts’, this is oftenbecause there is a problem with the BioMart server you are trying to connect to, and something otherthan the list of available marts is being returned - often some like a ’down for maintainance’ page.If you browse to the provided URL and find a page that starts with ’<MartRegistry>’ this is the cor-rect listing and you should report the issue on the Bioconductor support site: https://support.bioconductor.org

Author(s)

Steffen Durinck, Mike Smith

Examples

if(interactive()){listMarts()}

16 NP2009code

Mart-class Class Mart

Description

Represents a Mart class, containing connections to different BioMarts

Methods

show Print summary of the object

Author(s)

Steffen Durinck

NP2009code Display the analysis code from the 2009 Nature protocols paper

Description

This function opens an editor displaying the analysis code of the Nature Protocols 2009 paper

Usage

NP2009code()

Details

The edit function uses getOption("editor") to select the editor. Use, for instance, options(editor="emacs")to set another editor.

Author(s)

Steffen Durinck, Wolfgang Huber

See Also

edit

Examples

if(interactive()){NP2009code()}

select-methods 17

select-methods Retrieve information from the BioMart databases

Description

select, columns and keys are used together to extract data from a Mart object. These functionswork much the same as the classic biomaRt functions such as getBM etc. and are provide hereto make this easier for people who are comfortable using these methods from other Annotationpackages. Examples of other objects in other packages where you can use these methods include(but are not limited to): ChipDb, OrgDb GODb, InparanoidDb and ReactomeDb.

columns shows which kinds of data can be returned from the Mart object.

keytypes allows the user to discover which keytypes can be passed in to select or keys as thekeytype argument.

keys returns keys from the Mart of the type specified by it’s keytype argument.

select is meant to be used with these other methods and has arguments that take the kinds of valuesthat these other methods return. select will retrieve the results as a data.frame based on parametersfor selected keys and columns and keytype arguments.

Usage

columns(x)keytypes(x)keys(x, keytype, ...)select(x, keys, columns, keytype, ...)

Arguments

x the Mart object. The dataset of the Mart object must already be specified for allof these methods.

keys the keys to select records for from the database. Keys for some keytypes can beextracted by using the keys method.

columns the columns or kinds of things that can be retrieved from the database. As withkeys, all possible columns are returned by using the columns method.

keytype the keytype that matches the keys used. For the select methods, this is used toindicate the kind of ID being used with the keys argument. For the keys methodthis is used to indicate which kind of keys are desired from keys

... other arguments. These include:

pattern: the pattern to match (used by keys)column: the column to search on. This is used by keys and is for when the

thing you want to pattern match is different from the keytype, or when youwant to simply want to get keys that have a value for the thing specified bythe column argument.

fuzzy: TRUE or FALSE value. Use fuzzy matching? (this is used with patternby the keys method)

Value

keys,columns and keytypes each return a character vector or possible values. select returns adata.frame.

18 useDataset

Author(s)

Marc Carlson

Examples

## 1st create a Mart object and specify the datasetmart<-useMart(dataset="hsapiens_gene_ensembl",biomart='ensembl')## you can list the keytypeskeytypes(mart)## you can list the columnscolumns(mart)## And you can extract keys when this is supported for your keytype of interestk = keys(mart, keytype="chromosome_name")head(k)## You can even do some pattern matching on the keysk = keys(mart, keytype="chromosome_name", pattern="LRG")head(k)## Finally you can use select to extract records for things that you are## interested in.affy=c("202763_at","209310_s_at","207500_at")select(mart, keys=affy, columns=c('affy_hg_u133_plus_2','entrezgene'),

keytype='affy_hg_u133_plus_2')

useDataset Select a dataset to use and updates Mart object

Description

This function selects a dataset and updates the Mart object

Usage

useDataset(dataset,mart, verbose = FALSE)

Arguments

dataset Dataset you want to use. List of possible datasets can be retrieved using thefunction listDatasets

mart Mart object created with the useMart function

verbose Give detailed output of what the method is doing, for debugging

Author(s)

Steffen Durinck

Examples

if(interactive()){mart=useMart("ensembl")mart=useDataset("hsapiens_gene_ensembl", mart = mart)}

useEnsembl 19

useEnsembl Connects to the selected BioMart database and dataset hosted by En-sembl

Description

A first step in using the biomaRt package is to select a BioMart database and dataset to use. TheuseEnsembl function enables one to connect to a specified BioMart database and dataset hostedby Ensembl without having to specify the Ensembl URL. To know which BioMart databases areavailable see the listEnsembl function. To know which datasets are available within a BioMartdatabase, first select the BioMart database using useMart and then use the listDatasets function onthe selected BioMart, see listDatasets function.

Usage

useEnsembl(biomart, dataset, host="www.ensembl.org", version = NULL,GRCh = NULL, mirror = NULL, verbose = FALSE)

Arguments

biomart BioMart database name you want to connect to. Possible database names can beretrieved with the function listEnsembl

dataset Dataset you want to use. To see the different datasets available within a biomaRtyou can e.g. do: mart = useEnsembl(’ENSEMBL_MART_ENSEMBL’), fol-lowed by listDatasets(mart).

host Host to connect to if different then www.ensembl.org

version Ensembl version to connect to when wanting to connect to an archived Ensemblversion

GRCh GRCh version to connect to if not the current GRCh38, currently this can onlybe 37

mirror Specify an Ensembl mirror to connect to. The valid options here are ’www’,’uswest’, ’useast’, ’asia’. If no mirror is specified the primary site at www.ensembl.orgwill be used.

verbose Give detailed output of what the method is doing while in use, for debugging

Author(s)

Steffen Durinck

Examples

if(interactive()){

mart <- useEnsembl("ENSEMBL_MART_ENSEMBL")

}

20 useMart

useMart Connects to the selected BioMart database and dataset

Description

A first step in using the biomaRt package is to select a BioMart database and dataset to use. TheuseMart function enables one to connect to a specified BioMart database and dataset within thisdatabase. To know which BioMart databases are available see the listMarts function. To knowwhich datasets are available within a BioMart database, first select the BioMart database usinguseMart and then use the listDatasets function on the selected BioMart, see listDatasets function.

Usage

useMart(biomart, dataset, host="www.ensembl.org",path="/biomart/martservice", port=80, archive=FALSE, ssl.verifypeer =TRUE, ensemblRedirect = NULL, version, verbose = FALSE)

Arguments

biomart BioMart database name you want to connect to. Possible database names can beretrieved with the functio listMarts

dataset Dataset you want to use. To see the different datasets available within a biomaRtyou can e.g. do: mart = useMart(’ensembl’), followed by listDatasets(mart).

host Host to connect to. Defaults to www.ensembl.org

path Path that should be pasted after to host to get access to the web service URL

port port to connect to, will be pasted between host and path

archive Boolean to indicate if you want to access archived versions of BioMart databases.Note that this argument is now deprecated and will be removed in the future.A better alternative is to leave archive = FALSE and to specify the url of thearchived BioMart you want to access. For Ensembl you can view the list ofarchives using listEnsemblArchives

ssl.verifypeer Set SSL peer verification on or off. By default ssl.verifypeer is set to TRUEensemblRedirect

This argument has now been deprecated.

version Use version name instead of biomart name to specify which BioMart you wantto use

verbose Give detailed output of what the method is doing while in use, for debugging

Author(s)

Steffen Durinck

Examples

if(interactive()){

mart = useMart("ensembl")mart=useMart(biomart="ensembl", dataset="hsapiens_gene_ensembl")}

Index

∗Topic methodsattributePages, 2exportFASTA, 3filterOptions, 3filterType, 4getBM, 5getBMlist, 6getGene, 7getLDS, 8getSequence, 9getXML, 10listAttributes, 11listDatasets, 12listEnsembl, 13listEnsemblArchives, 13listFilters, 14listMarts, 15Mart-class, 16NP2009code, 16select-methods, 17useDataset, 18useEnsembl, 19useMart, 20

attributePages, 2

columns (select-methods), 17columns,Mart-method (select-methods), 17

edit, 16exportFASTA, 3

filterOptions, 3filterType, 4

getBM, 5getBMlist, 6getGene, 7getLDS, 8getSequence, 9getXML, 10

keys (select-methods), 17keys,Mart-method (select-methods), 17keytypes (select-methods), 17

keytypes,Mart-method (select-methods),17

listAttributes, 11listDatasets, 12listEnsembl, 13, 19listEnsemblArchives, 13, 15, 20listFilters, 14listMarts, 13, 15, 15

Mart-class, 16

NP2009code, 16

select (select-methods), 17select,Mart-method (select-methods), 17select-methods, 17show,Mart-method (Mart-class), 16

useDataset, 18useEnsembl, 19useMart, 14, 15, 20

21