AMC Cellbiology & Histology AMC Pathology

NKI Sanquin

ACTA VUmc Cellbiology & Immunology VUmc Microbiology VUmc Pathology

NIN

Electron Microscopy in Amsterdam

Part of EMCA= EM Centre Amsterdam

Our moto: Sharing expertise and knowledge by bringing the EM operators together

Electron Microscopy Center Adam

AMC

Wikky Tigchelaar Gutter

Henk van Veen

Daisy Picavet

Anita Grootemaat

Marius van den Bergh Weerman

Per Larson

Nicole van der Wel

EMCA users

NKI

Hans Jansen

Andrea Murachelli

Sasha Fish

VUMC

Jeannette Pankras

NIN

Sharon de Vries

ACTA

Vincent Everts

Sara Sprangers

Yixuan Cao

Gang Wu

Who is part of the

Core Facility Cellular Imaging

Eric Reits

Equipment available:

3 TEMs

FEI Tecnai T12, G2 Spirit Biotwin,+ Veleta and Eagle

FEI Tecnai T12, G2 Spirit Biotwin,+ Veleta

Philips CM-10, + Megaview

1 SEM

Zeiss Sigma Field Emission, Gemini

with SE 1 InLens and Backscatter detector

preparation equipment:

1 Cryo-Ultramicrotome Leica FC6

3 Ultramicrotomes Leica

Plunge freezers

Freeze etching unit BAF 300

Carbon coater Balzers MED 010

Sputter coater SCD040 Balzers

Extra holders:

Multigrid holder

Gatan cryo-holder

Tomography holder and software

What is at the

Techniques available: SEM • Materials/crystals • Biological samples TEM • Immuno-EM • Tomography • Neg staining (vesicles LEKC) • Epon /pathology • Cryo-EM • CLEM

Published in 1 year EMCA:

van der Spek; Endocrinology 2016

Atet et al PLOS Path 2016

200 nm

espG5::tn M.mar wt

Ates et al., PLOS Path 2016

Bedussi et al JCBFM 2016

Published since opening

microbiology

biology

International collaborating institutes

Institute Pasteur Paris France. Harvard Medical School Boston, USA. Albert Einstein College of Medicine, New York USA. Max Planck Institute for Infection Biology, Berlin Germany. Public Health England, Porton Down UK. University of Aberdeen, UK. Universidad de Zaragoza, Zaragoza Spain. National Institutes of Health, Bethesda, USA. National Hansen's Disease Program, USA. Stellenbosch University, South Africa. The Ohio State University. USA. University of Kentucky, USA.

microbiology

biology

Mtb

Mtb

Courses - Graduate School: Basic Course Microscopy (with NKI and VUmc) - OIO&postdoc: Advanced Course Microscopy (with NKI, FNWI, Erasmus and UMCG) - Graduate School: Imaging in medical life sciences (in development) Examples of teaching diploma students: • Bachelor Course: AMC-keuzevak “Moleculaire beeldvorming en nanogeneeskunde” • AUC Amsterdam University College Master Labcourse with ACTA • Biomed Sciences Advanced Microscopy Bachelor • Clin Cell Biology Master course - BWK: bachelor course on cell biology and microscopy - BWK: master tract cell biology & advanced microscopy

The set-up of the

GOAL:

• Make Electron Microscopy available for (Amsterdam) users

• Give guidance and technical support

• Education to PhD, masters students and researchers

Last year

– PhD students were trained:

Andres, Philip, Linda, Gang, Guo, Louis, Maikel, Thijs, Yixuan, Sara

– MSc, BSc students did internship:

Jessie, Enzo, Edwin, Marcella, Zehui, Mandy

The set-up of the

GOAL:

Make Electron Microscopy available for (Amsterdam) users

• Shared costs

• technical support

• ‘Up-to-date’ Medical Electron Microscopy lab

• Biological questions determine the need for new equipment