paul ehrlich and pcsk9 inhibition: a magic bullet for cvd … · 2020-03-10 · e pcsk9 46l...
TRANSCRIPT
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Paul Ehrlich and PCSK9 inhibition: A
Magic Bullet for CVD prevention?
Jacques Genest MD
Cardiovascular Research Laboratories
McGill University Health Center
Endocrinology Rounds 19 Nov 2015
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Merck *
Pfizer
Novartis *
AMGEN *
Cerenis *
Disclosure J. Genest MD 2016
Relevant disclosure: JUPITER, IMPROVE-IT, CANTOS , CAPREE steering Committees; REVEAL , ACCELERATE, AMG145 , Lilly Clinical Trials.
Advisory Board, Speaker’s Bureau, Consultant, Grants, Clinical Trials
Sanofi/Regeneron *
Lilly
Valeant
Aegerion *
Ascati
Stock ownership: none;
Off label use: none
* Scientific Advisory
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Monoclonal Antibodies (mAbs) 101
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Paul Ehrlich (1854-1915)
• Gram stain
• Arsphenamine (first
Rx for Syphilis)
• Anti-serum against
dyphteria
• Concept of
“magische Kugel” –
magic bullet
Nobel Prize 1908
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Polyclonal Abs:
• Recognize multiple
epitopes on same
antigen
• Usually high affinity
BUT
• Show major batch to
batch variability
• Can include relatively
non-specific antibodies
Polyclonal Antibodies
B cell (humoral) immune responses are polyclonal
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Monoclonal Antibodies (MAbs)
Monoclonal Abs:
• High specificity; detect
only one epitope on
antigen
• High homogeneity;
once made, antibodies
are constant, all
batches identical
• Can be produced in
unlimited quantities
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Alirocumab Manufacturing –
Production / Purification
Thaw 3 days 7 days 6-8 days
Production:
13-14 days
Vial of Cells Shaker Flask WAVE Bioreactor
Seed Train
Bioreactors:
50-3000L
Production
Bioreactor:
10,000L
Through a series of centrifugation, filtration membranes, and chromatographic steps, the antibody is purified to the desired quality for human use.
10,000L Bioreactor
14 Days
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Antibody technology has evolved over past decades
Red = mouse
Blue = human
Imm
un
og
en
icit
y
Fully Mouse
1st generation
Highly Immunogenic
Chimeric
2nd generation
e.g.Abciximab
Chimeric,
Still very immunogenic
Humanized
3rd generation
e.g.Bococizumab
Can be time-consuming to create
“Fully” Human
4th generation
e.g.Evolocumab and Alirocumab
repeated dosing possible
8Nomenclature: Prefix (Pharma) C (Cardiovascular) UMAB
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Monoclonal antibodies in the clinic
Over 30 monoclonal antibodiesapproved for clinical use byEuropean/US regulatory agencies in,for example:
• Asthma• Autoimmune diseases• Oncology• Ophthalmic disorders
Approximately 235 monoclonalantibodies in active trials, for example:
• Alzheimer’s disease• Cardiovascular disease• Infectious disease• Osteoporosis
Cumulative number of human monoclonal antibodies entering clinical study between 1985
and 2008
100
0
Nu
mb
er o
f cl
inic
al c
and
idat
es
Year
90
80
70
60
50
40
30
20
10
110
120
130
140
150 All human monoclonal antibodies
Antineoplastic only
Immunomodulatory only
Anti-infectious only
Other indications
1. Adapted from: Nelson AL et al. Nat Rev Drug Discov 2010;9:325–38; 2. Landes Bioscience (2014). mAbs: About this journal. Available at: http://www.landesbioscience.com/journals/mabs/about/. Accessed July 2014; 3. ClinicalTrials.gov (July 2014). Available at: http://www.clinicaltrials.gov/.
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The biologics explosion
TNF Inhibitors
Etanercept
Adalimumab
Infliximab
Golimumab
Certolizumab
Non-TNF Biologics
• Tocilizumab (IL-6)
• Rituximab (B cell CD20)
• Abatacept (B7-CTLA4Ig)
• Anakinra (IL1)
• Canakinumab IL-1b)
Emerging non-TNF Biologics
•Sarilimab
•Olokizumab
•Clazakizumab
•Sirukumab
•Secukinumab
•Brodalumab
(IL6R)
(IL6)
(IL6)
(IL6)
(IL-17)
(IL-17R)
Emerging non-MAb Biologics
•Small molecules
• Tofacitinib (JAK1/3)
•Biosimilars
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PCSK9
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PCSK9: A Canadian Discovery
Dr. Nabil Seidah IRCM
Nature Genetics 34, 154 - 156 (2003)
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Proprotein Convertase
Subtilisin/Kexin Type 9
Evolutionary Conservation: Must be important
Bacillus amyloliquefaciens saccharomyces cerevisiae Homo sapiens
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ER TGN
EndosomeLysosome
LDL-R
PCSK9
pre-PCSK9
A:LDL-R pathway in
absence of PCSK9
B:Intracellular
PCSK9 route
C:Extracellular
PCSK9 route
Mature
PCSK9
LDL
apoB
PCSK9
Deg
rad
ati
on
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PCSK9 as a Target
Cohen JC, et al. NEJM 2006;354:1264
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Patients with Genetically Lower LDL have
Correspondingly Better CV Event Reduction
10%
20%
30%
.003 .005 .078 .104 .130 .155 .181 .207 .233 .259 .285 .311 .330 .363 .389 .389 .440 .466 .492 .518 .5440
Lower LDL-C (mmol/L)
Pro
po
rtio
nal
Ris
k R
edu
ctio
n (
SE)
log
scal
e
PCSK9 46Lrs11591147
ALLHAT-LLT
SEARCH
Pharmacologically Lower LDL-C
A to Z
Genetically Lower LDL-C
GISSI-P
NPC1L1 LDL-C ScoreHMGCR LDL-C Score
IMPROVE-IT
Combined NPC1L1 & HMGCR LDL-C Score
LDLRrs6511720
LDLRrs2228671
Ference BA, et al. J Am Coll Cardiol. 2015;65(15):1552-1561.
PCSK9rs11206510
ABCG5/8rs4299376
HMGCRrs12916
PCSK9rs2479409
NPC1L1rs217386
HMGCR LDL-C Score
NPC1L1 LDL-C Score
Greater effect than Pharmacologically Lower LDL-C
− Possibly due to Lifetime Lower LDL levels
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LDLR
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Clinical Data
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ODYSSEY Long-Term: Alirocumab Plus Statin
Achieved a 62% Reduction in LDL-C over
Placebo+Statin at 24 weeks
Robinson J, et al. N Engl J Med. 2015;372(16):1489-99.
3.60
3.00
2.40
1.80
1.20
0.60
0.00
Me
dia
n L
DL
-C (
mm
ol/
L)
Week
0 4 8 12 16 24 36 52 64 78
0.8%
3.08 mmol/L 3.17 mmol/L
3.6%
1.25 mmol/L
-61.0%
1.50 mmol/L
-52.4%
Alirocumab + statin therapy at maximum tolerated dose ± other LLT (150 mg q2w)Placebo + statin therapy at maximum tolerated dose ± other LLT
Placebo
Alirocumab
780
1530
754
1473
747
1458
746
1436
716
1412
708
1386
694
1359
676
1349
659
1324
652
1269
No. of patients with data available
62% reduction, P<0.001
Absolute reduction: 1.2 mmol/L
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ODYSSEY Long-Term: Reduction in the Rate of
Cardiovascular Events- Post-hoc Analysis
*Primary endpoint for the ODYSSEY OUTCOMES trial: CHD death, Non-fatal MI, Fatal and non-fatal ischemic stroke, Unstable angina requiring hospitalization. LLT, lipid-lowering therapy† ≥52 weeks for all patients continuing treatment, incl. 607 patients who completed W78 visit
Robinson J, et al. N Engl J Med. 2015;372(16):1489-99.
788
1550
776
1534
731
1446
703
1393
682
1352
667
1335
321
642
127
252
847260483624120WeeksNo. at Risk
Placebo
Alirocumab
Kaplan-Meier Estimates for Time to First Adjudicated Major CV Event
Cu
mu
lati
ve p
rob
abili
ty o
f ev
ent
0.06
0.05
0.04
0.03
0.02
0.01
0.00
Alirocumab + max-tolerated statin ± other LLT (150 mg q2w)Placebo + max-tolerated statin ± other LLT
54% RRR
Safety Analysis†
Cox model analysis
HR 0.46
95% CI: 0.26 to 0.82
P<0.01
Kaplan-Meier Estimates for Time to First Adjudicated Major CV Event*
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ODYSSEYLong-Term
Ratio of LDL Lowering to CV Event Reduction with
PCSK9 Inhibitors Holds True to the
“LDL Hypothesis”
Waters DD. Hsue PY. Circ Res. 2015;16:1643-1645.
70%
60%
50%
40%
30%
20%
10%
0%
-10%
0.5 1.0 1.5 2.0
Pro
po
rtio
nal
red
uct
ion
in e
ven
t ra
te (
SE)
Reduction in LDL cholesterol (mmol/L)
OSLER
IMPROVE-IT
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Am J Cardiol 2015;115:1212e1221)
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Christie M. Ballantyne, et al.
Results of Bococizumab, A Monoclonal Antibody Against Proprotein Convertase Subtilisin/Kexin Type 9, from a
Randomized, Placebo-Controlled, Dose-Ranging Study in Statin-Treated Subjects With Hypercholesterolemia
The American Journal of Cardiology, Volume 115, Issue 9, 2015, 1212–1221
Bococizumab
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Figure 4. Mean percentage change from baseline in LDL-C. Change over time is
shown for the (A) Q14 days and (B) Q28 days placebo and bococizumab dose
groups.
Ballantyne CM. The American Journal of Cardiology, 2015;115:1212–1221
Bococizumab
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Bococizumab: AE
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FDA approves Praluent to treat certain patients with high cholesterol
PCSK9 mAb
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Praluent is approved for use in
addition to diet and maximally
tolerated statin therapy in adult
patients with heterozygous
familial hypercholesterolemia
(HeFH) or patients with clinical
atherosclerotic cardiovascular
disease such as heart attacks or
strokes, who require additional
lowering of LDL cholesterol.
PCSK9 mAb (US)
Repatha is approved for use in
addition to diet and maximally-
tolerated statin therapy in adult
patients with heterozygous
familial hypercholesterolemia
(HeFH), homozygous familial
hypercholesterolemia (HoFH), or
clinical atherosclerotic
cardiovascular disease, such as
heart attacks or strokes, who
require additional lowering of
LDL cholesterol.
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Anti-drug Antibodies (ADA)
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Anti-drug antibodies (ADA):
the challenge
Immunogenicity:
The potential for an antigen to
induce an immune response
Immunogenicity against
therapeutic proteins that are
not in the normal human
repertoire is a normal immune
response.
Reaction to neo-antigens
Proteins are non-human
Fusion proteins create new
epitopes
Unusual glycosylation
Anti-drug antibody
formation
Anti-PSCK9
Antibody Fc
Antibody Fab
(Neutralizing)
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Anti-PCSK9 Antibodies
FDA Briefing Document Praluent (alirocumab) injection June 9, 2015;
FDA Briefing Document Evolocumab June 10, 2015.
Evolocumab
• Overall incidence of anti-evolocumab binding antibodies after at least one dose of evolocumab was 0.3% (13 of 4915)
• Responses were of low-titer, most were transient
• No neutralizing antibodies have been detected
• No impact of binding antibodies on safety, pharmacokinetics, or pharmacodynamics
Alirocumab
• Observed in 4.8% of patients following alirocumab treatment vs. 0.6% of patients in control group
• Most responses were of low-titer, non-neutralizing, and/or transient
• Neutralizing antibodies were reported in 1.2% of patients treated with alirocumab
The detection of anti-drug binding antibody formation is highly dependent on the
sensitivity and specificity of the assay. Comparison of the incidence of antibodies to
evolocumab with incidence of antibodies to other products may be misleading.
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Lancet 383;9911, 60–68
PCSK9 RNAi
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PCSK9 mAb Clinical Indications
Familial Hypercholesterolemia
ASCVD (Atherosclerotic Cardiovascular
Disease) not at goal despite maximally tolerated
lipid-lowering therapy
Statin intolerant
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FH + CAD
PCSK9 mAb: Whom, When?
FH Not @ Goal
CAD* Not @ Goal
Hi Risk Not @ Goal
Numbers (Guess)
10,000
10,000
250,000
CAD* Approx 1.5 M CDN
>250,000
20-30 HoFH
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PCSK9 and Diabetes
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39
Background: Statin Treatment and
Increased Risk of T2DM
CI, confidence interval; OR, odds ratio; T2DM, type 2 diabetes mellitus.
Case = developed T2DM. Non-case = did not develop T2DM.Swerdlow DI, et al. Lancet. 2015;385:351–361.
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Henry N Ginsberg1, Michel Farnier2, Jennifer G Robinson3, Christopher P Cannon4, Naveed Sattar5, Marie T Baccara-Dinet6, Christelle Lorenzato7, Maja Bujas-Bobanovic8, Michael J Louie9,
Helen M Colhoun10
1Columbia University, New York, NY; 2Point Medical, Dijon, France; 3University of Iowa, Iowa City, IA; 4Harvard Clinical Res Inst, Boston,
MA; 5University of Glasgow, Glasgow, UK; 6Sanofi, Montpellier, France; 7Sanofi, Chilly-Mazarin, France; 8Sanofi, Paris, France;
9Regeneron Pharmaceuticals, Tarrytown, NY; 10University of Dundee, Dundee, UK.
Efficacy and Safety of Alirocumab: Pooled
Analyses of 1051 Individuals
with Diabetes Mellitus from
Five Placebo-Controlled Phase 3 Studies of at
least 52 weeks’ duration
This study was funded by Sanofi and Regeneron Pharmaceuticals, Inc.
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Mean Fasting Plasma Glucose Over Time
(Safety Population)
41
†75 mg Q2W increased to 150 mg Q2W at W12 if LDL-C levels at Week 8 were ≥70 mg/dL (1.81 mmol/L).
FPG, fasting plasma glucose.
5
6
7
8
9
10Alirocumab 75/150 mg Q2W †
5
6
7
8
9
10Alirocumab 150 mg Q2W
Placebo with DM
Alirocumab with DM
Placebo without DM
Alirocumab without DM
Me
an
(S
E)
FP
G, m
mo
l/L
12 24 36 520 241236 52 0
Week Week
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Mean HbA1c Over Time (Safety Population)
42
†75 mg Q2W increased to 150 mg Q2W at Week 12 if LDL-C levels at Week 8 were ≥70 mg/dL (1.81 mmol/L).HbA1c, glycated hemoglobin.
Me
an
(S
E)
Hb
A1
c,
%
Placebo with DM
Alirocumab with DM
Placebo without DM
Alirocumab without DM
5
5.5
6
6.5
7
7.5
8
8.5
9
9.5
10 Alirocumab 75/150 mg Q2W †
5
5.5
6
6.5
7
7.5
8
8.5
9
9.5
10 Alirocumab 150 mg Q2W
12 24 520 0 12 24 52
Week Week
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Evaluation of the One-Year Efficacy, Safety and Glycaemic Effects of Evolocumab
(AMG 145) in 4,802 Subjects With, at High Risk for, or at Low Risk for, Diabetes Mellitus
Naveed Sattar,1 David Preiss,1 Dirk Blom,2 C. Stephen Djedjos,3 Mary Elliott,4 Andrea Pellacani,3 Scott M Wasserman,3 Michael Koren,5 Rury Holman6
1BHF Cardiovascular Research Centre, University of Glasgow, UK; 2Division of Lipidology, Department of Medicine, University of Cape Town, South Africa; 3Amgen Inc., Thousand Oaks, CA, USA; 4Amgen Limited, Cambridge, UK; 5Jacksonville Center for Clinical Research, Jacksonville,
FL, USA; 6Diabetes Trials Unit, OCDEM, University of Oxford, UK
European Association for the Study of DiabetesStockholm, Sweden
17 September, 2015 – Session OP 27
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Results: Median Fasting Plasma Glucose
Over One Year*
*48 weeks of open-label treatmentError bars represent SE of the medianSoC, standard of care; T2DM, type 2 diabetes mellitus
44
Med
ian
Glu
co
se, m
mo
l/L
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Results: Median HbA1c Over One Year*
*48 weeks of open-label treatmentError bars represent SE of the medianHbA1c, glycated haemoglobin; SoC, standard of care; T2DM, type 2 diabetes mellitus
45
Me
dia
n H
bA
1c,
%
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Conclusion
• In patients with T2DM, or people at high or low
risk of T2DM, one year of treatment with
evolocumab or alirocumab:
– markedly reduced LDL-C in all groups
– showed encouraging safety
– showed no measurable effect on glycaemic
parameters including new-onset T2DM vs SoC
alone
• Clinical trials are ongoing to examine the effects
of evolocumab on patients with T2DM, and on the
incidence of new-onset T2DM
46