a n m - .urn Centrifuge Model Nos. 4207, 4208, 4209

Printed in U.S.A.

Clay Adams Dlvlsion of Becton Dicklnson and Company

In Canada: Becton Dlcklnson and Company, Canada Ltd.,

@#

4215-000-000 REV. B ( 1 /85)

OPERATOR'S MANUAL

Clay Adams

(Model Nos. 4207, 4208, 4209)

OPERATOR'S MANUAL

QBC, VACUTAINER, Clay Adams, and are trademarks of Becton Dickinson and Company..

Copyright @ 1983 by CLAY ADAMS, Division of Becton Dickinson and Company.

CONTENTS

SECTION 6 . TEST PROCEDURES SECTION 1 -INTRODUCTION

1.1 Intended Use ................................................ 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 Summary and Explanation I .... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.3 Principles of the Procedure 1 .

SECTION 2 . PRINCIPLES OF OPERATION .................................................. 2.1 Description 2

................................................ 2.2 Specifications 3

SECTION 3 . INSTALLATION REQUIREMENTS

............................. 3.1 Rotor Head Assembly Procedures 4 ........................................ 3.2 Centrifuge Placement 5

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.3 Electrical Requirements 5

SECTION 4 . OPERATING INSTRUCTIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.1 Loading and Balancing Rotor Head 6

................................. 4.2 Securing Head Cover and Lid 6 ....................................... 4.3 Starting the Centrifuge 7

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.4 Stopping and Cover Removal 7 ............................. 4.5 Handling Centrifuged QBC Tubes 8

.................................. 4.6 Periodic Calibration Checks 8

4.6.1 Centrifugespeed ....................................... 8 ......................................... 4.6.2 Timer Accuracy 8

........................... 4.7 Operating Precautions and Hazards 9

4.7.1 BasicProcedures ....................................... 9 4.7.2 PeriodiclnspectionofRotorHead ....................... 9

..................................... 4.8 Maintenance and Service 10

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.8.1 General Cleaning 10 4.8.2 Rotor Inspection and Cleaning Procedure ................ 10

............................................. 4.8.3 Lubrication 11 ................. 4.8.4 Troubleshooting and Parts Replacement 11

SECTION 5- SPECIMEN COLLECTION. PREPARATION AND STABILITY

............................................ 5.1 QBC Blood Tubes 12 5.2 BloodCol!ection andTubeFilling ............................. 12

........................................... 6.1 Materials Provided 13 ......................... 6.2 Materials Required. But Not Provided 13

..................................... 6.3 Centrifugation Procedure 13

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.3.1 Temperature Requirements 13 . . . . . . . . . . . . . . . . . . . . . . . . 6.3.2 Loading and Balancing the Head I3

............................... 6.3.3 Centrifugation Procedure 13

SECTION 7. TEST RESULTS AND PERFORMANCE

7.1 Test Results ................................................. 15 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.2 Limitations of the QBC Procedure 15

............................................. 7.3 Expected Values 15 .......................... 7.4 Specific Performance Characteristics 15

. . . . . . . . . . . . . APPENDIX A -- PARTS REPLACEMENT PROCEDURES A-1

........................ . APPENDIX B ILLUSTRATED PARTS LIST B-1

................................. APPENDIX C -- WIRING DIAGRAM C-1

iii

Section 1 lNTRODUCTlON

1.1 INTENDED USE The QBC Centrifuge is a high speed laboratory instrument designed to produce packed cell volumes for the quantitative measurement of hemato- logic values by the QBC method.

1.2 SUMMARY AND EXPLANATION

The Centrifuge is specifically designed as a tube-preparation accessory to B the Clay Adams Q8C Series Centrifugal Hematology Systems. Special-

purpose QBC blood tubes can be accommodated which, under centrifuga- tion, differentiate and expand the cell layers of the buffycoat. When measured on a QBC Reader, the tubes yield a hematocrit, as well as the platelet count, total white cell count and counts of the grouped white-cell subpopulations, granulocytes and lymphocytes/monocytes.

1.3 PRINCIPLES OF THE PROCEDURE

The QBC Series Centrifugal Hematology Systems utilize cell staining and density-gradient centrifugation to permit the determination of white cell and platelet counts (in addition to hematocrit) by means of a modified microhematocrit procedure. In the QBC method, cells settling in the buffy coat (ieukocytes and platelets) are stained by a coating reagent within the blood tube, and the resulting packed cell layers are mechanically expanded during high-speed centrifugation by means of a precision float. Lengths of the differentiated cell layers (Figure 1) in the centrifuged tube are then measured in a special electro-optical QBC Reader. Hematocrit, Platelet Count, White Cell Count, and counts of the Granulocyte and Lymphocyte/ Monocyte subpopulations are automatically computed from the length measurements."

WHITE CELLS WHiTE CELLS ( GRANULOCYTES ] 7 r- ( LYMPHOCYTES & MONOCYTES )

RED CELLS--\ \ / - PLATELET LAYER - --- RED CELLS- FLOAT \-PLASMA AROUND FLOAT

Figure 1. Differentiated Cell iayers in Centrifuged QBC Blood Tube.

'See applicable QBC System Operator's Manual for details on test methodology.

1

Section 2 . PRINCIPLES OF OPERATION

2.1 DESCRIPTION

The QBC Centrifuge is a compact, high speed machine incorporating a 12-place rotor and fixed 5-minute timing cycte. Main components and operator controls are shown in Figure 2.

\;% ,/ ,Rotor Retaining \% / Nut

Safety Switch

Figure 2. Assembly Details, QBC Centrifuge.

The rotor head is a single-piece aluminum assembly with equally spaced slots to accommodate up to 12 QBC Blood Tubes. The protective metal head cover, which ~solates the blood tubes during centrifugation, incorporates a threaded hub for ease of installation and removal. A safety switch located within the upper front housing prevents the motor from operating until the clear plastic lid is closed and latched.

Centrifugation time is fixed at 5 minutes by an internal electronic timer. The PUSH-TO-START/PUSH-TO-STOP button controls power to the motor and operates in conjunction with the lid safety switch and timer. The button is illuminated when the motor is powered. Upon completion of the 5-minute spin cycle, the button is automatically re-set to the stop (off) position and must be pressed again to start the next centrifugation. Once started, the Centrifuge can be stopped any time before completion of the cycle by pressing the button.

A sturdy single-piece housing made of die-cast metal provides a stable mounting structure for the centrifuge motor. The motor IS assembled onto three ball-type shock absorbers to cushion vibrations and minimize noise. The complete Centrifuge rests on four rubber feet.

@ Speed @ rated voltage: approx. 12,000 RPM

@ Relative Centrifugal Force (@ rated voltage ): approx. 14,387 x g.

Timer: Electronic, with fixed 5-minute cycle.

@ Safety Interlock Switch - actuated by the lid latch. The Centrifuge motor is inoperable unless the safety interlock switch is actuated (i.e., lid fully closed and latched).

Electrical:

Model No. VoltslFrequencylAmps Voltage Tolerance

4207 1 20V/60Hz/4 amps 105-130 volts 4208 100V/50 or 60Hz/4 amps 90-1 10 vol ts 4209 220V/50Hz/2 amps 198-242 VOI ts

@ Motor Brake - electric-type, automatically actuated by timer after com- pletion of spin cycte or by pressing PUSH-TO-STOP Button.

Power Cord - UL Listed 2.0 m (6.5 ft), strain relieved power cord with 3-prong grounded plug.

e Weight:

Centrifuge with assembled Rotor Head and Head Cover: 5 kg (1 1 Ibs)

Shipping Weight: 7.5 kg (16.5 Ibs).

@ Dimensions:

Closed Machine: 21.6 cm (8.5 in.) high x 25.4 cm (10.0 in.) deep x 21.6 crn (8.5 in.) wide.

Shipping Container: 30.5 cm (12 in.) x 30.5 crn (12 in.) x 30.5 crn (12 in.)

Section 3 INSTALLATION REQUIREMENTS

3.1 ROTOR HEAD ASSEMBLY PROCEDURES

The unassembled rotor head and head cover are separately boxed inside the -

Centrifuge shipping container. Included hardware are a flanged head nut and wrench for attaching the rotor head to the motor shaft.

Figure 3. Head Assembly Details.

To install the rotor head, refer to Figure 3 and perform the following:

a. Open the Centrifuge lid. Remove the shipping tape from the Motor Shaft Adapter.

b. Place the "DM-shaped hole of the Rotor onto the threaded "D"-shaped rotor shaft. Be sure the Rotor is fully bottomed on the Shaft and Shaft Adapter.

c. Thread the Head Nut onto the Shaft, flanged side up as illustrated.

d. Place the Wrench pins into the holes of the Nut and, holding the Rotor Head with one hand, turn the Wrench clockwise to tighten.

3.2 CENTRIFUGE PLACEMENT

Always place the QBC Centrifuge on a stable, level table or counter surface.

Be sure to locate the Centrifuge where it will NOT BE EXPOSED TO DIRECT SUNLIGHT. Centrifugations in direct sunlight can elevate the rotor tempera- ture and may adversely affect cell banding in QBC Blood Tubes. See Section 6 for special temperature requirements of QBC tests.

% 3.3 ELECTRICAL REQUIREMENTS

The QBC Centrifuge is available in three models, each designed for the different electrical service listed below.

"P Centrifuge Electrical Rating Model No. Vol tage/Frequency

Al l models incorporate a 3-prong plug on the power cord for connection to a grounded efectrical outlet.

~ CAUTION

Always connect the power cord to a &wire grounded electrical outlet rated at the same voltage and frequency specified on the data plate on the bottom of the Centrifuge. Where only a 2-wire receptacle is available, have it replaced with a properly grounded 3-wire receptacle by a qualified serviceman and in accordance with the National Electrical Code. Do not remove the grounding prong from the power cord. If an extension cord is required, use only a 3-wire grounded cord having the propervoltage and current rating.

Section 4 OPERATING INSTRUCTlONS

This section contains general procedures for use and operation of the QBC Centrifuge. Additional tube handling and operating instructions will be found in Section 6 - "TEST PROCEDURES".

4.1 LOADING AND BALANCING ROTOR HEAD

Tube positions on the rotor head are numbered 1-12 for specimen identification.

Always place blood tubes on the head in a balanced array. When spinning an odd number of tubes, balance the head by placing an empty QBC blood tube, fitted with a Closure and Float, into the carrier-slot position opposite the odd tube. When balancing tubes, be sure to maintain proper identifica- tion of test specimens.

To place a blood tube on the rotor head, insert the open end of the tube under the flange of the head nut, then lower the tube into the tube slots (Figure 4). Position the closure snugly against the outer rim of the head.

IMPORTANT: Before installing the head cover, be sure the closure of each QBC blood tube rests against the outer rim of the head.

Figure 4. Placing QBC Tubes Onto Rotor Head.

4.2 SECURING HEAD COVER AND LID

CAUTION

TO PREVENT TUBE BREAKAGE AND LOSS OF SAMPLES, NEVER CENTRIFUGE BLOOD TUBES UNLESS THE METAL HEAD COVER IS INSTALLED.

L

To install the Head Cover: place the Cover onto the threads of the motor shaft and turn the knurled headcover nut clockwise. Hold the head cover with one

hand and continue turning the head nut clockwise until tight. Do NOT tighten with the head nut Wrench.

Figure 5. Installing Head Cover. Figure 6. Securing Lid Latch.

Zfose the Centrifuge lid and press firmly down until the latch handle of the lid snaps into the latch block on the housing (Figure 6).

NOTE: The internal safety switch will not be actuated and the Centrifuge will not operate unless the lid is securely latched.

4.3 STARTING THE CENTRl FUGE

To start the Centrifuge, lightly press the white "PUSH-TO-START/PUSH-TO- STOP" button: The button is illuminated when the motor is powered. Rated speed is reached within approximately 15 seconds.

4.4 STOPPING AND COVER REMOVAL

When the 5-minute centrifugation period has elapsed, the button light will turn off and an electrically-actuated brake wilt cause the head to decelerate. Head rotation will stop within approximately 30 to 35 seconds after the button light turns off.

IMPORTANT: To stop the Centrifuge any time before completion of the timed cycle, press the "PUSH-TO-START/PUSH-TO-STOP" button once. NOTE: If the Centrifuge is stopped any time before the end of the complete cycle, the Timer automatically re-cycles to zero.

i

CAUTION

TO PREVENT HAZARDS, NEVER ATTEMPT TO OPEN THE LID UNTIL THE HEAD STOPS ROTATING.

To remove the Head Cover: hold the cover with one hand while turning the cover nut counterclockwise. Note: if the cover nut is too tight to loosen by hand, loosen with the Wrench.

1 I I 4.5 REMOVING AND HANDLING CENTRIFUGED QBC TUBES

Always remove QBC blood tubes from the Rotor PROMPTLY AFTER THE CENTRIFUGATION CYCLE IS COMPLETED.

Do NOT pick up or handle QBC tubes below the plasma column, since the packed cell bands can be disturbed by heat transferred from the fingers.

Consult TEST PROCEDURES in Section 6 for details on the correct handling and storage of centrifuged QSC tests.

I

CAUTION

TEST VALUES FROM QBC BLOOD TUBES CAN ONLY BE OBTAINED ON A QBC SERIES TUBE READER INSTRUMENT. DO NOT ATTEMPT TO READ QBC TUBES ON HEMATOCRIT SCALE PLATES OR WITH MECHANICAL TUBE READING DEVICES. 1

4.6 PERlODlC CALIBRATION CHECKS

4.6.1 Centrifuge Speed

The QBC Centrifuge is a fixed-speed machine, designed to operate within a speed range of 10,500 to 13,100 RPM at all voltages. Speed should be checked periodically with a tachometer to determine that the RPM is within permissible tolerances. Perform the check with the head and head cover instailed and the lid closed. Use of an ADAMS Photo Electric Tachometer (Catalog No. 5205) or equivalent device is recommended.

In most cases, speed will vary, depending on motor characteristics and fluctuations in line service voltage above or below the rated voltage. Centrifuges must not be operated outside the following voltage ranges:

Model 4207: 105 volts to 130 volts.

Model 4208: 90 volts to 1 f 0 volts.

Model 4209: 198 volts to 242 volts.

Over these voltage ranges, speed should be between 10,500 RPM to 13,100 RPM. (NOTE: RPM's below 10,500 may indicate that the governor brushes are worn and require factory replacement.) If the speed check is outside tolerances, contact your Clay Adams dealer for service.

4.6.2 Timer Accuracy

Periodically, the 5-minute internal timer should be checked for accuracy against a stopwatch as follows:

a. With the head and head cover installed, close and latch the lid.

b. Press the "PUSH-TO-START" button and simultaneously start the stopwatch.

c. Check the length of time for the light in the button to turn off against the stopwatch. The button light should turn off within 5 minutes .rt 30 seconds.

d. If the button light does not turn off within 5 minutes f 30 seconds, contact your Clay Adams dealer for service.

4.7 OPERATING PRECAUTIONS AND HAZARDS

4.7.1 Basic Procedures

In order to obtain properly centrifuged specimens and to avoid damage or hazards, the foilowing basic operating precautions should be carefully observed:

@ Operate the Centrifuge only at the line voltage and frequency specified on the data plate and from a grounded electrical outlet only.

For smooth operation and long service life, always place blood tubes in the head in a balanced array.

e Always install the head cover before operating the centrifuge.

Unplug the power cord before attempting to service the centrifuge.

@ Never attempt to override the lid safety switch of the centrifuge.

Observe good laboratory procedures when handling blood specimens.

4.7.2 Periodic Inspection of Rotor Head

The aluminum rotor of the QBC Centrifuge must be inspected as described below for cracks or defects that could impair its continued safe use.

Remove and inspect the rotor for defects at least every 4 months and each time it is disassembled for cleaning. Carefully inspect all top and bottom surfaces, particularly around the center region of the rotor. Install a new replacement rotor assembly if any of the following defects are observed:

e Cracks, especially hairline cracks in the region adjacent to the motor shaft;

s Corrosion on surfaces especially in the region of the motor shaft;

Deep Scratches;

@ Severe Dents.

While the rotor is off the centrifuge, also inspect the motor shaft, shaft adapter and head nut for signs of wear, corrosion or damage. Replace any defective parts. Do not use the centrifuge until i t is repaired.

Safe, trouble-free service also requires that the rotor assembly be cleaned only with the approved agents and by the cleaning procedures specified in 4.8.2 below.

4.8 MAINTENANCE AND SERVICE b. Rinsing and Drylng Procedures

4.8.1 General Cleaning

Use soap or a mild detergent and water to clean the lid, cover, and other parts of the Centrifuge. (See 4.8.2 for special Rotor Cleaning instruc- tions.) To avoid scratching or damaging, do not use solvents or strong abrasives. Dry with soft tissue or cloth.

4.8.2 Rotor Inspection and Cleaning Procedure

INSPECT THE ROTOR FOR DEFECTS EVERY 4 MONTHS AS DESCFllBED IN SECTION 4.7.2

Cleaning and Disinfecting the Rotor:

CAUTION

TO CLEAN THE ROTOR ALWAYS DISASSEMBLE AND REMOVE THE ROTOR FROM THE CENTRI'FUGE. Approved cleaning procedures require immersion, rinsing and drying.

a. Approved Cleaning and Disinfecting Agents

The following agents are approved and recommended for cleaning and disinfecting the rotor:

Actionox (Scientific Products) Liqui-NOX (Atconox, Inc.) Haemo-Sol (Meinecke & Co.) 70% Ethanol or fsopropanol

DO NOT USE any of the following corrosive agents:

* CLOROX* (Sodium Hypochlorite Solution - see Note below) Acid Dichromate Cleaning Solutions

* Hydrogen Peroxide @ Sodium Hydroxide * Hematology Lysing Agent * Sodium Carbonate

Saline Solutions o Alconox

Hydrochloric Acid * Acetic Acid

NOTE: While CLOROX or other mild sotutions of sodium hypochforite are not recommended for cleaning, they may be used where necessary, provided the rotor is thoroughly rinsed and dried as described in Paragraph b.

'Trademark of Clorox Company,

After cleaning or disinfecting with an approved agent, completely immerse and thoroughly wash off the rotor in clean water. Then rinse again under running water to remove all traces of the cleaning agent*

Thoroughly dry the top and bottom surfaces of the rotor before re- installing. Oven-drying may be used, provided oven temperature DOES NOT EXCEED 125" F (52" C).

IMPORTANT

The motor shaft and attaching hardware must be clean and dry before re-assembly.

If it is necessary to clean the rotor without removing it from the cen- trifuge (not a recommended procedure), wipe only with a cloth moistened with water. Avoid wetting the hub area. If accidentally wetted, remove the rotor and dry aff parts. Do NOT wipe with any cleaning agent, since it cannot be sufficiently rinsed off while the rotor is installed.

4.8.3 Lubrication

The Centrifuge motor incorporates permanently lubricated bearings. Lubrication should not be required for the life of the equipment.

4.8.4 Troubleshooting and Parts Replacement

Failure of the Centrifuge motor to start may be due to one of the follow- ing operator-correctable conditions:

Blown line fuse on the timer circuit board; or Worn motor brushes.

The lamp in the PUSH-TO-START/PUSH-TO-STOP Button provides a quick indication of whether the line fuse has blown. If the lamp turns ON, the fuse is not blown and the motor brushes are most Iikely at fault. Both conditions can be remedied by performing the parts replacement pro- cedures in Paragraphs 1 and 2 of Ap~endix A.

If the Centrifuge still will not operate after the checks and replacements described in Appendix A, request Technical Assistance.

In addition to the fuse and motor brushes, other operator replaceable parts are the Lid Seal Gasket (see procedures in Paragraph 3 of Appen- dix A) and the Rotor Head. To replace the Rotor Head, follow the installa-

.t ion instructions in Section 3.1.

Appendix B contains a complete list of replacement parts for the QBC Centrifuge. A wiring diagram of the centrifugeis included in Appendix C.

Section.5 SPECIMEN COLLECTION, PREPARATION AND STABILITY

Section 6 TEST PROCEDURES

CAUTION

WHOLE BLOOD SPECIMENS MAY CONTAIN THE HEPATITIS CAUSATIVE AGENT. USE APPROVED LABORATORY TECH- NIQUES WHEN COLLECTING AND HANDLING PATIENT SPECIMENS.

6.1 MATERIALS PROVIDED

The QBC Centrifuge is provided with all components required for the cen- trifugation of QBC Blood Tubes.

d

6.2 MATERIALS REQUtRED, BUT NOT PROVIDED

The following disposables and test accessories are required for QBC centri- fugation procedures:

5.1 QBC BLOOD TUBES

Blood for testing by the QBC method can be of venous or capillary origin. Two different types of QBC tube devices are utilized, depending upon origin of the blood sample (figures 7 and 8). Both blood tubes are 75 mm in length and of uniform-bore glass.

CLOSURE

RED PLASMA LEVEL

LINES FLOAT

I 1 1 - - (FILL END) BLACK

FILL LINE

Figure 7. QBC Tube Device for Venous Blood.

BLACK FILL LINES -.

FLOAT 55-66 p1 CLOSURE - L: I I (FILL END)

Figure 8. QBC Tube Device for Capillary Blood.

5.2 BLOOD COLLECTION AND TUBE FILLING

QBC Tubes for Venous Blood are filled by means of a semi-automatic pipetter supplied with the QBC System; collection into Capillary Tubes is accom- plished directly from the finger puncture site by capillary action. After filling, ' the tubes are sealed with the plastic closure and the float is inserted. The float expands the buffy-coat cell layers during centrifugation, as previously descri bed.

For detailed instructions on blood coltection, tube preparation and stabi tity of blood for QBC tests, refer to the QBC System Operator's Manual or the Package Insert supplied with each type of QBC blood tube.

QBC Venous- or Capillary-Blood Tubes; QBC Series Tube Reader; QBC Tube l ncu bator (where applicable); QBC Pipetter (for Venous-Blood Only); QBC Work Station;

* Lint-free Wipers.

6.3 CENTRIFUGATION PROCEDURES

6.3.1 Temperature Requirements

Optimum results by the QBC method are obtained when the temperature of the laboratory area in which QBC tubes are prepared and tested is maintained between 20" to 32" C (68" to 90" F). To avoid excessive heating of blood tubes during the centrifugation step, keep the QBC Centrifuge out of direct sunlight.

6.3.2 Loading and Balancing the Head

Insert the open end of each blood tube under the flange of the head nut, then lower the tube into the head slots (see Figure4). When centrifuging an odd number of tubes, always balance the head with an empty QBC tube fitted with a closure and float.

Be sure to maintain proper identification of specimens when balancing the head.

6.3.3 Centrifugation Procedure

a. Secure the metal cover over the loaded head; close and latch the lid.

b. Lightly press the white button to start the centrifugation cycle.

c. After centrifugation is complete and the head stops, PROMPTLY REMOVE THE QBC TUBES FROM THE CENTRIFUGE.

I MPOaTANT

FAILURE TO REMOVE QBC TUBES PROMPTLY AFTER CENTRIFUGING MAY DISTURB THE EXPANDED CELL LAYERS AND AFFECT SUBSEQUENT TEST RESULTS.

d. Place the centrifuged tubes in a vertical closure-down position in the QB.C Work Station as shown in Figure 9. NOTE: When stored as directed, QBC Tubes can be iested in the QBC Reader any time within four ( 4 ) hours after centrifuging.

Figure 9. QBC Work Station Showing Tube Storage Slots.

e. Read QBC Tubes according to detailed ~nstructions in the Package Insert or applicable QBC Operator's Manual.

I

CAUTION

TEST VALUES FROM QBC BLOOD TUBES CAN ONLY BE OBTAINED ON A QBC SERIES READER INSTRU- MENT. DO NOT ATTEMPT TO READ QBC TUBES ON CENTRIFUGE SCALES OR WITH MECHANlCAL TUBE- READING DEVICES.

Section 7 TEST RESULTS AND PERFORMANCE

7.1 TEST RESULTS

~ e s t ' results by the QBC method are obtained by measuring the packed cell layers of the centrifuged blood tube in a QBC-series Reader Instrument.

8' 7.2 LIMITATIONS OF THE QBC PROCEDURE

To obtain test results by the QBC method, a special QBC Tube Reader instrument is required to measure the hematocrit and expanded cell layers of the buffy coat. As described in the applicable QBC Operator's Manual, test results cannot be obtained unless clearly differentiated cell layers are present in the blood tube.

7.3 EXPECTED VALUES

Consult the Package Inserts and QBC Operator's Manual for information on normal values of QBC test parameters.

7.4 SPECIFIC PERFORMANCE CHARACTERISTICS

When used in conjunction with the QBC Series Centrifugal Hematology System, the QBC Centrifuge wilt yield packed cell volumes for the deter- mination of hematocrit, platelet count, whita cell count and counts of granulocytes and lymphocytedmonocytes. Data on precision and accuracy of the System are contained in the Package Inserts and applicable QBC Operator's Manual.

Appendix A PARTS REPLACEMENT PROCEDURES

Should the Centrifuge fail to operate, first check the condition of the line fuse and replace if necessary, as described below in Paragraph 1.

If the fuse is not blown, the motor brushes are most likely worn, rendering the motor inoperable. Replacement procedures for the motor brushes are described in Paragraph 2.

WARNING

TO AVOID ELECTRICAL SHOCK, ALWAYS DISCONNECT THE POWER CORD BEFORE PERFORMING THE FUSE AND BRUSH REPLACEMENT PROCEDURES fN PARAGRAPHS 1 AND 2.

A

1. LINE FUSE

With the power cord connected to the wall receptacle, a quick check of the line fuse can be made as follows: press the PUSH-TO-START/PUSH-TO- STOP Button switch once. If the Button lights but the Centrifuge motor fails to operate, the fuse is good. Proceed to replacement of the MOTOR BRUSHES in Paragraph 2.

I f the button does not light and the motor does not operate, the line fuse located on the Timer Circuit Board is blown. To replace the fuse:

a. DISCONNECT THE POWER CORD FROM THE WALL RECEPTACLE.

b. Close the lid and turn the Centrifuge over.

c. Remove the bottom cover (with attached rubber feet) by removing the three Screws and Lockwashers (Figure A-1 ) that secure the cover to the base of the housing. DO NOT REMOVE THE RUBBER FEET.

SCREWS (31

LOCK WASHERS (3)

BOTTOM COVER

Figure A- 7 . Location o f 80 ttom Cover Screws.

TIMER CIRCUIT BOARD

FUSE-

'IRE ONN

Figure A-2. Fuse Location and Wire Connection Details.

d. Referring to Figure A-2, carefully pry off as many as necessary of push-on Wire Connectors from the Timer Circuit Board to gain access to the clip-mounted Line Fuse.

e. Remove the Fuse from its Spring Clips and snap in a correct replace- ment fuse as follows:

Centrifuge Model No. Votts/Frequency Fuse Rating

4207 120V/60 Hz 4 Arnp/3AG Slo-Blo* 4208 100V/50or60Hz 4Amp/3AGSlo-Blo* 4209 220V/50Hz 2 Amp/3AG Slo-Bto*

'Littlefuse Type 31 3

CAUTION

TO AVOID FIRE HAZARDS, BE SURE TO INSTALL THE REPLACEMENT FUSE SPECIFIED ABOVE AND ON THE DATA PLATE OF THE CENTRIFUGE.

f. Replace all push-on Wire Connectors to their correct terminals on the Timer Circuit Board as shown in Figure A-2.

g. Replace the bottom cover, lockwashers and cover screws, then turn the Centrifuge over.

h. Re-connect the power cord plug and check for correct operation of the Button Light and motor.

IMPORTANT

1F THE REPLACEMENT FUSE BLOWS OUT, REQUEST TECHNICAL ASSISTANCE TO CORRECT AN INTERNAL SHORT CIRCUIT.

$9 2. MOTOR BRUSHES

a. Removal I

To remove the Motor Brushes: I

1. DISCONNECT THE POWER CORD PLUG FROM THE WALL RECEPTACLE.

2. Remove the rotor head by reversing the installation procedures in Section 3.1. IMPORTANT: Also remove the Motor Shaft Adapter

I I

from the Motor Shaft (see Figure 3 in Section 3.1).

3. Close the lid and turn the Centrifuge over.

4. Remove the bottom cover as described in Step (cj under Paragraph 1. 5. With a Phillips-head screwdriver, loosen and remove the three motor

mounting Screws (A) from the three motor Ballmounts (8). See Figure A-3 for details on the following ballmount hardware: lock- washers, 2 flatwashers, and bushing.

OUTER BRUSH CAP VIOLET WIRE

MOTOR SUPPORT BRACKET

JND WlRE

Figure A-3. Motor Ballmount Attaching Hardware.

SNAP-OUT CAP^ (FAR SIDE) -1

A'.' SNAP-OUT CAP

Figure A-4. Location of Snap-Out Caps in Motor Enclosure.

6. Lift up the motor (with attached support bracket and ballmounts) until the two snap-out Caps on both sides of the motor enclosure are easily accessible (Figure A-4). NOTE: It is not necessary to dis- connect any wire leads to the motor to gain access to the motor brush caps.

7. With the blade of a flat screwdriver, pry off the two Caps.

8. Unscrew the slotted Brush Caps inside the motor enclosure. The Brush Contact Plate should spring out when the Brush Cap is removed. If not, carefully pry out with a small screwdriver to remove the brush assembly (Figure A-5).

- BRUSH CI.."..,,

I un \ BRUSH 7

"" SNAP-OUT dfCAP Figure A-5. Brush Assembly Removal.

b. Installing New Motor Brushes ,

To install new Part No. 4457-603-003 motor brushes:

I . Orient the curved surface of each brush so that it matches the curva- ture of the motor housing as shown in Figure A-6.

CURVED MOTOR SURFACE

HOUSING

BRUSH

Figure A-6. Correct Orientation o f New Motor Brushes.

2. Insert the brush, press the contact plate and spring in, and re-install the threaded Brush Cap. Repeat for the other brush.

3. Replace the two outer Caps on the motor enclosure.

4. Referring to Figure A-7, be sure the large washers are in position over the three motor mounting pads. Correctly orient the motor and support bracket. Gently lower the assem bly down onto the washers. NOTE: Be sure the baltmount bushings are in place and do not slip out during re-assembly.

5. Re-assemble the attaching hardware of each ballmount exactly as shown in Figure A-7. Then securely tighten the ballmount screws with a Phillips-head screwdriver.

6. Replace the bottom cover. Turn the centrifuge over. Referring to Figure 3.1, orient the Motor Shaft Adapter as shown, and re-install over the motor shaft, flanged-end facing up. Replace the head as described in the procedures in 3.1.

7. Reconnect the line cord and operate the Centrifuge through t i ree to four complete 5-MINUTE spin cycles to wear in the new brushes. DO NOT OPERATE THE CENTRIFUGE UNLESS THE 'HEAD IS INSTALLED SINCE IT MAY DAMAGE THE GOVERNOR.

LARGE WASHERS

OUTER BRUSH

RED WlRE

VIOLET WIRE

MOTOR SUPPORT BRACKET

CAP

Figure A-7. Motor and Ballmount installation Details.

3. LID-SEAL GASKET

After prolonged use, the neoprene foam rubber gasket (Figure A-8) which contacts the lip of the Centrifuge lid may lose its sealing properties and require replacement with a new Part No. 4457-600-010 gasket.

Remove the old gasket by peeling it from the groove in the housing. Scrape or rub off any residual adhesive remaining in the gasket groove.

Apply a thin bead of PERMABOND White Label Gasket Seal (or equivalent adhesive for bonding rubber material) around the bottom of the gasket groove. Orient the new gasket as shown in Figure A-8 and press firmly around the circumference of the groove. NOTE: Be sure the open space between the ends of the gasket is at the rear of the centrifuge. Allow the adhesive to dry before closing the lid.

PIN GASKET

4457-600-0 10

Figure A-8. Location of Lid-Seal Gasket.

A-6

Appendix B ILLUSTRATED PARTS LIST

Illust. No. Description

Model 4207 Centrifuge (120V, 60Hz)

1 Timer 4 * Line Fuse: 4 Amp, 3AG Slo-Blo** 6 Motor 9 Cordset Assembly

11 Capacitor, Motor 13 Resistor, Motor

Model 4208 Centrifuge (100V, 50/60Hz)

3 f imer 4 * Line Fuse: 4 Amp, 3AG Slo-Blo** 8 Motor 9 Cordset Assembly

11 Capacitor, Motor 13 Resistor, Motor

Model 4209 Centrifuge (220V, 50Hz)

2 Timer 5* Line Fuse: 2 Amp, 3AG Slo-Blo** 7 Motor

10 Cordset Assembly '. 12 Capacitor, Motor

14 Resistor, Motor

UNIVERSAL PARTS - ALL MODELS

Head Cover Assembly Rotor Head Assembly Motor Shaft Adapter Head Nut Head Nut Wrench Lid Seal Gasket Lid Latch Lid Switch Assembly Catch Plate, Lid Latch Motor Brushes: 1 Set Governor Brushes, Motor: 1 Set Cushion, Motor Motor Ballmount Kit: 3 Sets (Includes per set: ballmount, bushing, 2 ballmount washers, flatwasher, lockwasher, and screw) Feet: 1 Set

ParVReorder No.

4457-600-006 Procure Locally 4457-603-001 4457-607-000 4457-61 8-51 0 4457-61 6-01 5

4455-600-006 Procure Locally 4455-603-001 4457-607-000 4457-61 8-5 10 4457-61 6-01 5

4456-600-006 Procure Locally 4456-603-00 1 4456-607-000 4457-61 8-51 5 4457-6 1 6-0 1 6

QBC Centrifuge - Illustrated Parts Breakdown.

*User-Replaceable Part '*Littlefuse Type 313

Appendix C WIRING DIAGRAM QBC CENTRIFUGE

POWER 120V/60Hz & lOOV/ 50 Hz, 60Hz

GRD

GRD

Wire Colors in 220 Volt Model

@ = GRN/YEL

@ = LIGHT BLUE

@ = LIGHT BLUE

@ = BRN