DEPARTMENT OF BIOTECHNOLOGY, JIIT, NOIDA

Plant and Microbial Biotechnology

Research Group

Vision

Create a wealth of scientifically verified information

on natural resources

including plants and microbes.

Vision Create a wealth of scientifically verified information

On natural resources Including plants and microbes

Mission

To provide biotechnology driven solutions

For abetting developmental concerns

In Environment, Agriculture & Industrial sectors

Thrust Areas / Major Research Areas / Major Research Groups

Area / Group Name: Plant and Microbial Biotechnology

Group coordinator: Dr S Krishna Sundari Research Focus:

New advances in biotechnology are providing great insights in to the workings of nature,

presenting interesting opportunities to apply principles of biology to different fields of

science. Sustainable solutions are emerging to address the concerns on improving crop

productivity, depleting natural resources, environmental pollution, safety of food and

agricultural products etc.. Concurrently, there is an increasing demand for natural bio-

products of therapeutic and industrial importance (in the areas of healthcare,

environmental remediation, agriculture biotechnology). This has provided an impetus for

research on plants and microorganisms that produce novel bio-products with variable

properties and understanding their mechanisms of action at molecular level. Hence research

activities of the Plant and Microbial Biotechnology group at Department of Biotechnology

are comprehensively focused up on major sectors viz., bioresources, biorefining,

bioremediation of organic and inorganic pollutants, enzymes for environment, food,

industrial applications, biofertilisers, biocontrol agents for agriculture improvement and

natural products for healthcare applications. The group has garnered extra mural funding to

the tune of ~ 91 lakhs through research grants from Department of Biotechnology (DBT),

Department of Science & Technology (DST) & Department of Ayurveda, Yoga &

Naturopathy, Unani, Siddha and Homoeopathy (AYUSH), Govt. of India.

A brief over view of research in the group: Ability of native plant growth promoting microorganisms (PGPMs) are being evaluated to offer holistic plant growth benefits (providing nutritional benefits along with resistance to soil pathogens, and help reclaim agriculture soils containing residual pesticides). A consortium of PGPM is developed that can be used as bioinoculant (biofertilisers, biopesticides) to improve agriculture productivity. Ability of select PGPM to remediate organic pollutants in agriculture soils is also being explored. Biorefining, involving biocatalysts in the form of whole cell microbes or enzymes derived from native sources is being experimented with, to remove nitrogen and sulfur/aromatic content present in fossil fuels. Research comprises isolation of microorganisms capable of expressing genes involved in the degradation of contaminants present in fossil fuels. Microbes (bacteria/actinomycetes) are being screened from niche habitats (desert/hydrocarbon-polluted soil) for isolating antibiotics, biosurfactants, enzymes and are also being characterized to study taxonomic diversity. Industrial enzymes being studied to obtain improved properties for technical applications are: phytase (phosphate utilisation properties for fish/poultry feed), tannase (treatment of industrial effluents), protease, keratinase (feather degradation property for solid waste management), tannase (for tea processing, effluent treatment, juice processing) and amylase (starch desizing in textile industry). Research groups in the department are actively working on certain other important bio-products viz. food flavours (vanillin), biopolymers (resistant starch from elephant foot and chitosan from fungi, bacterial cellulose), antibiotics and other by-products (gallic acid). Probiotic formulations are being developed using novel microorganisms and those exhibiting resistance to gastric digestion. New structurally diverse natural products of industrial importance (healthcare - drugs, and environmental remediation- enzymes, biosurfactants) are being studied to address the need for newer molecules with better target profiles. Natural products from plants are being evaluated for respiratory diseases and as immune boosters for asthma and diabetic conditions as well as for neurological conditions and for anti-oxidative activity. Isolation and identification of microorganism for the bioremediation of sites contaminated with poly-aromatic compounds is being studied. Microbial (ex. Pseudomonas putida) ability for tertiary treatment of paper mill effluent has been studied by applying sequential treatment composed of two-step chemical precipitation in order to meet discharge limits for various environmental contaminants. Project related to bacterial surface display of outer membrane protein in E. coli to harvest metal ions from the environment is in progress.

PLANT AND MICROBIAL BIOTECHNOLOGY Facilities / Infrastructure

S.No. Name of equipments& numbers Make/company

1 BOD Incubator Hicon 2 Cold room Blue star 3 Spectronic( 20d+ ) Thermospectronic 4 Monocular microscope Olympus 5 Autoclave Hicon, Atlantis 6 Laminar flow (horizontal) S.m. International 7 Micropippetes Eppendorf 8 Centrifuge (refrigerated) -3k30 Sigma 9 Gel documentation system Bio-rad 10 Gel drying system Bio rad 11 Elisa reader model -benchmark Bio-rad 12 Water bath Gfl, Germany 13 Incubator shaker Kuhner 14 Thermal cycle (PCR) Bio rad 15 Electronic analy. Balance Denver 16 Conductivity meter model - 145a+ Thermo Orion 17 pH meter model - 420a+ Thermo Orion 18 Comprehensive plant tissue culture lab Vista biocell 19 pH meter digital Elico 20 Fermentor (7 ltrs) Bioage 21 Deep freezer -80 model - u410+ New brunswick 22 Lyophilizer model - alpha 1-2ld Christ 23 HPLC model- water -2996 Waters 24 BOD incubator Narang sci. System 25 Binocular microscope ch20i Olympus 26 Digital incubator orbital shaker Macflow 27 Digital circular chillar bath Macflow 28 Digital water bath with incubator shaker Macflow 29 High precision balance - cy510c Citizen 30 Balance model - CTG 602 Macflow 31 Binocular microscope with photo interface -bx51 Olympus 32 Magnus zoom trinocular microscope model- msz- Olympus 33 Olympus zoom binocular microscope model- sz51- Olympus 34 Magnus zoom binocular microscope model-msz Olympus 35 Thermal cycler (PCR) model- peltier Bio-rad 36 Incubator shaker model -lab thermlt-x Kuhner 37 Laminar flow Atlantis 38 Biosafety hood Atlantis india 39 Refrigerator- (Sanyo ) Sanyo 40 Universal frequency counter,power supply Bharti electronics 41 Premium upright freezer, model no -u410 New Brunswick 42 Chemical storage cabinet (model-csc-pp-40-24-87) 45 Atlantis India 43 Laminar flow vertical -model v-42 Atlantis India 44 Remi cooling microfuge -cm-12 -, microcentrifuge -1020 Remi 45 UV-Vis spectrophotometer model UV-1800 Shimadtzu 46 Gas chromatography Thermo scientific

Annexure-BT1

PLANT AND MICROBIAL BIOTECHNOLOGY Research Grants

Research efforts of the group reflects in sponsored research grants of Rs.124.67 Lakhs from premier funding agencies of Govt. of India namely: Department of Biotechnology (DBT), Department of Science & Technology (DST), Department of Ayurveda, Yoga & Naturopathy, Unani, Siddha and Homoeopathy (AYUSH) and Industry sponsored project. Research projects: 1. Formulation of Microbial consortia with parallel biofertilizer and biocontrol properties. (DBT): PI: Krishna Sundari, Co-PI: Dr Reena Singh (TERI). (2010-2014) Total grant value: Rs. 57.39 Lakhs, (JIIT: Rs. 24.22 Lakhs, TERI: 33.17 Lakhs). (Completed) 2. Development of a biocatalyst for dearomatization of diesel. (DBT), PI: Nidhi Gupta, Co-PI: Sanjay Gupta, Co-PI: D.K. Adhikari (Indian Institute of Petroleum, Dehradun) (2013-2014). Grant value: Rs. 6.92 Lakhs. (Completed) 3. Ability of select PGPM strains to remediate organophosphate pesticides commonly used in agriculture. (DBT) PI: Krishna Sundari (2013-2015) Grant value: Rs. 6.59 Lakhs. (Ongoing) 4. Scientific documentation (digitization) of the selected Indian Medicinal Plants used for antidiabetic and other activities. AYUSH-NMPB-MHRD, PI: Dr Rachana, (2009-2011) Grant Value: Rs. 7 Lakhs. (completed) 5. Development of a biocatalyst for the removal of nitrogen and sulfur from diesel: (DST), PI: Nidhi Gupta (2013-2016). Grant value: Rs. 24.9 Lakhs. (Ongoing) Industry Sponsored Project: 1. Biotechnological solution for attaining longer shelf life and portability of microbial inoculum. Funded by Trident GreenTech Pvt. Ltd., Andhra Pradesh. PI: Dr. S Krishna Sundari (August 2015 – June 2016); Grant value: Rs. 19 Lakhs approx. (Ongoing) Women Scientist Project (DST-WoS-A) 1. Studies on production of therapeutically important saponins using in-vitro culture of

Bacopa monnieri. (DST) PI: Pragya Bhardwaj (2014-2017); Faculty mentors: Dr. Ashwani Mathur, Dr. Chakresh K Jain. Grant value: Rs. 16.60 Lakhs. (Ongoing)

Post Doctoral Research Associateship 1. Screening of native microbes with tannase producing ability, production of tannase and

gallic acid using alternate substrate: (DBT), PI: Krishna Sundari (Jan 2012- Dec 2015). Grant value: 19.44 Lakhs. (Ongoing)

Total research grant value: Rs. 124.67 Lakhs

Inter Institutional Collaborations

Strategic partnerships and collaborations have been established with scientists from various institutions and research centres of repute that enable exchange of research reagents and protocols and sharing specialized expertise and facilities.

Prof. Sanjay Rangnate Dhakate, Principle Scientist, Department of Materials Physics and Engineering, NPL, CSIR, New Delhi.

Dr D.K Adhikari, Chief Scientist, Biofuels Division & HOA Biotechnology Conversion Area, Indian Institute of Petroleum, Mohkampur, Dehradun.

Dr. Reena Singh, Area Convenor, CMR, TERI, Habitat place, Lodi road, New Delhi.

Prof. Rup Lal, Department of Zoology, Delhi University, Delhi.

Prof. Subhash Chand, Professor Emeritus, Department of Biochemical Engineering & Biotechnology, IIT Delhi.

Prof. J.N Chakraborty, Department of Textile Technology, National Institute of Technology, Jalandhar.

Research Achievements Some of the Research Achievements of the department in the thrust area Plant & microbial Biotechnology are:

Isolated and characterised 13 plant growth promoting isolates to be developed as bioinoculants for crop improvement.

A consortium of PGP microbes with dual biofertiliser and biocontrol abilities developed.

Isolated and characterized four different microorganisms for the degradation of carbazole.

Cloning expression & purification of enzyme dioxygenase for carbazole degradation.

Development of sequential tertiary treatment method using metal salt and microorganism for paper mill effluent.

Production of bacterial cellulose, characterization and application in bioprocessing

Production of fungal chitosan and preparation of blend membranes for industrial application.

Isolation, taxonomic identification and characterization of actinomycetes from desert; characterization of antimicrobial compounds from them.

Identification and characterization of bacteria from sodic-alkaline soil for amylase production and starch desizing

In vitro propagation of medicinal plants and evaluation of their therapeutic potential

Monographs of medicinal plants of India.

Enzyme Keratinase produced from soil isolate with proven applicability in imparting anti-shrink functionality to wool and works synergistically with other scale removing enzymes

Resource Development: The department contributes in the research training of undergraduate and graduate students through both vertical and horizontal integration of research projects in Ph.D, M.Tech and B.Tech programs. Degree has been awarded to PhD (4), M.Tech (55) and B.Tech (226) students who have pursued research in this thrust area. Presently the faculty in the area are actively involved in scientific engagements where research projects at post doctoral, doctoral training are presently ongoing: Post doc (1), PhD (12), M.Tech (20) and B.Tech (24). Well-trained undergraduates and postgraduates students have gone for their MS or PhD degrees to Universities of National and International repute such as: University of Pennisylvania (Ashmita Saigal); Pennsylvania State University (Manmeet S Dayal); Yale (Anumeha Shah), University of southern California (Ambika ramrakhiani), North Carolina (Tanya Sabharwal), University of Cincinnati (Vikram Kapoor), New York University (Rohan Seth), Kent university (Aanchal Khadelwal), University College London (Radhika Chadda); NYU Polytechnic School of Engineering (Sneh Sharma), Center Ambedkar Centre for Biomedical Research (Manal Shakeel), IITs (Shivani Bisht, Richa Nigam, Sharadwata Pan), NITs (Sakshi), TERI-SAS (Rahul Singh, Anushka Jain), BITS (Neha Panjiar), ICT, Mumbai (rohan Chabbra). Some of the students secured employment / research position with reputed biotech/biopharma industries such as: U.S. Environmental Protection Agency (Vikram Kapoor), Memorial Sloan Kettering Cancer (Sneh Sharma), Novartis Health care pvt. Ltd.(Abhishek Neeraj), Zydus Cadila (Siddharth Bichpuria), Pall Life sciences (Prashant Kishore), Reddy labs (Siddharth Bichpuria), TERI (Madhusmita, Rahul), Panacea Biotec (Geetanjali Menia, Shambhavi Seth, Priyanka Gupta, Tanya Sabharwal), Premas Biotech (Priyo Kumar) Biocon (Rajat Goyal). Some students have taken up the path of entrepreneurship and established their own start ups: Gralit India Biotech Pvt. Ltd (Gaurav Kumar, Atul Kumar).

PLANT & MICROBIAL BIOTECHNOLOGY

Group Members Faculty Area of Specialization

Dr. Neeraj Wadhwa Enzyme technology

Dr. S Krishna Sundari Bioactive products, Environmental, Agriculture Biotechnology

Dr. Indira P Sarethy Bioresource, Natural products

Dr. Nidhi Gupta Environmental Biotechnology

Dr. Susinjan Bhattacharya Microbial biotechnology

Dr. Rachana Natural products

Dr. Ashwani Mathur Bioprocess Engineering, Natural products

Dr. Pammi Gauba Environmental Biotechnology

Dr. Smriti Gaur Microbial Biotechnology

Dr. Garima Mathur Environmental Biotechnology, Natural products

Annexure BT-2

PLANT & MICROBIAL BIOTECHNOLOGY Publications

International Journal: 94 1. S.Khan, D.K. Adhikari, S.Gupta, N.Gupta, “High -level Expression, purification and

characterization of carbazole dioxygenase, a three components dioxygenase, of Pseudomonas GBS.5” Biotechnology Letters, 2015, Doi: 10.1007/s10529-015-1876-3 [Indexed in Scopus, Impact Factor:1.7]

2. S. Dangayach, P. Sharma, P. Singhai, N. Gupta, “Microbial removal of arsenic: Mechanisms and Applications” Asian Journal of Multidisciplinary studies, Vol 2, pp. 159 – 170, 2014.

3. Yadav, P. and Sundari, S. Krishna. “Plant growth promoting rhizobacteria: An effective

tool to remediate residual organophosphate pesticides applied principally in agriculture

soils”. Journal of Environmental Research and Development. Vol. 9(4), 1138-1149, 2015.

4. Mathur, G., Dua, A., Das, A.R., Kaur, H., Kukal, S., Sharma, P., Goswami, N., Sahai, A. and

Mathur, A. “Bacterial cellulose: Biopolymer from Gluconacetobacter xylinus”.

Macromolecular Symposia. Vol. 347, pp. 27-31, 2015. [Indexed in Scopus, Impact factor:

0.913].

5. Prakash, A., Verma, A., Goyal, S. and Gauba P. “Remediation of Antibiotics from the

Environment”. Journal of Basic and Applied and Engineering Research. Vol. 2(8), pp 632-

636, 2015.

6. Goyal, S., Prakash, A., Verma, A. and Gauba P. “Remediation of heavy Metals. Journal of

Basic and Applied and Engineering Research. Vol. 2(9), pp. 727-729, 2015.

7. Basu, S, Pant, M. and Rachana. "Protective effect of Salacia oblonga against tobacco

smoke-induced DNA damage and cellular changes in pancreatic â-cells". Pharmaceutical

biology pp. 1-7, 2015.

8. Sundari, S. Krishna. and Potapragada, H.S. “Bioelectronics: Revolutionizing the research

landscape of modern medicine, security and environmental applications”. Advanced

Research in Electrical and Electronic Engineering. Vol. 10(2), pp. 97-101, 2015.

9. Sundari, S. Krishna., Kotiyal S, Singhai S and Gupta N. “Evaluation of antimycotic activity

of Eucalyptus globules, Datura stramonium and Tagetes patula against three

economically important plant pathogens”. Journal of Environmental Research and

Development. Vol. 9(3A), pp.762-772, 2015.

10. Mishra, N. and Sundari, S. Krishna. “Native PGPM Consortium: A Beneficial Solution to

Support Plant Growth in the Presence of Phytopathogens and Residual

Organophosphate Pesticides”. Journal of Bioprocessing and Biotechnology. Vol. 5(2),

pp. 1-8, 2015. doi:10.4172/2155-9821.1000202

11. Sharma, P., Mathur, G., Goswami, N., Sharma, S. K., Dhakate, S. R., Chand, S. and

Mathur, A. “Evaluating the potential of chitosan/poly(vinyl alcohol) membranes as

alternative carrier material for proliferation of Vero cells”. e-Polymers. (DOI

10.1515/epoly-2015-0021) 2015.

12. Yadav, T., Mishra, S., Das, S., Aggarwal, S. and Rani, V. “Anticedants and natural

prevention of environmental toxicants induced accelerated aging of skin”.

Environmental Toxicology and Pharmacology. Vol. 9(1), pp.384-391, 2015.

13. Gauba, P. “Lactose Intolerance –A Review”. Current Nutrition and Food Science Vol.

11(3), pp. 209-212, DOI: 10.2174/1573401311666150514231452.

14. Singh, A., Gupta, P., Shukla, G. and Wadhwa, N. “Quality attributes and acceptability of

bread made from wheat and Amorphophallus paeoniifolius flour”. Journal of Food

Science and Technology. 2015. DOI 10.1007/s13197-015-1834-z [Indexed in Scopus,

Impact factor: 2.024].

15. Singh, A., Gupta, P. and Wadhwa, N. “Cellulase from stored Amorphophallus

paeoniifolius in clarification of apple juice”. International Food Research Journal. Vol.

22(2), pp. 847-850, 2015. [Indexed in Scopus].

16. Shakeel, M., Ghura, S., Gaur, S. and Gauba, P. “Mercury Neurotoxicity: a review of

case”. Asian Journal of Multidisciplinary Studies. Vol. 3(1), pp. 9-16, 2015.

17. Mathur, G., Dua, A., Das, A.R., Kaur, H., Kukal, S., Sharma, P., Goswami, N., Sahai, A. and

Mathur, A. “Bacterial cellulose: Biopolymer from Gluconacetobacter xylinus”.

Macromolecular Symposia. Vol. 347, pp. 27-31, 2015. [Indexed in Scopus, Impact factor:

0.913].

18. Mehndiratta, P., Jain, A., Singh, G.B., Sharma, S., Srivastava, S., Gupta, S. and Gupta, N.

“Magnetite nanoparticle aided immobilization of Pseudomonas sp. GBS.5 for carbazole

degradation”. Journal of Biochemical Technology. Vol. 5(4), pp. 823-825, 2014. [Indexed

in Scopus].

19. Sarethy, I.P., Kashyap, A., Bahal, U., Sejwal, N. and Gabrani, R. “Study of liquid culture

system for micropropagation of the medicinal plant Solanum nigrum L. and its effect on

antioxidant property”. Acta Physiologiae Plantarum. DOI 10.1007/s11738-014-1655-0,

2014. [Indexed in Scopus Impact factor: 1.732].

20. Nandini S., Nandini, K.E. and Sundari, S. Krishna. Food and agriculture residue (FAR): A

potential substrate for tannase and gallic acid production using competent microbes.

Journal of Bioprocessing and Biotechniques. Vol. 5(1), pp. 1-8. 2014.

21. Singh, A., Gupta, P. and Wadhwa, N. “Properties of cellulolytic enzymes from peel of

Amorphophallus paeoniifolius”. International Journal of Pharmacy and Pharmaceutical

Sciences. Vol. 6(4), pp. 333-336, 2014. [Indexed in Scopus, Impact factor: 0.91].

22. Mathew, A., Verma, A. and Gaur, S. An in-silico insight into the characteristics of β-

propeller phytase, Interdisciplinary Sciences: Computational Life Sciences. Vol. 6 pp.

133–139, 2014. [Indexed in Scopus, Impact factor: 0.672].

23. Sharma, G., Raturi, K., Dang, S., Gupta, S. and Gabrani, R. “Combinatorial antimicrobial

effect of curcumin with selected phytochemicals on Staphylococcus epidermidis”.

Journal of Asian Natural Products Research. Vol. 16(5), pp. 535-541, 2014. [Indexed in

Scopus, Impact factor: 0.97].

24. Chhabra, R., Sachdeva, A., Mathur, G., Sharma, P., Goswami, N., Jain, C.K., Sharma, S.K.

and Mathur, A. “Enhanced production of fungal chitosan from Aspergillus niger using

statistical optimization”. Journal of Chitin and Chitosan Science. Vol. 2, pp. 1-5, 2014.

25. Gahlawat, S, Makhijani, M., Chauhan, K., Valsangkar, S. and Gauba, P.”Accessing the

phytoremediation potential of Cicer arietinum for Aspirin” International Journal of

Genetic Engineering and Biotechnology. Vol. 5(2), pp. 161-168, 2014.

26. Makhijani, M., Gahlawat, S., Chauhan, K., Valsangkar S. and Gauba, P.

“Phytoremediation potential of Cicer arietinum for tetracycline”. International Journal of

Genetic Engineering and Biotechnology. Vol. 5(2), pp. 153-160, 2014.

27. Aggarwal, P., Gaur, S. and Gauba, P. “Neurotoxic and genotoxic effects of methyl

mercury”. Environment, Development and Sustainability-Springer. Vol. 16(1), pp. 71-78,

2014.

28. Singh, A. and Wadhwa, N. “Review on Multiple Potential of Aroid: Amorphophallus

paeoniifolius”. International Journal of Pharmaceutical Sciences Review and Research.

Vol. 24(1), pp. 55-60, 2014.

29. Basu, S., Pant, M. and Rachana. “In vitro antioxidant activity of methanolic-aqueous

extract powder (root and stem) of Salacia oblonga”. International Journal of Pharmacy

and Pharmaceutical Sciences. Vol. 5(3), pp. 904-909, 2013.

30. Basu, S., Pant, M. and Rachana. “Anti-oxidant activity and cytoprotective potential of

ethanolic extract of Adhatoda vasica” International Journal of Pharmaceutical Sciences

Review and Research. Vol. 5(2), pp. 501-510, 2013.

31. Mathur, G., Roy, N. and Mathur, A. “In vitro analysis of Aegle marmelos leaf extracts on

skin pathogens.” Journal of Applied Pharmaceutical Science, Vol. 3(10), pp. 97-100,

2013. (Indexed in SCOPUS)

32. Pan, S., Neeraj, A., Srivastava, K.S., Kishore, P., Danquah, M.K. and Sarethy, I.P. “A

Proposal for a Quality System for Herbal Products”. Journal of Pharmaceutical Sciences,

Vol. 102(12), pp. 4230-4241, 2013. [Indexed in SCOPUS, Impact factor 3.13]

33. Chanda, S., Sarethy, I.P., De B. and Singh, K. “Paederia foetida - a promising ethno-

medicinal tribal plant of northeastern India”, Journal of Forestry Research. pp. 1-8, 2013.

34. Singh, G.B., Gupta, S. and Gupta, N. “Carbazole degradation and biosurfactant

production by newly isolated Pseudomonas sp. strain GBS.5,” International Journal of

Biodeteoration and Biodegradation. Vol. 84, pp. 35-43, 2013. [Indexed in SCOPUS,

Impact factor: 2.059]

35. Panjiar, N., Gabrani, R. and Sarethy, I.P. “Diversity of biosurfactant-producing

Streptomyces isolates from hydrocarbon-contaminated soil”. International Journal of

Pharma and Bio Sciences. Vol. 4(1), pp. 524-535, 2013. [Indexed in SCOPUS, Impact Factor

0.4]

36. Dayal, M.S., Goswami, N., Sahai, A., Jain, V., Mathur, G. and Mathur, A. “Effect of media

components on cell growth and bacterial cellulose production from Acetobacter aceti

MTCC 2623”. Carbohydrate Polymer. Vol. 94, pp. 12-16, 2013. (Impact Factor: 3.628)

37. Singh, A., Srivastava, K.C., Banerjee, A. and Wadhwa, N. “Phytochemical analysis of peel

of Amorphophallus paeoniifolius”. International Journal of Pharma and Biosciences. Vol.

4(3), pp. 810-815, 2013.

38. Mehndiratta, P., Jain, A., Srivastava, S. and Gupta, N. “Environmental Pollution and

Nanotechnology,” Environment and Pollution”, Vol. 2, pp. 49-58, 2013.

39. Basu, S., Pant, M. and Rachana. “Phytochemical evaluation and HPTLC profiling of

extract of Salacia oblonga,” International Journal of Pharmaceutical Sciences and

Research. Vol. 4(4), pp. 1409-1418, 2013. [Impact factor-0.9]

40. Pant, M., Basu, S. and Rachana. “Protection against cytotoxicity due to tobacco smoke

by Adhatoda vasica and vasicine,” Journal of Pharmaceutical Technology Research and

Management. Vol. 1, pp. 81-88, 2013.

41. Pan, S., Neeraj, A., Srivastava, K.S., Kishore, P. and Sarethy, I.P. "Effects of growth

regulators on in vitro response and multiple shoot induction in some endangered

medicinal plants. OA Biotechnology. Vol. 2(1). 2013.

42. Pathak, G. and Rachana. Regulatory and Pharmacovigilance of Biosimilars medicinal

products”. The Pharma Review. Vol.11(65), pp. 44-47, 2013.

43. Pant, M., Basu, S. and Rachana. “Toxic effects of Indian tobacco rolls (Bidi) and beneficial

role of vasicine on mitochondrial localization and antioxidant enzymes activity in A549

cell line”. International journal of Biotechnology and bioengineering research. Vol. 4(5),

pp. 273-280, 2013.

44. Thakur, S. and Rachana. “Antioxidants: Futuristic therapeutics in the field of diabetic

neuropathy”. International journal of Biotechnology and bioengineering research. Vol. 4,

pp. 313-320, 2013.

45. Basu, S., Pant, M. and Rachana. “Beneficial effects of Salacia oblonga on mitochondrial

localization in cells and NADPH oxidase activity in glucose induced cytotoxicity on rat

muscle cell line”. International Journal of Biotechnology and bioengineering research.

Vol. 4, pp. 321-328, 2013.

46. Rana, R., Mathur, A., Jain, C.K., Sharma S.K. and Mathur, G. Microbial Production of

Vanillin. International Journal of Biotechnology and Bioengineering Research. Vol. 4, pp.

227-234, 2013.

47. Mathur, G., Nigam, R., Jaiswal, A. and Kumar, C. Bioprocess Parameter Optimization for

Laccase Production in Solid State Fermentation. International Journal of Biotechnology

and Bioengineering Research. Vol. 4, pp. 521-530, 2013.

48. Mathur, G., Mathur, A., Sharma, B.M. and Chauhan, R.S. Enhanced production of

laccase from Coriolus sp. using Plackett–Burman design. Journal of Pharmacy Research.

Vol. 6(1), pp. 151-154, 2013.

49. Dhup, S., Thakur, I., Mathur, G., and Mathur, A., “An alternative substrate for laccase

production from Pleurotus sp.,” Journal of Bioprocess Technology. Vol. 98, pp. 233-239,

2013.

50. Gupta, P., Singh, A., Shukla, G. and Wadhwa, N. “Bio-insecticidal potential of amylase

inhibitors”. Journal of Pharmacy research / BioMed RX. Vol. 1(5), pp. 449-458, 2013.

51. Shaheen, S. and Sundari S. Krishna. Exploring the applicability of PGPR to remediate

residual organophosphate and carbamate pesticides used in agriculture fields.

International Journal of Agriculture and Food Science Technology. Vol. 4(10), pp. 947-

954, 2013.

52. Nandini, K.E., Gaur A. and Sundari, S. Krishna. The suitability of natural tannins from

food and agricultural residues (FAR) for producing industrially important Tannase and

Gallic acid through microbial fermentation. International Journal of Agriculture and Food

Science Technology. Vol. 4(10), pp. 999-1010, 2013.

53. Mishra, N. and Sundari S. Krishna. Native PGPMs as bioinoculants to promote plant

growth: Response to PGPM inoculation in principal grain and pulse crops. International

Journal of Agriculture and Food Science Technology. Vol. 4(10), pp. 1055-10664, 2013.

54. Sundari S. Krishna. Medicinal value of edible ectomycorrhizal fungi; potential example

of sustainable resource utilization. Mycorriza News. Vol. 25(3), pp. 20-26, 2013.

55. Bhatia, S., Rachana, Bansal, P. and Mani, S. “Mitochondrial diabetes: Different

diagnostic features and its possible management”. Journal of International Medical

Sciences Academy. 2013.

56. Malik, S., Singh, M. and Mathur, A. “Antimicrobial activity of food grade glucosamine’.

International Journal of Biotechnology and Bioengineering Research. Vol. 4, pp. 307-312,

2013.

57. Agrahari, S. and Wadhwa, N., “Isolation and Characterization of Feather Degrading

Enzymes from Bacillus megaterium SN1 Isolated from Ghazipur Poultry Waste Site”.

Applied Biochemistry and Microbiology. Vol. 48(2), pp. 175–181, 2012. [Impact factor:

0.704].

58. Kumara Swamy, N., Singh, P. and Sarethy, I.P. “Color and phenols removal from paper

mill effluent by sequential treatment using ferric chloride and Pseudomonas putida”,

International Journal of Pharma and Bioscience. Vol. 3(2), pp. 380-392, 2012.

59. Sharma, A., Gupta, S., Sarethy, IP., Dang, S. and Gabrani, R. “Green tea extract: possible

mechanism and antibacterial activity on skin pathogens”. Food Chemistry. Vol. 135(2),

pp. 672-675, 2012. [Impact factor: 3.655].

60. Sundari, S. K. “A New Edition of an Old Favorite. Review of: Molecular Biotechnology—

Principles and Applications of Recombinant DNA”. Journal of Microbiology Education,

Vol. 13(1), pp. 101-102, 2012.

61. Sarethy, I.P., Saxena, Y., Kapoor, A., Sharma, M., Seth, R., Sharma, H., Sharma, S.K. and

Gupta S. Amylase produced by Bacillus sp. SI-136 isolated from sodic-alkaline soil for

efficient starch desizing. Journal of Biochemichal Technology. Vol. 4(1). 2012 [Impact

Factor 0.9].

62. Singh, M., Mathur, G., Jain, C. K. and Mathur, A. Phyto-pharmacological Potential of

Ginkgo biloba: a Review, Journal of Pharmacy Research. Vol. 5(10), pp. 5028, 2012.

63. Singh, A. and Wadhwa, N. “Osmotic dehydration of Amorphophallus paeoniifolius slices

and it’s phyto-chemical investigation”. International Journal of Pharmacy and Life

sciences. Vol. 3, pp. 1797-1801, 2012.

64. Gaur, S., Maheshwari, S.K. and Gauba, P., "Transgenic Plants: factories for the

production of biomedicines”. Journal of Pharmacy Research. Vol. 5(9), pp. 4856-4859,

2012.

65. Gaur, S., Gauba, P., Maheshwari, S.K. and Rachana. "Transgenic plant production

technology: Present and Future Prospective". Pharma Review. Vol. 10(55). 2012.

66. Singh, G.B., Gupta, S., Srivastava, S. and Gupta, N., “Biodegradation of Carbazole by

Newly Isolated Acinetobacter spp.,” Bulltein of Environmental Contamination and

Toxicology. Vol. 87(5), pp. 522 – 526, 2011. [Impact factor: 1.139].

67. Singh, G.B., Srivastava, A., Saigal, A., Aggarwal, S., Bisht, S., Gupta, S., Srivastava, S. and

Gupta, N., “Biodegradation of carbazole and dibenzothiophene by bacteria isolated from

petroleum contaminated sites”. Bioremediation Journal. Vol. 15(4), pp. 189 – 195, 2011.

68. Jain, R., Sharma, A., Gupta, S., Sarethy, I.P. and Gabrani, R. “Solanum nigrum: Current

perspectives on therapeutic properties”. Alternative Medicine Review. Vol. 16, pp. 78-

85, 2011. [Impact factor: 3.52].

69. Sarethy, I. P., Gulati, N., Bansal, A., Gupta, V., Malhotra, K. and Gabrani, R. “Genetic

structure of an endangered Cycas revoluta using RAPD markers”. Research Journal of

Biotechnology. Vol. 6, pp. 50-55, 2011.

70. Sarethy, I. P., Saxena, Y., Kapoor, A., Sharma, S., Sharma, S. K., Gupta, V. and Gupta, S.

“Alkaliphilic bacteria: applications in industrial biotechnology”. Journal of Industrial

Microbiology Biotechnology. DOI 10.1007/s10295-011-0968-x. [Impact factor: 2.1]

71. Kumara Swamy, N., Singh, P. and Sarethy, I. P. “Precipitation of phenols from paper

industry wastewater using ferric chloride”. Rasayan Journal of Chemistry. Vol.4(2), pp.

452-456, 2011. [Impact factor: 0.4]

72. Kumar, P.M., Saluja, S., Pant, M., Rachana and Jain, C.K. Docking Studies to Investigate

Interactions of Vasicine Molecule with Oxidative Enzymes. Journal of Pharmacy

Research. Vol. 4(11), pp. 3907-3909, 2011. [Impact factor 2.36]

73. Wadhwa, N., Asawa, K. and Agrahari, S. “Response Surface Methodology and Resilient

Back Propagation Based Yield Prediction of Protease from Bacillus Megaterium SN1”.

Journal of Pharmacy Research”. Vol. 4(3), pp. 929-932, 2011. [Impact factor 2.36]

74. Kaushik, P., Batra, E., Juneja, N., Tushar, A., Kohli, S., Suchit, A., Agrahari, S., Rani, V. and

Wadhwa, N. “Phytochemical screening of developing garlic and effect of its aqueous

extracts on viability of cardiac cell line: A comparative study” Journal of Pharmacy

Research. Vol. 4(3), pp. 902-904, 2011. [Impact factor 2.36]

75. Dogra, D., Ahuja, S., Krishnan, S., Kohli, S., Anand, R. and Rani, V. “Phytochemical

screening and antioxidative activity of aqueous extract of Indian Camellia sinensis”.

Journal of Pharmacy Research. Vol. 4(6). 2011. [Impact factor 2.36]

76. Rachana., Basu, S., Pant, M., Kumar, M. P. and Saluja, S. “Review and future perspectives

of using Vasicine, and related compounds”. Indo Global Journal of Pharmaceutical

Sciences. Vol. 1(1), pp. 85-98, 2011.

77. Manoj, K. P., Saluja, S. and Rachana. “Phytosomes” The Pharma Review, pp. 99-103,

2011. [Indexed in Intl. Pharmaceutical Abstract, Chemical abstracts and Index

Copernicus]

78. Rachana. and Pathak, G. "Biotechnology in Pharma Sector in India". Pharma Review. Vol.

9(54), pp. 65-68, 2011. [Indexed in Intl. Pharmaceutical Abstract, Chemical abstracts and

Index Copernicus]

79. Agrahari, S. and Wadhwa, N. “Degradation of Chicken Feather a Poultry Waste Product

by Keratinolytic Bacteria Isolated from Dumping Site at Ghazipur Poultry Processing

plant”. International Journal of Poultry Science. Vol. 9(5), pp.482-489, 2010.

80. Shanker, N., Vikram, N., Tyagi, A., Gabrani, R. and Sarethy, I.P. “Study of Streptomyces

diversity in arid and semi-arid soil of India”. Journal of Pure and Applied Microbiology.

Vol. 4, pp. 687-699, 2010.

81. Agrahari, S. and Wadhwa, N. “Production of extra cellular milk clotting enzyme from

isolated Bacillus” Journal of Pharmacy Research”. Vol. 3(12), pp. 2924-2927, 2010.

[Impact factor: 1.09]

82. Basu, S. and Rachana. “IPR issues with Genetically Modified Organisms (GMOs)”. The

Pharma Review. pp. 64- 67, 2010.

83. Shah, S. and Rachana. “Development and optimization of an economic method for

quantitation of azithromycin in human plasma by tandem mass spectroscopy (LCMS/MS)

for clinical trials”. Pharma Science Monitor. Pp. 1-13, 2010.

84. Jaiswal, A., Mahajan, V., Chhabra, A. and Rachana. “Best Out of Waste: Stems Cell from

Menstrual Blood”. The Pharma Review. Pp. 67-69, 2010.

85. Gaur, S., Agrahari, S. and Wadhwa, N. “Purification of protease from Pseudomonas

thermaerum GW1 isolated from poultry waste site”. The Open Microbiology Journal.

Vol. 4, pp. 67-74, 2010.

86. Rachana., Patel, V. and Joshi, G. “Toxicity studies for antidiabetic herbal formulation: a

crude mixture (1:1:1) of Stevia rebaudiana, Andographis paniculata, and Tinospora

cordifolia. Planta Medica. Vol. 75, pp. 998, August 2009. [Impact factor 1.960]

87. Rachana., Pathak, G. and Anand, V. “Molecular diagnostics: targets and travels”. The

Pharma Review. pp. 37- 40, 2009.

88. Sarawgi, G., Kamra, A., Suri, N., Kaur, A. and Sarethy, I. P. “Effect of Strychnos

potatorum Linn. seed extracts on water samples from different sources and with diverse

properties”. Asian Journal of Water Environment and Pollution. Vol. 6(3), pp. 13-17,

2009.

89. Rachana. and Pathak, G. “Plant tissue culture in herbal medicine: A New Ray to Old

way”. The Pharma Review. pp. 38- 40, 2009.

90. Gaur, S. and Wadhwa, N. “Alkaline protease from senesced leaves of invasive weed

Lantana camara”, African Journal of Biotechnology. Vol. 7(24), pp. 4602–4608, 2008.

[Impact Factor 0.6]

91. Chhabra, R., Sachdeva, A., Sharma, P, Mathur, G. and Mathur, A. “Bioprocess parameter

optimization for improving yield of chitosan from Aspergillus sp”. Asian Chitin Journal.

Vol. 9, pp. 8, 2013.

92. Kumara Swamy, N., Singh, P. and Sarethy, I.P. “Aerobic and anaerobic treatment of

paper industry wastewater”. Research in Environment and Life Sciences. Vol. 4(4), pp.

141-148, 2011.

93. Grover, N., Singh, H., Vemuri, N. and Gupta, B. " Growth of 3T3 fibroblast on Collagen

immobilized poly (ethylene terephthalate) Fabric". Indian Journal of Fibre & Textile

Research. Vol. 35, pp. 228-236, 2010.

94. Shrivastav, A. and Srivastava, S. “Medicinal plants used worldwide for treating

diabetes”. Journal of Tropical Forestry. Vol. 26(1), pp. 14, 2010.

Gene Bank Submissions: Total 11

1. I.P. Sarethy, N. Panjiar and R. Gabrani, “16S rDNA sequence of Streptomyces isolate PN-18, capable of producing biosurfactant on complex carbon substrates,” GenBank Accession No. GQ856644, 2009.

2. I.P. Sarethy, N. Shanker, N. Vikram, A. Tyagi, and R. Gabrani, “16S rDNA sequence of Streptomyces isolate B-14, capable of growing on complex carbon substrates,” GenBank Accession No. GQ426322, 2009.

3. S. Gaur and N. Wadhwa, “16S rDNA sequence of Pseudomonas thermaerum GW1,” Genbank Accession No. GU951516, 2010.

4. G.B. Singh, S. Srivastava, S. Gupta, N. Gupta “Acinetobacter sp. enrichment culture clone Alp6 16S ribosomal RNA gene, partial sequence,” GenBank Accession No. JF828047, 2011.

5. G.B. Singh, S. Srivastava, S. Gupta, N. Gupta “Acinetobacter sp. enrichment culture clone Alp7 16S ribosomal RNA gene, partial sequence,” GenBank Accession No. JF828048, 2011.

6. I.P. Sarethy, Y. Saxena, A. Kapoor, M. Sharma, S.K. Sharma and S. Gupta “Bacillus sp. SI-136 16S ribosomal RNA gene, partial sequence,” GenBank Accession No. JN314426, 2011.

7. I.P. Sarethy, Y. Saxena, A. Kapoor, M. Sharma, S.K. Sharma and S. Gupta “Bacillus sp. SI-218 16S ribosomal RNA gene, partial sequence,” GenBank Accession No. JN314427, 2011.

8. G.B. Singh, S. Srivastava, S. Gupta, N. Gupta, “Pseudomonas sp. enrichment culture clone GBS.5 16S ribosomal RNA gene, partial sequence,” GenBank Accession No. JX193073, 2012.

9. Sundari, S. K. and Nandini, K.E., “Aspergillus carbonarius internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence,” GenBank Accession No. KM117230.1, 2014.

10. Sundari, S.K. and Nandini, S., “Citrobacter frenduii. 2.2 16S ribosomal RNA gene, partial sequence. GenBank Accession No. KM 117229.1, 2014.

11. Mishra, N., Shaheen, S. and Sattiraju, K.S., “Trichoderma harzianum. Internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence,” GenBank Accession No. KP122935.1, 2014.

Books Published

1. Gupta V.K., Tuohy M., Sharma G.D., and Gaur S. Applications of Microbial Genes in

Enzyme Technology. Nova Science Publishers, USA, 2013.

2. Rachana, Basu S, “Biochemistry (for BSc)”, Punjab Technical University: Published by

Vikas publications, Noida, 2010.

3. Rachana, Sharma, S, Basu, S, “Human physiology and anatomy” (for MSc), Punjab

Technical University: Published by Vikas publications, Noida, 2010.

4. Rachana, S. Basu, “Basics of Zoology, (for BSc.), Manonmaniam Sundaranar

University Tirunelveli, Vikas publications, Noida.

Mono Graphs submitted: to AYUSH, NMPB, Ministry of Health, Govt. of India

1. Monograph for the Indian medicinal plant Salacia reticulata

2. Monograph for the Indian medicinal Andrographis panicualta

Chapter Publications

1. Gaur S., “Natural weapons from bacteria against cancer” in Microbial Resources.

(Eds. V.K. Gupta, D. Thangdurai, G.D. Sharma) CAB International Publishers, UK, In

press, 2015.

2. Agyei D., Danquah M. K., Sarethy I.P., Pan S., Antioxidative peptides derived from

food protein, in Free Radicals in Human Health & Diseases Rani, V and Yadav, U. C.

(Eds.), Springer Publications,2015, Chapter 26, pp 417-430, 2015 ISBN 978-81-322-

2035-0.

3. Mathur A., Sharma P., Goswami N., Sahai A., Dua A., Das A.R., Kaur H., Kukal S.,

Dayal M.S., Arora S., Mishra P., Jain V. and Mathur G. Comparative studies on

production of bacterial cellulose from Acetobacter sp. and application as carrier for

cell culturing. Industrial, Medical and Environmental Applications of

Microorganisms: Current Status and Trends, Wageningen Academic Publishers,

2014, Vol. 37, issue 8, pp. 403-407.

4. Mathur A., Chhabra R., Sachdeva A., Sharma P. and Mathur G.. Fungal chitosan: a

suitable biomaterial for cell culturing. Industrial, Medical and Environmental

Applications of Microorganisms: Current Status and Trends, Wageningen Academic

Publishers, 2014, Vol. 37, issue 8, pp. 436-440,

5. S. Krishna Sundari (2014). Impact of biotic, abiotic stressors: Biotechnologies for

alleviating plant stress. In “Use of Microbes for the alleviation of salt stress”. M.

Miransari (Ed). Springer Science+Business Media New York, DOI: 10.1007/978-1-

4939-0721-2_6, Chapter 6. pp.87-120.

6. A.K. Gupta, R. Chaddha, R. Shah, S. Krishna Sundari. “Methods to Study Diversity

in Soil Metagenome and it’s Significance for Sustainable Soil Management”, In "Soil

Microbiology & Biotechnology" M. Miransari. Ed. Houstan, Texas: Studium Press

LLC, 2013, Chapter 1.

7. S. Krishna Sundari and N. Mishra. “Contribution of Plant Growth Promoting

Microorganisms for sustainable agricultural and forestry management practice”. In

Soil Microbiology and Biotechnology Ed. M. Miransari. Houstan, Texas: Studium

Press LLC, 2013, Chapter 12.

8. S. Krishna Sundari and K. E. Nandini. “A systematic study of advances in Plant-

stress biotechnology, processes involved and approaches for countering stress”.

Biotechnological Techniques of Stress Tolerance in Plants. Studium Press LLC,

Houstan, Texas 2013, Chapter 4.

9. Rana R., Punyani K., Gupta V.K., Gaur S. Biotechnological Attributes of Phytases:

An Overview In: Applications of Microbial Genes in Enzyme Technology (Eds. V.K.

Gupta, M. G. Tuohy, G.D. Sharma, and S. Gaur) Nova Science Publishers,

USA,.2013.

10. Indira P. Sarethy and Kailash Paliwal (2013) “Evaluating phytoremediation using in

vitro plant cultures” in Modern Biotechnology and its Applications, Part-I, (ed. K.

Behera) New India Publication Agency, India, 2013, Chapter 3, pp 57-87.

11. S Krishna Sundari. (2012). Organic pollutants in agricultural soils, risks involved

and options for remediation. In “Environmental Biotechnology-Recent

Perspectives: Application and New Horizons of Environmental Biotechnology”. Eds.

N. Joshi, K.C. Sharma, M. Sharma. Lambert academic Publishing, Gmbh & Co., KG.,

2012. pp. 194-232, . ISBN: 978-3-8484-2515-0

12. Gaur, S. and V K Gupta., “Biotechnological Perspective of Bacterial Proteases: An

Overview”in Biotechnology of Microbial Enzymes, Nova Science Publishers, USA

2012, pp 69-79.

13. Kushagr P, Shuchi A, Vibha R. “Metagenomics: A new tool to explore the

uncultured microbes in their natural habitats” in Recent Advances in

Environmental Biotechnology, Lambert Academic Publishing, Germany. 2011

14. Vibha, R., Indira, P.S., Diksha, G., Karthikeya, T., Mayank, C., and Neha, S. (2011)

‘Defense signaling pathways in Arabidopsis thaliana: a model host plant to study

plant pathogen interactions”- ‘Advancement of Biotechnology’, International Book

Distributing Co., Lucknow, India

15. Gaur, S., Ahmad, N. and Maheshwari, S., “Impact of fungal phytases in

biotechnology: present and future perspectives. In: Fungal Biochemistry and

Biotechnology, (Eds. Gupta, V.K., Tuohy, M.G. and Gaur,R.K.) Lambert Academic

Publishing, Germany. (ISBN No. 978-3-8433-5800-2), pp 20-34, 2010.

16. Vandana Gupta and Sanjay Gupta, Diversity of Microbial World: General Microbiology (chapter in e-book for first year undergraduate students), Council of Scientific and Industrial Research (CSIR), Government of India, 2008.

Annexure BT-3

PLANT & MICROBIAL BIOTECHNOLOGY List of Doctoral students

Ongoing

S.No

Year of Registration

Name Title Supervisor

1 2009 Sujata Basu

Preventive effects of Salacia extract in oxidatively stressed condition

Rachana

2 2009 Nivedita Mishra

Developing microbial consortia with abilities for plant growth promotion and remediation of residual pesticides

Krishna Sunadri

3 2009 Mamta Pant

To study the preventive role of Adhatoda vasica in oxidatively stressed condition

Rachana

4 2011 Anuradha A study on Value added products from Aroid (Amorphophallus paeoniifolius)

Neeraj Wadhwa Susinjan Bhattacharya

5 2011 Parul Sharma

Biopolymeric membrane for animal cell culture Ashwani Mathur Pros. S Chand

6 2012 Sonam Shaheen

Mass Production of PGPR for making microbial consortium and testing their ability to remediate organophoshphate pesticides

Krishna Sundari

7 2012 Ibeyaima Bioprospection of actinomycetes from Indian Desert for antimicrobial activity and other natural products of industrial importance

Indira P Sarethy Prof. S. Sharma Prof. R. Lal

8 2014 Swarna Shikha

Phytoremediation Pammi Gauba

9 2014 Samiya Khan

Development of a biocatalyst for refining diesel Nidhi Gupta

10 2014 Nidhi Srivastava

Bioprospection of niche habitat(s) for anti-microbial products

Indira P Sarethy

11 2014 Pragya Bhardwaj

Studies on production of therapeutically important saponins using in-vitro culture of Bacopa monnieri

Ashwani Mathur Chakresh K Jain

12 2014 Pratibha Yadav

Remediation of organophosphate pesticides using PGPM

Krishna Sundari

PLANT & MICROBIAL BIOTECHNOLOGY List of Doctoral students

Completed

S.No. Name Title Supervisor Degree

Awarded

1 Smriti

Gaur

Studies of Proteases from Biological

Sources

Neeraj Wadhwa

2010

2 Sarita

Agrahari

Production of enzymes and degradation

of feathers by soil microbes

Neeraj Wadhwa

2011

3 N. Kumara

Swamy

Paper mill effluent: Decolorisation and

detoxification studies using chemical and

microbial methods

Indira P Sarethy 2012

4 Gajendra

Bahadur

Singh

Microbial screening and expression of

gene involved in carbazole degradation

Nidhi Gupta 2013

Annexure BT-4

PLANT & MICROBIAL BIOTECHNOLOGY Dual degree B.Tech/M.Tech projects

Completed Projects

S.No. Enrl No. Name Project Title Faculty

1 20002 Sharadwata Pan

Expression of Metal binding Proteins/peptides in bacterial cells (E.coli)

Dr. Susinjan

2 20008 Dushyant Pandey

Pegylation, a novel concept in protein modification

Dr. Indira

3 20098 Varun Roy Effect of fungal protease on levels of proteases, gliadin, glutenin in developing wheat embyros

Dr. Neeraj

4 20045 Raghuraj Singh Dangi

Cloning and expression of mosquito larvicidal cry 4a protein of Bacillus thuringiensis subspecies israelensis in E.coli HB101.

Dr. Krishna

5 20053 Sonal Nangalia

Antibacterial properties of allicin from garli+c3c extract: a potential for clinical trials

Dr. Reema

6 20070 Prashant Kishore

Bioproduct characterization and analytical method validation

Dr. Indira

7 20084 Shashank Shekhar Singh

Media scouting for optimization of growth of adherent cell line

Dr. Indira

8 20069 Sunil Kumar

Production of cellulase enzyme from Agaricus bisporus by solid state fermentation

Dr. Krishna

9 20081 Shree Prakash

Expression of spermidine-binding protein PotD in Escherichia coli

Dr. Susinjan

10 6101060 Anjali Sharma

Development and characterization of topical microemulsion system for Camellia sinensis extract

Dr. Reema

11 6501805 Bharti Sharma

Investigating the effect of plant metabolites on yeast cells subjected to oxidative stress

Dr. Krishna

12 6501815 Nikhil Kathuria

Potential of Picrorhiza kurroa extract in preventing "Tobacco smoke" induced apoptosis in THP-1 cell line

Dr. Rachna

13 6501829 Swati Chabbra

Investigating the effect of fungal metabolites on yeast cells subjected to oxidative stress

Dr. Krishna

14 6501825 V. Divya Sai

Developing mutants with increased PHA production on alternate substrates

Dr. Krishna

15 6501826 Yashi Saxena

Amylase production and characterization from alkaliphilic isolates

Dr. Indira

16 6501827 Varun Kohli

Substrate and process optimization for maximising PHA production on alternate substrates

Dr. Krishna

17 7501821 Prakhar Sachdeo

Generation of metal binding E.coli through surface display of engineered outer membrane protein

Dr. Susinjan

18 7501823 Atul kumar

Dehydration and image analysis of Vitis vinifera

Dr. Neeraj

19 7501824 Aarushi Kashyap

In vitro propagation of the medicinal plant Solanum nigrum in liquid media and evaluation of its antioxidant property

Dr. Indira

20 7501825 Purva Chopra

Production and Extraction of Biosurfactant from Streptomyces sp. PN-18

Dr. Indira

21 7501828 Nitin Goel An Investigation of the possible preventive role of apocynin on smoke induced cell death, in vitro

Dr. Rachna

22 7501829 Harsha Rohatgi

Production of Resistant Starch from Plant Sources

Dr. Neeraj

23 7501830 Vartika Mahajan

Isolation, Purification and Characterization of Protease from Vegetable Waste

Dr. Neeraj

24 7501834 Ayushi Jain

An Investigation of possible preventive role of Tinospora cordifolia on Smoke induced cell death: Yeast Model

Dr. Rachna

25 7501835 Aishvarya Bioprospecting For Actinomycetes In Arid Desert

Dr. Indira

26 7501806 Uday Bahal

In-vitro propagation of the medicinal plant Bacopa monnieri in liquid culture and effect on saponin production

Dr. Indira

27 7501816 Jai Surabhi Verma

Production Of Proteolytic Enzyme Keratinase By Free And Immobilized Cells Of Bacillus Species

Dr. Neeraj

28 7501819 Gaurav Kumar

Wheat gluten and puroindoline as edible food coating

Dr. Neeraj

29 07501811 Deepika Antiapoptotic activity of bioactive compounds from selected fungi

Dr. Krishna

30 7501832 Sanchit Srivastava

Decomposition of Lycopersicon esculentum (tomato) and Citrus limonium (lemon) leaf litters under Laboratory conditions

Dr. Neeraj

31 7501807 Ishan Wadi

Studying the interactions of active ingredients from salacia reticulata with Aldose reductase: in silico approach

Dr. Rachna

32 8101013 Yashi Bhatnagar

Degradation of carbazole by entrapped and encapsulated Pseudomas sp.

Dr. Nidhi

33 8512003 Deepak Kumar Bharti

Bacteriological and physicochemical quality of drinking water in West Delhi, India.

Dr. Smriti

34 7501830 Vartika Mahajan

Isolation, Purification and Characterization of Protease from Vegetable Waste

Dr. Neeraj

36 9101059 Mansi Sehgal

Profiling of Bacopa monnieri , from different geographical habitat, for phytochemicals of therapeutic importance

Dr. Ashwani

37 9501810 Akansha Sachdeva

Development of non-dairy probiotic

38 9501803 Rohan Chhabra

Fungal chitosan: carrier material for animal cell culturing

39 9101064 Mitika Gupta

Characterization of selected actinomycete isolates from dune ecosystem

Dr. Indira

40 9501801 Vandana Yadav

Characterization of selected actinomycete isolates from arid desert

41 9501806 Gaurav Shukla

Properties of edible coatings from native and modified aroid starches

Dr. Neeraj

42 9501822 Mahima Malik

Effect of gluten coating enriched with bioactive compound to improve the quality of fresh cut fruits and vegetables 43 9501807 Apoorva

Gaur Production and purification of tannase from SSF, merits of co-culture for increased yield

Dr. Krishna

44 9501827 Pratima Mishra

Bioprocess parameter optimization for in vitro propagation of medicinal plants

Dr. Garima

45 9501828 Ravish Rana

Screening and isolation of vanillin producing microorganisms

46 9501824 Anukriti Verma

Evaluation of probiotic characteristics of bacteria isolated from fermented foods.

Dr. Smriti

47 9501816 Abhishek Rathore

Removal of azo dye by bacterial isolate

48 10101020 Niyanta Bhatia

Characterization of endophytic microorganisms for bioactivity

Dr. Indira

49 10501830 Taru Gupta

Antimicrobial activity of an endophytic streptomyces from Phyllanthus niruri

Dr. Indira

50 10501818 Kirti Chauhan

Screening of Indian medicinal herbs for cell death

Dr Rachana

51 10501823 Akanksha Mohindra

Biodegradation of phenols Dr Neeraj

52 10501831 Harleen Kaur

Biodegradation of Crude oil hydrocarbons Dr Nidhi

53 10101014 Aalapti Singh

Application of phytoremediation technology in remediation

Dr.Pammi

54 10101023 Prachi Fungal chitosan and its membranes: preparation, characterization and application in enzyme immobilization and cell proliferation

Dr. Ashwani

55 10501817 Sukriti A study exploring effect of organophosphate pesticides on oxidative stress metabolism in saccharomyces yeast

Dr. Krishna

Annexure BT-5

PLANT & MICROBIAL BIOTECHNOLOGY

B.TECH PROJECTS

Completed Projects

S.No. Enrl. No. Name Project Title Faculty 1 20019 Greta Kasliwal Establishment of an optimal

micropropagation technique for mass propagation at Jatropha curcas L

Dr. Neeraj 2 20072 Neha Gupta

3 20099 Jiwateshwar Singh 4 20015 Vipul Jain Designing fuel cell using halophiles

and Hydrogen metabolisers Dr. Krishna 5 20030 Swatantra Kumar

6 20039 Nitesh Raut 7 20002 Sharadwata Pan Tissue culture and micropropagation

studies on the living fossil Cycas sps. for potential commercial application

Dr. Indira 8 20029 Abhishek Neeraj 9 20033 Kumar Shivam

Shrivastava 10 20070 Prashant Kishore 11 20014 Sumanth Alla Cost-effective protocols for rapid

micropropagation of rare succulents

12 20007 Piyush Gupta Study of the antagonistic microorganisms against milk protein and fat degradative microorganism

Dr. Susinjan 13 20042 Anurag Sharma

14 20078 Amit Pramod Aggarwal

15 20109 Kapil Mishra 16 30025 Abhishek Tyagi Isolation, identification and

characterization of Streptomyces isolates from India for potential production of anti-cancer metabolites

Dr. Indira

17 30040 Nikhil Shanker

18 30036 Nitin Vikram

19 30003 Anmol Grover Isolation of eDNA and biodiversity study of metal metabolizing microbes

Dr. Krishna

20 30010 Harish Sharma

21 30084 Hemant Jethwani Bioplastic Production 22 30081 Mansi Varma 23 40003 Neha Panjiar Isolation, identification and

characterization of Streptomyces isolates having potential petrol and diesel degrading properties.

Dr. Indira 24 40005 Praveshika

Katiyar

25 40018 Tishya Tripathi Isolation of a protease inhibitor from leaves of neem (Azadirachta indica)

Dr. Neeraj

26 40004 Vritika M Gaur 27 5101022 Akshay Banga Screening of dibenzothiophene

desulfurizing microbes from soil and quantification of dibenzothiophene utilization

Dr. Nidhi 28 5101026 Anuj Garg 29 5101028 Yashika Khanna 30 5101080 Pratyush Banga 31 5101021 Shabnam Sourav Novel Enzymes from alkaliphilic Dr. Sanjay 32 5101024 Prerna Kumari

33 5101040 Neha Arora bacteria Dr. Indira 34 5101100 Suyashi 35 5101007 Shubham Tyagi Protease extraction from senesced

leaves and its potential commercial Dr. Neeraj

36 5101018 Pranjal Gupta 37 5101019 Parul Gupta 38 5101032 Nishika Malhotra Study exploring bioremediating

abilities of plant growth promoting microorganisms

Dr. Krishna 39 5101036 Divyanshu Jain 40 5101043 Kumar

Siddhartho Talukdar

41 5101044 Meha Saxena 42 601010 Neha Ansal Protease extraction from senesced

leaves and its application in food industry

Dr. Neeraj

43 601024 Swati Aggarwal

44 601830 Apoorva Gupta 45 601014 Padmini

Munukutla Use of Glutenin coating in the storage of apple slices 46 6101032 Akanksha Gupta

47 6101046 Nisha Agarwal 48 6101031 Arushi Goel 49 601025 Tushar Agarwal Phytochemical Screening.

Purification and Characterization of cellulase from germinating garlic 50 601038 Esha Batra

51 601011 Neha Juneja 52 601048 Pavni Kaushiva

53 601055 Sumit Lamba Isolation and characterization of new proteases from plant seeds

Dr. Neeraj 54 601061 Vipul Jain

55 6101026 Varun Gupta Anti-oxidant and anti apoptotic activity Picrorhiza kurroa : LPS induced model”

Dr. Rachna 56 6501815 Nikhil Kathuria

57 6501828 Vaibhav Gandhi

58 6101015 Prateek Goel Design of portable microbial fuel cell that meets small power requirements

Dr. Krishna 59 6101016 Prateek Roy

Chowdhury 60 6101037 Dhananjai Sinha

61 6101058 Vikram Kapoor

62 6501825 Divya Sai Microbial bioplastic production using oil cakes as alternate substrate 63 6501827 Varun Kohli

64 6101034 Amumeha Shah Developing consortium of plant growth promoting microorganisms for remediation of agricultural soils

65 6101040 Jyoti chauhan 66 6101041 Kanika Sharma

67 6501805 Bharti Sharma Biochemical analyses of anti oxidative compounds in mycelia culture of ectomycorrhizal fungi

68 6501829 Swati Chhabra

69 6101002 Akanksha Srivastava

Screening of dibenzothiophene or carbazole utilizing microbes and

Dr. Nidhi 70 6101003 Ashmita Saigal 71 6101020 Sakshi Aggarwal

72 6101022 Shivani Bisht quantifying the extent of utilization 73 7101001 Sneh Sharma Oxidative stress linked antiapoptotic

activity of secondary metabolities (polysacharrides) and enzyme (SOD) from fungi

Dr. Krishna 74 7101036 Aakriti Goel 75 7101023 Ridhie Bajaj 76 7501811 Deepika 77 7101028 Anubhuti Bansal To study the ability of plant growth

promoting microorganisms to remediate carbofuran and chromium

78 7101034 Megha Sarawgi

79 7101058 Neha Goswamy

80 7101064 Amit Chawla Design and optimization of a portable microbial fuel cell that meets small power requirements

81 7101012 Anant Aggarwal

82 7501806 Uday Bahal Microrhizome production from black turmeric and its characterization

Dr. Indira 83 7501824 Aarushi Kashyap 84 7501831 Nidhi Sejwal 85 7501835 Aishvarya Molecular characterization of

Streptomyces isolates 86 7501801 Megha Garg 87 7501825 Purva Chopra 88 7501812 Vidhi 89 7501819 Gaurav kumar Optimization studies for keratinase

production Dr. Neeraj

90 7501823 Atul kumar 91 7501832 Sanchit Srivastava

92 7101047 Mayank Singh 93 7501816 Jai Surabhi Verma Identification of prebiotics from

plants 94 7501829 Harsha Rohatgi 95 7501830 Vartika Mahajan 96 7101006 Akanksha Gulia Selection of native isolates with

optimal tannase activity on alternative substrates

Dr. Rachna 97 7101035 Swaran Nandini

98 7101005 Parul Mehrotra

99 7501807 Ishan Wadi Potential role of Apocynin in preventing Apoptosis: yeast model

Dr. Rachna 100 7501828 Nitin Goel

101 7501834 Ayushi Jain 102 7101063 Akash Mathew Screening, Isolation and

identification of microbes producing phytate degrading enzymes (phytase).

Dr. Smriti 103 7101056 Nidhi Magoo 104 7101057 Srishti Rawal 105 7101060 Gaurav Shah Expression of metal binding peptides

in Escherichia coli Dr. Susinjan

106 7501821 Prakhar sachdeo 107 7101041 Shainkee

Chauhan 108 8101001 Manal Shakeel Developing mass production

strategies for PGPMs Dr. Krishna

109 8101011 Shivesh Ghura 110 8101035 Anushka Jain Exploring the plant growth

promoting and bioremediating activities of PGP bacteria

111 8101057 Ambika Ramrakhiani

112 8101031

8101025 8101044 8101013

8101031 8101025 8101044

Poorva Mehndiratta

Carbazole degradation carbazole degradation by immobilized cells

Dr. Nidhi

113 8101025 Arushi Jain

114 8101044 Tanya Pahwa 115 8101013 Yashi Bhatnagar 116 8501801 Sakshi Goel Studying the biocontrol properties

and benzopyrene degrading abilities of Trichoderma

Dr. Krishna Dr Sujata

117 8101034 Akshara Rawat Optimization Studies for phytase Production

Dr.Smriti 118 8101055 Rashi Bhutani 119 8101038 Manmeet Singh

Dayal Production of bacterial cellulose from Acetobacter sp

Dr. Ashwani

120 8101045 Vibhor Jain 121 8101047 Rohan Seth Purification and properties of

alkaline amylase from alkaliphilic bacteria

Dr. Indira 122 8101053 Harsh Sharma

123 8501803 Sangey Dorji 124 8101058 Harsh Tandon Optimization of phytase production

by response surface methodology Dr. Pammi

125 9101011 Chandni Verma Cytoprotective role of Andrographis paniculata on Nicotine induced toxicity in yeast cells

Dr. Rachna 126 9101057 Shreya Ahuja 127 9501813 Nidhi Dogra 128 9101039 Gautam Kapoor Interaction of 11 hydroxy

mustakone from Tinospora with biological receptors

Dr. Rachna

129 9501804 Preeti Mishra Production of phytate degrading enzyme by soil isolate

Dr.Smriti

130 9501816 Abhishek Rathore 131 9101035 Sakshi Monga

132 9101042 Devyani Shukla 133 9101019 Shreya Mahajan Exploring the ability of native

microbes to produce tannase from alternative sources

Dr. Krishna 134 9501807 Apoorva Gaur

135 9101052 Iram Hasan Study of phosphate solubilising bacteria in plant growth promotion

Dr. Krishna 136 9101034 Kencho Wangdi In vitro propagation of Bacopa

monnieri in liquid culture Dr. Indira

137 9101051 Priya Gaur Diversity of Actinomycetes in desert ecosystem

Dr. Indira 138 9512001 Swati Sharma 139 9101009 Abhishek Sahu Phytoremediation for heavy metals Dr. Pammi

140 9101055 Deepika 141 9501808 Ashutosh K

Mishra 142 9512005 Akanksha Singh

143 9101001 Ashutosh Sharma Screening and identification of microorganisms degrading 7-ketocholesterol

Dr. Nidhi

144 9101018 Deepika Singhal 145 9101036 Soni Dayal 146 9101064 Mitika Gupta 147 9101013 Shikha Arora Process Parameters Optimization for

Cellulose production from Acetobacter sp.

Dr. Ashwani 148 9501827 Pratima Mishra

149 9501803 Rohan Chhabra Bioprocess Parameters Optimization

150 9501810 Akansha Sachdeva

for Chitosan production from soil fungal isolates

151 9101020 Chhavi Kumar Process parameter optimization for laccase production in solid state fermentation

Dr. Garima

152 9101065 Anushree Jaiswal 153 9101066 Richa Nigam 154 9501809 Medhavi Vishnoi

155 9512008 Nidhija Roy Evaluation of antimicrobial potential of selected Indian medicinal plants 156 9101038 Kshitiz Chandrika

Srivastava Therapeutic potential of Amorphophallus paeoniifolius

Dr. Neeraj

157 9101047 Anwesa Banerjee 158 9501806 Gaurav Shukla Purification and application of

enzymes from Amorphophallus paeoniifolius

159 9512002 Parag Gupta

160 10101055 Ayushi Jain Bioprocess parameters optimization for in-vitro propagation of Mentha sp

Dr. Ashwani 161 10101065 Saloni Rao 162 10101066 Shweta Agrawal 163 10101098 Ritika Jain 164 10101035 Aarushi Dua Production and characterization of

microbial cellulose Dr. Garima

165 10101096 Anushuya Raj Das 166 10501801 Samiksha Kukal 167 10501831 Harleen Kaur 168 10101020 Niyanta Bhatia Study of endophytic bacteria from

selected medicinal and non-medicinal plants.

Dr. Indira

169 10101031 Deeksha Gupta 170 10501802 Bhavika Sharma 171 10501830 Taru Gupta 172 10101045 Nikhita Agarwal Investigating the role of PGPMsin

assisting plant growth under abiotic conditions

Dr. krishna 173 10101075 Kriti Shukla 174 10101081 Srishti Dangayach 175 10501817 Sukriti 176 10101006 Abhisarika

Patnaik Screening of plant poly phenol oxidase for its decolourisation and detoxification potentia

Dr. Neeraj 177 10101016 Nabeel Zaman 178 10101037 Aditi Bhatnagar 179 10101039 Garima Naswa 180 10101004 Sonal Gahlawat Phytoremediation for PPCPs Dr. Pammi 181 10101017 Shubha

Valsangkar 182 10101059 Manvi Makhijani 183 10501818 Kirti Chauhan 184 10101056 Jasveen Kaur

Chadha Nicotine induced cell death in yeast cells

Dr. Rachna 185 10101062 Neha Choudhary 186 10101001 Sukrati Srivastava 187 10101092 Kritika Sharma

188 10101050 Shruti Garg Escherichia coli surface display using outer membrane protein C

Dr. Susinjan 189 10501809 Shilpa Mishra 190 10501810 Vandana Sharma 191 10501828 Kopal Jalan 192 10101043 Monika Rani Optimization of culture conditions

for the production of phytase from Dr. Smriti

193 10101063 Sanchita Agarwal 194 10101078 Sakshi Madan

195 10501823 Akanksha Mohindra

soil bacteria 196 10101090 Pranav Sood Isolation of microorganism

degrading Napthalene

Dr. Nidhi

197 10101072 Farah Siddiqui 198 10101074 Ishan Shekhar 192 11501822 Suramya Asthana Dr. Neeraj 193 11501817 Mohit Vadehra 194 11101027 Meenakshi Sarpal 195 11101013 Astha Upadhyay Bacterial cloning of CspD Dr Susinjan 196 11101049 Shruti Thakur

197 11101055 Stuti Mahajan 198 11101017 Divya Tiwari Effect of Biosurfactant on carbazole

degradation Dr Nidhi

199 11101025 Kuldeep Nigam 200 11101038 Pranjul Rai

201 11101019 Farhein Akmal Cloning of gene involved in petroleum refining

Dr Nidhi 202 11501807 Deepshikha

Chakraborty 203 11501818 Mridul Trehan 204 11501805 Avantika Rawat Role of Tinospora cordifoila on yeast

cell death induced by carbon soot Dr Rachana

205 11501813 Jahnavi Sharma 206 11101054 Srishti Singh 207 11101014 Bharti Sharma Diversity of endophytes in the

aquatic plant Nelumbo nucifera Dr Indira

208 11101058 Tanuja Ijarwal 209 11101018 Faiza Khan 210 11101006 Anchal Sachdeva 211 11101033 Nishtha Grover Therapeutic potential of Probiotic

Seabuckthorne Juice Dr Ashwani

212 11101001 Aanchal Budhraj 213 11101031 Nikita Gupta 214 11101053 Srishti Kotiyal Biopesticides from plant sources Dr Krishna 215 11101037 Poorva Singhai 216 11101032 Nilanshu Gupta 217 11101047 Shefali Goyal Phytoremediation of Pollutants in

soil Dr Pammi

218 11101008 Anjali Verma 219 11101009 Ankita Prakash 220 11501816 Meghna

Srivastava Bacterial expression of metal binding peptides

Dr Susinjan 221 11501812 Ira Thapa 222 11101060 Vrinda

Kulshreshtha 223 11101041 Raveesha Malhotra

Production of exopolysaccharide from lactic acid bacteria

Dr. Smriti 224 11101005 Ananya Singh 225 11101021 Gauri Mittal 226 11101050 Siddhant Sharma

Annexure BT-6

PLANT & MICROBIAL BIOTECHNOLOGY Relevant Courses conducted

Microbiology (10B11BT313)

History and scope of microbiology; Broad classification and taxonomy of microorganisms;

Growth and physiology; Methods of microbial enumeration; Microbial control; Microbial

metabolism, photosynthesis, fermentation, anaerobic respiration; Pathogenic

microorganisms (bacteria, fungi, protozoa, and viruses, etc), host-pathogen interactions;

Microbial genetics; Extremophiles; Microbes in industry.

Cell Culture Technology (10B11BT412)

Cell culture materials and tools, growth conditions and other requirements for

establishment and maintenance of plant and animal cells, cell lines and tissues; in

vitro conservation, protoplast, triploid & haploid culture, micropropagation; Animal cell

cultivation: primary culture, growth kinetics, biology and characterization of cultured cells,

Large scale production of biologicals in plant and animal cells.

Genetic Engineering (10B11BT513)

Concepts in Genetic Engineering, Enzymes in Genetic Engineering, Cloning Vehicles, BAC /

YAC vectors, Construction & screening of genomic libraries, gene cloning strategies, DNA

sequencing & mutagenesis, Cloning & expression of transgenes in Prokaryotic & Eukaryotic

systems, PCR technologies, gene transfer in plant and animals, molecular markers,

Applications and impact of rDNA technology, Ethical issues and biosafety regulation.

Food and Agricultural Biotechnology (10B11BT612)

Food chemistry, microbiology of fermented food products, food spoilage and food borne

diseases, Food processing and preservation, Current status of Indian processed food

industry, Use of enzymes in food industry, nutraceuticals and functional foods, Single Cell

Proteins, Probiotics, Biotechnological approaches in production of therapeutics & industrial

products in animals and plants, Production of value added products (biofuel, bioplastics, etc)

from agricultural waste, traditional crop improvement vs biotechnological interventions.

Fermentation Technology and Downstream Processing (10B11BT614)

Introduction to fermentation processes, Isolation, preservation and improvement of

industrially important microorganisms, Media optimization, Bioprocess Considerations for

animal & plant cell cultures, Downstream Processing : Filtration, centrifugation, Separation

of insoluble products – Cell disruption : Physical methods, Chemical methods, Separation of

soluble products- liquid-liquid extraction: solvent recovery, two phase aqueous extraction,

Chromatography, Process design of Industrial Bio-products : Anaerobic bioprocesses –

Ethanol and lactic acid production, Aerobic bioprocesses – Citric acid and penicillin

production

Enzymes in Food Processing (10B1NBT735)

Principles of enzyme assay and kinetic studies, enzyme units, Carbohydrate Hydrolyzing

Enzymes– amylases, cellulase, Hemicellulases, Isomerase, Pectin degradation Fat

hydrolyzing enzymes Lipases, Phospholipases Application in Beverage, Juice and Wine

making Industry, Enzymes in Dairy Industry, cheese making and ripening aroma and flavor

production, cold sterilization, Enzymes in product modification. Debittering, Hydrolysis of

Soy protein, fish protein, Milk protein, collagen, Blood protein Tailoring enzyme structure

and function Alteration of technical properties, Increasing yields, Raw matter utilization,

Improving preservation, flavors.

Molecular Ecology (11B1NBT831)

Molecular genetics in ecology, Understanding molecular markers, modes of inheritance, co-

dominant and dominant markers, Genetic analysis of single population, Genetic analysis of

multiple population, Molecular markers in phylogeography, molecular clocks, distribution of

genetic lineages, Molecular approaches to behavioral ecology, Applications of molecular

ecology in wildlife forensics, agriculture and fishing.

Process Engineering (10B11BT512)

Microbial process development: Introduction to Upstream and Downstream processes,

batch, Fed Batch and Continuous culture, Cell growth kinetics; Bioreactor systems including

utilities: Types of bioreactor and their applications, cardinal rules of bioreactor design,

utilities of bioreactors; Fluid flow and mixing – Rheological properties – Newtonian and Non-

Netonian fluids, Flow behavior, mixing, power consumption and shear properties of

rushton turbine, helical, anchor, bubble column, external loop, airlift etc; Heat transfer –

different modes of heat transfer, Mass transfer in microbial processes: Mass transfer

correlation for air solubility; Sterilization : Thermal death of micro-organisms, Batch and

continuous sterilization of media, Design of sterilization equipment; Bioreactor analysis –

Ideal and non-ideal reactor: immobilized enzyme and cell reactors, multiphase bioreactors,

Operational measurements and control in fermentation: Bioreactor sensor characteristics;

Case studies related applications in various biotech and biopharma industries.

Bioremediation (10M11BT212)

Environment pollution vis-a-vis bioremediation, Nature and fate of environmental

pollutants, Methods and strategies of bio-remediation (ex situ & in situ), Biochemical

pathways of degradation, monitoring of bioremediation, use of microorganisms in oil spill

degradation, oil refining and oil recovery, Remediation of gaseous effluents, Aerobic and

anaerobic methods for sewage treatment, Metal bioremediation, Phytoremediation, GMO’s

for bio-remediation.

Industrial Bioprocessing (12M1NBT431)

Bioprocess engineering principles for non ideal reactors including submerged and solid state

fermentation, growth kinetics for solid state fermentation, Design of bioreactor and scale-

up designs; Downstream processing techniques: concentration, purification, polishing.

Purification of products from mammalian, plant and algal fermentation; Animal Cell

fermentation: Methods of studying cell growth kinetics in bioreactor, Bioreactor design for

anchorage dependent and suspension cell lines, 2D and 3D cell culture; Plant Cell

fermentation: method of studying growth kinetics of plant cells in suspension and callus.;

Algal fermentation: Photosynthetic and chemosynthetic algae of commercial importance,

bioprocess conditions and bioreactor design for algal fermentation, type of metabolites

produced using algal fermentation; Recent development in bioreactor design: engineering

principles of Wave bioreactor, disposable bioreactor, bioreactor for 3D tissue culture,

bioreactor for hairy root culture.

IPR and Bioethics (10B1NBT833)

Different forms of Intellectual Property Rights, their Relevance to Biotechnology Industry

& Academia; Overview of International conventions & Trade agreements: WTO, TRIPS,

WIPO, Implication for developing countries; Process involved in Patenting, Patent Search;

IPR in Agriculture, Farmer’s Rights, UPOV & Traditional Knowledge; Need of bioethics,

Bioethics & GMO’s: Issues of Genetically modified foods; Bioethics in Medicine gene

therapy, Organ transplantation, ethics in patient care; Bioethics & Cloning: Human

cloning, Stem cell research, Use of animals in research, human volunteers for Clinical

trials; Ethics in Profession

Metagenomics (12M1NBT331)

Molecular Diversity and Metagenomics: Concept of e-DNA (environmental DNA), and

introduction to Metagenomics, Diversity of Microbes in different environments;

Conventional methods to study diversity, Understanding the ecosystem level functions of

Microbial Communities; Human microbiome projects by NIH, India and EU; Significance of

Bioinformatics in understanding and analysis of Genomic Data, Databases and Software

available for analysis of Metagenomic Data; Metagenomics & Bioprospecting, Applications

in Medicine, Cancer metagenome; applications in Environmental Biotechnology, Acid Mine

Drainage project, Sargasso Sea Metagenomic Metagenomics & Applications in Agriculture,

The Soil Resistome project

Bioprocess and Industrial Biotechnology (14M11BT211)

Basic kinetic models of cell growth kinetics, media and air sterilization, Cell death kinetics,

metabolic quotient, O2 transfer and uptake, mass transfer and heat transfer, liquid rheology,

Batch / fed batch and continuous cultivation, yield and productivity, Types of bioreactor,

Ideal and non-ldeal reactors, Optimization of bioprocess operation (OVAT and statistical

design), Operative measurement and control of fermentation, Bioreactor scale-up criteria,

Cell growth kinetics of bacteria and fungi in non-ideal reactors: submerged and Solid state

fermentation, types of reactor for solid state fermentation, recombinant bacterial cell

stability and growth kinetics, Animal cell fermentation (Animal cell metabolism: basic

understanding of substrate and byproduct stoichiometry, Growth characteristics and

kinetics, micro-carrier attached growth kinetics, biomaterial properties for anchorage

dependent cell lines, cell culture in perfusion and hollow fiber reactor, 2D and 3D cell

culturing, bioreactor design considerations), plant cell fermentation (Importance of plant

cell cultivation, Plant cell / hairy root cultivation, callus and shoot propagation, kinetics of

cell growth and product formation, plant cell / hairy root reactors – types of rectors,

comparison of reactor performance, immobilized plant cell reactors), Algal derived

metabolites, methods of studying growth kinetics of chemotropic and phototropic algae,

types of bioreactors: lab scale and large scale photo-bioreactor / pond reactors, Isolation,

preservation and propagation of microbial cultures, Process technology for production

of Organic acids, Amino acids, alcohols, antibiotics, Vitamins, nucleotides, steroids &

flavours, Production of enzymes: protease, cellulose, amylase, lipase; Enzyme inhibitors:

inhibitors of cholesterol synthesis; biopesticides; biofertilizers; biopreservatives;

biopolymers; single cell proteins; monoclonal antibodies; phytochemicals of commercial /

therapeutic importance : flavanoids, saponins.

Nutraceuticals (14M1NBT235)

Historical perspective, Classification, scope & future prospects. Bioactive Carbohydrates

Bioactive Lipids, Bioactive Peptides, Polyphenols, Nutraceuticals of plant origin,

Nutraceuticals of animal origin, Microbial and algal nutraceuticals, Nutraceuticlas and

diseases, Product development and clinical trials, Nutraceutical Industry and Market

information.

Microbial Technology (14M1NBT238)

Principles of Microbial Biotechnology and industrially important microbes; Food & Microbial

biotechnology; Microbes in Medical biotechnology; Microbes and Environmental

Biotechnology; Microbes and clean energy production; Regulatory and ethical issues

involved in all of the above.

Product development in Biotechnology (14M1NBT334)

Production of commercially important primary and secondary metabolites like organic

acids, amino acids, Antibiotics, Vitamins and Steroids, Industrial Enzymes, Biopesticides,

Biofertilizers, Biopreservatives, Biopolymers, Biodiesel, Recombinant proteins having

therapeutic and diagnostic applications, Modified and Artificial Enzymes , Catalytic

antibodies, Bioprocess strategies in Plant Cell and Animal Cell culture, Regulatory

compliance, Analytical product testing , Biomanufacturing and Biobusiness issues.

Plant Diseases & Biotechnology (15B1NBT833)

An Introduction about Global Agriculture productivity and how disease impact crop yield;

Use of chemical pesticides, insecticides and their ill effects; Plant physiology, Genetic basis

of Plant genes, plant signalling pathways (systemic acquired resistance); Principle groups of

plant pathogens and diseases caused, Molecular basis of genetic modification and crop

improvement; RNAi technology in plant disease control; developing genetically modified

plants with improved disease resistance; Biocontrol methods applying antimicrobial

proteins, Plantibodies, PGPR and their role in disease control.