PHYSIOLOGY LAB PRACTICALS

Experiment V: Differentiating Isotonic from Isometric Contraction

A. Isotonic Contraction Materials: 1. Dumbell

B. Isometric Contraction Materials: 1. Hand grip

Experiment VI: Hematology

For all experiments, requires:

1. Cotton balls (with alcohol) 2. Syringes

A. RBC and WBC Count Materials: 1. Hemocytometer

2a. Saline Solution (For RBC)

2b. Or 1% Acetic Acid (For WBC)

3. Microscope

4. Filter Paper (or Cotton)

B. Hemoglobin Determination Materials 1. Sahli-Hellige Hemometer

2. Lancet

3. Cotton balls (with alcohol) 4. Distilled Water C. Bleeding Time and Clotting Time Materials: 1. Lancet 2. Glass Slide (plain)

3. Pin or Matchstick 4. Absorbent Paper (Filter paper) 5. Timer

D. Blood Typing Materials: 1. 2 test tubes (containing saline solution)

2. Glass Slide with 2 concavities

3. Matchsticks

4. Blood typing sera

E. Cross Matching Materials: 1. 2 test tubes (containing saline solution) 2. Glass Slide (plain) 3. Applicator Stick

4. Centrifuge

F. RBC Fragility Test (Osmotic Fragility Test) Materials: 1. Big rack with 12 test tubes containing different concentrations of saline solution

2. 0.5% stock solution 3. Distilled water 4. Dropper

G. Hematocrit Determination Materials: 1a. Wintrobe tube containing anticoagulant

1b. Or microhematocrit pipette, heparinized

2. Centrifuge

Experiment VII: Respiratory Experiments A. Static Lung Volume Materials:

1. Spirometer

B. Recording Respiratory Movements Materials: 1. Pneumograph

2. Kymograph 3. Timer C. Duration of Voluntary Apnea Materials:

1. Pneumograph 2. Kymograph 3. Timer D. Measurement of Peak Expiratory Flow Rate Materials: 1. Peak flow meter

E. Examination of Chest and Lungs Materials: 1. Stethoscope

Experiment IX: Examination of the Heart and Pulses Materials: 1. Stethoscope 2. Timer Experiment X: Arterial Blood Pressure Determination

A. Palpatory and Auscultatory Methods Materials: 1. Stethoscope 2. Sphygmomamometer

B. Cold Pressor Test Materials: 1. Stethoscope 2. Sphygmomamometer 3. Pain Perception Scale

4. Bucket of ice cold water (0-5 degrees Celsius)

Experiment XI: Kidney Function Test Materials: 1. Clean small bottles for urine collection

2. Graduated Cylinder

3. Dip Stick

4. Urinometer

Experiment XII: General Senses

I. Cutaneous Senses A. Punctiform Distribution of Sensory Receptors 1. Large Handkerchief 2. Graphing paper with 10x10 mm square hole 3. Fine bristle/Horse hair 4. Pin head

5. Container with ice cold water 6. 4 colored pens/Pencils

B1. Attributes of Sensation: Contrast Materials: 1. Large Handkerchief 2. Beaker 1: filled with warm water 3. Beaker 2: filled with ice water 4. Beaker 3: filled with tap water

B2. Attributes of Sensation: Summation Materials: 1. Large Handkerchief 2. Basin filled with warm water B3. Attributes of Sensation: Adaptation Materials: 1. Large Handkerchief 2. Basin filled with warm water 3. Piece of cork

4. Thermometer

C. Pressure Sense Materials: 1. Large Handkerchief 2. Small bucket (or jar) filled with water and sand

D. Aristotle’s Experiment Materials: 1. Large Handkerchief 2. Marble or any rounded object E1. Synthetic Sense: Two-Point Discrimination Materials: 1. Large Handkerchief 2. Double pointed Compass

3. Ruler

E2. Synthetic Sense: Stereogenesis Materials: 1. Large Handkerchief 2. 3 Different Common Objects (e.g. coin, pen, key)

E2. Synthetic Sense: Vibration Sense (Pallesthesia) Materials: 1. Tuning fork

2. Timer

II. Muscle and Joint Senses (Proprioception)

A. Static Position Sense Materials: none

B. Kinesthesia Materials: 1. Protractor Experiment XIII: Special Senses: Vision A. Visual Acuity for Far Vision Materials: 1. Snellen chart

2. Eye Occluder/Pinhole

B. Visual Acuity for Near Vision Materials:

1. Jaeger Chart

C. Near Point of Distinct Vision Materials: 1. Jaeger Chart

D. Accomodation Reflex Materials: none

E. Color Blindness Materials: 1. Dvorine or Ishihara Plates

F. Holmgren-Type Color Vision Test

Materials:

1. Holmgren-type color vision test kit

2. Timer

G. Blind Spot Materials: 1. Chin Rest

2. Paper for blind spot test

3. Pencil wrapped with white paper

H. Depth Perception Materials: 1. Depth perception kit

2. Ruler or tape measure

Experiment XIV: Taste. Smell, Hearing and Equilibrium

I. Taste Materials: 1. Piece of gauze 2. Sugar in solution and in crystal form 3. Timer II. Smell A. Method of Testing Materials: 1. Large handkerchief 2. Test substance (coffee, mint, onion, garlic) B. Adaptation of Olfactory End Organ Materials: 1. Cotton (to plug nostrils) 2. Large handkerchief 3. Test substance (tincture of iodine) 4. Timer III. Hearing (Should be done in a quiet room)

A. Threshold of Hearing Materials: 1. Large handkerchief 2. Clock or any object with ticking sound 3. Cotton

4. Meter stick B. Intensity of Sound Materials: 1. Sound meter

C. Localization of Sound Materials: 1. Large handkerchief 2. Two coins/any object with ticking sound D. Weber Test Materials: 1. Tuning forks 2. Cotton balls E. Rinne Test Materials: 1. Tuning forks 2. Timer Experiment IV. Equilibrium

A. Simple test for the Horizontal Canals Materials: 1. Meter stick or cane

Experiment XV: Temperature Regulation Materials: 1. Digital thermometer 2. Reading material 3. Timer 4. Snacks 5. Cotton balls with alcohol Experiment XVI: Reflexes in Man Materials: 1. Cotton wisp - corneal reflex 2. Penlight – pupillary light reflex

3. Tongue depressor - gag reflex

4. Reflex hammer - jaw, knee, ankle and plantar reflexes

Experiment XVII: Motor System Examination Materials: 1. Reflex Hammer

Special Mention to Back Row Notes and Physio Manual for the lacking experiments Prepared by Jian Narag Photos by: Kay Ramos (and internet) Model: Deus Mata

Words of Wisdom from Slave Driver: Pio Savaitnisagon;

… Deus Agrees.

Good luck with the Practicals guys!

ARAL!