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Slide 2 Predictions: Is DNA found in the food we eat? Where exactly do you think DNA is located? Can DNA be extracted? Why is DNA extraction an important skill for a forensic scientist? Slide 3 Slide 4 Every species has their own DNA sequence Scan 13 different regions (loci) when creating a human DNA profile Human DNA Profile=DNA Fingerprint Slide 5 DNA left at a crime scene Exonerate persons who are wrongfully accused of crimes Identify crime and catastrophe victims Establish paternity and other family relationships Slide 6 DNA id is effective if used intelligently. Example: Assume that type O blood is found at crime scene. Type O occurs in about 45% of Americans. If investigators type only for ABO, finding the suspect with type O blood doesnt reveal much. Its usually a chain of biological and physical evidence like hair, footprints that leads to the making of the match. Slide 7 RFLP Restriction Fragment Length Polymorphism PCR Analysis Polymerase Chain Reaction STR Analysis Short tandem repeat technology Mitochondrial DNA Analysis *related to the DNA loci passed on from the mother Y-chromosome analysis *passed on loci from the father Slide 8 Identifying September 11 th victims Migrational Patterns of Human throughout history DNA Banks for Endangered Species Poached Animals Slide 9 Strawberry: large genome due to the fact that it is an octoploid (8 times the amount of genetic info.) Soap: dissolve the phospholipid bilayers of the membrane and organelles (plant cell) Salt: break up the protein chains that bind the nucleic acids Ethanol: is kept cold on ice because the DNA is insoluble making it easy to separate from the beaker. Slide 10 Online is posted the conclusion worksheet. Answer all questions on your own sheet of paper. Attach your answers to the rest of your lab report. Lab report due Wednesday.