Prep 150 LC SystemThe perfect combination of intuitive software and robust instrument performance

PREP 150 LC SYSTEM CONFIGURATIONChoose from an array of Prep 150 LC System options and tailor to your laboratory’s purification needs.

Detection technologies:

■■ 2489 UV/Visible (UV/Vis) Detector

■■ 2998 Photodiode Array (PDA) Detector

Sample injection:

■■ 2707 Autosampler

■■ Prep Injector Module

Solvent delivery systems:

■■ 2545 Binary Gradient Module (BGM) high pressure mixing binary gradient pump*

■■ 2545 Quaternary Gradient Module (QGM) low pressure mixing gradient pump*

Fraction collection:

■■ Waters Fraction Collector III (WFC III)

*System flow rates up to 150 mL/min, capable of mg – g purification and columns up to 50 mm I.D.

A V ERSAT ILE SYST EM TO SUIT YOUR PURIFICAT ION REQUIREMENT S

Developed for dedicated personal purification requirements or quick adoption in a

centralized lab, the Waters® Prep 150 LC System, featuring intuitive ChromScope™

Software, is for scientists requiring robust system performance. Ideal for initial workflow

criteria where rapid separations of complex mixtures are required, this versatile system is

designed to satisfy processing requirements of few to many samples a day with confidence.

The Prep 150 LC System shown with the 2545 BGM, Prep Injector Module, 2998 PDA Detector, and WFC III.

SIMPLIF IED PURIFICAT ION

Purification is made simple with ChromScope Software. The easy-to-navigate intuitive

screens provide real-time, useful system and data information at a quick glance.

■■ Easy icon-driven interface for quick access to instrument parameters or system control.

■■ Straightforward wizards guide the user while creating methods.

■■ Easily navigate with quick tabs to select methods, sample sets, system configurations, system status, or run status with one click.

■■ System messages providing up-to-the-minute status on instrumentation and purification runs.

Waters ChromScope Data Software for the Prep 150 LC Purification System.

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ST RAIGHT FORWARD SCALE-UP

Confidently scale methods from analytical to prep using Waters OBD™ Preparative Columns and the Waters OBD Preparative Column

Calculator. Shown here is an analytical to prep method using XBridge® OBD Preparative Columns. Easily scale up to our more sophisticated

AutoPurification™ System when full automation and higher levels of specificity and purity are required. 1.875

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3.549

4.135

4.706

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1.4 mL/min 0.1% formic acid in water/acetonitrile

95:5 to 30:70 over 5.5 minutes

XBridge BEH C18, 5 µm, 4.6 mm x 100 mm Column

ACQUITY UPLC® H-Class System

24 mL/min 0.1% formic acid in water/acetonitrile

95:5 to 30:70 over 5.5 minutes

XBridge BEH C18, OBD Prep, 5 µm, 19 mm x 100 mm Column

2545 BGM/2489 @ 254 nm/manual injection

Successfully scale analytical to prep methods with Waters OBD Preparative Columns.

The preparative method was acquired by using the Analytical to Prep Gradient Calculator integrated within Waters ChromScope Data Software.

OBD PREPARATIVE COLUMNS FOR HPLC PURIFICATION

Bridging the gap from analytical to prep■■ Increase sample load

■■ Improve resolution

■■ Reduce run time

OBD Preparative Columns.

Productivity comes from predictability. For more than 10 years,

chemists have come to rely on the high purity and recovery

obtained with our OBD Preparative Columns. Extended column

lifetimes and high mass loading capacity lead to

increased productivity. ■■ SunFire™ OBD Preparative Columns – Working at low

pH only, need high loading for bases

■■ XBridge OBD Preparative Columns – Working at high pH only

■■ XSelect® CSH OBD Preparative Columns – Working at both low and high pH, need high loading for bases

■■ Peptide Separation Technology OBD Preparative Columns – Isolation and purification of synthetic peptides

■■ Atlantis® T3 OBD Preparative Columns – For purification of polar compounds

■■ XBridge Amide/Atlantis HILIC/XBridge HILIC OBD Preparative Columns – For purification of polar and highly polar compounds under HILIC separation mode

Select OBD Preparative Columns for speed, robustness,

predictable performance, and ease of scaling from analytical to prep.

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MAXIMIZE SYST EM AUTOMATION

Utilize the stacked injection function to efficiently

separate a large amount of sample with the

simplistic wizard tool, and effortlessly optimize

your purification results.

6.6 mL/min 6:4 methanol/water

SunFire C18, 5 µm, 10 x 100 mm Preparative Column

2545 QGM/2998 @ 254 nm/2707 Autosampler

Stacked injections

Optimize system efficiency and throughput when collecting the same compounds from multiple

sample injections with isocratic methods.

P ROV EN PERFORMANC E AND RELIABILIT Y

Waters high performance preparative solvent delivery systems provide reliable and consistent performance you can depend on. Satisfy your

application requirements with either the high-pressure mixing binary pump or the low-pressure mixing quaternary pump.

0.5 1.0 1.5 2.0 2.5 3.0 3.5 min0-500

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Abs

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30 mL/min 7:3 acetonitrile/water

XBridge BEH C18, OBD Prep, 5 µm, 19 mm x 100 mm Column

2545 BGM/2489 @ 247 nm/manual injection

Overlay of six consecutive injections

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25 mL/min 0.1% formic acid/methanol

10% B to 80% B over 5 minutes

SunFire C18, 5 µm, 19 mm x 100 mm Preparative Guard Cartridge

2545 QGM/2998 @ 254 nm/2707 Autosampler, 500-µL injections

Overlay of six consecutive injections

Multiple injections performed using an isocratic method with the 2545 BGM demonstrates reliable reproducibility required for stringent time-based fraction collection parameters.

Highly reproducible results generated with a gradient method on the 2545 QGM.

Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com

Waters, Waters Quality Parts, T he Science of What’s Possible, ACQUITY, Atlantis, XBridge, and XSelect are registered trademarks of Waters Corporation. AutoPurification, ChromScope, SunFire and OBD are trademarks of Waters Corporation. All other trademarks are property of their respective owners.

©2014 Waters Corporation. Printed in the U.S.A.January 2014 720004637EN KP-IGS

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OPT IMIZE F RAC T ION COLLEC T ION PARAMET ERS AND REPORT RESULT S

ChromScope Software provides key fraction collection parameters, allowing you to select the optimum method to isolate and purify

compounds of interest. Quickly identify fraction locations with the color-coded peak-to-fraction feature.

Robust software featuring collection method development allows you to simulate and optimize your fraction collection parameters without using valuable sample.

Create and customize reports with simplistic drop and drag functionality. Quickly send reports via email or generate a PDF.