presentation do’s and
DESCRIPTION
DON’TS. PRESENTATION DO’S AND. Muscle contains proteins of many sizes. Protein kDa Function titin3000center myosin in sarcomere dystrophin400anchoring to plasma membrane filamin270cross-link filaments into gel myosin heavy chain210slide filaments - PowerPoint PPT PresentationTRANSCRIPT
PRESENTATION DO’S AND
Muscle contains proteins of many sizes
Protein kDa Functiontitin 3000 center myosin in sarcomere dystrophin 400 anchoring to plasma membranefilamin 270 cross-link filaments into gel myosin heavy chain 210 slide filamentsspectrin 265 attach filaments to plasma membranenebulin 107 regulate actin assembly a-actinin 100 bundle filaments gelosin 90 fragment filamentsfimbrin 68 bundle filamentsactin 42 form filaments tropomyosin 35 strengthen filamentsmyosin light chain 27 slide filamentstroponin (T, I, C) 30, 19, 17 mediate regulation of contractionthymosin 5 sequester actin monomers
Phylogenetic TreeSALMONTROUT
Phylogenetic TreeANCHOVIES HERRINGS SARDINES
CARPMINNOW
CATFISH
PIKE
SMELTCODHAKEPOLLOCK FLOUNDER
SOLEHALIBUT
TUNAMACKERELSNAPPER
PERCHWALLEYEBASS
Metazoa
Protostome
ArthropodMollusk
OYSTERCLAMMUSSELSCALLOPOCTOPUSSQUID
CRABLOBSTERSHRIMP
Echinoderm Chordate
Deuterostome
GAR
OstheichthyesChondrichthyes
STURGEON
SHARK
Reptilia Aves MammaliaAmphibiaAgnatha
The stages of mitosis are shown for the onion root tip and the whitefish blastula. The top three slides show the onion root tip. The first is anaphase where the chromosomes are separating. The second is metaphase where the chromosomes line up on metaphase plate. The third is interphase.
The next three pictures are of the whitefish blastula. The first is anaphase where the chromosomes are separating. The second is interphase and the third is telophase.
INTERPHASE
PROPHASE
Methods of transformation
• Electroporation–Electrical shock makes cell
membranes permeable to DNA
• Calcium Chloride/Heat Shock–Chemically-competent cells
uptake after heat shock
Methods of transformation
• Electroporation–Electrical shock makes cell
membranes permeable to DNA
• Calcium Chloride/Heat Shock–Chemically-competent cells
uptake after heat shock
Why perform each transformation step?
Positive charge of Ca+2 ions shields negative charge of DNA phosphates
CH2 N
N H
O
OCH2
OH
O
P O
O
O
O
Ca++
Ca++
Traditional Systematics and Taxonomy
• Classification– Kingdom– Phylum– Class– Order – Family– Genus– Species
• Traditional classification based upon traits:– morphological– behavioral