program of the 14th european meeting on complement and...

Molecular Immunology 56 (2013) 141–150

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Molecular Immunology

journal homepage: www.elsevier .com/locate/molimm

142 Molecular Immunology 56 (2013) 141– 150

14th European Meeting on Complement and HumanDiseases

Jena

August 17 - 21, 2013

www.emchd2013.org

Saturday, August 17, 2013

Leibniz Institute for Natural Product Research and Infection Biology, Beutenbergstr 11a, Jena

8:30-16:30 TEACHING DAY

Lecture Hall, ‘Aula’, Friedrich Schiller University, Fürstengraben 1, JenaAfternoon Registration at the University

‘Hauptgebäude

18:00 Opening Ceremony

Opening of the 14th European Meetingon Complement in Human Disease

Peter F. Zipfel, JenaConference chair EMCHD

Welcome Zvi FishelsonPresident of EuropeanComplement Network

Welcome Address Thorsten Heinzel,Vice Rector for Research,

Friedrich Schiller University,Jena

Plenary LectureComplement and Infection Reinhard Burger

President of Robert KochInstitute Berlin, Germany

from 19:30 Welcome Reception

Molecular Immunology 56 (2013) 141– 150 143

Sunday, August 18, 2013

Volkshaus, Carl-Zeiss-Platz 1, Jena

08:30 Opening of the Scientific SessionChristine Skerka

Session I # Complement Activation and Regulation

08:40 36 Soluble collectin-12 (CL-P1) triggers activation of the alternativecomplement pathwayY.J. Ma, M.-O. Skjoedt, P. Garred, Copenhagen University Hospital,Department of clinical immunology, Copenhagen, Denmark

08:55 70 IgG hexamerization mediates efficient C1q docking and complement-dependent cytotoxicity (CDC)F. Beurskens, R. de Jong, M. Voorhorst, K. Strumane, M.A. Lindorfer,R.P. Taylor, E. Ollmann-Saphire, D. Burton, J. Schuurman, P. Parren,Genmab, Utrecht, Netherlands

09:10 176 C5aR expression in a floxed C5aR GFP reporter gene knockin mouseC. Karsten, K. Bröker, F. Westendorf, Y. Laumonnier, G. Köhl, J. Köhl,Institute for Systemic Inflammation Research, Lübeck, Germany

09:25 157 Assembly and stability of C3 proconvertase and C3 convertase: effectof CFH and C3NeFS. Bettoni, R. Donadelli, S. Ngo, V. Fremeaux-Bacchi, M. Noris, IRCCS -Istituto di Ricerche Farmacologiche Mario Negri, Clinical Research Centerfor Rare Diseases “Aldo e Cele Daccò”, Ranica (BG), Italy

09:40 23 MAP-1, MASP-1 and MASP-3 form heterocomplexes with each otherA. Rosbjerg, L. Munthe-Fog, P. Garred, M.-O. Skjoedt, Rigshospitalet,University of Copenhagen, Department of Clinical Immunology,Copenhagen, Denmark

09:55 122 A novel C3 fragment found in normal plasma and serum: itsimplications for C3 tickoverA. Buchberger, D. Seilly, P. Lachmann, University of Cambridge, VeterinaryMedicine, Cambridge, United Kingdom

10:10 – 10:40 Coffee break

Plenary Lecture

10:40 Multifarious Roles of Sialic Acids in Immunity and HumanEvolution

Ajit Varki , San Diego, CA, USA

Session II # Complement and Infection & Immune Escape of PathogenicMicrobes

11:30 127 Glycerol-3-phosphate dehydrogenase 2 is a novel Candida albicanscomplement inhibitorS. Luo, M. Reza, R. Hoffmann, C. Skerka, A. Verschoor, P.F. Zipfel, Institutfür Medizinische Mikrobiologie, Immunologie und Hygiene, TechnischeUniversität München, München & Leibniz Institute, Jena, Germany

11:45 28 Streptococcus pneumoniae: Endopeptidase O (PepO) a novel C1qbinding complement inhibitorV. Agarwal, K. Riesbeck, M. Fulde, S.Bergmann, A. Blom, Lund University,Malmö, Sweden

12:00 197 CspA from Borrelia burgdorferi inhibits the terminal complementpathwayT. Hallström, C. Siegel, M. Mörgelin, P. Kraiczy , C. Skerka, P.F. Zipfel,


Leibniz Institute for Natural Product Research and Infection Biology, Jena,Germany

12:15 4 The impact of complement evasion on transmission of malariaG. Pradel, N. Simon, E. Lasonder, M. Scheuermayer , A. Kühn, R. Fischer,P.F. Zipfel, C. Skerka , RWTH Aachen University, Institute of MolecularBiotechnology, Aachen, Germany

12:30 159 Defining substrate specificity of gonococcal sialyltransferase and thebasis of complement resistance mediated by gonococcallipooligosaccharide sialylation using CMP-sialic acid derivativesS. Gulati, I. Schoenhofen, D. Whitfield, A. Cox, P. Rice, S. Ram, Universityof Massachusetts Medical School, Division of Infectious Diseases andImmunology, Worcester, United States

12:45 190 The use of a rapid fluorogenic neuraminidase assay to differentiateStreptococcus pneumoniae-associated haemolytic uremic syndrome(HUS) from other forms of HUSÁ. Szilágyi, N. Kiss, K. Kelen, G.S. Reusz, A.J. Szabó, Z. Prohászka,Semmelweis University, IIIrd Department of Medicine, Budapest, Hungary

13:00 – 14:00 Lunch

13:15 – 14:00 Scientific Lunch Symposium“The Swedish Army Knife” of Functional Complement Assays

Supported by EURO DIAGNOSTICA AB13:00 – 14:00 ICS Board Meeting14:00 – 16:00 Postersession I (Posters with even numbers)

Session III # Complement and Innate Immunity

16:00 59 C5a synergizes with IL10 and Toll-like receptors to control theperitoneal B1 cell compartmentK. Bröker, J. Figge, C.M. Karsten, J. Köhl, University-Medical CenterSchleswig-Holstein, Institute for Systemic Inflammation Research, Lübeck,Germany

16:15 181 Regulatory role of C5a in human PMN activity during whole bloodinfection with Candida albicansK. Hünniger, K. Bieber, O. Kurzai, Friedrich-Schiller-Universität Jena,Septomics Research Center, Jena, Germany

16:30 90 Th1 induction requires T cell-derived complement C3a and CD46activationA. Ghannam, C. Kemper, C. Drouet, Université Joseph Fourier, Grenoble,France

16:45 76 Neutrophil components as regulators of the complement systemJ. O‘Flynn, K. Dixon, M.C. Faber Krol, M.R. Daha, C. van Kooten, LeidenUniversity Medical Centre, Nephrology, Leiden, Netherlands

17:00 142 Cholesterol crystals activate the complement system and arephagocytosed in a complement-dependent mannerS. Nymo, E.O. Samstad, N. Niyonzima, M.H. Aune , G. Bergseth, L. Ryan,O.-L. Brekke, E. Latz, J.D. Lambris, T. Espevik, T.E. Mollnes, NordlandHospital, Bodø, Norway

17.15 180 Combined inhibition of C5 and CD14 attenuates organ inflammation inporcine Escerichia coli sepsisK.H. Egge, E.B. Thorgersen, J.K. Lindstad, A.M. Pharo, A.K. Bongoni,R. Rieben, A. Barratt-Due, T.E. Mollnes, University of Oslo/Rikshospitalet,Institute of immunology, Oslo, Norway

17:30 38 Circulating C5a receptor (cC5aR) as a novel biomarker of septic shockin humansS. Denk, D. Rittirsch, H. Barth, J. Sarma, P. Ward, M. Huber-Lang,University Hospital of Ulm, Department of Orthopedic Trauma, Hand-,Plastic-, and Reconstructive Surgery, Ulm, Germany


Monday, August 19, 2013


Session IV # Complement and Disease I

08:30 44 A functional variant in the CFI gene confers a high risk of age-related macular degenerationJ.P.H. van de Ven, S.C. Nilsson, P.L.Tan, G.H.S. Buitendijk, T. Ristau,F.C. Mohlin, S.B. Nabuurs, F.E. Schoenmaker-Koller, D. Smailhodzic,P.A. Campochiarro, D.J. Zack, M.R. Duvvari, B. Bakker, C.C. Paun,C.J.F. Boon, A.G. Uitterlinden, S. Liakopoulos, B.J. Klevering,S. Fauser, M.R. Daha, N. Katsanis, C.C.W. Klaver, A.M. Blom,C.B. Hoyng, A.I. den Hollander, Radboud University Nijmegen MedicalCenter, Department of Ophthalmology, Nijmegen, Netherlands

08:45 99 A prevalent CFHR1/FH reverse hybrid gene in aHUS patientsinduces deregulation of the alternative pathwayL.T. Roumenina, L. Strain, D. Deury, N. Merle, L. Halbwachs-Mecarelli, T. Goodship, V. Fremeaux-Bacchi, INSERM U872,Cordeliers research center, Paris, France

09:00 130 Complement deregulation in Dense Deposit Disease by a novelCFHR2-CFHR5 hybrid proteinQ. Chen, M. Wiesener, H.U. Eberhardt, A. Hartmann, M. Kirschfink,K. Amann, M. Buettner, T. Goodship, C. Hugo, C. Skerka, P.F. Zipfel,Leibniz Institute for Natural Product Research and Infection Biology,Jena, Germany

09:15 104 Blocking C5aR with C5aR antagonist protects mice fromuropathogenic Escherichia coli-induced kidney infectionG.-D. Cai, N. Wang, T. Zhang, Y. Wei , Z.-F. Li , W. Zhou, K. Li, 1Xi'anJiaotong University, Core Research Laboratory, The Second AffiliatedHospital, School of Medicine, Xi'an, China

09:30 135 Differentiating Dense Deposit Disease and C3 Glomerulonephritisby biomarker profilingR. Smith, Y. Zhang, B. Martin, N. Meyer, D.W. Shao, X. Xaio,C. Nester, University of Iowa, Iowa City, United States

09:45 73 Screening of genes encoding complement (regulatory) proteins in patients with C3 glomerulopathyD. Westra, L.P. van den Heuvel, J.A.E. van Wijk, J.F.M. Wetzels,N.C.A.J. van de Kar, Radboud University Medical Centre, PediatricNephrology, Nijmegen, Netherlands

10:00 13 Shiga toxin 2 binds and modulates complement regulatoryproteins in the fluid phase and on host cell membranesD. Orth-Höller, S. Ehrlenbach, A. Rosales, K. Poolpol, P.F. Zipfel,C. Skerka, S.R. de Córdoba, D. Karpman, R. Würzner, InnsbruckMedical University, Innsbruck, Austria


Plenary Lecture

10:45 The STEC HUS Epidemic in Germany 2011: Lessons fromthe Hamburg Nephrology Clinic

Rolf Stahl, Hamburg, Germany

Session V # Complement and Disease II

11:30 149 C5a induces recruitment of STRO-1 pulp progenitor cells:possible role in the regenerative process after dentin/pulp injuryF. Chmilewsky, C. Jeanneau, P. Laurent, M. Kirschfink, I. About, Aix-


Marseille Université, CNRS, ISM UMR, Marseille, France11:45 12 C3 dysregulation due to factor H deficiency is MASP-1/3

independent in vivoM.M. Ruseva, M. Takahashi, T. Fujita, M. Pickering, Imperial CollegeLondon, Medicine, London, United Kingdom

12:00 21 Functional analysis of a complement polymorphism (rs17611)associated with Rheumatoid ArthritisJ. Giles, B.P. Morgan, C.L. Harris, Cardiff University, Infection andImmunity, Cardiff, United Kingdom

12:15 65 Mechanism of neutrophil dysfunction: neutrophil serineproteases cleave and inactivate the C5a ReceptorC. van den Berg, D. Tambourgi, H. Clark, J. Hoong, B. Spiller,E. McGreal, Cardiff University, Molecular and experimental Medicine,Cardiff, United Kingdom

12:30 100 Complement receptor type 2 is crucial in mediatingneuroinflammation and neuropathology after experimental closedhead injury in miceM. Neher, C. Keene, M. Rich, S. Weckbach, A. Bolden, J. Losacco,V.M. Holers, P. Stahel, Denver Health Medical Center, Departments ofOrthopaedics and Neurosurgery, Denver, United States

12:45 Lunchbox

13:30 Afternoon program - Visit to Weimar (Lunch box)19:00 Conference dinner


Tuesday, August 20, 2013


Session VI # Complement Genetics & Therapy

08:30 136 Genetic analysis of the complement and coagulation pathways inatypical Hemolytic Uremic SyndromeR. Smith, F. Bu, T. Mega, N. Meyer, K. Wang , C. Thomas, C. Nester,University of Iowa, Iowa City, United States

08:45 35 High density CGH array for the identification and characterization ofgenomic rearrangements in the RCA gene clusterJ.M. Garcia-Fernandez, S. Pinto, S.R. de Cordoba, Centro deInvestigaciones Biologicas and Consejo Superior de InvestigacionesCientificas (CSIC), Medicina Celular y Molecular, Madrid, Spain

09:00 156 Prevalence of C3 mutations in primary membranoproliferativeglomerulonephritisE. Valoti, E. Bresin, R. Piras, M. Alberti, A. Sorosina, R. Donadelli,G. Remuzzi, M. Noris, IRCCS Istituto di Ricerche Farmacologiche MarioNegri, Ranica, Bergamo, Italy

09:15 31 Identification of novel intracellular differential spliced variants ofMASP1L. Munthe-Fog, P. Schjerling, M.-O. Skjoedt, T. Krag, H.O. Madsen,J. Vissing, H. Lauersen, M. Kjaer, P. Garred, Rigshospitalet, Laboratory ofMolecular Medicine, Copenhagen N, Denmark

09:30 77 Mannose binding lectin is associated with injured vessels in clinicaland experimental traumatic brain injury and its deletion is protectiveF. Orsini, L. Longhi, D. De Blasio, S. Parrella, R. Zangari, F. Ortolano,N. Stocchetti, M.-G. De Simoni, Mario Negri Institute-IRCCS, Neuroscience,Milan,Italy

09:45 148 Eculizumab drug monitoring in serum and urine opens new insightsinto therapy of complement-mediated nephropathiesC. Wehling, O Amon, B. Hohenstein, L. Pape, M. Bommer, M. KirschfinkUniversity of Heidelberg, Heidelberg, Germany

10:00 194 Transient therapeutic inhibition of MASP-2 dependent lectin pathwayfunctional activity provides a significant degree of protection fromischaemia/reperfusion injury in models of myocardial infarction andstrokeE. Chrysanthou, J. Clark, N.J. Lynch, T. Dudler, N.J. Samani, M. Marber,G. Demopulos, W.J. Schwaeble, University of Leicester, Leicester, UnitedKingdom


Session VII # Complement and Adaptive Immunity & Inflammation

10:45 171 Evidence for intracellular complement activation vital to Th1 immunityM.K. Liszewski, M. Kolev, G. Le Friec, M. Leung , P.M. Bertram,M. Pickering, C. Drouet, S. Meri, T.P. Arstila, P.T. Pekkarinen,T. Reinheckel, S.R. de Cordoba, B. Afzali B, J.P. Atkinson, C. Kemper,Washington University, Saint Louis, United Kingdom

11:00 172 C5L2-mediated signals control contraction of human Th1 responsesA. Fara, J. Summer, M. Kolev, C. Roberts, K.T. Lappegård, P. Garred,M. Arno, P. Lavender, J.D. Lambris, T.E. Mollnes, J. Köhl, C. Kemper,King’s College London, MRC Centre for Transplantation-Division ofTransplant Immunology and Mucosal Biology, London, United Kingdom

11:15 14 The innate pathogen recognition molecule ficolin-1 exhibit differentialbinding to leukocyte subsets, providing a novel link between innateand adaptive immunityN. Genster, Y.J. Ma, L. Munthe-Fog, P. Garred, Laboratory of Molecular


Medicine, Copenhagen, Denmark11:30 123 P-I snake venom metalloproteinase is able to activate the complement

system by direct cleavage of the cascade central componentsG. Pidde-Queiroz, F. Carlos Magnoli, F.C.V. Portaro, S.M.T. Serrano,C.W. van den Berg, D.V. Tambourgi, Butantan Institute, Immunochemistry ,São Paulo, Brazil

11:45 54 Envenomation by African adders (Bitis ssp) induces complementactivationD. Paixão-Cavalcante, W. Dias da SIlva, D. Vilarinho Tambourgi, ButantanInstitute, Immunochemistry, São Paulo, Brazil

12:00 147 Cholesterol crystals induce inflammasome activation and cytokinerelease in a complement-dependent mannerE. Samstad, N. Niyonzima, S. Nymo, M. Aune, L. Ryan, O.-L. Brekke,Ø. Sandanger, P. Aukrust, J.K. Damås, E. Latz, J.D. Lambris, T.E. Mollnes,T. Espevik, Norwegian University of Science and Technology, Trondheim,Norway

12:15 27 Potential roles for local adipocytes and fibroblasts in activation of thealternative pathway of complement in inflammatory arthritis in miceW.P. Arend, G. Mehta, A.H. Antonioli, M. Takahashi, K. Takahashi,G.L. Stahl, V.M. Holers, N.K. Banda University of Colorado Denver, Schoolof Medicine, Aurora, United States

12:30 – 14:00 Lunch

13:00 – 14:00 ECN Board Meeting14:00 – 15:30 Postersession II (Posters with odd numbers)

15:30 – 16:30 Award of the ECN Medals

Session VIII # Structure and Function of Complement Proteins

16:30 33 Structural insight on the recognition of complement activator boundopsonins by complement receptor 3G. Bajic, L. Yatime, T. Vorup-Jensen, G.R. Andersen, Aarhus University,Molecular Biology and Genetics, Aarhus, Denmark

16:45 26 Electron microscopy of properdin in complex with C3bBb convertaseM. Alcorlo, S.R. de Cordoba, O. Llorca, Spanish National ResearchCouncil, Centro de Investigaciones Biologicas (CIB), Madrid, Spain

17:00 94 Structure of C1-antibody complexes determined by cryo-electrontomographyC.A. Diebolder, R.I. Koning, A.J. Koster, P. Gros, Utrecht University, Crystaland Structural Chemistry, Bijvoet Center for Biomolecular Research,Department of Chemistry, Utrecht, Netherlands

17:15 192 Further insights into modulation of complement activation by thefactor H-related family of proteinsJ. Caesar, E. Goicoechea de Jorge, E. Chittock, T. Malik, P. Ward,M. Pickering, S. Lea, University of Oxford, Sir William Dunn School ofPathology, Oxford, United Kingdom

17:30 124 Plasma-derived MBL shows direct interaction with C1-inhibitorM. Keizer, N. Brouwer, A. Kamp, M. van de Wetering, D. Wouters,T. Kuijpers, Sanquin Research and Landsteiner laboratory,Immunopathology, Amsterdam, Netherlands

17:45 78 Dendritic cells express alternative pathway components properdinand factor H: differential regulation by Interferons & IL-27J. O`Flynn, K. Dixon, N. Klar Mohamad, M.R. Daha, C. van KootenLeiden University Medical Centre, Nephrology, Leiden, Netherlands


Wednesday, August 21, 2013


Session IX # Complement and Coagulation

08:30 62 Key role of tissue factor in complement-mediated coagulationactivation by Escherichia coli and LPSO.-L. Brekke, A. Landsem, H. Fure, D. Christiansen, E. Waage-Nielsen,J.D. Lambris, T.E. Mollnes, University of Tromsø, Institute of ClinicalMedicine, Tromsø, Norway, Norway

08:45 75 Red blood cell-derived microvesicles amplify systemic inflammationin endotoxemic mice by thrombin-dependent activation ofcomplementD. Zecher, A. Cumpelik, J. Schifferli, Basel University Hospital, Dept. ofResearch and Dept. of Nephrology, Basel, Switzerland

09:00 102 Von Willebrand factor interacts with Factor H and enhances itscofactor activityJ. Rayes, L. Roumenina, Y. Repressé, J. Dimitrov, M. Ing, S. Delignat,L. Halbwachs-Mecarelli, A. Borel-Derlon, S. Kaveri, V. Fremeaux-Bacchi,S. Lacroix-Desmazes, INSERM U872, Cordeliers research center, Paris,France

09:15 30 Von Willebrand factor binds to surface-bound C1q in vitroR. Kölm, M. Schaller, D. Vanhecke, J.A. Kremer Hovinga, E. Khanicheh,B.A. Kaufmann, M. Trendelenburg, University Hospital Basel, Departmentof Biomedicine, Basel, Switzerland

09:30 204 Plasma concentrations of C3 and C4 are linked to cardiovascular riskfactors and to the metabolic syndromeB. Nilsson, K. Nilsson Ekdahl, L. Lind, Uppsala University, Dept. ofImmunology, Genetics and Pathology (IGP), Rudbeck Laboratory C5:3,Uppsala, Sweden

09:45 8 Treatment with Eculizumab of patients with chronic inflammatorydemyelinating polyradiculoneuropathy (CIDP) associated with CD59deficiencyD. Mevorach, Hadassah-Hebrew University, Rheumatology ResearchCenter, Jerusalem, Israel

10:00 60 Enhanced complement activation by low-avidity anti-citrullinatedprotein antibodies (ACPA) possibly explains the association withenhanced joint destruction in rheumatoid arthritisT. Bouwens, P. Suwannalai, U. Scherer, A. van der Helm-van Mil,D. van Schaardenburg, T. Huizinga, R. Toes, L. Trouw, LUMC,Rheumatology, Leiden, Netherlands


Session X # Complement Diagnostics and Therapy

10:45 131 Regulation of complement-dependent cytotoxicity by microRNAY. Hillman, N. Shomron, Z. Fishelson, Tel Aviv University, Cell andDevelopmental Biology, Tel Aviv, Israel

11:00 187 Optimisation and evaluation of the next generation of triple-targetedimmune inhibitors from the “mini-FH class” of engineeredcomplement inhibitorsC. Schmidt, Z.Lin, A. Risitano, R. DeAngelis, T. Simmet, D. Ricklin,J.D. Lambris, Ulm University, Institute of Pharmacology of NaturalProducts & Clinical Pharmacology, Ulm, Germany

11:15 198 Phase 1 clinical trial of novel complement C5 inhibitor coversinW. Weston-Davies, J.-P. Westwood, M. Nunn, Varleigh ImmunoPharmaceuticals, London, United Kingdom


11:30 80 Novel monoclonal antibodies to C3d for non-invasively monitoringthe activity of complement in glomerular and retinal diseaseB. Rohrer, L. Kulik, L.M. Mitchell, D.E. Hourcade, J.P. Hannan,B. Coughlin, A.S. Woodell, M.C. Pickering, V.M. Holers, J.M. Thurman,Medical University of South Carolina, Ophthalmology, Charleston, SC,United States

11:45 188 Preclinical development & evaluation of compstatin analogs as anefficient and cost-effective treatment option for paroxysmalnocturnal hemoglobinuriaD. Ricklin, E. Reis, P. Ricci, Y. Huang, Z. Lin, H. Chen, R. Resuello,R. DeAngelis, A. Risitano, J.D. Lambris, University of Pennsylvania,Philadelphia, United States

12:00 55 Complement involvement in periodontitis: Mechanisms andtherapeutic interventionG. Hajishengallis, T. Maekawa, T. Abe, E. Hajishengallis, E. Reis,R. DeAngelis, J.D. Lambris, University of Pennsylvania, Philadelphia,United States

12:15 137 Human FH 6-17 (a mini-FH) restores serum C3 levels in FH-/- miceE.-M. Hunze, T. Barbour, N. Sheerin, M.C. Pickering, K. Marchbank,Newcastle University, Institute of Cellular Medicine, Newcastle upon Tyne,United Kingdom

12:30 45 Complement and Coagulation- Link of Cascade Systems J. LorenzDobó József P. Gál, V. SchroederBern University Hospital, Bern, Switzerland

12:45 AWARDS

13:00 Presentation ICS Meeting 2014, Rio de Janeiro, Brazil

13:15 15th

ECN Meeting 2015

Farewell

program of the 14th european meeting on complement and...

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