proposal presertation 1

8/6/2019 Proposal Presertation 1

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Ph. D. Proposal

Epidemiological study and molecular characterization of

Hydatid Cysts Isolated from human and some

Intermediate Hosts in Sulaimanya Province.

ByAbdullah Ahmed Hama

M.Sc. Biology/ Parasitology 2007

Supervisor

Prof. Wijdan Mero


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IntroductionHydatid disease is a silent zoonotic infection of

man and domestic animals caused by the larvalstage of the small dog tapeworm.

Man becomes infected accidentally by ingesting

the eggs of the parasite through hands, food, drink

or other materials contaminated with dog feces.

The commonest sites of infection are the liver

and lungs. The disease can not be transmitted

between humans or from human to dogs

Introduction


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Hydatid cyst after surgery


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The disease is endemic or hyper endemic in most

of the Middle East countries mainly in communities

where animal husbandry is common.

Ten intra-specific variants, or genotypes (G1-G10),

have been defined based on genetic diversity.

Introduction


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The identification of the strains responsible

for human echinococcosis is nowadays a

significant point which has to be taken into

consideration in order to focus and to adapt

the control measures and the means of

diagnosis.


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The life cycles :

Life cycle involve two mammalian hosts .The adult cestode

inhabits the small intestine of a carnivore and produces eggs

containing infective oncospheres. Either cestode segments

(proglottids) containing eggs or free.

eggs are released from the intestinal tract of the carnivore in to

the environment. After oral uptake of eggs by an intermediate

host animal, a larval stage, the metacestode, develops in

internal organs. Typically, the mature metacestode produces

numerous protoscoleces.

Life cycle


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Echinococcus species life cycle

Life cycle


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Location of the cyst


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Aim of this studyThe objectives of the present study are to :

1. Investigate the status and prevalence of hydatid disease inhuman

and animal in Sulamania province .

2. Determine the risk group of echinococcus by immunological (ELISA)

screening.

3. An attempt to determining dynamic of echinococcus egg

transmission depending on occupation.

4. Determine the common strain which infect human in sulaimania.

5. Molecular characteristic and genetic diversity (genotyping) of

E. granulosus in different district sulaimania.

Aim


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from animal

Sample Collection

Direct M.EHistological confirmation

PCR amplification of CO1 gene and using

specific primer

DNA extraction

Methods and sampling

Epidemiological analysis for 5

year for human and animals

Genetic diversity (genotyping) for human, goats, sheep and cattle

Germinal layerProtoscolecies by centerfugation

1

halabja

sulaimania

kalar

penjween

Saeed sadq

Rania


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Human

Genetic diversity (genotyping) for human, goats,

sheep and cattle

ELISA IgG detection

cyst from liver lung, spleen and other organ by

surgery

NegativePositive

PCR amplification of CO1 gene and

using specific primer

DNA extraction

2

animal husbandry

House wife

Grocer

Shepherd

10 ml Serum from

Butcher

Abattoir worker

D. M. Examination

Germinal layer separated Aspiration of fluid

Hisological conformation

Veterinarian

Control normal person


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End

proposal presertation 1

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