prostate: transverse section. apex bladder neck radical prostatectomy
TRANSCRIPT
Prostate: Transverse section
Apex Bladder neck
Radical Prostatectomy
Radical Prostatectomy
Base/Bladder Neck Apex
Posterior quadrants of alternate slices have been removed and frozen after inking the surface
Lateral aspect
SeminalVesicle
12 3 4 5 6 7
5
4
3
right left
5R 5L
4R 4L
3R 5L
5R5L
4R4L
3R3L
Permanent sectionPermanent section
Permanent section
permanent section
Unfixed tissuefor RNA & DNA
Frozen section
Schematic of how samples of tumor are obtained from a radical prostatectomy specimen, without compromising
sampling or diagnostic pathology data
Frozen section
right
right
left
left
= Prostate carcinoma
Fresh, non-frozen tissuefor flow sorting
Human prostate (lateral view)
SeminalVesicle
12 3 4 5 6 7
5
4
3
right left
5R 5L
4R 4L
3R 5L
5R5L
4R4L
3R3L
Fixed tissue: In-situ hybridization
Frozen tissue:ImmunostainsLaser capture
Obtaining samples of non-tumor tissue from a radical prostatectomy specimen.
Frozen section
right
right
left
left
= Prostate carcinoma
Fresh, non-frozen tissue: cell sorting
Permanent section
Permanent section
Permanent section
This block is snap frozen
(isopentane in liquid N2), within < 20 minutes of
devitalization
Prostate (transverse section)
Normal prostate parenchyma (high magnification)
Basal epithelial cells
Secretory (luminal) epithelial cells
Fibromuscular stromal cells
lymphocytes
fibromuscular stroma
normal glands
Normal prostate parenchyma (low magnification)