protein engineering
DESCRIPTION
Protein structure computational design and engineering. Application to proteins, enzymes, peptidomimetics, peptides, vaccines, antibodiesTRANSCRIPT
![Page 1: Protein Engineering](https://reader035.vdocument.in/reader035/viewer/2022062405/557cbe65d8b42a59078b490f/html5/thumbnails/1.jpg)
Structure-based Protein Design and Engineering
• What can it do for you?
• How it works?
Health, Environment and Technology
Molecular Modelling Mini Tutorial
![Page 2: Protein Engineering](https://reader035.vdocument.in/reader035/viewer/2022062405/557cbe65d8b42a59078b490f/html5/thumbnails/2.jpg)
Protein-based Technologies are widely used in our daily life
Health Environment Technology
Molecular Modelling Mini Tutorial
![Page 3: Protein Engineering](https://reader035.vdocument.in/reader035/viewer/2022062405/557cbe65d8b42a59078b490f/html5/thumbnails/3.jpg)
Proteins Enzymes and Peptides are made of a sequence of aminoacids
1 MASPFALLMV LVVLSCKSSC SLGCDLPETH SLDNRRTLML LAQMSRISPS SCLMDRHDFG 61 FPQEEFDGNQ FQKAPAISVL HELIQQIFNL FTTKDSSAAW DEDLLDKFCT ELYQQLNDLE121 ACVMQEERVG ETPLMNADSI LAVKKYFRRI TLYLTEKKYS PCAWEVVRAE IMRSLSLSTN181 LQERLRRKE
This is the sequence of a well known protein.
Can you guess what it is, what it does and how it performs it function?
Molecular Modelling Mini Tutorial
![Page 4: Protein Engineering](https://reader035.vdocument.in/reader035/viewer/2022062405/557cbe65d8b42a59078b490f/html5/thumbnails/4.jpg)
Protein function can be understoodby its 3D molecular structure
Protein structures can be determined by:
X-ray NMR Homology Modelling
Molecular Modelling Mini Tutorial
![Page 5: Protein Engineering](https://reader035.vdocument.in/reader035/viewer/2022062405/557cbe65d8b42a59078b490f/html5/thumbnails/5.jpg)
Do you have a sequence and want to …
• Know it’s structure
• Understand it’s function
• Improve it’s native properties
• Perform targeted mutations
• Improve structure specific features
Molecular Modelling Mini Tutorial
![Page 6: Protein Engineering](https://reader035.vdocument.in/reader035/viewer/2022062405/557cbe65d8b42a59078b490f/html5/thumbnails/6.jpg)
Bimolecular Modelling can help you!
Active site Protein-proteinaggregation site
Conserved domain
Mobile domainHydrophobicpatch
We model a 3D structure
from your sequence, and provide
detailed structural and dynamic
insights concerning it’s function
Molecular Modelling Mini Tutorial
![Page 7: Protein Engineering](https://reader035.vdocument.in/reader035/viewer/2022062405/557cbe65d8b42a59078b490f/html5/thumbnails/7.jpg)
What about engineering improvedprotein function and structure?
Focus your effort on the most
promising recombinant proteins
predicted by our computer-aided
protein structure design platform
We can perform large scale
aminoacid mutation screening,
so you can focus your effort on
the most promising
recombinant proteins.
Molecular Modelling Mini Tutorial
![Page 8: Protein Engineering](https://reader035.vdocument.in/reader035/viewer/2022062405/557cbe65d8b42a59078b490f/html5/thumbnails/8.jpg)
Molecular Modelling can support your research & development effort on:
• Vaccines
• Antibodies
• Peptides
• Peptidomimetics
• Enzymes
• Other protein-based technologies
Molecular Modelling Mini Tutorial
Dr. Nuno MicaeloResearcher, Aveiro University