ps 2 ppt - general santos hope christian school

8/10/2019 PS 2 PPT - General Santos Hope Christian School

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The Potential of Coconut Husk as

Phytoremediating Agent for Lead andCopper Contaminated Water Tested throughBrine Shrimp Toxicity Assay


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Objective

To adsorb copper and lead in water with the use of coconut husk.


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Procedure

Gathering of coconut husk

Weighing 10 g of coconut husk

Preparing 2 beakers with 200 ml distilled water

Preparing 2mg of lead and copper

Separately mixing lead and copper in both beakers

Serial dilution of lead and copper in six (6) respective test tubes.

Adding Brine Shrimps to both set ups. One set-up for lead and one set-up for copper. Each

set-up was duplicated one with treatment and the other without treatment.

Adding 1.0g cubed shaped coconut husk to treated test tubes.

Data collection (counting the brine shrimp mortality)

Probit Analysis


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Results:

Concentration of

Water No of Brine Shrimp

No. of Brine Shrimp

Dead Percent Kill

Log Concentration

(x)

Empirical

Probit

1 18 14 78 0 5.77

0.1 20 10 50 -1 5.00

0.01 18 8 44 -2 4.85

0.001 19 8 42 -3 4.8

0.0001 20 5 25 -4 4.33

0.00001 23 3 13 -5 3.87

Lethal Concentration of Lead Contaminated Water Without Coconut Husk


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Graphical Presentation of Log Concentration Vs. Empirical Probit Value

Mortality Analysis Lead Contaminated Water Without Coconut Husk

The values in Table 1 is plotted

and a linear regression function

is determined through algebraic

method. The function is being

determined as y = 0.3303x +5.5957 Setting the value of y at

5.0 will represent Lethal

Concentration at which 50% of

the tested organism will die.

The Lc50 of the linear

regression is -1.8035 and

extracting the antilog of x, the

researcher obtained the value

of 0.0157217 mg/L., the

concentration at which 50%

mortality of the experimental

brine shrimp is attained.


6/13

Lethal Concentration of Lead Contaminated Water Treated With Coconut Husk

Concentration of


No. of Brine Shrimp

Dead Percent Kill

Log Concentration

(x) Empirical Probit

1

15

10

67

0

5.44

0.1 16 7 44 -1 4.85

0.01 17 4 24 -2 4.29

0.001 14 2 14 -3 3.92

0.0001 19 2 11 -4 3.77

0.00001 15 1 7 -5 3.52


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Mortality Analysis Lead Contaminated Water Treated With Coconut Husk

The values in Table 2 is plotted and a

linear regression function is

determined through algebraic

method. The function is beingdetermined as y = 0.3774x + 5.2419

Setting the value of y at 5.0 will

represent Lethal Concentration at

which 50% of the tested organism will

die. The Lc50 of the linear regression

is -0.64096 and extracting the antilog

of x, the researcher obtained thevalue of 0.2286 mg/L., the

concentration at which 50% mortality

of the experimental brine shrimp is

attained.


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Lethal Concentration of Copper Contaminated Water Without Coconut Husk

Concentration of


No. of Brine Shrimp

Dead Percent Kill

Log Concentration


1 21 13 62 0 5.31

0.1 25 12 48 -1 4.95

0.01 20 10 50 -2 5.00

0.001 26 9 35 -3 4.62

0.0001 25 8 32 -4 4.53

0.00001 22 4 18 -5 4.08


9/13


Mortality Analysis Copper Contaminated Water Treated Without Coconut Husk


linear regression function is

determined through algebraic method.

The function is being determined as y

= 0.2226x + 5.3048 Setting the valueof y at 5.0 will represent Lethal

Concentration at which 50% of the

tested organism will die. The Lc50 of

the linear regression is -1.3693 and

extracting the antilog of x, the

researcher obtained the value of0.042727 mg/L., the concentration at

which 50% mortality of the

experimental brine shrimp is attained


10/13

Lethal Concentration of Copper Contaminated Water Treated With Coconut

Husk

Concentration of


No. of Brine Shrimp

Dead Percent Kill

Log Concentration


1

11

7

64

0

5.36

0.1 13 6 46 -1 4.90

0.01 11 5 45 -2 4.87

0.001 12 2 17 -3 4.05

0.0001

12

1

8

-4

3.59

0.00001 14 1 7 -5 3.52


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Mortality Analysis Copper Contaminated Water Treated With Coconut Husk


linear regression function is determined

through algebraic method. The function is

being determined as y = 0.3986x +

5.3781 Setting the value of y at 5.0 will

represent Lethal Concentration at which

50% of the tested organism will die. The

Lc50 of the linear regression is -0.9486

and extracting the antilog of x, the

researcher obtained the value of0.11257mg/L., the concentration at which

50% mortality of the experimental brine

shrimp is attained.


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Conclusions:

Coconut husk has the potential in eliminating copper, and lead from water.


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1. That the Department of Health will investigate into this fact to confirm this result. If proven

through their effort, then publish the information to people so that they can apply this treatment

to make sure that their water is fit for drinking.

2. That people must seek medical aid if something happened to them after using this treatmentto avoid serious complications.

3. That the same study may be carried out by another researcher to find out the efficiency of

coconut husk in treating other cations from water.

Recommendations:

ps 2 ppt - general santos hope christian school

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