1

Questionnaire

Summary of the main activities of a scientific Organisation

of the Slovak Academy of Sciences

Period: January 1, 2003 - December 31, 2006

I. Formal information on the assessed Organisation:

1. Legal name and address Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4, 040 01

Košice, Slovakia

2. Executive body of the Organisation and its composition

Directoriat name age years in the position

director Assoc. Prof. MVDr. Juraj Koppel,DrSc. 52 1990-

deputy director Assoc. Prof. RNDr. Peter Javorský,DrSc. 56 1990-

scientific secretary Prof. MVDr. Vladimír Kmeť, DrSc. 55 2001-

3. Head of the Scientific Board

Assoc. Prof. MVDr. Štefan Faix, CSc.

4. Basic information about the research personnel i. Number of employees with a university degree (PhD students excluded)

engaged in research and development and their full time equivalent work capacity (FTE) in 2003, 2004, 2005, 2006 and average number during the assessment period

2

ii. Organisation units/departments and their FTE employees with the university degree engaged in research and development

No. FTE No. FTE No. FTE No. FTE No. FTE

organisation in whole 28 26,09 30 27,22 30 27,88 27 26,18 28,75 26,843

unit 1 ###### ######

unit 2 ... ###### ######

###### ######

###### ######

###### ######

###### ######

###### ######

###### ######

###### ######

###### ######

Research staff2003 average200620052004

5. Basic information on the funding

i. Total salary budget1 of the Organisation allocated from the institutional resources of the Slovak Academy of Sciences (SAS) in 2003, 2004, 2005, 2006, and average amount for the assessment period

Salary budget 2003 2004 2005 2006 average

total salary budget (millions of SKK) 9,292 9,192 9,586 10,320 9,598 6. URL of the Organisation’s web site

http://www2.saske.sk/iap/

1 Sum of the brutto salaries without the fund contributions.

3

II. General information on the research and development activity of

the Organisation: 1. Mission Statement of the Organisation as presented in its Foundation

Charter The Institute was founded in 1964 as the Department of Animal Physiology of the

Institute of Experimental Biology and it has become an independent Institute since 1969. The

Institute is dealing with the basic research of animal physiology as the only one in Slovakia.

According the foundation charter the main function and the role of the Institute is the research

and development in veterinary, chemical, biological and agricultural-forestry sciences.

Another important mission of the Institute is the education of PhD students in veterinary

physiology, animal physiology and biochemistry. The Institute has a wide cooperation

network with universities and other research institutes in Slovakia and abroad.

2. Summary of R&D activity pursued by the Organisation during the assessed period, from both national and international aspects and its incorporation in the European Research Area (max. 10 pages)

During the assessed period (2003-2006) the scientific activities of Institute were

focused on research into animal digestive physiology, molecular microbial diagnostics and

GMO, functional foods, selenium metabolism, preimplantation embryo and apoptosis.

The approach oriented to the relationship “nutrition – microorganism – digestion –

host organism” is based on complex problem solving. As the experimental model the rumen

simulating system (Rusitec) was used, and experiments with animals were also performed.

The major aim was to obtain new information about host - microorganism interactions and

about the possibilities of manipulating the physiology of digestion using probiotics,

antimicrobial substances of plant origin, bacteriocines and polyunsaturated fatty acids with

respect to enzyme activities in enterocytes, antioxidant enzymes and to the production of

cytokines and about the role of microorganisms in enteritis. An important part of the research

investigated the possible replacement of feed antibiotics with essential oils (their

antimicrobial, antiinflammatory and immunomodulatory effects) as well as the research of

selenium metabolism and excretion in relation to functional foods.

4

Essential oils and probiotics in projects of the State Program, APVV and VEGA

The most recent results of in vivo experiments (mice and chicken) reveal that the most

effective essential oils with anti-inflammatory effects are mainly cinnamon and rosemary, and

borneol (active compound of Salvia). The results show that administration of cinnamon as a

feed additive could reduce colon damage in TNBS-induced colitis. The decrease of E. coli

translocation from intestine to mesenteric lymphatic nodes was observed after peroral

administration of various cinnamon concentrations in mice. TNBS-induced colitis significantly increased the expression of pro-inflammatory

cytokines IL-1b and IL6, whereas treatment of animals with a combination of thyme and

oregano essential oils significantly decreased the level of cytokines.

The changes of bacterial adherence to intestinal surfaces were also studied in in vitro

experiments on Caco-2 cell lines. Decrease of E. coli adherence to Caco-2 cells after

application of various cinnamon and thyme concentrations was measured. Oregano and

cinnamon also in laboratory conditions had strong antimicrobial activities and represent

selection pressure similar to feed antibiotics. We observed statistically-significant increase in

MIC of commensal E. coli in amikacin, apramycin, streptomycin and neomycin in feces of

chicken fed with cinnamon-added feed in comparison to the control group of chicken.

The probiotic strain Enterococcus faecium EF2019 produces enterocin allotted to the

II. group of bacteriocins (thermo-stable substances) i.e. small peptides with a broad

antimicrobial inhibitory spectrum possessing molecular mass of about 3-10 kDa and with

optimal production in pH range 4-7. This strain also possesses structural genes for the

production of already-known enterocins, e.g. ent A, B and L50B.

Molecular diagnostics of microorganisms and GMO in projects of the State

Program, APVV, FP5 and VEGA

Within the frame of the State Program of Science and Development project

“Alternative ways for replacement of antibiotic–influencing of animal digestion physiology”,

our institute collaborated with the State Veterinary and Food Institute in Nitra using Real-

Time PCR to develop a method for quantification of Mycobacterium paratuberculosis in

cattle feces samples suspected of paratuberculosis, whereever IS 900 sequence was confirmed

in cow feces samples.

5

Several rare isoschisomers were identified from rumen treponemas. Modification

methyltransferases show dual functionality, when some of them participate in regulation of

other cellular processes. However, the dual functionality is exclusive, as methylases of

restriction-modification systems never use the same sequence compared to enzymes with

regulatory functions. For the first time we observed the concurrent presence of adenine

methylation, probably with regulatory function; and cytosine methylation, a part of the

restriction-modification system, recognizing identical GATC sequences in Mitsuokella

multiacida. The presence of two methyl groups in close proximity sterically interferes with

binding of DNA interacting proteins. Statistical analysis of available plasmid sequences, a

natural restriction-modification system target, indicates that there is significant

underrepresentation of GATC sequences in plasmid genomes.

To evaluate the safety of GMO, the potential risk of natural gene transfer from

modified corn (Zea mays) to environmental microorganisms was analyzed. A set of “marker

rescue” vectors was developed for application in bacterial species Erwinia stuartii. The results

obtained indicate that while easily transformable under in vitro conditions, no transfer was

detected from modified Bt176 corn to phytopatogenetic E. stuartii strain during long-term

experiment trials. Based on this observation natural gene transfer from modified corn to

environmental microorganisms does not contribute any measurable risks.

In the framework of the FP5 ERCULE project the first European rumen ciliate

collection was established. A rapid molecular method for identification of rumen protozoa

was developed based on ARDRA analysis. Using DGGE analysis the variability of rumen

protozoa was analyzed. We have characterized archaebacteria symbiotically living with ciliate

cells.

In the subsequent FP5 CIMES project, a unique ciliate genome organization was used

for studying the safety of genetically-modified organisms. We have shown that rumen ciliate

possesses a unique genome organization with macronuclear “midi”chromosomes ranging in

size from 25 -100 kbp. Analysis of bacterial populations associated with protozoa indicated

that even a single protozoal cell is very complex microbial ecosystem, and that changes in

associated bacterial populations help protozoa deal with environmental stress. The most

important finding was that despite the existence of horizontal gene transfer on a historical

scale, transfer frequencies in the real world and gene transfer associated risks are negligible.

6

Functional foods (starter culture, PUFA and selenium)

In the framework of the FP5 project “Tradisausage”, decarboxylase-negative strain

Staphylococcus xylosus S03/1M/1/2 was selected with sufficient adhesive ability to human

mucosa. This strain was applied as a new protective additive during fermented sausage

processing. Sufficient counts of the strain S03/1M/1/2 were enumerated in the final product

even after 4 weeks of ripening. Moreover, the sensoric properties as well as technological

quality of the final product were well maintained, and in addition spoilage bacteria were

reduced.

The supplementation (5%, wt.wt-1) of microbial oil (MO), borage oil (BO) and

evening primrose oil (EPO) to the diet consisting of 60% hay and 40% barley on lipid

metabolism in artificial rumen (Rusitec) was studied. All oils contained gamma- linolenic acid

(GLA) – MO (8.4%), BO (23.7%) and EPO (9.2%) respectively. The supplementation of the

oils increased the concentrations of trans C18:1 isomers and stearic acid. CLA concentration

increased 2.3, 1.2, and 2.1 times with MO, EPO and BO respectively. Moreover, TVA

concentration in effluent was increased by the oil supplements. Finally, the supplementation

of the oils MO, EPO and BO to mixed diet in vitro caused incomplete biohydrogenation of

polyunsaturated fatty acids (PUFA), and was characterized by higher TVA concentration

and higher TVA/C18:0 ratio in experimental diets.

Experiments on chickens fed with diets enriched with various forms of selenium

showed that intake of organic Se (selenized yeast) resulted in significantly higher retention of

Se in body deposits (mainly in striated muscle) than the inorganic form of this essential

microelement. The major contribution of using selenized yeast in animal feedstuffs is the

increased transfer of this essential element via the food chain to the human population. In

cooperation with the companies Novogal a. s. Dvory nad Žitavou and PG Trade s.r.o.

Komárno, we contributed to the development of selenized egg production as functional food.

The eggs of size L from laying hens fed diets enriched with selenized yeast reached a total Se

content per egg of 18-20 µg after only 6 weeks of treatment. The integration of organic

selenium into the food chain could aid in improving the health of the human population,

which is subject to widespread selenium deficiency in Slovakia as well as in the majority of

EU countries.

7

Intestinal physiology in VEGA projects and FP5

Within the project focusing on investigation of functional maturation of the small

intestine in relation to early nutritional events, it was found that postnatal nutritional

experiences (overnutrition or undernutrition) might be a predisposing factor involved in long-

term alteration of the small intestine function and energetic metabolisms, as well as in somatic

development. After high-fat diet feeding in the subsequent periods of these animals’ life,

permanently increased small intestinal alkaline phosphatase, hypertrophy of intestinal mucose

(increased protein/DNA ratio) as well as atendency to increased body fat accretion were

observed. These long-term biochemical and functional changes of the small intestine

associated with alterations in efficiency of food digestion could be considered as a significant

factor in the development of obesity. From this point of view the obtained results on early

nutrition may be important for better understanding the function of the small intestine in the

etiology of obesity and the occurrence of susceptibility or resistance to diet-induced obesity in

later life.

Selenium metabolism in VEGA projects

Experiments on laying hen hybrid Shaver Starcross 288 showed that dietary

supplementation with selenized yeast is more effective in maintenance of both antioxidant and

selenium status of birds than with selenite. The positive consequence of the body formation of

selenomethionine deposits in chickens appears to be a high mobility of selenium during stress

and/or diseases of birds. Effects of selenium on chicken immunocompetent cells assessed by

immunohistochemical analysis of differentiation of T lymphocytes CD3+, CD4+ and CD8 in

duodenal lamina propria, caecal tonsils and bursa of Fabricius showed positive correlation

with Se intake.

The excretion routes of selenium were investigated in sheep after intravenous selenite

loading. The most quantitatively significant Se excretion was by found in urine, but the

increase in Se fecal concentration suggested also secretion of some unidentified Se-

metabolites into the digestive tract. The delayed peak of selenium urinary excretion (3 hours

after the end of selenite infusion) suggests unspecific bindings of dihydrogen selenide and/or

other Se metabolites to plasma albumin with their later release and subsequent excretion.

8

The principal characteristics of renal selenium excretion in sheep after selenite

infusion were established in standard clearance experiments. Despite extremely rapid

selenium excretion due to 7-fold increased Se level in plasma, the factor limiting its

glomerular filtration appears to be some unspecific bindings of intermediate selenite

metabolites on plasma proteins. Nevertheless, the rapid Se elimination after selenite

administration suggests low bioavailability of this inorganic source of selenium for farm

animals.

Apoptosis and preimplantation embryo in VEGA and APVV projects

In our study on G protein-coupled receptors, we were the first to show that several

types of adrenergic receptors are expressed in mouse preimplantation embryos and ovulated

oocytes. The results of our in vitro experiments showed that stimulation of the identified

adrenergic receptors by specific ligands can influence cell-proliferative activity in the

preimplantation embryo. Epinephrine and norepinephrine can then, via these receptors,

directly affect the embryo development in very early pregnancy – before embryo implantation

into the uterus.

The complete profile of the occurrence of spontaneous and induced apoptosis was

compiled at all stages of preimplantation development of mouse and rabbit embryos.

Apoptotic processes evaluated in blastocysts generally displayed similar characteristics as

apoptotic processes described in fully-differentiated somatic cells. A relatively high portion of

blastocysts derived in vivo or produced in vitro showed the presence of at least one apoptotic

cell. The observed apoptotic cells showed the typical morphological and biochemical features

of apoptosis, but their incidence was species-specific – rabbit blastocysts showed significantly

lower frequency of spontaneous apoptosis than mouse blastocysts. Nevertheless, blastocysts

of both species were able to respond to the presence of an apoptotic inductor, and showed

significantly higher incidence of apoptotic cells. During early development, preceding the

blastocyst stage, the appearance of spontaneous apopotosis was sporadic and its presence was

observed only at stages following embryonic genome activation (EGA). The susceptibility of

embryos to the apoptotic inductor in the early stages was much lower – the inductor did not

increase the incidence of apoptotic embryos or apoptotic cells. However, it had a negative

effect on embryo growth and triggered earlier appearance of some apoptotic features.

9

We were also the first to show that DNA helicase II synthesis (controls nucleic acids

metabolism) drop to 40% level at the end of the growing stage, immediately before meiosis

resumption. During the growing period DNA helicase II is localized in the nucleolus, but at

the end of that stage it is re-localized to the nucleolus periphery. We showed for the first time

that DNA helicase II location is different when comparing growing oocytes with somatic cells

in the analogical cell cycle stage. During resumption of meiosis, the oocyte nucleus breaks

down and chromatin condenses. These events bear direct relation to activation of cyclin-

dependent kinase CDK1. Our results showed that Akt/PKB (protein kinase B) plays a key role

in the pathway downward to CDK1 to be activated immediately before meiosis resumption.

These primary results of basic science could be used later for a new biotechnology strategy of

assisted reproduction.

During the assessed period (2003-2006) the cited original results confirm the leading

role of Institute on national and international level in the research of animal digestive

physiology and related molecular microbial diagnostics, as well as in the research of

functional foods, selenium metabolism, preimplantation embryo and apoptosis.

III. Partial indicators of the main activities: 1. Research output

i. List of the selected publications documenting the most important results of basic research. Total number of publications in the whole assessed period should not exceed the average number of the research employees

[1] LENG, L. - BOBCEK, R. - KURICOVA, S. - BOLDIZAROVA, K. -

GRESAKOVA, L. - SEVCIKOVA, Z. - REVAJOVA, V. - LEVKUTOVA, M. -

LEVKUT, M. Comparative metabolic and immune responses of chickens fed diets

containing inorganic selenium and Sel-PlexTM organic selenium. In Lyons, T. P. and

Jacques, K. A. Nutritional Biotechnology in the Feed and Food Industries.

Notingham: Nottingham University Press, Nottingham 2003. ISBN 1-897676-40-9. p.

131-145.

[2] BOLDIZAROVA, K. - GRESAKOVA, L. - FAIX, S. - LEVKUT, M. - LENG, L.

Urinary selenium excretion in selenite-loaded sheep and subsequent Se dynamics in

10

blood constituents. In: Reproduction, Nutrition and Development, Vol. 43, 2003, p.

385-393.

[3] FABIAN, D. – REHAK, P. – CZIKKOVA, S. - ILKOVA, G. – BARAN, V. –

KOPPEL, J. Induced cell death of preimplantation mouse embryos cultured in vitro

evaluated by comet assay. In Theriogenology. Vol. 60, 2003, p. 691-706.

[4] STYRIAK, I. - LJUNGH, Å. Binding of extracellular matrix molecules by enterococci.

In Current Microbiology. Vol. 46, no. 6, 2003, p. 435-442.

[5] BARAN, V. - PAVLOK, A. - BJERREGAARD, B. - WRENZYCKI, C. -

HERMANN, D. - PHILIMONENKO, VV. - LAPATHITIS, G. - HOZAK, P. -

NIEMANN, H. - MOTLIK, J. Immunolocalization of upstream binding factor and

pocket protein p130 during final stages of bovine oocyte growth. In Biology of

Reproduction. Vol. 70, no. 4 (2004), p. 877-86.

[6] FABIAN, D. - ILKOVA, G. - REHAK, P. - CZIKKOVA, S. - BARAN, V. -

KOPPEL, J. Inhibitory effect of IGF-I on induced apoptosis in mouse preimplantation

embryos cultured in vitro. In Theriogenology. Vol. 61, no. 4 (2004), p. 745-55.

[7] KISIDAYOVA, S. - VARADYOVA, Z. Potential of galvanotaxis to separation and

cleaning of rumen ciliates. Journal of Microbiological Methods. Vol. 57, (2004), p.

65-68.

[8] MOZES, S. - SEFCIKOVA, Z. - LENHARDT, L. - RACEK, L. Effect of

adrenalectomy on the activity of small intestine enzymes in monosodium glutamate

obese rats. In Physiological Research. Vol. 53, no. 4 (2004), p. 415-22.

[9] MOZES, S. - SEFCIKOVA, Z. - LENHARDT, L. - RACEK, L. Obesity and changes

of alkaline phosphatase activity in the small intestine of 40- and 80-day-old rats

subjected to early postnatal overfeeding or monosodium glutamate. In Physiological

Research. Vol. 53, no. 2 (2004), p.177-86.

[10] PIKNOVA, M. - FILOVA, M. - JAVORSKY, P. - PRISTAS, P. Different

restriction and modification phenotypes in ruminal lactate-utilizing bacteria. In FEMS

Microbiology Letters. Vol. 236, no. 1 (2004), p. 91-5

[11] STROMPFOVA, V. - LAUKOVA, A. - OUWEHAND, A. C. Selection of

enterococci for potential canine probiotic additives. In Veterinary Microbiology. Vol.

100, no. 1-2 (2004), p. 107-114.

[12] CIKOS, S. - VESELA, J. - ILKOVA, G. - REHAK, P. - CZIKKOVA, S. -

KOPPEL, J. Expression of beta adrenergic receptors in mouse oocytes and

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preimplantation embryos. In Molecular Reproduction and Development. Vol. 71,

(2005), p. 145-153.

[13] FABIAN, D. - KOPPEL, J. - MADDOX-HYTTEL, P. Apoptotic processes

during mammalian preimplantation development. In Theriogenology. Vol. 64, (2005),

p. 221-231.

[14] FAIX, S. - FAIXOVA, Z. - BOLDIZAROVA, K. - JAVORSKY, P. The effect

of long-term high heavy metal intake on lipid peroxidation of gastrointestinal tissue in

sheep. In Veterinary Medicine-Czech. Vol. 50, (2005), p. 401-405.

[15] JALC, D. - SZUMACHER - STRABEL, M. - POTKANSKI, A. - CIESLAK,

A. Effect of sunflower, linseed and fish oils on the production of trans fatty acids in

vitro. In Berliner und Munchener tierarztliche Wochenschrift. Vol. 118, (2005), p.

430-435

[16] JALC, D.- POTKANSKI, A. - SZUMACHER - STRABEL, M. - CIESLAK,

A. – CERTIK, M. Effect of microbial oil, evening primrose oil and borage oil on

rumen fermentation in vitro. In Veterinary Medicine –Czech. Vol. 50, (2005), p. 480-

486

[17] KISIDAYOVA, S. - VARADYOVA, Z. - MICHALOWSKI, T. -

NEWBOLD, C. J. Regeneration of cryoresistance of in vitro rumen ciliate cultures. In

Cryobiology. Vol. 51, (2005), p.76-84.

[18] PETROVIC, V. - BOLDIZAROVA, K. - FAIX, S. - KOWALCZYK, J. -

CZAUDERNA, M. - MELLEN, M. - LENG, L. Excretion routes and distribution of

selenium in sheep tissues after selenite loading. In Journal of Animal and Feed

Sciences. Vol. 14, (2005), p. 303-306.

[19] PIKNOVA, M. - JAVORSKY, P. - PRISTAS, P. Multiple restriction-

modification systems are present in rumen treponemes. In FEMS Microbiology Letters.

Vol. 251, no. 1 (2005), p. 99-103

[20] PRISTAS, P. - PIKNOVA, M. Underrepresentation of short palindromes in

Selenomonas ruminantium DNA: evidence for horizontal gene transfer of restriction and

modification systems. In Canadian Journal of Microbiology. Vol. 51, no. 4 (2005), p.

315-318

[21] SPRINCOVA, A. - JAVORSKY, P. - PRISTAS, P. pSRD191, a new member of

RepL replicating plasmid family from Selenomonas ruminantium. In Plasmid. Vol. 54,

no 1 (2005), p. 39-47.

12

[22] VARADYOVA, Z. - BARAN, M. - ZELENAK, I. Comparison of two in vitro

fermentation gas production methods using both rumen fluid and faecal inoculum

from sheep. In Animal Feed Science and Technology 123-124, 2005, 81-94.

[23] BARAN, V. - KOVAROVA, H. - KLIMA, J. - HOZAK, P. - MOTLIK, J.

Re-localization of nuclear DNA helicase II during the growth period of bovine

oocytes In Histochemistry and Cell Biology. Vol. 125, (2006), p.155-164.

[24] FABIAN, D. - SABOL, M. - DOMARACKA, K. - BUJNAKOVA, D.

Essential oils-their antimicrobial activity against Escherichia coli and effect on

intestinal cell viability. In Toxicology in vitro. Vol. 20, no. 8 (2006), p. 1435-45.

[25] HOROSOVA, K. - BUJNAKOVA, D.- KMET, V. Effect of Lactobacilli on E.

coli Adhesion to Caco2 Cells in Vitro. In Folia Microbiologica. Vol. 51, no.4 (2006), p.

281–282.

[26] HOROSOVA,K. - BUJNAKOVA, D.- KMET, V. Effect of Oregano Essential

Oil on Chicken Lactobacilli and E. coli. In Folia Microbiologica. Vol. 51, no.4 (2006) p.

278–280.

[27] SIMONOVA, M. - STROMPFOVA, V. - MARCINAKOVA, M. -

LAUKOVA, A.-VESTERLUND, S. - MORATALLA, M.L. - BOVER-CID, S. -

VIDAL-CAROU, C. Characterization of Staphylococcus xylosus and Staphylococcus

carnosus isolated from Slovak meat products. In Meat Science. Vol. 73, no. 4 (2006),

p. 559-564.

[28] STROMPFOVA, V. - MARCINAKOVA, M. - SIMONOVA, M. - BOGOVIC-

MATIJASIC, B. - LAUKOVA, A. Application of potential probiotic Lactobacillus

fermentum AD1 strain in healthy dogs. In Anaerobe. Vol. 12 , no. 1 (2006), p. 75-79.

[29] VARADYOVA, Z. - KISIDAYOVA, S. - MIHALIKOVA, K. - BARAN, M.

Influence of natural magnesium sources on the in vitro fermentation and protozoan

population in the rumen fluid collected from sheep. In Small Ruminant Research, Vol.

61, (2006), p. 63-71.

ii. List of monographs/books published abroad

[1]

[2] …

iii. List of monographs/books published in Slovakia

13

[1] KACANIOVA, M. - BOBCEK, R. - KMET, V. - ANGELOVICOVA, M. Kŕmne doplnky

ako nahrada antibiotik a ďalsie aplikacie. Nitra: SPU, 2005, 78 s. ISBN 80-8069-589-

X.

iv. List of other scientific outputs specifically important for the Organisation

[1]

[2] …

v. Table of research outputs Table Research outputs shows research outputs in number of specified entries; these

entries are then divided by FTE employees with a university degree (from Tab. Research

staff) for all Organisation at the respective year; finally these entries are divided by the

total salary budget (from Tab. Salary budget).9

14

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chapters in monographs, books published abroad 1 0,04 0,11 0 0,00 0,00 0 0,00 0,00 0 0,00 0,00 1 0,3 0,01 0,03

chapters in monographs, books published in Slovakia

0 0,00 0,00 0,00 0,00 0,00 0,00 0,00 0,00 0 0,0 0,00 0,00

CC publications 31 1,19 3,34 37 1,36 4,03 36 1,29 3,76 42 1,60 4,07 146 36,5 1,36 3,80

scientific publications indexed by other databases (CAB)

4 0,15 0,43 7 0,26 0,76 4 0,14 0,42 4 0,15 0,39 19 4,8 0,18 0,49

scientific publications in other journals 1 0,04 0,11 0,00 0,00 2 0,07 0,21 0,00 0,00 3 1,5 0,06 0,16

publications in proc. of international scientific conferences

9 0,34 0,97 16 0,59 1,74 22 0,79 2,30 22 0,84 2,13 69 17,3 0,64 1,80

publications in proc. of nat. scientific conferences 2 0,08 0,22 1 0,04 0,11 0,00 0,00 7 0,27 0,68 10 3,3 0,12 0,35

active participations at international conferences 30 1,15 3,23 25 0,92 2,72 39 1,40 4,07 41 1,57 3,97 135 33,8 1,26 3,52

active participations at national conferences 1 0,04 0,11 10 0,37 1,09 10 0,36 1,04 13 0,50 1,26 34 8,5 0,32 0,89

total

Research outputs

2003 2004 2005 2006

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vi. Renormalized publications2 Renormalized publications = number of CC publications in the given year times

authorship’s portion of the Organisation times the journal impact factor in 2005 divided by

the median impact factor in the research field

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Renormalized publications 15,6 0,60 1,68 20,3 0,74 2,21 17,8 0,64 1,86 12,9 0,49 1,25

2006

Renormalised publications

2003 2004 2005

vii. Standard manuscript page count3

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page count 0 0,0 0,0 0 0,0 0,0 0 0,0 0,0 0 0,0 0,0

2006

Standard manuscript page count

2003 2004 2005

viii. List of patents and patent applications The World Intellectual Property Organization WIPO

[1] Gregor P, Harris N, Koppel J: Methods of screening for anti-inflammatory drugs and

use thereof. Publication number: WO2004059278, Publication date: 2004-07-15.

[2] Gregor P; Harris N; Koppel J; Zhuk R. Pharmaceutical compositions comprising

thieno[2,3-c]pyridine derivatives and use thereof. Publication number:

WO2004069149, Publication date: 2004-08-19.

2 This information is required only from the Organisations of the Section 2 of the Slovak Academy of Sciences. 3 This information is required only from the Organisations of the Section 3 of the Slovak Academy of Sciences.

16

[3] Gregor P; Harris N; Koppel J; Zhuk R. Pharmaceutical compositions comprising anti-

inflammatory quinazolinecarboxamide derivatives. Publication number:

WO2005089068, Publication date: 2005-09-29.

ix. Supplementary information and/or comments on the scientific output of the

Organisation

2. Responses to the scientific output Table Citations shows specified responses to the scientific outputs; these entries are

then divided by the FTE employees with a university degree (from Tab. Research staff)

for all Organisation at the respective year; finally these entries are divided by the total

salary budget (from Tab. Salary budget).

num

ber

No.

/ FT

E

No.

/ sa

lary

bud

get

num

ber

No.

/ FT

E

No.

/ sa

lary

bud

get

num

ber

No.

/ FT

E

No.

/ sa

lary

bud

get

num

ber

No.

/ FT

E

No.

/ sa

lary

bud

get

num

ber

aver

aged

num

ber

per y

ear

av. N

o. /

FTE

av. N

o. /

sala

ry

budg

et

Web of Science 85 3,3 9,1 111 4,1 12,1 91 3,3 9,5 94 3,6 9,1 381 95,3 3,5 39,7

Scopus 0 0,0 0,0 0 0,0 0,0 6 0,2 0,6 3 0,1 0,3 9 2,3 0,1 0,9

ProQuest 0 0,0 0,0 0 0,0 0,0 0 0,0 0,0 13 0,5 1,3 13 3,3 0,1 1,4

in monographs, conf. proceedings and other publications abroad

3 0,1 0,3 1 0,0 0,1 5 0,2 0,5 10 0,4 1,0 19 4,8 0,2 2,0

in monographs, conf. proceedings and other publications in Slovakia

0 0,0 0,0 0 0,0 0,0 0 0,0 0,0 5 0,2 0,5 5 1,3 0,0 0,5

Citations

total2002 2003 2004 2005

17

i. List of 10 top-cited publications and number of their citations in the assessment period

1. PAMPFER S., - VANDERHEYDEN I., - MCCRACKEN J., - VESELA J., - DE HERTOGH R.: Increased cell death in rat blastocysts exposed to maternal diabetes in utero and to high glucose or tumor necrosis factor-α in vitro. Development 124, 1997, 4827-4836. Cited 28 times

2. LUCCHINI F., - KMET V., - CESENA C. COPPI L, BOTTAZZI V, MORELLI L. : Specific detection of a probiotic Lactobacillus strain in faecal samples by using multiplex PCR. FEMS Microbiol. Letters 158, 1998, 273-278 Cited 13 times

3. MOROVSKY, M., PRISTAS, P., CZIKKOVA, S., JAVORSKY, P.: A bacteriocin-mediated antagonism by Enterococcus faecium BC25 against ruminal Streptococcus bovis. Microbiol. Res., 153, 1998, 1-5. Cited 11 times

4. LAUKOVA, A. - MAREKOVA, M.-JAVORSKY, P: Detection and antimicrobial spectrum of a bacteriocin-like substance produced by Enterococcus faecium CCM4231. In Letters in Applied Microbiology, V16, no. 5, 1993, p.257-260. Cited 10 times

5. MOROVSKY, M. - PRISTAS, P. - JAVORSKY, P. Bacteriocins of ruminal bacteria. FOLIA MICROBIOLOGICA 46 (1): 61-62 2001 Cited 10 times

6. LAUKOVA, A.-MAREKOVA, M. Production of bacteriocins by different enterococcal isolates. In: Folia Microbiologica. Vol.46, no.1, 2001, p.49-52. Cited 10 times

7. LAUKOVA, A. - CZIKKOVA, S. The use of enterocin CCM4231 in soy milk to control the growth of Listeria monocytogenes and Staphylococcus aureus. In Journal of Applied Microbiology (1999), vol. 87, p. 182-186. Cited 9 times

8. KMET, V. – LUCCHINI, F.: Aggregation-promoting factor in human vaginal Lactobacillus strains. FEMS Immunol. Med. Microbiol. 19, 111-114, 1997 Cited 9 times

9. LAUKOVA A.- VLAEMYNCK G.-CZIKKOVA S. Effect of enterocin CCM4231 on Listeria monocytogenes in Saint-Paulin cheese. In: Folia Microbiologica. Vol. 46, no. 2, 2001, p. 157-160. Cited 8 times

10. JALC,D.- NERUD,F.- ZITNAN,R.- SIROKA,P.: The effect of white-rot basidomycetes on chemical composition and in vitro digestibility of wheat straw. In: Folia Microbiologica, 41(1),1996, 73-75 Cited 8 times

11. List of top-cited authors from the Organisation (at most 10 % of the research

employees) and their number of citations in the assessment period

[1] MVDr. Andrea Lauková , PhD – 118 citations

[2] Assoc. Prof. MVDr. Juraj Koppel, DrSc. – 68 citations

[3] Assoc. Prof. RNDr. Peter Javorský, DrSc. – 60 citations

12. Supplementary information and/or comments on responses to the scientific output of the Organisation

The scientific output of the Institute during assessed period (2003-2006) comprises 146 CC

publications. The significant proportion (76 from 146) of these papers was published in

veterinary (43 papers, field median - 0,53), agricultural (30 papers, field median – 0,788) and

food sciences (3 papers, field median – 0,708). This distribution corresponds well to the

18

framework of the Institute foundation charter but it explains also why the number of citations

is apparently low.

3. Research status of the Organisation in the international and national context

• International/European position of the Organisation i. List of the most important research activities documenting international

importance of the research performed by the Organisation, incl. major projects (details of projects should be supplied under Indicator 4). Collective membership in the international research organisations, in particular within the European Research Area

[4] Participation in FP5 5 RTD projects: QLK1-CT-1999-00226 Food PCR, QLRI-CT-

2000-01455 Ercule, QLK3-CT-2002-02151 CIMES, QLK1-CT-2002-02240

Tradisausage, QLK1-CT-2002-02240 Diet and Obesity

[5] Participation in FP5 CA - QLK2-CT-2001-01267 Genus Clostridium

[6] Associated partner in FP5 RTD project EVK2 – 2002 – 000538 Lake Baikal

ii. List of international conferences (co-) organised by the Organisation

[1] ISAM 2003, International Symposium on Anaerobic Microbiology, June 2003, Košice

[2] XXIst Conference on Animal Physiology, September 2004, Košice

iii. List of international journals edited/published by the Organisation

[1]

[2] …

iv. List of edited proceedings from international scientific conferences and other

proceedings

[1] Proceedings of the ISAM 2003, International Symposium on Anaerobic Microbiology,

June 2003, Košice

[2] Proceedings of the International scientific conference – XXIst Conference on Animal

Physiology, September 2004, Košice

19

• National position of the Organisation i. List of selected most important national projects (Centres of Excellence,

National Reference Laboratories, Agency for the Promotion of Research and Development (APVV/APVT), National Research Programmes, Scientific Grant Agency of the Slovak Academy of Sciences and the Ministry of Education

(VEGA), and others) [1] Principal investigators of 5 RTD APVT/APVV projects: APVT-51-012602 Microbial

genetic ecology of digestive tract, APVT-51-006204, Analysis of apoptotic processes

in preimplantation embryo, APVT-51-015404 Plant extracts - antiinflammatory,

immunomodulatory, cytotoxic and antimutagenic effects in animals, APVT-51-

007604 Environmental risks associated with DNA transfer from genetically modified

plants to microorganisms, APVT-51-004804 Effects of subtoxic concentrations of

mycotoxins in feed components produced in Slovak Republic on immunity and

antioxidant status of poultry

[2] Participations in 3 RTD APVT/APVV projects: APVT-51-036102 Advanced coal

materials on the base of Slovak raw materials for sustainable development, APVT-

51-006304 Modification of conditions for bacterial activity optimalization in processes

of non-metallic raw materials treatments, APVV-20-041605 The complex solving of

salmonelosis prevention with immunomodulators in poultry

[3] Principal investigator in one RTD project in the framework of State Program of

Science and Development SP 51/028 09 00/028 09 06 - Alternative ways of

antibiotic replacement – influencing of digestion physiology in animals

[4] Principal investigators in 9 VEGA projects

ii. List of national scientific conferences (co)-organised by the Organisation

[1] Scientific conference “New trends in veterinary physiology”, October 2006, Košice

[2] …

iii. List of national journals published by the Organisation

[1]

[2] …

20

iv. List of edited proceedings of national scientific conferences/events

[1] Fabian, D. Proceedings of Scientific conference “New trends in veterinary

physiology”, October 2006, Košice: Institute of Animal Physiology SAS, 2006. 75 p.

ISBN 80-968618-1-6.

[2] …

• International/European position of the individual researchers i. List of invited/keynote presentations at international conferences, documented

by an invitation letter or programme

[1] Pristas P.: How many rumen bacteria are there: lessons from Selenomonas

ruminantium. International Symposium on Anaerobic Microbiology, Jablonna near

Warsaw, Poland, September 2005. Opening plenary lecture.

[2] Lauková, A., Burdová, O., Strompfová, V., Marcinaková, M, Mareková, M. Kuzmová,

R. Bacteriocins, enterocins and their antimicrobial effect in dairy products. European

Dairy Congress-Milk and dairy products, Portorož, Slovenia, November, 2003. Invited

lecture.

ii. List of employees who served as members of the organising and/or programme committees for international conferences

[1] Javorsky P. International symposium on anaerobe microbiology ISAM-2005-

Warsaw, Poland

[2] Javorsky,P Structure and stability of proteins SSP-2003, Kosice, Slovakia;

[3] Javorsky,P Structure and stability of biomacromulecules SSB-2005, Kosice, Slovakia

iii. List of employees who served as members of important international scientific

bodies (e.g. boards, committees, editorial boards of scientific journals)

[1] Dr. J. Koppel – evaluator – rapporteur in evaluation panels for FP6 projects 2003-

2006

[2] Dr. L. Leng – member of Panel on additives and products or substances used in

animal feed [FEEDAP] – European Food Safety Authority

[3] Dr. L. Leng – member – rapporteur of Standing Working Group on “Trace Elements”

FEEDAP – European Food Safety Authority

21

[4] Dr. M. Baran – member of editorial boards - Electronic Journal of Agricultural Polish

Universities, Acta Scientiarum Polonarum

[5] Dr. A. Lauková – member of editorial board – International Journal of Probiotics and

Prebiotics

iv. List of international scientific awards and distinctions

[1]

[2] …

• National position of the individual researchers i. List of invited/keynote presentations at national conferences documented by

an invitation letter or programme

[1] Kmet V., Ohlasova D.: Metalobetalaktamazy ako pricina multirezistencie

Pseudomonas aeruginosa. Molekularna charakteristika a moznosti fenotypickej

identifikacie. VIII. Odborna konferencia sekcie klinickej mikrobiologie SLK,

Bobrovecka dolina 19-21.5. 2006.

ii. List of employees who served as members of organising and programme committees of national conferences

[1]

iii. List of employees serving in important national scientific bodies (e.g. boards, committees, editorial boards of scientific journals)

[1] Dr. Koppel - Member of Slovak Academy of Agricultural Sciences, Member of

Learned Society of Slovak Academy of Sciences, Member of Council of Slovak

Academy of Sciences for the Centres of Excellence, Member of the Council of

Agricultural and Forestry Sciences of Slovak Research and Development Agency

[2] Dr. Kmet – Member of Working group: biology of Council of Natural Sciences of

Slovak Research and Development Agency

[3] Dr. Koppel, Dr. Javorský – members of working groups of the Assessment

Commission of the Ministry of Education.

[4] Dr. Javorský – Head of East Slovakian branch of Slovak Society of Biochemistry

22

[5] Dr. Pristas, Dr. Lauková, Dr. Mozes – members of councils of VEGA agency

iv. List of national awards and distinctions

[1] Dr. J. Koppel – Honour Medal for the Development of Research in Biology – 2004,

Slovak Academy of Sciences

[2] Dr. P. Pristas, Dr. P. Javorsky – Award of Slovak Academy of Sciences for the

Research Results in 2005

Supplementary information and/or comments documenting international and national status of the Organisation

4. Project structure, research grants and other funding resources

• International projects and funding i. List of major projects within the European Research Area – 5th and 6th

Framework Programme of the EU, European Science Foundation, NATO, COST, INTAS, CERN, etc. (here and in items below please specify: type of project, title, grant number, duration, funding, responsible person in the Organisation and his/her status in the project, e.g. coordinator, principal investigator, investigator)

[1] FP5 RTD project QLK1-CT-1999-00226 Validation and standardization of diagnostic

Polymerase Chain Reaction for detection of foodborne pathogens (FOOD-PCR)

principal investigator: Doc. MVDr. Vladimir Kmet, DrSc., 1.3.2000 - 30.6.2003, EC -

60 720 Euro

[2] FP5 RTD project QLRI-CT-2000-01455 European rumen ciliate culture collection.

Principal investigator: Doc. RNDr. Peter Javorsky, DrSc. 1.9.2000 – 31.8.2003, EC –

89 520 Euro

[3] FP5 CA QLK2-CT-2001-01267 Pathology and Ecology of the Genus Clostridium in

Humans, Animals and Foodstuffs: Identification, Epidemiology and Prophylaxis.

Principal investigator: Doc. MVDr. Vladimir Kmet, DrSc. 1.10.2001 – 30.9.2004, EC

3 000 Euro

23

[4] FP5 RTD project QLK3-CT-2002-02151Ciliates as Monitors for Environmental Safety

of GMO. Principal investigator RNDr. Peter Pristas, PhD 1.10.2002 – 30.12.2005, EC

144 960 Euro

[5] FP5 RTD QLK1-CT-2002-02240 Assessment and improvement of safety of

traditional dry sausages from producers to consumers. Principal investigator: MVDr.

Andrea Laukova, PhD 1.1.2003 – 31.12.2005, EC – 100 800 Euro

[6] FP5 RTD QLK1-CT-2002-02240 Dietary and genetic influences on susceptibility or

resistance to weight gain on a high fat diet. Principal investigator: MVDr. Stefan

Mozes, PhD 15.2.2002 – 30.6.2004, EC – 54 000 Euro

[7] FP5 RTD EVK2 – 2002 – 000538 – Extension to High–resolution CONTINENTal

paleoclimate record in the Lake Baikal, subcontractor of the Institute of Geotechnics,

Kosice, investigator RNDr. Peter Pristas, PhD 21.7.2003 – 15.5.2004, EC -2 000

Euro

ii. List of other international projects incl. funding

[1] Project SMIAG - Rimonyx, Ltd., Izrael Small molecular inhibitors of adhesion on

glycosoaminoglycans. Coordinator MVDr. Juraj Koppel, DrSc.1.7.2002 – running, 5

millions SKK (2003-2006).

[2] Czech Republic - GAČR 524/06/0264 Ciliates of the genus Troglodytella: pathogens

or endosymbionts? A novel approach towards the veterinary care and understanding

the digestion in great apes. Investigator: RNDr. Svetlana Kišidayová, PhD, 2006-

2008. Grant Agency of Czech Republic. 60 000 SKK

[3] Italy - Molecular diagnostics of microbial interactions in the health gut. Principal

investigator: Prof. MVDr. Vladimir Kmeť, DrSc. 2004-2007, Ministry of Education of

Slovak Republic. 300 000 SKK

[4] Czech Republic - Effects of natural compounds on bacteria in animal digestive tract.

Principal investigator: Prof. MVDr. Vladimir Kmeť, DrSc. 2006-2007, APVV 72 000

SKK

[5] Czech Republic - Affecting of the production of polyunsaturated fatty acids – PUFA

and their isomers in rumen fluid and milk of ruminants with different diets and oilseed

supplements. Principal investigator: MVDr. Dusan Jalc, PhD, 2006-2007, APVV

72 000 SKK

[6] Slovenia - Selection of bakteriocinogenic and probiotic lactic acid bacteria from

traditional slovak and slovene cheeses with the aspect to improve their hygienic

24

quality. Principal investigator: MVDr. Andrea Laukova, PhD, 2006-2008, APVV

130 000 SKK

[7] Czech Republic - Role of bifidobacteria and lactobacili in poultry digestive tract.

Principal investigator: Assoc. Prof. MVDr. Vladimir Kmeť, DrSc. 2004-2005, Ministry

of Education of Slovak Republic 68 000 SKK

[8] Czech Republic - Molecular biology diagnostic of animals digestive tract microflora

focused on bifidobacteria. Principal investigator: Assoc. Prof. MVDr. Vladimir Kmeť,

DrSc. 2002-2003, Ministry of Education of Slovak Republic 26 000 SKK

[9] Czech Republic - Characterization of bacteriocin producing environmental

Enteroccoci and Staphyloccoci strains for their utilization for waste biological

decontamination. Principal investigator: MVDr. Andrea Laukova, PhD, 2002-2003,

Ministry of Education of Slovak Republic 26 000 SKK

iii. List of other important projects and collaborations without direct funding

[1] Effects of probiotics and prebiotics on Escherichia coli in conditions in vitro and in

vivo in rabbits. Institute of Animal Physiology and Genetic, Academy of Sciences of

Czech Republic, Prague. Principal investigator: MVDr. Andrea Laukova, PhD

[2] Influence of conjugated linoleic acid (cis 9, trans 11 C18:2, CLA) in cow milk and

trans-vaccenic acid (TVA) and CLA in rumen. Agricultural University, Poznan,

Poland. Principal investigator: MVDr. Dusan Jalc, PhD

[3] Role of key phosphatases and proteinkinases at enter of oocyte into maturation

stage during oogenesis. Institute of Animal Physiology and Genetic, Academy of

Sciences of Czech Republic, Libechov. Principal investigator: MVDr. Vladimir Baran,

PhD

[4] Characterization of lactic acid bacteria from the traditional fermented Portugal

saussage chourico. Faculty of Veterinary Medicine in Lisbon, Portugal. Principal

investigator: MVDr. Andrea Laukova, PhD

[5] ECONET- Mycotoxin impact on animal nutrition. INRA, Laboratory of Pharmacology

and Toxicology, Toulouse, France. Principal investigator: MVDr. Lubomir Leng, DrSc.

• National projects and funding i. List of projects supported by the Agency for the Promotion of Research and

Development (APVV/APVT), National Research Programmes, and their funding

25

[1] APVT-51-012602 Microbial genetic ecology of digestive tract, coordinator: Assoc.

prof. RNDr. Peter Javorsky, DrSc.: 2002-2005, APVT 2,328 mil. SKK

[2] APVT-51-006204, Analysis of apoptotic processes in preimplantation embryo,

coordinator: MVDr. Dusan Fabian, PhD, 2005-2007, APVT 2 mil. SKK

[3] APVT-51-015404 Plant extracts - antiinflammatory, immunomodulatory, cytotoxic and

antimutagenic effects in animals, coordinator: Assoc. prof. MVDr. Juraj Koppel,

DrSc., 2005-2007, APVT 5,536 mil. SKK

[4] APVT-51- 007604 Environmental risks associated with DNA transfer from genetically

modified plants to microorganisms, coordinator: Assoc. prof. RNDr. Peter Javorsky,

DrSc, 2005-2007, APVT 2,85 mil. SKK

[5] APVT-51-004804 Effects of subtoxic concentrations of mycotoxins in feed

components produced in Slovak Republic on immunity and antioxidant status of

poultry, coordinator: MVDr. Lubomir Leng, DrSc., 2005-2007, APVT 3,529 mil. SKK

[6] APVT-51-036102 Advanced coal materials on the base of Slovak raw materials for

sustainable development, investigator: MVDr. Andrea Laukova, 2004-2005, APVT

0,23 mil. SKK

[7] APVT-51-006304 Modification of conditions for bacterial activity optimalization in

processes of non-metallic raw materials treatments, investigator: MVDr. Igor Styriak,

2005-2007, APVT 0,1 mil. SKK

[8] APVV-20-041605 The complex solving of salmonelosis prevention with

immunomodulators in poultry, investigator: MVDr. Andrea Laukova, 2006-2008,

APVV 0,18 mil. SKK

[9] RTD project in the framework of State Program of Science and Development SP

51/028 09 00/028 09 06 - Alternative ways of antibiotic replacement – influencing of

digestion physiology in animals, coordinator: RNDr. Dobroslava Bujnakova, PhD,

2003-2005, SAS 4,843 mil. SKK

ii. Number of projects supported by the Scientific Grant Agency of the Slovak Academy of Sciences and the Ministry of Education (VEGA) for each year, and their funding

VEGA 2003 2004 2005 2006

number 9 8 10 9

funding (millions of SKK) 1,237 1,332 1,352 1,386

26

• Summary of funding from external resources

External resources 2003 2004 2005 2006 total average

external resources (millions of SKK) 10,314 6,299 12,309 11,967 40,889 10,222

external resources transfered to coooperating research organisations (millions of SKK)

0,000 0,000 0,233 0,435 0,668 0,167

ratio between external resources and total salary budget 1,110 0,685 1,284 1,160 4,239 1,060

overall expenditures (millions of SKK) 27,458 25,180 29,490 29,346 111,474 27,869

Supplementary information and/or comments on research projects and funding resources

5. Organisation of PhD studies, other pedagogical activities i. List of accredited programmes of doctoral studies (as stipulated in the

previously effective legislation as well as in the recently amended Act on the Universities)

Previous legislation - 3: Veterinary Physiology, Biochemistry, Animal Physiology Recent legislation – 4: Veterinary Anatomy and Physiology, Biochemistry, Animal Physiology, Microbiology

ii. Summary table on doctoral studies (number of internal/external PhD students; number of students who completed their study by a successful thesis defence; number of PhD students who quitted the programme)

27

PhD study

number of potential PhD supervisors

PhD students

num

ber

defe

nded

thes

is

stud

ents

qui

tted

num

ber

defe

nded

thes

is

stud

ents

qui

tted

num

ber

defe

nded

thes

is

stud

ents

qui

tted

num

ber

defe

nded

thes

is

stud

ents

qui

tted

internal 16 1 2 13 2 14 3 10 4 1

external 4 1 2 2 1 3

supervised at external institution by the research employees of the assessed organisation

1 1 1 1 1

16 16 16 16

31.12.200531.12.2003 31.12.2004 31.12.2006

iii. Postdoctoral positions supported by a) external funding (specify the source)

2004 – 2005 EU FP5 project CIMES – RNDr. Mária Piknová, PhD

2006-2007 – RNDr. Mária Piknová, PhD, Dalhousie University, Halifax (Canada),

funded by Dalhousie University

2005-2007 – RNDr. Adriana Šprincová, PhD, Dubna (Russia) – funded by Ministry of

Education, Slovak Republic

b) internal funding - the Slovak Academy of Sciences Supporting Fund of Stefan

Schwarz

2003 – RNDr. Dobroslava Bujňáková, PhD

2005 – MVDr. Viola Strompfová, PhD

2006 – RNDr. Mária Piknová, PhD

iv. Summary table on pedagogical activities in undergraduate programmes for each year

28

Teaching 2003 2004 2005 2006

lectures (hours/year) 123 101 84 114

practicum courses (hours/year) 256 283 72 96

supervised diploma works (in total) 10 6 7 12

members in PhD committees (in total) 5 5 5 7

members in DrSc. committees (in total) 3 4 4 3

members in university/faculty councils (in total) 2 1 1 1

members in habilitation/inauguration committees (in total) 3 3 3 6

v. List of published university textbooks

[1] ANGELOVICOVA, M. - NIKOLAJCUK, V. I. - TURIANICA, I., KMET, V. - MASSANYI,

P. Dietetika a hygiena krmiv. Uzhorod: vyd. V.Paďaka, 2005, 224 s., ISBN 966-7838-

78-1.

[2] FAIX, S. Peripheral circulatory failure. In: FAIXOVA, Z. (Ed.), General veterinary

pathophysiology, University of Veterinary Medicine, Kosice, 2005, p. 43-57 ISBN 80-

8077-018-2.

[3] FAIX, S. Fever. In: Faixova, Z. (Ed.), General veterinary pathophysiology, University

of Veterinary Medicine, Kosice, 2005, p. 150-154 ISBN 80-8077-018-2.

[4] TANCINOVA, D.- MAKOVA, J. - FELSÖCIOVA, S. - KACANIOVA, M. - KMET, V.

Mikrobiologia potravin. Nitra: SPU, 2005 150 s. ISBN 80-8069-568-7.

[5] MARACEK, I – HALAGAN, J. – STANIKOVA, A. – PASTOROVA, B. – SOPKOVA, D.

– KOPPEL, J., - KOSTECKA, Z. – VLCKOVA, R. Basis of Physiology for Cynologists.

University of Veterinary Medicine, Kosice, 2006, p. 222 p. ISBN 80-8077-046-8

vi. Number of published academic course books

vii. List of joint research laboratories/facilities with the universities

[1]

29

[2] ...

viii. Supplementary information and/or comments on doctoral studies and pedagogical activities

6. Direct output to the society (applications of results, popularisation and outreach activities) i. List of the most important results of applied research projects

[1] Development of functional food – eggs enriched with the natural form of selenium

(collaboration with Novogal a.s. and PG Trade)

[2] Real time PCR method for detection and quantification of M. paratuberculosis IS 900

gene in cow’s faeces (collaboration with State Veterinary and Food Institute Nitra)

[3] VET MIC Kit for the investigation of minimal inhibitory concentrations in gramnegative

animal enterobacteria (performed by Miditech Bratislava)

[4] New starter culture for fermented meat products with protective effects (MasoSpis,

subdivision Kosice).

ii. List of the most important studies commissioned for the decision-making authorities, the government and NGOs, international and foreign organisations

[1]

[2] …

iii. List of the most important popularisation activities

[1]

[2] …

iv. List of patents issued abroad, incl. revenues

[1]

[2] …

30

v. List of the patents issued in Slovakia, incl. revenues

[1]

[2] …

vi. List of licences sold abroad, incl. revenues

[1]

[2] …

vii. List of licences sold in Slovakia, incl. revenues

[1]

[2] …

viii. List of contracts with industrial partners, incl. revenues

[1]

[2] …

ix. List of research projects with industrial partners, incl. revenues

[1] Project SMIAG- Small molecular inhibitors of adhesion on glycosoaminoglycans

(collaboration with Rimonyx Pharmaceuticals Ltd., Rehovot, Israel) - the development

of new antiinflammatory compounds. (2003-2006 5 millions SKK)

[2] …

x. Summary of outreach activities

31

Outreach activities 2003 2004 2005 2006 total

studies for the decision sphere, government and NGOs, international and foreign organisations 0

articles in press media/internet popularising results of science, in particular those achieved by the Organization 5 3 3 1 12

appearances in telecommunication media popularising results of science, in particular those achieved by the Organization

1 5 4 10

public popularisation lectures 1 1

xi. Supplementary information and/or comments on applications and popularisation activities

Each year the Institute is organizing “The day of open doors” focused on students of

high schools and university students.

7. Background and management. Staffing policy and implementation of findings from previous assessments

i. Summary table of personnel Personnel 2003 2004 2005 2006

all personel 46 47 47 43

research employees from Tab. Research staff 28 30 30 27

FTE from Tab. Research staff 26,09 27,22 27,88 26,18

averaged age of research employees with university degree 47,8 47,08 45,18 46,18

ii. Professional qualification structure

32

Number of 2003 2004 2005 2006

DrSc. 6 6 6 6

PhD / CSc. 17 17 23 22

Prof. 1 1

Doc./Assoc. Prof. 3 3 2 4

iii. Status and development of research infrastructure incl. experimental, computing and technical base (description of the present infrastructure, premises, and material and technical resources. Infrastructure, instrumentation and major technical equipment necessary for the achievement of the objectives specified in the research Concept)

The research infrastructure of the Institute corresponds to the budget available in recent years.

Generally speaking it is appropriate mainly at the level of small and medium laboratory

equipment (including bench centrifuges, pH meters, PCR and RT PCR, equipment for

molecular biology, spectrophotometers, small microscopes, Perkin-Elmer Clarus 500 gas

chromatograph). However the Institute has certain disadvantages at the level of large and

expensive equipment (e.g. microarray, confocal microscope, spectrofluorometer), which are

either obsolete or unavailable. This situation should be radically corrected due to foreseen

financing from structural funds for research institutions in the coming years. Furthermore

these funds should help to reshape laboratories and animal facilities in accordance with recent

legislation and modern research trends.

iv. Status and development of bibliographic resources, activities of the Organisation’s library and/or information centre

The library of the Institute comprises 2660 resource items. Nowadays the main source of

research information is based on available internet databases (ScienceDirect, Web of Science,

Scopus and others administered by the Central Library of SAS, Bratislava), which are fully

available to all researchers and PhD students. Computers with high-speed access to the

internet are provided in all Institute premises for all researchers and students.

33

v. Describe how the results and suggestions of the previous assessment were taken into account

The last “Evaluation report” recommended the introduction of molecular biology methods in

each research branch. Evaluators also recommended the procurement of new equipment, and

to improve the age composition of researchers at the Institute.

Since the last evaluation the Institute has purchased Real Time PCR equipment Mx 3000P

(Stratagene) as well as PCR cyclers. The use of molecular biology methods has increased

considerably in almost all research groups. During the last four years some finishing PhD

students have been employed in postdoctoral positions at the Institute, thus improving the age

profile and ensuring a promising balance among the research staff.

vi. Supplementary information and/or comments on management, research infrastructure, and trends in personnel development

The Institute strictly adheres to the principles of Good Scientific Practice (published

by the Science and Technology Assistance Agency) as well as to the national legislation

relevant to animal welfare and ethics of animal research. The Institute has all necessary

authorizations for research in restricted areas, e.g. animal research, GMO, infectious agents

and hazardous chemical and biological substances.

The Institute has a long-term plan for human resources management based on the

inevitably important age balance of research personnel, and has adopted an optimal strategy

for increasing the proportion of PhD students and young postdoctoral scientists. These efforts

are limited only by budgetary restrictions, and moreover they have been partly overcome

through our active search for other financial resources (see above).

All researchers are regularly evaluated at yearly intervals and the evaluation results are

reflected also in the variable part of their remuneration. This individual evaluation

(established more than 15 years ago) is based mainly on the quality of CC publications

(assessed on similar principles as the current assessment of the Institute) as well as their

involvement in PhD students mentoring and teaching of university students.

The organizational structure of the Institute is flexible on principle, based on the

financed research projects. The research groups cooperate intensively together as well as with

the other research institutions in Slovakia and abroad, and their high level of fruitful

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collaboration is also reflected in the list of publications. The active policy of the Institute

management is based on universal support focused mainly on international projects and large

national projects in all stages, starting at the proposal stage, through negotiation of contract,

implementation and execution of the project, management of financial aspects, assistance with

reports, intellectual property, copyrights, patents, licenses, and technology transfer. All major

research projects are consulted and approved by the Research Council of the Institute. All

project leaders periodically (at least once per year) present and discuss their results with other

researchers and PhD students in the framework of the scientific seminars. Every month the

PhD students have an informal meeting with the head of the Institute, where they present their

achievements and progress in their study. Every year the PhD students present their results as

part of the PhD seminar. Starting in 2006 this seminar was integrated into the research

workshop “New trends in veterinary physiology”, organized jointly with the University of

Veterinary Medicine in Kosice.

The head of the Institute is in permanent contact with the project leaders, and

exchange is based not only on formal/informal meetings but also on electronic

communications (email, phone) because the Institute is based at two separated locations in

Kosice. Broadband internet connection is assured in both locations, including VoIP facilities.

Other information relevant to the assessment