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RCPAQAP Molecular Infectious DiseasesGeneric ReportMAF1:2015Molecular Alphavirus / Flavivirus

CopyrightThis material is copyright and may not be used in any form for advertising, sales promotion or publicity. The material maynot be reproduced in whole or in part for any purpose whatsoever (including presentations at meetings and conferences),without the prior written permission of the RCPA Quality Assurance Programs Pty Limited. Permission must be sought inwriting from the Program but will not be unreasonably refused.

ConfidentialityRCPA Quality Assurance Programs Pty Limited keeps all participant details confidential. No information related to any of theparticipants will be divulged to a third party, unless required by legislation, without the express written consent of theparticipant. General information may be discussed at meetings or presented as papers to journals.

RCPA Quality Phone +61 2 9045 6000Assurance Programs Fax +61 2 9356 2003

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© 2015 RCPA Quality Assurance Programs Pty Ltd. All rights reservedReport Issued Monday, 18 May 2015Page of 28

Generic Report MAF1:2015Participant ResultsThe Molecular Alphavirus/Flavivirus Program was distributed to 14 participants.12 participants returned results for this survey.

Clerical ErrorThere was a clerical error included in this survey for Specimen 1B.

Specimen 1B labelled: Rachael SCHULT; U/N 257032Instructions to Participant labelled: Rachael SCHULTZ: U/N 257032

9/12 (75%) participants detected the clerical error for Specimen 1B.

3/12 (25%) participants (1187, 83000 and 89082) did not identify the clerical error.

Specimen DetailsSamples consist of inactivated virus (gamma inactivation radiation (50 kGy)) from human strains of alphavirus orflavivirus suspended in 250uL of tissue culture fluid or molecular grade water.(Ref. 2015 RCPAQAP Molecular Infectious Diseases Program Plan section MAF1:2015)

1A Positive for Alphavirus, Ross River virus.

1B Negative, to assess contamination.

1C Positive for Flavivirus, Dengue 3 virus.

1D Positive for Alphavirus, Chikungunya virus.

1E Positive for Flavivirus, Dengue 1 virus.

1F Positive for Flavivirus, Japanese Encephalitis.

Source: Molecular Diagnostics & Development, Division of Microbiology and Infectious Diseases, PathWest LaboratoryMedicine, WA and RCPAQAP Serology MDM Serum Bank.

Preliminary & Pre-issue testing: Molecular Diagnostics & Development, Division of Microbiology and Infectious Diseases,PathWest Laboratory Medicine, WA and Public Health Laboratory, Queensland Health Forensic and Scientific Services,QLD.

Survey AimsThe detection and identification of Alphavirus (Ross River and Chikungunya) and Flavivirus (Dengue type 1, Dengue type3 and Japanese Encephalitis). A negative is included to assess contamination.


Clinical Notes (written by Dr Ed Raby)The “Clinical Notes” were created so as to highlight particular issues relating to interpretation of results. They do notnecessarily reflect the clinical history of the patient from whom the sample was obtained.

1A 24 year old female with fever, joint pains, rash. Rubella?

1B 42 year old female with polyarthralgia, tired.

1C 18 year old male, headache, rash, returned from Fiji.

1D 64 year old male, fever, hand and ankle pains, recent travel to Bali.

1E 32 year old male, travel to Thailand, fever, thrombocytopaenia.

1F 20 year old female, holiday work on pig farm in Vietnam, polio‐like syndrome.

Pre-issue ResultsThese results were obtained in pre-issue testing of the survey specimens.

Specimen Alphavirus Result Flavivirus Result Virus Identification[RT PCR]

1A positive negative Ross River

1B negative negative not applicable

1C negative positive Dengue 3

1D positive negative Chikungunya

1E negative positive Dengue 1

1F negative positive Japanese Encephalitis

Method PerformanceIt is up to all users to form their own opinion or conclusions on each kit's performance based on the accumulation ofdata and information that is supplied by this survey, and other information available to the participant.

Please note when primer sequence information is returned by participants it is not published to protect confidentiality.


Result Review

ALPHAVIRUS Results10 participants returned results for Alphavirus molecular analysis.

Specimen 1A is positive for an Alphavirus; Ross River virus

3/4 participants (10003, 40002 and 83000) correctly detected an Alphavirus using Alphavirus group testing.Participant 10003 further commented on the identification of Ross River virus.

Participant 89112 failed to detect an Alphavirus using specific Alphavirus group testing.

All participants (40002, 60003 and 80071) performing specific Ross River virus testing identified the Ross River virus.

Specimen 1B is negative for an Alphavirus; all participants correctly returned a negative / not detected result.

Specimen 1C is negative for an Alphavirus; all participants correctly returned a negative / not detected result.

Specimen 1D is positive for an Alphavirus; Chikungunya virus.

3/4 participants (10003, 40002 and 83000) correctly detected an Alphavirus using Alphavirus group testing.Participants 10003 and 83000 further commented on the identification of Chikungunya virus.

Participant 89112 failed to detect an Alphavirus using specific Alphavirus group testing.

All participants (10003, 40002, 60003, 80011, 80042, 80069, 80071, 83000, 89082 and 89112) performing specificChikungunya virus testing identified the Chikungunya virus.

Specimen 1E is negative for an Alphavirus; all participants correctly returned a negative / not detected result.

Specimen 1F is negative for an Alphavirus; all participants correctly returned a negative / not detected result.

The results reported by Participant 1187 in the Alphavirus section were moved to the Dengue section as a data entryerror had been made.

Table 1 Alphavirus Group5 participants returned results.

Molecular A/F Alphavirus Group

Part1A 1B 1C 1D 1E 1F

Result Comment Result Comment Result Result Comment Result Result

10003 detected typed as ross rivervirus by sequencing

notdetected

notdetected detected

typed as chikungunyavirus by real-time PCR(Ct=20) & confirmed bysequencing

notdetected

notdetected

40002 detected notdetected

notdetected detected not

detectednot

detected

83000 detected A type of Alphavirusdetected

notdetected - not

detected detected

A type of Alphavirusdetected, typed asChikungunya by real-time RT-PCR

notdetected

notdetected

89112 negThis result does notexclude a recentAlpha virus infection

negThis result does notexclude a recentAlpha virus infection

nottested neg

This result does notexclude a recent Alphavirus infection

nottested

nottested

Participant 89112 appears to have used an expired amplification kit.

Participants 40002 and 89112 have not provided inhibition control information.


ALPHAVIRUS Results (continued)

Table 2 Barmah Forest Virus2 participants returned results.

Molecular A/F Barmah Forest Virus

Part 1A 1B 1C 1D 1E 1F

40002 not detected not detected not tested not detected not tested not tested

60003 not detected not detected not detected not detected not detected not detected

Participant 40002 has not provided inhibition control information.

Table 3 Chikungunya Virus10 participants returned results.

Molecular A/F Chikungunya Virus


10003 not detected not detected not detected detected not detected not detected

40002 not detected not detected not tested detected not tested not tested





80071 neg neg neg pos neg neg


89082 neg neg neg pos neg neg

89112 neg neg not tested pos not tested not tested

Participant 80069 commented ‘Chikungunya not detected’ for Specimens 1A, 1B, 1C, 1E and 1F.

Participant 89112 commented ‘This result does not exclude a recent Chikungunya virus infection’ for Specimens 1A and1B. Participant 89112 commented ‘This result suggest a recent infection with Chikungunya virus’ for Specimen 1D.

Participants 40002, 80069, 80071, 89082 and 89112 have not provided inhibition control information.

Table 4 Ross River Virus3 participants returned results.

Molecular A/F Ross River Virus


40002 detected not detected not tested not detected not tested not tested

60003 detected not detected not detected not detected not detected not detected

80071 pos neg neg neg neg neg



ALPHAVIRUS Results (continued)

Table 5 Sindbis Virus1 participant returned results.

Molecular A/F Sindbis Virus


40002 not detected not detected not tested not detected not tested not tested


FLAVIVIRUS Results12 participants returned results for Flavivirus molecular analysis.

Specimen 1A is negative for a Flavivirus; all participants correctly returned a negative / not detected result.

Specimen 1B is negative for a Flavivirus; most participants correctly returned a negative / not detected result.

Participant 10003 incorrectly detected a Flavivirus using Flavivirus group testing and commented ‘typed as japaneseencephalitis virus by sequencing’. This participant reported a correct result of ‘not detected’ for specific JapaneseEncephalitis virus, however commented ‘positive in Flavivirus Group assay and typed as JEV by sequencing’.

Specimen 1C is positive for a Flavivirus; Dengue 3 virus.

All participants correctly detected a Flavivirus using Flavivirus group testing.Participants 10003 and 83000 also correctly commented on the identification of Dengue virus 3.

All participants performing specific Dengue virus testing identified the Dengue virus.Participants 10003, 40002, 60003, 80042, 80069, 80071 and 83000 also correctly identified the virus as Dengue type 3.

Specimen 1D is negative for a Flavivirus; most participants correctly returned a negative / not detected result.

Participant 10003 incorrectly detected a Flavivirus using Flavivirus group testing and commented ‘typed as dengue virustype 3 by real-time PCR (Ct=36) & confirmed by sequencing’. This participant also incorrectly identified Dengue type 3,using specific Dengue testing.

Specimen 1E is positive for a Flavivirus; Dengue 1 virus.

All participants correctly detected a Flavivirus using Flavivirus group testing.Participants 10003 and 83000 also correctly commented on the identification of Dengue virus 1.

All participants performing specific Dengue virus testing identified the Dengue virus.Participants 10003, 40002, 60003, 80042, 80069, 80071 and 83000 also correctly identified the virus as Dengue type 1.

Specimen 1F is positive for a Flavivirus; Japanese Encephalitis virus.

All participants correctly detected a Flavivirus using Flavivirus group testing.Participants 10003 and 83000 also correctly commented on the identification of Japanese Encephalitis virus.

All participants performing specific Japanese Encephalitis virus testing identified the Japanese Encephalitis virus.

Participant 60003 incorrectly reported ‘detected’ for this specimen using specific West Nile Virus assay, furthercommenting ‘WN and JE POS’.


FLAVIVIRUS Results (continued)

Table 6 Flavivirus Group5 participants returned results.

Molecular A/F Flavivirus Group


Result Result Result Comment Result Result Comment Result Comment

10003 notdetected detected detected

typed as dengue virustype 3 by real-timePCR (Ct=17) &confirmed bysequencing

detected detected

typed as dengue virustype 1 by real-timePCR (Ct=18) &confirmed bysequencing

detected

typed as japaneseencephalitis virus byreal-time PCR (Ct=27)& confirmed bysequencing

40002 notdetected


detected detected detected

80071 neg neg pos neg pos pos

83000 notdetected


A type of Flavivirusdetected, typed asDengue 3 by real-timeRT-PCR


A type of Flavivirusdetected, typed asDengue 1 by real-timeRT-PCR

detectedA type of Flavivirusdetected, typed as JEby real-time RT-PCR

89112 neg neg posThis result suggest arecent infection withFlavivirus

neg posThis result suggest arecent infection withFlavivirus

posThis result suggest arecent infection withFlavivirus

Participant 10003 incorrectly returned ‘detected’ for Specimens 1B and 1D and further commented ‘typed as japaneseencephalitis virus by sequencing’ and ‘typed as dengue virus type 3 by real-time PCR (Ct=36) & confirmed by sequencing’respectively.

Participant 89112 commented ‘This result does not exclude a recent Flavivirus infection’ for Specimens 1A, 1B and 1D.

Participants 40002, 80071 and 89112 have not provided inhibition control information.

Table 7 Dengue Virus12 participants returned results.

Molecular A/F Dengue Virus


Result Result Result Comment Result Result Comment Result

1187 notdetected


detected detected notdetected

10003 notdetected

notdetected detected dengue virus type 3 detected detected detected dengue virus type 1

detectednot

detected

40002 nottested

notdetected detected Dengue 3 RNA was detected in

this samplenot

tested detected Dengue 1 RNA was detectedin this sample

notdetected

60003 notdetected

notdetected detected Dengue Type 3 POS not

detected detected Dengue Type 1 POS notdetected

80011 notdetected


detected detected notdetected

80042 notdetected

notdetected detected Dengue Type 3 not

detected detected Dengue Type 1 notdetected

80052 neg neg pos neg pos neg

80069 notdetected

notdetected detected DEN 3 Detected not

detected detected DEN 1 Detected notdetected

80071 neg neg pos D3 neg pos D1 neg

83000 notdetected

notdetected detected Dengue type 3 detected not

detected detected Dengue type 1 detected notdetected

89082 neg neg pos neg pos neg

89112 nottested

nottested pos This result suggest a recent

infection with Dengue virusnot

tested pos This result suggest a recentinfection with Dengue virus neg



Participant 1187 had their results moved from the Alphavirus section to the Dengue section based on the kit theyreported using.

Participant 10003 incorrectly returned ‘detected’ for Specimen 1D and further commented ‘dengue virus type 3detected’.

Participants 1187, 40002, 80042, 80069, 80071 and 89082 have not provided inhibition control information.

Participant 1187 has not provided negative or positive control information.

Participant 89112 appears to have used an expired amplification and detection kit.

Table 8 Japanese Encephalitis Virus7 participants returned results.

Molecular A/F Japanese Encephalitis Virus


Result Result Result Result Result Result Comment

10003 not detected not detected not detected not detected not detected detected

40002 not tested not detected not detected not tested not detected detected


80069 not detected not detected not tested not tested not tested detected Japanese Encephalitisvirus Detected

80071 neg neg neg neg neg pos

83000 not detected not detected not detected not detected not detected detected JE Virus detected

89082 neg neg neg neg neg pos

Participant 10003 has incorrectly commented ‘positive in Flavivirus Group assay and typed as JEV by sequencing’ forSpecimen 1B.

Participant 60003 has not provided master/sample volume for the assay in use.

Participants 40002, 80069, 80071 and 89082 have not provided inhibition control information.

Table 9 Kunjin Virus2 participants returned results.

Molecular A/F Kunjin Virus


40002 not tested not detected not detected not tested not detected not detected





Table 10 Murray Valley Encephalitis Virus3 participants returned results.

Molecular A/F Murray Valley Encephalitis Virus




80069 not detected not detected not tested not tested not tested not detected


Table 11 West Nile Virus5 participants returned results.

Molecular A/F West Nile Virus





89082 neg neg neg neg neg neg


Participant 60003 incorrectly returned ‘detected’ for Specimen 1F further commented ‘WN and JE POS’.

Participant 60003 has not provided master/sample volumes for the assay in use.

Participants 40002, 80069, 89082 and 89112 have not provided inhibition control information.

Participant 40002 has not provided negative control information.

Table 12 Yellow Fever Virus5 participants returned results.

Molecular A/F Yellow Fever Virus







Participants 40002, 80069, 80071, 89082 and 89112 have not provided inhibition control information.


Review

Clinical Notes [Discussion by Dr Ed Raby, PathWest, WA]The “Clinical Notes” were created so as to highlight particular issues relating to interpretation of results. They do notnecessarily reflect the clinical history of the patient from whom the sample was obtained.

1A 24 year old female with fever, joint pains, rash. Rubella?Typical symptoms of RRV include a rash in about 50% of patients that may be clinically indistinguishable fromthe rash of rubella.

1B 42 year old female with polyarthralgia, tired.

1C 18 year old male, headache, rash, returned from Fiji.Fiji has had more than 25,000 suspected cases of Dengue with 15 deaths and remains an important source ofimported cases to Queensland.

1D 64 year old male, fever, hand and ankle pains, recent travel to Bali.Chikungunya is now endemic in Bali and may be difficult to distinguish clinically from Dengue Fever. The jointpain/arthralgia of CHIK infection typically affects the distal joints, similar to RRV/BFV and in contrast to Denguewhich is typically more central.

1E 32 year old male, travel to Thailand, fever, thrombocytopaenia.Low platelet count (thrombocytopaenia) is a common finding in Dengue Fever although significant haemorrhageremains very rare in adult travellers.

1F 20 year old female, holiday work on pig farm in Vietnam, polio‐like syndrome.Japanese Encephalitis Virus is present throughout Asia and causes epidemics and seasonal disease. It is rare intravellers without prolonged rural exposure. Polio‐like flaccid paralysis as well as more generalised encephalitisoccur in a minority of patients with most infections remaining asymptomatic.

AlphavirusSpecimens 1A and 1D were positive for an Alphavirus.

Four participants submitted group results. Three participants correctly detected the presence of an alphavirus in boththe above specimens. Participant 89112 failed to detected alphavirus in either Specimen 1A or 1D despite successfuldetection of both Ross River virus and Chikungunya virus in previous surveys using a pan-alpha PCR.

Three participants returning alphavirus results identified Ross River virus in Specimen 1A using a specific PCR.

Ten participants returning alphavirus results identified Chikungunya virus in Specimen 1D using a specific PCR.

There were no false positive results.

FlavivirusSpecimens 1C, 1E and 1F were positive for a Flavivirus.

Five participants submitted group results. All participants correctly detected the presence of a flavivirus in the abovespecimens. False positive results reported in Specimens 1B and 1D should be further investigated by Participant 10003.This may represent clerical error, however appears most likely to be due to cross-contamination of specimens duringprocessing.

Twelve participants returned Dengue virus specific assay results and although all were correctly detected for Specimens1C and 1E, only seven were able to go on and provide typing results.


Japanese encephalitis virus (Specimens 1F) was detected by all seven participants using a specific PCR. The mixeddetection reported by Participant 60003 (West Nile virus) has been reported in previous surveys as ‘cross reactivity’ andmost likely relates to primer specificity.

General CommentsIt is noted that the terminology used by several participants is incorrect, inconsistent and/or incomplete. A standardisedapproach to data returned to the RCPAQAP is encouraged. Generally in-house manufactured controls, amplification anddetection systems are poorly defined. Data entry errors are also noted. The general use of a Ct value of 0 to define anegative is not informative and is technically incorrect. Some indication of the upper and lower thresholds for each assaywould be more useful information. i.e. NEG = Ct >45 ; POS = Ct <35.

Participant 60003 reported using 160uL of specimen in the QIAamp Viral RNA Mini Kit. This is a non-standard specimenvolume for this purification method. The same participant reported the use of a 12uL volume of nucleic acid with 8uL ofreaction mix. These results are contradictory to previous submissions and may be clerical errors.

Participant 89112 used an expired Dengue virus kit. Although this did not seem to affect detection of these surveyspecimens, it may not perform as efficiently on specimens nearer to the lower limit of detection of the assay.

All participants are encouraged to incorporate an inhibition control into each assay and consider the development of apan-alpha / pan-flavi approach where possible.

There has been a large increase in the number of RRV cases reported to the Centre for Communicable DiseaseIntelligence from both NSW and QLD in the year to date and Dengue virus detections in returned traveller’s remainshigh. The use of nucleic acid amplification detection techniques in the detection of arboviruses should be limited to thetransient acute phase of infection and typing for epidemiology. All molecular results should be considered in conjunctionwith IgM and IgG serological results

Comments prepared for and on behalf of RCPAQAP by Ms Joanne Montgomery, PathWest Laboratory Medicine,Western Australia.


Alphavirus Controls & In house Methods for Survey MAF1:2015:1A, 1B, 1C, 1D, 1E & 1F

Alphavirus Group - Controls & In house Methods

PartInhib Control Neg Control Pos Control In-house Methods

Manufacturer Lot No. Expiry Manufacturer Lot No. Expiry Manufacturer Lot No. Expiry In-house Method Detection Method

10003 In house - BVDVspiked into sample NFW Chikungunya virus cDNA

heminestedAlphavirus-genericassay

gel electrophoresis

40002 N/A In House NA In House synthetic 26022015 NA

83000 In-house Non Template Control Positive ChikungunyaIsolate

Selected humangene as inhibitorcontrol for PCR

89112 - - NA Promega (nucleasefree water) 14748722 Jan

2016In house (ChikungunyaRNA as Positive Control) NA NA Conventional Nested

PCRAgarose gelelectrophoresis and

Barmah Forest Virus - Controls & In house Methods


Manufacturer Manufacturer Expiry Manufacturer Lot No. Expiry In-house Method Detection Method

40002 N/A in house NA Primer control, Probe control 05022015,05022015 NA Taqman RT PCR Probe fluorescence

60003 In-house In-house In-house Yes Realtime PCR

Sindbis Virus - Controls & In house Methods

Part

Inhib Control Neg Control Pos Control In-house Methods


40002 N/A in house NA Primer Control, Probe Control 21052013,21052013 NA Taqman RT-PCR Probe Fluorescence


Alphavirus Controls & In house Methods for Survey MAF1:2015:1A, 1B, 1C, 1D, 1E & 1F (continued)

Chikungunya Virus - Controls & In house Methods



10003 In house - BVDVspiked into sample NFW Chikungunya virus cDNA real-time Chikungunya

specific TaqMan assay Real-time PCR

40002 N/A in house NA Primer Control, ProbeControl

23122014 ,05022015 NA Taqman RT-PCR Probe Fluorescence

60003 In-house In-house Reference Strain Yes Realtime PCR80011 In-house NA NA In-house NA NA In-house NA NA

80042 In-house In-house In-house Adapted frompublished method Real-time PCR

80069 NA NA NA In-house NA NA In-house NA NA - -

80071 NA NA NA Water NA NAIn house (CHIKV celllysate RNA as PositiveControl)

NA NA Adapted from Hasebeet al., 2002

LightCycler RNA MasterSYBR Green I

83000 In-house - NA Non TemplateControl - NA Positive Chikungunya

Isolate - NASelected human geneas inhibitor control forPCR

-

89082 In house Mar 15 Mar 16 In house Mar 15 Mar 16

89112 NA Promega (nucleasefree water) 14748722 Jan 2016 In house (CHIK RNA as

Positive Control NA NA Taqman RT-PCR LightCycler

Fluorescence/LightCycler

Ross River Virus - Controls & In house Methods



40002 N/A in house NA Primer control, Probe Control 10032015,10032015 NA Taqman RT-PCR Probe Fluorescence

60003 In-house In-house Reference strain Yes Realtime PCR

80071 NA NA NA Water NA NA In house (Ross river virus celllysate RNA as Positive Control) NA NA Adapted from

Studdert et al., 2003LightCycler® RNAMaster SYBR Green I


Alphavirus Units and Cut-Off Values for Survey MAF1:2015:1A, 1B, 1C, 1D, 1E & 1F

Part UnitsAlphavirus Group - Units & Cut Off

Neg Equ Pos

10003 PCR product size no specific electrophoresis sizeband/IC OK

specific electrophoresis sizeband/IC OK

40002 band intesity83000 MELT CURVE NO MELT CURVE - MELT CURVE DETECTED89112 No units available

Part Units

Barmah Forest Virus - Units & Cut Off

Neg Equ Pos

40002 Ct 40 37-39 3660003 Ct 0 39-40 1-39

Part Units

Chikungunya Virus - Units & Cut Off

Neg Equ Pos

10003 Ct no specific amplification/IC OK specific amplification/IC OK40002 Ct 40 37-39 3660003 Ct 0 39-40 1-3980011 Ct80042 Cp 50 45-50 4580069 Ct 0.00 & above 40.00 - Below 40.0

80071 Cp No product formation at meltingcurve analysis

Product formation at meltingcurve analysis

83000 Ct Below Ct value - Above Ct value89082 Ct 45 40 2989112 No units available

Part UnitsRoss River Virus - Units & Cut Off

Neg Equ Pos

40002 Ct 40 37-39 3660003 Ct 0 39-40 1-39

80071 Cp No product formation atmelting curve analysis


Part Units

Sindbis Virus - Units & Cut Off

Neg Equ Pos

40002 Ct 40 37-39 36


Alphavirus Extraction Kit Information for Survey MAF1:2015:1A, 1B, 1C, 1D, 1E & 1F

PartAlphavirus Group - Extraction

Manufacturer Kit Name Lot No. Expiry Date Extraction Vol Elution Vol

10003 Qiagen QIAamp Viral RNA Mini Kit 148026903 Apr 16 140 6040002 Qiagen EZ1 Virus Mini Kit v2.0 148038536 Mar 16 200 9083000 Qiagen QIAamp Viral RNA Mini Kit 148017918 Jan 16 140 5089112 Qiagen QIAamp Viral RNA Mini Kit 148013001 Nov 15 140 60

Part

Barmah Forest Virus - Extraction


40002 Qiagen EZ1 Virus Mini Kit v2.0 148038536 Mar 16 200 9060003 Qiagen QIAamp Viral RNA Mini Kit 148044563 Aug 16 160 100

Part

Chikungunya Virus - Extraction

Manufacturer Kit Name Automation Lot No. Expiry Date ExtractionVol

ElutionVol

10003 Qiagen QIAamp Viral RNA Mini Kit - 148026903 Apr 16 140 6040002 Qiagen EZ1 Virus Mini Kit v2.0 - 148038536 Mar 16 200 9060003 Qiagen QIAamp Viral RNA Mini Kit - 148044563 Aug 16 160 10080011 bioMerieux NucliSens EasyMag Z015NA Dec 15 200 2580042 Qiagen EZ1 Virus Mini Kit v2.0 - 148046999 Nov 15 200 6080069 Qiagen QIAamp Viral RNA Mini Kit - 148045591 Sep 16 140 6080071 Qiagen QIAamp Viral RNA Mini Kit - 148052920 Oct 16 140 6083000 Qiagen QIAamp Viral RNA Mini Kit - 148017918 Jan 16 140 50

89082 Roche Diagnostics MagNA Pure Compact NucleicAcid Isolation Kit I MagNA Pure 10771500 Dec 15 200 50

89112 Qiagen QIAamp Viral RNA Mini Kit - 148013001 Nov 15 140 60

Part

Ross River Virus - Extraction


40002 Qiagen EZ1 Virus Mini Kit v2.0 148038536 Mar 16 200 9060003 Qiagen QIAamp Viral RNA Mini Kit 148044563 Aug 16 160 10080071 Qiagen QIAamp Viral RNA Mini Kit 148052920 Oct 16 140 60

Part

Sindbis Virus - Extraction


40002 Qiagen EZ1 Virus Mini Kit v2.0 148038536 Mar 16 200 90


Alphavirus Amplification Kit Information for Survey MAF1:2015:1A, 1B, 1C, 1D, 1E & 1F

Part

Alphavirus - Amplification

Manufacturer Kit Name Method Automation Lot No. ExpiryDate

MasterVol

SampleVol

Single/Nested

10003 In house - RT PCR Eppendorf GradientS Thermalcycler NA NA 37 3 Nested

40002 In house - RT PCR Eppendorf GradientS Thermalcycler 19032015 Mar 16 15 5 Nested

83000 BioRad iScript One-StepRT-PCR iQ5 170-8893 Dec 15 20 5 Single round

89112 Roche AppliedScience

Expand HighFidelity PLUS

AB 2720 ThermalCycler 13434025 Mar 14 48.8 1 Nested

Part

Barmah Forest Virus - Amplification


MasterVol

SampleVol Single/Nested

40002 In house - TaqMan RT-PCR Rotorgene 20052014 May 15 15 5 Single round60003 In house - TaqMan RT-PCR Rotorgene NA NA 8 12 Single round

Part

Chikungunya Virus - Amplification


MasterVol

SampleVol

Single/Nested

10003 In house - TaqMan RT-PCR ABI Prism NA NA 17 3 Single round40002 In house - TaqMan RT-PCR Rotorgene 04032015 Mar 16 15 5 Single round60003 In house - TaqMan RT-PCR Rotorgene NA NA 12 8 Single round

80011 Invitrogen SuperScript III qRT-PCR RT PCR Light Cycler 1565698 Apr 16 15 5 Single round

80042 In house - Real-time PCR Light Cycler NA NA 20 5 Single round80069 AITbiotech abTES DEN 5 qPCR RT-PCR CFX96 C151-24 May 15 20 5 Single round80071 In house - Real-time PCR Light Cycler 10035000 Sep 15 9 1 Single round83000 Qiagen OneStep RT-PCR Kit RT-PCR - 148030425 Jul 15 20 5 Single round89082 In house - TaqMan RT-PCR LightCycler 110315 Mar 16 15 5 Single round

89112 RocheDiagnostics

LightCycler RNAAmplification kitHypro

RT-PCR Light Cycler 14748722 Jan 16 15 5 Single round

Part

Ross River Virus - Amplification


MasterVol


40002 In house - TaqMan RT-PCR Rotorgene 05022015 Feb 16 15 5 Single round60003 In house - TaqMan RT-PCR Rotorgene NA NA 12 8 Single round80071 In house - Real-time PCR Light Cycler 10035000 Sep 15 9 1 Single round

Part

Sindbis Virus - Amplification


MasterVol


40002 In house - TaqMan RT-PCR Rotorgene 21012015 Jan 16 15 5 Single round


Alphavirus Detection Kit Information for Survey MAF1:2015:1A, 1B, 1C, 1D, 1E & 1F

Part

Alphavirus Group - Detection

Manufacturer Kit Name Method Automation Lot No. ExpiryDate Single/Nested

10003 In house - Gelelectrophoresis - NA NA Nested

40002 Qiagen QIAxcel DNA HighResolution Kit QIAxcel C131031033 May 15 Nested

83000 BioRad iScript One-Step RT-PCR iQ5 170-8893 Dec 15 Single round

89112 UV Products UV Transilluminator GeneSnap fromSynGene IN HOUSE Oct 15 Nested

Part

Barmah Forest Virus - Detection


40002 In house - TaqMan RT-PCR Rotorgene 20052014 May 15 Single round60003 In house - TaqMan RT-PCR Rotorgene NA NA Single round

Part

Chikungunya Virus - Detection


10003 In house - TaqMan RT-PCR ABI Prism NA NA Single round40002 In house - TaqMan RT-PCR Rotorgene 04032015 Mar 16 Single round60003 In house - TaqMan RT-PCR Rotorgene NA NA Nested80011 In house - TaqMan RT-PCR LightCycler 1565698 Apr 16 Single round80042 In house - Real-time PCR Light Cycler NA NA Single round80069 AITbiotech abTES DEN 5 qPCR RT-PCR CFX96 C151-24 May 15 Single round80071 In house - Real-time PCR Light Cycler 10035000 Sep 15 Single round

83000 Qiagen QuantiTect Probe RT-PCR Kit

Fluorescent(FAM channel) - 148030425 Jul 15 Single round

89082 In house - TaqMan RT-PCR LightCycler 110315 Mar 16 Single round89112 In house - TaqMan RT-PCR LightCycler IN HOUSE Jan 16 Single round

Part

Ross River Virus - Detection


40002 In house - TaqMan RT-PCR Rotorgene 05022015 Feb 16 Single round60003 In house - TaqMan RT-PCR Rotorgene NA NA Single round80071 In house - Real-time PCR Light Cycler 10035000 Sep 15 Single round

PartSindbis Virus - Detection

Manufacturer Kit Name Method Automation Lot No. Expiry Date Single/Nested

40002 In house - TaqMan RT-PCR Rotorgene 21012015 Jan 16 Single round


Flavivirus Controls & In house Methods for Survey MAF1:2015:1A, 1B, 1C, 1D, 1E & 1F

Flavivirus Group - Controls & In house Methods



10003 In house - BVDVspiked into sample NFW MVE cDNA Flavivirus generic

PCR gel electrophoresis

40002 N/A in house NA in house synthetic 28082014 NA Nested RT-PCR band on ethidiumbromide gel

80071 NA NA NA Water NA NA In house (JEV cell lysateRNA as Positive Control) NA NA Adapted from

Moureau et al, 2007QuantiFast SYBRgreen RT-PCR

83000 In-house - NA Non TemplateControl - NA Dengue & JE Virus

Isolates - NASelected humangene as inhibitorcontrol for PCR

-

89112 - - NA Promega (nucleasefree water) 14748722 Jan 2016 In house YF RNA as

pos control NA Conventional RT-PCR

Agarose gelelectrophoresis

Japanese Encephalitis Virus - Controls & In house Methods



10003 In house - BVDVspiked into sample NFW Japanese encephalitis

virus cDNAreal-time JEV specificTaqMan assay Real-time PCR


16012015,16012015 NA Taqman RT-PCR Probe Fluorescence

60003 In-house In-house Reference strain Yes Realtime PCR80069 NA NA NA In house NA NA In house NA NA Conventional RT-PCR Gel Electrophoresis

80071 NA NA NA WATER NA NA In house (JEV cell lysateRNA as Positive Control) NA NA Adapted from S. R.

Santhosh et al. 2007LightCycler® RNAMaster SYBR Green I

83000 In-house - NA Non TemplateControl - NA JE Synthetic

Oligonucleotide - NASelected humangene as inhibitorcontrol for PCR

-



Flavivirus Controls & In house Methods for Survey MAF1:2015:1A, 1B, 1C, 1D, 1E & 1F (continued)

Dengue Virus - Controls & In house Methods



1187

10003 In house - BVDVspiked into sample NFW Dengue virus types 1-4

cDNAIndividual Dengue1-4 TaqMan assays Real-time PCR


02032015,02032015 NA Taqman RT-PCR Probe

Fluorescence60003 In-house In-house Reference strain Yes Realtime PCR80011 In-house NA NA In-house NA NA In-house NA NA

80042 N/A In-house In-house Adapted frompublished method Real time PCR

80052 In-house NA In-house NA In-house DDCtrl-18 NA TaqMan Real TimeMultiplex RT-PCR

TaqMan Real TimeMultiplex RT-PCR

80069 NA NA NA In-house NA NA In-house NA NA - -

80071 NA NA NA Water NA NAIn house (Dengue virusserotypes 1-4 cell lysateRNA as Positive Control)

NA NA Lai et al 2007 LightCycler RNAMaster Hyb Probe

83000 In-house - NA Non TemplateControl - NA Positive DEN 1,2,3,4

Isolates - NASelected humangene as inhibitorcontrol for PCR

-

89082 In-house Mar 15 Mar 16 In-house Mar 15 Mar 16

89112 Altona IC022031401 May 16 Promega (nucleasefree water) 14748722 Jan 2016 Altona C1410 Jan 15

Murray Valley Encephalitis Virus - Controls & In house Methods



40002 N/A in house NA Probe Control, Primer Control 16012015,16012015 NA Taqman RT-PCR Probe fluorescence

60003 In-house In-house Reference strain Yes Realtime PCR80069 NA NA NA In house NA NA In house NA NA Conventional RT-PCR Gel electrophoresis


Flavivirus Controls & In house Methods for Survey MAF1:2015:1A, 1B, 1C, 1D, 1E & 1F (continued)

Kunjin Virus - Controls & In house Methods



40002 N/A in house NA Probe Control, Primer Control 23072014 ,16012015 NA Taqman RT-PCR Probe Fluorescence60003 In-house In-house Reference strain Yes Realtime PCR

West Nile Virus - Controls & In house Methods



40002 Probe Control; Primer Control 22072010,12032013 NA TaqMan RT-PCR Probe Flourescence

60003 In-house In-house Reference strain Yes Realtime PCR80069 NA NA NA In house NA NA In house NA NA Conventional RT-PCR Gel electrophoresis89082 In house Mar 15 Mar 16 In house Mar 15 Mar 16

89112 NA Promega (nucleasefree water) 14748722 Jan 2016 In house (WNV RNA as Positive

Control) WN 2651 TC1 NA NA Conventional PCR(Roche Titan one step)


Yellow Fever Virus - Controls & In house Methods



40002 N/A Inhouse Neg Control NA Primer Control, Probe Control 12122013,12122013 NA Taqman RT-PCR Probe Flourescence

80069 NA NA NA In house NA NA In house NA NA Conventional RT-PCR Gel Electrophoresis

80071 NA NA NA Water NA NA In house (Yellow fever virus celllysate RNA as Positive Control) NA NA Designed in-house

RT-PCR protocolLightCycler® RNAMaster SYBR Green I


89112 NA Promega (nucleasefree water) 14748722 Jan 2016 In house YFV (RNA) as positive

control YF20/11/121 NA NA Conventional RT-PCR(Roche Titan one step)



Flavivirus Units and Cut-Off Values for Survey MAF1:2015:1A, 1B, 1C, 1D, 1E & 1F

Part UnitsFlavivirus Group - Units & Cut Off Values

Neg Equ Pos

10003 PCR product size no specific electrophoresis sizeband/IC OK

specific electrophoresis sizeband/IC OK

40002 band intensity



83000 Ct No band seen - Band seen89112 No units available

Part UnitsDengue Virus - Units & Cut Off Values

Neg Equ Pos

1187 Ct 40 and below - Less than 4010003 Ct no specific amplification/IC OK specific amplification/IC OK40002 Ct 40 37-39 3660003 Ct 0 39-40 1-3980011 Ct80042 Ct 45 40-45 1-4080052 Ct 39 37-39 3780069 Ct 0.00 & above 40.00 - Below 40.0

80071 Cp No amplification is detected atabs.quantification

Amplification is detected atabs.quantification

83000 Ct Below Ct value - Above Ct value89082 Ct 45 40 2989112 No units available

Part UnitsJapanese Encephalitis Virus - Units & Cut Off Values

Neg Equ Pos

10003 Ct no specific amplification/IC OK specific amplification/IC OK40002 Ct 40 37-39 3660003 Ct 0 39-40 1-3980069 - No specific band seen - Specific band seen



83000 Ct Below Ct value - Above Ct value89082 Ct 45 40 29

Part UnitsKunjin Virus - Units & Cut Off Values

Neg Equ Pos

40002 Ct 40 37-39 3660003 Ct 0 39-40 1-39


Flavivirus Units and Cut-Off Values for Survey MAF1:2015:1A, 1B, 1C, 1D, 1E & 1F (continued)

Part UnitsMurray Valley Encephalitis Virus - Units & Cut Off Values

Neg Equ Pos

40002 Ct 40 37-39 3660003 Ct 0 39-40 1-3980069 - No specific band seen - Specific band seen

Part UnitsWest Nile Virus - Units & Cut Off Values

Neg Equ Pos

40002 Ct 40 37-39 3660003 Ct 0 39-40 1-3980069 - No specific band seen - Specific band seen89082 Ct 45 40 2989112 No units available

Part UnitsYellow Fever Virus - Units & Cut Off Values

Neg Equ Pos

40002 Ct 40 37-39 3680069 - No specific band seen - Specific band seen



89082 Ct 45 40 2989112 No units available


Flavivirus Extraction Information for Survey MAF1:2015:1A, 1B, 1C, 1D, 1E & 1F

PartFlavivirus Group - Extraction


10003 Qiagen QIAamp Viral RNA Mini Kit 148026903 Apr 16 140 6040002 Qiagen EZ1 Virus Mini Kit v2.0 148038536 Mar 16 200 9080071 Qiagen QIAamp Viral RNA Mini Kit 148052920 Oct 16 140 6083000 Qiagen QIAamp Viral RNA Mini Kit 148017918 Jan 16 140 5089112 Qiagen QIAamp Viral RNA Mini Kit 148013001 Nov 15 140 60

Part

Dengue Virus - Extraction

Manufacturer Kit Name Automation Lot No. ExpiryDate

ExtractionVol

ElutionVol

1187 Qiagen QIAamp Viral RNA Mini Kit - 148035479 May 16 140 6010003 Qiagen QIAamp Viral RNA Mini Kit - 148026903 Apr 16 140 6040002 Qiagen EZ1 Virus Mini Kit v2.0 - 148038536 Mar 16 200 9060003 Qiagen QIAamp Viral RNA Mini Kit - 148044563 Aug 16 160 10080011 bioMerieux NucliSens EasyMag Z015NA Dec 15 200 2580042 Qiagen EZ1 Virus Mini Kit v2.0 - 148046999 Nov 15 200 6080052 Qiagen EZ1 Virus Mini Kit v2.0 EZ1 BioRobot 148046732 Nov 15 400 6080069 Qiagen QIAamp Viral RNA Mini Kit - 148045591 Sep 16 140 6080071 Qiagen QIAamp Viral RNA Mini Kit - 148052920 Oct 16 140 6083000 Qiagen QIAamp Viral RNA Mini Kit - 148017918 Jan 16 140 50


MagNA Pure Compact Nucleic AcidIsolation Kit I MagNA Pure 10771500 Dec 15 200 50


Part

Japanese Encephalitis Virus - Extraction

Manufacturer Kit Name Automation Lot No. ExpiryDate

ExtractionVol

ElutionVol

10003 Qiagen QIAamp Viral RNA Mini Kit - 148026903 Apr 16 140 6040002 Qiagen EZ1 Virus Mini Kit v2.0 - 148038536 Mar 16 200 9060003 Qiagen QIAamp Viral RNA Mini Kit - 148044563 Aug 16 160 10080069 Qiagen QIAamp Viral RNA Mini Kit - 148045591 Sep 16 140 6080071 Qiagen QIAamp Viral RNA Mini Kit - 148052920 Oct 16 140 6083000 Qiagen QIAamp Viral RNA Mini Kit - 148017918 Jan 16 140 50



PartKunjin Virus - Extraction


40002 Qiagen EZ1 Virus Mini Kit v2.0 148038536 Mar 16 200 9060003 Qiagen QIAamp Viral RNA Mini Kit 148044563 Aug 16 160 100


Flavivirus Extraction Information for Survey MAF1:2015:1A, 1B, 1C, 1D, 1E & 1F (continued)

PartMurray Valley Encephalitis Virus - Extraction


40002 Qiagen EZ1 Virus Mini Kit v2.0 148038536 Mar 16 200 9060003 Qiagen QIAamp Viral RNA Mini Kit 148044563 Aug 16 160 10080069 Qiagen QIAamp Viral RNA Mini Kit 148045591 Sep 16 140 60

Part

West Nile Virus - Extraction


ElutionVol

40002 Qiagen EZ1 Virus Mini Kit v2.0 - 148038536 Mar 16 200 9060003 Qiagen QIAamp Viral RNA Mini Kit - 148044563 Aug 16 160 10080069 Qiagen QIAamp Viral RNA Mini Kit - 148045591 Sep 16 140 60




Part

Yellow Fever Virus - Extraction


ElutionVol

40002 Qiagen EZ1 Virus Mini Kit v2.0 - 148038536 Mar 16 200 9080069 Qiagen QIAamp Viral RNA Mini Kit - 148045591 Sep 16 140 6080071 Qiagen QIAamp Viral RNA Mini Kit - 148052920 Oct 16 140 60





Flavivirus Amplification Information for Survey MAF1:2015:1A, 1B, 1C, 1D, 1E & 1F

Part

Flavivirus Group - Amplification


MasterVol

SampleVol

Single/Nested

10003 In house - RT PCR Eppendorf GradientS Thermalcycler NA NA 37 3 Nested

40002 In house - RT PCR Eppendorf GradientS Thermalcycler 11022015 Feb 16 15 5 Nested

80071 Qiagen QuantiFastSybrGreen RT-PCR LightCycler 2.0 148034258 Sep 15 20 5 Single round

83000 Bioline UK MyTaq One-StepRT-PCR - ABI Veriti MTOS10-

112C May 15 20 5 Nested


Titan One tubeRT-PCR RT-PCR AB 2720 Thermal

Cycler 10774220 Oct 15 40 10 Single round

Part

Dengue Virus - Amplification


MasterVol

SampleVol

Single/Nested

1187 AIT BiotechPte Ltd

abTes TM DEN 4qPCR 1 Kit Real Time PCR Biorad CFX96 C155-26 Aug 15 20 5 Single round

10003 In house - TaqMan RT-PCR ABI Prism NA NA 17 3 Single round40002 In house - TaqMan RT-PCR Rotorgene 18022015 Feb 16 15 5 Single round60003 In house - TaqMan RT-PCR Rotorgene NA NA 12 8 Single round

80011 Invitrogen SuperScript IIIqRT-PCR RT PCR - 1565698 Apr 16 17 3 Single round

80042 In house - Real-time PCR ABI 7500 N/A NA 15 5 Single round

80052 Invitrogen SuperScript IIIqRT-PCR RT PCR - 1507789 Jan 16 20 10 Single round

80069 AITbiotech abTES DEN 5qPCR RT-PCR CFX96 C151-24 May 15 20 5 Single round


LightCycler RNAAmplification kitHypro

RT-PCR Light Cycler 10772000 Aug 16 9 1 Single round

83000 BioRad iScript One-StepRT-PCR iQ5 172-5141 Jul 16 20 5 Single round

89082 In house - TaqMan RT-PCR LightCycler 110315 Mar 16 15 5 Single round

89112 AltonaDiagnostics

RealStar DengueRT PCR kit Real-time PCR Light cycler

Roche 96 2810-K-1410 Jan 15 25 25 Single round

Part

Japanese Encephalitis Virus - Amplification


MasterVol

SampleVol

Single/Nested

10003 In house - TaqMan RT-PCR ABI Prism NA NA 17 3 Single round40002 In house - TaqMan RT-PCR Rotorgene 11022015 Feb 16 15 5 Single round60003 In house - TaqMan RT-PCR Rotorgene NA NA Single round

80069 Qiagen OneStep RT-PCR Kit RT-PCR - 148045112 Sep 15 20 5 Nested

80071 In house - Real-time Light Cycler 10035000 Sep 15 15 5 Single round

83000 Qiagen OneStep RT-PCR Kit RT-PCR - 148030425 Jul 15 20 5 Single round

89082 In house - TaqMan RT-PCR LightCycler 030215 Feb 16 15 5 Single round


Flavivirus Amplification Information for Survey MAF1:2015:1A, 1B, 1C, 1D, 1E & 1F (continued)

Part

Kunjin Virus - Amplification


MasterVol

SampleVol

Single/Nested

40002 In house - TaqMan RT-PCR ABI Prism 29012015 Jan 16 15 5 Single round60003 In house - TaqMan RT-PCR Rotorgene NA NA 12 8 Single round

Part

Murray Valley Encephalitis Virus - Amplification


MasterVol

SampleVol

Single/Nested

40002 In house - TaqMan RT-PCR Rotorgene 07012015 Jan 16 15 5 Single round60003 In house - TaqMan RT-PCR Rotorgene NA NA 12 8 Single round

80069 Qiagen OneStep RT-PCR Kit RT-PCR - 148045112 Sep 15 20 5 Single round

Part

West Nile Virus - Amplification


MasterVol

SampleVol

Single/Nested

40002 In house - TaqMan RT-PCR Rotorgene 05022015 Feb 16 15 5 Single round60003 In house - TaqMan RT-PCR Rotorgene NA NA Single round


89082 In house - TaqMan RT-PCR LightCycler 300414 Apr 15 15 5 Single round


Titan Onetube RT-PCR RT-PCR AB 2720

Thermal Cycler 10774220 Oct 15 40 10 Single round

Part

Yellow Fever Virus - Amplification


MasterVol

SampleVol

Single/Nested

40002 In house - TaqMan RT-PCR Rotorgene 10122014 Dec 15 15 5 Single round


80071 In house - Real-time PCR Light Cycler 10035000 Sep 15 9 1 Single round89082 In house - TaqMan RT-PCR LightCycler 110315 Mar 16 15 5 Single round


Titan Onetube RT-PCR RT-PCR AB 2720

Thermal Cycler 10774220 Oct 15 40 10 Single round


Flavivirus Detection Information for Survey MAF1:2015:1A, 1B, 1C, 1D, 1E & 1F

PartFlavivirus Group - Detection

Manufacturer Kit Name Method Automation Lot No. Expiry Date Single/Nested

10003 In house - Gel electrophoresis - NA NA Nested

40002 Qiagen QIAxcel DNA HighResolution Kit QIAxcel C131031033 May 15 Nested

80071 Qiagen QuantiFastSybrGreen RT-PCR LightCycler 2.0 148034258 Sep 15 Single round

83000 In house - Gel electrophoresis - 0000398806 Nov 15 Single round

89112 UV Products UV Transilluminator GeneSnapfrom SynGene IN HOUSE Oct 15 Single round

Part

Dengue Virus - Detection


1187 AIT BiotechPte Ltd

abTes TM DEN 4qPCR 1 Kit Real Time PCR Biorad CFX96 C155-26 Aug 15 Single round

10003 In house - TaqMan RT-PCR ABI Prism NA NA Single round40002 In house - TaqMan RT-PCR Rotorgene 18022015 Feb 16 Single round60003 In house - TaqMan RT-PCR Rotorgene NA NA Nested80011 In house - TaqMan RT-PCR LightCycler 1565698 Apr 16 Single round80042 In house - Real-time PCR ABI 7500 N/A NA Single round80052 In house - TaqMan RT-PCR Rotorgene 6089093 NA Single round80069 AITbiotech abTES DEN 5 qPCR RT-PCR CFX96 C151-24 May 15 Single round80071 In house - Real-time PCR Light Cycler 10772000 Aug 16 Single round

83000 BioRad iScript One-Step RT-PCR iQ5 172-5141 Jul 16 Single round

89082 In house - TaqMan RT-PCR LightCycler 110315 Mar 16 Single round

89112 AltonaDiagnostics

RealStar Dengue RT-PCR kit Real-time PCR Light Cycler

Roche 96 2810-K-1410 Jan 15 Single round

Part

Japanese Encephalitis Virus - Detection


10003 In house - TaqMan RT-PCR ABI Prism NA NA Single round40002 In house - TaqMan RT-PCR Rotorgene 11022015 Feb 16 Single round60003 In house - TaqMan RT-PCR Rotorgene NA NA Single round80069 In house - Gel electrophoresis - - NA80071 In house - Real-time Light Cycler 10035000 Sep 15 Single round

83000 Qiagen Quantitect ProbeRT-PCR kit

Fluorescent (FAMchannel) BioRad CFX 96 148030425 Jul 15 Single round

89082 In house - TaqMan RT-PCR LightCycler 030215 Feb 16 Single round


Flavivirus Detection Information for Survey MAF1:2015:1A, 1B, 1C, 1D, 1E & 1F (continued)

Part

Kunjin Virus - Detection


40002 In house - TaqMan RT-PCR Rotorgene 29012015 Jan 16 Single round60003 In house - TaqMan RT-PCR Rotorgene NA NA Single round

Part

Murray Valley Encephalitis Virus - Detection


40002 In house - TaqMan RT-PCR Rotorgene 07012015 Jan 16 Single round60003 In house - TaqMan RT-PCR Rotorgene NA NA Single round80069 In house - Gel electrophoresis - NA NA

Part

West Nile Virus - Detection


40002 In house - TaqMan RT-PCR Rotorgene 05022015 Feb 16 Single round60003 In house - TaqMan RT-PCR Rotorgene NA NA Single round80069 In house - Gel electrophoresis - NA NA89082 In house - TaqMan RT-PCR LightCycler 300414 Apr 15 Single round

89112 UV Products UVTransilluminator

GeneSnapfrom SynGene IN HOUSE Oct 15 Single round

Part

Yellow Fever Virus - Detection


40002 In house - TaqMan RT-PCR Rotorgene 10122014 Dec 15 Single round80069 In house - Gel electrophoresis - NA NA80071 In house - Real-time PCR Light Cycler 10035000 Sep 15 Single round89082 In house - TaqMan RT-PCR LightCycler 110315 Mar 16 Single round

89112 UV Products UVTransilluminator

GeneSnapfrom SynGene IN HOUSE Oct 15 Single round

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