regenerative medicine brochure

your strategic partners in developingphysiologically advanced technologies for

RegenerativeMedicine

from Researchto Clinical

Applications

Treatment, Preparation &Harvesting

Engineering &Modification

Culturing & Growth

Physiological Assessment &ConditionMonitoring

Call to receive other catalogs of interest:

BehavioralResearch

HarvardApparatusPumps

Electro-physiology

&CellBiologyResearch

RegenerativeMedicine

Electro-poration

&Electrofusion

MolecularSample

Preparation

Call to receive other catalogs of interest:

phone 508.893.8999 • email [email protected] • web www.harvardapparatus.com2

TableofCon

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Table of ContentsHarvardApparatus

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Regenerative Medicine

Introduction................................................................................................................................................................................................2-5

Overview of Technical Documents................................................................................................................................................................6-9

New Product Summary & Phases ................................................................................................................................................................10-11

Table of Contents for Phases 1-4 ................................................................................................................................................................12

Phase 1: Preparation & Harvesting Overview

Introduction ........................................................................................................................................................................................13-14

Drug Delivery/Infusion, Nanomite Syringe Pump ....................................................................................................................................15

Stem Cell Cooled Injection, NanoCool Delivery System ............................................................................................................................16

Fast Bolus Injection System ..................................................................................................................................................................17

Pulsatile Perfusion, Pulsatile Blood Pump ..............................................................................................................................................18

Electrospinning, Advanced Polymer Mixing Delivery System......................................................................................................................19

Cell Culture Perfusion, PHD ULTRA™ Push/Pull Syringe Pump ..................................................................................................................20

Pressure Controlled Injection Pump ........................................................................................................................................................21

Complex Mixture/Dose Delivery, PHD ULTRA™ Gradient System ................................................................................................................22

Accessories: Syringe Warmers ..............................................................................................................................................................23

Accessories: Microfluidic Valves, Circuits, Chips & Tubing ........................................................................................................................24

Ventilators for All Species ......................................................................................................................................................................25

Phase 2: Engineering and Modification Overview

Introduction ........................................................................................................................................................................................26-27

Cellular Gene Delivery Systems ..............................................................................................................................................................27

• Electroporation, Electrofusion, Cell Injection System, Lipsomat-Liposome Production........................................................................27

Phase 2 Examples................................................................................................................................................................................28

• Molecular Biology, Sample Preparation ........................................................................................................................................28

• Protein-Drug Binding Systems ....................................................................................................................................................28

Phase 3: Culturing & Growth Overview

Introduction ........................................................................................................................................................................................29

InBreath 3D Bioreactor for Hollow Organs Bronchus, Trachea & Blood Vessels ..........................................................................................30-31

LB-2 Lung Bioreactor............................................................................................................................................................................32-33

HPC-3, Hydrostatic Perfusion Chamber for Organ and Tissue ..................................................................................................................34

Cell and Tissue Perfusion, Imaging & Assay Chambers ............................................................................................................................35

Toxin and Chemical Compound Removal, FLOW-Thru Dialyzer ..................................................................................................................36

Phase 4: Physiological Assessment & Condition Monitoring Overview

Introduction ........................................................................................................................................................................................37-38

Blood Gas and Ion Measurements, pH, CO2, O2, & Glucose Probes ..........................................................................................................39-40

Guide for Advanced Metabolic and Physiological Evaluation of Organisms, Organs, Tissues & Cells using IR Contrast Imaging (IR-CI) ............41-43

Visible Light Imaging, Digital Magnification System..................................................................................................................................44

References ................................................................................................................................................................................................45-46

Contact Us..................................................................................................................................................................................................47

Note: For Research Use Only. Not for use in humans unless proper investigational device regulations have been followed.

phone 508.893.8999 • email [email protected] • web www.harvardapparatus.com 3

Harvard Bioscience companies have partneredwith leading global scientists to provide specializedsolutions for 110 years… and we are doing it again!

Introduction

HarvardApparatus|AdvancedSolutionsforRegenerative

Medicine

Harvard Apparatusand the HarvardBioscience familyof companies areuniquely positionedto develop advanced

instrumentation to accelerate Tissue Engineering and Cell Therapytreatments in Regenerative Medicine. From origins in 1901, HarvardBioscience Companies have worked closely with leading global

researchers across many disciplines to produce clinical and researchequipment with…– the highest performance– unmatched quality– expert support

...necessary to meet the demands of cutting-edge life science techniques.In keeping with this tradition, we look forward to working with you todevelop and supply the next generation of tools to solve the new challengesof Regenerative Medicine from the research lab bench to the patient.

Harvard Apparatus Provides Equipment and Expertisefor All Phases of Regenerative Medicine Applications

PHASE 1PREPARATION& HARVESTING

Surgical preparation, anesthesiaand ventilation, surgical procedures,

food and drug delivery, animaland tissue handling, processing

and storage.

PHASE 2ENGINEERING& MODIFICATION

Gene delivery and modification,cell growth, differentiation and

manipulation.

PHASE 3CULTURING & GROWTH

Scaffold preparation,decellularizaton and

Recellularization, tissue seeding,scale-up and growth underphysiological conditions.

PHASE 4PHYSIOLOGICAL

ASSESSMENT & CONDITIONMONITORING

Validation of growth-phase andend-stage organ/tissue construct,Monitoring of organ development

for transplant suitability.

TRANSITION

ANIMALORGAN-TISSUE-CELL

RESEARCH CLINICAL

Equipment& Methodologies

HUMANORGAN-TISSUE-CELL


Harvard Apparatus provides advancedsolutions across the full range of Life Science& Biomedical Research Techniques Utilized inRegenerative Medicine

Introduction

HarvardApparatus|AdvancedSolutionsforRegenerativeMedicine

RESEARCH:COMPOUND SCREENING & DRUG DEVELOPMENT- Protein-drug and protein-protein affinity assays- Infusion pumps for drug, anesthesia, or nutritional delivery- Ion channel and ion transport assays, cellular electrophysiology- Automated animal feeding stations- Amino acid analysis

ORGAN FUNCTION MODELING- Vital sign, Blood Gas and Biopotential Monitoring and Analysis- Ex-vivo Physiology Workstations

MOLECULAR SAMPLE PREPARATION- Desalting samples for Mass Spectroscopy analysis of cell signaling molecules- Isolation of nucleic acid from nuclear membranes- Trace enrich cell metabolic waste products for ID and eventual removal

CELL ENGINEERING AND GENETIC MODIFICATION- Electroporation- Electrofusion- Pneumatic injection- Live cell imaging and manipulation

MICROFLUIDICS- Syringe Pumps with Nanoliter Precision- Pulsatile Pumps for Physiological Simulation- Microelectromechanical systems (MEMS)- Lab-on-a-chip (LOC)- Micro Total Analysis Systems (µTAS)

TISSUE HARVESTING AND SURGICAL PROCEDURES- Surgical instruments; operating tables and accessories

SAFETY PHARMACOLOGY- Isolated organ & tissue perfusion- Controlled environments for microscope-based screening assays- Electrophysiological Monitoring- Telemetric Physiology Monitoring- Cardiovascular Pressure and Pressure-Volume Analysis

REGENERATIVE MEDICINE AND TISSUE ENGINEERING- Lung Bioreactor for Rat and Mouse, LB-2- 3D Bioreactor for Trachea/Bronchus and Blood Vesselsm InBreath- Organ Decellularization and Matrix/Scaffold Recellularization- Cell Therapy Injector, NanoCool- Online Physiological and Metabolic Monitoring- Equipment for Organ Harvesting, Transport and Transplant- Anesthesia and Ventilation for Surgery and Recovery

CLINICAL:- 3D Hollow Organ Bioreactor, InBreath

Advanced Solutions for Regenerative Medicine & Tissue Engineering & Cell Therapy

Phase 1 Phase 2 Phase 3 Phase 4 Phase 5HarvardBioscienceCompanies

PREPARATION &HARVESTING

ENGINEERING &MODIFICATIONS

CULTURING &GROWTH

CONDITIONMONITORING WEBSITE

HarvardApparatus

• IdentificationProducts• Ventilators• Anesthesia• Surgical Tools• Infusion Pumps

• Fast Bolus Injections• Cell Delivery Systems• Iontophoresis• Liposome Prep• Drug Delivery Systems• Capillary Glass

• 3D Bioreactors• Toxin & ChemicalRemoval• Perfusion Systems

• Live Imaging• Blood GasMeasurements• Metabolic Monitoring• Data Aquisitionand Analysis• Feedback &Control Syringes

web:www.harvardapparatus.comphone:+1 508-893-8999

DenvilleScientific

• Spinner Flasks• Centrifuge• Pipetting Systems• Incubators• Shakers• Sterilizers

• Transfection Reagents• Ligation Kits• Standards• Visualization Kits• DNA Polymerase• PCR

• Spinner Flasks• Centrifuges• Pipetting Systems• Incubators• Shakers

• Protein Measurements• ssDNA• RNA• Phosphorus• Enzyme Kinetics

web:www.denvillescientific.comphone:+1 908-757-7577

Hugo SachsElektronik

• Ventilators• Operating Tables• Vital Signal Monitors

• Isolated Organ Baths• Tissue Baths• 3D Bioreactors

• Electrophysiology• CardiopulmonaryMechanics• Data Aquisitionand Analysis

web:www.hugo-sachs.de/phone:0 76 65 - 92 00-0

Biochrom

• MolecularSpectroscopy• Protein Analysis• ds DNA• OligonucleotidePrimers• DNA Melts• Molecular Analysis• Amino Acids Analysis

• MolecularSpectroscopy• Media Monitoring

• MolecularSpectroscopy• Amino Acid Analyzers

web:www.biochrom.co.uk/phone:+44 (0) 1223 423723

WarnerInstruments • Cell Imaging

• pH Monitoring• Spill Sensors• Perfusion Chambers

• Electrodes• Amplifiers• Perfusion Chambers• Imaging Chambers• Ion ChannelMonitoring• Ussing Chambers• Temperature Control

web:www.warneronline.comphone:(203) 776-0664

Hoefer

• Molecular Analysis• Cell Membranes• Cytoplasm ToxinMeasure• Cell SignalingChemicals• Blotting

• MolecularSample Preparation• Electrophoresis• Affinity• Ion Exchange• SEC/GPC• Filtration• Dialysis

web:www.hoeferinc.comphone:+1 508-893-8999

BTX

• Electroporation• Electrofusion• In-vivo Transfection• Pneumatic Injection

web:www.btxonline.comphone:+1 508-893-8999

Panlab/Coulbourn

• Behavioral Apparatus:- Mazes- Rota Rods- Treadmills- Activity Monitors

web:www.panlab.comphone:34 934 750 697web:www.coulbourn.comphone:+1 610-395-3771


Introduction


Medicine

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For Animal, Organ & Cellular Physiologyand Behavioral Research

BEHAVIORALFully integrated research systems covering a range ofpowerful behavioral assays.

PHYSIOLOGYEquipment for virtually any Animal, Organ or Cell BiologyExperiment from animal handling to drug infusion tophysiological monitoring.

For Cell/Tissue Engineering, Imaging and Electrophysiology

phone 508.893.8999 • email [email protected] • web www.harvardapparatus.com

MICROSCOPIC LIVE CELL:IMAGING, INJECTION &ELECTROPHYSIOLOGYAmplifiers, imaging,micromanipulation and perfusion forthe electrophysiological andneurological sciences.

TRANSFECTIONA comprehensive line of instrumentsand accessories for bothelectroporation and electrofusion ofmammalian, bacterial, yeast, fungi,insect and plant cells and tissues.

CELL MODIFICATIONThis guide explains Liposomes,Pneumatic Injection, Iontophoresis,Electroporation and MechanicalInjectors.

Harvard Apparatus has the largest global support network of experts, and broadest range ofspecialized products to assist you in finding the best solutions for your experimental research.

Our HarvardWe have Solutions for

TechnicalDocuments

Ask about our catalogs,technical guides, applicationnotes and bibliographies



Medicine

7

Specialized Tools for Model Organisms and Ex-Vivo PhysiologySystems, like the IH-SR for Isolated Heart Perfusion

ISOLATED HEARTDetailed system guide for the ultimate ex-vivo perfusionsystem for small rodent heart, the IH-SR.

MODEL ORGANISMSFeatures products used specifically for smaller organismsincluding Drosophila, Nematodes, Xenopus and Zebrafish.

Specialized Guides for Bioresearch

EPITHELIAL TRANSPORTDiscusses the theory of operation andpresents the largest collection ofUssing Systems available.

CELLULARELECTROPHYSIOLOGYTOOLSDetailed guide for a range of tools forcell based electrophysiology assays.

BILAYER WORKSTATIONFocus on the Planar Lipid BilayerWorkstation as a foundation for drugscreening as well as ion channelstructure and function.

Apparatus Family:all Regenerative Medicine Research ApplicationsCall 508-893-8999 or email [email protected] for technical support or torequest a catalog or Technical Application Guide.

TechnicalDocum

ents



8

Advanced Organ, Tissue and Cellular Engineering and Therapy Toolsfor Regenerative Medicine

REGENERATIVE MEDICINE3D Bioreactors for Lung, Trachea/Bronchus & Blood Vessels.Stem Cell Injection Systems for Fast Bolus Injection,Pressure-Controlled Fluid Delivery, Active Cooling & Mixing ofCell Suspensions for Injection, Electrospinning.

Ask about our catalogs,technical guides, applicationnotes and bibliographies

Harvard Apparatus has the largest global support network of experts, and broadest range ofspecialized products to assist you in finding the best solutions for your experimental research.

TechnicalDocuments

Smooth High Accuracy Flow,Very High,Very Lowor Physiological Flow Rates

NANO & MICROFLUIDICS, PUMPS & INFUSIONPulsatile Blood Pumps, Peristaltic Pumps, Syringe Pumps and full line of connectors, tubing and accessories.

Our HarvardWe have Solutions for



Medicine

9

Call 508-893-8999 or email [email protected] for technical support or torequest a catalog or Technical Application Guide.

Molecular Biology and Assays, Sample Preparationand Separations

1D AND 2D ELECTROPHORESIS, WESTERN BLOT, IMAGING SYSTEMS, PROTEIN BINDINGSYSTEMS, SPE CLEAN-UP, DIALYSIS CLEAN-UP, PCR REAGENTS, INCUBATORS, MIXERS

BIOCHROM: Amino Acid Analyzers, Full Family of Spectrometers

BTX: The In Vivo and In Vitro Experts in Electroporation and Electrofusion

DENVILLE SCIENTIFIC: A Complete Family or PCR, Incubators and Mixers

HOEFER: The Electrophoresis People, Molecular Sample Preparation

TechnicalDocum

ents

Apparatus Family:all Regenerative Medicine Research Applications


New

ProductSummay


Fast Bolus Injection System for rapid, controlled fluid delivery (p. 17)

Now a system to deliver pressure-controlled fluid injection to intact organs andtissues. The system delivers rapid injections while simultaneously monitoringpressure at the injection site. Measured pressure modulates pump speed fortight control of delivered injection pulse.

NEW Systems for Regenerativeand Tissue EngineeringOur newly developed systems are guided by break-scientists and surgeons to ensure performance,

Clinically proven and published bioreactor design used in successful humantransplantation of bronchus. Produced in conjunction with Politecnico Di Milanoand University of Barcelona, the InBreath bioreactor is ideally suited toregeneration of tubular hollow organs such as trachea/bronchus, blood vessels,esophagus and intestines.

Stem Cell Cooled Injection with high viability,NanoCool Delivery System (p. 16)

Developed in the field, specifically to inject 1 ul to 100 ul of stem cells into livetissue while maintaining cell viability in an accurate and easy-to-use system.The NanoCool cools,cells to 18 degrees in the syringe during injections.

Complex Automatic Mixture/Gradient Delivery & Dosing System (p. 22)

The Harvard Apparatus Mixture/Gradient systems allow you to deliver ComplexMixture and Dosing Protocols by controlling multiple flow streams with variable% concentration automatically. These serial dilutions or changes are pre-programmed using easy set-up Pump Methods. Injection can also be initiatedby a triggering event from another device, allowing feedback and control loopsto be easily implemented. For example, a pH sensor could trigger injection ofbuffer solution to a mixed drug dose in order to maintain pH at a set level.

Stepped

ContinuousFlo

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ate

Time

Ramped

InBreathe Hollow Organ 3D Bioreactor, proven throughhuman transplantation (p. 30-31)



Medicine

11

New

ProductSum

mary

Medicine, Cell Therapy

through research in conjunction with leadingvalidity and scientific relevance.

Dedicated Lung Bioreactor with online pulmonary monitoring (p. 32)

Proven and published whole organ bioreactor specifically designed inconjunction with Massachusetts General Hospital for generation of rodent lungs.Real-time monitoring of respiratory mechanics and Active Ventilation duringgrowth phase for organ performance validation and successful transplantation.

Sterilizable pH, CO2, O2 and GLUCOSE SENSORS (p. 39)

Blood gas and metabolite measurements are critical determinants of organ andtissue viability and performance. These sensors are available in a range of sizesand configurations as well as bioreactor-integrated forms for online monitoring.Sterilizable and made of completely inert material, these sensors are ideal forbioreactor use.

Hand-Held Microscope up to 500X Magnification (p. 59)

Small, light weight digital microscope for Organ and Tissue preparations,microsurgery, injection or cannulation. Remotely placed monitor (or PCConnection) enables simple mounting to bioreactor-based systems.

Harvard Apparatus Partners Program

Harvard Apparatus is partnering with global scientific partners in academia andindustry to license, co-develop or test device concepts that will accelerateregenerative medicine, cell therapy and tissue engineering in the research andclinical marketplace. This proven development model has consistently resultedin refined, successful and relevant commercial products. For development ofadvanced, specialized systems for cell, tissue, organ and in-vivo assays pleasecontact us. Contact Ron Sostek 508-893-8999 x 1100,[email protected]


Phases


Phase 1: Treatment,Preparation & Harvesting1. Identification Products

- Tattoo- Clips- RFID

2. Hair Removal Products3. Surgical Equipment

- Operating Tables & Lights- Magnification Products

4. Surgical Instruments- Tools- Needles- Sutures- Catheters- Sterilization Equipment

5. Anesthesia Equipment6. Ventilation Equipment7. Vital-Sign Monitoring

- Capnographs- Homeothermic Blankets- Pulse Oximeters- Blood Pressure- EKG- Others

8. Recovery Chambers9. Infusion Products

- Drug Delivery- Anesthesia Delivery- Nutrient Delivery

Phase 2: Engineering &Modification1. Cell or Tissue Modification:

In Vivo, In Vitro, Gene Delivery- Pneumatic Femto Injectors- Electroporation- Electrofusion- Iontophoresis- Liposomes- Mechanical Injectors

2. Cell or Tissue Accessories- Capillary Tubes- Capillary Pullers- Capillary Forges- Homogenizers- Manipulators

3. Perfusion Chambers- Imaging Culture Plates

4. Molecular Isolation, Purification Analysis- Amino Acid Analysis- Electrophoresis- Western Blot- Protein-Ligand Interactions- Protein-Protein Interactions- DNA purification Kits

5. Molecular Characterization- Spectrometers

Phase 3: Culturing & TissueGrowth1. Cell & Tissue Preparation2. Cell Growth

- Media Infusion Pumpsa. physiologicalb. continuous flow- Imaging Chambers

3. Cell Sorting- Capillary Glass- Manipulators

4. Cell Harvesting5. 3D Organ Bioreactors6. Spinner Flasks7. Centrifuges8. Pipetting Systems9. Tissue Culture10. Incubators11. Shakers12. Continuous flow pumps

Phase 4: PhysiologicalAssessment & ConditionMonitoringCells1. Microscopic Imaging & PerfusionChambers

2. Microscopic Environmental Controls3. Electrophysiology Products4. Condition Monitors

Tissues5. Isolated Tissue & Perfusion Baths

Organs6. Lung Regeneration Bioreactor7. 3D Bioreactors – Hollow Organs

Sensors8. Ion SensorsAmmonia, Calcium, Chloride, Ethanol,Glucose, Lactate, Nitrate, Nitric Oxide,Nitrite, Peroxide, Potassium,Sodium, Urea

9. Flow10. Temperature11. Pressure12. Force13. pH, O2, CO2

NEW Systems for Advancing RegenerativeMedicine & Tissue Engineering



Medicine

Introduction to Phase 1 Tools, Equipmentand Systems

PHASE 1: Treatment, Preparation & HarvestingPha

se1:Introd

uction

1. Identification Products- Tattoo- Clips- RFID

2. Hair Removal Products3. Surgical Equipment

- Operating Tables & Lights- Magnification Products

4. Surgical Instruments- Tools- Needles- Sutures- Catheters- Sterilization Equipment

5. Anesthesia Equipment

6. Ventilation Equipment7. Vital-Sign Monitoring

- Capnographs- Homeothermic Blankets- Pulse Oximeters- Blood Pressure- EKG- Others

8. Recovery Chambers9. Infusion Products

- Drug Delivery- Anesthesia Delivery- Nutrient Delivery

Preparation & Harvesting of Cells,Tissues & Organs

Phase 1 Catalog and Guides



14

Phase1:Experiments Prepare animal & inject drugs or stems cells

into an organ or tissue to promote regenerationEXAMPLE: CARDIAC INFARCT AND STEM CELL DELIVERY APPLICATION

EXPERIMENTAL GOAL:Stem cells are delivered around a cardiac infract (circled). Four to ten injectionsof volumes in the 5ul range are delivered. Each injection is delivered over a 3second period.

INJECT ANESTHESIA:STEP 1: OPEN CHEST CAVITY

STEP 2: INITIATE INFARCT

STEP 3: INJECT STEM CELLS

STEP 4: CLOSE WOUND AND RECOVERY

Step 1

• Hair Clipper

• Micro VascularOccluder

• Homeothermic Blanket

Step 2, 3 & 4

• Mouse Ventilator

• Mouse & Rat ID Tags

• Stem Cell Delivery Pump

• Bag Restrainers • Magnifying Glasses

• Rat Ventilator

• Sugical Tools

• Hot Bead Sterilizer

• Fiber Optic Illuminator

• Capnograph• Recovery Chamber

Treatment, Preparation & Harvesting:An Experimental ExamplePHASE 1:



Medicine

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Nanom

iteNEW Nanomite for Limited Volume Drug DeliveryHand Held or Stereotaxic Mounted

Figure 1: The blue line above, clearly shows the high accuracy and smoothflow of the Nanomite injection compared to two optimal hand injections.

Superior volume delivery

Figure 2: Tissue creates backpressure but flow is gently ramped so tissue hasa chance to expand to accommodate liquid displacement. You program avolume and time to be delivered, Nanomite does the rest.

Flow ramping injections for optimalspatial cell delivery

Specifications# of Syringes 1Accuracy ±0.5%Cable Length 6 ft (1.8 m)Cable LengthController Dimensions 4-1/2 x 9 x 4-1/2 in (11.4 x 22.9 x 11.4 cm) (H x W x D)Flow Rate Maximum 1900 µl/minFlow Rate Minimum 3.3 nl/hrInjector Head/Actuator 7 x 1-3/8 x 2 in (17.8 x 3.5 x 5.1 x cm) (L x H x W)Pump Function Infusion, withdrawal, volume dispense or continuous pumping modes

Reproducibility ±0.1%Syringe Diameter (Max) 6.00 mmSyringe Size

Maximum 1 mlMinimum 0.5 µl

Syringe Types 1700 , 7000 , 700 and 1000 Hamilton syringes, or anyother syringe manufacturer with known ID in mm

Order # Product70-3601 Nanomite, Infuse/Withdraw

The Nanomite does all the work.You need only to target the areaof interest and step on the footpedal.• Entire flow method recalled and performed• High accuracy and precision cell delivery

system from 1.3pl to 100 µl injections withunmatched performance from up to a1 ml syringe.

• Can be combined with our micro needles downto gauge 37.

• Cells dispensed and next injection ready

Features & Benefits• Light weight makes it ideal for hand-held

stereotaxic injection• Easy-to-use LCD color touch screen with

GUI interface• Multiple methods storage makes runningsimple or complex methods easy

• High performance in a small package• Better performance than manual injections• Version for automatic infusing and withdrawing• Hand-off injections use foot-pedal activation


NanoCool™


SpecificationsAccuracy ±0.5%Alarms End-of Run alarm and over pressure alarmAverage Linear Force 11 to 12 lbsSyringe Diameter (Max) 6.00 mmSyringe Size

Maximum 1 mlMinimum 0.5 µl

Syringe Types 1700 , 7000 ,700 and 1000 Hamilton syringes, or anyother syringe manufacturer with known ID in mm

Injector Head/Actuator 7 x 1-3/8 x 2 in (17.8 x 3.5 x 5.1 x cm) (L x H x W)

NEW NanoCool™ Injector: Fixed 18°C for OptimalCell Viability or Drug Delivery20% increase in survivability, 76% Lower O2 consumptionrate at 18 vs. 37 degrees Celcius

NanoCool™ Cell Delivery System• 18˚C syringe and eppendorf• Flow ramping to reduce injection site blowback• Programmed methods for automatic recall of

entire cell delivery system. Up to 100 methodsstored for recall

• 2 year warranty. Certified with CE, UL,CB Scheme

• High accuracy delivery between 1.3 pl/minto 68ml/min

• Preprogrammed bolus injection mode, justspecify injection size and time of dispense

• Foot pedal start keeps hands free

When injecting stem cells viabilityof cells can be a major issue. TheHarvard Apparatus NanoCool™ isthe only cooled cell injectionsystem with optionalcooled and oxygenated vortexmixer. The injection system andsample vortex holder are held at18°C. The cell delivery is totallyintegrated in it operation throughthe NanoCool’s microprocessorsystem: power, temperatureinjection volumes and flowramping are all controlled by theNanoCool’s program. Keeping thecells at 18°C instead of 37°Creduces O2 consumption of cellsby an average of 76%. The chartbelow shows the different organutilization curves.

Temperature effect on O2 Consumption by Organ Cell Type

Organ Temperature OxygenComsuption

Change in O2Consumption % Change

Kidney 37 to 18 3.7 to 0.9 2.8 75.7

Liver 37 to 18 3.0 to 0.6 2.4 80

Heart 37 to 18 2.4 to 0.7 1.7 70.8

Brain 37 to 18 1.9 to 0.4 1.5 78.9

Muscle 37 to 18) 0.8 to 0.2 0.6 75

Skin 37 to 18 0.4 to 0.1 0.3 75

NanoCool Hand-heldor Stereotaxic

Mounted Injector

NanoCool TouchScreen Controller

Foot-Pedal

Figure 1: IR TemperatureImage NanoCool at 18°C.

Order # Product70-3040 NanoCool™ Injector Infusion/Withdrawal

Programmable Single Syringe



Medicine

17

SpecificationsAccuracy ±0.50%Reproducibility ±0.05%Syringes (Min./Max.) 0.5 µl / 140 mlFlow Rate:

Minimum 1.56 pl/min using 0.5 µl syringeMaximum 215.8 ml/min using 140 ml syringe

Display 4.3" LCD Color Display with TouchpadNon-Volatile Memory Stores all settings

Connectors:# Syringes/Pump 2,4,8,10 (1.4 Liters capacity)Linear Force (Max) 34 kg (75 lbs) @ 100% force selectionPusher Travel Rate:

Minimum 0.18 µm/minMaximum 190.80 mm/min

Dimensions 10.16 x 30.48 x 21.59 cm (4 x 8.5 x 12 in) (H x W x D)Weight 4.5 kg (10 lbs)Regulatory Certifications CE, UL, CSA, CB Scheme, EU RoHS

Fast Bolus Injector for Cell Therapy

Fast Bolus Injector• Inject a fast bolus of large or small volumes in

a second; up to 12X faster than standard pumps• The highest accuracy and precision available• Programmable with multiple methods• Easy-to-use icon interface• CE, ETL, CSA, UL and CB scheme Global

compliance• Delivery from picoliter to hundreds of Ml/min

For delivery of fast infusion or bolus injections into Liver, Kidney or Bonethe Fast Bolus Injector makes it easy. The Fast Bolus Injector can mount a40 ml syringe or larger and accurately inject 100 nl or 100ul per injection withthe push of a foot pedal. This high linear force pump can pump up to 85to 433 pounds of force, easily tp inject viscous materials cell suspensionsor pastes.

Also a full selection of research surgical products are available: Boneaspiration and infusion needles, syringes, surgical equipments,anesthesia circuits, surgical drapes and sponges, ventilators capnographs andpulse oximeters, catheters, sutures, bone drill and more.

Bolus Delivery Programming, it is as easy as setting the amount to be deliveredand the time frame you want to deliver i.e 5ul in three seconds.

Human Bone Marrow–Derived Mesenchymal Stem Cells for IntravascularDelivery of Oncolytic Adenovirus 24-RGD to Human Gliomas, [Cancer Res2009;69 (23):8932–40] .

Multiple methods can be stored and recalled via touch of a button, making theFast Bolus Injector the ultimate, easy to use syringe pump. Methods can beemailed and downloaded from your PC into the pump. Share a method with acolleague or duplicate a complex experimental set-up with an e-mailed method.

Order # Product70-3045 Fast Bolus Injector

FastBolus

InjectorFigure 1: Bone Marrow Aspiration & infusion Needles

Figure 2: Bone Marrow Injection Sites. Figure 3: Catheter placed into righthepatic vein.


PulsatileBloodPump


SpecificationsAccuracy ±0.35%Reproducibility ±0.05%Syringes (Min./Max.) 0.5 µl / 140 mlFlow Rate:

Minimum 1.56 pl/min using 0.5 µl syringeMaximum 220.97 ml/min using 140 ml syringe

Display 4.3" LCD Color Display with TouchpadNon-Volatile Memory Stores all settings

Connectors:# Syringes/Pump 2,4,8,10 (1.4 Liters capacity)Linear Force (Max) 34 kg (75 lbs) @ 100% force selectionPusher Travel Rate:

Minimum 0.18 µm/minMaximum 190.80 mm/min

Dimensions 10.16 x 30.48 x 21.59 cm (4 x 8.5 x 12 in) (H x W x D)Weight 4.5 kg (10 lbs)Regulatory Certifications CE, UL, CSA, CB Scheme, EU RoHS

Pulsatile Blood Pump for Generationof Physiological Flow

Pulastile Blood Pump• Pulsatile output truly simulates the ventricular

action of the heart• Minimal hemolysis• Models for mice to large animals• Ideal for moving emulsions, suspensions, and

non-Newtonian fluids such as bloodIt truly simulates the pumping action of the heart. It features silicone rubber-covered heart-type ball valves and smooth flow paths which minimizehemolysis. Only inert materials like silicone rubber, acrylic plastic, and Tefloncontact the fluid. The pumping head is easy to take apart and reassemble andcan be sterilized.

Outstanding PerformanceThe pulsatile output closely simulates the ventricular action of the heart. Thisaction provides physiological advantages in blood flow for perfusion incardiovascular and hemodynamic studies. It is ideal for isolated organperfusion, whole body perfusion, blood transfers, hydration/dehydrationprocedures and blood cellular profile studies.

Pump MechanismA positive piston actuator and ball check valves provide the proportioningaction. The product of stroke rate times stroke volume is an accurate indicatorof the flow rate. Positive piston action prevents changes in flow rates,regardless of variations in resistance or back pressure. The piston alwaystravels to the end of the ejection stroke, independent of the volume pumped.The Pump completely empties at each cycle.

Order # Product55-3305 Pulastile Blood Pump

Figure 1: A Novel Culture System Shows that Stem Cells Can be Grown in 3Dand Under Physiologic Pulsatile Conditions for Tissue Engineeringof VascularGrafts,Oscar Abilez, et al Journal of Surgical Research 132, 170–178 (2006)doi:10.1016/j.jss.2006.02.017.

Pressure and Flow Curves Using Harvard Apparatus Model1421 Pulsatile Blood Pump in Isolated Perfusion of LeftLower Lobe of Dog Lung

Figure 2: Pa = Pulmonary, Artery, Pressure; Pv = Pulmonary, Venous, Pressure;Qpa = Pulmonary Artery Blood Flow. Istrumentation: Pressure (Statham), Flow(Blotronex Electromagnetic Flowmeter, Recording (Electronics for Medicine.

Note: The above data is supplied through the courtesy of Cardiorespiratorylaboratory Columbia-Presbyterian Medical Center New York, New York, Dr.Alfred P. Fishman, Director.



Medicine

Electrospinning of Novel Scaffold Fiber Materials

ElectrospinningIn 1934, a process was patented by Formhals, wherein an experimental designwas outlined for the production of polymer filaments using electrostatic force.When used to spin less than 100 micron Fibers to micron fibers, the process istermed “electrospinning.” The electrospinning method uses a high voltage tocreate an electrically charged jet of polymer solution or melt, which then driesor solidifies to leave a polymer fiber. This is accomplished by first mounting asyringe into a syringe pump capable of delivering a constant pressure andsmooth flow to a pulled spray nozzle. One electrode is placed into this spinningsolution/melt while the other is placed onto a grounded collector, most often ametal wire mesh. The driving force is provided by a high voltage sourcegenerating up to 30kV of positive or negative polarity through the electrodelocated at the nozzletip. When an electricfield is subjected tothe end of the nozzletube a charged isinduced on thesurface of the liquid.A mutual chargerepulsion causes aforce directlyopposite to thesurface tension, suchthat as the intensityof the electric field isincreased, thehemispherical surface of the fluid at the nozzle tip elongates into a conicalshape known as the Taylor cone. With increasing field, a critical value isattained when the repulsive electrostatic force overcomes the surface tensionand a charged jet of fluid is ejected from the tip of the Taylor cone. The jetundergoes a whipping process during which the solvent evaporates and theremaining charged polymer fiber randomly lays itself on the grounded metalcollecting screen. By adjusting the flow of the fluid from the pump and themagnitude of the electric field, the spinning rate can be controlled.

Polymersolution

Jet

Pipette

Taylor cone

High Voltage Supply

Syringe Pump

Collector Screen(Rotating or Stationary)

Variables:1. Molecular Weight, Molecular-Weight Distribution and Architecture2. Solution Properties: Viscosity, Conductivity, Surface Tension3. Electric Potential, Flow Rate, Collection Screen4. Distance between capillary and collection screen5. Ambient Parameters: temperature, humidity, chamber air velocity6. Motion of the Target Screen (collector) and chamber Air Velocity

The Harvard Apparatus Family of Pumps iswell-suited for electrospinning:• Regulatory compliance ensuring electrical

currents do not damage pumps with an optionfor a custom grounding cable for added userprotection

• Deliver the smoothest and most accurate flowsso that uniform fibers are created withoutdroplet formation

• Programmable flow rates with automatic timecontrol; timed length of spin, optimizationprotocols can be created, powerful enough forviscous solutions, highest accuracy at thelowest (nanoliter) flow rate range

• Rugged design with 2 year warranty andglobal support

Electrospinning

Figure 3: Photograph of a meniscus ofpolyvinyl alcohol in aqueous solution showinga fibre being electrospun form a Taylor cone.

Figure 2: (A) Vessel Scaffold, (B) Scaffold for Muscle, (C) Tissue Scaffold, (D) Bone Scaffold, (E) Organ Scaffolds.

A B C D E

Figure 1: Schematic of the Electrospinning setup.

Figure 4: AdvancedPolymer MixingDelivery System

Order # Product70-3007 PHD ULTRA™ Programmable Syringe Pump


PHDULTRA™Push-Pull


Continuous Flow for Nutrient Delivery Systems

PHD ULTRA™ Push-PullProgrammable Syringe Pump• Compensating Flows

- The control of continuous infusion andsimultaneous withdrawal of liquids whilemonitoring fluid levels

• Perfusion Across Tissue Beds- Directional control of pulseless flow acrossa tissue bed using switching valves

• Continuous Flow with High Accuracy andSmooth Flow- Pump Any Volume Large or Small withSmooth, Non Pulsating Flow

• Continuous Accurate Flow for HighPressure systems- Unlike peristaltic pumps, syringe pumps canpump against high pressures

• Easily Sterilized Flow Path- By replacing syringe and tubing with asterilized set, this pump can maintain it’ssterility

The continuous flow pump can provide low pulsation, high accuracy andprecise delivery of flow to any reactor set-up. When you are trying to providelow shear stress, non pulsing flow during the adhesion step of regeneration ofcell deposition for tissue or organ development, the syringe pump provide thecontinuous Flow compensation of a peristaltic, with higher accuracies andprecisions. These flows can be cooled or heated with in –line heaters orcoolers, These pumps have a rugged construction which allows the to work forlong periods of time with high reliability. These pumps come with a two yearwarranty as a testament to their durability.

Order # Product70-3009 PHD ULTRA™ Push-Pull Programmable Syringe Pump

Figure 1: Pressure-driven flow was continous using a programmable push-pullsyringe pump (Harvard Apparatus, Holliston, MA). Media was equilibriated with10% or 21% O2 in a gas exchanger made with gas-permeable silastic tubing.


PressureDelivery

InjectionSystem


Medicine

Pressure Controlled Injection System

Pressure 1

Pressure 2

Pressure 3

Pressure 4 Pressure 5

•Directly measure pressure at site of injection•Simultaneously measure overall system pressure•Option for holding injection pressure constantfor defined time period

The system is specifically designed for repeatable, pressure controlledinjections into intact organs. However a wide range of settings and accessoriesmakes the system appropriate for virtuallly any semi-rigid substrate and certainhollow-body systems.

Figure 1: Pressure Delivery Injection System

Figure 2: System components include a syringe pump, injectionpathway, pressure measurement hardware, and data acquisitionand feed back system. Injection parameters are programmedwhile pressure is continuously monitored. Real time pressurefeedback modulates syringe pump to keep pressure constant.

Order # ProductPressure-Controlled Injection System Parameters Controller77-0000 ML866 POWERLAB 4/304 Channel w/LABCHART Software

to Measure System Pressure after Syringe72-4496 Research Grade Blood Pressure Transducer,

115VAC/60Hz, with 6 ft cable

Blister Pressure Measurement72-9845 1F SGL Pres CTH 120 cm No Rep

Syringe Pump Capacity 2X up to 140 ml Syringes Max Flow Ratewith 2X 140 ml Syringes is 3.68 ml/sec per Syringe or 7.36 ml/sec70-3007 PHD ULTRA™ I/W Programmable70-3033 PHD ULTRA™ Analog Central Imput

Order # ProductConstant Pressure Injection73-1523 PLUGSYS Minicase Type 60973-2806 SCP PLUGSYS Servo Control F/Perfusion with Interface

Cable for PHD ULTRA™ Control73-0065 TAM-A HSE PLUGSYS Transducer Amplifier Module

Type 705/1, Analog Display72-9843 Pressure Catheter to Tam Cable

Accessories72-2673 Y-Connector FLL/FLL/MLL (Rotating), Package of 2572-14553-WAY FLL-FLL-FLL "T" CONNECTOR, POLYPROPYLENE, PK/2573-0500 Lab Stand w.Heavy Triangular Base Plate 8 mm OD Rod,

30 cm Long. Also includes Acrylate. Block Clamp (73-0566)W/8 & 10 mm Opening

72-9523 MLL to MLL Connector77-0156 Manual Press Cal Kit


Mixture/DoseDeliverySystem


Complex Mixture/Dose Delivery System

The new Harvard Apparatus Syringe Pump Gradient System has the ability toeasily program step and continuous concentration gradients from two or threesolvents (Figure 1). The Harvard Apparatus syringe pumps are highly publishedfor their superior flow accuracy and precise flow performance from ul/min tohundreds of ml/min . Figure 2 shows the shows the flow at 1000 and 500ul/min. In both stem cell bolus injections and 3D reactor perfusion streamdosing, the Harvard Apparatus Gradient systems allows you to automaticallydeliver injections fluids or perfusion flow streams that can have variable %concentration automatically. These serial dilutions or solution changes arepreprogrammed or signaled and initiated by an I/O event. In the case ofinjections you could automatically change the amount of cells in every injectionby programming an increase in the concentration of cell solution in eachinjection, or by simply selecting a new pre-established method. In perfusions, apH probe could signal for a different % composition of the perfusion stream, ordose a perfusion stream automatically. This could provide more bufferingcapacity or adjustment solution to the perfusion stream completely unattended.

This new capability of Harvard Apparatus syringe pumps to completely deliver% composition changes automatically with high accuracy and precision flowprovides a new economical tool to advance experiments and eliminate manualinput for complex solution changes.

- Enzyme studies

- Reaction dosing solutions

- Drug infusion experiments

- Nutritional infusion experiments

- Mixing polymer in electrospinning

- Chromatography & FIA systems

Figure 2: 1000 nl/min & 500 nl/min with 250 µl syringe Measured on a SensirionFlow Sensor (PHD ULTRA)

Stepped

Continuous

Flow

Rate

Time

Ramped

Figure 1: Stepped, Continuous, and Ramped gradient.

Figure 3: The following screen shows the set-up for a gradient.

Order # Product70-4101 PHD ULTRA Gradient System 1 Master/1 Satellite with Stand70-4102 PHD ULTRA Gradient System 1 Master/2 Satellites with Stand70-4106 PHD ULTRA Gradient System 1 Master/1 Satellite without Stand70-4107 PHD ULTRA Gradient System 1 Master/1 Satellite without Stand


Accessories


Medicine

AccessoriesSyringe and In-Line Temperature Controllers

SpecificationsHeater Resistance 18Ω

Voltage Requirement Variable to 12 V maximum

Temperature Range Ambient to 65°C

Temperature Accuracy ±1°C

Cable Length 2.4 m

Warranty One year

Order # Model Product64-1584 SWS-10 Syringe Heater for 10 cc Syringes64-1560 SWS-60 Syringe Heater for 60 cc Syringes64-1585 SWS-140 Syringe Heater for 140 cc Syringes64-1545 TC-124A Temperature Controller, 120 VAC US64-1545E TC-124AE Temperature Controller, 240 VAC Europe64-1655 TC-144 Temperature Controller64-1606 BAC-1 Battery Adapter Cable

In-line solution heating has proven to be one of the most effective methods ofmaintaining the temperature of perfusion solutions. The SC-20 Dual In-lineSolution Heater/Cooler utilizes Peltier thermoelectric devices to regulatetemperature both above and below ambient levels.

The SC-20 is designed to thermally regulate one or two solutions at the sametemperature. Solution temperature can be maintained at 0°C at flow rates of 2ml/min., 5°C at 5 ml/min., or as high as 50°C at 5 ml/min.

An integral water jacket is used to remove excess heat from the SC-20 peltierdevice. Running water either from a tap or a large reservoir can be used. Flowrates as low as 4 liters per hour are sufficient to maintain cooling efficiency.

The SC-20 can be used with either one or two discrete perfusate solutions, orwith a solution/gas combination. When coupled with a PHC Series ImagingChamber Heater/Cooler Jacket, the SC-20 provides an effective means oftemperature control in a Warner chamber, even in the absence of solution flow.

Each SC-20 is supplied with a TA-29 Thermistor Cable Assembly for monitoringthe bath temperature during use, 10 feet of PE-160 tubing and 10 feet of 1/8" I.D.x 1/4" O.D. Tygon tubing.

SC-20 Dual In-line SolutionHeater/Cooler• Heats and cools from 0° to 50°C• Compatible with Warner Series 20 Chambers• Optimized for use with the CL-100 Bipolar

Temperature Controller

SpecificationsMinimum Temperature 0°C (2 ml/min. max flow)Maximum Temperature 50°CMaximum Flow Rate at 5°C 5 ml/min.Accuracy ±0.1°CInternal Dead Volume 330 µlPerfusion Lines Type 316 Stainless Steel, 0.032 in ID x 0.062 in ODWater Jacket Ports Type 316 Stainless Steel, 0.12 in ID x 0.147 in ODController Model CL-100 Bipolar ControllerPhysical Dimensions:

Body (D x L) 21 x 165 mmWeight 109 gCable Length 1.9 m

Connector Type 15 pin Male “D”Warranty One year

Order # Model Product64-0353 SC-20 Solution Heater/Cooler Two Line64-0352 CL-100 Bipolar Temperature Controller

Replacement Parts64-0107 TA-29 Cable with Bead Thermistor for Heater Controllers

SWS-10, SWS-60 & SWS–140SyringeWarmers• Independent temperature control for

individual syringes• Designed for use on a syringe pump

or support stand• Accommodates 10, 60 and 140 cc syringes• Scale marking ports permit volume

monitoring during use• Can be powered from 12 volt battery for

sensitive electrophysiology applications

Syringe Warmer Model Specifications

Model Weight Length OD ID Syringe Type

SWS-10 32.7 g 38.2 mm 22.2 mm 16.2 mmDickerson Becton

SWS-60 76 g 83.7 mm 35.0 mm 29.1 mmDickerson Becton

SWS-140 192 g 109.5 mm 51.0 mm 41.4 mm Monoject

Syringe warmer ona syringe pump

Syringe warmermounted on asupport stand


Accessories


AccessoriesMicro Valves & Micro Connectors

Manifold/Connectors,Needles & TubingTubing, Connectors and ManifoldsHarvard Apparatus has a wide range of tubing, connectors and manifolds soyou can create the fluid pathway to meet your specific requirements. We offermany types of tubing of different materials and sizes. Our fluid manifolds startat 2 channels and go all the way up to 24 channels. We have a multitude ofconnectors that allow you to complete the fluid connections and complete yoursystem. Please call our technical support staff for additional information.

Valves/Controllers(Fluids & Pressure) & Glassor Plastic Fluidic Chips

Routing fluids can be one of the most time-consuming tasks of microfluidicsresearch. CapTite microminiature components take the pain out ofconstructing setups. Based on components developed by Sandia NationalLaboratories, these compact components for 360 µm and 1/16 in tubing aredesigned for leak-free connectivity, even at high pressures.

• Compact components for 360 µm, 1/16,and 1/32 inch in tubing

• Fast, breadboard-based design• Leak-free high pressure setups

Microfluidic Construction KitQty Order # Model ProductSingle User Kit

72-0426 C360-KIT1 Microfluidic Construction Single User Kitfor 360 µm capillary tubing

Kit Components: Includes Tools A, B, C, D, E (1 each)

75 72-0431 C360-100 One-Piece Fitting10 72-0433 C360-101 Plug5 72-0438 C360-203 Tee Interconnect5 72-0443 C360-204 Cross Interconnect10 72-0449 C360-300 Luer-Lock Adapter25 72-0448 C360-400 Bonded Port Connector2 72-0445 C360-500 Selector Valve2 72-0430 LS-600 BreadboardInterface Kit

72-0427 C360-KIT2 Microfluidic Construction Interface Kit for360 µm capillary tubing

Kit Components: Includes Installation Tools A, B, D, E (1 each)

50 72-0431 C360-100 One-Piece Fitting5 72-0433 C360-101 Plug5 72-0438 C360-203 Tee Interconnect5 72-0443 C360-204 Cross Interconnect10 72-0449 C360-300 Luer-Lock Adapter2 72-0445 C360-500 Selector Valve2 72-0430 LS-600 Breadboard

Micro Fluidic Circuits & Chips

• Fast• Cost-effective• Preparation of microfluidic volumesUntil now, commercially available options for preparing microliter volumes havesuffered major drawbacks, including high back pressure, clogging, leakage andlimited surface chemistry options. New CapTite cartridges offer customizablepacking and re-use, for easy sample preparation in both prototypes and high-throughput, automated systems.

In-Line PEEK cartridge forToxic removal


Accessories


Medicine

Harvard Apparatus Ventilators & AnesthesiaThe World’s Most Published Ventilation Systems forStem Cell Harvesting

Look for the complete line of products in the NEW Havard ApparatusAnimal, Organ and Cell Physiology catalog.

• ANESTHESIA• CIRCUITS• SCAVENGERS• DEWARS• INFUSION PUMPS• ANIMAL PREPARATIONTOOLS

• ID SYSTEMS• HAIR REMOVAL• RESTRAINERS• ANIMAL TEMPERATURECONTROL

• MAGNIFICATIONPRODUCTS

• OPERATING TABLES• OPERATING LIGHTS• RECOVERY CHAMBERS• SURGICAL TOOLS

• SUTURES AND NEEDLES• SCAPELS• FORCEP• STERILZERS• VITAL SIGN MONITORS• BLOOD FLOW• CAPNOGRAPH• EEG• GLUCOSE• HEART RATE• O2• OXIMETERS• pH• PULSE RATE• TEMPERATURE

Minivent for Mice 683 for Mice & Rats

Inspira for Mice up to Cats

Anesthesia Machine

26

Phase2:Introduction


Introduction to Phase 2 Tools, Equipmentand Systems

1. Cell or Tissue Modification:In Vivo, In Vitro, GeneDelivery

- Pneumatic Femto Injectors- Electroporation- Electrofusion- Iontophoresis- Liposomes- Mechanical Injectors

2. Cell or Tissue Accessories- Capillary Tubes- Capillary Pullers- Capillary Forges- Homogenizers- Manipulators

3. Perfusion Chambers- Imaging Culture Plates

4. Molecular Isolation,Purification Analysis

- Amino Acid Analysis- Electrophoresis- Western Blot- Protein-Ligand Interactions- Protein-Protein Interactions- DNA purification Kits

5. Molecular Characterization- Spectrometers

Cell, Tissue Engineering, Modification,Preparation & De-Cellularization

PLI-100A femtoliter cell injector


phone 508.893.8999 • email [email protected] • web www.harvardapparatus.com

PHASE 2: Engineering &Modification


Phase2:Introd

uctionHarvardApparatus|AdvancedSolutionsforRegenerative

Medicine

ASK FOR AFREE GUIDESend email to:[email protected]

PLI-100A: CELL INJECTION SYSTEM LIPOSOMAT-LIPOSOME PRODUCTION

ELECTROPORATION HIGH-THROUGHPUTIN VIVO, IN VITRO

ELECTROFUSION & ELECTROPORATION

PHASE 2: Engineering &ModificationHarvard Apparatus is the Leader in Femoliter to MicroliterCell Injection & Transfection Products for Optimal Cell andTisssue Engineering


Phase2:Examples


A Range of Application Areas for Phase 2Tools, Equipment and Systems

MOLECULAR BIOLOGY

• Electrophoreses (1D,2D)• Molecular BiologicalSample Prep Tools

- Chromatographic- Dialysis- Equilibrium Dialysis

PROTEIN-DRUG BINDING STUDIES• No Force Flow• O2 Pressure CO2

• pH Calcium Urea• Temperature, Lactate & More

ASK FOR A FREE GUIDESend email to:[email protected]

Engineering &Modification,Application ExamplesPHASE 2:

SAMPLE PREPARATION• Spectrometers• HTS Plate Readers• Amino Acid Analysis


Phase3:Introd


Medicine

Introduction to Phase 3Tools, Equipment & SystemsThis section contains systems and tools for the regeneration and growth of engineered or natural scaffolds and cellularconstructs. Complete bioreactor systems typically include one or more growth chambers for the construct as well as fluidhandling pathways for culture media perfusion and cell seeding. Probes for monitoring physiological parameters are typicallyincluded, though these are discussed in more detail among the Phase 4 products.

1. Cell & Tissue Preparation2. Cell Growth

- Media Infusion Pumpsa. physiologicalb. continuous flow- Imaging Chambers

3. Cell Sorting- Capillary Glass- Manipulators

4. Cell Harvesting

5. 3D Bioreactors6. Spinner Flasks7. Centrifuges8. Pipetting Systems9. Tissue Culture10. Incubators11. Shakers12. Continuous flow pumps

Cell Culturing & Tissue Growth


PHASE 3: Culturing & Growth


InBreath


InBreath 3D Bioreactor for Hollow Organs,Bronchus, Trachea & Blood VesselsProven through Research and Human Clinical Transplantation

Easy-to-use Controller• Compact design for remote placement

(i.e. outside the incubator)

• The rotational speed can be controlled from0 to 5 rotations per minute

• Motor overload indicator for safe operation

• CE certified

Purpose-Built Reactor Chamber(Custom configurations available)• Reactor container and spindle are made of

Polysulphone, Teflon and 316 Stainless Steelallowing for:- sterilization- chemical inertness- biological compatibility- transparent for excellent visualization

• Chamber has quick-fit spindle for easy removalof the spindle and organ construct for analysisor dissambly

• Rotating spindle assures even exposure tonutrient media

• Integrated ports on spindle allow access tointernal (lumen) surface of organ

• Compact dimensions allow placements ofmultiple units within standard incubator

• Offset cover allows for oxygenation of sampleby non sheering ambient air contact

• Quick release and disassembly of parts makessterilization easy

Benefits & Features• Facilitates cell seeding procedures on both

sides of a 3D tubular matrix, ensuringhomogeneous plating

• Proven Design - demonstrated regeneration ofhuman bronchus and successful humantransplantation with positive clinical outcome;as recently published by Macchiarini et al(2008) Clinical transplantation of a tissue-engineered airway, The Lancet, Volume 372,Issue 9655

• Allow seeding and culturing of different celltypes on either side of the tubular scaffold

• Enhance oxygenation of the culture mediumand mass transport (oxygen, nutrients andcatabolites) between the medium and theadhering cells

• Stimulates the cells with hydrodynamic stimuli,favoring the metabolic activity and thedifferentiation process

• Allows the achievement and maintenance ofsterility and other criteria of Good LaboratoryPractice (GLP), simplicity and convenience

• Permits the possibility of automation andscale-up/-out

In Breath is a rotating, double-chamber, bioreactor designed for cell seedingand culturing on both surfaces of a tubular matrix and includes rotationalmovement of the scaffold around its longitudinal axis. A polymeric culturechamber houses the biologic sample and the medium for the whole cultureperiod. Cylindrical scaffold holders are constructed with working ends ofdifferent diameters - to house matrices of diverse dimensions - and a centralportion of smaller diameter to expose the luminal surface of the matrix for cellseeding and culturing. A co-axial conduit links the inner chamber to theexternal environment through an appropriate interface at the chamber wall.This provides access to seed and feed the luminal surface of the construct.Secondary elements moving with the scaffold holder induce continuous mixingof the culture medium to increase oxygenation and mass transport. Thecell/matrix construct is moved by a DC motor (0-5 rpm adjustable) separated

from the culture compartments. The connection between the motion unit andthe culture chamber allows the first to remain in the incubator for the wholeculture period, moving the chamber independently every time is needed (i.e.sampling, medium exchange). An external control unit regulates and monitorsrotation. Autoclavability, ease of handling under sterile conditions, reliabilityand precision ensure the full compatibility of the device with the GLP rules(Good Laboratory Practice).


InBreath


Medicine

InBreath 3D Bioreactor for Hollow Organs,Bronchus, Trachea & Blood Vessels(continued)

InBreathThough it integrates several sophisticated features, the design of the InBreathis straightforward producing a very effective 3D Bioreactor.

Note: Images Courtesy of P. Macchiarini,University of Barcelona and S Mantero,Politecnico di Milano

SpecificationsRotational Speed 0 - 5 RPMDiagnostics Positional MonitoringMaterials Polysulphone, Teflon, 318 Stainless SteelValidation CEPower 100 - 240 VAC, 50/60 Hz

Order # Product73-4145 InBreath 3D Bioreactor for Hollow Organs, Bronchus,

Trachea & Blood Vessels

References: Macchiarini et al (2008) Clinical transplantation of a tissue-engineered airway, The Lancet, Volume 372, Issue 9655

Figure 1: Tissue-specific tissue spindles ensure proper fit to the rotating corefor even exposure to bathing media.

Figure 2: Sealable access port throughthe chamber wall creates a conduit to thehollow-milled tissue spindle enablingclean access to the bronchial lumen forseeding, in this case, with endothelialcells from the host.

Figure 3: The semi-circular“half pipe” chamber floorcreates a shallow well forexternal seeding, |here withhost chondrocytes derived frombone marrow stem cells of thehost. Mixing posts on therotating tissue spindle maintaincells in suspension for evendeposition on the bronchialsurface. (Figure 3)

Note: For Research Use Only. Not for use in humans unless properinvestigational device regulations have been followed.”



NEW LB-2 Bioreactor for Mouse andRat Lung Regeneration

LB-2 BioreactorFor regeneration and respiratory monitoring of Mouse andRat Lungs from decellularized scaffolds

Benefits & Features:• Proven design – demonstrated regeneration of

rat lung with physiological performance andsubsequent transplantation, as recentlypublished by Ott et al, 2010, Regeneration andorthotopic transplantation of a bioartificial lung, Nature Medicine, Volume 16 No 8, pgs 927-933

•Active control of both airway ventilation andvascular perfusion during regeneration

•On-line monitoring of respiratory mechanics andvascular physiology

•Self-contained bioreactor specially designed forunique lung physiology

Physiological maintenance and monitoring:The LB-2 provides an optimal, physiologically relevant environment for organgrowth as well as the ultimate foundation for real-time monitoring ofrespiratory mechanics. The complete range of pulmonary monitoring parametersare fully integrated into the bioreactor to enable physiological monitoring duringorgan generation and end-stage validation for transplant.

• Airway Flow

• Airway Pressure

• Intrapleural Pressure

• Tidal Volume

• Minute Volume

• Pulmonary Resistance

• Pulmonary Compliance

• Pulmonary Artery Pressure

• Pulmonary Venous (Left Atrial) Pressure

• Vascular Resistance

The LB-2 represents the evolution of legendary Harvard Apparatus - Hugo SachsElektronik perfusion technology from acute ex-vivo perfusion to Bioreactor

based Organ Regeneration. Over 60 years of whole organ physiologicalperfusion experience are at the core of this first commercially availablebioreactor for Lung Regeneration.

Like all Harvard Apparatus - Hugo Sachs Elektronik perfusion systems, the LB-2provides unmatched physiological maintenance and monitoring capabilitiesthanks to the patented Solid State Physiological Perfusion Circuit (S2P2C)technology. In the LB-2, this means rigidly milled pathways, directly into thePerspex structure, for both vascular perfusion and airway ventilation. The resultis precisely repeatable non-turbulent perfusion and respiration characteristics.This combined with the naturally excellent thermal properties of Perspex,creates a system that allows control, maintenance, and monitoring of vascularand respiratory mechanics during regeneration in a way that is morephysiologically relevant than any conventional bioreactor.

System Accessories:• Small Animal Ventilator

• Pulsate Blood Pump

• Syringe Pump

• Transducers

• Amplifiers

• Data Acquisition and Analysis

LB-2Bioreactor

References: Ott et al (2010) Regeneration and orthotopic transplantation of abioartificial lung, Nature Medicine, Volume 16 No 8

• Filtration Coefficient

• pH, pO2, pCO2

• Temperature

• Lung Weight


LB-2Biorea

ctorHarvardApparatus|AdvancedSolutionsforRegenerative

Medicine

NEW LB-2 Bioreactor for Mouse andRat Lung Regeneration (continued)The LB-2 is a Complete Lung Bioreactor for all phases ofLung Regeneration

Figure 1: Perfusion decellularization of whole rat lungs. (a) Photographs of acadaveric rat lung, mounted on a decellularization apparatus allowing antegradepulmonary arterial perfusion. pa, pulmonary artery; pv, pulmonary vein; tr, trachea;RUL, right upper lobe; RML, right middle lobe; LL, left lobe. Freshly isolated lung(left), after 60 min of SDS perfusion (middle), and after 120 min of SDS perfusion(right). The lung becomes more translucent as cellular material is washed out firstfrom apical segments, then from the middle segments and finally from the basalsegments.

Figure 3: Orthotopictransplantation and invivo function. (a)Photograph of left ratchest after anteriorthoracotomy, leftpneumonectomy, andorthotopictransplantation of aregenerated left lungconstruct. Recipient leftpulmonary artery (A),left main bronchus (B)and left pulmonary vein(V) are connected toregenerated left lungpulmonary artery (a),bronchus (b) andpulmonary vein (v).White arrowheads, therecipient’s right lung(infracardiac and right lower lobe); black arrowheads, the regenerated left lungconstruct. (b) Radiograph of rat chest after left pneumonectomy and orthotopictransplanation of a regenerated left lung constuct. White arrowheads, recipient’sright lung; black arrowheads, regenerated left lung construct. (c) Results of bloodgas analyses showing decrease in arterial oxygen tension (PaO2) after leftpneumonectomy and partial recovery after orthotopic transplantation of aregenerated left lung construct. Baseline, single lung, 10 min and 30 minmeasurements were obtained with the rat intubated with the extubated andbreathing room air without support of a ventilator. Upper P values compare singlelung ventilation to 5 min, and 30 min time points after transplantation at 5 min,30 min and 6 h time points after operation. Error bars, s.d.

Phase 1: Decellularization Phase 2: Regeneration

Phase 3: Validation

Phase 4: Transplantation

Order # Product73-4213 LB-2 Basic Unit for Lung Bioreactor, Size 2

Figure 2: In vitro functional testing of regenerated lung constructs. (a)Photographs of regenerated lung contructs attached to the isolated lungapparatus) pa, pulmonary arterial cannula; pv, pulmonary venous cannula; tr,trachea) in expiration (left) and inspiration (right; RUL, right upper lobe; RML, rightmiddle lobe). (b) Bar graphs showing results of blood gas analyses of pulmonaryvenous effluent of native lung, HUVEC- and A549-seeded lung groups showed gasexchange function; pO2/FiO2 ratio and pCO2 did not differ between native andH-FLC-seeded lungs. (c) Line chart showing the dynamic pressure/volumerelationship during five respiratory cycles of native, decellularized and regeneratedlungs. Corresponding compliance values (ml/cm H2O/s are shown in boxes. (d) Bargraph comparing vital capacity of native (black), H-A549-seeded lung (gray) and H-FLC-seeded, regenerated lung (red). Error bars, s.d.

Note: Images and data courtesy of H. Ott, Massachusetts General Hospital


HPC-3


HPC-3, Hydrostatic Perfusion Chamber for Organand Tissue Decellularization

Detergent or enzyme based decellularization of tissues and organs can be acumbersome process, with multiple bottles of different solutions all leading tothe tissue through a convoluted tubing network. The HPC-3 is a practicalsolution to this problem, adding useful features and enabling standardization ofdecellularization protocols for any tissue. Three independent non-heated glassvessels are mounted on a robust vertical stand to provide the requiredhydrostatic perfusion pressure and all required tubing, in appropriate lengths, isincluded. Glass vessels and the included Teflon Lids are fully autoclavable.

Order # Product73-001020 3-Fold Hydrostatic Perfusion Chamber with Stand


Cell&

TissuePerfusion


Medicine

The NaviCyteDiffusion/UssingChamber SystemsThe “World Standard” for conducting substance transport studies acrosscultured cell monolayers or excised tissue under dynamic conditions.

• Higher Throughput Screening–Up to 24 Chambers

• Easier to Use than Other SystemsApplications Include:

Drug Delivery

Toxicology

Pharmacokinetics

Metabolic Studies

Transport Studies

Cell and Tissue Perfusion, Imaging &Assay Chambers

Ideal for live cell imagingexperiments and cellularelectrophysiology

Universal Coverslip Chamber


ImagingChambers


Flow Through Devices for Removal of Toxins,Metabolites or Signaling Products

DialysisBuffer Out

DialysisBuffer In

SampleSolution Out

SampleSolution In

SampleChamber

Membrane(1.2 in x 3.2 in)

DialysisBufferChamber

Now sterilizable, in-line dialysis system to de-toxify 3D bioreactor perfusate.Take out DMSO, salts, and cell communication signal molecules so you cankeep the 3D bioreactor perfusate in safe limits for organ regeneration

The Flow-Thru DIALYZER is a unique system for the rapid dialysis of samplevolumes from 20 µl to 100 ml. It provides a large surface area for Flow-Thru on-line dialysis with minimal sample loss. The entire dialysis unit is made ofTeflon, an inert material, and has two separate serpentine channelssuperimposed on each other and separated by a dialysis membrane. The lengthof each channel is about 700 mm. Five different chambers are available (20 µl,75 µl, 150 µl, 300 µl and 600 µl). Chambers of different volumes can also besuperimposed on each other for specific applications.

• Fast• Cost-effective• Preparation of microfluidic volumesUntil now, commercially available options for preparing microliter volumes havesuffered major drawbacks, including high back pressure, clogging, leakage andlimited surface chemistry options. LabSmith’s new CapTite cartridges offercustomizable packing and re-use, for easy sample preparation in bothprototypes and high-throughput, automated systems.

Filtration or Solid PhaseExtractionIn-Line PEEK cartridge for Toxic removal

Flow-Thru DIALYZER™ & MembranesA. Regenerated Cellulose MEMBRANES:1k Da MWCO2k Da MWCO5k Da MWCO10k Da MWCO25k Da MWCO50k Da MWCOB. Cellulose Acetate MEMBRANES:100 Da MWCO500 Da MWCO1k Da MWCO2k Da MWCO5k Da MWCO10k Da MWCO25k Da MWCO50k Da MWCO100k Da MWCOC. Polycarbonate MEMBRANES:0.01 um0.05 um0.60 um


Phase4:Introd


Medicine

Introduction to Phase 4Tools, Equipment & SystemsMonitoring and recording of Physiological and Metabolic parameters, or BIOSENSING, is critical in understanding the viability oforganisms, organs, tissues and cells and is the ultimate determinant of organ validation for transplant.

Harvard Apparatus has probes and systems for more physiological and metabolic measurements than anyone else.We have tried to list all of our BIOSENSORS and in the following pages, we have noted some of the newest.

For more details or for a specific request, please contact our technical specialists at [email protected].

Cells1. Microscopic Imaging &Perfusion Chambers

2. Microscopic EnvironmentalControls

3. Electrophysiology Products4. Cell Condition Monitors

Tissues5. Isolated Tissue & PerfusionBaths

Organs6. Lung Regeneration Bioreactor7. 3D Bioreactors – HollowOrgans

Sensors8. Ion SensorsAmmonia, Calcium, Chloride,Ethanol, Glucose, Lactate,Nitrate, Nitric Oxide, Nitrite,Peroxide, Potassium, Sodium,Urea

9. Flow10. Temperature11. Pressure12. Force13. O2; CO2

Cell, Tissue, Organ & Regenerative SystemsCondition Monitoring Tools

Capnograph

Physiological Assessement &ConditioningMonitoringPHASE 4:


Biosensing


A Range of Harvard Apparatus BIOSENSINGProbes are Available for Virtually Any Metabolic orPhysiological Measurement

Application Areas• Respiratory Mechanics• Cardiovascular Physiology• Absorption• Metabolism• Excretion• Nutrition• Electrophysiology• Biopotentials• Animal Behavior• Dosing

Measurement Probes• Blood Pressure Transducers• Force Transducers• Differential Air Pressure Transducers• Flow Probes• Capnographs• Pulse Oxymeters• Temperature Monitors• Thermocouples• ECG Electrodes• Monophasic Action Potential Electrodes• Weight Transducers• Electrical Stimulation Electrodes• Imaging devices• Blood Gas Probes, pH, O2, CO2

• Ion & Compound Sensors- Ammonia- Calcium- Chloride- Ethanol- Glucose- Lactate- Nitrate- Nitric Oxide- Nitrite- pH- Peroxide- Potassium- Sodium- Urea

• Infrared Motion Detectors

Spill Sensor Mats withConnecting Cable

Control Unit

Battery Powered SpillSensor System• Protect your microscope• Audible alarm• TTL cutoff circuitry• Compatible with upright and inverted

microscopes• Easy set up and installation• Complete system

• Force Measurement

• Digital Microscopy

• Automated Blood Gasand TemperatureControl

• Air Pressure

• pCO2, pO2, pH

• Nitrate & Nitrite Ions

• Oxygen levels inbulk fluids


Optica

lDetection


Medicine

NEW Optical Detection of pH, O2 and CO2 areIdeal for Bioreactor Use

Optical Biosensors• Completely Sterilizable sensors withstand CIP

(Clean-In-Place), SIP (Steam-In-Place), gamma,and autoclave conditions.

• USP Class VI-certified sensors are shipped pre-calibrated although the system allows the userto perform a simple standardization procedure.

Now pH, O2 and CO2 can be measured easily in disposable systems using newiDots. iDots are welded into the wall of the bioprocess container, are pre-calibrated, can be gamma sterilized and are made of all USP Class VI materials.iDots are readily adaptable to your single-use application using Polestar’s DSPseries optical monitors.

Flow-cell probe is a zero dead-volume sensor that can be up with DSPseries Optical Process Monitors for continuous measurement of DO or pH.Pre-calibrated DO or pH fluorescent sensing films deposited along the innersurface of the quartz flow-cell body enabling optical interrogation through thetransparent wall of the flow-cell via standard fiber optic cable. A simplere-useable clamping device with integral fiber optic connector is provided tofacilitate mounting the device. Flexible silicone tubing on each end of the flow-cell enables quick connection to external tubing or adapters such as Luer-Lockfittings. All materials used in the construction of the flow-cell sensors are USPClass VI certified and can withstand autoclave or gamma sterilization.

Puncture probe oxygen sensors are configured for measurements of oxygen inapplications such as package integrity testing, small volume samples and softtissue monitoring. The probe is constructed from a small diameter fiber opticcapped with a thin layer of oxygen sensing chemistry fixed within the lumen ofa stainless steel hypodermic needle. Coring and non-coring designs areavailable in sizes ranging from 14 gauge to 24 gauge. Responses times (t90) forPolestar’s puncture probes are <10 seconds for measurements of gas phaseoxygen and <20 seconds for dissolved oxygen.

Designed to operate with oxygen, pH, and CO2 sensors, the DSP SeriesMonitors are the core of any process monitoring system. The DSP Seriestransmitters include automatic temperature and pressure compensation as willas a proprietary AGC (automatic gain control) to optimize readings andmaximize sensor lifespan. The NEMA 12/4X IP 65 enclosure has water-tightcordgrips for brining in process temperature and pressure data via 4-20 mAsignals, a simple and intuitive menu, and a high visibility VFD display. Capableof logging collected data in real time to virtually any data acquisition system.

Peel and Stick Sensors, iDots

Puncure Probe

DSP MonitoringUnit

Flow Cell

BagIntegratedSensors


PerfusionSolutionMonitor


Universal Perfusion Solution Monitor: provenelectrochemical detection of pH, pO2, pCO2 inhydrodynamic systems

Universal PerfusionSolution Monitor• Rugged construction• Noise free design, no cross talk• High sensitivity• Good linearityFor studies involving isolated or in situ perfused organs (heart, lung, liver,kidney) it is often important to continuously monitor pO2, pCO2 and/or pH of theperfusate or effluate. Which parameter is measured depends on the organ usedand on the type of experiment conducted.

The universal perfusion solution monitor permits precise continuous ordiscontinuous measurement in liquid media of these three key parameters pO2,pCO2 and pH. The electrodes are all side stream flow-through electrodes andrequire a pulsation-free roller pump to deliver constant flow of perfusatethrough the electrode at flow rates in the range from 0.5 to 2 ml/min. Thesolution is pulled through each of the electrodes in series. The pH sensorrequires an external reference electrode (both the pO2 and pCO2 containinternal reference electrodes). A solid state leak free reference system is used.Because of the high impedance of these sensors, screening or shielding of themeasuring circuit is required to guard against electrostatic discharges andother electrical disturbances. The shielding case also provides a convenientpoint of attachment to the multi-electrode mounting plate. Sensors areavailable individually or configured as a complete package with suitableamplifiers and Data Aquisition.

PH, pO2, & pCO2 System Detail of pH, pO2 & pCO2Sensor System

Detail of pO2 Sensor


Microscop

yHarvardApparatus|AdvancedSolutionsforRegenerative

Medicine

Guide for Advanced Metabolic and PhysiologicalEvaluation of Organisms, Organs, Tissues & Cellsusing Infrared Contrast Imaging (IRCI)

Physiological Fluidics Monitoring

Vascularization

Disease Monitoring with EnhancedVisualization


Microscopy


Guide for Advanced Metabolic and PhysiologicalEvaluation of Organisms, Organs, Tissues & Cellsusing Infrared Contrast Imaging (IRCI) (continued)Know More About Your Experiment with IRCIHarvard Apparatus has utilized infrared imaging in a way that not only provides temperature imaging but also produces high contrast images in biological andmedical applications. This new technique uses the camera’s ability to detect IR emmisivity from objects as a function of their surface texture, composition,temperature, shape and more. This new utilization of infrared imaging has been called IRCI - Infrared Contrast Imaging. This powerful technology can visualize,with high resolution, experimental conditions based on tissue vascularization, structure or metabolic state.

• Site evaluations for viable or necrotic tissue• More clearly reveal morphological features of biological substrates• Track injection flow paths in real time to evaluate injection quality

Accurate Noncontact Infrared Measurement For VisualizingPhysiological, Metabolic and Morphological features with IRCITo assure accurate noncontact infrared temperature measurement keep in mind the following:

• Distance to Target (Spot) Ratio• Field of View• Environmental Conditions• Ambient Temperatures• Emissivity

Distance to Target RatioThe distance controls the energy hitting the detector, the closer the camera the more signal being picked up.

Field-of-viewThe array must have the correct lens in front of it to get as much illumination of the emitted light onto as many pixels as possible. When choosing a system the lensshould be selected to give you the proper working distance and the magnification to display the area of interest on as much of the array as possible

Environmental ConditionsWatch for environmental conditions in the working area. Steam, dust, smoke, etc., can prevent accurate measurement by obstructing the unit's optics. Dew point orthe moisture in the air can cause blurring of the image also if you have glass containers or certain plastics they will absorb all the IR energy so they are nottransparent to the IR and you will not be able to see through these. If this happens replace the material with a IR transparent material if possible.

Ambient Temperatures (the surrounding temperature)If the thermometer is exposed to abrupt ambient temperature differences of 20 degrees or more, allow it to adjust to the new ambient temperature for at least 20minutes. The camera fixed-mounted sensors are specified for performance within certain ambient temperature ranges. For high ambient temperatures, somecamera’s offers air cooling and water cooling options as well as accessories, such as Thermojacket.

EmissivityEmissivity is the measure of an object's ability to emit infrared energy. Emitted energy indicates the temperature of the object. Emissivity can have a value from 0(shiny mirror) to 1.0 (blackbody).

Emissivity Differences Create Contrast with IRCIIRCI has the ability to enhance contrast of objects based on their emmissivity. There are many features of many different types of samples which may be revealed byIRCI, making it a particularly useful tool. Some of these features are:

• Surface Texture• Sample Thickness• Temperature• Chemical Composition• Depth Profile


Microscop

yHarvardApparatus|AdvancedSolutionsforRegenerative

Medicine

Guide for Advanced Metabolic and PhysiologicalEvaluation of Organisms, Organs, Tissues & Cellsusing Infrared Contrast Imaging (IRCI) (continued)Application Example: IRCI for Evaluation of Stem Cell Injection Site PhysiologyInfrared contrast imaging (IRCI) enables clear visizualization of physiological and structural features of living tissue which are otherwise very difficult to detect.This is demonstrated in a rodent model where cardiac infarct was induced via ligation of the left anterior descending coronary artery (LAD).

Areas of advanced cardiac infarct generally display a faint and localized “paleness” at the epicenter of the infarct, where myocardial injury is greatest and mostpronounced. The images below show, widespread changes in detected emmissivity on the myocardial surface after LAD ligation. What may be partially visibleto an experienced observer using the naked eye, is now readily apparent to even the uninitiated user of IRCI technology. As a result, this technique enablesroutine procedures to be done without extensive training and with greater accuracy.

Handheld Digital Microscope with IR illumination• Pocket sized 3.5" LCD display/image capture

• Real time image or video

• Storage and playback

• Up to 200X variable magnification

• Built-in 8-Always-On white LEDs lighting (Option near UV light)

• Microscope Resolution 628 x 586

• LCD Screen Resolution 320 x 240

Rat Orientation

i40 / i50 / i60• Economical IR camera for monitoring temperature

Small, Compact and PowerfulThe Harvard Apparatus T-Series of portable infrared camerastakes infrared camera ergonomics, weight and ease-of-use to anew level. Usability is key

Ideal for visualizing changes in vascularization or surfacefeatures of tissue and organs with otherwise low contrast.

IR Microscope• Imaging of 100 micron area at 3 micron resolution

Time Zero to Infarct 30 Seconds After Infarct One Minute After Infarct

HeartInfarctArea Heart

InfarctArea

Heart

InfarctArea

Digital Microscopy 0-500X with IntegralIllumination: Small, Powerful and Economical

AM2011• Handheld Microscope

• 0.3M/Resolution 640x480

• USB 2.0 Output

• 10~200X Various Magnification

• 1/4" Color CMOS

• Built-in 4 white LED’s

AM311SAM311ST(Micro Touch)• Handheld Microscope


• USB 2.0 Output


• LED on/off controlled by software

• AM311ST withTrigger Button

AMH-RUT• Handheld Iris Microscope

• 1.3 MP

• USB 2.0 Output

• Resolution 1280x1024

• USB 2.0


• 10x -20x Various Magnification

AMH-N5UT• Handheld Iris Microscope

• 1.3 MP

• Resolution 1280x1024

• USB 2.0


• 500X fixed Magnification

AM412N AV/TV outputAM412NT LED On/OffAM412NZT PolarizeAM412NTL Enhanced WDAM412MNT Aluminium AlloyAM412MNTL Aluminium Alloy• Handheld Microscope


• AV (Video)/TV Output


AM313/313T• Handheld Microscope


• USB 2.0 Output


• Measurement feature include (*)


AM311H EarscopeAMH-EAN AV/TV outputAMH-EUT-V3 Earscope 1.3MP• Handheld Earscope

• 0.3 MP / 1.3 MP

• USB 2.0 Output / or TV

• 10~50X / 55X~90X Magnification

• Built-in 4/6 white LED’

AMB-DT-U1 USB 5X~37XAMB-DT-U2 USB 10X~92XAMB-DT-T1 AV/TV PolarizerAMB-DT-T3 AV/TV 10X~92X• Handheld Dental Scope

• 0.3 MP (TV) 1.3 MP (USB)

• USB 2.0 Output / or TV

• Various Magnification

• Built-in 8 white LED’AMK4012-C200• Handheld Digital Microscope

• Pocket sized 3.5" LCD display/image capture

• Real time image or video Storage and playback

• Up to 200X variable magnification

• Built-in 8-Always-On white LEDs lighting (Option near UV light)

• Microscope Resolution 628x586

• LCD Screen Resolution 320x240


Microscopy



ReferencesHarvardApparatus|AdvancedSolutionsforRegenerative

Medicine

ReferencesOur extensive references cover all areas ofinterest for Regenerative Medicine

Sample Research Publications:1. Spontaneous hair cell regeneration in the mouse utricle following gentamicin ototoxicity ..BIOREACTOR K Kawamoto, M Izumikawa, LA Beyer, GM … - Hearing Research, 2009 - Elsevier... if missing haircells can be replaced by a regenerative treatment ... Infusion speed was controlled by a syringe pump (Harvard Apparatus Inc., Holliston, MA) at the ...

2. Regenerative medicine bioprocessing: concentration and behaviour of adherent cell …BIOREACTOR BJH Zoro, S Owen, RAL Drake, C Mason, M … - Biotechnology and Bioengineering, 2009 -interscience.wiley.com ... Received March 27, 2009; Accepted April 3, 2009 ... for primary cells typical in regenerative medicine. ... vertically onto a syringe Pump (PHD2000, Harvard Apparatus...

3. Towards microfabricated biohybrid artificial lung modules for chronic respiratory support…BIOREACTOR /Materials KA Burgess, HH Hu, WR Wagner, WJ … - Biomedical Microdevices, 2009 - Springer...215 McGowan Institute for Regenerative Medicine, University ... Biomed Microdevices (2009) 11:117–127 121 ... PHD 2000 syringe pump (Harvard Apparatus, Holliston, MA ...

4. An automated system for delivery of an unstable transcription factor to hematopoietic stem …BIOREACTOR E Csaszar, G Gavigan, M Ungrin, C Therien, … - Biotechnology and Bioengineering, 2009 -interscience.wiley.com... Ontario, Canada 7 McEwen Centre for Regenerative Medicine, University ... ß 2009 Wiley Periodicals, Inc ... a Model 33 Twin Syringe Pump (#553333, Harvard Apparatus, St ...

5. Three-Dimensional Gel Bioreactor for Assessment of Cardiomyocyte Induction in Skeletal Muscle–Derived Stem Cell… BIOREACTOR KC Clause, JP Tinney, LJ Liu, B … - … Engineering Part C: …,2010 - liebertonline.com... 16 Issue 3: May 26, 2010 Online Ahead of Print: February 26, 2010 Online Ahead ... Cell SortingAnalysis of Unsorted Muscle-Derived Stem Cells and Muscle-Derived Stem Cell-3DGB. ... 1 Hz,4ms, 50–100 V, rectangular pulses) using a stimulator (Harvard Apparatus, Holliston, MA ...

6. BMI1 sustains human glioblastoma multiforme stem cell renewal…STEM CELL cjb.net [HTML]M Abdouh, S Facchino, W Chatoo, V … - Journal of …, 2009 - neuro.cjb.net... This work revealed that PcGproteins are required in human GBM to sustain cancer-initiatingstem cell renewal. Materials and Methods. ... The scalp was closed with wound clips (Harvard Apparatus). Animals were followed daily for development ofneurological deficits. ...

7. In vivo multimodal imaging of stem cell transplantation in a rodent model of Parkinson's disease…STEM CELL J Jackson, C Chapon, W Jones, E Hirani, A … - Journal of neuroscience …, 2009 - Elsevier...6-Hydroxydopamine (6-OHDA) was injected into the right striatum at a dose of 16 g, in a volume of 4 L at a rate of 1 L/min, using a micropipettor (Harvard Apparatus, Edenbridge, UK). ...Post-implantation stem celllocalisation was assessed using high-resolution MR images. ...

8. Effects of ischemic preconditioning on regenerative capacity of hepatocyte in the …STEM CELL A Bedirli, M Kerem, H Pasaoglu, O Erdem, E … - Journal of Surgical Research, 2005 - Elsevier... model haveshown that initiation of the regenerative response depends ... and the common bile duct with a microvascular clamp (Harvard Apparatus, Inc., Hollinston ...

9. Development of microfluidics as endothelial progenitor cell capture technology for …STEM CELL BD Plouffe, T Kniazeva, JE Mayer Jr, SK … - The FASEB Journal, 2009 - FASEB... using a Harvard ApparatusPHD 2000 syringe pump (Harvard Apparatus, .23 October 2009 ... EPCs are a potential tool in regenerative medicine and likely ...

10. Substantial detrusor overactivity in conscious spontaneously hypertensive rats with …STEM CELL LH Jin, KE Andersson, YH Kwon - Scandinavian Journal of Urology and Nephrology, 2009 -informaworld.com... 2 Wake Forest Institute for Regenerative Medicine, Wake ... ISSN 1651-2065 online # 2009 Informa UK ... a micro- injection pump (PHD22/2000 pump; Harvard Apparatus). ...

11. Tbx3 improves the germ-line competency of induced pluripotent stem cells..STEM CELLS/ Electroporation J Han, P Yuan, H Yang, J Zhang, BS Soh, P Li, SL Lim, … - Nature, 2010 - nature.com... Received14 February 2009; Accepted 9 December 2009; Published online 7 February 2010. ...analyses, Y. Loon Lee, P. Gaughwin and colleagues from the Stem Cell and Developmental ... Cell fusion by Electro cell manipulator (ECM2001, BTX Harvard Apparatus) and incubated ...

12. Pilot study to investigate the possibility of cytogenetic and physiological changes in bio- …SCAFFOLD MATERIALS/ electrospinning H Kempski, N Austin, A Roe, S Chatters, SN … - Regen. Med., 2008- Future Medicine... 3 s -1 (PHD 4400, HARVARD Apparatus Ltd., Edenbridge ... Materials for Tissue Engineering & Regenerative Medicine2,158 ... (2009) Bio-electrospraying embryonic stem cells ...

13. In vivo bone tissue engineering using mesenchymal stem cells on a novel electrospun …SCAFFOLD MATERIALS/ electrospinning M Shin, H Yoshimoto, JP Vacanti - Tissue engineering, 2004 -liebertonline.com... Q 5 0.1 mL/min, PHD 2000 syringe pump; Harvard Apparatus, Holliston, MA ... 2009. Nanostructured polymer scaffolds for tissue engineering and regenerative medicine ...

14. Silk fibroin microtubes for blood vessel engineering…SCAFFOLD MATERIALS nih.gov M Lovett, C Cannizzaro, L Daheron, B … - Biomaterials, 2007 - Elsevier... c Center for Regenerative Medicine,Massachusetts General Hospital, Harvard Medical School ... the bioreactor using a syringe pump (Harvard Apparatus, Holliston, MA ...

15. Quantitative Analysis of Neural Stem Cell Migration and Tracer Clearance in the Rat Brain by MRI…STEM CELL JA Flexman, DJ Cross, LN Tran, T Sasaki, Y Kim, S … - Molecular Imaging and … -Springer... DOI: 10.1007/s11307-010-0311-3 Mol Imaging Biol (2010) ... morphologically transplanted cell migration in the rat brain and parametrically estimate neural stem cell migration speed. ... the skull) using apicoliter syringe pump (Pico Plus Syringe Pump; Harvard Apparatus; Holliston ...

16. Therapeutic effect of genetically engineered mesenchymal stem cells in rat experimental leptomeningeal glioma model. STEM CELL C Gu, S Li, T Tokuyama, N Yokota, H Namba - Cancer letters, 2009 -Elsevier... Mesencult ® murine mesenchymal stem cell medium) after aspiration by 5 ml syringe connected with 23 G needle ... the subarachnoid space by a 50 µl microsyringe (Hamilton Company, Reno, NV) connectedwith a 27 G needle and a microinjector (Harvard Apparatus, Inc., South ...

16. Magnetosonoporation: Instant magnetic labeling of stem cells… STEM CELL B Qiu, D Xie, P Walczak, X Li, J Ruiz- … - Magnetic…, 2010 - interscience.wiley.com... VC 2010 Wiley-Liss, Inc ... basal medium(Stemcells Inc., Vancouver, Canada), adding NeuroCult supple- ments for neural stem cell differentiation and ... Using a nanoinjector (Harvard Apparatus, Holliston, MA), the left brain hemispheres were locally implantedwith approximately 8 ...

17. A seeding device for tissue engineered tubular structures BIOREACTOR L Soletti, A Nieponice, J Guan, JJ Stankus, WR Wagner … - Biomaterials, 2006 - Elsevier... The tees were connected to a precisionsyringe pump (Harvard Apparatus Inc.,Holliston, MA, USA) outside the chamber by means of hydraulic rotating joints (DeublinCo., Waukegan, IL, USA) and polyvinyl chloride (PVC) tubing. ...

APPLICATION GUIDES:• BILAYER WORKSTATION• BOLUS INJECTIONS• CELL INFUSION• ELECTRODE• CULTURE DISH FEEDING• DRUG INJECTIONS• ELECTROSPINNING SCALFOLDS

• IMAGING• ISOLATED HEART SYSTEM• NANOFLUIDICS• STEM CELL INJECTIONS• STEM CELL COLLECTION AND HARVESTING• USSING CHAMBER• VENTILATING ANIMALS


References


References

18. A novel culture system shows that stem cells can be grown in 3D and under physiologic pulsatile conditions for tissue engineering of vascular grafts 171.65.102.190….BIOREACTOR [PDF]O Abilez,P Benharash, M Mehrotra, E … - Journal of Surgical…, 2006 - Elsevier... system that would allow future testing of mechanical, biochemical, and thermal stimuli on stem cell differentiation into ... (B) Photo of thebioreactor (left) and ... The pulsatile pump was a Harvard Apparatus Model 1405 (Harvard Apparatus, Holliston, MA) modified for computer control...

19. … Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and Prevascularization…BIOREACTOR M Lovett, DRockwood, A Baryshyan, DL … - … Engineering Part C: …, 2010 - liebertonline.com... A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation,and ... Medford, MA 02155. E-mail: Received: April 19, 2010 Accepted: June 1, 2010. ... syringe pump (Remote PHD Push/Pull Programmable Pump; Harvard Apparatus, Holliston, MA ...

20. A Three-Dimensional Gel Bioreactor for Assessment of Cardiomyocyte Induction in Skeletal Muscle–Derived Stem Cells BIOREACTOR KC Clause, JP Tinney, LJ Liu, B … - … Engineering Part C: …,2010 - liebertonline.com... Activated Cell Sorting Analysis of Unsorted Muscle-Derived Stem Cells and Muscle-Derived Stem Cell-3DGB. MDSC-3D collagen gel bioreactor (MDSC-3DGB) construction ... 1 Hz, 4 ms, 50–100V, rectangular pulses) using a stimulator (Harvard Apparatus, Holliston, MA). ...

21. Fabrication and Characterization of Prosurvival Growth Factor Releasing, Anisotropic Scaffolds for Enhanced Mesenchymal Stem Cell Survival/Growth and Orientation …SCAFFOLD MATERIALF Wang, Z Li, K Tamama, CK Sen, J Guan - Biomacromolecules, 2009 - ACS Publications... It has been shown to enhance survival of many cell types including smooth muscle cell, cardiomyocyte, and mesenchymal stemcell.(21-23) Besides, IGF-1 ... The gelatin/BSA/IGF-1 solution was fed at a rate of 0.2 mL/h by syringe pump A (Harvard Apparatus) into the inner tubing. ...

22. Fabrication and Characterization of Prosurvival Growth Factor Releasing, Anisotropic Scaffolds for Enhanced Mesenchymal Stem Cell Survival/Growth and Orientation …SCAFFOLDMATERIALS F Wang, Z Li, K Tamama, CK Sen, J Guan - Biomacromolecules, 2009 - ACS Publications... It has been shown to enhance survival of many cell types including smooth muscle cell, cardiomyocyte, andmesenchymal stem cell.(21-23) Besides, IGF-1 ... The gelatin/BSA/IGF-1 solution was fed at a rate of 0.2 mL/h by syringe pump A (Harvard Apparatus) into the inner tubing. ...

23. Porous nanocrystalline silicon membranes as highly permeable and molecularly thin substrates for cell culture …SCAFFOLD MATERIAL AA Agrawal, BJ Nehilla, KV Reisig, TR Gaborski, DZ … -Biomaterials, 2010 - Elsevier... Received 8 March 2010; accepted 16 March 2010. Available online 15 April 2010. Abstract. ...An open perfusion microincubator (Harvard Apparatus) was used to maintain cells at 37 °C andmineral oil was floated on top of the media to minimize evaporation. ...

24. A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning… SCAFFOLD MATERIAL nih.gov [HTML]F Teulé, AR Cooper, WA Furin, D Bittencourt, EL … - Natureprotocols, 2009 - nature.com... Bacteria have quicker generation times and do not require a very sophisticated laboratory setup,thus reducing time and expenses. Additionally, the production can be easily scaled-up usingbioreactors. ... no. P-259); Harvard Pump 11 plus single syringe (Harvard Apparatus, cat. ...

25. Pressure driven spinning: A multifaceted approach for preparing nanoscaled functionalized fibers, scaffolds, and membranes with advanced materials …SCAFFOLD MATERIAL nisco.ch [PDF]SNJayasinghe, N Suter - Biomicrofluidics, 2010 - link.aip.org... Biomicrofluidics 4, 014106 (2010)]. ... glass microslides, while for scanning electron microscopy (SEM) and transmission electron microscopy (TEM), fibers werecollected ... at the lowest possible flow rate allowed by our present syringe pump, (PHD4400, Harvard Apparatus Ltd., Kent ...

26. Characterization and tensile strength of HPC-PEO composite fibers produced by electrospinning …SCAFFOLD MATERIAL L Francis, A Balakrishnan, KP Sanosh, E Marsano - Materials Letters, 2010 -Elsevier ... strength of HPC-PEO composite fibers produced by electrospinning, Materials Letters (2010), doi: 10.1016/j ... PEO, Sigma Aldrich, Mw = 10,000) were used as starting precursors to fabricate nano fibers. ... of250 m and was mounted on a syringe pump (Harvard Apparatus serial n A ...

27. Tbx3 improves the germ-line competency of induced pluripotent stem cells..STEM CELL/ Electroporation J Han, P Yuan, H Yang, J Zhang, BS Soh, P Li, SL Lim, … - Nature, 2010 - nature.com ... karyotypeanalyses, Y. Loon Lee, P. Gaughwin and colleagues from the Stem Cell and Developmental ... h, inactivated feeder cells and fresh media were added, and the culture was then ...2-cell fusion by Electro cell manipulator(ECM 2001, BTX Harvard Apparatus) and incubated ...

28. Osteochondral Interface Tissue Engineering Using Macroscopic Gradients of Bioactive Signals …STEM CELLS NH Dormer, M Singh, L Wang, CJ Berkland, MS … - Annals of biomedical…, 2010 -Springer ... 0090-6964/10/0600-2167/0 © 2010 Biomedical Engineering Society ... There is a growing interest in hUCMSCs as a mesenchymal stem cell source, as they have been ... in a controlled manner usingprogrammable syringe pumps (PHD 22/2000, Harvard Apparatus, Inc., Holliston ...

29. Engineered Early Embryonic Cardiac Tissue Increases Cardiomyocyte Proliferation by Cyclic Mechanical Stretch via p38-MAP Kinase Phosphorylation …BIOREACTOR nih.gov [HTML]KC Clause,JP Tinney, LJ Liu, BB Keller, … - … Engineering Part A, 2009 - liebertonline.com ... 1 Hz, 4 ms, and 70–100 V) using a stimulator (Harvard Apparatus, Holliston, MA). ... Pasumarthi, KB, Soonpaa, MH, and Field, LJ Myocyteand myogenic stem cell transplantation in ... A Three-Dimensional Gel Bioreactor for Assessment of Cardiomyocyte Induction in Skeletal Muscle ...

30. Dynamic Seeding of Perfusing Human Umbilical Vein Endothelial Cells (HUVECs) onto Dual-Function Cell Adhesion Ligands: Arg-Gly-Asp (RGD)− Streptavidin and …BIOREACTOR CC Anamelechi,EC Clermont, MT Novak, WM … - Langmuir, 2009 - ACS Publications... combined a dynamic seeding system with a bioreactor proliferation phase to increase cellular adhesion and proliferation for three-dimensionalpolymer ... The dynamic seeding system consisted of a 10 mL syringe mounted on a Harvard Apparatus pump, infusing cells onto Teflon ...

31. Dynamic Seeding of Perfusing Human Umbilical Vein Endothelial Cells (HUVECs) onto Dual-Function Cell Adhesion Ligands: Arg-Gly-Asp (RGD)− Streptavidin and …BIOREACTOR CC Anamelechi,EC Clermont, MT Novak, WM … - Langmuir, 2009 - ACS Publications... combined a dynamic seeding system with a bioreactor proliferation phase to increase cellular adhesion and proliferation for three-dimensionalpolymer ... The dynamic seeding system consisted of a 10 mL syringe mounted on a Harvard Apparatus pump, infusing cells onto Teflon ...

32. Regeneration and orthotopic transplantation of a bioartificial lung-BIOREACTOR HC Ott, B Clippinger, C Conrad, C Schuetz, I … - Nature Medicine, 2010 - nature.com ... Received 22 December 2009 Accepted04 July 2010 Published online 13 July 2010 ... black arrowheads), axial (gray arrowheads) and septal (black arrows) elastic fibers in decellularized ...trachea and was placed in a sterile, water-jacketed organ chamber(Harvard Apparatus). ...

33. Liver-specific functional studies in a microfluidic array of primary mammalian hepatocytes sbi.org …BIOREACTOR [PDF]BJ Kane, MJ Zinner, ML Yarmush, M Toner - Anal. Chem, 2006... 1. A low-magnification micrograph of an entire well is shown in Figure 5. Also on post-seeding day 1, the cell culture medium was ... These syringes were loaded into a programmable pump capable of supporting 10 individualsyringes (PHD2000, Harvard Apparatus, Holliston, MA ... Cited by 53 - Related articles - All 9 versions

34. … Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and Prevascularization …BIOREACTORS M Lovett, DRockwood, A Baryshyan, DL … - … Engineering Part C: …, 2010 - liebertonline.com... Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and ... thecollagen gel using a programmable syringe pump (Remote PHD Push/Pull Programmable Pump; Harvard Apparatus, Holliston, MA ...

35. Simple Modular Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and … BIOREACTOR M Lovett, DRockwood, A Baryshyan, DL … - … Engineering Part C: …, 2010 - liebertonline.com... tube embedded within the collagen gel using a programmable syringe pump (Remote PHD Push/Pull Programmable Pump; HarvardApparatus, Holliston, MA). For oxygen measurements,a custom housing for the bioreactor and oxygen measurement probe was ...

36. Cardiac tissue engineering using perfusion bioreactor systems …BIOREACTOR nih.gov [HTML]M Radisic, A Marsano, R Maidhof, Y Wang, G … - Nature protocols, 2008 - nature.com... only within theconstruct, that is, at the construct outlet oxygen concentration stops varying as a function of bioreactor length ... 97063A134), coated with PTFE by Microsurfaces; Stereomicroscope; Sterilization pouches; Syringe pump(Push/Pull; Harvard Apparatus or WPI Instruments ...

37. Simple Modular Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and … BIOREACTOR M Lovett, DRockwood, A Baryshyan, DL … - … Engineering Part C: …, 2010 - liebertonline.com... Medford, MA 02155. E-mail: Received: April 19, 2010 Accepted: June 1, 2010. ... 4 µL/min) of the silk tube embedded within thecollagen gel using a programmable syringe pump (Remote PHD Push/Pull Programmable Pump; Harvard Apparatus, Holliston, MA). ...

38. Distribution of NTPDase5 and NTPDase6 and the regulation of P2Y receptor signalling in the rat cochlea nih.gov …BIOREACTOR [HTML]MG O'Keeffe, PR Thorne, GD Housley, SC Robson, … -Purinergic…, 2010 - Springer... Purinergic Signalling (2010) 6:249–261 251 Page 4. ... Plastic tubing was placed inside the inlet and outlet holes and both tubes were connected to separate Hamilton syringes secured on apush–pull syringe pump (Harvard Apparatus, USA). ...

39. Liver-specific functional studies in a microfluidic array of primary mammalian hepatocytes sbi.org…BIOREACTOR [PDF]BJ Kane, MJ Zinner, ML Yarmush, M Toner - Anal. Chem, 2006 -These syringeswere loaded into a programmable pump capable of supporting 10 individual syringes (PHD2000, Harvard Apparatus, Holliston, MA). ... demonstrated that cocultured primary hepatocytes and fibroblasts in a flat platebioreactor were not adversely effected by wall ...

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UNITED STATESHarvard Apparatus84 October Hill RoadHolliston, Massachusetts 01746, USAphone 508.893.8999toll free 800.272.2775 (USA Only)

fax 508.429.5732e-mail [email protected] www.harvardapparatus.com

Warner Instruments1125 Dixwell AvenueHamden, Connecticut 06514, USAphone 203.776.0664toll free 800.599.4203 (USA Only)

fax 203.776.1278e-mail [email protected] www.warneronline.com

Coulbourn InstrumentsAttn: Sales Department5583 Roosevelt StreetWhitehall, Pennsylvania 18052, USAphone 610.395.3771fax 610.391.1333e-mail [email protected] www.coulbourn.com

CANADAHarvard Apparatus CanadaAttn: Sales Department6010 Vanden AbeeleSaint-Laurent, Quebec, H4S 1R9, Canadaphone 514.335.0792

800.361.1905 (Canada only)fax 514.335.3482e-mail [email protected] www.harvardapparatus.ca

FRANCEHarvard Apparatus, S.A.R.L.Attn: Sales Department6 Avenue des AndesMiniparc – Bat. 891952 Les Ulis Cedex, Francephone 33.1.64.46.00.85Fax 33.1.64.46.94.38e-mail [email protected] www.harvardapparatus.fr

GERMANYHugo Sachs ElektronikHarvard Apparatus, GmbHGruenstrasse 1D-79232 March-Hugstetten, Germanyphone 49.7665.92000fax 49.7665.920090e-mail [email protected] www.hugo-sachs.de

SPAINPanlab, S.L.Harvard Apparatus SpainC/Energia, 11208940 Cornellà, BarcelonaSpainphone 34.934.750.697 (International Sales)phone 934.190.709 (Sales in Spain)fax 34.934.750.699e-mail [email protected] www.panlab.com

UNITED KINGDOMHarvard Apparatus, Ltd.Attn: Sales DepartmentFircroft Way, EdenbridgeKent TN8 6HE, United Kingdomphone 44.1732.864001fax 44.1732.863356e-mail [email protected] www.harvardapparatus.co.uk

Harvard Apparatus believes in providing the best technical support. Our support specialists can assist you with pre-sales recommendations and in configuringcomplex infusion or anesthesia set-ups, selecting the appropriate surgical equipment, or selecting the best pump for Microdialysis. After purchase we assist inmaking sure your system performs your tasks to your satisfaction.

Web Services for you• Easy to find what you want: Search box or product buttons• Full specifications and descriptions• On-line operator manuals downloadable as PDF’s• 1000’s of on-line publications downloadable as PDF’s• A wide range of physiological, anatomical, biological,chemical information sources for you to downloadable

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Note: For Research Use Only. Not for use in humans unless proper investigational device regulations have been followed.

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