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Report of the 2007 International Ring Trial for infectious bursal disease virus (IBDV) antibody detection in serum December 2007 Dr J.J. de Wit H.W.A. van de Sande G.J. Buter Gezondheidsdienst voor Dieren B.V. (Animal Health Service Ltd) P.O. Box 9 7400 AA Deventer the Netherlands !"#"$$%!# ! "#$ ! #%&’( !))*))! +& ,() ,() - *))! +& . ///))! +& (’ 012 3’ ,’(42’’+

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Page 1: Report of the 2007 International Ring Trial for infectious ...alfabiolperu.com/pdf/pruebas_internacionales/2007... · Serology is widely used as a diagnostic tool and for predicting

Report of the 2007 International Ring Trial for infectious bursal disease virus (IBDV)

antibody detection in serum

December 2007

Dr J.J. de Wit H.W.A. van de Sande

G.J. Buter

Gezondheidsdienst voor Dieren B.V. (Animal Health Service Ltd)

P.O. Box 9 7400 AA Deventer

the Netherlands

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Page 2: Report of the 2007 International Ring Trial for infectious ...alfabiolperu.com/pdf/pruebas_internacionales/2007... · Serology is widely used as a diagnostic tool and for predicting

2007 IBDV ring trial report page 2

Introduction Infectious Bursal Disease or Gumboro disease is caused by an highly contagious virus, which can cause mortality and/or severe immunosuppression in young chicks. Timing of vaccination against IBD is critical and related to the level of maternal antibody in young chicks. Vaccinating too early can lead to neutralization of the vaccine due to the presence of (high levels of) maternal antibody. Vaccinating too late leads to a prolonged susceptibility to field infection. Serology is widely used as a diagnostic tool and for predicting the optimal time of IBD vaccination, based on the levels of maternal antibody in young chicks at a given moment (usually day-old), the decline of maternal antibody (half life) and the potency of the vaccine. Since quantitative results are needed, virus neutralization tests (VNT) or ELISA are used, the latter being less time-consuming and more suitable for large scale testing. VNT are usually "in-house" systems using a standard IBD strain (e.g. vaccine) and frequently different conditions. Reed et al. (1) have shown the added value of a standard reference preparation. Several commercial ELISAs are available, and many are claiming high comparability with VNT, which is still considered the reference test or "gold standard" for the detection of IBD antibody. This report contains the results of the 2007 International IBDV antibody Ring Trial (proficiency test) that was organized by the R&D Laboratory of GD, the Dutch Animal Health Service, Deventer, the Netherlands. The goal of this ring trial was to determine the performance of individual laboratories for specific tests. Samples The IBDV antibody Ring Trial consisted of 8 freeze-dried sera, which were sent to each of the participants with the request to test them for antibodies against IBV using all techniques in operation at the time. The samples were identified by sample numbers (#1 - #8) only. The sample numbers #1 - #8 are mentioned in this report, but they are supplemented with extra information to indicate the origin of the sample. In the presentations of the reported results samples are sorted the order as indicated in table 1. Table 1 - Description of samples

Sample number

Origin

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All samples, except samples #6 and #4, were produced in a vaccination experiment in which 16-week-old SPF White Leghorn birds kept in Horsefall-Bauer isolators were individually vaccinated with a single dose of live IBDV vaccine according to the manufacturer’s instructions. At 7, 10 or 14 days post vaccination (d.p.v.), birds were removed for bleeding. The sera were pooled before freeze-drying. Sample #6 is a pooled serum from vaccinated broilers that had been challenged with the very virulent IBDV strain D6948. Sample #7 is a pooled serum from 1-year-old SPF layers. Participants In total, 48 different laboratories participated from 24 countries from Africa, Asia, Europe and South America in this trial. Each of the 48 laboratories was given a unique ID-code which was only disclosed to the laboratory itself. Laboratories are identified in this report by their respective ID-code. The participating laboratories are listed in table 2.

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2007 IBDV ring trial report page 3

Tests Reported results included data for AGPT (n=10), VNT (n=2) and ELISA (n=52). Several laboratories reported results of different ELISAs or different batches of the same ELISA. There were 7 ELISA brands used in this ring trial: IDEXX ELISA by 22 laboratories, BioChek by 12 laboratories, Guildhay and Synbiotics each by 4 laboratories, LDL by 3 laboratories, Hipra and an in-house ELISA each by 1 laboratory. Analyses and statistics The goal of this ring trial was to determine the performance of individual laboratories for specific tests. The report is focused on giving participants an insight in how their results compare to the results of the other laboratories. The applied statistical calculations are based on the guidelines in ISO 5725. These statistical calculations were only performed when there were at least 6 laboratories participating within one test system. We have tried to keep the results as graphical as possible and have presented the VNTI results as log2 titres and as positive/negative results. The ELISA results are presented in 2, 3 or 4 different ways: reported titre, log2 titre, z-scores and positive/negative (or suspect) result and a XY-diagram. Before the statistical calculations, a Grubbs test was performed to detect the outliers. Outliers were excluded from the calculations of the mean results of a group of laboratories or test system. Results that were reported as <x were transformed to 0.5x for the statistical calculations. Results (log2) that were reported as �x were transformed to x+1. Definitions The total mean titre of a test system is the mean of all reported titres (excluding the outliers) for all sera and laboratories together. The total mean titre of a serum is the mean of all reported titres (excluding the outliers) for that serum by all laboratories using the same test system The z-scores were calculated using the formula below (i): (i) z=(y - m) / s where z = z-score

y = measured value of laboratory X m = mean value (all laboratories, excluding the outliers) s = standard deviation SD (all laboratories, excluding the outliers)

A z-score of the titre of the serum below -2.0 or above than 2.0 means that the result is more than 2 standard deviations different from the mean result of other laboratories for that serum using the same ELISA. It is important to realize that the z-score is related to the S.D. value. When a test shows little variation between different laboratories (small S.D.) scoring a z-score of more than 2 (or less than -2.0) can be caused by a limited difference from the mean result. On the other hand, when a test shows a big difference between the results of different laboratories (high s.d.), scoring a z-score of more than 2 (or less than -2.0) will be caused by a substantial difference from the mean result. The individual z-scores of all results are presented in tables. Individual outlier results are presented in bold and red colour. The average z-score (mean of the individual z-scores for all sera reported by a laboratory for a test system) is presented in XY-diagrams and a table. When the average z-score of a laboratory is lower than -2.0 or higher + 2.0, it is noted in this table with ‡. The XY-diagrams show the difference expressed in Z-scores for the average titer of the first test run (z1) and second test run (z2) compared to the average result (titer) of all laboratories using the same test system.

The trueness of the test results of a laboratory was defied as the difference in their total mean titre or S/P value (of all sera) compared to the total mean titre (or S/P ratio) of all laboratories (after exclusion of outliers) together using the same system. When the average of the reported titres (S/P) of a laboratory was more than 1.0 log2 step (unit) lower or higher than the average titre of all laboratories together using the same test, it is mentioned in the text

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2007 IBDV ring trial report page 4

The within laboratory reproducibility (Rwithin) or precision (ISO 5725-2) of the test results of a laboratory was defied as the variance of the (absolute) differences between the test results (expressed as log2 titres) of the duplicates. When the average Rwithin of a laboratory was more than 1.0 log2 step, it is mentioned in the text. The between laboratory reproducibility (Rbetween) was defined (ISO 5725-2) as the square root of the mean variances in reported titres (or S/P ratio) for the 8 sera by all laboratories using the same test system.

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2007 IBDV ring trial report page 5

Results AGPT Table 3 shows the qualitative results for the AGPT performed by the 10 laboratories. Gallivac, 10 d.p.v. serum #1: Eight laboratories reported positive results, one negative results and one participant a pos/neg result. Bursine2, 14 d.p.v. serum #2: all 10 laboratories reported positive results. D78 serum, 10 d.p.v. serum #3: all laboratories reported positive results. D78 serum, 7 d.p.v. serum #4: One laboratory scored this sample positive and 9 laboratories reported negative results. Bursine2, 10 d.p.v. serum #5: 8 laboratories reported positive results and 2 laboratories reported negative results. Vacc-Chall serum #6: all laboratories reported positive results. SPF serum #7: all laboratories reported negative results. D78 serum, 14 d.p.v. serum #8: all laboratories reported positive results. All except one reported duplicate results were identical, showing a high Rwithin. VNT Results Tables 4a and 4b show the quantitative and qualitative results for the VNT performed by 2 laboratories. Using IBDV serotype 1 antigens, both laboratories scored the SPF sample #7 negative. All laboratories reported positive results (except for suspect results for serum #4 by one laboratory) on all sera from vaccinated or vaccinated/challenged birds. The Rwithin of laboratories 818 and 321 was 0.56 and 0.50. ELISA Results Tables 6 (a, b, c, d, e and XYdiagram), 7 (a, b, c, d, e, XYdiagram), and 8 (a,b,c and e) show the qualitative and quantitative results obtained with all ELISAs (n=52). The qualitative results were based on the cut off values/ thresholds as indicated by the respective manufacturers. It should be noted that not all ELISAs use a "suspect" range. Several laboratories used other cut-offs than indicated by the manufacturers. To be able to compare the results of the participating laboratories the interpretation of the titres was according to the cut-off as indicated by the manufacturer of the ELISA. The results of 2 ELISAs that were used more often are presented separately. IDEXX standard IBDV ELISA (Tables 6a,b,c, d, e and XY-diagram) Twenty-two laboratories reported results of the IDEXX standard ELISA (one laboratory reported 2 sets of results). Several laboratories used there own cut-offs. To be able to compare the results of the participating laboratories the interpretation of the titres in this ring trial was according to the cut-off as indicated by the manufacturer of the ELISA. Gallivac, 10 d.p.v. serum #1: twenty-two laboratories reported positive results, one laboratory pos/neg results. Bursine2, 14 d.p.v. serum #2: 21 laboratories reported positive results, one negative results and one laboratory pos/neg negative results. D78 serum, 10 d.p.v. serum #3: 22 laboratories reported positive results, one laboratory pos/neg results. D78 serum, 7 d.p.v. serum #4: 20 laboratories reported positive results, two negative results and one laboratory pos/neg negative results Bursine2, 10 d.p.v. serum #5: 22 laboratories reported positive results and one laboratory reported negative results. Vacc-Chall serum #6: all laboratories reported positive results. SPF serum #7: all laboratories reported negative results. D78 serum, 14 d.p.v. serum #8: all laboratories reported positive results. The trueness in reported ELISA titres for each of the laboratories (table 6e) varied from -2.4 till 1.0. The averages of the reported titres of 3 laboratories were more than 1 log2 step lower

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2007 IBDV ring trial report page 6

(158, -2.4; 730, -1.1) or higher (324, 1.0) than the average titre of all laboratories together. The z-scores of each laboratory for each sample are presented in table 6d. Outliers are presented in bold red. The overall results are presented in table 6e and the XY-diagram The Rwithin of the participants varied from 0.07 till 0.92 log2 step (table 7e). The Rbetween was 0.86. BioChek IBDV ELISA (Tables 7a,b,c, d, e and XY-diagram) Twelve laboratories reported results of the BioChek ELISA (one laboratory reported 3 sets of results and one laboratory 2 sets of results). Gallivac, 10 d.p.v. serum #1: all laboratories reported positive results. Bursine2, 14 d.p.v. serum #2: all laboratories reported positive results. D78 serum, 10 d.p.v. serum #3: all laboratories reported positive results. D78 serum, 7 d.p.v. serum #4: all laboratories reported positive results. Bursine2, 10 d.p.v. serum #5: all laboratories reported positive results. Vacc-Chall serum #6: all laboratories reported positive results. SPF serum #7: 13 laboratories reported negative results, one suspect results and one laboratory suspect/neg results. D78 serum, 14 d.p.v. serum #8: all laboratories reported positive results. The trueness in reported ELISA titres for each of the laboratories (table 7e) varied from -1.5 till 0.6. The averages of the reported titres of 1 laboratory was more than 1 log2 step lower (69, -1.5) than the average titre of all laboratories together. The z-scores of each laboratory for each sample are presented in table 7d. Outliers are presented in bold red. The overall results are presented in table 7e and the XY-diagram The Rwithin of the participants varied from 0.03 till 0.48 log2 step (table 7e) The Rbetween was 0.75. Other ELISAs (Tables 8a,b,c and e) The results of the Synbiotics, Guildhay, LDL, Hipra, and an in-house ELISA are listed in Tables 8a, 8b, 8c and 8e. Fifteen laboratories reported results of the BioChek ELISA. Gallivac, 10 d.p.v. serum #1: all laboratories reported positive results. Bursine2, 14 d.p.v. serum #2: all laboratories reported positive results. D78 serum, 10 d.p.v. serum #3: all laboratories reported positive results. D78 serum, 7 d.p.v. serum #4: 8 laboratories reported positive results, 5 reported negative results and one laboratory reported neg/suspect results. Bursine2, 10 d.p.v. serum #5: all laboratories reported positive results. Vacc-Chall serum #6: all laboratories reported positive results. SPF serum #7: 9 laboratories reported negative results, 3 laboratories reported positive results. One laboratory reported pos/neg results and one laboratory reported no results. D78 serum, 14 d.p.v. serum #8: all laboratories reported positive results. The Rwithin of the participants varied from 0.04 till 0.41 log2 step (table 8e).

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2007 IBDV ring trial report page 7

Discussion & Conclusions AGPT Although a variety of IBDV strains was used for the AGPTs, the overall results showed positive results on the vaccination/challenge serum and on 5 out of 6 post vaccination sera. All results for the SPF serum were negative. VNT Both VNTs were negative on the SPF serum and positive (few suspect) on all other sera. ELISA Forty-four out of 52 laboratories scored the SPF serum negative (in duplicate). Three laboratories reported positive results (duplicate) on this serum. With few exceptions all laboratories reported positive results for the field serum and post vaccination day sera. So, in general, the sensitivity of the ELISAs was high. The results show clear differences in presented titres (all ELISAs transform the result of a single well into a “titre” by using a mathematical formula) by different ELISAs.

Please note:

The objective of this ring trial is the evaluation of the laboratory competence in the testing of defined samples by two or more laboratories. Variations may be an indication of difference in assay or technique performance as well as laboratory conditions. If your results are not as expected, please contact your test kit manufacturer.

This report may not be used for commercial purposes

Acknowledgements We would like to thank all participants for their enthusiasm, their quick response and their valuable comments.

phone: (+31) (0)570 660631 fax: (+31) (0)570 660135 e-mail: [email protected] www: www.gddeventer.com

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2007 IBDV ring trial report page 8

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Participants Country Contact Clinic for Avian, Reptile and Fish Medicine University of Veterinary Medicine Austria Prof. Dr. Michael Hess / Isabella Enigl

TM Ltd Belarus Michael Posrednikov

DGZ Vlaanderen Verbond Antwerpen Belgium R. Peeters / Els Goyvaerts

Regional Veterinary Station Bulgaria Ivaylo Nankov Naydenov

School of Veterinary Medicine Universidad Nacional Laboratory of Virology Costa Rica Dr. Carlos Jimenez Sanchez

Croatian Veterinary Institute, Poultry Centre Croatia Dr. Vladimir Savic

Ministry of Agriculture, Nat. Resources & Environment, Vet. Services Cyprus Dr. Kyriacos Georgiou

National Veterinary Institute Denmark Elisabeth Holm

National Lab of quality control of Poultry production Egypt Dr. Mona M. Aly

EVIRA Finnish Food Safetey Authority Virology Finland Christine Ek-Kommonen

Synbiotics Europe SAS France Mariette Mallein

Gemeinschaftspraxis Dres. Arnold Germany I.A. Brinkmann / Herr Dr. Thorsten Arnold

Staatliches Tierartzliches Untersuchungsamt Germany Dr Alexandra Kley / Dr. J.E. Lohr

Lohmann Tierzucht GmbH Germany Dr. Hans Philipp

LVL GmbH Lebensmittel-und Veterinäruntersuchungslabor Germany Dr. Kirsten Müller

Food GMBH Jena Germany H. Pfuetzner

Labor Diagnostik Leipzig Germany Bigit Kosch / Brigitte Kaltschmidt

Institute for Veterinary Medicinal Products Hungary Dr. Edith Nagy

Kowsar Vet Laboratorium Iran Ali Baghbanzadeh

Istituto Zooprofilattico Sperimentale delle Venezie Lab. Di Virologia Aviare Italy Dr. Stefano Nardelli / Anna Toffan

Istituto Zooprofilattico della Lombardia e dell' Emilia Italy Dr. Ana Moreno

Laboratorio Tre Valli Italy Fabio Perini

Provimi Jordan Jordan Samia Samhouri /Dr Dirgham Roussan

Vet Diagnostics (M) SDN.BHD Malaysia 03-7873 7355

Veterinair Centrum Someren Afd. Laboratorium Netherlands Hetty Reinders / W.A.J. Camp

Gezondheidsdienst voor Dieren Netherlands Charlotte van Lemel

Brabants veterinair Laboratorium Netherlands Pearl van Beurden

BIOSECURITY New Zealand Inv. and Diagnostic Centre Wallaceville New Zealand Dr. Reinhold Kittelberger/ Ann Gibbons

Intevet S.A Peru Dra.Magali Salas Martinez

National Veterinary Research Institute Poultry Dis. Dept. Poland Dr. Z. Minta / Krysztof Smietanka

SLW Biolab Poland Jaroslaw Szubstarski

Avi-Vet Service Poland Jolania Bodyt

Grampian Veterinary and Food Diagnostic Service Scotland Barbara Robinson

Aviagen Limited Veterinary Laboratory Scotland Laura Leigh / Tracy Marshall

University of Pretoria, Departement of Tropical Diseases South Africa Dr. JE Crawford / Dr. S. Bisschop

MasterLab Madrid Trouw Nutrition Spain Mónica Picó / R. Amejeiras Rodriguez

SEYSA Spain Dr M.J. Martinez-Aleson

Laboratorio labdial Laborarorio de Diagnósticos y Alimentatión Spain Monica Clerigo Lera / Theresa Aranguena

Centre De Sanitat Avicola de Catalunya Spain

National Veterinary Institute Poultry Department Sweden Lena Renström

IDEXX Switserland AG Production Department Switzerland Andrea Ballagi

Veterinar Bakteriologie der Universtat Zurch Switzerland Richard Hoop

Ross breeders Anadolu SA Turkey Mehmet Uvey / Don McLeod

Guildhay Limited United Kingdom Bruce Coote / Fiona Anderson

Sci-Tech Laboratories The Crove United Kingdom Mr. R.T. Harrison

Faccenda Chicken Ltd. United Kingdom A.J. Summers / Marion Gee

Vet Lab. Sun Valley Foods ltd. United Kingdom N. Fincham

Zimvet (Pvt) (Ltd) Zimbabwe Kudzaiishe Manika

Page 9: Report of the 2007 International Ring Trial for infectious ...alfabiolperu.com/pdf/pruebas_internacionales/2007... · Serology is widely used as a diagnostic tool and for predicting

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066 +/7;� +--,� *;)7� *--,� ,)./� +0.+� ;+*� /-/� *),*� ).;;� ,;,)� -)7-� .)� ./� ,;,.� ,/0;� � /,)�$5��

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108 ,,;7� ,/7,� +).)� +--.� +0.-� ,*+,� ,/.� -;,� *+0-� *.*;� ,0.+� -0,+� *,;� );,� ,7+7� ,.-)� 7;*.79� .+;�

158 ++0� --*� ++7� +/.� +.*� -.)� *);� *),� **.� *0,� ,.+� .)0� .+� .0� ,*,� /*.� 7;*.79� .+;�

207 *70;� *./7� )7;+� )0;,� */+)� *,-)� .,.� .,)� )+;.� )*+*� *00;� *;0+� )*0� )� *;-7� *0--� 7;*.79>�7*;�

217 +)-*� +)77� **/0� *,;,� *./.� +0/)� 0-0� 0.7� )-*;� )+0;� ,)*/� +,,0� .)� /0� ,)7)� ,7;7� 7;*.79� .+;�

303 *0-7� +/..� )0./� +*07� *;--� ,;/7� 0),� )7*7� ).,+� *+))� +-,+� +0.-� 0*� *7� +.)+� ,;,*� 7;*.79"�7*;2� �+;�

324 +;;;� +0-0� *;,7� *.;.� ,.-;� ,+*;� ;-;� ./+� *)7*� )0//� -+.7� -*;0� 7� 7� -*..� ,/*7� 7;*.79� .+;�

490 +77.� */./� *+-+� **,0� +.77� +/+7� ;70� /;+� ).--� )+07� +,7.� +,-7� +.� -.� +/;-� +/;-� 7;*.79"�7*;�

500 +0.0� +7-7� *,,.� *-*)� ,*),� +;*.� /.7� .;/� *+).� )/..� ,/..� ,,*7� ,,� *+� ,*)-� +0-)� 7;*.79� .+;�

515 *);)� *7)-� ;-/� 7� *7//� **,)� ,;;� +;7� ,7)0� ))+-� +,.)� **).� )*0� )7;� +)70� */0/� 7;*.79��)0.�

604 +;0+� +;+)� *,*)� *--*� ++70� ++70� /*/� /*/� **7)� *)7;� -77*� ,;,.� )� /� +,--� +,7,� 7;*.79� .+;�

641 -)00� ,-77� +77+� *;+/� -00;� -07.� ;*/� )*))� *-;/� *07/� .).+� -.0*� /� 0;� -,,-� -,+/� 7;*.79� .+;�

645 +)//� )+;.� *,0,� )7,+� +/;7� )..*� /.)� ,+)� ).)0� ...� ,-*,� );*)� )0*� +)/� ,;00� )//;� 7;*.79��++*�

652 ,),;� +-.-� +**.� *,;7� ,)).� ,)+,� /.,� /7,� );.0� );,/� -,//� ,,0+� .+� 0;� -)+)� ,,;*� 7;*.79��)0.�

661 +./7� +./7� *,70� *,*)� +;,)� +00)� ;..� ;*/� )0+)� )/).� --*;� -7/;� 0*� /7� -7,/� ,-**� 7;*.79� .+;�

730 )*;,� ),0-� )7,)� )*//� ),.;� );..� *);� **/� -7+� .07� )//*� );/0� +,� ,-� ).,-� );/0� 7;*.79� )/;�

803 *.+-� *,,,� ***.� *7+7� ++.0� +7*.� 0;;� .07� )/,-� )-)*� +0*)� +;*7� +*,� */� ,.),� +.+;� 7;*.79>�7*;�

818 */;-� *-).� *-).� *,,)� ,)))� +.).� ;-;� 0;7� **,)� *)..� ,-+*� ,.))� *-� *-� +;7*� +/*7� 7;*.79>�7*;�

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2007 IBDV ring trial report page 12

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108 )*?)� )*?*� ))?.� ))?0� ))?;� )*?7� 0?;� ;?*� ))?*� ))?,� )*?*� )*?-� 0?7� /?.� )*?7� )*?*� 7;*.79� .+;�

158 0?,� ;?)� 0?,� 0?.� 0?-� ;?)� /?0� /?/� /?0� 0?)� 0?;� ;?+� .?7� .?)� 0?/� ;?-� 7;*.79� .+;�

207 ))?7� ))?,� )7?)� )7?;� ))?,� ))?+� ;?+� ;?+� )7?,� )7?+� ))?-� ))?-� /?7� 7?7� ))?-� ))?-� 7;*.79>�7*;�

217 ))?.� ))?.� ))?*� ))?+� ))?,� ))?;� ;?/� ;?/� )7?.� )7?,� )*?7� ))?0� -?;� .?+� )*?7� )*?7� 7;*.79� .+;�

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324 )*?7� ))?;� ))?-� ))?,� )*?*� )*?)� ;?;� ;?,� ))?7� )7?;� )*?,� )*?,� 7?7� 7?7� )*?,� )*?*� 7;*.79� .+;�

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641 )*?+� )*?)� ))?.� ))?-� )*?-� )*?-� ;?;� )7?*� ))?+� ))?-� )*?.� )*?-� *?0� .?-� )*?,� )*?,� 7;*.79� .+;�

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652 )*?7� ))?0� ))?/� ))?+� )*?7� )*?7� ;?.� ;?-� )7?;� )7?;� )*?,� )*?)� .?7� .?-� )*?+� )*?)� 7;*.79��)0.�

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730 )7?+� )7?-� )7?7� )7?+� )7?-� )7?;� /?0� /?0� ;?7� ;?,� )7?0� )7?;� -?)� -?-� )7?/� )7?;� 7;*.79� )/;�

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818 ))?,� ))?+� ))?+� ))?+� )*?7� ))?0� ;?;� ;?0� ))?)� ))?)� )*?)� )*?*� ,?/� ,?/� ))?;� ))?;� 7;*.79>�7*;�

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2007 IBDV ring trial report page 13

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217 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 9� 9� 8� 8� 7;*.79� .+;�

303 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 9� 9� 8� 8� 7;*.79"�7*;2� �+;�

324 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 9� 9� 8� 8� 7;*.79� .+;�

490 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 9� 9� 8� 8� 7;*.79"�7*;�

500 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 9� 9� 8� 8� 7;*.79� .+;�

515 8� 8� 8� 9� 8� 8� 8� 9� 8� 8� 8� 8� 9� 9� 8� 8� 7;*.79��)0.�

604 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 9� 9� 8� 8� 7;*.79� .+;�

641 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 9� 9� 8� 8� 7;*.79� .+;�

645 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 9� 9� 8� 8� 7;*.79��++*�

652 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 9� 9� 8� 8� 7;*.79��)0.�

661 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 9� 9� 8� 8� 7;*.79� .+;�

730 8� 8� 8� 8� 8� 8� 9� 9� 8� 8� 8� 8� 9� 9� 8� 8� 7;*.79� )/;�

803 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 9� 9� 8� 8� 7;*.79>�7*;�

818 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 8� 9� 9� 8� 8� 7;*.79>�7*;�

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2007 IBDV ring trial report page 14

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2007 IBDV ring trial report page 15

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labcode out mi zij1 zij2 sri mi-m

5 10,63 0,38 0,32 0,16 0,08

20 11,43 -0,23 - - 0,88

41 11,11 -0,61 - - 0,56

47 10,61 0,34 0,34 0,10 0,06

66 10,80 0,65 0,50 0,13 0,24

66 10,52 0,50 0,02 0,34 -0,04

108 11,06 0,72 0,89 0,16 0,50

158 � 8,18 -3,04 -2,52 0,18 -2,37

207 10,57 -0,10 -0,87 0,24 0,01

217 10,59 0,27 0,35 0,18 0,04

303 10,61 0,22 0,50 0,62 0,05

324 11,54 -0,01 -0,21 0,17 0,99

490 10,49 0,21 0,19 0,18 -0,06

500 10,58 0,48 0,17 0,27 0,03

515 10,23 0,04 -1,72 0,53 -0,32

604 10,79 -0,32 0,02 0,03 0,24

641 10,91 0,55 1,04 0,92 0,36

645 10,39 0,61 -0,68 0,83 -0,16

652 10,82 0,65 0,52 0,19 0,27

661 10,82 0,66 0,55 0,08 0,26

730 � 9,41 -1,43 -1,09 0,22 -1,14

803 10,59 0,63 -0,05 0,91 0,04

818 10,53 0,33 0,23 0,07 -0,02 � Out A Rwithin higher than 1.0 log2 (�) or trueness higher than 1.0 log2 (�) ;.� �!�������<������������-���

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108

158

207

217 303

324

490 500

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652 661

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2007 IBDV ring trial report page 16

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2007 IBDV ring trial report page 17

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Page 24: Report of the 2007 International Ring Trial for infectious ...alfabiolperu.com/pdf/pruebas_internacionales/2007... · Serology is widely used as a diagnostic tool and for predicting

2007 IBDV ring trial report page 24

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labcode sri ELISA brand / Remarks

335 0,07 Guildhay V090-03507

75 0,05 Guildhay V090-22407

76 0,41 Guildhay V090-45506

103 0,10 Guildhay V094-03507

47 0,07 Hipra CBD.4P2R

394 0,27 InHouse IBDV011204

545 - Synbiotics 96-6503 sample 7ND

730 0,04 Synbiotics 0602767

321 0,09 Synbiotics 107008

449 - Synbiotics IBD 0700087

449 - Synbiotics IBD+ 0602821

641 0,18 LDL 200IBD21

854 0,10 LDL 200IBD21

24 0,25 LDL 200IBD21 SRI Within laboratory reproducibility