Reproductive cloningBiotechnology/Cloning

Reproductive cloning

Therapeutic cloning

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Therapeutic cloning

Recombinant DNA technologyDNA isolated from

two sources and cut by the same enzyme

Gene of interest

RecombinantDNA

Plasmid(vector)

Transgenic organisms

Useful products

Gene for pestresistance

Gene fortoxic-cleanup

bacteria

Some uses of proteins:

Protein for“stone-washing”

jeans

Protein fordissolving

clots

Some uses of genes:

Restriction Enzymes

First Cloning ExperimentCohen et al., 1973“Recombinant DNA”

Cloning Vectors: pBR322 plasmid

Cloning into pBR322

RT-PCR and Cloning

Palmiter et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615

“Supermice”

Golden Rice

Weeding Out Land MinesFirst Glowing Pet