research article screening of pearl millet f hybrids for...
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Research ArticleScreening of Pearl Millet F1 Hybrids for Heat Tolerance atEarly Seedling Stage
Ashok Kumar Yadav12 Rajesh Kumar Arya2 and M S Narwal2
1 CSIR-Institute of Himalayan Bioresource Technology Palampur Himachal Pradesh 176061 India2Department of Genetics and Plant Breeding CCS HAU Hisar 125004 India
Correspondence should be addressed to Ashok Kumar Yadav ashokihbtresin
Received 30 April 2014 Revised 7 August 2014 Accepted 26 August 2014 Published 2 October 2014
Academic Editor Clifford Gold
Copyright copy 2014 Ashok Kumar Yadav et al This is an open access article distributed under the Creative Commons AttributionLicense which permits unrestricted use distribution and reproduction in any medium provided the original work is properlycited
Ten pearl millet genotypes selected on the basis of response to supra-optimal temperature tolerance were crossed in a half-diallelmating system The 45 F
1hybrids produced were tested along with parents for heat tolerance and related traits at seedling stage
Field screening and laboratory screening techniques were simultaneously used for the evaluation of F1hybrids and their parents
Heat tolerance was measured as seedling thermotolerance index (STI) and seed to seedling thermotolerance index (SSTI) underfield conditions but membrane thermostability (MTS) in the laboratory The hybrid H7729-2 times CVJ-2-5-3-1-3 showed highestSTI value followed by H77833-2 times 96AC-93 The genotype H77833-2 times 96AC-93 had the highest worth for SSTI These threeindices were highly correlated among themselves STI values were invariably high whereas SSTI has lower values as it also coversthe effect of under soil mortality (USM) It was seen that the heat tolerance indices STI and SSTI were not showing any perceptiblepooled correlation with developmental traits except germination and emergence rate Based on our results it could be suggestedthat membrane thermostability (MTS) may be used for screening large number of genotypes Field based indices STI and SSTI maybe used for evaluation of hybrids and varieties before they are released
1 Introduction
Pearl millet [Pennisetum glaucum (L) R Br] is a multipur-pose cereal grown for grain stover and green fodder It is themost important staple crop in the semiarid and arid regionsof Asia and sub-Saharan Africa It shall continue to play aprominent role in the integrated agricultural and livestockeconomy of the country particularly in rainfed areas dueto its drought hardiness Pearl millet provides an excellentnutritious food because of high biological valuemore protein(116) fat (5) and mineral content (23) It also containsadequate amount of essential amino acids such as tryptophanthreonine arginine and lysine Pearl millet is also high inlipids and calcium and low in crude fibre
Adequate crop stand establishment problems are severeconstraints to get good production of pearl millet Failure toobtain adequate plant populations is often associated with
high temperatures occurring during the germination andseedling establishment period of pearl millet The temper-ature is one of the key climatic factors and has profoundeffect on the growth and development of the pearl milletThe soil temperatures in farmersrsquo field in India and Africacommonly exceed 45∘C and the temperatures as high as 60∘Chave occasionally been measured [1ndash3] The supraoptimaltemperatures are known to be the most important cause ofpoor crop stands in farmerrsquos field This can only be managedthrough developing hybrid varieties which can tolerate hightemperature during germination and early seedling stagesGenetic variability for adaptation to high temperature existsin crop plants Screening of F
1crosses and identification of
the superior cross combinations for seedling heat tolerance isessential for effective manipulation through hybrid breedingThe objective of this study was to understand the responseof different cross combinations for heat tolerance during
Hindawi Publishing CorporationAdvances in AgricultureVolume 2014 Article ID 231301 17 pageshttpdxdoiorg1011552014231301
2 Advances in Agriculture
Table 1 Description of pearl millet genotypes used as parents for hybridization
Sr No Genotype Response Status Origin and characteristics
1 H77833-2 Tolerant Inbred Bred at CCSHAU Hisar by selfing and selection within a Rajasthanilandrace population
2 H7729-2 Susceptible InbredBred at CCSHAU Hisar It is medium dwarf nodalpigmentationmdashviolet brown resistant to downy mildew shoot flygrey weevil chaffer beetle and leaf roller
3 G73-107 Tolerant Inbred Bred at CCSHAU Hisar and has profuse tillering narrow leaves andtypical orange brown anthers
4 77245 Susceptible Inbred Bred at CCSHAU Hisar by selfing and selection It is late floweringand high tillering
5 CVJ-2-5-3-1-3 Tolerant Inbred Dwarf developed at ICRISAT Patancheru
6 1305 Susceptible Inbred Bred at CCSHAU Hisar late flowering medium dwarf semicompactearhead
7 (77371 times BSECT CP-1) Tolerant Inbred Bred at CCSHAU Hisar using BSECT CP-1 from ICRISATPatancheru as male parent
8 96AC-93 Tolerant Inbred Resistant to downy mildew bred at CCSHAU Hisar by selfing andselection
9 Togo-II Tolerant Inbred Selection from ICRISAT material-Togo bred at CCSHAU Hisar10 99HS-18 Tolerant Inbred Bred at CCS Haryana Agricultural University Hisar
Table 2 Details of different environments created through sowing dates
Environment Nature of environment Date of sowing Site Location
Environment-1 (E1) Heat stress May 21 Research area Department ofGenetics and Plant Breeding
CCS HAU Hisar Haryana
Environment-2 (E2) Heat stress June 13 Research area Department ofGenetics and Plant Breeding
CCS HAU Hisar Haryana
Environment-3 (E3)(during monsoon season)
Nonstress July 17 Research area Department ofGenetics and Plant Breeding
CCS HAU Hisar Haryana
germination and early seedling stages in pearl millet and toidentify some promising F
1hybrids improvement for this
trait particularly for commercial cultivation
2 Materials and Methods
21 Plant Material A field screening technique that isthermotolerance index [4] was applied to screen the pearlmillet F
1hybrids for heat tolerance at seedling stage This
technique could be used in the hottest months (May-June)only with no rainfall Since the technique was applicableto the very young seedlings of pearl millet it was also ofconcern to test the association of seedling thermotoleranceto other phenotypic characteristics of seedlings The mem-brane thermostability (based on quantification of electrolyteleakage) was also conducted for screening the pearl milletF1hybrids at seedling stage Ten pearl millet genotypes
namely H77833-2 H7729-2 G73-107 77245 CVJ-2-5-3-1-3 1305 77371 times BSECT CP-1 96AC-93 Togo-II and 99HS-18 were selected from the germplasm on the basis of differentresponse to supraoptimal temperature tolerance (Table 1) inseparate experiment [5] These were crossed in a half-diallelmating system (excluding reciprocals) The 45 F
1hybrids
produced were tested along with parents for heat toleranceand related traits at seedling stage
22 Experimental Site and Conditions of Growth The exper-iment was conducted at the research area of Departmentof Genetics and Plant Breeding Chaudhary Charan SinghHaryana Agricultural University Hisar (Lat 29∘ 101015840N Long75∘ 461015840E and 2152m above mean sea level) located insubtropical region of Haryana India The 45 F
1hybrids
produced were tested along with parents for heat toleranceand related traits in three replicated plots under supraop-timal temperature exposure at seedling stage in two stressenvironments (environment-1 and environment-2) createdthrough different dates of sowing on May 21 and June 13 (thehottest period in North India) respectively so as to conductthe experiment under different range of temperatures withno rainfall A presowing medium light irrigation was donethrough flooding and the field was precisely levelled beforesowing After sowing no further irrigation was given to thestressed environments (environment-1 and environment-2)One nonstress environment (Table 2) was created throughsowing on July 17 (normal sowing period during monsoon)Each genotype was grown in 3 rows each of 3m length spaced
Advances in Agriculture 3
0
10
20
30
40
50
60
70
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15DAS
E1
E2
E3
Tem
pera
ture
(∘C)
(a)
05
101520253035404550
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15DAS
E1
E2
E3
Tem
pera
ture
(∘C)
(b)
0
10
20
30
40
50
60
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16DAS
E1
E2
E3
Tem
pera
ture
(∘C)
(c)
02468
101214161820
1 3 5 7 9 11 13 15
Moi
sture
()
DAS
E1
E2
E3
Permanent wilting point
(d)
Figure 1 (a) Soil surface temperature (b) air temperature (c) temperature 5 cm above the soil surface and (d) soil moisture status in differentenvironments
30 cm apart with 10 cm plant to plant spacingThe seeds weresown manually
The soil of experimental field was sandy loam in textureThe water holding capacity field capacity and permanentwilting point of the experimental field were 40 17 and 6percent respectively [6] Irrigation was withheld during thecourse of experiment however drought stress-free condi-tions were ensured through monitoring at regular interval(on alternate days) by measuring the moisture status of thesoil by gravimetric method The soil samples of about 50 gwere taken up to 20 cmdepth from the experimental plotThemoisture content on dry weight basis may be calculated [7]using the following formula
Soil moisture ()
=
(Weight of wet soil minusWeight of dry soil)Weight of dry soil
times 100
(1)
The heat stress period was terminated by irrigating theexperimental field before reaching the soil moisture level atpermanent wilting point A condition of no drought wasmaintained in order to determine the exclusive effect of tem-perature at seedling stageThis was maintained by measuringthe moisture status of the soil by gravimetric method onalternate days The soil surface temperature was measuredby a soil thermometer at soil surface temperature at 5 cmabove the soil surface and air temperature at 1m above thesoil surface were recorded between 200 pm and 230 pmdaily (Figure 1)
Emphasis of observation is centred on germination sur-vival and mortality of the seedlings
23 Plant CharacteristicsScreening Techniques The empha-sis was given to the observations of germination survival andmortality of the seedlingsThe seedlings were inspected everyday in the morning The seedlings which had died due to
4 Advances in Agriculture
heat stress were noted in each genotype on daily basis Obser-vations continued till the surviving seedlingswere established(15 DAE) and there was no further mortality Moreover thesoil moisture was depleting near to the permanent wiltingpoint and drought stress could also come into play along withheat stress to further continue the experiment Since experi-ment proceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedlingsurvival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of heat stress at seedling stage was observed on latergrowth stages
231 Thermotolerance Characteristics For heat tolerancecharacteristics 3 indices namely seedling thermotoleranceindex (STI) [4] seed to seedling thermotolerance index(SSTI) [8] and membrane thermostability (MTS) wereapplied to evaluate the hybrids and parents STI was cal-culated as the ratio of number of seedlings survived to thenumber of seedling emergences expressed in percentageSeed to seedling thermotolerance index (SSTI) was calculatedas the ratio of seedling survival to the number of seedlingsexpected to emerge expressed in percentage [8] The SSTIis an extension of STI by taking expected germination intoaccount It was necessary to correct the effect of under soilmortality (USM) The loss of germination due to heat (ieunder soil mortality) can be measured only after a standardgermination test Therefore the germination of seed fromthe same lot under monsoon environment (main pearl milletgrowing season where no under soil mortality occurreddue to heat stress) was taken as expected germination forcalculation of SSTI
232 Membrane Thermostability These genotypes were alsoscreened through laboratory technique (membrane ther-mostability) [9 10] The young leaves of 21-day-old seedlingsof each genotype in three replication plots were used formembrane thermostability test A middle portion of 10 cmfrom each leaf was taken and the midrib was removed andtwo equal halves of the 10 cm each were made Each halfwas taken to provide replications Each leaf portion was cutinto pieces and thoroughly washed and rinsed with distilledwater two times to remove the electrolytes adhering to theplant tissue as well as electrolyte released from the cutting ofplant tissue and put them into test tubes After final rinsingtubes were drained maintaining sufficient water to preventdesiccation of plant material during heat treatment Thesetest tubes were covered with aluminium foil and incubated inwater bath at 55∘C for 15 minutes for giving heat treatmentAfter the heat treatment period 10mL of distilled waterwas added to the test tubes Then all the test tubes wereheld at 10∘C overnight to allow diffusion of electrolytes fromplant material Test tubes were brought out of refrigeratorand shaken well to mix the contents An initial electricalconductivity (EC) of both replications of each treatment (119879
1)
was determined with electrical conductivity meter Thenthese test tubes were autoclaved at 15 psi for 2 minutes forcomplete electrolyte leakage from the leaf samples These testtubes were then cooled at room temperature and contentsmixed and final EC was taken for all the treatments (119879
2)
Membrane thermostability (MTS) was calculated accordingto Ibrahim and Quick [9] and Yadav et al [10] as follows
Membrane Thermostability (MTS) = (1 minus1198791
1198792
) times 100
(2)
where 1198791is conductivity reading after heat treatment and 119879
2
is conductivity reading after autoclavingCompletely randomized design (CRD) for membrane
thermostabilitywas conducted to detect variations among thegenotypes
233 Morphological Characteristics The seedling growthcharacteristics namely germination () emergence rate(ER) number of leavesseedling (two-week stage) seedlingheight (two-week stage) seedling fresh weight (g) (four-week stage) and seedling dry weight (g) (four-week stage)were recorded The data for seedling height and number ofleaves per seedling was recorded on 10 random seedlings perplot Emergence rate (an index for speed of germination)can be used as a tool in pearl millet breeding programs forevaluation of seedling vigour Speed of germination is oneof the oldest concepts of seedling vigour The emergencerate (ER) was calculated by dividing the number of normalseedlings obtained at each counting by the number of daysseeds had been in the seed bed (DAS)The values obtained ateach count were then summed at the end of the germinationtest to obtain the emergence rate [11]
Emergence Rate (ER) =Seedling count
Days to first count+ sdot sdot sdot
+
Seedling countDays to final count
(3)
After termination of the heat stress on the seedlings byirrigating the field the experiment was continued to studythe effect of seedling heat stress on maturity traits on thesurvived plants The maturity traits observed were panicleemergence effective tillersplant plant height ear length earweightplant dry fodder yieldplant grain yieldplant andtotal biological yieldplant
24 Statistical Analysis Analysis of variance for randomizedblock design (RBD) for field traits that is germinationemergence rate number of leavesseedling seedling heightseedling fresh weight and seedling dry weight and com-pletely randomized design (CRD) for membrane thermosta-bility test was conducted under each environment on meanbasis [12] Analyses were done using the software OPSTATdeveloped at CCS HAU Hisar Correlations among the vari-ous characters studied were calculated to establish the natureand magnitude of associations among the traits [8 13] with
Advances in Agriculture 5
the software SPSS (SPSS Inc) Multivariate clustering wasdone based on paired group cluster method using similaritycoefficient under Past 140 software [14] Multivariate analysisbased on the Euclidean distancematrix was estimated amonggenotypes to distinguish genotypes from each other Thegrouping of genotypes was generated using paired groupcluster method
3 Results and Discussion
The excessive heat (soil temperature up to 630∘C in E2)
about 18∘C higher than the normal atmospheric temperature(Figures 1(a) and 1(b)) was responsible for mortality ofseedlings Many seedlings were found desiccated at the pointof contact besides the desiccation at the tipsThe temperaturearound the tip level (5 cm above the soil surface) also reachedup to 50∘C (Figure 1(c)) It could therefore be concluded thatthe seedlings had to survive in a really high temperature Themoisture level remained above the permanent wilting point(Figure 1(d)) therefore drought effect was not in the playThe heat tolerance was measured as STI (Table 3) throughthe field screening technique of Peacock et al [4] Anotherindex known as SSTI was also calculated as an improvementand extensionThe analysis of variance (RBD) for 8 charactersin 3 environments showed significant genotypic differencesNo variability was observed for STI and SSTI in E
3only In
this environment no seedling mortality was observed andthe germination was taken as standard The values for STIand SSTI were therefore equal and the highest for all thegenotypes in this environment and hence no variation wasobserved
The hybrids were evaluated on the basis of mean perfor-mance for various characters in each environment (Table 3)On the mean basis STI value ranged from 5332 to 8224with an average of 7072 The hybrid H7729-2 times CVJ-2-5-3-1-3 was the best scorer for heat tolerance followed byH77833-2 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II The hybrid H77833-2 times 1305 was found susceptible toheat stress Seed to seedling thermotolerance index (SSTI)which is expected to be more rigorous index deviated thevalues from STI according to variation in germination undernormal environment (E
3) as the SSTI also coversincludes
the under surface mortality (USM) during germinationThe reductions in values were not strictly proportional tothe STI The reduction is dependent on thermosensitivityof germination We also came across such hybrids whichshowed poor germination and high STI SSTI values rangedfrom 4605 to 7287 with an average of 6090 The genotypeH77833-2 times 96AC-93 had the highest worth for SSTI onmean basis followed byG73-107times (77371timesBSECTCP-1) andH77833-2 times (77371 times BSECT CP-1) (Table 3) The analysisof variance for ten different characters in three differentenvironments (two stressed and one normal) was carried outand the results showed significant genotypic differences forall the characters (Table 4)
A caution is needed for evaluating such genotypes underSTI The SSTI gave a highly reduced value putting thesecrosses in the susceptible list It may be concluded therefore
that SSTI is more dependable and desirable tool for screeningand evaluating the genotypes for heat tolerance The germi-nation was lower in E
2than in E
1 This period has the highest
range of temperature with a high average Genotype-wise thehighest germination (6209) was recorded for the genotypeH7729-2 times (77371 times BSECT CP-1) on mean basis whichgives a preliminary indication of resistance to heat while thegenotype 1times 6 had the lowest germination (3533)The hybridH77833-2 times G73-107 was adjudged as the fastest emergenceof seedlingsThe genotype (77371timesBSECTCP-1)times 96AC-93had the highest fresh weightseedlingThe genotype H7729-2 times Togo-II had the highest seedling dry weight E
1had lower
dry weightseedling than E2indicating the more heat stress
during the E1
31 Membrane Thermostability Membrane thermostability(MTS) has long been recognised as a parameter of heattolerance All the hybrids were put to membrane thermosta-bility test in the laboratory under standard protocol Acompletely randomised design (CRD) analysis was carriedout for membrane thermostability (MTS) The mean sum ofsquares due to genotypes was highly significant indicatingthat enough genetic variability was present for membranethermostability (MTS) in each environment which justifiedfurther analysis of the data The range of values lay between5073 and 7742 Among the parents H77833-2 G73-107CVJ-2-5-3-1-3 77371 times BSECT CP-1 96AC-93 Togo-II and99HS-18 were verified as resistant genotypes and H7729-2 77245 and 1305 genotypes were found susceptible asmentioned in Table 1 (on the basis of previous experiment)Among all the hybrids H7729-2 times CVJ-2-5-3-1-3 CVJ-2-5-3-1-3 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II werethree highly tolerant genotypes on the basis of membranethermostability (Table 5)
32 Correlations The pooled correlations among the charac-ters were calculated (Table 6) around heat tolerance indices(STI SSTI and membrane thermostability) with a view tofind whether there was any association of these characterswith seedling as well as maturity traits It was observedthat STI and SSTI recorded the highest significant cor-relation among themselves as they are related indices forheat tolerance Membrane thermostability also showed highsignificant correlation with STI as well as SSTI STI hada significant positive association with germination of seedonly among seedling and maturity traits studied SSTI inaddition was also positively correlated with germination andemergence rate and negatively associated with number ofeffective tillersplantMembrane thermostability also showedsignificant positive correlation with seed germination amongseedling and maturity traits studied While evaluating thediallel progenies it was also seen that the heat toleranceindices STI SSTI and membrane thermostability were notshowing any perceptible correlation with the rest of thedevelopmental traits (seedling andmaturity traits)The stressperiod was terminated by irrigating the field experiment after15 days fromdate of sowingwhen the seedlings get establishedand further mortality stopped due to heat Moreover the soil
6 Advances in Agriculture
Table3Perfo
rmance
ofthep
arentsandthec
rosses
ford
ifferentcharacters
(a)
Sr
No
Genotypes
Germination(
)Ra
teof
emergencelowast
STIlowast
()
SSTIlowast(
)Num
bero
fleavesseedlin
gE 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
3941
3737
6164
4614
4037
4109
4279
4142
7830
7387
9000
8072
4498
4133
9000
5877
661
502
800
654
2H7729-2
5513
4726
6092
5443
2815
4980
3258
3684
4835
4495
9000
6110
4441
3602
9000
5681
363
294
427
361
3G73-107
5037
4826
5918
5260
4534
4132
4481
4382
6187
5413
9000
6866
5213
4460
9000
6224
878
710
935
841
477245
4747
4624
5565
4978
3625
4604
4047
4092
4385
3580
9000
5655
3803
3040
9000
5281
377
292
437
369
5CV
J-2-5-3-1-3
4824
4832
6897
5518
5044
4907
4960
4970
7193
6813
9000
7669
4959
4785
9000
6248
802
580
923
768
61305
4326
3891
5404
4540
3376
5056
3573
4002
3903
3281
9000
5395
3211
2452
9000
4888
350
284
414
349
777371timesBS
ECTCP
-14747
4502
6320
5190
4934
5075
5065
5025
6952
6706
9000
7552
5056
4671
9000
6242
621
542
700
621
896AC
-93
4651
3818
5722
4730
4312
4915
4753
4660
6660
6001
9000
7220
5212
3920
9000
6044
701
590
899
730
9To
go-II
4613
3937
5276
4609
4245
4737
4797
4593
7198
6861
9000
7686
5980
4767
9000
6582
681
629
853
721
1099HS-18
4575
3823
6247
4882
4317
4659
4746
4574
7594
7319
9000
7971
5154
4172
9000
6109
745
554
915
738
1H77833-2timesH7729-2
5787
5295
6733
5938
4964
5015
4989
4989
6803
6504
9000
7436
5808
5134
9000
6647
711
585
780
692
2H77833-2timesG73-107
5472
4417
6405
5431
5448
5422
5239
5370
7231
6769
9000
7667
5980
4556
9000
6512
1101
705
1340
1049
3H77833-2times77245
4076
3707
5504
4429
5217
5446
5238
5300
6627
6224
9000
7284
4690
4037
9000
5909
740
525
965
744
4H77833-2timesCV
J-2-5-3-1-3
4843
4300
5856
5000
4862
4956
4913
4911
7061
6519
9000
7527
5575
4632
9000
6403
998
885
1258
1047
5H77833-2times1305
3296
2780
4521
3533
5076
4907
5238
5074
3732
3265
9000
5332
2765
2050
9000
4605
1192
934
1600
1242
6H77833-2times(77371times
BSEC
TCP
-1)5636
5539
6258
5811
4489
5219
5015
4908
6981
6482
9000
7488
6146
5680
9000
6942
898
726
1525
1050
7H77833-2times96AC
-93
5720
5515
6335
5857
4800
5186
5075
5021
7833
7786
9000
8206
6603
6256
9000
7287
1107
873
1193
1058
8H77833-2timesTo
go-II
4862
3453
5709
4675
5036
4829
4911
4925
6190
5500
9000
6897
5214
3339
9000
5851
979
670
1107
919
9H77833-2times99HS-18
4575
4418
5447
4813
5041
5074
5006
5040
5800
5073
9000
6625
4846
4133
9000
5993
978
733
1135
949
10H7729-2timesG73-107
5615
4962
6273
5617
5023
5535
5165
5241
6143
5501
9000
6881
5540
4441
9000
6327
1000
720
1363
1028
11H7729-2times77245
5472
5164
6281
5639
4745
5031
5028
4935
5572
5175
9000
6582
4901
4345
9000
6082
880
620
897
799
12H7729-2timesCV
J-2-5-3-1-3
5494
5124
6536
5718
4804
5316
4957
5026
7596
8076
9000
8224
6093
5680
9000
6924
981
932
1451
1121
13H7729-2times1305
3804
3826
5511
4380
3596
4724
4387
4236
6362
7075
9000
7479
4230
4517
9000
5916
926
725
1497
1049
14H7729-2times(77371times
BSEC
TCP
-1)
6369
4847
7412
6209
4847
5351
5246
5148
6377
6031
9000
7136
5683
4230
9000
6304
977
671
1380
1009
15H7729-2times96AC
-93
5314
4657
6702
5558
4514
5295
5102
4971
5612
4856
9000
6489
4613
3603
9000
5739
846
679
1397
974
16H7729-2timesTo
go-II
5827
5594
6651
6024
5072
5279
5271
5207
6563
5371
9000
6978
5763
4652
9000
6472
873
719
1040
877
17H7729-2times99HS-18
5741
4638
7918
6099
4847
5024
5021
4964
6179
5603
9000
6927
4882
3725
9000
5869
1012
730
1600
1114
18G73-107times77245
5115
4646
6103
5288
4977
5242
5450
5223
7006
6063
9000
7356
5685
4594
9000
6426
1021
790
1292
1034
19G73-107timesCV
J-2-5-3-1-3
5134
4894
6427
5485
4901
5317
5181
5133
6637
6124
9000
7254
5253
4710
9000
6321
1039
863
1254
1052
20G73-107times1305
4881
4561
7597
5680
4717
4791
4775
4761
6704
6380
9000
7361
4556
4115
9000
5890
1140
882
1657
1226
21G73-107times(77371times
BSEC
TCP
-1)
5636
5519
6283
5812
4986
5219
5203
5136
7990
6203
9000
7731
6620
5436
9000
7019
977
703
1395
1025
22G73-107times96AC
-93
5740
4510
6415
5555
5129
4982
5195
5102
6978
5556
9000
7178
6156
4019
9000
6392
1077
860
1264
1067
23G73-107timesTo
go-II
4460
4357
6044
4953
4396
5138
4951
4829
5279
4835
9000
6371
4018
3603
9000
5540
906
881
1189
992
24G73-107times99HS-18
5473
4356
6374
5401
5102
4955
5055
5037
6460
5197
9000
6886
5534
3704
9000
6079
1171
766
1635
1191
2577245timesCV
J-2-5-3-1-3
4766
4666
5921
5117
5170
4918
4900
4996
5928
5859
9000
6929
4785
4613
9000
6133
984
920
1194
1033
2677245times1305
4575
4060
6052
4895
5128
5297
5203
5210
5102
4990
9000
6364
3979
3464
9000
5481
1279
823
1550
1217
2777245times(77371times
BSEC
TCP
-1)
5017
4929
6022
5322
5108
5473
5274
5285
5308
5044
9000
6451
4498
4191
9000
5896
833
588
939
787
Advances in Agriculture 7
(a)Con
tinued
Sr
No
Genotypes
Germination(
)Ra
teof
emergencelowast
STIlowast
()
SSTIlowast(
)Num
bero
fleavesseedlin
gE 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
2877245times96AC
-93
5472
4705
622154
66
5135
5197
5006
5113
5247
4843
9000
6363
4708
3784
9000
5831
795
662
1058
838
2977245timesTo
go-II
5252
4492
5889
5211
5188
4861
5074
5041
4885
4263
9000
6049
4421
3379
9000
5600
977
751
1243
990
3077245times99HS-18
5740
4099
6872
5570
5249
4898
5258
5135
5628
4616
9000
6415
4863
3014
9000
5626
1045
863
1184
1031
31CV
J-2-5-3-1-3times1305
4364
3944
5700
4669
4974
4517
4650
4714
6097
5579
9000
6892
4594
3842
9000
5812
936
848
1403
1063
32CV
J-2-5-3-1-3times(77371times
BSEC
TCP
-1)4345
3808
6774
4976
5272
4996
5035
5101
6650
5766
9000
7139
4288
3381
9000
5556
800
712
1013
841
33CV
J-2-5-3-1-3times96AC
-93
4862
4197
5743
4934
4884
4980
5058
4974
7477
6760
9000
7745
5957
4709
9000
6555
933
738
1478
1050
34CV
J-2-5-3-1-3timesTo
go-II
4670
4343
6233
5082
4399
4813
4631
4614
5806
6614
9000
7140
4422
4479
9000
5967
963
855
1180
999
35CV
J-2-5-3-1-3times99HS-18
5493
5080
7273
5949
5276
4984
5162
5141
7322
6590
9000
7637
5534
4766
9000
6433
998
807
1338
1047
361305times(77371times
BSEC
TCP
-1)
4613
4539
5717
4956
4986
4955
5080
5007
5137
4870
9000
6336
4210
3936
9000
5715
863
630
913
802
371305times96AC
-93
5473
5177
6830
5827
5224
4779
4919
4974
7117
6965
9000
7694
5640
5220
9000
6620
875
694
1476
1015
381305timesTo
go-II
4230
3420
5262
4304
4309
456144
45
4438
5781
4373
9000
6385
4575
2887
9000
5487
637
566
794
666
391305times99HS-18
5433
4507
6947
5629
5225
4753
4991
4989
7287
6968
9000
7752
5576
4479
9000
6352
1115
818
1410
1114
40(77371times
BSEC
TCP
-1)times
96AC
-93
5595
4429
7461
5828
5216
4955
5121
5097
7087
6368
9000
7485
5432
4038
9000
6157
955
753
1462
1057
41(77371times
BSEC
TCP
-1)times
Togo-II
5252
4728
6106
5362
5492
4808
5132
5144
8025
7505
9000
8177
6310
5413
9000
6908
859
693
990
848
42(77371times
BSEC
TCP
-1)times
99HS-18
5595
4298
6295
5396
4729
4887
5031
4883
7366
6945
9000
7770
6312
4556
9000
6623
903
622
1125
883
4396AC
-93timesTo
go-II
5017
3977
6486
5160
5019
5195
5237
5150
5922
5486
9000
6803
4689
3484
9000
5724
1007
728
1167
967
4496AC
-93times99HS-18
5595
4812
6924
5777
5036
5385
5205
5209
7411
5378
9000
7263
5873
3979
9000
6284
1013
652
1334
1000
45To
go-IItimes
99HS-18
4670
4497
7293
5487
4718
4319
4796
4611
5688
5431
9000
6706
3980
3665
9000
5548
939
653
1462
1018
Mean
5043
4482
6271
5265
4792
498149
23
4899
6377
584190
00
7072
5079
4190
9000
6090
898
701
1168
922
SE097
106
161
mdash213
191
190
mdash19
818
7mdash
mdash14
914
6mdash
mdash036
047
068
mdashCD
272
296
453
mdash597
537
534
mdash555
525
mdashmdash
421
409
mdashmdash
103
133
192
mdashlowastTransfo
rmed
data
(b)
Sr
No
Genotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
908
785
2245
1313
859
1537
1924
1440
154
143
160
152
6267
4800
3833
4967
212
227
249
229
15644
15458
13267
1479
02
H7729-2
1125
1175
1789
1363
317
2400
2811
1843
093
191
252
179
6700
5333
5100
5711
185
210
234
210
18850
18565
15389
17601
3G73-107
1166
1475
1966
1536
943
1744
4945
2544
143
129
366
213
6700
5433
5300
5811
218
219
238
225
15009
1496
513503
14492
477245
1438
1373
2085
1632
607
1863
5957
2809
117
180
509
269
6100
4800
5100
5333
232
221
253
235
14036
13802
11742
1319
35
CVJ-2-5-3-1-3
1214
1437
1807
1486
1163
1610
4166
2313
171
141
407
240
6300
4933
4600
5278
239
227
270
245
1994
317261
1497
017391
61305
858
1137
1737
1244
540
967
4861
2123
125
088
417
210
6533
5033
4800
5456
244
213
257
238
15527
15405
12274
14402
8 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
777371timesBS
ECT
CP-1
1377
1487
1892
1585
928
2660
3330
2306
150
258
301
236
5733
4533
4700
4989
242
220
293
252
1513
814550
1315
014279
896AC
-93
1303
1259
1852
1471
1056
1445
5972
2825
185
147
586
306
5500
4833
3833
4722
256
304
318
293
15577
14032
10000
13203
9To
go-II
1056
1220
2272
1516
924
1466
9113
3834
146
129
883
386
6167
4533
4600
5100
185
149
124
153
14578
12073
10033
12228
1099HS-18
1277
1075
1808
1387
1241
2833
7070
3715
228
226
615
356
6100
5000
4167
5089
243
173
324
247
12627
10355
1017
311052
1H77833-2times
H7729-2
1432
1498
2233
1721
1350
4405
8373
4709
159
499
782
480
5767
4433
3800
4667309
290
248
282
21010
18071
15847
18309
2H77833-2times
G73-107
1675
1635
2205
1838
1236
3211
5789
3412
134
313
547
331
5833
4767
3833
4811
262
295
236
265
21253
20449
15808
1917
0
3H77833-2times
77245
1577
1522
2263
1787
1058
6933
8719
5570
255
553
782
530
5267
4633
4600
4833
273
201
265
246
2074
119511
15208
18487
4H77833-2times
CVJ-2-5-3-1-3
1791
1757
2165
1904
20218030
5276
5109
354
779
477
537
5533
4233
3767
4511
165
330
351
282
21827
20072
17110
19670
5H77833-2times1305
1700
1842
28412128
1427
6228
9134
5596
570
579
813
654
5800
4267
3333
4467340
426
451
406
19816
19444
16037
18432
6H77833-2times
(77371times
BSEC
TCP
-1)
1453
1515
3120
2029
789
465510428
5291
211
408
858
492
5233
4167
3267
4222
203
283
267
251
20677
1913
816470
1876
2
7H77833-2times
96AC
-93
1835
1783
2427
2015
2532
6649
1610
684
29
350
650
1473
824
5467
4233
3700
4467243
239
256
246
22278
20434
15735
19482
8H77833-2times
Togo-II
1564
15632500
1876
1882
4772
11660
6105
296
469
972
579
5667
4533
440048
67
169
260
211
213
2412
82375
619389
22424
9H77833-2times
99HS-18
159318002049
1814
1571
480214024
6799
203
544
1547
765
6033
460040
0048
78
261
225
256
247
23466
21081
1793
320
827
10H7729-2times
G73-107
14951568
2075
1713
959
5157
8517
4877
218
382
666
422
6133
5067
4833
5344
300
273
239
271
23651
2191
717083
20884
11H7729-2times77245
1557
1537
2613
1902
1851
5756
5729
4445366
562
524
484
5633
4800
4633
5022
133
233
297
221
1979
219914
15250
18319
12H7729-2times
CVJ-2-5-3-1-3
18442033
28932257
1651
806114885
8199
264
597
1054
638
6333
4600
4233
5056
170
211
217
199
24629
23339
1970
022556
13H7729-2times1305
1325
1677309920
34
1756
724314048
7682
318
635
1232
728
630046
67
3633
4867298
261
361
307
21666
19371
17422
19486
14H7729-2times(77371
timesBS
ECTCP
-1)
1958
1887
3105
2317
1118
4675
14411
6734
242
457
1318
672
5900
4500
3800
4733
164
209
240
204
23661
1995
419250
20955
15H7729-2times
96AC
-93
1512
1717
2779
2002266844
08
11393
6156
356
500
1211
689
5633
4533
4200
4789
221
195
282
232
2194
921635
17213
20266
Advances in Agriculture 9
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
16H7729-2times
Togo-II
1519
1738
2159
1805
2437
9867
1616
294
89
361
961
1527
950
6167
4700
4200
5022
234
272
230
246
26338
2414
520553
23678
17H7729-2times
99HS-18
1938
1787
3151
2292
1574
6218
11318
6370
290
606
956
617
6067
4767
406749
67
172
210
199
194
24689
21854
1778
921444
18G73-107times77245
1856
1813
2210
1960
2435
8550
7828
6271338
633
612
528
5500
4633
440048
44
199
255
307
254
1993
619277
16095
18436
19G73-107times
CVJ-2-5-3-1-3
1517
1769
2407
1897
1556
7728
9136
6140
239
876
917
677
5900
4300
3400
4533
233
266
315
272
23418
22333
19317
21689
20G73-107times1305
1217
1507
2832
1852
1777
5189
1114
960
38
289
588
1197
691
6733
5000
4233
5322
286
210
312
269
2078
81992
817067
19261
21G73-107times(77371
timesBS
ECTCP
-1)
18551946
2730
2177
1801
3482
10415
5233
239
277
753
423
5500
4467
3433
4467243
197
273
238
23817
20263
17425
20502
22G73-107times
96AC
-93
1718
1840
28612140
1345
9333
14669
8449
297
845
1278
807
5600
5033
4633
5089
270
243
300
271
28537
21461
16875
22291
23G73-107timesTo
go-II
168018083087
2192
1112
4678
9319
5036
186
473
985
548
6033
4767
4200
5000
210
209
231
217
24864
24609
21625
23699
24G73-107times99HS-18
1398
1675
3370
2148
2753
8672
11918
7781
362
691
981
678
566746
00
3500
4589
170
247
270
229
24677
20567
17855
21033
2577245times
CVJ-2-5-3-1-3
1500
1728
2203
1810
2282
4679
11492
6151
328
424
1116
623
6000
4333
420048
44300
231
370
300
21257
20497
1772
519826
2677245times1305
143815772080
1698
949
10439
12053
7814
121
915
992
676
5767
4800
3500
4689
168246
316
244
1978
91779
015517
17699
2777245times(77371times
BSEC
TCP
-1)
150516972846
2016
3222
8518
12621
8120
469
827
1141
812
5400
4367
3833
4533
196
225
242
221
2092
819485
1719
219202
2877245times96AC
-93
1653
1797
2117
1856
1934
6056
11543
6511
352
563
911
608
5333
4267
3833
4478
186
169
198
184
22003
19054
1676
719275
2977245timesTo
go-II
1723
1718
3163
2201
1749
8250
15443
8481229
806
1412
816
5967
4700
4067
4911
174
245
298
239
22075
23503
19875
21818
3077245times99HS-18
2016
1970
28542280
3217
13027
3531
6591395
965
285
549
5733
4467
3833
4678
148
228
209
195
2212
721462
1690
020
163
31CV
J-2-5-3-1-3times
1305
1358
1422
2179
1653
1254
2803
1019
44750
178
254
801
411
6033
4467
3367
4622
312
193
330
278
22269
19547
17250
19689
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
1335
1742
2400
1826
922
5226
12806
6318
236
458
1054
583
5633
4433
3600
4556
321
315
259
298
2210
419524
17087
19572
33CV
J-2-5-3-1-3times
96AC
-93
1552
16803260
2164
166710094
11941
7901250
974
1115
779
5533
4533
446748
44
170
202
317
230
2392
123317
19333
2219
0
34CV
J-2-5-3-1-3times
Togo-II
142216072638
1889
1012
4163
11061
5412
261
377
891
510
6167
4967
4533
5222
207
225
325
252
23590
22860
20278
22243
35CV
J-2-5-3-1-3times
99HS-18
1435
1600
2700
1912
3056
3237
7089
4461449
301
658
469
5700
44673800
4656
148
178
225
183
22444
20240
1696
719884
361305times(77371times
BSEC
TCP
-1)
1499
1767
2058
1774
1882
4665
7920
4822
356
426
724
502
566742673800
4578
271
242
305
273
22011
19295
16417
19241
371305times96AC
-93
16781605
2757
2014
1516
10037
11960
7838
311
11381284
911
560040
673400
4356
182
276
251
236
2396
519625
15800
1979
738
1305timesTo
go-II
1177
16802249
1702
902
4676
9388
4989
241
456
795
497
6533
5533
4733
5600
214
266
296
259
21894
20217
19222
20444
391305times99HS-18
1774
1767
2766
2102
2145
4628
10618
5797
301
447
991
580
5933
4500
3733
4722
128
142
180
150
2275
719413
16967
1971
2
40(77371times
BSEC
TCP
-1)times
96AC
-93
1748
1795
31712238
1537
12586
14379
9501
211
1104
1183
833
546744
00346744
44
127
201
226
185
23294
21089
17858
2074
7
10 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(
days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
41(77371times
BSEC
TCP
-1)times
Togo-II
19652007
2653
2208
2136
5601
9503
5747
315
635
1001
650
5533
4833
410048
22
213
241
286
247
22662
22094
17667
20808
42(77371times
BSEC
TCP
-1)times
99HS-18
15011687
2705
1964
1935
7253
11806
6998
314
704
1067
695
5467
4433
5133
5011
177
167
175
173
22305
19277
16392
19325
4396AC
-93timesTo
go-II1759
1618
2123
1834
1539
10347
12212
8033
325
907
1090
774
5900
450039674789
214
269
339
274
23252
21554
16875
20560
4496AC
-93times
99HS-18
1353
1618
2925
1966
3532
7223
11950
7569
440
654
1024
706
5600
4900
4533
5011
149
175
206
177
24494
20698
18111
2110
145
Togo-IItimes
99HS-18
1117
1278
2582
1659
3448
6764
11910
7374
477
767
1207
817
6000
5133
4333
5156
183
203
216
201
17683
15541
14333
15853
Mean
1513
1618
2492
1874
1656
5773
9854
5761
272
538
885
565
586846
534116
4879
218
233
268
240
21297
19547
16529
1912
4SE
101
073
098
mdash079
152
317
mdash014
014
028
mdash351
365
428
mdash036
039
046
mdash985
1173
1211
mdashCD
284
205
276
mdash221
427
890
mdash038
040
079
mdash12
513
015
2mdash
013
014
016
mdash351
418
431
mdash
(c)
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
1642
1667
1744
1684
1504
1349
1526
1460
2783
2405
2634
2607
836
745
908
830
4287
3754
4160
4067
2H7729-2
1693
1817
1950
1820
1012
859
1016
962
3433
2809
2591
2944
649
597
651
632
4445
3668
3607
3906
3G73-107
2000
2028
2077
2035
2280
1966
2312
2186
4695
4023
4229
4316
1281
1170
1463
1305
6975
5989
6541
6502
477245
1523
1630
1728
1627
1796
2489
2702
2329
5423
4851
4498
4924
748
1464
1743
1319
7219
7341
7200
7253
5CV
J-2-5-3-1-3
2282
2692
2787
2587
2411
2643
2901
2652
5857
4836
5024
5239
1206
1335
1769
1437
8268
7479
7925
7891
61305
1851
1951
2054
1952
2026
1763
1908
1899
4657
4414
4463
4511
1468
1336
1539
1448
6683
6177
6371
6410
777371timesBS
ECTCP
-11629
1645
1733
1669
1244
1692
1884
1607
4925
4183
4435
4515
625
1092
1483
1067
6169
5875
6319
6121
896AC
-93
1379
1519
1600
1499
2066
2942
3088
2698
4501
4095
4229
4275
984
1565
1678
1409
6567
7037
7316
6973
9To
go-II
1904
2057
2103
2021
2167
1844
2358
2123
4520
3446
3904
3957
1298
1138
1502
1313
6687
5290
6262
6080
1099HS-18
1475
1485
1633
1531
3180
2904
3382
3155
5633
4139
5205
4992
1656
1536
1879
1690
8813
7043
8587
8147
1H77833-2timesH7729-2
2010
2046
2133
2063
3645
3075
4377
3699
7675
6540
7199
7138
2014
1708
2880
2201
11320
9615
11576
10837
2H77833-2timesG73-107
2066
2101
2122
2096
5002
4053
4601
4552
7882
7430
6963
7425
3146
2565
2808
2840
12884
11482
11564
11977
3H77833-2times77245
1764
2048
2083
1965
3769
4497
4559
4275
8681
8515
8475
8557
2284
3356
3377
3006
12450
13012
13034
12832
4H77833-2times
CVJ-2-5-3-1-3
2205
2383
2733
2441
3693
5655
5220
4856
11656
10287
1010
510683
2198
4488
4176
3621
15348
1594
215325
15538
5H77833-2times1305
2209
2423
2491
2374
6008
5842
6933
6261
15238
14311
13889
14479
3758
4057
5591
4469
21246
2015
320822
2074
0
6H77833-2times(77371times
BSEC
TCP
-1)
1789
1823
1992
1868
3593
4288
4903
4262
8922
6522
7429
7625
2178
3224
3070
2824
12515
10811
12333
11886
7H77833-2times96AC
-93
1739
1960
2011
1903
4557
4350
4818
4575
7916
6068
7290
7092
2411
2529
3191
2710
12474
10419
1210
811667
8H77833-2timesTo
go-II
1927
2389
2406
2241
4185
5828
6215
5409
12319
10279
11023
11207
2433
4254
4631
3773
16504
1610
717238
16616
9H77833-2times99HS-18
1941
2245
2330
2172
4227
4139
4529
4298
9211
8120
7969
8433
3154
3184
3253
3197
13438
12259
12498
1273
210
H7729-2timesG73-107
1968
2109
2158
2079
3725
3042
3488
3418
7313
5956
6174
6481
2822
2001
2357
2393
11038
8998
9662
9899
Advances in Agriculture 11
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
11H7729-2times77245
1847
2048
2200
2032
2467
3759
5466
3898
7672
7449
8921
8014
1356
2350
3796
2500
1013
911208
14387
11911
12H7729-2times
CVJ-2-5-3-1-3
2218
2585
2767
2523
3674
4372
4483
4176
10053
9467
8664
9395
2417
3312
3345
3025
1372
813839
1314
713571
13H7729-2times1305
2169
2338
2547
2352
6728
6166
6792
6562
13333
11662
13301
1276
54128
4501
5660
4763
20061
17828
20093
19327
14H7729-2times(77371times
BSEC
TCP
-1)
1925
2095
2127
2049
3530
4165
4649
4115
9638
6917
7698
8084
1681
2833
3150
2555
1316
8110
82
12347
1219
915
H7729-2times96AC
-93
2012
2070
2180
2087
4955
4447
4596
4666
8738
6882
7762
7794
3754
3558
3181
3498
13693
11329
12359
12460
16H7729-2timesTo
go-II
2138
2428
2563
2377
4949
4660
5118
4909
8549
7243
7191
7661
3586
3353
3028
3322
13498
11903
12310
12570
17H7729-2times99HS-18
1899
1933
2073
1969
4046
4527
4988
4520
8492
7895
8144
8177
2561
3378
3553
3164
12539
12422
1313
212698
18G73-107times77245
1840
2253
2437
2177
2580
4776
5915
4424
11673
10608
10459
10913
1358
3163
4286
2936
14253
15385
16374
15337
19G73-107times
CVJ-2-5-3-1-3
2324
2537
2691
2517
4958
5030
5599
5196
9524
9395
1114
610022
3241
3645
4444
3776
14483
14425
1674
515217
20G73-107times1305
2228
2433
2583
2415
6340
5794
6373
6169
12490
1015
8119
61
11536
4199
4199
5018
4472
18830
1595
218334
1770
6
21G73-107times(77371times
BSEC
TCP
-1)
1943
2128
2220
2097
3936
3980
5770
4562
11697
9858
10303
10619
1874
2369
4181
2808
15633
13839
16073
1518
222
G73-107times96AC
-93
2118
2218
2317
2217
4270
5843
6201
5438
10319
9872
1015
910117
2703
4460
4379
3847
14589
15715
16359
15555
23G73-107timesTo
go-II
2237
2365
2733
2445
5736
5175
6822
5911
12098
10808
1299
611967
3984
3670
5372
4342
17835
1598
219818
17878
24G73-107times99HS-18
2025
2081
2322
2143
3558
4229
6612
4800
12350
9685
11516
1118
42196
2782
5009
3329
1590
813914
1812
915983
2577245timesCV
J-2-5-3-1-3
2109
2360
2675
2381
6760
6558
7299
6872
15225
11861
1416
21374
94225
4752
5839
4939
2198
518418
21461
20621
2677245times1305
1919
2100
2197
2072
5700
5142
5347
5397
11687
10565
10737
10996
4318
3956
4244
4173
17386
1570
816085
16393
2777245times(77371times
BSEC
TCP
-1)
1851
1935
2020
1936
2577
2460
2838
2625
6989
4778
4636
5468
1631
1662
1999
1764
9566
7238
7474
8093
2877245times96AC
-93
1729
1780
2133
1881
5541
5058
5748
5449
11952
8174
9526
9884
3646
3587
4071
3768
17493
13232
15274
15333
2977245timesTo
go-II
2300
2508
2658
2489
3067
5431
5963
4820
11737
11021
1216
411641
1598
4053
4770
3474
14805
16452
1812
616461
3077245times99HS-18
1982
2019
2223
2075
3846
3119
4225
3730
7493
6720
6476
6896
3205
2578
2643
2809
11339
9839
1070
110626
12 Advances in Agriculture
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
31CV
J-2-5-3-1-3times1305
2406
2553
3067
2675
4178
4796
6281
5085
13822
11425
12252
12500
2472
3241
4907
3540
18000
16222
18532
17585
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
2142
2173
2238
2184
4522
5235
5640
5132
11673
9658
10248
10526
2614
3766
4178
3519
1619
514893
15888
15659
33CV
J-2-5-3-1-3times
96AC
-93
2350
2477
2600
2476
4676
4280
6090
5015
11975
9832
10868
10891
3097
2931
4445
3491
16651
14111
1695
815907
34CV
J-2-5-3-1-3times
Togo-II
2093
2666
2989
2583
4058
3727
5190
4325
12588
10717
11217
11507
2742
2570
4185
3166
16645
14443
16407
15832
35CV
J-2-5-3-1-3times
99HS-18
2028
2311
2386
2242
3287
2513
5356
3719
10055
9733
1036
410051
2004
1581
4251
2612
13342
12246
1572
01376
9
361305times(77371times
BSEC
TCP
-1)
2005
2173
2210
2129
3569
4692
5140
4467
11652
9826
10041
10507
2005
3665
4179
3283
15221
14518
1518
214974
371305times96AC
-93
1822
2003
2047
1957
5115
4023
4813
4650
9965
7675
7571
8404
3456
2647
3155
3086
15080
11698
12384
13054
381305timesTo
go-II
2545
2583
2644
2591
6885
7283
6795
6987
12265
11410
12583
12086
4500
6120
5350
5323
1915
018693
19378
19074
391305times99HS-18
2070
2408
2593
2357
5219
4332
4800
4784
1092
97677
8968
9192
3924
3332
3840
3699
1614
912009
1376
913976
40(77371times
BSEC
TCP
-1)
times96AC
-93
2056
2115
2157
2109
3158
4463
4717
4113
9166
7540
8100
8268
1880
3542
3347
2923
12324
12003
12817
12381
41(77371times
BSEC
TCP
-1)
timesTo
go-II
1868
2253
2450
2190
4422
6458
7038
5973
1415
312369
1379
713439
2291
4646
5586
4174
18575
18827
20835
19412
42(77371times
BSEC
TCP
-1)
times99HS-18
1995
2026
2209
2077
2720
2909
3543
3057
7392
4692
5023
5702
1766
2187
2147
2033
10112
7601
8566
8760
4396AC
-93timesTo
go-II
1953
2027
2511
2164
5972
4391
7126
5830
15537
1392
714432
14632
3780
2538
5655
3991
21510
18318
21558
20462
4496AC
-93times99HS-18
1952
2021
2242
2072
3465
2758
4496
3573
5959
5186
6094
5746
2707
2154
2409
2423
9424
7944
10590
9319
45To
go-IItimes
99HS-18
1932
2008
2658
2199
5381
4995
5436
5270
10299
8876
9244
9473
3986
3842
3668
3832
15680
13871
14680
1474
3Mean
1982
2146
2301
2143
3963
4123
4836
4307
9498
8087
8663
8749
2510
2914
3513
2979
13460
12210
13499
13056
SE243
198
245
mdash671
501
626
mdash959
750
822
mdash423
349
344
mdash1155
927
1167
mdashCD
087
071
087
mdash239
178
223
mdash342
267
293
mdash15
112
412
2mdash
411
330
416
mdash
Advances in Agriculture 13Ta
ble4Analysis
ofvaria
ncefor
10times10
half-diallelfor
different
charactersin
threee
nviro
nments
(a)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Germination
()
Rateof
emergence
STI()
SSTI
()
Num
bero
fleavesseedling
Seedlin
gheight
(cm)
Freshweight
(g)seedlin
gDry
weight
(g)seedlin
g
E 1
Replicates
2652731
596768
783393
20700
93018674
33764
1033999
002590
Treatm
ents
541097
0255lowastlowast
8181153lowastlowast
30863092lowastlowast
20680900lowastlowast
1100708lowastlowast
2106317lowastlowast
930829527lowastlowast
319767lowastlowast
Parents
95118
163lowastlowast
14289925lowastlowast
59114816lowastlowast
18880254lowastlowast
1084227lowastlowast
1087906lowastlowast
22561719lowastlowast
042162lowastlowast
Hybrid
s44
11423573lowastlowast
3398933lowastlowast
25697199lowastlowast
20633716lowastlowast
472874lowastlowast
1391526lowastlowast
674123518lowastlowast
261839lowastlowast
Parentsv
ersush
ybrid
s1
43693104lowastlowast
163619
916lowastlowast
3896838
3896
2793lowastlowast
28873741lowastlowast
4272
2798lowastlowast
234144289lowastlowast
5367022lowastlowast
Error
108
281184
1356832
1173684
674337
040104
306
462
2015
1619
005535
E 2
Replicates
2583819
3707389lowast
1596635
198738
034925
448213
899529
007177
Treatm
ents
549997443lowastlowast
2825177lowastlowast
35378177lowastlowast
19074306lowastlowast
665284lowastlowast
1764
081lowastlowast
257694570lowastlowast
2177519lowastlowast
Parents
96507784lowastlowast
3718
879lowastlowast
69866289lowastlowast
1798
5404lowastlowast
705379lowastlowast
1408518lowastlowast
10612519lowastlowast
077429lowastlowast
Hybrid
s44
10569731lowastlowast
2127302lowastlowast
2910
2537lowastlowast
19429240lowastlowast
329256lowastlowast
7000
03lowastlowast
186026552lowastlowast
1483428lowastlowast
Parentsv
ersush
ybrid
s1
16223710lowastlowast
25488361lowastlowast
1113
326
13257362lowastlowast
15089673lowastlowast
5178
3569lowastlowast
5634825791lowastlowast
51618348lowastlowast
Error
108
334760
1097039
1049505
635570
06764
116
0576
694613
006206
E 3
Replicates
2894398
4808815lowast
mdashmdash
051076
095989
3768421
030220
Treatm
ents
541261560
0lowastlowast
4842216lowastlowast
mdashmdash
2811077lowastlowast
5947316lowastlowast
3870844
50lowastlowast
3445076lowastlowast
Parents
97135
735lowastlowast
1097
6388lowastlowast
mdashmdash
1468699lowastlowast
1112
764lowastlowast
136081650lowastlowast
1307836lowastlowast
Hybrid
s44
13217740lowastlowast
1379360
mdashmdash
15540
71lowastlowast
4581632lowastlowast
252084767lowastlowast
2378032lowastlowast
Parentsv
ersush
ybrid
s1
35440
180lowastlowast
102000335lowastlowast
mdashmdash
70200767lowastlowast
109548362lowastlowast
8586095705lowastlowast
69630188lowastlowast
Error
108
782907
1085444
mdashmdash
139771
291325
3019
169
02400
4(b)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Panicle
emergence
Effectiv
etillersplant
Planth
eight
Earlength
Earw
eightp
lant
Dry
fodd
eryieldplant
Grain
yieldplant
Totalbiological
yieldplant
E 1
Replicates
231860
61lowastlowast
416348lowastlowast
1672
9472lowast
1706
411077192
9634539
392777
115544
59Treatm
ents
544152
368lowastlowast
085657lowastlowast
326706089lowastlowast
1602572lowastlowast
6177
0406lowastlowast
304113
204lowastlowast
34285030lowastlowast
575617
582lowastlowast
Parents
94511481lowastlowast
018538lowastlowast
140807476lowastlowast
2240093lowastlowast
11653
959lowastlowast
27014155lowastlowast
3858650lowastlowast
61321919lowastlowast
Hybrid
s44
3201347lowastlowast
100797lowastlowast
110395657lowastlowast
1010
497lowastlowast
40296671lowastlowast
171271411lowastlowast
2413
6880lowastlowast
302999650lowastlowast
Parentsv
ersush
ybrid
s1
4276
5286lowastlowast
02360
9lowast11517452
616lowastlowast
2191
6167lowastlowast
1457662749lowastlowast
8643043524lowastlowast
7546
41053lowastlowast
17199467546lowastlowast
Error
108
468159
004907
3692099
224610
17114
303498126
682350
5075268
E 2
Replicates
2656364
002556
61575565lowastlowast
828842lowastlowast
589094
2268506
260877
1507116
Treatm
ents
542999618lowastlowast
069822lowastlowast
268998723lowastlowast
2436886lowastlowast
60592784lowastlowast
247259877lowastlowast
4192
0073lowastlowast
513483489lowastlowast
Parents
926300
00lowastlowast
048079lowastlowast
165555
405lowastlowast
3871874lowastlowast
1412
2393lowastlowast
19543682lowastlowast
3132
426lowastlowast
57230706lowastlowast
Hybrid
s44
2533
165lowastlowast
07344
9lowastlowast
9616
2857lowastlowast
1463359lowastlowast
35495366lowastlowast
154759288lowastlowast
26262465lowastlowast
293492304lowastlowast
Parentsv
ersush
ybrid
s1
26850135lowastlowast
105929lowastlowast
880476
6697lowastlowast
32357161lowastlowast
1583112746lowastlowast
6366731560lowastlowast
107994
3646lowastlowast
14299370699lowastlowast
Error
108
507598
005829
5234122
149406
955133
2142411
462311
3273886
E 3
Replicates
2029697
021161
9459847
143746
985141
46800
6492
6334
3345160
Treatm
ents
548045499lowastlowast
094511lowastlowast
192420108lowastlowast
3279225lowastlowast
72049211lowastlowast
298953
021lowastlowast
55061308lowastlowast
651732994lowastlowast
Parents
98010
741lowastlowast
094166lowastlowast
116037343lowastlowast
3702185lowastlowast
16305190lowastlowast
2323644
0lowastlowast
4553
893lowastlowast
71484982lowastlowast
Hybrid
s44
6254377lowastlowast
095514lowastlowast
7378
1513lowastlowast
2188348lowastlowast
31813193lowastlowast
190263916lowastlowast
31572179lowastlowast
368689934lowastlowast
Parentsv
ersush
ybrid
s1
8716
7710lowastlowast
053496lowast
609996
3199lowastlowast
47471212lowastlowast
2344
130203lowastlowast
756272
2861lowastlowast
154314
9707lowastlowast
18327859
719lowastlowast
Error
108
696981
007883
5578505
228608
1491014
2574
223
449588
5187705
lowast
Sign
ificant
at119875=005
lowastlowastSign
ificant
at119875=001
14 Advances in Agriculture
Table 5 Mean performance of the genotypes for membrane thermostability in two environments
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594
Advances in Agriculture 15
Table 5 Continued
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434
Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash
lowastValues are transformed values
0
6
12
18
24
30
36
42
48
54
minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity
G73-10796AC-93CVJ-2-5-3-1-3
Togo-II99HS-18
H77833-2H7729-2772451305
H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305
1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305
H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305
77245 times 1305
77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305
77371 times BSECT CP-1
H77833-2 times G73-107
Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits
moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling
survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The
16 Advances in Agriculture
Table6Po
oled
correla
tioncoeffi
cientamon
gvario
uscharactersin
threee
nviro
nments
Characters
Germination
Rateof
emergence
STI
SSTI
Leaves
seedlin
gSeedlin
gheight
Fresh
weight
Dry
weight
MTS
Panicle
emergence
Effectiv
etillersplant
Plant
height
Earlength
Ear
weightp
lant
Dry
fodd
eryield
plant
Grain
yield
plant
Total
biological
yield
plant
Germination
1000
0357lowastlowast
0353lowastlowast
0590lowastlowast
0230
0373lowastlowast
0293lowast
0260
0276lowastminus0135minus0478lowastlowast
0326lowast
minus0069minus0104
minus0190
minus0145
minus0167
Rateof
emergence
1000
0233
0351lowastlowast
0605lowastlowast
0585lowastlowast
0551lowastlowast
0524lowastlowast
0139minus0593lowastlowast
0032
0517lowastlowast
0217
0388lowastlowast
0361lowastlowast
0348lowastlowast
0377lowastlowast
STI
1000
0837lowastlowast
0241
0129
0081
004
80890lowastlowast
minus0215
minus0211
0030minus0007minus0043
minus0101
minus0111
minus0084
SSTI
1000
0233
0264
0183
0140
0745lowastlowast
minus0247minus0268lowast
0184minus0017minus006
6minus0129
minus0144
minus0112
Num
bero
fleaves
1000
0683lowastlowast
0633lowastlowast
060
4lowastlowast
0176minus0474lowastlowast
0109
0576lowastlowast
0496lowastlowast
064
1lowastlowast
0607lowastlowast
0630lowastlowast
0630lowastlowast
Seedlin
gheight
1000
0703lowastlowast
0663lowastlowast
0031minus0493lowastlowast
minus0115
0735lowastlowast
0360lowastlowast
0526lowastlowast
0520lowastlowast
0515lowastlowast
0532lowastlowast
Freshweight
1000
0957lowastlowast
minus0016
0425lowastlowast
minus0082
0661lowastlowast
0351lowastlowast
0581lowastlowast
0496lowastlowast
0576lowastlowast
0533lowastlowast
Dry
weight
1000minus0033minus0428lowastlowast
minus0016
0634lowastlowast
0354lowastlowast
0620lowastlowast
0509lowastlowast
0614lowastlowast
0554lowastlowast
MTS
1000minus040
minus0177minus0037
0015
minus0074
minus0138
minus0131
minus0120
Panicle
emergence
1000
minus0150minus0269lowast
0022minus0329lowast
minus0337lowast
minus0280lowast
minus0341lowast
Effectiv
etillersplant
1000
minus0012
0170
0366lowastlowast
040
5lowastlowast
0323lowast
0401lowastlowast
Planth
eight
1000
0650lowastlowast
0603lowastlowast
0596lowastlowast
0619lowastlowast
0610lowastlowast
Earlength
1000
0608lowastlowast
0671lowastlowast
064
8lowastlowast
066
4lowastlowast
Earw
eightp
lant
1000
0910lowastlowast
0984lowastlowast
0957lowastlowast
Dry
fodd
eryield
plant
1000
0907lowastlowast
0991lowastlowast
Grain
yield
plant
1000
0950lowastlowast
Totalbiological
yield
plant
1000
lowastSign
ificant
at119875=005lowastlowastSign
ificant
at119875=001
Advances in Agriculture 17
faster germination and early vigour in seedling could act asphysical indicators for heat tolerance
Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits
Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics
Conflict of Interests
The authors declare that there is no conflict of interestsregarding the publication of this paper
References
[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981
[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993
[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006
[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993
[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013
[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007
[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965
[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011
[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001
[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009
[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962
[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967
[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963
[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001
Submit your manuscripts athttpwwwhindawicom
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Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
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International Journal of
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International Journal of
Microbiology
The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom
Applied ampEnvironmentalSoil Science
Volume 2014
AgricultureAdvances in
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PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
BiodiversityInternational Journal of
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GenomicsInternational Journal of
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Plant GenomicsInternational Journal of
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2 Advances in Agriculture
Table 1 Description of pearl millet genotypes used as parents for hybridization
Sr No Genotype Response Status Origin and characteristics
1 H77833-2 Tolerant Inbred Bred at CCSHAU Hisar by selfing and selection within a Rajasthanilandrace population
2 H7729-2 Susceptible InbredBred at CCSHAU Hisar It is medium dwarf nodalpigmentationmdashviolet brown resistant to downy mildew shoot flygrey weevil chaffer beetle and leaf roller
3 G73-107 Tolerant Inbred Bred at CCSHAU Hisar and has profuse tillering narrow leaves andtypical orange brown anthers
4 77245 Susceptible Inbred Bred at CCSHAU Hisar by selfing and selection It is late floweringand high tillering
5 CVJ-2-5-3-1-3 Tolerant Inbred Dwarf developed at ICRISAT Patancheru
6 1305 Susceptible Inbred Bred at CCSHAU Hisar late flowering medium dwarf semicompactearhead
7 (77371 times BSECT CP-1) Tolerant Inbred Bred at CCSHAU Hisar using BSECT CP-1 from ICRISATPatancheru as male parent
8 96AC-93 Tolerant Inbred Resistant to downy mildew bred at CCSHAU Hisar by selfing andselection
9 Togo-II Tolerant Inbred Selection from ICRISAT material-Togo bred at CCSHAU Hisar10 99HS-18 Tolerant Inbred Bred at CCS Haryana Agricultural University Hisar
Table 2 Details of different environments created through sowing dates
Environment Nature of environment Date of sowing Site Location
Environment-1 (E1) Heat stress May 21 Research area Department ofGenetics and Plant Breeding
CCS HAU Hisar Haryana
Environment-2 (E2) Heat stress June 13 Research area Department ofGenetics and Plant Breeding
CCS HAU Hisar Haryana
Environment-3 (E3)(during monsoon season)
Nonstress July 17 Research area Department ofGenetics and Plant Breeding
CCS HAU Hisar Haryana
germination and early seedling stages in pearl millet and toidentify some promising F
1hybrids improvement for this
trait particularly for commercial cultivation
2 Materials and Methods
21 Plant Material A field screening technique that isthermotolerance index [4] was applied to screen the pearlmillet F
1hybrids for heat tolerance at seedling stage This
technique could be used in the hottest months (May-June)only with no rainfall Since the technique was applicableto the very young seedlings of pearl millet it was also ofconcern to test the association of seedling thermotoleranceto other phenotypic characteristics of seedlings The mem-brane thermostability (based on quantification of electrolyteleakage) was also conducted for screening the pearl milletF1hybrids at seedling stage Ten pearl millet genotypes
namely H77833-2 H7729-2 G73-107 77245 CVJ-2-5-3-1-3 1305 77371 times BSECT CP-1 96AC-93 Togo-II and 99HS-18 were selected from the germplasm on the basis of differentresponse to supraoptimal temperature tolerance (Table 1) inseparate experiment [5] These were crossed in a half-diallelmating system (excluding reciprocals) The 45 F
1hybrids
produced were tested along with parents for heat toleranceand related traits at seedling stage
22 Experimental Site and Conditions of Growth The exper-iment was conducted at the research area of Departmentof Genetics and Plant Breeding Chaudhary Charan SinghHaryana Agricultural University Hisar (Lat 29∘ 101015840N Long75∘ 461015840E and 2152m above mean sea level) located insubtropical region of Haryana India The 45 F
1hybrids
produced were tested along with parents for heat toleranceand related traits in three replicated plots under supraop-timal temperature exposure at seedling stage in two stressenvironments (environment-1 and environment-2) createdthrough different dates of sowing on May 21 and June 13 (thehottest period in North India) respectively so as to conductthe experiment under different range of temperatures withno rainfall A presowing medium light irrigation was donethrough flooding and the field was precisely levelled beforesowing After sowing no further irrigation was given to thestressed environments (environment-1 and environment-2)One nonstress environment (Table 2) was created throughsowing on July 17 (normal sowing period during monsoon)Each genotype was grown in 3 rows each of 3m length spaced
Advances in Agriculture 3
0
10
20
30
40
50
60
70
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15DAS
E1
E2
E3
Tem
pera
ture
(∘C)
(a)
05
101520253035404550
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15DAS
E1
E2
E3
Tem
pera
ture
(∘C)
(b)
0
10
20
30
40
50
60
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16DAS
E1
E2
E3
Tem
pera
ture
(∘C)
(c)
02468
101214161820
1 3 5 7 9 11 13 15
Moi
sture
()
DAS
E1
E2
E3
Permanent wilting point
(d)
Figure 1 (a) Soil surface temperature (b) air temperature (c) temperature 5 cm above the soil surface and (d) soil moisture status in differentenvironments
30 cm apart with 10 cm plant to plant spacingThe seeds weresown manually
The soil of experimental field was sandy loam in textureThe water holding capacity field capacity and permanentwilting point of the experimental field were 40 17 and 6percent respectively [6] Irrigation was withheld during thecourse of experiment however drought stress-free condi-tions were ensured through monitoring at regular interval(on alternate days) by measuring the moisture status of thesoil by gravimetric method The soil samples of about 50 gwere taken up to 20 cmdepth from the experimental plotThemoisture content on dry weight basis may be calculated [7]using the following formula
Soil moisture ()
=
(Weight of wet soil minusWeight of dry soil)Weight of dry soil
times 100
(1)
The heat stress period was terminated by irrigating theexperimental field before reaching the soil moisture level atpermanent wilting point A condition of no drought wasmaintained in order to determine the exclusive effect of tem-perature at seedling stageThis was maintained by measuringthe moisture status of the soil by gravimetric method onalternate days The soil surface temperature was measuredby a soil thermometer at soil surface temperature at 5 cmabove the soil surface and air temperature at 1m above thesoil surface were recorded between 200 pm and 230 pmdaily (Figure 1)
Emphasis of observation is centred on germination sur-vival and mortality of the seedlings
23 Plant CharacteristicsScreening Techniques The empha-sis was given to the observations of germination survival andmortality of the seedlingsThe seedlings were inspected everyday in the morning The seedlings which had died due to
4 Advances in Agriculture
heat stress were noted in each genotype on daily basis Obser-vations continued till the surviving seedlingswere established(15 DAE) and there was no further mortality Moreover thesoil moisture was depleting near to the permanent wiltingpoint and drought stress could also come into play along withheat stress to further continue the experiment Since experi-ment proceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedlingsurvival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of heat stress at seedling stage was observed on latergrowth stages
231 Thermotolerance Characteristics For heat tolerancecharacteristics 3 indices namely seedling thermotoleranceindex (STI) [4] seed to seedling thermotolerance index(SSTI) [8] and membrane thermostability (MTS) wereapplied to evaluate the hybrids and parents STI was cal-culated as the ratio of number of seedlings survived to thenumber of seedling emergences expressed in percentageSeed to seedling thermotolerance index (SSTI) was calculatedas the ratio of seedling survival to the number of seedlingsexpected to emerge expressed in percentage [8] The SSTIis an extension of STI by taking expected germination intoaccount It was necessary to correct the effect of under soilmortality (USM) The loss of germination due to heat (ieunder soil mortality) can be measured only after a standardgermination test Therefore the germination of seed fromthe same lot under monsoon environment (main pearl milletgrowing season where no under soil mortality occurreddue to heat stress) was taken as expected germination forcalculation of SSTI
232 Membrane Thermostability These genotypes were alsoscreened through laboratory technique (membrane ther-mostability) [9 10] The young leaves of 21-day-old seedlingsof each genotype in three replication plots were used formembrane thermostability test A middle portion of 10 cmfrom each leaf was taken and the midrib was removed andtwo equal halves of the 10 cm each were made Each halfwas taken to provide replications Each leaf portion was cutinto pieces and thoroughly washed and rinsed with distilledwater two times to remove the electrolytes adhering to theplant tissue as well as electrolyte released from the cutting ofplant tissue and put them into test tubes After final rinsingtubes were drained maintaining sufficient water to preventdesiccation of plant material during heat treatment Thesetest tubes were covered with aluminium foil and incubated inwater bath at 55∘C for 15 minutes for giving heat treatmentAfter the heat treatment period 10mL of distilled waterwas added to the test tubes Then all the test tubes wereheld at 10∘C overnight to allow diffusion of electrolytes fromplant material Test tubes were brought out of refrigeratorand shaken well to mix the contents An initial electricalconductivity (EC) of both replications of each treatment (119879
1)
was determined with electrical conductivity meter Thenthese test tubes were autoclaved at 15 psi for 2 minutes forcomplete electrolyte leakage from the leaf samples These testtubes were then cooled at room temperature and contentsmixed and final EC was taken for all the treatments (119879
2)
Membrane thermostability (MTS) was calculated accordingto Ibrahim and Quick [9] and Yadav et al [10] as follows
Membrane Thermostability (MTS) = (1 minus1198791
1198792
) times 100
(2)
where 1198791is conductivity reading after heat treatment and 119879
2
is conductivity reading after autoclavingCompletely randomized design (CRD) for membrane
thermostabilitywas conducted to detect variations among thegenotypes
233 Morphological Characteristics The seedling growthcharacteristics namely germination () emergence rate(ER) number of leavesseedling (two-week stage) seedlingheight (two-week stage) seedling fresh weight (g) (four-week stage) and seedling dry weight (g) (four-week stage)were recorded The data for seedling height and number ofleaves per seedling was recorded on 10 random seedlings perplot Emergence rate (an index for speed of germination)can be used as a tool in pearl millet breeding programs forevaluation of seedling vigour Speed of germination is oneof the oldest concepts of seedling vigour The emergencerate (ER) was calculated by dividing the number of normalseedlings obtained at each counting by the number of daysseeds had been in the seed bed (DAS)The values obtained ateach count were then summed at the end of the germinationtest to obtain the emergence rate [11]
Emergence Rate (ER) =Seedling count
Days to first count+ sdot sdot sdot
+
Seedling countDays to final count
(3)
After termination of the heat stress on the seedlings byirrigating the field the experiment was continued to studythe effect of seedling heat stress on maturity traits on thesurvived plants The maturity traits observed were panicleemergence effective tillersplant plant height ear length earweightplant dry fodder yieldplant grain yieldplant andtotal biological yieldplant
24 Statistical Analysis Analysis of variance for randomizedblock design (RBD) for field traits that is germinationemergence rate number of leavesseedling seedling heightseedling fresh weight and seedling dry weight and com-pletely randomized design (CRD) for membrane thermosta-bility test was conducted under each environment on meanbasis [12] Analyses were done using the software OPSTATdeveloped at CCS HAU Hisar Correlations among the vari-ous characters studied were calculated to establish the natureand magnitude of associations among the traits [8 13] with
Advances in Agriculture 5
the software SPSS (SPSS Inc) Multivariate clustering wasdone based on paired group cluster method using similaritycoefficient under Past 140 software [14] Multivariate analysisbased on the Euclidean distancematrix was estimated amonggenotypes to distinguish genotypes from each other Thegrouping of genotypes was generated using paired groupcluster method
3 Results and Discussion
The excessive heat (soil temperature up to 630∘C in E2)
about 18∘C higher than the normal atmospheric temperature(Figures 1(a) and 1(b)) was responsible for mortality ofseedlings Many seedlings were found desiccated at the pointof contact besides the desiccation at the tipsThe temperaturearound the tip level (5 cm above the soil surface) also reachedup to 50∘C (Figure 1(c)) It could therefore be concluded thatthe seedlings had to survive in a really high temperature Themoisture level remained above the permanent wilting point(Figure 1(d)) therefore drought effect was not in the playThe heat tolerance was measured as STI (Table 3) throughthe field screening technique of Peacock et al [4] Anotherindex known as SSTI was also calculated as an improvementand extensionThe analysis of variance (RBD) for 8 charactersin 3 environments showed significant genotypic differencesNo variability was observed for STI and SSTI in E
3only In
this environment no seedling mortality was observed andthe germination was taken as standard The values for STIand SSTI were therefore equal and the highest for all thegenotypes in this environment and hence no variation wasobserved
The hybrids were evaluated on the basis of mean perfor-mance for various characters in each environment (Table 3)On the mean basis STI value ranged from 5332 to 8224with an average of 7072 The hybrid H7729-2 times CVJ-2-5-3-1-3 was the best scorer for heat tolerance followed byH77833-2 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II The hybrid H77833-2 times 1305 was found susceptible toheat stress Seed to seedling thermotolerance index (SSTI)which is expected to be more rigorous index deviated thevalues from STI according to variation in germination undernormal environment (E
3) as the SSTI also coversincludes
the under surface mortality (USM) during germinationThe reductions in values were not strictly proportional tothe STI The reduction is dependent on thermosensitivityof germination We also came across such hybrids whichshowed poor germination and high STI SSTI values rangedfrom 4605 to 7287 with an average of 6090 The genotypeH77833-2 times 96AC-93 had the highest worth for SSTI onmean basis followed byG73-107times (77371timesBSECTCP-1) andH77833-2 times (77371 times BSECT CP-1) (Table 3) The analysisof variance for ten different characters in three differentenvironments (two stressed and one normal) was carried outand the results showed significant genotypic differences forall the characters (Table 4)
A caution is needed for evaluating such genotypes underSTI The SSTI gave a highly reduced value putting thesecrosses in the susceptible list It may be concluded therefore
that SSTI is more dependable and desirable tool for screeningand evaluating the genotypes for heat tolerance The germi-nation was lower in E
2than in E
1 This period has the highest
range of temperature with a high average Genotype-wise thehighest germination (6209) was recorded for the genotypeH7729-2 times (77371 times BSECT CP-1) on mean basis whichgives a preliminary indication of resistance to heat while thegenotype 1times 6 had the lowest germination (3533)The hybridH77833-2 times G73-107 was adjudged as the fastest emergenceof seedlingsThe genotype (77371timesBSECTCP-1)times 96AC-93had the highest fresh weightseedlingThe genotype H7729-2 times Togo-II had the highest seedling dry weight E
1had lower
dry weightseedling than E2indicating the more heat stress
during the E1
31 Membrane Thermostability Membrane thermostability(MTS) has long been recognised as a parameter of heattolerance All the hybrids were put to membrane thermosta-bility test in the laboratory under standard protocol Acompletely randomised design (CRD) analysis was carriedout for membrane thermostability (MTS) The mean sum ofsquares due to genotypes was highly significant indicatingthat enough genetic variability was present for membranethermostability (MTS) in each environment which justifiedfurther analysis of the data The range of values lay between5073 and 7742 Among the parents H77833-2 G73-107CVJ-2-5-3-1-3 77371 times BSECT CP-1 96AC-93 Togo-II and99HS-18 were verified as resistant genotypes and H7729-2 77245 and 1305 genotypes were found susceptible asmentioned in Table 1 (on the basis of previous experiment)Among all the hybrids H7729-2 times CVJ-2-5-3-1-3 CVJ-2-5-3-1-3 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II werethree highly tolerant genotypes on the basis of membranethermostability (Table 5)
32 Correlations The pooled correlations among the charac-ters were calculated (Table 6) around heat tolerance indices(STI SSTI and membrane thermostability) with a view tofind whether there was any association of these characterswith seedling as well as maturity traits It was observedthat STI and SSTI recorded the highest significant cor-relation among themselves as they are related indices forheat tolerance Membrane thermostability also showed highsignificant correlation with STI as well as SSTI STI hada significant positive association with germination of seedonly among seedling and maturity traits studied SSTI inaddition was also positively correlated with germination andemergence rate and negatively associated with number ofeffective tillersplantMembrane thermostability also showedsignificant positive correlation with seed germination amongseedling and maturity traits studied While evaluating thediallel progenies it was also seen that the heat toleranceindices STI SSTI and membrane thermostability were notshowing any perceptible correlation with the rest of thedevelopmental traits (seedling andmaturity traits)The stressperiod was terminated by irrigating the field experiment after15 days fromdate of sowingwhen the seedlings get establishedand further mortality stopped due to heat Moreover the soil
6 Advances in Agriculture
Table3Perfo
rmance
ofthep
arentsandthec
rosses
ford
ifferentcharacters
(a)
Sr
No
Genotypes
Germination(
)Ra
teof
emergencelowast
STIlowast
()
SSTIlowast(
)Num
bero
fleavesseedlin
gE 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
3941
3737
6164
4614
4037
4109
4279
4142
7830
7387
9000
8072
4498
4133
9000
5877
661
502
800
654
2H7729-2
5513
4726
6092
5443
2815
4980
3258
3684
4835
4495
9000
6110
4441
3602
9000
5681
363
294
427
361
3G73-107
5037
4826
5918
5260
4534
4132
4481
4382
6187
5413
9000
6866
5213
4460
9000
6224
878
710
935
841
477245
4747
4624
5565
4978
3625
4604
4047
4092
4385
3580
9000
5655
3803
3040
9000
5281
377
292
437
369
5CV
J-2-5-3-1-3
4824
4832
6897
5518
5044
4907
4960
4970
7193
6813
9000
7669
4959
4785
9000
6248
802
580
923
768
61305
4326
3891
5404
4540
3376
5056
3573
4002
3903
3281
9000
5395
3211
2452
9000
4888
350
284
414
349
777371timesBS
ECTCP
-14747
4502
6320
5190
4934
5075
5065
5025
6952
6706
9000
7552
5056
4671
9000
6242
621
542
700
621
896AC
-93
4651
3818
5722
4730
4312
4915
4753
4660
6660
6001
9000
7220
5212
3920
9000
6044
701
590
899
730
9To
go-II
4613
3937
5276
4609
4245
4737
4797
4593
7198
6861
9000
7686
5980
4767
9000
6582
681
629
853
721
1099HS-18
4575
3823
6247
4882
4317
4659
4746
4574
7594
7319
9000
7971
5154
4172
9000
6109
745
554
915
738
1H77833-2timesH7729-2
5787
5295
6733
5938
4964
5015
4989
4989
6803
6504
9000
7436
5808
5134
9000
6647
711
585
780
692
2H77833-2timesG73-107
5472
4417
6405
5431
5448
5422
5239
5370
7231
6769
9000
7667
5980
4556
9000
6512
1101
705
1340
1049
3H77833-2times77245
4076
3707
5504
4429
5217
5446
5238
5300
6627
6224
9000
7284
4690
4037
9000
5909
740
525
965
744
4H77833-2timesCV
J-2-5-3-1-3
4843
4300
5856
5000
4862
4956
4913
4911
7061
6519
9000
7527
5575
4632
9000
6403
998
885
1258
1047
5H77833-2times1305
3296
2780
4521
3533
5076
4907
5238
5074
3732
3265
9000
5332
2765
2050
9000
4605
1192
934
1600
1242
6H77833-2times(77371times
BSEC
TCP
-1)5636
5539
6258
5811
4489
5219
5015
4908
6981
6482
9000
7488
6146
5680
9000
6942
898
726
1525
1050
7H77833-2times96AC
-93
5720
5515
6335
5857
4800
5186
5075
5021
7833
7786
9000
8206
6603
6256
9000
7287
1107
873
1193
1058
8H77833-2timesTo
go-II
4862
3453
5709
4675
5036
4829
4911
4925
6190
5500
9000
6897
5214
3339
9000
5851
979
670
1107
919
9H77833-2times99HS-18
4575
4418
5447
4813
5041
5074
5006
5040
5800
5073
9000
6625
4846
4133
9000
5993
978
733
1135
949
10H7729-2timesG73-107
5615
4962
6273
5617
5023
5535
5165
5241
6143
5501
9000
6881
5540
4441
9000
6327
1000
720
1363
1028
11H7729-2times77245
5472
5164
6281
5639
4745
5031
5028
4935
5572
5175
9000
6582
4901
4345
9000
6082
880
620
897
799
12H7729-2timesCV
J-2-5-3-1-3
5494
5124
6536
5718
4804
5316
4957
5026
7596
8076
9000
8224
6093
5680
9000
6924
981
932
1451
1121
13H7729-2times1305
3804
3826
5511
4380
3596
4724
4387
4236
6362
7075
9000
7479
4230
4517
9000
5916
926
725
1497
1049
14H7729-2times(77371times
BSEC
TCP
-1)
6369
4847
7412
6209
4847
5351
5246
5148
6377
6031
9000
7136
5683
4230
9000
6304
977
671
1380
1009
15H7729-2times96AC
-93
5314
4657
6702
5558
4514
5295
5102
4971
5612
4856
9000
6489
4613
3603
9000
5739
846
679
1397
974
16H7729-2timesTo
go-II
5827
5594
6651
6024
5072
5279
5271
5207
6563
5371
9000
6978
5763
4652
9000
6472
873
719
1040
877
17H7729-2times99HS-18
5741
4638
7918
6099
4847
5024
5021
4964
6179
5603
9000
6927
4882
3725
9000
5869
1012
730
1600
1114
18G73-107times77245
5115
4646
6103
5288
4977
5242
5450
5223
7006
6063
9000
7356
5685
4594
9000
6426
1021
790
1292
1034
19G73-107timesCV
J-2-5-3-1-3
5134
4894
6427
5485
4901
5317
5181
5133
6637
6124
9000
7254
5253
4710
9000
6321
1039
863
1254
1052
20G73-107times1305
4881
4561
7597
5680
4717
4791
4775
4761
6704
6380
9000
7361
4556
4115
9000
5890
1140
882
1657
1226
21G73-107times(77371times
BSEC
TCP
-1)
5636
5519
6283
5812
4986
5219
5203
5136
7990
6203
9000
7731
6620
5436
9000
7019
977
703
1395
1025
22G73-107times96AC
-93
5740
4510
6415
5555
5129
4982
5195
5102
6978
5556
9000
7178
6156
4019
9000
6392
1077
860
1264
1067
23G73-107timesTo
go-II
4460
4357
6044
4953
4396
5138
4951
4829
5279
4835
9000
6371
4018
3603
9000
5540
906
881
1189
992
24G73-107times99HS-18
5473
4356
6374
5401
5102
4955
5055
5037
6460
5197
9000
6886
5534
3704
9000
6079
1171
766
1635
1191
2577245timesCV
J-2-5-3-1-3
4766
4666
5921
5117
5170
4918
4900
4996
5928
5859
9000
6929
4785
4613
9000
6133
984
920
1194
1033
2677245times1305
4575
4060
6052
4895
5128
5297
5203
5210
5102
4990
9000
6364
3979
3464
9000
5481
1279
823
1550
1217
2777245times(77371times
BSEC
TCP
-1)
5017
4929
6022
5322
5108
5473
5274
5285
5308
5044
9000
6451
4498
4191
9000
5896
833
588
939
787
Advances in Agriculture 7
(a)Con
tinued
Sr
No
Genotypes
Germination(
)Ra
teof
emergencelowast
STIlowast
()
SSTIlowast(
)Num
bero
fleavesseedlin
gE 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
2877245times96AC
-93
5472
4705
622154
66
5135
5197
5006
5113
5247
4843
9000
6363
4708
3784
9000
5831
795
662
1058
838
2977245timesTo
go-II
5252
4492
5889
5211
5188
4861
5074
5041
4885
4263
9000
6049
4421
3379
9000
5600
977
751
1243
990
3077245times99HS-18
5740
4099
6872
5570
5249
4898
5258
5135
5628
4616
9000
6415
4863
3014
9000
5626
1045
863
1184
1031
31CV
J-2-5-3-1-3times1305
4364
3944
5700
4669
4974
4517
4650
4714
6097
5579
9000
6892
4594
3842
9000
5812
936
848
1403
1063
32CV
J-2-5-3-1-3times(77371times
BSEC
TCP
-1)4345
3808
6774
4976
5272
4996
5035
5101
6650
5766
9000
7139
4288
3381
9000
5556
800
712
1013
841
33CV
J-2-5-3-1-3times96AC
-93
4862
4197
5743
4934
4884
4980
5058
4974
7477
6760
9000
7745
5957
4709
9000
6555
933
738
1478
1050
34CV
J-2-5-3-1-3timesTo
go-II
4670
4343
6233
5082
4399
4813
4631
4614
5806
6614
9000
7140
4422
4479
9000
5967
963
855
1180
999
35CV
J-2-5-3-1-3times99HS-18
5493
5080
7273
5949
5276
4984
5162
5141
7322
6590
9000
7637
5534
4766
9000
6433
998
807
1338
1047
361305times(77371times
BSEC
TCP
-1)
4613
4539
5717
4956
4986
4955
5080
5007
5137
4870
9000
6336
4210
3936
9000
5715
863
630
913
802
371305times96AC
-93
5473
5177
6830
5827
5224
4779
4919
4974
7117
6965
9000
7694
5640
5220
9000
6620
875
694
1476
1015
381305timesTo
go-II
4230
3420
5262
4304
4309
456144
45
4438
5781
4373
9000
6385
4575
2887
9000
5487
637
566
794
666
391305times99HS-18
5433
4507
6947
5629
5225
4753
4991
4989
7287
6968
9000
7752
5576
4479
9000
6352
1115
818
1410
1114
40(77371times
BSEC
TCP
-1)times
96AC
-93
5595
4429
7461
5828
5216
4955
5121
5097
7087
6368
9000
7485
5432
4038
9000
6157
955
753
1462
1057
41(77371times
BSEC
TCP
-1)times
Togo-II
5252
4728
6106
5362
5492
4808
5132
5144
8025
7505
9000
8177
6310
5413
9000
6908
859
693
990
848
42(77371times
BSEC
TCP
-1)times
99HS-18
5595
4298
6295
5396
4729
4887
5031
4883
7366
6945
9000
7770
6312
4556
9000
6623
903
622
1125
883
4396AC
-93timesTo
go-II
5017
3977
6486
5160
5019
5195
5237
5150
5922
5486
9000
6803
4689
3484
9000
5724
1007
728
1167
967
4496AC
-93times99HS-18
5595
4812
6924
5777
5036
5385
5205
5209
7411
5378
9000
7263
5873
3979
9000
6284
1013
652
1334
1000
45To
go-IItimes
99HS-18
4670
4497
7293
5487
4718
4319
4796
4611
5688
5431
9000
6706
3980
3665
9000
5548
939
653
1462
1018
Mean
5043
4482
6271
5265
4792
498149
23
4899
6377
584190
00
7072
5079
4190
9000
6090
898
701
1168
922
SE097
106
161
mdash213
191
190
mdash19
818
7mdash
mdash14
914
6mdash
mdash036
047
068
mdashCD
272
296
453
mdash597
537
534
mdash555
525
mdashmdash
421
409
mdashmdash
103
133
192
mdashlowastTransfo
rmed
data
(b)
Sr
No
Genotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
908
785
2245
1313
859
1537
1924
1440
154
143
160
152
6267
4800
3833
4967
212
227
249
229
15644
15458
13267
1479
02
H7729-2
1125
1175
1789
1363
317
2400
2811
1843
093
191
252
179
6700
5333
5100
5711
185
210
234
210
18850
18565
15389
17601
3G73-107
1166
1475
1966
1536
943
1744
4945
2544
143
129
366
213
6700
5433
5300
5811
218
219
238
225
15009
1496
513503
14492
477245
1438
1373
2085
1632
607
1863
5957
2809
117
180
509
269
6100
4800
5100
5333
232
221
253
235
14036
13802
11742
1319
35
CVJ-2-5-3-1-3
1214
1437
1807
1486
1163
1610
4166
2313
171
141
407
240
6300
4933
4600
5278
239
227
270
245
1994
317261
1497
017391
61305
858
1137
1737
1244
540
967
4861
2123
125
088
417
210
6533
5033
4800
5456
244
213
257
238
15527
15405
12274
14402
8 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
777371timesBS
ECT
CP-1
1377
1487
1892
1585
928
2660
3330
2306
150
258
301
236
5733
4533
4700
4989
242
220
293
252
1513
814550
1315
014279
896AC
-93
1303
1259
1852
1471
1056
1445
5972
2825
185
147
586
306
5500
4833
3833
4722
256
304
318
293
15577
14032
10000
13203
9To
go-II
1056
1220
2272
1516
924
1466
9113
3834
146
129
883
386
6167
4533
4600
5100
185
149
124
153
14578
12073
10033
12228
1099HS-18
1277
1075
1808
1387
1241
2833
7070
3715
228
226
615
356
6100
5000
4167
5089
243
173
324
247
12627
10355
1017
311052
1H77833-2times
H7729-2
1432
1498
2233
1721
1350
4405
8373
4709
159
499
782
480
5767
4433
3800
4667309
290
248
282
21010
18071
15847
18309
2H77833-2times
G73-107
1675
1635
2205
1838
1236
3211
5789
3412
134
313
547
331
5833
4767
3833
4811
262
295
236
265
21253
20449
15808
1917
0
3H77833-2times
77245
1577
1522
2263
1787
1058
6933
8719
5570
255
553
782
530
5267
4633
4600
4833
273
201
265
246
2074
119511
15208
18487
4H77833-2times
CVJ-2-5-3-1-3
1791
1757
2165
1904
20218030
5276
5109
354
779
477
537
5533
4233
3767
4511
165
330
351
282
21827
20072
17110
19670
5H77833-2times1305
1700
1842
28412128
1427
6228
9134
5596
570
579
813
654
5800
4267
3333
4467340
426
451
406
19816
19444
16037
18432
6H77833-2times
(77371times
BSEC
TCP
-1)
1453
1515
3120
2029
789
465510428
5291
211
408
858
492
5233
4167
3267
4222
203
283
267
251
20677
1913
816470
1876
2
7H77833-2times
96AC
-93
1835
1783
2427
2015
2532
6649
1610
684
29
350
650
1473
824
5467
4233
3700
4467243
239
256
246
22278
20434
15735
19482
8H77833-2times
Togo-II
1564
15632500
1876
1882
4772
11660
6105
296
469
972
579
5667
4533
440048
67
169
260
211
213
2412
82375
619389
22424
9H77833-2times
99HS-18
159318002049
1814
1571
480214024
6799
203
544
1547
765
6033
460040
0048
78
261
225
256
247
23466
21081
1793
320
827
10H7729-2times
G73-107
14951568
2075
1713
959
5157
8517
4877
218
382
666
422
6133
5067
4833
5344
300
273
239
271
23651
2191
717083
20884
11H7729-2times77245
1557
1537
2613
1902
1851
5756
5729
4445366
562
524
484
5633
4800
4633
5022
133
233
297
221
1979
219914
15250
18319
12H7729-2times
CVJ-2-5-3-1-3
18442033
28932257
1651
806114885
8199
264
597
1054
638
6333
4600
4233
5056
170
211
217
199
24629
23339
1970
022556
13H7729-2times1305
1325
1677309920
34
1756
724314048
7682
318
635
1232
728
630046
67
3633
4867298
261
361
307
21666
19371
17422
19486
14H7729-2times(77371
timesBS
ECTCP
-1)
1958
1887
3105
2317
1118
4675
14411
6734
242
457
1318
672
5900
4500
3800
4733
164
209
240
204
23661
1995
419250
20955
15H7729-2times
96AC
-93
1512
1717
2779
2002266844
08
11393
6156
356
500
1211
689
5633
4533
4200
4789
221
195
282
232
2194
921635
17213
20266
Advances in Agriculture 9
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
16H7729-2times
Togo-II
1519
1738
2159
1805
2437
9867
1616
294
89
361
961
1527
950
6167
4700
4200
5022
234
272
230
246
26338
2414
520553
23678
17H7729-2times
99HS-18
1938
1787
3151
2292
1574
6218
11318
6370
290
606
956
617
6067
4767
406749
67
172
210
199
194
24689
21854
1778
921444
18G73-107times77245
1856
1813
2210
1960
2435
8550
7828
6271338
633
612
528
5500
4633
440048
44
199
255
307
254
1993
619277
16095
18436
19G73-107times
CVJ-2-5-3-1-3
1517
1769
2407
1897
1556
7728
9136
6140
239
876
917
677
5900
4300
3400
4533
233
266
315
272
23418
22333
19317
21689
20G73-107times1305
1217
1507
2832
1852
1777
5189
1114
960
38
289
588
1197
691
6733
5000
4233
5322
286
210
312
269
2078
81992
817067
19261
21G73-107times(77371
timesBS
ECTCP
-1)
18551946
2730
2177
1801
3482
10415
5233
239
277
753
423
5500
4467
3433
4467243
197
273
238
23817
20263
17425
20502
22G73-107times
96AC
-93
1718
1840
28612140
1345
9333
14669
8449
297
845
1278
807
5600
5033
4633
5089
270
243
300
271
28537
21461
16875
22291
23G73-107timesTo
go-II
168018083087
2192
1112
4678
9319
5036
186
473
985
548
6033
4767
4200
5000
210
209
231
217
24864
24609
21625
23699
24G73-107times99HS-18
1398
1675
3370
2148
2753
8672
11918
7781
362
691
981
678
566746
00
3500
4589
170
247
270
229
24677
20567
17855
21033
2577245times
CVJ-2-5-3-1-3
1500
1728
2203
1810
2282
4679
11492
6151
328
424
1116
623
6000
4333
420048
44300
231
370
300
21257
20497
1772
519826
2677245times1305
143815772080
1698
949
10439
12053
7814
121
915
992
676
5767
4800
3500
4689
168246
316
244
1978
91779
015517
17699
2777245times(77371times
BSEC
TCP
-1)
150516972846
2016
3222
8518
12621
8120
469
827
1141
812
5400
4367
3833
4533
196
225
242
221
2092
819485
1719
219202
2877245times96AC
-93
1653
1797
2117
1856
1934
6056
11543
6511
352
563
911
608
5333
4267
3833
4478
186
169
198
184
22003
19054
1676
719275
2977245timesTo
go-II
1723
1718
3163
2201
1749
8250
15443
8481229
806
1412
816
5967
4700
4067
4911
174
245
298
239
22075
23503
19875
21818
3077245times99HS-18
2016
1970
28542280
3217
13027
3531
6591395
965
285
549
5733
4467
3833
4678
148
228
209
195
2212
721462
1690
020
163
31CV
J-2-5-3-1-3times
1305
1358
1422
2179
1653
1254
2803
1019
44750
178
254
801
411
6033
4467
3367
4622
312
193
330
278
22269
19547
17250
19689
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
1335
1742
2400
1826
922
5226
12806
6318
236
458
1054
583
5633
4433
3600
4556
321
315
259
298
2210
419524
17087
19572
33CV
J-2-5-3-1-3times
96AC
-93
1552
16803260
2164
166710094
11941
7901250
974
1115
779
5533
4533
446748
44
170
202
317
230
2392
123317
19333
2219
0
34CV
J-2-5-3-1-3times
Togo-II
142216072638
1889
1012
4163
11061
5412
261
377
891
510
6167
4967
4533
5222
207
225
325
252
23590
22860
20278
22243
35CV
J-2-5-3-1-3times
99HS-18
1435
1600
2700
1912
3056
3237
7089
4461449
301
658
469
5700
44673800
4656
148
178
225
183
22444
20240
1696
719884
361305times(77371times
BSEC
TCP
-1)
1499
1767
2058
1774
1882
4665
7920
4822
356
426
724
502
566742673800
4578
271
242
305
273
22011
19295
16417
19241
371305times96AC
-93
16781605
2757
2014
1516
10037
11960
7838
311
11381284
911
560040
673400
4356
182
276
251
236
2396
519625
15800
1979
738
1305timesTo
go-II
1177
16802249
1702
902
4676
9388
4989
241
456
795
497
6533
5533
4733
5600
214
266
296
259
21894
20217
19222
20444
391305times99HS-18
1774
1767
2766
2102
2145
4628
10618
5797
301
447
991
580
5933
4500
3733
4722
128
142
180
150
2275
719413
16967
1971
2
40(77371times
BSEC
TCP
-1)times
96AC
-93
1748
1795
31712238
1537
12586
14379
9501
211
1104
1183
833
546744
00346744
44
127
201
226
185
23294
21089
17858
2074
7
10 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(
days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
41(77371times
BSEC
TCP
-1)times
Togo-II
19652007
2653
2208
2136
5601
9503
5747
315
635
1001
650
5533
4833
410048
22
213
241
286
247
22662
22094
17667
20808
42(77371times
BSEC
TCP
-1)times
99HS-18
15011687
2705
1964
1935
7253
11806
6998
314
704
1067
695
5467
4433
5133
5011
177
167
175
173
22305
19277
16392
19325
4396AC
-93timesTo
go-II1759
1618
2123
1834
1539
10347
12212
8033
325
907
1090
774
5900
450039674789
214
269
339
274
23252
21554
16875
20560
4496AC
-93times
99HS-18
1353
1618
2925
1966
3532
7223
11950
7569
440
654
1024
706
5600
4900
4533
5011
149
175
206
177
24494
20698
18111
2110
145
Togo-IItimes
99HS-18
1117
1278
2582
1659
3448
6764
11910
7374
477
767
1207
817
6000
5133
4333
5156
183
203
216
201
17683
15541
14333
15853
Mean
1513
1618
2492
1874
1656
5773
9854
5761
272
538
885
565
586846
534116
4879
218
233
268
240
21297
19547
16529
1912
4SE
101
073
098
mdash079
152
317
mdash014
014
028
mdash351
365
428
mdash036
039
046
mdash985
1173
1211
mdashCD
284
205
276
mdash221
427
890
mdash038
040
079
mdash12
513
015
2mdash
013
014
016
mdash351
418
431
mdash
(c)
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
1642
1667
1744
1684
1504
1349
1526
1460
2783
2405
2634
2607
836
745
908
830
4287
3754
4160
4067
2H7729-2
1693
1817
1950
1820
1012
859
1016
962
3433
2809
2591
2944
649
597
651
632
4445
3668
3607
3906
3G73-107
2000
2028
2077
2035
2280
1966
2312
2186
4695
4023
4229
4316
1281
1170
1463
1305
6975
5989
6541
6502
477245
1523
1630
1728
1627
1796
2489
2702
2329
5423
4851
4498
4924
748
1464
1743
1319
7219
7341
7200
7253
5CV
J-2-5-3-1-3
2282
2692
2787
2587
2411
2643
2901
2652
5857
4836
5024
5239
1206
1335
1769
1437
8268
7479
7925
7891
61305
1851
1951
2054
1952
2026
1763
1908
1899
4657
4414
4463
4511
1468
1336
1539
1448
6683
6177
6371
6410
777371timesBS
ECTCP
-11629
1645
1733
1669
1244
1692
1884
1607
4925
4183
4435
4515
625
1092
1483
1067
6169
5875
6319
6121
896AC
-93
1379
1519
1600
1499
2066
2942
3088
2698
4501
4095
4229
4275
984
1565
1678
1409
6567
7037
7316
6973
9To
go-II
1904
2057
2103
2021
2167
1844
2358
2123
4520
3446
3904
3957
1298
1138
1502
1313
6687
5290
6262
6080
1099HS-18
1475
1485
1633
1531
3180
2904
3382
3155
5633
4139
5205
4992
1656
1536
1879
1690
8813
7043
8587
8147
1H77833-2timesH7729-2
2010
2046
2133
2063
3645
3075
4377
3699
7675
6540
7199
7138
2014
1708
2880
2201
11320
9615
11576
10837
2H77833-2timesG73-107
2066
2101
2122
2096
5002
4053
4601
4552
7882
7430
6963
7425
3146
2565
2808
2840
12884
11482
11564
11977
3H77833-2times77245
1764
2048
2083
1965
3769
4497
4559
4275
8681
8515
8475
8557
2284
3356
3377
3006
12450
13012
13034
12832
4H77833-2times
CVJ-2-5-3-1-3
2205
2383
2733
2441
3693
5655
5220
4856
11656
10287
1010
510683
2198
4488
4176
3621
15348
1594
215325
15538
5H77833-2times1305
2209
2423
2491
2374
6008
5842
6933
6261
15238
14311
13889
14479
3758
4057
5591
4469
21246
2015
320822
2074
0
6H77833-2times(77371times
BSEC
TCP
-1)
1789
1823
1992
1868
3593
4288
4903
4262
8922
6522
7429
7625
2178
3224
3070
2824
12515
10811
12333
11886
7H77833-2times96AC
-93
1739
1960
2011
1903
4557
4350
4818
4575
7916
6068
7290
7092
2411
2529
3191
2710
12474
10419
1210
811667
8H77833-2timesTo
go-II
1927
2389
2406
2241
4185
5828
6215
5409
12319
10279
11023
11207
2433
4254
4631
3773
16504
1610
717238
16616
9H77833-2times99HS-18
1941
2245
2330
2172
4227
4139
4529
4298
9211
8120
7969
8433
3154
3184
3253
3197
13438
12259
12498
1273
210
H7729-2timesG73-107
1968
2109
2158
2079
3725
3042
3488
3418
7313
5956
6174
6481
2822
2001
2357
2393
11038
8998
9662
9899
Advances in Agriculture 11
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
11H7729-2times77245
1847
2048
2200
2032
2467
3759
5466
3898
7672
7449
8921
8014
1356
2350
3796
2500
1013
911208
14387
11911
12H7729-2times
CVJ-2-5-3-1-3
2218
2585
2767
2523
3674
4372
4483
4176
10053
9467
8664
9395
2417
3312
3345
3025
1372
813839
1314
713571
13H7729-2times1305
2169
2338
2547
2352
6728
6166
6792
6562
13333
11662
13301
1276
54128
4501
5660
4763
20061
17828
20093
19327
14H7729-2times(77371times
BSEC
TCP
-1)
1925
2095
2127
2049
3530
4165
4649
4115
9638
6917
7698
8084
1681
2833
3150
2555
1316
8110
82
12347
1219
915
H7729-2times96AC
-93
2012
2070
2180
2087
4955
4447
4596
4666
8738
6882
7762
7794
3754
3558
3181
3498
13693
11329
12359
12460
16H7729-2timesTo
go-II
2138
2428
2563
2377
4949
4660
5118
4909
8549
7243
7191
7661
3586
3353
3028
3322
13498
11903
12310
12570
17H7729-2times99HS-18
1899
1933
2073
1969
4046
4527
4988
4520
8492
7895
8144
8177
2561
3378
3553
3164
12539
12422
1313
212698
18G73-107times77245
1840
2253
2437
2177
2580
4776
5915
4424
11673
10608
10459
10913
1358
3163
4286
2936
14253
15385
16374
15337
19G73-107times
CVJ-2-5-3-1-3
2324
2537
2691
2517
4958
5030
5599
5196
9524
9395
1114
610022
3241
3645
4444
3776
14483
14425
1674
515217
20G73-107times1305
2228
2433
2583
2415
6340
5794
6373
6169
12490
1015
8119
61
11536
4199
4199
5018
4472
18830
1595
218334
1770
6
21G73-107times(77371times
BSEC
TCP
-1)
1943
2128
2220
2097
3936
3980
5770
4562
11697
9858
10303
10619
1874
2369
4181
2808
15633
13839
16073
1518
222
G73-107times96AC
-93
2118
2218
2317
2217
4270
5843
6201
5438
10319
9872
1015
910117
2703
4460
4379
3847
14589
15715
16359
15555
23G73-107timesTo
go-II
2237
2365
2733
2445
5736
5175
6822
5911
12098
10808
1299
611967
3984
3670
5372
4342
17835
1598
219818
17878
24G73-107times99HS-18
2025
2081
2322
2143
3558
4229
6612
4800
12350
9685
11516
1118
42196
2782
5009
3329
1590
813914
1812
915983
2577245timesCV
J-2-5-3-1-3
2109
2360
2675
2381
6760
6558
7299
6872
15225
11861
1416
21374
94225
4752
5839
4939
2198
518418
21461
20621
2677245times1305
1919
2100
2197
2072
5700
5142
5347
5397
11687
10565
10737
10996
4318
3956
4244
4173
17386
1570
816085
16393
2777245times(77371times
BSEC
TCP
-1)
1851
1935
2020
1936
2577
2460
2838
2625
6989
4778
4636
5468
1631
1662
1999
1764
9566
7238
7474
8093
2877245times96AC
-93
1729
1780
2133
1881
5541
5058
5748
5449
11952
8174
9526
9884
3646
3587
4071
3768
17493
13232
15274
15333
2977245timesTo
go-II
2300
2508
2658
2489
3067
5431
5963
4820
11737
11021
1216
411641
1598
4053
4770
3474
14805
16452
1812
616461
3077245times99HS-18
1982
2019
2223
2075
3846
3119
4225
3730
7493
6720
6476
6896
3205
2578
2643
2809
11339
9839
1070
110626
12 Advances in Agriculture
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
31CV
J-2-5-3-1-3times1305
2406
2553
3067
2675
4178
4796
6281
5085
13822
11425
12252
12500
2472
3241
4907
3540
18000
16222
18532
17585
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
2142
2173
2238
2184
4522
5235
5640
5132
11673
9658
10248
10526
2614
3766
4178
3519
1619
514893
15888
15659
33CV
J-2-5-3-1-3times
96AC
-93
2350
2477
2600
2476
4676
4280
6090
5015
11975
9832
10868
10891
3097
2931
4445
3491
16651
14111
1695
815907
34CV
J-2-5-3-1-3times
Togo-II
2093
2666
2989
2583
4058
3727
5190
4325
12588
10717
11217
11507
2742
2570
4185
3166
16645
14443
16407
15832
35CV
J-2-5-3-1-3times
99HS-18
2028
2311
2386
2242
3287
2513
5356
3719
10055
9733
1036
410051
2004
1581
4251
2612
13342
12246
1572
01376
9
361305times(77371times
BSEC
TCP
-1)
2005
2173
2210
2129
3569
4692
5140
4467
11652
9826
10041
10507
2005
3665
4179
3283
15221
14518
1518
214974
371305times96AC
-93
1822
2003
2047
1957
5115
4023
4813
4650
9965
7675
7571
8404
3456
2647
3155
3086
15080
11698
12384
13054
381305timesTo
go-II
2545
2583
2644
2591
6885
7283
6795
6987
12265
11410
12583
12086
4500
6120
5350
5323
1915
018693
19378
19074
391305times99HS-18
2070
2408
2593
2357
5219
4332
4800
4784
1092
97677
8968
9192
3924
3332
3840
3699
1614
912009
1376
913976
40(77371times
BSEC
TCP
-1)
times96AC
-93
2056
2115
2157
2109
3158
4463
4717
4113
9166
7540
8100
8268
1880
3542
3347
2923
12324
12003
12817
12381
41(77371times
BSEC
TCP
-1)
timesTo
go-II
1868
2253
2450
2190
4422
6458
7038
5973
1415
312369
1379
713439
2291
4646
5586
4174
18575
18827
20835
19412
42(77371times
BSEC
TCP
-1)
times99HS-18
1995
2026
2209
2077
2720
2909
3543
3057
7392
4692
5023
5702
1766
2187
2147
2033
10112
7601
8566
8760
4396AC
-93timesTo
go-II
1953
2027
2511
2164
5972
4391
7126
5830
15537
1392
714432
14632
3780
2538
5655
3991
21510
18318
21558
20462
4496AC
-93times99HS-18
1952
2021
2242
2072
3465
2758
4496
3573
5959
5186
6094
5746
2707
2154
2409
2423
9424
7944
10590
9319
45To
go-IItimes
99HS-18
1932
2008
2658
2199
5381
4995
5436
5270
10299
8876
9244
9473
3986
3842
3668
3832
15680
13871
14680
1474
3Mean
1982
2146
2301
2143
3963
4123
4836
4307
9498
8087
8663
8749
2510
2914
3513
2979
13460
12210
13499
13056
SE243
198
245
mdash671
501
626
mdash959
750
822
mdash423
349
344
mdash1155
927
1167
mdashCD
087
071
087
mdash239
178
223
mdash342
267
293
mdash15
112
412
2mdash
411
330
416
mdash
Advances in Agriculture 13Ta
ble4Analysis
ofvaria
ncefor
10times10
half-diallelfor
different
charactersin
threee
nviro
nments
(a)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Germination
()
Rateof
emergence
STI()
SSTI
()
Num
bero
fleavesseedling
Seedlin
gheight
(cm)
Freshweight
(g)seedlin
gDry
weight
(g)seedlin
g
E 1
Replicates
2652731
596768
783393
20700
93018674
33764
1033999
002590
Treatm
ents
541097
0255lowastlowast
8181153lowastlowast
30863092lowastlowast
20680900lowastlowast
1100708lowastlowast
2106317lowastlowast
930829527lowastlowast
319767lowastlowast
Parents
95118
163lowastlowast
14289925lowastlowast
59114816lowastlowast
18880254lowastlowast
1084227lowastlowast
1087906lowastlowast
22561719lowastlowast
042162lowastlowast
Hybrid
s44
11423573lowastlowast
3398933lowastlowast
25697199lowastlowast
20633716lowastlowast
472874lowastlowast
1391526lowastlowast
674123518lowastlowast
261839lowastlowast
Parentsv
ersush
ybrid
s1
43693104lowastlowast
163619
916lowastlowast
3896838
3896
2793lowastlowast
28873741lowastlowast
4272
2798lowastlowast
234144289lowastlowast
5367022lowastlowast
Error
108
281184
1356832
1173684
674337
040104
306
462
2015
1619
005535
E 2
Replicates
2583819
3707389lowast
1596635
198738
034925
448213
899529
007177
Treatm
ents
549997443lowastlowast
2825177lowastlowast
35378177lowastlowast
19074306lowastlowast
665284lowastlowast
1764
081lowastlowast
257694570lowastlowast
2177519lowastlowast
Parents
96507784lowastlowast
3718
879lowastlowast
69866289lowastlowast
1798
5404lowastlowast
705379lowastlowast
1408518lowastlowast
10612519lowastlowast
077429lowastlowast
Hybrid
s44
10569731lowastlowast
2127302lowastlowast
2910
2537lowastlowast
19429240lowastlowast
329256lowastlowast
7000
03lowastlowast
186026552lowastlowast
1483428lowastlowast
Parentsv
ersush
ybrid
s1
16223710lowastlowast
25488361lowastlowast
1113
326
13257362lowastlowast
15089673lowastlowast
5178
3569lowastlowast
5634825791lowastlowast
51618348lowastlowast
Error
108
334760
1097039
1049505
635570
06764
116
0576
694613
006206
E 3
Replicates
2894398
4808815lowast
mdashmdash
051076
095989
3768421
030220
Treatm
ents
541261560
0lowastlowast
4842216lowastlowast
mdashmdash
2811077lowastlowast
5947316lowastlowast
3870844
50lowastlowast
3445076lowastlowast
Parents
97135
735lowastlowast
1097
6388lowastlowast
mdashmdash
1468699lowastlowast
1112
764lowastlowast
136081650lowastlowast
1307836lowastlowast
Hybrid
s44
13217740lowastlowast
1379360
mdashmdash
15540
71lowastlowast
4581632lowastlowast
252084767lowastlowast
2378032lowastlowast
Parentsv
ersush
ybrid
s1
35440
180lowastlowast
102000335lowastlowast
mdashmdash
70200767lowastlowast
109548362lowastlowast
8586095705lowastlowast
69630188lowastlowast
Error
108
782907
1085444
mdashmdash
139771
291325
3019
169
02400
4(b)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Panicle
emergence
Effectiv
etillersplant
Planth
eight
Earlength
Earw
eightp
lant
Dry
fodd
eryieldplant
Grain
yieldplant
Totalbiological
yieldplant
E 1
Replicates
231860
61lowastlowast
416348lowastlowast
1672
9472lowast
1706
411077192
9634539
392777
115544
59Treatm
ents
544152
368lowastlowast
085657lowastlowast
326706089lowastlowast
1602572lowastlowast
6177
0406lowastlowast
304113
204lowastlowast
34285030lowastlowast
575617
582lowastlowast
Parents
94511481lowastlowast
018538lowastlowast
140807476lowastlowast
2240093lowastlowast
11653
959lowastlowast
27014155lowastlowast
3858650lowastlowast
61321919lowastlowast
Hybrid
s44
3201347lowastlowast
100797lowastlowast
110395657lowastlowast
1010
497lowastlowast
40296671lowastlowast
171271411lowastlowast
2413
6880lowastlowast
302999650lowastlowast
Parentsv
ersush
ybrid
s1
4276
5286lowastlowast
02360
9lowast11517452
616lowastlowast
2191
6167lowastlowast
1457662749lowastlowast
8643043524lowastlowast
7546
41053lowastlowast
17199467546lowastlowast
Error
108
468159
004907
3692099
224610
17114
303498126
682350
5075268
E 2
Replicates
2656364
002556
61575565lowastlowast
828842lowastlowast
589094
2268506
260877
1507116
Treatm
ents
542999618lowastlowast
069822lowastlowast
268998723lowastlowast
2436886lowastlowast
60592784lowastlowast
247259877lowastlowast
4192
0073lowastlowast
513483489lowastlowast
Parents
926300
00lowastlowast
048079lowastlowast
165555
405lowastlowast
3871874lowastlowast
1412
2393lowastlowast
19543682lowastlowast
3132
426lowastlowast
57230706lowastlowast
Hybrid
s44
2533
165lowastlowast
07344
9lowastlowast
9616
2857lowastlowast
1463359lowastlowast
35495366lowastlowast
154759288lowastlowast
26262465lowastlowast
293492304lowastlowast
Parentsv
ersush
ybrid
s1
26850135lowastlowast
105929lowastlowast
880476
6697lowastlowast
32357161lowastlowast
1583112746lowastlowast
6366731560lowastlowast
107994
3646lowastlowast
14299370699lowastlowast
Error
108
507598
005829
5234122
149406
955133
2142411
462311
3273886
E 3
Replicates
2029697
021161
9459847
143746
985141
46800
6492
6334
3345160
Treatm
ents
548045499lowastlowast
094511lowastlowast
192420108lowastlowast
3279225lowastlowast
72049211lowastlowast
298953
021lowastlowast
55061308lowastlowast
651732994lowastlowast
Parents
98010
741lowastlowast
094166lowastlowast
116037343lowastlowast
3702185lowastlowast
16305190lowastlowast
2323644
0lowastlowast
4553
893lowastlowast
71484982lowastlowast
Hybrid
s44
6254377lowastlowast
095514lowastlowast
7378
1513lowastlowast
2188348lowastlowast
31813193lowastlowast
190263916lowastlowast
31572179lowastlowast
368689934lowastlowast
Parentsv
ersush
ybrid
s1
8716
7710lowastlowast
053496lowast
609996
3199lowastlowast
47471212lowastlowast
2344
130203lowastlowast
756272
2861lowastlowast
154314
9707lowastlowast
18327859
719lowastlowast
Error
108
696981
007883
5578505
228608
1491014
2574
223
449588
5187705
lowast
Sign
ificant
at119875=005
lowastlowastSign
ificant
at119875=001
14 Advances in Agriculture
Table 5 Mean performance of the genotypes for membrane thermostability in two environments
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594
Advances in Agriculture 15
Table 5 Continued
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434
Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash
lowastValues are transformed values
0
6
12
18
24
30
36
42
48
54
minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity
G73-10796AC-93CVJ-2-5-3-1-3
Togo-II99HS-18
H77833-2H7729-2772451305
H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305
1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305
H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305
77245 times 1305
77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305
77371 times BSECT CP-1
H77833-2 times G73-107
Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits
moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling
survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The
16 Advances in Agriculture
Table6Po
oled
correla
tioncoeffi
cientamon
gvario
uscharactersin
threee
nviro
nments
Characters
Germination
Rateof
emergence
STI
SSTI
Leaves
seedlin
gSeedlin
gheight
Fresh
weight
Dry
weight
MTS
Panicle
emergence
Effectiv
etillersplant
Plant
height
Earlength
Ear
weightp
lant
Dry
fodd
eryield
plant
Grain
yield
plant
Total
biological
yield
plant
Germination
1000
0357lowastlowast
0353lowastlowast
0590lowastlowast
0230
0373lowastlowast
0293lowast
0260
0276lowastminus0135minus0478lowastlowast
0326lowast
minus0069minus0104
minus0190
minus0145
minus0167
Rateof
emergence
1000
0233
0351lowastlowast
0605lowastlowast
0585lowastlowast
0551lowastlowast
0524lowastlowast
0139minus0593lowastlowast
0032
0517lowastlowast
0217
0388lowastlowast
0361lowastlowast
0348lowastlowast
0377lowastlowast
STI
1000
0837lowastlowast
0241
0129
0081
004
80890lowastlowast
minus0215
minus0211
0030minus0007minus0043
minus0101
minus0111
minus0084
SSTI
1000
0233
0264
0183
0140
0745lowastlowast
minus0247minus0268lowast
0184minus0017minus006
6minus0129
minus0144
minus0112
Num
bero
fleaves
1000
0683lowastlowast
0633lowastlowast
060
4lowastlowast
0176minus0474lowastlowast
0109
0576lowastlowast
0496lowastlowast
064
1lowastlowast
0607lowastlowast
0630lowastlowast
0630lowastlowast
Seedlin
gheight
1000
0703lowastlowast
0663lowastlowast
0031minus0493lowastlowast
minus0115
0735lowastlowast
0360lowastlowast
0526lowastlowast
0520lowastlowast
0515lowastlowast
0532lowastlowast
Freshweight
1000
0957lowastlowast
minus0016
0425lowastlowast
minus0082
0661lowastlowast
0351lowastlowast
0581lowastlowast
0496lowastlowast
0576lowastlowast
0533lowastlowast
Dry
weight
1000minus0033minus0428lowastlowast
minus0016
0634lowastlowast
0354lowastlowast
0620lowastlowast
0509lowastlowast
0614lowastlowast
0554lowastlowast
MTS
1000minus040
minus0177minus0037
0015
minus0074
minus0138
minus0131
minus0120
Panicle
emergence
1000
minus0150minus0269lowast
0022minus0329lowast
minus0337lowast
minus0280lowast
minus0341lowast
Effectiv
etillersplant
1000
minus0012
0170
0366lowastlowast
040
5lowastlowast
0323lowast
0401lowastlowast
Planth
eight
1000
0650lowastlowast
0603lowastlowast
0596lowastlowast
0619lowastlowast
0610lowastlowast
Earlength
1000
0608lowastlowast
0671lowastlowast
064
8lowastlowast
066
4lowastlowast
Earw
eightp
lant
1000
0910lowastlowast
0984lowastlowast
0957lowastlowast
Dry
fodd
eryield
plant
1000
0907lowastlowast
0991lowastlowast
Grain
yield
plant
1000
0950lowastlowast
Totalbiological
yield
plant
1000
lowastSign
ificant
at119875=005lowastlowastSign
ificant
at119875=001
Advances in Agriculture 17
faster germination and early vigour in seedling could act asphysical indicators for heat tolerance
Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits
Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics
Conflict of Interests
The authors declare that there is no conflict of interestsregarding the publication of this paper
References
[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981
[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993
[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006
[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993
[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013
[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007
[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965
[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011
[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001
[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009
[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962
[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967
[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963
[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001
Submit your manuscripts athttpwwwhindawicom
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Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
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Applied ampEnvironmentalSoil Science
Volume 2014
AgricultureAdvances in
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PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
BiodiversityInternational Journal of
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ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
GenomicsInternational Journal of
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Plant GenomicsInternational Journal of
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Biotechnology Research International
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Advances in Agriculture 3
0
10
20
30
40
50
60
70
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15DAS
E1
E2
E3
Tem
pera
ture
(∘C)
(a)
05
101520253035404550
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15DAS
E1
E2
E3
Tem
pera
ture
(∘C)
(b)
0
10
20
30
40
50
60
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16DAS
E1
E2
E3
Tem
pera
ture
(∘C)
(c)
02468
101214161820
1 3 5 7 9 11 13 15
Moi
sture
()
DAS
E1
E2
E3
Permanent wilting point
(d)
Figure 1 (a) Soil surface temperature (b) air temperature (c) temperature 5 cm above the soil surface and (d) soil moisture status in differentenvironments
30 cm apart with 10 cm plant to plant spacingThe seeds weresown manually
The soil of experimental field was sandy loam in textureThe water holding capacity field capacity and permanentwilting point of the experimental field were 40 17 and 6percent respectively [6] Irrigation was withheld during thecourse of experiment however drought stress-free condi-tions were ensured through monitoring at regular interval(on alternate days) by measuring the moisture status of thesoil by gravimetric method The soil samples of about 50 gwere taken up to 20 cmdepth from the experimental plotThemoisture content on dry weight basis may be calculated [7]using the following formula
Soil moisture ()
=
(Weight of wet soil minusWeight of dry soil)Weight of dry soil
times 100
(1)
The heat stress period was terminated by irrigating theexperimental field before reaching the soil moisture level atpermanent wilting point A condition of no drought wasmaintained in order to determine the exclusive effect of tem-perature at seedling stageThis was maintained by measuringthe moisture status of the soil by gravimetric method onalternate days The soil surface temperature was measuredby a soil thermometer at soil surface temperature at 5 cmabove the soil surface and air temperature at 1m above thesoil surface were recorded between 200 pm and 230 pmdaily (Figure 1)
Emphasis of observation is centred on germination sur-vival and mortality of the seedlings
23 Plant CharacteristicsScreening Techniques The empha-sis was given to the observations of germination survival andmortality of the seedlingsThe seedlings were inspected everyday in the morning The seedlings which had died due to
4 Advances in Agriculture
heat stress were noted in each genotype on daily basis Obser-vations continued till the surviving seedlingswere established(15 DAE) and there was no further mortality Moreover thesoil moisture was depleting near to the permanent wiltingpoint and drought stress could also come into play along withheat stress to further continue the experiment Since experi-ment proceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedlingsurvival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of heat stress at seedling stage was observed on latergrowth stages
231 Thermotolerance Characteristics For heat tolerancecharacteristics 3 indices namely seedling thermotoleranceindex (STI) [4] seed to seedling thermotolerance index(SSTI) [8] and membrane thermostability (MTS) wereapplied to evaluate the hybrids and parents STI was cal-culated as the ratio of number of seedlings survived to thenumber of seedling emergences expressed in percentageSeed to seedling thermotolerance index (SSTI) was calculatedas the ratio of seedling survival to the number of seedlingsexpected to emerge expressed in percentage [8] The SSTIis an extension of STI by taking expected germination intoaccount It was necessary to correct the effect of under soilmortality (USM) The loss of germination due to heat (ieunder soil mortality) can be measured only after a standardgermination test Therefore the germination of seed fromthe same lot under monsoon environment (main pearl milletgrowing season where no under soil mortality occurreddue to heat stress) was taken as expected germination forcalculation of SSTI
232 Membrane Thermostability These genotypes were alsoscreened through laboratory technique (membrane ther-mostability) [9 10] The young leaves of 21-day-old seedlingsof each genotype in three replication plots were used formembrane thermostability test A middle portion of 10 cmfrom each leaf was taken and the midrib was removed andtwo equal halves of the 10 cm each were made Each halfwas taken to provide replications Each leaf portion was cutinto pieces and thoroughly washed and rinsed with distilledwater two times to remove the electrolytes adhering to theplant tissue as well as electrolyte released from the cutting ofplant tissue and put them into test tubes After final rinsingtubes were drained maintaining sufficient water to preventdesiccation of plant material during heat treatment Thesetest tubes were covered with aluminium foil and incubated inwater bath at 55∘C for 15 minutes for giving heat treatmentAfter the heat treatment period 10mL of distilled waterwas added to the test tubes Then all the test tubes wereheld at 10∘C overnight to allow diffusion of electrolytes fromplant material Test tubes were brought out of refrigeratorand shaken well to mix the contents An initial electricalconductivity (EC) of both replications of each treatment (119879
1)
was determined with electrical conductivity meter Thenthese test tubes were autoclaved at 15 psi for 2 minutes forcomplete electrolyte leakage from the leaf samples These testtubes were then cooled at room temperature and contentsmixed and final EC was taken for all the treatments (119879
2)
Membrane thermostability (MTS) was calculated accordingto Ibrahim and Quick [9] and Yadav et al [10] as follows
Membrane Thermostability (MTS) = (1 minus1198791
1198792
) times 100
(2)
where 1198791is conductivity reading after heat treatment and 119879
2
is conductivity reading after autoclavingCompletely randomized design (CRD) for membrane
thermostabilitywas conducted to detect variations among thegenotypes
233 Morphological Characteristics The seedling growthcharacteristics namely germination () emergence rate(ER) number of leavesseedling (two-week stage) seedlingheight (two-week stage) seedling fresh weight (g) (four-week stage) and seedling dry weight (g) (four-week stage)were recorded The data for seedling height and number ofleaves per seedling was recorded on 10 random seedlings perplot Emergence rate (an index for speed of germination)can be used as a tool in pearl millet breeding programs forevaluation of seedling vigour Speed of germination is oneof the oldest concepts of seedling vigour The emergencerate (ER) was calculated by dividing the number of normalseedlings obtained at each counting by the number of daysseeds had been in the seed bed (DAS)The values obtained ateach count were then summed at the end of the germinationtest to obtain the emergence rate [11]
Emergence Rate (ER) =Seedling count
Days to first count+ sdot sdot sdot
+
Seedling countDays to final count
(3)
After termination of the heat stress on the seedlings byirrigating the field the experiment was continued to studythe effect of seedling heat stress on maturity traits on thesurvived plants The maturity traits observed were panicleemergence effective tillersplant plant height ear length earweightplant dry fodder yieldplant grain yieldplant andtotal biological yieldplant
24 Statistical Analysis Analysis of variance for randomizedblock design (RBD) for field traits that is germinationemergence rate number of leavesseedling seedling heightseedling fresh weight and seedling dry weight and com-pletely randomized design (CRD) for membrane thermosta-bility test was conducted under each environment on meanbasis [12] Analyses were done using the software OPSTATdeveloped at CCS HAU Hisar Correlations among the vari-ous characters studied were calculated to establish the natureand magnitude of associations among the traits [8 13] with
Advances in Agriculture 5
the software SPSS (SPSS Inc) Multivariate clustering wasdone based on paired group cluster method using similaritycoefficient under Past 140 software [14] Multivariate analysisbased on the Euclidean distancematrix was estimated amonggenotypes to distinguish genotypes from each other Thegrouping of genotypes was generated using paired groupcluster method
3 Results and Discussion
The excessive heat (soil temperature up to 630∘C in E2)
about 18∘C higher than the normal atmospheric temperature(Figures 1(a) and 1(b)) was responsible for mortality ofseedlings Many seedlings were found desiccated at the pointof contact besides the desiccation at the tipsThe temperaturearound the tip level (5 cm above the soil surface) also reachedup to 50∘C (Figure 1(c)) It could therefore be concluded thatthe seedlings had to survive in a really high temperature Themoisture level remained above the permanent wilting point(Figure 1(d)) therefore drought effect was not in the playThe heat tolerance was measured as STI (Table 3) throughthe field screening technique of Peacock et al [4] Anotherindex known as SSTI was also calculated as an improvementand extensionThe analysis of variance (RBD) for 8 charactersin 3 environments showed significant genotypic differencesNo variability was observed for STI and SSTI in E
3only In
this environment no seedling mortality was observed andthe germination was taken as standard The values for STIand SSTI were therefore equal and the highest for all thegenotypes in this environment and hence no variation wasobserved
The hybrids were evaluated on the basis of mean perfor-mance for various characters in each environment (Table 3)On the mean basis STI value ranged from 5332 to 8224with an average of 7072 The hybrid H7729-2 times CVJ-2-5-3-1-3 was the best scorer for heat tolerance followed byH77833-2 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II The hybrid H77833-2 times 1305 was found susceptible toheat stress Seed to seedling thermotolerance index (SSTI)which is expected to be more rigorous index deviated thevalues from STI according to variation in germination undernormal environment (E
3) as the SSTI also coversincludes
the under surface mortality (USM) during germinationThe reductions in values were not strictly proportional tothe STI The reduction is dependent on thermosensitivityof germination We also came across such hybrids whichshowed poor germination and high STI SSTI values rangedfrom 4605 to 7287 with an average of 6090 The genotypeH77833-2 times 96AC-93 had the highest worth for SSTI onmean basis followed byG73-107times (77371timesBSECTCP-1) andH77833-2 times (77371 times BSECT CP-1) (Table 3) The analysisof variance for ten different characters in three differentenvironments (two stressed and one normal) was carried outand the results showed significant genotypic differences forall the characters (Table 4)
A caution is needed for evaluating such genotypes underSTI The SSTI gave a highly reduced value putting thesecrosses in the susceptible list It may be concluded therefore
that SSTI is more dependable and desirable tool for screeningand evaluating the genotypes for heat tolerance The germi-nation was lower in E
2than in E
1 This period has the highest
range of temperature with a high average Genotype-wise thehighest germination (6209) was recorded for the genotypeH7729-2 times (77371 times BSECT CP-1) on mean basis whichgives a preliminary indication of resistance to heat while thegenotype 1times 6 had the lowest germination (3533)The hybridH77833-2 times G73-107 was adjudged as the fastest emergenceof seedlingsThe genotype (77371timesBSECTCP-1)times 96AC-93had the highest fresh weightseedlingThe genotype H7729-2 times Togo-II had the highest seedling dry weight E
1had lower
dry weightseedling than E2indicating the more heat stress
during the E1
31 Membrane Thermostability Membrane thermostability(MTS) has long been recognised as a parameter of heattolerance All the hybrids were put to membrane thermosta-bility test in the laboratory under standard protocol Acompletely randomised design (CRD) analysis was carriedout for membrane thermostability (MTS) The mean sum ofsquares due to genotypes was highly significant indicatingthat enough genetic variability was present for membranethermostability (MTS) in each environment which justifiedfurther analysis of the data The range of values lay between5073 and 7742 Among the parents H77833-2 G73-107CVJ-2-5-3-1-3 77371 times BSECT CP-1 96AC-93 Togo-II and99HS-18 were verified as resistant genotypes and H7729-2 77245 and 1305 genotypes were found susceptible asmentioned in Table 1 (on the basis of previous experiment)Among all the hybrids H7729-2 times CVJ-2-5-3-1-3 CVJ-2-5-3-1-3 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II werethree highly tolerant genotypes on the basis of membranethermostability (Table 5)
32 Correlations The pooled correlations among the charac-ters were calculated (Table 6) around heat tolerance indices(STI SSTI and membrane thermostability) with a view tofind whether there was any association of these characterswith seedling as well as maturity traits It was observedthat STI and SSTI recorded the highest significant cor-relation among themselves as they are related indices forheat tolerance Membrane thermostability also showed highsignificant correlation with STI as well as SSTI STI hada significant positive association with germination of seedonly among seedling and maturity traits studied SSTI inaddition was also positively correlated with germination andemergence rate and negatively associated with number ofeffective tillersplantMembrane thermostability also showedsignificant positive correlation with seed germination amongseedling and maturity traits studied While evaluating thediallel progenies it was also seen that the heat toleranceindices STI SSTI and membrane thermostability were notshowing any perceptible correlation with the rest of thedevelopmental traits (seedling andmaturity traits)The stressperiod was terminated by irrigating the field experiment after15 days fromdate of sowingwhen the seedlings get establishedand further mortality stopped due to heat Moreover the soil
6 Advances in Agriculture
Table3Perfo
rmance
ofthep
arentsandthec
rosses
ford
ifferentcharacters
(a)
Sr
No
Genotypes
Germination(
)Ra
teof
emergencelowast
STIlowast
()
SSTIlowast(
)Num
bero
fleavesseedlin
gE 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
3941
3737
6164
4614
4037
4109
4279
4142
7830
7387
9000
8072
4498
4133
9000
5877
661
502
800
654
2H7729-2
5513
4726
6092
5443
2815
4980
3258
3684
4835
4495
9000
6110
4441
3602
9000
5681
363
294
427
361
3G73-107
5037
4826
5918
5260
4534
4132
4481
4382
6187
5413
9000
6866
5213
4460
9000
6224
878
710
935
841
477245
4747
4624
5565
4978
3625
4604
4047
4092
4385
3580
9000
5655
3803
3040
9000
5281
377
292
437
369
5CV
J-2-5-3-1-3
4824
4832
6897
5518
5044
4907
4960
4970
7193
6813
9000
7669
4959
4785
9000
6248
802
580
923
768
61305
4326
3891
5404
4540
3376
5056
3573
4002
3903
3281
9000
5395
3211
2452
9000
4888
350
284
414
349
777371timesBS
ECTCP
-14747
4502
6320
5190
4934
5075
5065
5025
6952
6706
9000
7552
5056
4671
9000
6242
621
542
700
621
896AC
-93
4651
3818
5722
4730
4312
4915
4753
4660
6660
6001
9000
7220
5212
3920
9000
6044
701
590
899
730
9To
go-II
4613
3937
5276
4609
4245
4737
4797
4593
7198
6861
9000
7686
5980
4767
9000
6582
681
629
853
721
1099HS-18
4575
3823
6247
4882
4317
4659
4746
4574
7594
7319
9000
7971
5154
4172
9000
6109
745
554
915
738
1H77833-2timesH7729-2
5787
5295
6733
5938
4964
5015
4989
4989
6803
6504
9000
7436
5808
5134
9000
6647
711
585
780
692
2H77833-2timesG73-107
5472
4417
6405
5431
5448
5422
5239
5370
7231
6769
9000
7667
5980
4556
9000
6512
1101
705
1340
1049
3H77833-2times77245
4076
3707
5504
4429
5217
5446
5238
5300
6627
6224
9000
7284
4690
4037
9000
5909
740
525
965
744
4H77833-2timesCV
J-2-5-3-1-3
4843
4300
5856
5000
4862
4956
4913
4911
7061
6519
9000
7527
5575
4632
9000
6403
998
885
1258
1047
5H77833-2times1305
3296
2780
4521
3533
5076
4907
5238
5074
3732
3265
9000
5332
2765
2050
9000
4605
1192
934
1600
1242
6H77833-2times(77371times
BSEC
TCP
-1)5636
5539
6258
5811
4489
5219
5015
4908
6981
6482
9000
7488
6146
5680
9000
6942
898
726
1525
1050
7H77833-2times96AC
-93
5720
5515
6335
5857
4800
5186
5075
5021
7833
7786
9000
8206
6603
6256
9000
7287
1107
873
1193
1058
8H77833-2timesTo
go-II
4862
3453
5709
4675
5036
4829
4911
4925
6190
5500
9000
6897
5214
3339
9000
5851
979
670
1107
919
9H77833-2times99HS-18
4575
4418
5447
4813
5041
5074
5006
5040
5800
5073
9000
6625
4846
4133
9000
5993
978
733
1135
949
10H7729-2timesG73-107
5615
4962
6273
5617
5023
5535
5165
5241
6143
5501
9000
6881
5540
4441
9000
6327
1000
720
1363
1028
11H7729-2times77245
5472
5164
6281
5639
4745
5031
5028
4935
5572
5175
9000
6582
4901
4345
9000
6082
880
620
897
799
12H7729-2timesCV
J-2-5-3-1-3
5494
5124
6536
5718
4804
5316
4957
5026
7596
8076
9000
8224
6093
5680
9000
6924
981
932
1451
1121
13H7729-2times1305
3804
3826
5511
4380
3596
4724
4387
4236
6362
7075
9000
7479
4230
4517
9000
5916
926
725
1497
1049
14H7729-2times(77371times
BSEC
TCP
-1)
6369
4847
7412
6209
4847
5351
5246
5148
6377
6031
9000
7136
5683
4230
9000
6304
977
671
1380
1009
15H7729-2times96AC
-93
5314
4657
6702
5558
4514
5295
5102
4971
5612
4856
9000
6489
4613
3603
9000
5739
846
679
1397
974
16H7729-2timesTo
go-II
5827
5594
6651
6024
5072
5279
5271
5207
6563
5371
9000
6978
5763
4652
9000
6472
873
719
1040
877
17H7729-2times99HS-18
5741
4638
7918
6099
4847
5024
5021
4964
6179
5603
9000
6927
4882
3725
9000
5869
1012
730
1600
1114
18G73-107times77245
5115
4646
6103
5288
4977
5242
5450
5223
7006
6063
9000
7356
5685
4594
9000
6426
1021
790
1292
1034
19G73-107timesCV
J-2-5-3-1-3
5134
4894
6427
5485
4901
5317
5181
5133
6637
6124
9000
7254
5253
4710
9000
6321
1039
863
1254
1052
20G73-107times1305
4881
4561
7597
5680
4717
4791
4775
4761
6704
6380
9000
7361
4556
4115
9000
5890
1140
882
1657
1226
21G73-107times(77371times
BSEC
TCP
-1)
5636
5519
6283
5812
4986
5219
5203
5136
7990
6203
9000
7731
6620
5436
9000
7019
977
703
1395
1025
22G73-107times96AC
-93
5740
4510
6415
5555
5129
4982
5195
5102
6978
5556
9000
7178
6156
4019
9000
6392
1077
860
1264
1067
23G73-107timesTo
go-II
4460
4357
6044
4953
4396
5138
4951
4829
5279
4835
9000
6371
4018
3603
9000
5540
906
881
1189
992
24G73-107times99HS-18
5473
4356
6374
5401
5102
4955
5055
5037
6460
5197
9000
6886
5534
3704
9000
6079
1171
766
1635
1191
2577245timesCV
J-2-5-3-1-3
4766
4666
5921
5117
5170
4918
4900
4996
5928
5859
9000
6929
4785
4613
9000
6133
984
920
1194
1033
2677245times1305
4575
4060
6052
4895
5128
5297
5203
5210
5102
4990
9000
6364
3979
3464
9000
5481
1279
823
1550
1217
2777245times(77371times
BSEC
TCP
-1)
5017
4929
6022
5322
5108
5473
5274
5285
5308
5044
9000
6451
4498
4191
9000
5896
833
588
939
787
Advances in Agriculture 7
(a)Con
tinued
Sr
No
Genotypes
Germination(
)Ra
teof
emergencelowast
STIlowast
()
SSTIlowast(
)Num
bero
fleavesseedlin
gE 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
2877245times96AC
-93
5472
4705
622154
66
5135
5197
5006
5113
5247
4843
9000
6363
4708
3784
9000
5831
795
662
1058
838
2977245timesTo
go-II
5252
4492
5889
5211
5188
4861
5074
5041
4885
4263
9000
6049
4421
3379
9000
5600
977
751
1243
990
3077245times99HS-18
5740
4099
6872
5570
5249
4898
5258
5135
5628
4616
9000
6415
4863
3014
9000
5626
1045
863
1184
1031
31CV
J-2-5-3-1-3times1305
4364
3944
5700
4669
4974
4517
4650
4714
6097
5579
9000
6892
4594
3842
9000
5812
936
848
1403
1063
32CV
J-2-5-3-1-3times(77371times
BSEC
TCP
-1)4345
3808
6774
4976
5272
4996
5035
5101
6650
5766
9000
7139
4288
3381
9000
5556
800
712
1013
841
33CV
J-2-5-3-1-3times96AC
-93
4862
4197
5743
4934
4884
4980
5058
4974
7477
6760
9000
7745
5957
4709
9000
6555
933
738
1478
1050
34CV
J-2-5-3-1-3timesTo
go-II
4670
4343
6233
5082
4399
4813
4631
4614
5806
6614
9000
7140
4422
4479
9000
5967
963
855
1180
999
35CV
J-2-5-3-1-3times99HS-18
5493
5080
7273
5949
5276
4984
5162
5141
7322
6590
9000
7637
5534
4766
9000
6433
998
807
1338
1047
361305times(77371times
BSEC
TCP
-1)
4613
4539
5717
4956
4986
4955
5080
5007
5137
4870
9000
6336
4210
3936
9000
5715
863
630
913
802
371305times96AC
-93
5473
5177
6830
5827
5224
4779
4919
4974
7117
6965
9000
7694
5640
5220
9000
6620
875
694
1476
1015
381305timesTo
go-II
4230
3420
5262
4304
4309
456144
45
4438
5781
4373
9000
6385
4575
2887
9000
5487
637
566
794
666
391305times99HS-18
5433
4507
6947
5629
5225
4753
4991
4989
7287
6968
9000
7752
5576
4479
9000
6352
1115
818
1410
1114
40(77371times
BSEC
TCP
-1)times
96AC
-93
5595
4429
7461
5828
5216
4955
5121
5097
7087
6368
9000
7485
5432
4038
9000
6157
955
753
1462
1057
41(77371times
BSEC
TCP
-1)times
Togo-II
5252
4728
6106
5362
5492
4808
5132
5144
8025
7505
9000
8177
6310
5413
9000
6908
859
693
990
848
42(77371times
BSEC
TCP
-1)times
99HS-18
5595
4298
6295
5396
4729
4887
5031
4883
7366
6945
9000
7770
6312
4556
9000
6623
903
622
1125
883
4396AC
-93timesTo
go-II
5017
3977
6486
5160
5019
5195
5237
5150
5922
5486
9000
6803
4689
3484
9000
5724
1007
728
1167
967
4496AC
-93times99HS-18
5595
4812
6924
5777
5036
5385
5205
5209
7411
5378
9000
7263
5873
3979
9000
6284
1013
652
1334
1000
45To
go-IItimes
99HS-18
4670
4497
7293
5487
4718
4319
4796
4611
5688
5431
9000
6706
3980
3665
9000
5548
939
653
1462
1018
Mean
5043
4482
6271
5265
4792
498149
23
4899
6377
584190
00
7072
5079
4190
9000
6090
898
701
1168
922
SE097
106
161
mdash213
191
190
mdash19
818
7mdash
mdash14
914
6mdash
mdash036
047
068
mdashCD
272
296
453
mdash597
537
534
mdash555
525
mdashmdash
421
409
mdashmdash
103
133
192
mdashlowastTransfo
rmed
data
(b)
Sr
No
Genotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
908
785
2245
1313
859
1537
1924
1440
154
143
160
152
6267
4800
3833
4967
212
227
249
229
15644
15458
13267
1479
02
H7729-2
1125
1175
1789
1363
317
2400
2811
1843
093
191
252
179
6700
5333
5100
5711
185
210
234
210
18850
18565
15389
17601
3G73-107
1166
1475
1966
1536
943
1744
4945
2544
143
129
366
213
6700
5433
5300
5811
218
219
238
225
15009
1496
513503
14492
477245
1438
1373
2085
1632
607
1863
5957
2809
117
180
509
269
6100
4800
5100
5333
232
221
253
235
14036
13802
11742
1319
35
CVJ-2-5-3-1-3
1214
1437
1807
1486
1163
1610
4166
2313
171
141
407
240
6300
4933
4600
5278
239
227
270
245
1994
317261
1497
017391
61305
858
1137
1737
1244
540
967
4861
2123
125
088
417
210
6533
5033
4800
5456
244
213
257
238
15527
15405
12274
14402
8 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
777371timesBS
ECT
CP-1
1377
1487
1892
1585
928
2660
3330
2306
150
258
301
236
5733
4533
4700
4989
242
220
293
252
1513
814550
1315
014279
896AC
-93
1303
1259
1852
1471
1056
1445
5972
2825
185
147
586
306
5500
4833
3833
4722
256
304
318
293
15577
14032
10000
13203
9To
go-II
1056
1220
2272
1516
924
1466
9113
3834
146
129
883
386
6167
4533
4600
5100
185
149
124
153
14578
12073
10033
12228
1099HS-18
1277
1075
1808
1387
1241
2833
7070
3715
228
226
615
356
6100
5000
4167
5089
243
173
324
247
12627
10355
1017
311052
1H77833-2times
H7729-2
1432
1498
2233
1721
1350
4405
8373
4709
159
499
782
480
5767
4433
3800
4667309
290
248
282
21010
18071
15847
18309
2H77833-2times
G73-107
1675
1635
2205
1838
1236
3211
5789
3412
134
313
547
331
5833
4767
3833
4811
262
295
236
265
21253
20449
15808
1917
0
3H77833-2times
77245
1577
1522
2263
1787
1058
6933
8719
5570
255
553
782
530
5267
4633
4600
4833
273
201
265
246
2074
119511
15208
18487
4H77833-2times
CVJ-2-5-3-1-3
1791
1757
2165
1904
20218030
5276
5109
354
779
477
537
5533
4233
3767
4511
165
330
351
282
21827
20072
17110
19670
5H77833-2times1305
1700
1842
28412128
1427
6228
9134
5596
570
579
813
654
5800
4267
3333
4467340
426
451
406
19816
19444
16037
18432
6H77833-2times
(77371times
BSEC
TCP
-1)
1453
1515
3120
2029
789
465510428
5291
211
408
858
492
5233
4167
3267
4222
203
283
267
251
20677
1913
816470
1876
2
7H77833-2times
96AC
-93
1835
1783
2427
2015
2532
6649
1610
684
29
350
650
1473
824
5467
4233
3700
4467243
239
256
246
22278
20434
15735
19482
8H77833-2times
Togo-II
1564
15632500
1876
1882
4772
11660
6105
296
469
972
579
5667
4533
440048
67
169
260
211
213
2412
82375
619389
22424
9H77833-2times
99HS-18
159318002049
1814
1571
480214024
6799
203
544
1547
765
6033
460040
0048
78
261
225
256
247
23466
21081
1793
320
827
10H7729-2times
G73-107
14951568
2075
1713
959
5157
8517
4877
218
382
666
422
6133
5067
4833
5344
300
273
239
271
23651
2191
717083
20884
11H7729-2times77245
1557
1537
2613
1902
1851
5756
5729
4445366
562
524
484
5633
4800
4633
5022
133
233
297
221
1979
219914
15250
18319
12H7729-2times
CVJ-2-5-3-1-3
18442033
28932257
1651
806114885
8199
264
597
1054
638
6333
4600
4233
5056
170
211
217
199
24629
23339
1970
022556
13H7729-2times1305
1325
1677309920
34
1756
724314048
7682
318
635
1232
728
630046
67
3633
4867298
261
361
307
21666
19371
17422
19486
14H7729-2times(77371
timesBS
ECTCP
-1)
1958
1887
3105
2317
1118
4675
14411
6734
242
457
1318
672
5900
4500
3800
4733
164
209
240
204
23661
1995
419250
20955
15H7729-2times
96AC
-93
1512
1717
2779
2002266844
08
11393
6156
356
500
1211
689
5633
4533
4200
4789
221
195
282
232
2194
921635
17213
20266
Advances in Agriculture 9
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
16H7729-2times
Togo-II
1519
1738
2159
1805
2437
9867
1616
294
89
361
961
1527
950
6167
4700
4200
5022
234
272
230
246
26338
2414
520553
23678
17H7729-2times
99HS-18
1938
1787
3151
2292
1574
6218
11318
6370
290
606
956
617
6067
4767
406749
67
172
210
199
194
24689
21854
1778
921444
18G73-107times77245
1856
1813
2210
1960
2435
8550
7828
6271338
633
612
528
5500
4633
440048
44
199
255
307
254
1993
619277
16095
18436
19G73-107times
CVJ-2-5-3-1-3
1517
1769
2407
1897
1556
7728
9136
6140
239
876
917
677
5900
4300
3400
4533
233
266
315
272
23418
22333
19317
21689
20G73-107times1305
1217
1507
2832
1852
1777
5189
1114
960
38
289
588
1197
691
6733
5000
4233
5322
286
210
312
269
2078
81992
817067
19261
21G73-107times(77371
timesBS
ECTCP
-1)
18551946
2730
2177
1801
3482
10415
5233
239
277
753
423
5500
4467
3433
4467243
197
273
238
23817
20263
17425
20502
22G73-107times
96AC
-93
1718
1840
28612140
1345
9333
14669
8449
297
845
1278
807
5600
5033
4633
5089
270
243
300
271
28537
21461
16875
22291
23G73-107timesTo
go-II
168018083087
2192
1112
4678
9319
5036
186
473
985
548
6033
4767
4200
5000
210
209
231
217
24864
24609
21625
23699
24G73-107times99HS-18
1398
1675
3370
2148
2753
8672
11918
7781
362
691
981
678
566746
00
3500
4589
170
247
270
229
24677
20567
17855
21033
2577245times
CVJ-2-5-3-1-3
1500
1728
2203
1810
2282
4679
11492
6151
328
424
1116
623
6000
4333
420048
44300
231
370
300
21257
20497
1772
519826
2677245times1305
143815772080
1698
949
10439
12053
7814
121
915
992
676
5767
4800
3500
4689
168246
316
244
1978
91779
015517
17699
2777245times(77371times
BSEC
TCP
-1)
150516972846
2016
3222
8518
12621
8120
469
827
1141
812
5400
4367
3833
4533
196
225
242
221
2092
819485
1719
219202
2877245times96AC
-93
1653
1797
2117
1856
1934
6056
11543
6511
352
563
911
608
5333
4267
3833
4478
186
169
198
184
22003
19054
1676
719275
2977245timesTo
go-II
1723
1718
3163
2201
1749
8250
15443
8481229
806
1412
816
5967
4700
4067
4911
174
245
298
239
22075
23503
19875
21818
3077245times99HS-18
2016
1970
28542280
3217
13027
3531
6591395
965
285
549
5733
4467
3833
4678
148
228
209
195
2212
721462
1690
020
163
31CV
J-2-5-3-1-3times
1305
1358
1422
2179
1653
1254
2803
1019
44750
178
254
801
411
6033
4467
3367
4622
312
193
330
278
22269
19547
17250
19689
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
1335
1742
2400
1826
922
5226
12806
6318
236
458
1054
583
5633
4433
3600
4556
321
315
259
298
2210
419524
17087
19572
33CV
J-2-5-3-1-3times
96AC
-93
1552
16803260
2164
166710094
11941
7901250
974
1115
779
5533
4533
446748
44
170
202
317
230
2392
123317
19333
2219
0
34CV
J-2-5-3-1-3times
Togo-II
142216072638
1889
1012
4163
11061
5412
261
377
891
510
6167
4967
4533
5222
207
225
325
252
23590
22860
20278
22243
35CV
J-2-5-3-1-3times
99HS-18
1435
1600
2700
1912
3056
3237
7089
4461449
301
658
469
5700
44673800
4656
148
178
225
183
22444
20240
1696
719884
361305times(77371times
BSEC
TCP
-1)
1499
1767
2058
1774
1882
4665
7920
4822
356
426
724
502
566742673800
4578
271
242
305
273
22011
19295
16417
19241
371305times96AC
-93
16781605
2757
2014
1516
10037
11960
7838
311
11381284
911
560040
673400
4356
182
276
251
236
2396
519625
15800
1979
738
1305timesTo
go-II
1177
16802249
1702
902
4676
9388
4989
241
456
795
497
6533
5533
4733
5600
214
266
296
259
21894
20217
19222
20444
391305times99HS-18
1774
1767
2766
2102
2145
4628
10618
5797
301
447
991
580
5933
4500
3733
4722
128
142
180
150
2275
719413
16967
1971
2
40(77371times
BSEC
TCP
-1)times
96AC
-93
1748
1795
31712238
1537
12586
14379
9501
211
1104
1183
833
546744
00346744
44
127
201
226
185
23294
21089
17858
2074
7
10 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(
days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
41(77371times
BSEC
TCP
-1)times
Togo-II
19652007
2653
2208
2136
5601
9503
5747
315
635
1001
650
5533
4833
410048
22
213
241
286
247
22662
22094
17667
20808
42(77371times
BSEC
TCP
-1)times
99HS-18
15011687
2705
1964
1935
7253
11806
6998
314
704
1067
695
5467
4433
5133
5011
177
167
175
173
22305
19277
16392
19325
4396AC
-93timesTo
go-II1759
1618
2123
1834
1539
10347
12212
8033
325
907
1090
774
5900
450039674789
214
269
339
274
23252
21554
16875
20560
4496AC
-93times
99HS-18
1353
1618
2925
1966
3532
7223
11950
7569
440
654
1024
706
5600
4900
4533
5011
149
175
206
177
24494
20698
18111
2110
145
Togo-IItimes
99HS-18
1117
1278
2582
1659
3448
6764
11910
7374
477
767
1207
817
6000
5133
4333
5156
183
203
216
201
17683
15541
14333
15853
Mean
1513
1618
2492
1874
1656
5773
9854
5761
272
538
885
565
586846
534116
4879
218
233
268
240
21297
19547
16529
1912
4SE
101
073
098
mdash079
152
317
mdash014
014
028
mdash351
365
428
mdash036
039
046
mdash985
1173
1211
mdashCD
284
205
276
mdash221
427
890
mdash038
040
079
mdash12
513
015
2mdash
013
014
016
mdash351
418
431
mdash
(c)
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
1642
1667
1744
1684
1504
1349
1526
1460
2783
2405
2634
2607
836
745
908
830
4287
3754
4160
4067
2H7729-2
1693
1817
1950
1820
1012
859
1016
962
3433
2809
2591
2944
649
597
651
632
4445
3668
3607
3906
3G73-107
2000
2028
2077
2035
2280
1966
2312
2186
4695
4023
4229
4316
1281
1170
1463
1305
6975
5989
6541
6502
477245
1523
1630
1728
1627
1796
2489
2702
2329
5423
4851
4498
4924
748
1464
1743
1319
7219
7341
7200
7253
5CV
J-2-5-3-1-3
2282
2692
2787
2587
2411
2643
2901
2652
5857
4836
5024
5239
1206
1335
1769
1437
8268
7479
7925
7891
61305
1851
1951
2054
1952
2026
1763
1908
1899
4657
4414
4463
4511
1468
1336
1539
1448
6683
6177
6371
6410
777371timesBS
ECTCP
-11629
1645
1733
1669
1244
1692
1884
1607
4925
4183
4435
4515
625
1092
1483
1067
6169
5875
6319
6121
896AC
-93
1379
1519
1600
1499
2066
2942
3088
2698
4501
4095
4229
4275
984
1565
1678
1409
6567
7037
7316
6973
9To
go-II
1904
2057
2103
2021
2167
1844
2358
2123
4520
3446
3904
3957
1298
1138
1502
1313
6687
5290
6262
6080
1099HS-18
1475
1485
1633
1531
3180
2904
3382
3155
5633
4139
5205
4992
1656
1536
1879
1690
8813
7043
8587
8147
1H77833-2timesH7729-2
2010
2046
2133
2063
3645
3075
4377
3699
7675
6540
7199
7138
2014
1708
2880
2201
11320
9615
11576
10837
2H77833-2timesG73-107
2066
2101
2122
2096
5002
4053
4601
4552
7882
7430
6963
7425
3146
2565
2808
2840
12884
11482
11564
11977
3H77833-2times77245
1764
2048
2083
1965
3769
4497
4559
4275
8681
8515
8475
8557
2284
3356
3377
3006
12450
13012
13034
12832
4H77833-2times
CVJ-2-5-3-1-3
2205
2383
2733
2441
3693
5655
5220
4856
11656
10287
1010
510683
2198
4488
4176
3621
15348
1594
215325
15538
5H77833-2times1305
2209
2423
2491
2374
6008
5842
6933
6261
15238
14311
13889
14479
3758
4057
5591
4469
21246
2015
320822
2074
0
6H77833-2times(77371times
BSEC
TCP
-1)
1789
1823
1992
1868
3593
4288
4903
4262
8922
6522
7429
7625
2178
3224
3070
2824
12515
10811
12333
11886
7H77833-2times96AC
-93
1739
1960
2011
1903
4557
4350
4818
4575
7916
6068
7290
7092
2411
2529
3191
2710
12474
10419
1210
811667
8H77833-2timesTo
go-II
1927
2389
2406
2241
4185
5828
6215
5409
12319
10279
11023
11207
2433
4254
4631
3773
16504
1610
717238
16616
9H77833-2times99HS-18
1941
2245
2330
2172
4227
4139
4529
4298
9211
8120
7969
8433
3154
3184
3253
3197
13438
12259
12498
1273
210
H7729-2timesG73-107
1968
2109
2158
2079
3725
3042
3488
3418
7313
5956
6174
6481
2822
2001
2357
2393
11038
8998
9662
9899
Advances in Agriculture 11
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
11H7729-2times77245
1847
2048
2200
2032
2467
3759
5466
3898
7672
7449
8921
8014
1356
2350
3796
2500
1013
911208
14387
11911
12H7729-2times
CVJ-2-5-3-1-3
2218
2585
2767
2523
3674
4372
4483
4176
10053
9467
8664
9395
2417
3312
3345
3025
1372
813839
1314
713571
13H7729-2times1305
2169
2338
2547
2352
6728
6166
6792
6562
13333
11662
13301
1276
54128
4501
5660
4763
20061
17828
20093
19327
14H7729-2times(77371times
BSEC
TCP
-1)
1925
2095
2127
2049
3530
4165
4649
4115
9638
6917
7698
8084
1681
2833
3150
2555
1316
8110
82
12347
1219
915
H7729-2times96AC
-93
2012
2070
2180
2087
4955
4447
4596
4666
8738
6882
7762
7794
3754
3558
3181
3498
13693
11329
12359
12460
16H7729-2timesTo
go-II
2138
2428
2563
2377
4949
4660
5118
4909
8549
7243
7191
7661
3586
3353
3028
3322
13498
11903
12310
12570
17H7729-2times99HS-18
1899
1933
2073
1969
4046
4527
4988
4520
8492
7895
8144
8177
2561
3378
3553
3164
12539
12422
1313
212698
18G73-107times77245
1840
2253
2437
2177
2580
4776
5915
4424
11673
10608
10459
10913
1358
3163
4286
2936
14253
15385
16374
15337
19G73-107times
CVJ-2-5-3-1-3
2324
2537
2691
2517
4958
5030
5599
5196
9524
9395
1114
610022
3241
3645
4444
3776
14483
14425
1674
515217
20G73-107times1305
2228
2433
2583
2415
6340
5794
6373
6169
12490
1015
8119
61
11536
4199
4199
5018
4472
18830
1595
218334
1770
6
21G73-107times(77371times
BSEC
TCP
-1)
1943
2128
2220
2097
3936
3980
5770
4562
11697
9858
10303
10619
1874
2369
4181
2808
15633
13839
16073
1518
222
G73-107times96AC
-93
2118
2218
2317
2217
4270
5843
6201
5438
10319
9872
1015
910117
2703
4460
4379
3847
14589
15715
16359
15555
23G73-107timesTo
go-II
2237
2365
2733
2445
5736
5175
6822
5911
12098
10808
1299
611967
3984
3670
5372
4342
17835
1598
219818
17878
24G73-107times99HS-18
2025
2081
2322
2143
3558
4229
6612
4800
12350
9685
11516
1118
42196
2782
5009
3329
1590
813914
1812
915983
2577245timesCV
J-2-5-3-1-3
2109
2360
2675
2381
6760
6558
7299
6872
15225
11861
1416
21374
94225
4752
5839
4939
2198
518418
21461
20621
2677245times1305
1919
2100
2197
2072
5700
5142
5347
5397
11687
10565
10737
10996
4318
3956
4244
4173
17386
1570
816085
16393
2777245times(77371times
BSEC
TCP
-1)
1851
1935
2020
1936
2577
2460
2838
2625
6989
4778
4636
5468
1631
1662
1999
1764
9566
7238
7474
8093
2877245times96AC
-93
1729
1780
2133
1881
5541
5058
5748
5449
11952
8174
9526
9884
3646
3587
4071
3768
17493
13232
15274
15333
2977245timesTo
go-II
2300
2508
2658
2489
3067
5431
5963
4820
11737
11021
1216
411641
1598
4053
4770
3474
14805
16452
1812
616461
3077245times99HS-18
1982
2019
2223
2075
3846
3119
4225
3730
7493
6720
6476
6896
3205
2578
2643
2809
11339
9839
1070
110626
12 Advances in Agriculture
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
31CV
J-2-5-3-1-3times1305
2406
2553
3067
2675
4178
4796
6281
5085
13822
11425
12252
12500
2472
3241
4907
3540
18000
16222
18532
17585
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
2142
2173
2238
2184
4522
5235
5640
5132
11673
9658
10248
10526
2614
3766
4178
3519
1619
514893
15888
15659
33CV
J-2-5-3-1-3times
96AC
-93
2350
2477
2600
2476
4676
4280
6090
5015
11975
9832
10868
10891
3097
2931
4445
3491
16651
14111
1695
815907
34CV
J-2-5-3-1-3times
Togo-II
2093
2666
2989
2583
4058
3727
5190
4325
12588
10717
11217
11507
2742
2570
4185
3166
16645
14443
16407
15832
35CV
J-2-5-3-1-3times
99HS-18
2028
2311
2386
2242
3287
2513
5356
3719
10055
9733
1036
410051
2004
1581
4251
2612
13342
12246
1572
01376
9
361305times(77371times
BSEC
TCP
-1)
2005
2173
2210
2129
3569
4692
5140
4467
11652
9826
10041
10507
2005
3665
4179
3283
15221
14518
1518
214974
371305times96AC
-93
1822
2003
2047
1957
5115
4023
4813
4650
9965
7675
7571
8404
3456
2647
3155
3086
15080
11698
12384
13054
381305timesTo
go-II
2545
2583
2644
2591
6885
7283
6795
6987
12265
11410
12583
12086
4500
6120
5350
5323
1915
018693
19378
19074
391305times99HS-18
2070
2408
2593
2357
5219
4332
4800
4784
1092
97677
8968
9192
3924
3332
3840
3699
1614
912009
1376
913976
40(77371times
BSEC
TCP
-1)
times96AC
-93
2056
2115
2157
2109
3158
4463
4717
4113
9166
7540
8100
8268
1880
3542
3347
2923
12324
12003
12817
12381
41(77371times
BSEC
TCP
-1)
timesTo
go-II
1868
2253
2450
2190
4422
6458
7038
5973
1415
312369
1379
713439
2291
4646
5586
4174
18575
18827
20835
19412
42(77371times
BSEC
TCP
-1)
times99HS-18
1995
2026
2209
2077
2720
2909
3543
3057
7392
4692
5023
5702
1766
2187
2147
2033
10112
7601
8566
8760
4396AC
-93timesTo
go-II
1953
2027
2511
2164
5972
4391
7126
5830
15537
1392
714432
14632
3780
2538
5655
3991
21510
18318
21558
20462
4496AC
-93times99HS-18
1952
2021
2242
2072
3465
2758
4496
3573
5959
5186
6094
5746
2707
2154
2409
2423
9424
7944
10590
9319
45To
go-IItimes
99HS-18
1932
2008
2658
2199
5381
4995
5436
5270
10299
8876
9244
9473
3986
3842
3668
3832
15680
13871
14680
1474
3Mean
1982
2146
2301
2143
3963
4123
4836
4307
9498
8087
8663
8749
2510
2914
3513
2979
13460
12210
13499
13056
SE243
198
245
mdash671
501
626
mdash959
750
822
mdash423
349
344
mdash1155
927
1167
mdashCD
087
071
087
mdash239
178
223
mdash342
267
293
mdash15
112
412
2mdash
411
330
416
mdash
Advances in Agriculture 13Ta
ble4Analysis
ofvaria
ncefor
10times10
half-diallelfor
different
charactersin
threee
nviro
nments
(a)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Germination
()
Rateof
emergence
STI()
SSTI
()
Num
bero
fleavesseedling
Seedlin
gheight
(cm)
Freshweight
(g)seedlin
gDry
weight
(g)seedlin
g
E 1
Replicates
2652731
596768
783393
20700
93018674
33764
1033999
002590
Treatm
ents
541097
0255lowastlowast
8181153lowastlowast
30863092lowastlowast
20680900lowastlowast
1100708lowastlowast
2106317lowastlowast
930829527lowastlowast
319767lowastlowast
Parents
95118
163lowastlowast
14289925lowastlowast
59114816lowastlowast
18880254lowastlowast
1084227lowastlowast
1087906lowastlowast
22561719lowastlowast
042162lowastlowast
Hybrid
s44
11423573lowastlowast
3398933lowastlowast
25697199lowastlowast
20633716lowastlowast
472874lowastlowast
1391526lowastlowast
674123518lowastlowast
261839lowastlowast
Parentsv
ersush
ybrid
s1
43693104lowastlowast
163619
916lowastlowast
3896838
3896
2793lowastlowast
28873741lowastlowast
4272
2798lowastlowast
234144289lowastlowast
5367022lowastlowast
Error
108
281184
1356832
1173684
674337
040104
306
462
2015
1619
005535
E 2
Replicates
2583819
3707389lowast
1596635
198738
034925
448213
899529
007177
Treatm
ents
549997443lowastlowast
2825177lowastlowast
35378177lowastlowast
19074306lowastlowast
665284lowastlowast
1764
081lowastlowast
257694570lowastlowast
2177519lowastlowast
Parents
96507784lowastlowast
3718
879lowastlowast
69866289lowastlowast
1798
5404lowastlowast
705379lowastlowast
1408518lowastlowast
10612519lowastlowast
077429lowastlowast
Hybrid
s44
10569731lowastlowast
2127302lowastlowast
2910
2537lowastlowast
19429240lowastlowast
329256lowastlowast
7000
03lowastlowast
186026552lowastlowast
1483428lowastlowast
Parentsv
ersush
ybrid
s1
16223710lowastlowast
25488361lowastlowast
1113
326
13257362lowastlowast
15089673lowastlowast
5178
3569lowastlowast
5634825791lowastlowast
51618348lowastlowast
Error
108
334760
1097039
1049505
635570
06764
116
0576
694613
006206
E 3
Replicates
2894398
4808815lowast
mdashmdash
051076
095989
3768421
030220
Treatm
ents
541261560
0lowastlowast
4842216lowastlowast
mdashmdash
2811077lowastlowast
5947316lowastlowast
3870844
50lowastlowast
3445076lowastlowast
Parents
97135
735lowastlowast
1097
6388lowastlowast
mdashmdash
1468699lowastlowast
1112
764lowastlowast
136081650lowastlowast
1307836lowastlowast
Hybrid
s44
13217740lowastlowast
1379360
mdashmdash
15540
71lowastlowast
4581632lowastlowast
252084767lowastlowast
2378032lowastlowast
Parentsv
ersush
ybrid
s1
35440
180lowastlowast
102000335lowastlowast
mdashmdash
70200767lowastlowast
109548362lowastlowast
8586095705lowastlowast
69630188lowastlowast
Error
108
782907
1085444
mdashmdash
139771
291325
3019
169
02400
4(b)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Panicle
emergence
Effectiv
etillersplant
Planth
eight
Earlength
Earw
eightp
lant
Dry
fodd
eryieldplant
Grain
yieldplant
Totalbiological
yieldplant
E 1
Replicates
231860
61lowastlowast
416348lowastlowast
1672
9472lowast
1706
411077192
9634539
392777
115544
59Treatm
ents
544152
368lowastlowast
085657lowastlowast
326706089lowastlowast
1602572lowastlowast
6177
0406lowastlowast
304113
204lowastlowast
34285030lowastlowast
575617
582lowastlowast
Parents
94511481lowastlowast
018538lowastlowast
140807476lowastlowast
2240093lowastlowast
11653
959lowastlowast
27014155lowastlowast
3858650lowastlowast
61321919lowastlowast
Hybrid
s44
3201347lowastlowast
100797lowastlowast
110395657lowastlowast
1010
497lowastlowast
40296671lowastlowast
171271411lowastlowast
2413
6880lowastlowast
302999650lowastlowast
Parentsv
ersush
ybrid
s1
4276
5286lowastlowast
02360
9lowast11517452
616lowastlowast
2191
6167lowastlowast
1457662749lowastlowast
8643043524lowastlowast
7546
41053lowastlowast
17199467546lowastlowast
Error
108
468159
004907
3692099
224610
17114
303498126
682350
5075268
E 2
Replicates
2656364
002556
61575565lowastlowast
828842lowastlowast
589094
2268506
260877
1507116
Treatm
ents
542999618lowastlowast
069822lowastlowast
268998723lowastlowast
2436886lowastlowast
60592784lowastlowast
247259877lowastlowast
4192
0073lowastlowast
513483489lowastlowast
Parents
926300
00lowastlowast
048079lowastlowast
165555
405lowastlowast
3871874lowastlowast
1412
2393lowastlowast
19543682lowastlowast
3132
426lowastlowast
57230706lowastlowast
Hybrid
s44
2533
165lowastlowast
07344
9lowastlowast
9616
2857lowastlowast
1463359lowastlowast
35495366lowastlowast
154759288lowastlowast
26262465lowastlowast
293492304lowastlowast
Parentsv
ersush
ybrid
s1
26850135lowastlowast
105929lowastlowast
880476
6697lowastlowast
32357161lowastlowast
1583112746lowastlowast
6366731560lowastlowast
107994
3646lowastlowast
14299370699lowastlowast
Error
108
507598
005829
5234122
149406
955133
2142411
462311
3273886
E 3
Replicates
2029697
021161
9459847
143746
985141
46800
6492
6334
3345160
Treatm
ents
548045499lowastlowast
094511lowastlowast
192420108lowastlowast
3279225lowastlowast
72049211lowastlowast
298953
021lowastlowast
55061308lowastlowast
651732994lowastlowast
Parents
98010
741lowastlowast
094166lowastlowast
116037343lowastlowast
3702185lowastlowast
16305190lowastlowast
2323644
0lowastlowast
4553
893lowastlowast
71484982lowastlowast
Hybrid
s44
6254377lowastlowast
095514lowastlowast
7378
1513lowastlowast
2188348lowastlowast
31813193lowastlowast
190263916lowastlowast
31572179lowastlowast
368689934lowastlowast
Parentsv
ersush
ybrid
s1
8716
7710lowastlowast
053496lowast
609996
3199lowastlowast
47471212lowastlowast
2344
130203lowastlowast
756272
2861lowastlowast
154314
9707lowastlowast
18327859
719lowastlowast
Error
108
696981
007883
5578505
228608
1491014
2574
223
449588
5187705
lowast
Sign
ificant
at119875=005
lowastlowastSign
ificant
at119875=001
14 Advances in Agriculture
Table 5 Mean performance of the genotypes for membrane thermostability in two environments
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594
Advances in Agriculture 15
Table 5 Continued
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434
Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash
lowastValues are transformed values
0
6
12
18
24
30
36
42
48
54
minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity
G73-10796AC-93CVJ-2-5-3-1-3
Togo-II99HS-18
H77833-2H7729-2772451305
H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305
1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305
H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305
77245 times 1305
77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305
77371 times BSECT CP-1
H77833-2 times G73-107
Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits
moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling
survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The
16 Advances in Agriculture
Table6Po
oled
correla
tioncoeffi
cientamon
gvario
uscharactersin
threee
nviro
nments
Characters
Germination
Rateof
emergence
STI
SSTI
Leaves
seedlin
gSeedlin
gheight
Fresh
weight
Dry
weight
MTS
Panicle
emergence
Effectiv
etillersplant
Plant
height
Earlength
Ear
weightp
lant
Dry
fodd
eryield
plant
Grain
yield
plant
Total
biological
yield
plant
Germination
1000
0357lowastlowast
0353lowastlowast
0590lowastlowast
0230
0373lowastlowast
0293lowast
0260
0276lowastminus0135minus0478lowastlowast
0326lowast
minus0069minus0104
minus0190
minus0145
minus0167
Rateof
emergence
1000
0233
0351lowastlowast
0605lowastlowast
0585lowastlowast
0551lowastlowast
0524lowastlowast
0139minus0593lowastlowast
0032
0517lowastlowast
0217
0388lowastlowast
0361lowastlowast
0348lowastlowast
0377lowastlowast
STI
1000
0837lowastlowast
0241
0129
0081
004
80890lowastlowast
minus0215
minus0211
0030minus0007minus0043
minus0101
minus0111
minus0084
SSTI
1000
0233
0264
0183
0140
0745lowastlowast
minus0247minus0268lowast
0184minus0017minus006
6minus0129
minus0144
minus0112
Num
bero
fleaves
1000
0683lowastlowast
0633lowastlowast
060
4lowastlowast
0176minus0474lowastlowast
0109
0576lowastlowast
0496lowastlowast
064
1lowastlowast
0607lowastlowast
0630lowastlowast
0630lowastlowast
Seedlin
gheight
1000
0703lowastlowast
0663lowastlowast
0031minus0493lowastlowast
minus0115
0735lowastlowast
0360lowastlowast
0526lowastlowast
0520lowastlowast
0515lowastlowast
0532lowastlowast
Freshweight
1000
0957lowastlowast
minus0016
0425lowastlowast
minus0082
0661lowastlowast
0351lowastlowast
0581lowastlowast
0496lowastlowast
0576lowastlowast
0533lowastlowast
Dry
weight
1000minus0033minus0428lowastlowast
minus0016
0634lowastlowast
0354lowastlowast
0620lowastlowast
0509lowastlowast
0614lowastlowast
0554lowastlowast
MTS
1000minus040
minus0177minus0037
0015
minus0074
minus0138
minus0131
minus0120
Panicle
emergence
1000
minus0150minus0269lowast
0022minus0329lowast
minus0337lowast
minus0280lowast
minus0341lowast
Effectiv
etillersplant
1000
minus0012
0170
0366lowastlowast
040
5lowastlowast
0323lowast
0401lowastlowast
Planth
eight
1000
0650lowastlowast
0603lowastlowast
0596lowastlowast
0619lowastlowast
0610lowastlowast
Earlength
1000
0608lowastlowast
0671lowastlowast
064
8lowastlowast
066
4lowastlowast
Earw
eightp
lant
1000
0910lowastlowast
0984lowastlowast
0957lowastlowast
Dry
fodd
eryield
plant
1000
0907lowastlowast
0991lowastlowast
Grain
yield
plant
1000
0950lowastlowast
Totalbiological
yield
plant
1000
lowastSign
ificant
at119875=005lowastlowastSign
ificant
at119875=001
Advances in Agriculture 17
faster germination and early vigour in seedling could act asphysical indicators for heat tolerance
Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits
Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics
Conflict of Interests
The authors declare that there is no conflict of interestsregarding the publication of this paper
References
[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981
[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993
[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006
[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993
[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013
[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007
[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965
[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011
[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001
[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009
[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962
[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967
[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963
[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001
Submit your manuscripts athttpwwwhindawicom
Nutrition and Metabolism
Journal of
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Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
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The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014
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Applied ampEnvironmentalSoil Science
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AgricultureAdvances in
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BiodiversityInternational Journal of
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GenomicsInternational Journal of
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4 Advances in Agriculture
heat stress were noted in each genotype on daily basis Obser-vations continued till the surviving seedlingswere established(15 DAE) and there was no further mortality Moreover thesoil moisture was depleting near to the permanent wiltingpoint and drought stress could also come into play along withheat stress to further continue the experiment Since experi-ment proceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedlingsurvival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of heat stress at seedling stage was observed on latergrowth stages
231 Thermotolerance Characteristics For heat tolerancecharacteristics 3 indices namely seedling thermotoleranceindex (STI) [4] seed to seedling thermotolerance index(SSTI) [8] and membrane thermostability (MTS) wereapplied to evaluate the hybrids and parents STI was cal-culated as the ratio of number of seedlings survived to thenumber of seedling emergences expressed in percentageSeed to seedling thermotolerance index (SSTI) was calculatedas the ratio of seedling survival to the number of seedlingsexpected to emerge expressed in percentage [8] The SSTIis an extension of STI by taking expected germination intoaccount It was necessary to correct the effect of under soilmortality (USM) The loss of germination due to heat (ieunder soil mortality) can be measured only after a standardgermination test Therefore the germination of seed fromthe same lot under monsoon environment (main pearl milletgrowing season where no under soil mortality occurreddue to heat stress) was taken as expected germination forcalculation of SSTI
232 Membrane Thermostability These genotypes were alsoscreened through laboratory technique (membrane ther-mostability) [9 10] The young leaves of 21-day-old seedlingsof each genotype in three replication plots were used formembrane thermostability test A middle portion of 10 cmfrom each leaf was taken and the midrib was removed andtwo equal halves of the 10 cm each were made Each halfwas taken to provide replications Each leaf portion was cutinto pieces and thoroughly washed and rinsed with distilledwater two times to remove the electrolytes adhering to theplant tissue as well as electrolyte released from the cutting ofplant tissue and put them into test tubes After final rinsingtubes were drained maintaining sufficient water to preventdesiccation of plant material during heat treatment Thesetest tubes were covered with aluminium foil and incubated inwater bath at 55∘C for 15 minutes for giving heat treatmentAfter the heat treatment period 10mL of distilled waterwas added to the test tubes Then all the test tubes wereheld at 10∘C overnight to allow diffusion of electrolytes fromplant material Test tubes were brought out of refrigeratorand shaken well to mix the contents An initial electricalconductivity (EC) of both replications of each treatment (119879
1)
was determined with electrical conductivity meter Thenthese test tubes were autoclaved at 15 psi for 2 minutes forcomplete electrolyte leakage from the leaf samples These testtubes were then cooled at room temperature and contentsmixed and final EC was taken for all the treatments (119879
2)
Membrane thermostability (MTS) was calculated accordingto Ibrahim and Quick [9] and Yadav et al [10] as follows
Membrane Thermostability (MTS) = (1 minus1198791
1198792
) times 100
(2)
where 1198791is conductivity reading after heat treatment and 119879
2
is conductivity reading after autoclavingCompletely randomized design (CRD) for membrane
thermostabilitywas conducted to detect variations among thegenotypes
233 Morphological Characteristics The seedling growthcharacteristics namely germination () emergence rate(ER) number of leavesseedling (two-week stage) seedlingheight (two-week stage) seedling fresh weight (g) (four-week stage) and seedling dry weight (g) (four-week stage)were recorded The data for seedling height and number ofleaves per seedling was recorded on 10 random seedlings perplot Emergence rate (an index for speed of germination)can be used as a tool in pearl millet breeding programs forevaluation of seedling vigour Speed of germination is oneof the oldest concepts of seedling vigour The emergencerate (ER) was calculated by dividing the number of normalseedlings obtained at each counting by the number of daysseeds had been in the seed bed (DAS)The values obtained ateach count were then summed at the end of the germinationtest to obtain the emergence rate [11]
Emergence Rate (ER) =Seedling count
Days to first count+ sdot sdot sdot
+
Seedling countDays to final count
(3)
After termination of the heat stress on the seedlings byirrigating the field the experiment was continued to studythe effect of seedling heat stress on maturity traits on thesurvived plants The maturity traits observed were panicleemergence effective tillersplant plant height ear length earweightplant dry fodder yieldplant grain yieldplant andtotal biological yieldplant
24 Statistical Analysis Analysis of variance for randomizedblock design (RBD) for field traits that is germinationemergence rate number of leavesseedling seedling heightseedling fresh weight and seedling dry weight and com-pletely randomized design (CRD) for membrane thermosta-bility test was conducted under each environment on meanbasis [12] Analyses were done using the software OPSTATdeveloped at CCS HAU Hisar Correlations among the vari-ous characters studied were calculated to establish the natureand magnitude of associations among the traits [8 13] with
Advances in Agriculture 5
the software SPSS (SPSS Inc) Multivariate clustering wasdone based on paired group cluster method using similaritycoefficient under Past 140 software [14] Multivariate analysisbased on the Euclidean distancematrix was estimated amonggenotypes to distinguish genotypes from each other Thegrouping of genotypes was generated using paired groupcluster method
3 Results and Discussion
The excessive heat (soil temperature up to 630∘C in E2)
about 18∘C higher than the normal atmospheric temperature(Figures 1(a) and 1(b)) was responsible for mortality ofseedlings Many seedlings were found desiccated at the pointof contact besides the desiccation at the tipsThe temperaturearound the tip level (5 cm above the soil surface) also reachedup to 50∘C (Figure 1(c)) It could therefore be concluded thatthe seedlings had to survive in a really high temperature Themoisture level remained above the permanent wilting point(Figure 1(d)) therefore drought effect was not in the playThe heat tolerance was measured as STI (Table 3) throughthe field screening technique of Peacock et al [4] Anotherindex known as SSTI was also calculated as an improvementand extensionThe analysis of variance (RBD) for 8 charactersin 3 environments showed significant genotypic differencesNo variability was observed for STI and SSTI in E
3only In
this environment no seedling mortality was observed andthe germination was taken as standard The values for STIand SSTI were therefore equal and the highest for all thegenotypes in this environment and hence no variation wasobserved
The hybrids were evaluated on the basis of mean perfor-mance for various characters in each environment (Table 3)On the mean basis STI value ranged from 5332 to 8224with an average of 7072 The hybrid H7729-2 times CVJ-2-5-3-1-3 was the best scorer for heat tolerance followed byH77833-2 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II The hybrid H77833-2 times 1305 was found susceptible toheat stress Seed to seedling thermotolerance index (SSTI)which is expected to be more rigorous index deviated thevalues from STI according to variation in germination undernormal environment (E
3) as the SSTI also coversincludes
the under surface mortality (USM) during germinationThe reductions in values were not strictly proportional tothe STI The reduction is dependent on thermosensitivityof germination We also came across such hybrids whichshowed poor germination and high STI SSTI values rangedfrom 4605 to 7287 with an average of 6090 The genotypeH77833-2 times 96AC-93 had the highest worth for SSTI onmean basis followed byG73-107times (77371timesBSECTCP-1) andH77833-2 times (77371 times BSECT CP-1) (Table 3) The analysisof variance for ten different characters in three differentenvironments (two stressed and one normal) was carried outand the results showed significant genotypic differences forall the characters (Table 4)
A caution is needed for evaluating such genotypes underSTI The SSTI gave a highly reduced value putting thesecrosses in the susceptible list It may be concluded therefore
that SSTI is more dependable and desirable tool for screeningand evaluating the genotypes for heat tolerance The germi-nation was lower in E
2than in E
1 This period has the highest
range of temperature with a high average Genotype-wise thehighest germination (6209) was recorded for the genotypeH7729-2 times (77371 times BSECT CP-1) on mean basis whichgives a preliminary indication of resistance to heat while thegenotype 1times 6 had the lowest germination (3533)The hybridH77833-2 times G73-107 was adjudged as the fastest emergenceof seedlingsThe genotype (77371timesBSECTCP-1)times 96AC-93had the highest fresh weightseedlingThe genotype H7729-2 times Togo-II had the highest seedling dry weight E
1had lower
dry weightseedling than E2indicating the more heat stress
during the E1
31 Membrane Thermostability Membrane thermostability(MTS) has long been recognised as a parameter of heattolerance All the hybrids were put to membrane thermosta-bility test in the laboratory under standard protocol Acompletely randomised design (CRD) analysis was carriedout for membrane thermostability (MTS) The mean sum ofsquares due to genotypes was highly significant indicatingthat enough genetic variability was present for membranethermostability (MTS) in each environment which justifiedfurther analysis of the data The range of values lay between5073 and 7742 Among the parents H77833-2 G73-107CVJ-2-5-3-1-3 77371 times BSECT CP-1 96AC-93 Togo-II and99HS-18 were verified as resistant genotypes and H7729-2 77245 and 1305 genotypes were found susceptible asmentioned in Table 1 (on the basis of previous experiment)Among all the hybrids H7729-2 times CVJ-2-5-3-1-3 CVJ-2-5-3-1-3 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II werethree highly tolerant genotypes on the basis of membranethermostability (Table 5)
32 Correlations The pooled correlations among the charac-ters were calculated (Table 6) around heat tolerance indices(STI SSTI and membrane thermostability) with a view tofind whether there was any association of these characterswith seedling as well as maturity traits It was observedthat STI and SSTI recorded the highest significant cor-relation among themselves as they are related indices forheat tolerance Membrane thermostability also showed highsignificant correlation with STI as well as SSTI STI hada significant positive association with germination of seedonly among seedling and maturity traits studied SSTI inaddition was also positively correlated with germination andemergence rate and negatively associated with number ofeffective tillersplantMembrane thermostability also showedsignificant positive correlation with seed germination amongseedling and maturity traits studied While evaluating thediallel progenies it was also seen that the heat toleranceindices STI SSTI and membrane thermostability were notshowing any perceptible correlation with the rest of thedevelopmental traits (seedling andmaturity traits)The stressperiod was terminated by irrigating the field experiment after15 days fromdate of sowingwhen the seedlings get establishedand further mortality stopped due to heat Moreover the soil
6 Advances in Agriculture
Table3Perfo
rmance
ofthep
arentsandthec
rosses
ford
ifferentcharacters
(a)
Sr
No
Genotypes
Germination(
)Ra
teof
emergencelowast
STIlowast
()
SSTIlowast(
)Num
bero
fleavesseedlin
gE 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
3941
3737
6164
4614
4037
4109
4279
4142
7830
7387
9000
8072
4498
4133
9000
5877
661
502
800
654
2H7729-2
5513
4726
6092
5443
2815
4980
3258
3684
4835
4495
9000
6110
4441
3602
9000
5681
363
294
427
361
3G73-107
5037
4826
5918
5260
4534
4132
4481
4382
6187
5413
9000
6866
5213
4460
9000
6224
878
710
935
841
477245
4747
4624
5565
4978
3625
4604
4047
4092
4385
3580
9000
5655
3803
3040
9000
5281
377
292
437
369
5CV
J-2-5-3-1-3
4824
4832
6897
5518
5044
4907
4960
4970
7193
6813
9000
7669
4959
4785
9000
6248
802
580
923
768
61305
4326
3891
5404
4540
3376
5056
3573
4002
3903
3281
9000
5395
3211
2452
9000
4888
350
284
414
349
777371timesBS
ECTCP
-14747
4502
6320
5190
4934
5075
5065
5025
6952
6706
9000
7552
5056
4671
9000
6242
621
542
700
621
896AC
-93
4651
3818
5722
4730
4312
4915
4753
4660
6660
6001
9000
7220
5212
3920
9000
6044
701
590
899
730
9To
go-II
4613
3937
5276
4609
4245
4737
4797
4593
7198
6861
9000
7686
5980
4767
9000
6582
681
629
853
721
1099HS-18
4575
3823
6247
4882
4317
4659
4746
4574
7594
7319
9000
7971
5154
4172
9000
6109
745
554
915
738
1H77833-2timesH7729-2
5787
5295
6733
5938
4964
5015
4989
4989
6803
6504
9000
7436
5808
5134
9000
6647
711
585
780
692
2H77833-2timesG73-107
5472
4417
6405
5431
5448
5422
5239
5370
7231
6769
9000
7667
5980
4556
9000
6512
1101
705
1340
1049
3H77833-2times77245
4076
3707
5504
4429
5217
5446
5238
5300
6627
6224
9000
7284
4690
4037
9000
5909
740
525
965
744
4H77833-2timesCV
J-2-5-3-1-3
4843
4300
5856
5000
4862
4956
4913
4911
7061
6519
9000
7527
5575
4632
9000
6403
998
885
1258
1047
5H77833-2times1305
3296
2780
4521
3533
5076
4907
5238
5074
3732
3265
9000
5332
2765
2050
9000
4605
1192
934
1600
1242
6H77833-2times(77371times
BSEC
TCP
-1)5636
5539
6258
5811
4489
5219
5015
4908
6981
6482
9000
7488
6146
5680
9000
6942
898
726
1525
1050
7H77833-2times96AC
-93
5720
5515
6335
5857
4800
5186
5075
5021
7833
7786
9000
8206
6603
6256
9000
7287
1107
873
1193
1058
8H77833-2timesTo
go-II
4862
3453
5709
4675
5036
4829
4911
4925
6190
5500
9000
6897
5214
3339
9000
5851
979
670
1107
919
9H77833-2times99HS-18
4575
4418
5447
4813
5041
5074
5006
5040
5800
5073
9000
6625
4846
4133
9000
5993
978
733
1135
949
10H7729-2timesG73-107
5615
4962
6273
5617
5023
5535
5165
5241
6143
5501
9000
6881
5540
4441
9000
6327
1000
720
1363
1028
11H7729-2times77245
5472
5164
6281
5639
4745
5031
5028
4935
5572
5175
9000
6582
4901
4345
9000
6082
880
620
897
799
12H7729-2timesCV
J-2-5-3-1-3
5494
5124
6536
5718
4804
5316
4957
5026
7596
8076
9000
8224
6093
5680
9000
6924
981
932
1451
1121
13H7729-2times1305
3804
3826
5511
4380
3596
4724
4387
4236
6362
7075
9000
7479
4230
4517
9000
5916
926
725
1497
1049
14H7729-2times(77371times
BSEC
TCP
-1)
6369
4847
7412
6209
4847
5351
5246
5148
6377
6031
9000
7136
5683
4230
9000
6304
977
671
1380
1009
15H7729-2times96AC
-93
5314
4657
6702
5558
4514
5295
5102
4971
5612
4856
9000
6489
4613
3603
9000
5739
846
679
1397
974
16H7729-2timesTo
go-II
5827
5594
6651
6024
5072
5279
5271
5207
6563
5371
9000
6978
5763
4652
9000
6472
873
719
1040
877
17H7729-2times99HS-18
5741
4638
7918
6099
4847
5024
5021
4964
6179
5603
9000
6927
4882
3725
9000
5869
1012
730
1600
1114
18G73-107times77245
5115
4646
6103
5288
4977
5242
5450
5223
7006
6063
9000
7356
5685
4594
9000
6426
1021
790
1292
1034
19G73-107timesCV
J-2-5-3-1-3
5134
4894
6427
5485
4901
5317
5181
5133
6637
6124
9000
7254
5253
4710
9000
6321
1039
863
1254
1052
20G73-107times1305
4881
4561
7597
5680
4717
4791
4775
4761
6704
6380
9000
7361
4556
4115
9000
5890
1140
882
1657
1226
21G73-107times(77371times
BSEC
TCP
-1)
5636
5519
6283
5812
4986
5219
5203
5136
7990
6203
9000
7731
6620
5436
9000
7019
977
703
1395
1025
22G73-107times96AC
-93
5740
4510
6415
5555
5129
4982
5195
5102
6978
5556
9000
7178
6156
4019
9000
6392
1077
860
1264
1067
23G73-107timesTo
go-II
4460
4357
6044
4953
4396
5138
4951
4829
5279
4835
9000
6371
4018
3603
9000
5540
906
881
1189
992
24G73-107times99HS-18
5473
4356
6374
5401
5102
4955
5055
5037
6460
5197
9000
6886
5534
3704
9000
6079
1171
766
1635
1191
2577245timesCV
J-2-5-3-1-3
4766
4666
5921
5117
5170
4918
4900
4996
5928
5859
9000
6929
4785
4613
9000
6133
984
920
1194
1033
2677245times1305
4575
4060
6052
4895
5128
5297
5203
5210
5102
4990
9000
6364
3979
3464
9000
5481
1279
823
1550
1217
2777245times(77371times
BSEC
TCP
-1)
5017
4929
6022
5322
5108
5473
5274
5285
5308
5044
9000
6451
4498
4191
9000
5896
833
588
939
787
Advances in Agriculture 7
(a)Con
tinued
Sr
No
Genotypes
Germination(
)Ra
teof
emergencelowast
STIlowast
()
SSTIlowast(
)Num
bero
fleavesseedlin
gE 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
2877245times96AC
-93
5472
4705
622154
66
5135
5197
5006
5113
5247
4843
9000
6363
4708
3784
9000
5831
795
662
1058
838
2977245timesTo
go-II
5252
4492
5889
5211
5188
4861
5074
5041
4885
4263
9000
6049
4421
3379
9000
5600
977
751
1243
990
3077245times99HS-18
5740
4099
6872
5570
5249
4898
5258
5135
5628
4616
9000
6415
4863
3014
9000
5626
1045
863
1184
1031
31CV
J-2-5-3-1-3times1305
4364
3944
5700
4669
4974
4517
4650
4714
6097
5579
9000
6892
4594
3842
9000
5812
936
848
1403
1063
32CV
J-2-5-3-1-3times(77371times
BSEC
TCP
-1)4345
3808
6774
4976
5272
4996
5035
5101
6650
5766
9000
7139
4288
3381
9000
5556
800
712
1013
841
33CV
J-2-5-3-1-3times96AC
-93
4862
4197
5743
4934
4884
4980
5058
4974
7477
6760
9000
7745
5957
4709
9000
6555
933
738
1478
1050
34CV
J-2-5-3-1-3timesTo
go-II
4670
4343
6233
5082
4399
4813
4631
4614
5806
6614
9000
7140
4422
4479
9000
5967
963
855
1180
999
35CV
J-2-5-3-1-3times99HS-18
5493
5080
7273
5949
5276
4984
5162
5141
7322
6590
9000
7637
5534
4766
9000
6433
998
807
1338
1047
361305times(77371times
BSEC
TCP
-1)
4613
4539
5717
4956
4986
4955
5080
5007
5137
4870
9000
6336
4210
3936
9000
5715
863
630
913
802
371305times96AC
-93
5473
5177
6830
5827
5224
4779
4919
4974
7117
6965
9000
7694
5640
5220
9000
6620
875
694
1476
1015
381305timesTo
go-II
4230
3420
5262
4304
4309
456144
45
4438
5781
4373
9000
6385
4575
2887
9000
5487
637
566
794
666
391305times99HS-18
5433
4507
6947
5629
5225
4753
4991
4989
7287
6968
9000
7752
5576
4479
9000
6352
1115
818
1410
1114
40(77371times
BSEC
TCP
-1)times
96AC
-93
5595
4429
7461
5828
5216
4955
5121
5097
7087
6368
9000
7485
5432
4038
9000
6157
955
753
1462
1057
41(77371times
BSEC
TCP
-1)times
Togo-II
5252
4728
6106
5362
5492
4808
5132
5144
8025
7505
9000
8177
6310
5413
9000
6908
859
693
990
848
42(77371times
BSEC
TCP
-1)times
99HS-18
5595
4298
6295
5396
4729
4887
5031
4883
7366
6945
9000
7770
6312
4556
9000
6623
903
622
1125
883
4396AC
-93timesTo
go-II
5017
3977
6486
5160
5019
5195
5237
5150
5922
5486
9000
6803
4689
3484
9000
5724
1007
728
1167
967
4496AC
-93times99HS-18
5595
4812
6924
5777
5036
5385
5205
5209
7411
5378
9000
7263
5873
3979
9000
6284
1013
652
1334
1000
45To
go-IItimes
99HS-18
4670
4497
7293
5487
4718
4319
4796
4611
5688
5431
9000
6706
3980
3665
9000
5548
939
653
1462
1018
Mean
5043
4482
6271
5265
4792
498149
23
4899
6377
584190
00
7072
5079
4190
9000
6090
898
701
1168
922
SE097
106
161
mdash213
191
190
mdash19
818
7mdash
mdash14
914
6mdash
mdash036
047
068
mdashCD
272
296
453
mdash597
537
534
mdash555
525
mdashmdash
421
409
mdashmdash
103
133
192
mdashlowastTransfo
rmed
data
(b)
Sr
No
Genotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
908
785
2245
1313
859
1537
1924
1440
154
143
160
152
6267
4800
3833
4967
212
227
249
229
15644
15458
13267
1479
02
H7729-2
1125
1175
1789
1363
317
2400
2811
1843
093
191
252
179
6700
5333
5100
5711
185
210
234
210
18850
18565
15389
17601
3G73-107
1166
1475
1966
1536
943
1744
4945
2544
143
129
366
213
6700
5433
5300
5811
218
219
238
225
15009
1496
513503
14492
477245
1438
1373
2085
1632
607
1863
5957
2809
117
180
509
269
6100
4800
5100
5333
232
221
253
235
14036
13802
11742
1319
35
CVJ-2-5-3-1-3
1214
1437
1807
1486
1163
1610
4166
2313
171
141
407
240
6300
4933
4600
5278
239
227
270
245
1994
317261
1497
017391
61305
858
1137
1737
1244
540
967
4861
2123
125
088
417
210
6533
5033
4800
5456
244
213
257
238
15527
15405
12274
14402
8 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
777371timesBS
ECT
CP-1
1377
1487
1892
1585
928
2660
3330
2306
150
258
301
236
5733
4533
4700
4989
242
220
293
252
1513
814550
1315
014279
896AC
-93
1303
1259
1852
1471
1056
1445
5972
2825
185
147
586
306
5500
4833
3833
4722
256
304
318
293
15577
14032
10000
13203
9To
go-II
1056
1220
2272
1516
924
1466
9113
3834
146
129
883
386
6167
4533
4600
5100
185
149
124
153
14578
12073
10033
12228
1099HS-18
1277
1075
1808
1387
1241
2833
7070
3715
228
226
615
356
6100
5000
4167
5089
243
173
324
247
12627
10355
1017
311052
1H77833-2times
H7729-2
1432
1498
2233
1721
1350
4405
8373
4709
159
499
782
480
5767
4433
3800
4667309
290
248
282
21010
18071
15847
18309
2H77833-2times
G73-107
1675
1635
2205
1838
1236
3211
5789
3412
134
313
547
331
5833
4767
3833
4811
262
295
236
265
21253
20449
15808
1917
0
3H77833-2times
77245
1577
1522
2263
1787
1058
6933
8719
5570
255
553
782
530
5267
4633
4600
4833
273
201
265
246
2074
119511
15208
18487
4H77833-2times
CVJ-2-5-3-1-3
1791
1757
2165
1904
20218030
5276
5109
354
779
477
537
5533
4233
3767
4511
165
330
351
282
21827
20072
17110
19670
5H77833-2times1305
1700
1842
28412128
1427
6228
9134
5596
570
579
813
654
5800
4267
3333
4467340
426
451
406
19816
19444
16037
18432
6H77833-2times
(77371times
BSEC
TCP
-1)
1453
1515
3120
2029
789
465510428
5291
211
408
858
492
5233
4167
3267
4222
203
283
267
251
20677
1913
816470
1876
2
7H77833-2times
96AC
-93
1835
1783
2427
2015
2532
6649
1610
684
29
350
650
1473
824
5467
4233
3700
4467243
239
256
246
22278
20434
15735
19482
8H77833-2times
Togo-II
1564
15632500
1876
1882
4772
11660
6105
296
469
972
579
5667
4533
440048
67
169
260
211
213
2412
82375
619389
22424
9H77833-2times
99HS-18
159318002049
1814
1571
480214024
6799
203
544
1547
765
6033
460040
0048
78
261
225
256
247
23466
21081
1793
320
827
10H7729-2times
G73-107
14951568
2075
1713
959
5157
8517
4877
218
382
666
422
6133
5067
4833
5344
300
273
239
271
23651
2191
717083
20884
11H7729-2times77245
1557
1537
2613
1902
1851
5756
5729
4445366
562
524
484
5633
4800
4633
5022
133
233
297
221
1979
219914
15250
18319
12H7729-2times
CVJ-2-5-3-1-3
18442033
28932257
1651
806114885
8199
264
597
1054
638
6333
4600
4233
5056
170
211
217
199
24629
23339
1970
022556
13H7729-2times1305
1325
1677309920
34
1756
724314048
7682
318
635
1232
728
630046
67
3633
4867298
261
361
307
21666
19371
17422
19486
14H7729-2times(77371
timesBS
ECTCP
-1)
1958
1887
3105
2317
1118
4675
14411
6734
242
457
1318
672
5900
4500
3800
4733
164
209
240
204
23661
1995
419250
20955
15H7729-2times
96AC
-93
1512
1717
2779
2002266844
08
11393
6156
356
500
1211
689
5633
4533
4200
4789
221
195
282
232
2194
921635
17213
20266
Advances in Agriculture 9
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
16H7729-2times
Togo-II
1519
1738
2159
1805
2437
9867
1616
294
89
361
961
1527
950
6167
4700
4200
5022
234
272
230
246
26338
2414
520553
23678
17H7729-2times
99HS-18
1938
1787
3151
2292
1574
6218
11318
6370
290
606
956
617
6067
4767
406749
67
172
210
199
194
24689
21854
1778
921444
18G73-107times77245
1856
1813
2210
1960
2435
8550
7828
6271338
633
612
528
5500
4633
440048
44
199
255
307
254
1993
619277
16095
18436
19G73-107times
CVJ-2-5-3-1-3
1517
1769
2407
1897
1556
7728
9136
6140
239
876
917
677
5900
4300
3400
4533
233
266
315
272
23418
22333
19317
21689
20G73-107times1305
1217
1507
2832
1852
1777
5189
1114
960
38
289
588
1197
691
6733
5000
4233
5322
286
210
312
269
2078
81992
817067
19261
21G73-107times(77371
timesBS
ECTCP
-1)
18551946
2730
2177
1801
3482
10415
5233
239
277
753
423
5500
4467
3433
4467243
197
273
238
23817
20263
17425
20502
22G73-107times
96AC
-93
1718
1840
28612140
1345
9333
14669
8449
297
845
1278
807
5600
5033
4633
5089
270
243
300
271
28537
21461
16875
22291
23G73-107timesTo
go-II
168018083087
2192
1112
4678
9319
5036
186
473
985
548
6033
4767
4200
5000
210
209
231
217
24864
24609
21625
23699
24G73-107times99HS-18
1398
1675
3370
2148
2753
8672
11918
7781
362
691
981
678
566746
00
3500
4589
170
247
270
229
24677
20567
17855
21033
2577245times
CVJ-2-5-3-1-3
1500
1728
2203
1810
2282
4679
11492
6151
328
424
1116
623
6000
4333
420048
44300
231
370
300
21257
20497
1772
519826
2677245times1305
143815772080
1698
949
10439
12053
7814
121
915
992
676
5767
4800
3500
4689
168246
316
244
1978
91779
015517
17699
2777245times(77371times
BSEC
TCP
-1)
150516972846
2016
3222
8518
12621
8120
469
827
1141
812
5400
4367
3833
4533
196
225
242
221
2092
819485
1719
219202
2877245times96AC
-93
1653
1797
2117
1856
1934
6056
11543
6511
352
563
911
608
5333
4267
3833
4478
186
169
198
184
22003
19054
1676
719275
2977245timesTo
go-II
1723
1718
3163
2201
1749
8250
15443
8481229
806
1412
816
5967
4700
4067
4911
174
245
298
239
22075
23503
19875
21818
3077245times99HS-18
2016
1970
28542280
3217
13027
3531
6591395
965
285
549
5733
4467
3833
4678
148
228
209
195
2212
721462
1690
020
163
31CV
J-2-5-3-1-3times
1305
1358
1422
2179
1653
1254
2803
1019
44750
178
254
801
411
6033
4467
3367
4622
312
193
330
278
22269
19547
17250
19689
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
1335
1742
2400
1826
922
5226
12806
6318
236
458
1054
583
5633
4433
3600
4556
321
315
259
298
2210
419524
17087
19572
33CV
J-2-5-3-1-3times
96AC
-93
1552
16803260
2164
166710094
11941
7901250
974
1115
779
5533
4533
446748
44
170
202
317
230
2392
123317
19333
2219
0
34CV
J-2-5-3-1-3times
Togo-II
142216072638
1889
1012
4163
11061
5412
261
377
891
510
6167
4967
4533
5222
207
225
325
252
23590
22860
20278
22243
35CV
J-2-5-3-1-3times
99HS-18
1435
1600
2700
1912
3056
3237
7089
4461449
301
658
469
5700
44673800
4656
148
178
225
183
22444
20240
1696
719884
361305times(77371times
BSEC
TCP
-1)
1499
1767
2058
1774
1882
4665
7920
4822
356
426
724
502
566742673800
4578
271
242
305
273
22011
19295
16417
19241
371305times96AC
-93
16781605
2757
2014
1516
10037
11960
7838
311
11381284
911
560040
673400
4356
182
276
251
236
2396
519625
15800
1979
738
1305timesTo
go-II
1177
16802249
1702
902
4676
9388
4989
241
456
795
497
6533
5533
4733
5600
214
266
296
259
21894
20217
19222
20444
391305times99HS-18
1774
1767
2766
2102
2145
4628
10618
5797
301
447
991
580
5933
4500
3733
4722
128
142
180
150
2275
719413
16967
1971
2
40(77371times
BSEC
TCP
-1)times
96AC
-93
1748
1795
31712238
1537
12586
14379
9501
211
1104
1183
833
546744
00346744
44
127
201
226
185
23294
21089
17858
2074
7
10 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(
days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
41(77371times
BSEC
TCP
-1)times
Togo-II
19652007
2653
2208
2136
5601
9503
5747
315
635
1001
650
5533
4833
410048
22
213
241
286
247
22662
22094
17667
20808
42(77371times
BSEC
TCP
-1)times
99HS-18
15011687
2705
1964
1935
7253
11806
6998
314
704
1067
695
5467
4433
5133
5011
177
167
175
173
22305
19277
16392
19325
4396AC
-93timesTo
go-II1759
1618
2123
1834
1539
10347
12212
8033
325
907
1090
774
5900
450039674789
214
269
339
274
23252
21554
16875
20560
4496AC
-93times
99HS-18
1353
1618
2925
1966
3532
7223
11950
7569
440
654
1024
706
5600
4900
4533
5011
149
175
206
177
24494
20698
18111
2110
145
Togo-IItimes
99HS-18
1117
1278
2582
1659
3448
6764
11910
7374
477
767
1207
817
6000
5133
4333
5156
183
203
216
201
17683
15541
14333
15853
Mean
1513
1618
2492
1874
1656
5773
9854
5761
272
538
885
565
586846
534116
4879
218
233
268
240
21297
19547
16529
1912
4SE
101
073
098
mdash079
152
317
mdash014
014
028
mdash351
365
428
mdash036
039
046
mdash985
1173
1211
mdashCD
284
205
276
mdash221
427
890
mdash038
040
079
mdash12
513
015
2mdash
013
014
016
mdash351
418
431
mdash
(c)
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
1642
1667
1744
1684
1504
1349
1526
1460
2783
2405
2634
2607
836
745
908
830
4287
3754
4160
4067
2H7729-2
1693
1817
1950
1820
1012
859
1016
962
3433
2809
2591
2944
649
597
651
632
4445
3668
3607
3906
3G73-107
2000
2028
2077
2035
2280
1966
2312
2186
4695
4023
4229
4316
1281
1170
1463
1305
6975
5989
6541
6502
477245
1523
1630
1728
1627
1796
2489
2702
2329
5423
4851
4498
4924
748
1464
1743
1319
7219
7341
7200
7253
5CV
J-2-5-3-1-3
2282
2692
2787
2587
2411
2643
2901
2652
5857
4836
5024
5239
1206
1335
1769
1437
8268
7479
7925
7891
61305
1851
1951
2054
1952
2026
1763
1908
1899
4657
4414
4463
4511
1468
1336
1539
1448
6683
6177
6371
6410
777371timesBS
ECTCP
-11629
1645
1733
1669
1244
1692
1884
1607
4925
4183
4435
4515
625
1092
1483
1067
6169
5875
6319
6121
896AC
-93
1379
1519
1600
1499
2066
2942
3088
2698
4501
4095
4229
4275
984
1565
1678
1409
6567
7037
7316
6973
9To
go-II
1904
2057
2103
2021
2167
1844
2358
2123
4520
3446
3904
3957
1298
1138
1502
1313
6687
5290
6262
6080
1099HS-18
1475
1485
1633
1531
3180
2904
3382
3155
5633
4139
5205
4992
1656
1536
1879
1690
8813
7043
8587
8147
1H77833-2timesH7729-2
2010
2046
2133
2063
3645
3075
4377
3699
7675
6540
7199
7138
2014
1708
2880
2201
11320
9615
11576
10837
2H77833-2timesG73-107
2066
2101
2122
2096
5002
4053
4601
4552
7882
7430
6963
7425
3146
2565
2808
2840
12884
11482
11564
11977
3H77833-2times77245
1764
2048
2083
1965
3769
4497
4559
4275
8681
8515
8475
8557
2284
3356
3377
3006
12450
13012
13034
12832
4H77833-2times
CVJ-2-5-3-1-3
2205
2383
2733
2441
3693
5655
5220
4856
11656
10287
1010
510683
2198
4488
4176
3621
15348
1594
215325
15538
5H77833-2times1305
2209
2423
2491
2374
6008
5842
6933
6261
15238
14311
13889
14479
3758
4057
5591
4469
21246
2015
320822
2074
0
6H77833-2times(77371times
BSEC
TCP
-1)
1789
1823
1992
1868
3593
4288
4903
4262
8922
6522
7429
7625
2178
3224
3070
2824
12515
10811
12333
11886
7H77833-2times96AC
-93
1739
1960
2011
1903
4557
4350
4818
4575
7916
6068
7290
7092
2411
2529
3191
2710
12474
10419
1210
811667
8H77833-2timesTo
go-II
1927
2389
2406
2241
4185
5828
6215
5409
12319
10279
11023
11207
2433
4254
4631
3773
16504
1610
717238
16616
9H77833-2times99HS-18
1941
2245
2330
2172
4227
4139
4529
4298
9211
8120
7969
8433
3154
3184
3253
3197
13438
12259
12498
1273
210
H7729-2timesG73-107
1968
2109
2158
2079
3725
3042
3488
3418
7313
5956
6174
6481
2822
2001
2357
2393
11038
8998
9662
9899
Advances in Agriculture 11
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
11H7729-2times77245
1847
2048
2200
2032
2467
3759
5466
3898
7672
7449
8921
8014
1356
2350
3796
2500
1013
911208
14387
11911
12H7729-2times
CVJ-2-5-3-1-3
2218
2585
2767
2523
3674
4372
4483
4176
10053
9467
8664
9395
2417
3312
3345
3025
1372
813839
1314
713571
13H7729-2times1305
2169
2338
2547
2352
6728
6166
6792
6562
13333
11662
13301
1276
54128
4501
5660
4763
20061
17828
20093
19327
14H7729-2times(77371times
BSEC
TCP
-1)
1925
2095
2127
2049
3530
4165
4649
4115
9638
6917
7698
8084
1681
2833
3150
2555
1316
8110
82
12347
1219
915
H7729-2times96AC
-93
2012
2070
2180
2087
4955
4447
4596
4666
8738
6882
7762
7794
3754
3558
3181
3498
13693
11329
12359
12460
16H7729-2timesTo
go-II
2138
2428
2563
2377
4949
4660
5118
4909
8549
7243
7191
7661
3586
3353
3028
3322
13498
11903
12310
12570
17H7729-2times99HS-18
1899
1933
2073
1969
4046
4527
4988
4520
8492
7895
8144
8177
2561
3378
3553
3164
12539
12422
1313
212698
18G73-107times77245
1840
2253
2437
2177
2580
4776
5915
4424
11673
10608
10459
10913
1358
3163
4286
2936
14253
15385
16374
15337
19G73-107times
CVJ-2-5-3-1-3
2324
2537
2691
2517
4958
5030
5599
5196
9524
9395
1114
610022
3241
3645
4444
3776
14483
14425
1674
515217
20G73-107times1305
2228
2433
2583
2415
6340
5794
6373
6169
12490
1015
8119
61
11536
4199
4199
5018
4472
18830
1595
218334
1770
6
21G73-107times(77371times
BSEC
TCP
-1)
1943
2128
2220
2097
3936
3980
5770
4562
11697
9858
10303
10619
1874
2369
4181
2808
15633
13839
16073
1518
222
G73-107times96AC
-93
2118
2218
2317
2217
4270
5843
6201
5438
10319
9872
1015
910117
2703
4460
4379
3847
14589
15715
16359
15555
23G73-107timesTo
go-II
2237
2365
2733
2445
5736
5175
6822
5911
12098
10808
1299
611967
3984
3670
5372
4342
17835
1598
219818
17878
24G73-107times99HS-18
2025
2081
2322
2143
3558
4229
6612
4800
12350
9685
11516
1118
42196
2782
5009
3329
1590
813914
1812
915983
2577245timesCV
J-2-5-3-1-3
2109
2360
2675
2381
6760
6558
7299
6872
15225
11861
1416
21374
94225
4752
5839
4939
2198
518418
21461
20621
2677245times1305
1919
2100
2197
2072
5700
5142
5347
5397
11687
10565
10737
10996
4318
3956
4244
4173
17386
1570
816085
16393
2777245times(77371times
BSEC
TCP
-1)
1851
1935
2020
1936
2577
2460
2838
2625
6989
4778
4636
5468
1631
1662
1999
1764
9566
7238
7474
8093
2877245times96AC
-93
1729
1780
2133
1881
5541
5058
5748
5449
11952
8174
9526
9884
3646
3587
4071
3768
17493
13232
15274
15333
2977245timesTo
go-II
2300
2508
2658
2489
3067
5431
5963
4820
11737
11021
1216
411641
1598
4053
4770
3474
14805
16452
1812
616461
3077245times99HS-18
1982
2019
2223
2075
3846
3119
4225
3730
7493
6720
6476
6896
3205
2578
2643
2809
11339
9839
1070
110626
12 Advances in Agriculture
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
31CV
J-2-5-3-1-3times1305
2406
2553
3067
2675
4178
4796
6281
5085
13822
11425
12252
12500
2472
3241
4907
3540
18000
16222
18532
17585
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
2142
2173
2238
2184
4522
5235
5640
5132
11673
9658
10248
10526
2614
3766
4178
3519
1619
514893
15888
15659
33CV
J-2-5-3-1-3times
96AC
-93
2350
2477
2600
2476
4676
4280
6090
5015
11975
9832
10868
10891
3097
2931
4445
3491
16651
14111
1695
815907
34CV
J-2-5-3-1-3times
Togo-II
2093
2666
2989
2583
4058
3727
5190
4325
12588
10717
11217
11507
2742
2570
4185
3166
16645
14443
16407
15832
35CV
J-2-5-3-1-3times
99HS-18
2028
2311
2386
2242
3287
2513
5356
3719
10055
9733
1036
410051
2004
1581
4251
2612
13342
12246
1572
01376
9
361305times(77371times
BSEC
TCP
-1)
2005
2173
2210
2129
3569
4692
5140
4467
11652
9826
10041
10507
2005
3665
4179
3283
15221
14518
1518
214974
371305times96AC
-93
1822
2003
2047
1957
5115
4023
4813
4650
9965
7675
7571
8404
3456
2647
3155
3086
15080
11698
12384
13054
381305timesTo
go-II
2545
2583
2644
2591
6885
7283
6795
6987
12265
11410
12583
12086
4500
6120
5350
5323
1915
018693
19378
19074
391305times99HS-18
2070
2408
2593
2357
5219
4332
4800
4784
1092
97677
8968
9192
3924
3332
3840
3699
1614
912009
1376
913976
40(77371times
BSEC
TCP
-1)
times96AC
-93
2056
2115
2157
2109
3158
4463
4717
4113
9166
7540
8100
8268
1880
3542
3347
2923
12324
12003
12817
12381
41(77371times
BSEC
TCP
-1)
timesTo
go-II
1868
2253
2450
2190
4422
6458
7038
5973
1415
312369
1379
713439
2291
4646
5586
4174
18575
18827
20835
19412
42(77371times
BSEC
TCP
-1)
times99HS-18
1995
2026
2209
2077
2720
2909
3543
3057
7392
4692
5023
5702
1766
2187
2147
2033
10112
7601
8566
8760
4396AC
-93timesTo
go-II
1953
2027
2511
2164
5972
4391
7126
5830
15537
1392
714432
14632
3780
2538
5655
3991
21510
18318
21558
20462
4496AC
-93times99HS-18
1952
2021
2242
2072
3465
2758
4496
3573
5959
5186
6094
5746
2707
2154
2409
2423
9424
7944
10590
9319
45To
go-IItimes
99HS-18
1932
2008
2658
2199
5381
4995
5436
5270
10299
8876
9244
9473
3986
3842
3668
3832
15680
13871
14680
1474
3Mean
1982
2146
2301
2143
3963
4123
4836
4307
9498
8087
8663
8749
2510
2914
3513
2979
13460
12210
13499
13056
SE243
198
245
mdash671
501
626
mdash959
750
822
mdash423
349
344
mdash1155
927
1167
mdashCD
087
071
087
mdash239
178
223
mdash342
267
293
mdash15
112
412
2mdash
411
330
416
mdash
Advances in Agriculture 13Ta
ble4Analysis
ofvaria
ncefor
10times10
half-diallelfor
different
charactersin
threee
nviro
nments
(a)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Germination
()
Rateof
emergence
STI()
SSTI
()
Num
bero
fleavesseedling
Seedlin
gheight
(cm)
Freshweight
(g)seedlin
gDry
weight
(g)seedlin
g
E 1
Replicates
2652731
596768
783393
20700
93018674
33764
1033999
002590
Treatm
ents
541097
0255lowastlowast
8181153lowastlowast
30863092lowastlowast
20680900lowastlowast
1100708lowastlowast
2106317lowastlowast
930829527lowastlowast
319767lowastlowast
Parents
95118
163lowastlowast
14289925lowastlowast
59114816lowastlowast
18880254lowastlowast
1084227lowastlowast
1087906lowastlowast
22561719lowastlowast
042162lowastlowast
Hybrid
s44
11423573lowastlowast
3398933lowastlowast
25697199lowastlowast
20633716lowastlowast
472874lowastlowast
1391526lowastlowast
674123518lowastlowast
261839lowastlowast
Parentsv
ersush
ybrid
s1
43693104lowastlowast
163619
916lowastlowast
3896838
3896
2793lowastlowast
28873741lowastlowast
4272
2798lowastlowast
234144289lowastlowast
5367022lowastlowast
Error
108
281184
1356832
1173684
674337
040104
306
462
2015
1619
005535
E 2
Replicates
2583819
3707389lowast
1596635
198738
034925
448213
899529
007177
Treatm
ents
549997443lowastlowast
2825177lowastlowast
35378177lowastlowast
19074306lowastlowast
665284lowastlowast
1764
081lowastlowast
257694570lowastlowast
2177519lowastlowast
Parents
96507784lowastlowast
3718
879lowastlowast
69866289lowastlowast
1798
5404lowastlowast
705379lowastlowast
1408518lowastlowast
10612519lowastlowast
077429lowastlowast
Hybrid
s44
10569731lowastlowast
2127302lowastlowast
2910
2537lowastlowast
19429240lowastlowast
329256lowastlowast
7000
03lowastlowast
186026552lowastlowast
1483428lowastlowast
Parentsv
ersush
ybrid
s1
16223710lowastlowast
25488361lowastlowast
1113
326
13257362lowastlowast
15089673lowastlowast
5178
3569lowastlowast
5634825791lowastlowast
51618348lowastlowast
Error
108
334760
1097039
1049505
635570
06764
116
0576
694613
006206
E 3
Replicates
2894398
4808815lowast
mdashmdash
051076
095989
3768421
030220
Treatm
ents
541261560
0lowastlowast
4842216lowastlowast
mdashmdash
2811077lowastlowast
5947316lowastlowast
3870844
50lowastlowast
3445076lowastlowast
Parents
97135
735lowastlowast
1097
6388lowastlowast
mdashmdash
1468699lowastlowast
1112
764lowastlowast
136081650lowastlowast
1307836lowastlowast
Hybrid
s44
13217740lowastlowast
1379360
mdashmdash
15540
71lowastlowast
4581632lowastlowast
252084767lowastlowast
2378032lowastlowast
Parentsv
ersush
ybrid
s1
35440
180lowastlowast
102000335lowastlowast
mdashmdash
70200767lowastlowast
109548362lowastlowast
8586095705lowastlowast
69630188lowastlowast
Error
108
782907
1085444
mdashmdash
139771
291325
3019
169
02400
4(b)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Panicle
emergence
Effectiv
etillersplant
Planth
eight
Earlength
Earw
eightp
lant
Dry
fodd
eryieldplant
Grain
yieldplant
Totalbiological
yieldplant
E 1
Replicates
231860
61lowastlowast
416348lowastlowast
1672
9472lowast
1706
411077192
9634539
392777
115544
59Treatm
ents
544152
368lowastlowast
085657lowastlowast
326706089lowastlowast
1602572lowastlowast
6177
0406lowastlowast
304113
204lowastlowast
34285030lowastlowast
575617
582lowastlowast
Parents
94511481lowastlowast
018538lowastlowast
140807476lowastlowast
2240093lowastlowast
11653
959lowastlowast
27014155lowastlowast
3858650lowastlowast
61321919lowastlowast
Hybrid
s44
3201347lowastlowast
100797lowastlowast
110395657lowastlowast
1010
497lowastlowast
40296671lowastlowast
171271411lowastlowast
2413
6880lowastlowast
302999650lowastlowast
Parentsv
ersush
ybrid
s1
4276
5286lowastlowast
02360
9lowast11517452
616lowastlowast
2191
6167lowastlowast
1457662749lowastlowast
8643043524lowastlowast
7546
41053lowastlowast
17199467546lowastlowast
Error
108
468159
004907
3692099
224610
17114
303498126
682350
5075268
E 2
Replicates
2656364
002556
61575565lowastlowast
828842lowastlowast
589094
2268506
260877
1507116
Treatm
ents
542999618lowastlowast
069822lowastlowast
268998723lowastlowast
2436886lowastlowast
60592784lowastlowast
247259877lowastlowast
4192
0073lowastlowast
513483489lowastlowast
Parents
926300
00lowastlowast
048079lowastlowast
165555
405lowastlowast
3871874lowastlowast
1412
2393lowastlowast
19543682lowastlowast
3132
426lowastlowast
57230706lowastlowast
Hybrid
s44
2533
165lowastlowast
07344
9lowastlowast
9616
2857lowastlowast
1463359lowastlowast
35495366lowastlowast
154759288lowastlowast
26262465lowastlowast
293492304lowastlowast
Parentsv
ersush
ybrid
s1
26850135lowastlowast
105929lowastlowast
880476
6697lowastlowast
32357161lowastlowast
1583112746lowastlowast
6366731560lowastlowast
107994
3646lowastlowast
14299370699lowastlowast
Error
108
507598
005829
5234122
149406
955133
2142411
462311
3273886
E 3
Replicates
2029697
021161
9459847
143746
985141
46800
6492
6334
3345160
Treatm
ents
548045499lowastlowast
094511lowastlowast
192420108lowastlowast
3279225lowastlowast
72049211lowastlowast
298953
021lowastlowast
55061308lowastlowast
651732994lowastlowast
Parents
98010
741lowastlowast
094166lowastlowast
116037343lowastlowast
3702185lowastlowast
16305190lowastlowast
2323644
0lowastlowast
4553
893lowastlowast
71484982lowastlowast
Hybrid
s44
6254377lowastlowast
095514lowastlowast
7378
1513lowastlowast
2188348lowastlowast
31813193lowastlowast
190263916lowastlowast
31572179lowastlowast
368689934lowastlowast
Parentsv
ersush
ybrid
s1
8716
7710lowastlowast
053496lowast
609996
3199lowastlowast
47471212lowastlowast
2344
130203lowastlowast
756272
2861lowastlowast
154314
9707lowastlowast
18327859
719lowastlowast
Error
108
696981
007883
5578505
228608
1491014
2574
223
449588
5187705
lowast
Sign
ificant
at119875=005
lowastlowastSign
ificant
at119875=001
14 Advances in Agriculture
Table 5 Mean performance of the genotypes for membrane thermostability in two environments
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594
Advances in Agriculture 15
Table 5 Continued
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434
Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash
lowastValues are transformed values
0
6
12
18
24
30
36
42
48
54
minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity
G73-10796AC-93CVJ-2-5-3-1-3
Togo-II99HS-18
H77833-2H7729-2772451305
H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305
1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305
H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305
77245 times 1305
77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305
77371 times BSECT CP-1
H77833-2 times G73-107
Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits
moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling
survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The
16 Advances in Agriculture
Table6Po
oled
correla
tioncoeffi
cientamon
gvario
uscharactersin
threee
nviro
nments
Characters
Germination
Rateof
emergence
STI
SSTI
Leaves
seedlin
gSeedlin
gheight
Fresh
weight
Dry
weight
MTS
Panicle
emergence
Effectiv
etillersplant
Plant
height
Earlength
Ear
weightp
lant
Dry
fodd
eryield
plant
Grain
yield
plant
Total
biological
yield
plant
Germination
1000
0357lowastlowast
0353lowastlowast
0590lowastlowast
0230
0373lowastlowast
0293lowast
0260
0276lowastminus0135minus0478lowastlowast
0326lowast
minus0069minus0104
minus0190
minus0145
minus0167
Rateof
emergence
1000
0233
0351lowastlowast
0605lowastlowast
0585lowastlowast
0551lowastlowast
0524lowastlowast
0139minus0593lowastlowast
0032
0517lowastlowast
0217
0388lowastlowast
0361lowastlowast
0348lowastlowast
0377lowastlowast
STI
1000
0837lowastlowast
0241
0129
0081
004
80890lowastlowast
minus0215
minus0211
0030minus0007minus0043
minus0101
minus0111
minus0084
SSTI
1000
0233
0264
0183
0140
0745lowastlowast
minus0247minus0268lowast
0184minus0017minus006
6minus0129
minus0144
minus0112
Num
bero
fleaves
1000
0683lowastlowast
0633lowastlowast
060
4lowastlowast
0176minus0474lowastlowast
0109
0576lowastlowast
0496lowastlowast
064
1lowastlowast
0607lowastlowast
0630lowastlowast
0630lowastlowast
Seedlin
gheight
1000
0703lowastlowast
0663lowastlowast
0031minus0493lowastlowast
minus0115
0735lowastlowast
0360lowastlowast
0526lowastlowast
0520lowastlowast
0515lowastlowast
0532lowastlowast
Freshweight
1000
0957lowastlowast
minus0016
0425lowastlowast
minus0082
0661lowastlowast
0351lowastlowast
0581lowastlowast
0496lowastlowast
0576lowastlowast
0533lowastlowast
Dry
weight
1000minus0033minus0428lowastlowast
minus0016
0634lowastlowast
0354lowastlowast
0620lowastlowast
0509lowastlowast
0614lowastlowast
0554lowastlowast
MTS
1000minus040
minus0177minus0037
0015
minus0074
minus0138
minus0131
minus0120
Panicle
emergence
1000
minus0150minus0269lowast
0022minus0329lowast
minus0337lowast
minus0280lowast
minus0341lowast
Effectiv
etillersplant
1000
minus0012
0170
0366lowastlowast
040
5lowastlowast
0323lowast
0401lowastlowast
Planth
eight
1000
0650lowastlowast
0603lowastlowast
0596lowastlowast
0619lowastlowast
0610lowastlowast
Earlength
1000
0608lowastlowast
0671lowastlowast
064
8lowastlowast
066
4lowastlowast
Earw
eightp
lant
1000
0910lowastlowast
0984lowastlowast
0957lowastlowast
Dry
fodd
eryield
plant
1000
0907lowastlowast
0991lowastlowast
Grain
yield
plant
1000
0950lowastlowast
Totalbiological
yield
plant
1000
lowastSign
ificant
at119875=005lowastlowastSign
ificant
at119875=001
Advances in Agriculture 17
faster germination and early vigour in seedling could act asphysical indicators for heat tolerance
Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits
Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics
Conflict of Interests
The authors declare that there is no conflict of interestsregarding the publication of this paper
References
[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981
[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993
[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006
[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993
[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013
[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007
[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965
[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011
[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001
[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009
[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962
[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967
[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963
[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001
Submit your manuscripts athttpwwwhindawicom
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The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014
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Applied ampEnvironmentalSoil Science
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AgricultureAdvances in
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BiodiversityInternational Journal of
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GenomicsInternational Journal of
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Advances in Agriculture 5
the software SPSS (SPSS Inc) Multivariate clustering wasdone based on paired group cluster method using similaritycoefficient under Past 140 software [14] Multivariate analysisbased on the Euclidean distancematrix was estimated amonggenotypes to distinguish genotypes from each other Thegrouping of genotypes was generated using paired groupcluster method
3 Results and Discussion
The excessive heat (soil temperature up to 630∘C in E2)
about 18∘C higher than the normal atmospheric temperature(Figures 1(a) and 1(b)) was responsible for mortality ofseedlings Many seedlings were found desiccated at the pointof contact besides the desiccation at the tipsThe temperaturearound the tip level (5 cm above the soil surface) also reachedup to 50∘C (Figure 1(c)) It could therefore be concluded thatthe seedlings had to survive in a really high temperature Themoisture level remained above the permanent wilting point(Figure 1(d)) therefore drought effect was not in the playThe heat tolerance was measured as STI (Table 3) throughthe field screening technique of Peacock et al [4] Anotherindex known as SSTI was also calculated as an improvementand extensionThe analysis of variance (RBD) for 8 charactersin 3 environments showed significant genotypic differencesNo variability was observed for STI and SSTI in E
3only In
this environment no seedling mortality was observed andthe germination was taken as standard The values for STIand SSTI were therefore equal and the highest for all thegenotypes in this environment and hence no variation wasobserved
The hybrids were evaluated on the basis of mean perfor-mance for various characters in each environment (Table 3)On the mean basis STI value ranged from 5332 to 8224with an average of 7072 The hybrid H7729-2 times CVJ-2-5-3-1-3 was the best scorer for heat tolerance followed byH77833-2 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II The hybrid H77833-2 times 1305 was found susceptible toheat stress Seed to seedling thermotolerance index (SSTI)which is expected to be more rigorous index deviated thevalues from STI according to variation in germination undernormal environment (E
3) as the SSTI also coversincludes
the under surface mortality (USM) during germinationThe reductions in values were not strictly proportional tothe STI The reduction is dependent on thermosensitivityof germination We also came across such hybrids whichshowed poor germination and high STI SSTI values rangedfrom 4605 to 7287 with an average of 6090 The genotypeH77833-2 times 96AC-93 had the highest worth for SSTI onmean basis followed byG73-107times (77371timesBSECTCP-1) andH77833-2 times (77371 times BSECT CP-1) (Table 3) The analysisof variance for ten different characters in three differentenvironments (two stressed and one normal) was carried outand the results showed significant genotypic differences forall the characters (Table 4)
A caution is needed for evaluating such genotypes underSTI The SSTI gave a highly reduced value putting thesecrosses in the susceptible list It may be concluded therefore
that SSTI is more dependable and desirable tool for screeningand evaluating the genotypes for heat tolerance The germi-nation was lower in E
2than in E
1 This period has the highest
range of temperature with a high average Genotype-wise thehighest germination (6209) was recorded for the genotypeH7729-2 times (77371 times BSECT CP-1) on mean basis whichgives a preliminary indication of resistance to heat while thegenotype 1times 6 had the lowest germination (3533)The hybridH77833-2 times G73-107 was adjudged as the fastest emergenceof seedlingsThe genotype (77371timesBSECTCP-1)times 96AC-93had the highest fresh weightseedlingThe genotype H7729-2 times Togo-II had the highest seedling dry weight E
1had lower
dry weightseedling than E2indicating the more heat stress
during the E1
31 Membrane Thermostability Membrane thermostability(MTS) has long been recognised as a parameter of heattolerance All the hybrids were put to membrane thermosta-bility test in the laboratory under standard protocol Acompletely randomised design (CRD) analysis was carriedout for membrane thermostability (MTS) The mean sum ofsquares due to genotypes was highly significant indicatingthat enough genetic variability was present for membranethermostability (MTS) in each environment which justifiedfurther analysis of the data The range of values lay between5073 and 7742 Among the parents H77833-2 G73-107CVJ-2-5-3-1-3 77371 times BSECT CP-1 96AC-93 Togo-II and99HS-18 were verified as resistant genotypes and H7729-2 77245 and 1305 genotypes were found susceptible asmentioned in Table 1 (on the basis of previous experiment)Among all the hybrids H7729-2 times CVJ-2-5-3-1-3 CVJ-2-5-3-1-3 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II werethree highly tolerant genotypes on the basis of membranethermostability (Table 5)
32 Correlations The pooled correlations among the charac-ters were calculated (Table 6) around heat tolerance indices(STI SSTI and membrane thermostability) with a view tofind whether there was any association of these characterswith seedling as well as maturity traits It was observedthat STI and SSTI recorded the highest significant cor-relation among themselves as they are related indices forheat tolerance Membrane thermostability also showed highsignificant correlation with STI as well as SSTI STI hada significant positive association with germination of seedonly among seedling and maturity traits studied SSTI inaddition was also positively correlated with germination andemergence rate and negatively associated with number ofeffective tillersplantMembrane thermostability also showedsignificant positive correlation with seed germination amongseedling and maturity traits studied While evaluating thediallel progenies it was also seen that the heat toleranceindices STI SSTI and membrane thermostability were notshowing any perceptible correlation with the rest of thedevelopmental traits (seedling andmaturity traits)The stressperiod was terminated by irrigating the field experiment after15 days fromdate of sowingwhen the seedlings get establishedand further mortality stopped due to heat Moreover the soil
6 Advances in Agriculture
Table3Perfo
rmance
ofthep
arentsandthec
rosses
ford
ifferentcharacters
(a)
Sr
No
Genotypes
Germination(
)Ra
teof
emergencelowast
STIlowast
()
SSTIlowast(
)Num
bero
fleavesseedlin
gE 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
3941
3737
6164
4614
4037
4109
4279
4142
7830
7387
9000
8072
4498
4133
9000
5877
661
502
800
654
2H7729-2
5513
4726
6092
5443
2815
4980
3258
3684
4835
4495
9000
6110
4441
3602
9000
5681
363
294
427
361
3G73-107
5037
4826
5918
5260
4534
4132
4481
4382
6187
5413
9000
6866
5213
4460
9000
6224
878
710
935
841
477245
4747
4624
5565
4978
3625
4604
4047
4092
4385
3580
9000
5655
3803
3040
9000
5281
377
292
437
369
5CV
J-2-5-3-1-3
4824
4832
6897
5518
5044
4907
4960
4970
7193
6813
9000
7669
4959
4785
9000
6248
802
580
923
768
61305
4326
3891
5404
4540
3376
5056
3573
4002
3903
3281
9000
5395
3211
2452
9000
4888
350
284
414
349
777371timesBS
ECTCP
-14747
4502
6320
5190
4934
5075
5065
5025
6952
6706
9000
7552
5056
4671
9000
6242
621
542
700
621
896AC
-93
4651
3818
5722
4730
4312
4915
4753
4660
6660
6001
9000
7220
5212
3920
9000
6044
701
590
899
730
9To
go-II
4613
3937
5276
4609
4245
4737
4797
4593
7198
6861
9000
7686
5980
4767
9000
6582
681
629
853
721
1099HS-18
4575
3823
6247
4882
4317
4659
4746
4574
7594
7319
9000
7971
5154
4172
9000
6109
745
554
915
738
1H77833-2timesH7729-2
5787
5295
6733
5938
4964
5015
4989
4989
6803
6504
9000
7436
5808
5134
9000
6647
711
585
780
692
2H77833-2timesG73-107
5472
4417
6405
5431
5448
5422
5239
5370
7231
6769
9000
7667
5980
4556
9000
6512
1101
705
1340
1049
3H77833-2times77245
4076
3707
5504
4429
5217
5446
5238
5300
6627
6224
9000
7284
4690
4037
9000
5909
740
525
965
744
4H77833-2timesCV
J-2-5-3-1-3
4843
4300
5856
5000
4862
4956
4913
4911
7061
6519
9000
7527
5575
4632
9000
6403
998
885
1258
1047
5H77833-2times1305
3296
2780
4521
3533
5076
4907
5238
5074
3732
3265
9000
5332
2765
2050
9000
4605
1192
934
1600
1242
6H77833-2times(77371times
BSEC
TCP
-1)5636
5539
6258
5811
4489
5219
5015
4908
6981
6482
9000
7488
6146
5680
9000
6942
898
726
1525
1050
7H77833-2times96AC
-93
5720
5515
6335
5857
4800
5186
5075
5021
7833
7786
9000
8206
6603
6256
9000
7287
1107
873
1193
1058
8H77833-2timesTo
go-II
4862
3453
5709
4675
5036
4829
4911
4925
6190
5500
9000
6897
5214
3339
9000
5851
979
670
1107
919
9H77833-2times99HS-18
4575
4418
5447
4813
5041
5074
5006
5040
5800
5073
9000
6625
4846
4133
9000
5993
978
733
1135
949
10H7729-2timesG73-107
5615
4962
6273
5617
5023
5535
5165
5241
6143
5501
9000
6881
5540
4441
9000
6327
1000
720
1363
1028
11H7729-2times77245
5472
5164
6281
5639
4745
5031
5028
4935
5572
5175
9000
6582
4901
4345
9000
6082
880
620
897
799
12H7729-2timesCV
J-2-5-3-1-3
5494
5124
6536
5718
4804
5316
4957
5026
7596
8076
9000
8224
6093
5680
9000
6924
981
932
1451
1121
13H7729-2times1305
3804
3826
5511
4380
3596
4724
4387
4236
6362
7075
9000
7479
4230
4517
9000
5916
926
725
1497
1049
14H7729-2times(77371times
BSEC
TCP
-1)
6369
4847
7412
6209
4847
5351
5246
5148
6377
6031
9000
7136
5683
4230
9000
6304
977
671
1380
1009
15H7729-2times96AC
-93
5314
4657
6702
5558
4514
5295
5102
4971
5612
4856
9000
6489
4613
3603
9000
5739
846
679
1397
974
16H7729-2timesTo
go-II
5827
5594
6651
6024
5072
5279
5271
5207
6563
5371
9000
6978
5763
4652
9000
6472
873
719
1040
877
17H7729-2times99HS-18
5741
4638
7918
6099
4847
5024
5021
4964
6179
5603
9000
6927
4882
3725
9000
5869
1012
730
1600
1114
18G73-107times77245
5115
4646
6103
5288
4977
5242
5450
5223
7006
6063
9000
7356
5685
4594
9000
6426
1021
790
1292
1034
19G73-107timesCV
J-2-5-3-1-3
5134
4894
6427
5485
4901
5317
5181
5133
6637
6124
9000
7254
5253
4710
9000
6321
1039
863
1254
1052
20G73-107times1305
4881
4561
7597
5680
4717
4791
4775
4761
6704
6380
9000
7361
4556
4115
9000
5890
1140
882
1657
1226
21G73-107times(77371times
BSEC
TCP
-1)
5636
5519
6283
5812
4986
5219
5203
5136
7990
6203
9000
7731
6620
5436
9000
7019
977
703
1395
1025
22G73-107times96AC
-93
5740
4510
6415
5555
5129
4982
5195
5102
6978
5556
9000
7178
6156
4019
9000
6392
1077
860
1264
1067
23G73-107timesTo
go-II
4460
4357
6044
4953
4396
5138
4951
4829
5279
4835
9000
6371
4018
3603
9000
5540
906
881
1189
992
24G73-107times99HS-18
5473
4356
6374
5401
5102
4955
5055
5037
6460
5197
9000
6886
5534
3704
9000
6079
1171
766
1635
1191
2577245timesCV
J-2-5-3-1-3
4766
4666
5921
5117
5170
4918
4900
4996
5928
5859
9000
6929
4785
4613
9000
6133
984
920
1194
1033
2677245times1305
4575
4060
6052
4895
5128
5297
5203
5210
5102
4990
9000
6364
3979
3464
9000
5481
1279
823
1550
1217
2777245times(77371times
BSEC
TCP
-1)
5017
4929
6022
5322
5108
5473
5274
5285
5308
5044
9000
6451
4498
4191
9000
5896
833
588
939
787
Advances in Agriculture 7
(a)Con
tinued
Sr
No
Genotypes
Germination(
)Ra
teof
emergencelowast
STIlowast
()
SSTIlowast(
)Num
bero
fleavesseedlin
gE 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
2877245times96AC
-93
5472
4705
622154
66
5135
5197
5006
5113
5247
4843
9000
6363
4708
3784
9000
5831
795
662
1058
838
2977245timesTo
go-II
5252
4492
5889
5211
5188
4861
5074
5041
4885
4263
9000
6049
4421
3379
9000
5600
977
751
1243
990
3077245times99HS-18
5740
4099
6872
5570
5249
4898
5258
5135
5628
4616
9000
6415
4863
3014
9000
5626
1045
863
1184
1031
31CV
J-2-5-3-1-3times1305
4364
3944
5700
4669
4974
4517
4650
4714
6097
5579
9000
6892
4594
3842
9000
5812
936
848
1403
1063
32CV
J-2-5-3-1-3times(77371times
BSEC
TCP
-1)4345
3808
6774
4976
5272
4996
5035
5101
6650
5766
9000
7139
4288
3381
9000
5556
800
712
1013
841
33CV
J-2-5-3-1-3times96AC
-93
4862
4197
5743
4934
4884
4980
5058
4974
7477
6760
9000
7745
5957
4709
9000
6555
933
738
1478
1050
34CV
J-2-5-3-1-3timesTo
go-II
4670
4343
6233
5082
4399
4813
4631
4614
5806
6614
9000
7140
4422
4479
9000
5967
963
855
1180
999
35CV
J-2-5-3-1-3times99HS-18
5493
5080
7273
5949
5276
4984
5162
5141
7322
6590
9000
7637
5534
4766
9000
6433
998
807
1338
1047
361305times(77371times
BSEC
TCP
-1)
4613
4539
5717
4956
4986
4955
5080
5007
5137
4870
9000
6336
4210
3936
9000
5715
863
630
913
802
371305times96AC
-93
5473
5177
6830
5827
5224
4779
4919
4974
7117
6965
9000
7694
5640
5220
9000
6620
875
694
1476
1015
381305timesTo
go-II
4230
3420
5262
4304
4309
456144
45
4438
5781
4373
9000
6385
4575
2887
9000
5487
637
566
794
666
391305times99HS-18
5433
4507
6947
5629
5225
4753
4991
4989
7287
6968
9000
7752
5576
4479
9000
6352
1115
818
1410
1114
40(77371times
BSEC
TCP
-1)times
96AC
-93
5595
4429
7461
5828
5216
4955
5121
5097
7087
6368
9000
7485
5432
4038
9000
6157
955
753
1462
1057
41(77371times
BSEC
TCP
-1)times
Togo-II
5252
4728
6106
5362
5492
4808
5132
5144
8025
7505
9000
8177
6310
5413
9000
6908
859
693
990
848
42(77371times
BSEC
TCP
-1)times
99HS-18
5595
4298
6295
5396
4729
4887
5031
4883
7366
6945
9000
7770
6312
4556
9000
6623
903
622
1125
883
4396AC
-93timesTo
go-II
5017
3977
6486
5160
5019
5195
5237
5150
5922
5486
9000
6803
4689
3484
9000
5724
1007
728
1167
967
4496AC
-93times99HS-18
5595
4812
6924
5777
5036
5385
5205
5209
7411
5378
9000
7263
5873
3979
9000
6284
1013
652
1334
1000
45To
go-IItimes
99HS-18
4670
4497
7293
5487
4718
4319
4796
4611
5688
5431
9000
6706
3980
3665
9000
5548
939
653
1462
1018
Mean
5043
4482
6271
5265
4792
498149
23
4899
6377
584190
00
7072
5079
4190
9000
6090
898
701
1168
922
SE097
106
161
mdash213
191
190
mdash19
818
7mdash
mdash14
914
6mdash
mdash036
047
068
mdashCD
272
296
453
mdash597
537
534
mdash555
525
mdashmdash
421
409
mdashmdash
103
133
192
mdashlowastTransfo
rmed
data
(b)
Sr
No
Genotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
908
785
2245
1313
859
1537
1924
1440
154
143
160
152
6267
4800
3833
4967
212
227
249
229
15644
15458
13267
1479
02
H7729-2
1125
1175
1789
1363
317
2400
2811
1843
093
191
252
179
6700
5333
5100
5711
185
210
234
210
18850
18565
15389
17601
3G73-107
1166
1475
1966
1536
943
1744
4945
2544
143
129
366
213
6700
5433
5300
5811
218
219
238
225
15009
1496
513503
14492
477245
1438
1373
2085
1632
607
1863
5957
2809
117
180
509
269
6100
4800
5100
5333
232
221
253
235
14036
13802
11742
1319
35
CVJ-2-5-3-1-3
1214
1437
1807
1486
1163
1610
4166
2313
171
141
407
240
6300
4933
4600
5278
239
227
270
245
1994
317261
1497
017391
61305
858
1137
1737
1244
540
967
4861
2123
125
088
417
210
6533
5033
4800
5456
244
213
257
238
15527
15405
12274
14402
8 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
777371timesBS
ECT
CP-1
1377
1487
1892
1585
928
2660
3330
2306
150
258
301
236
5733
4533
4700
4989
242
220
293
252
1513
814550
1315
014279
896AC
-93
1303
1259
1852
1471
1056
1445
5972
2825
185
147
586
306
5500
4833
3833
4722
256
304
318
293
15577
14032
10000
13203
9To
go-II
1056
1220
2272
1516
924
1466
9113
3834
146
129
883
386
6167
4533
4600
5100
185
149
124
153
14578
12073
10033
12228
1099HS-18
1277
1075
1808
1387
1241
2833
7070
3715
228
226
615
356
6100
5000
4167
5089
243
173
324
247
12627
10355
1017
311052
1H77833-2times
H7729-2
1432
1498
2233
1721
1350
4405
8373
4709
159
499
782
480
5767
4433
3800
4667309
290
248
282
21010
18071
15847
18309
2H77833-2times
G73-107
1675
1635
2205
1838
1236
3211
5789
3412
134
313
547
331
5833
4767
3833
4811
262
295
236
265
21253
20449
15808
1917
0
3H77833-2times
77245
1577
1522
2263
1787
1058
6933
8719
5570
255
553
782
530
5267
4633
4600
4833
273
201
265
246
2074
119511
15208
18487
4H77833-2times
CVJ-2-5-3-1-3
1791
1757
2165
1904
20218030
5276
5109
354
779
477
537
5533
4233
3767
4511
165
330
351
282
21827
20072
17110
19670
5H77833-2times1305
1700
1842
28412128
1427
6228
9134
5596
570
579
813
654
5800
4267
3333
4467340
426
451
406
19816
19444
16037
18432
6H77833-2times
(77371times
BSEC
TCP
-1)
1453
1515
3120
2029
789
465510428
5291
211
408
858
492
5233
4167
3267
4222
203
283
267
251
20677
1913
816470
1876
2
7H77833-2times
96AC
-93
1835
1783
2427
2015
2532
6649
1610
684
29
350
650
1473
824
5467
4233
3700
4467243
239
256
246
22278
20434
15735
19482
8H77833-2times
Togo-II
1564
15632500
1876
1882
4772
11660
6105
296
469
972
579
5667
4533
440048
67
169
260
211
213
2412
82375
619389
22424
9H77833-2times
99HS-18
159318002049
1814
1571
480214024
6799
203
544
1547
765
6033
460040
0048
78
261
225
256
247
23466
21081
1793
320
827
10H7729-2times
G73-107
14951568
2075
1713
959
5157
8517
4877
218
382
666
422
6133
5067
4833
5344
300
273
239
271
23651
2191
717083
20884
11H7729-2times77245
1557
1537
2613
1902
1851
5756
5729
4445366
562
524
484
5633
4800
4633
5022
133
233
297
221
1979
219914
15250
18319
12H7729-2times
CVJ-2-5-3-1-3
18442033
28932257
1651
806114885
8199
264
597
1054
638
6333
4600
4233
5056
170
211
217
199
24629
23339
1970
022556
13H7729-2times1305
1325
1677309920
34
1756
724314048
7682
318
635
1232
728
630046
67
3633
4867298
261
361
307
21666
19371
17422
19486
14H7729-2times(77371
timesBS
ECTCP
-1)
1958
1887
3105
2317
1118
4675
14411
6734
242
457
1318
672
5900
4500
3800
4733
164
209
240
204
23661
1995
419250
20955
15H7729-2times
96AC
-93
1512
1717
2779
2002266844
08
11393
6156
356
500
1211
689
5633
4533
4200
4789
221
195
282
232
2194
921635
17213
20266
Advances in Agriculture 9
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
16H7729-2times
Togo-II
1519
1738
2159
1805
2437
9867
1616
294
89
361
961
1527
950
6167
4700
4200
5022
234
272
230
246
26338
2414
520553
23678
17H7729-2times
99HS-18
1938
1787
3151
2292
1574
6218
11318
6370
290
606
956
617
6067
4767
406749
67
172
210
199
194
24689
21854
1778
921444
18G73-107times77245
1856
1813
2210
1960
2435
8550
7828
6271338
633
612
528
5500
4633
440048
44
199
255
307
254
1993
619277
16095
18436
19G73-107times
CVJ-2-5-3-1-3
1517
1769
2407
1897
1556
7728
9136
6140
239
876
917
677
5900
4300
3400
4533
233
266
315
272
23418
22333
19317
21689
20G73-107times1305
1217
1507
2832
1852
1777
5189
1114
960
38
289
588
1197
691
6733
5000
4233
5322
286
210
312
269
2078
81992
817067
19261
21G73-107times(77371
timesBS
ECTCP
-1)
18551946
2730
2177
1801
3482
10415
5233
239
277
753
423
5500
4467
3433
4467243
197
273
238
23817
20263
17425
20502
22G73-107times
96AC
-93
1718
1840
28612140
1345
9333
14669
8449
297
845
1278
807
5600
5033
4633
5089
270
243
300
271
28537
21461
16875
22291
23G73-107timesTo
go-II
168018083087
2192
1112
4678
9319
5036
186
473
985
548
6033
4767
4200
5000
210
209
231
217
24864
24609
21625
23699
24G73-107times99HS-18
1398
1675
3370
2148
2753
8672
11918
7781
362
691
981
678
566746
00
3500
4589
170
247
270
229
24677
20567
17855
21033
2577245times
CVJ-2-5-3-1-3
1500
1728
2203
1810
2282
4679
11492
6151
328
424
1116
623
6000
4333
420048
44300
231
370
300
21257
20497
1772
519826
2677245times1305
143815772080
1698
949
10439
12053
7814
121
915
992
676
5767
4800
3500
4689
168246
316
244
1978
91779
015517
17699
2777245times(77371times
BSEC
TCP
-1)
150516972846
2016
3222
8518
12621
8120
469
827
1141
812
5400
4367
3833
4533
196
225
242
221
2092
819485
1719
219202
2877245times96AC
-93
1653
1797
2117
1856
1934
6056
11543
6511
352
563
911
608
5333
4267
3833
4478
186
169
198
184
22003
19054
1676
719275
2977245timesTo
go-II
1723
1718
3163
2201
1749
8250
15443
8481229
806
1412
816
5967
4700
4067
4911
174
245
298
239
22075
23503
19875
21818
3077245times99HS-18
2016
1970
28542280
3217
13027
3531
6591395
965
285
549
5733
4467
3833
4678
148
228
209
195
2212
721462
1690
020
163
31CV
J-2-5-3-1-3times
1305
1358
1422
2179
1653
1254
2803
1019
44750
178
254
801
411
6033
4467
3367
4622
312
193
330
278
22269
19547
17250
19689
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
1335
1742
2400
1826
922
5226
12806
6318
236
458
1054
583
5633
4433
3600
4556
321
315
259
298
2210
419524
17087
19572
33CV
J-2-5-3-1-3times
96AC
-93
1552
16803260
2164
166710094
11941
7901250
974
1115
779
5533
4533
446748
44
170
202
317
230
2392
123317
19333
2219
0
34CV
J-2-5-3-1-3times
Togo-II
142216072638
1889
1012
4163
11061
5412
261
377
891
510
6167
4967
4533
5222
207
225
325
252
23590
22860
20278
22243
35CV
J-2-5-3-1-3times
99HS-18
1435
1600
2700
1912
3056
3237
7089
4461449
301
658
469
5700
44673800
4656
148
178
225
183
22444
20240
1696
719884
361305times(77371times
BSEC
TCP
-1)
1499
1767
2058
1774
1882
4665
7920
4822
356
426
724
502
566742673800
4578
271
242
305
273
22011
19295
16417
19241
371305times96AC
-93
16781605
2757
2014
1516
10037
11960
7838
311
11381284
911
560040
673400
4356
182
276
251
236
2396
519625
15800
1979
738
1305timesTo
go-II
1177
16802249
1702
902
4676
9388
4989
241
456
795
497
6533
5533
4733
5600
214
266
296
259
21894
20217
19222
20444
391305times99HS-18
1774
1767
2766
2102
2145
4628
10618
5797
301
447
991
580
5933
4500
3733
4722
128
142
180
150
2275
719413
16967
1971
2
40(77371times
BSEC
TCP
-1)times
96AC
-93
1748
1795
31712238
1537
12586
14379
9501
211
1104
1183
833
546744
00346744
44
127
201
226
185
23294
21089
17858
2074
7
10 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(
days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
41(77371times
BSEC
TCP
-1)times
Togo-II
19652007
2653
2208
2136
5601
9503
5747
315
635
1001
650
5533
4833
410048
22
213
241
286
247
22662
22094
17667
20808
42(77371times
BSEC
TCP
-1)times
99HS-18
15011687
2705
1964
1935
7253
11806
6998
314
704
1067
695
5467
4433
5133
5011
177
167
175
173
22305
19277
16392
19325
4396AC
-93timesTo
go-II1759
1618
2123
1834
1539
10347
12212
8033
325
907
1090
774
5900
450039674789
214
269
339
274
23252
21554
16875
20560
4496AC
-93times
99HS-18
1353
1618
2925
1966
3532
7223
11950
7569
440
654
1024
706
5600
4900
4533
5011
149
175
206
177
24494
20698
18111
2110
145
Togo-IItimes
99HS-18
1117
1278
2582
1659
3448
6764
11910
7374
477
767
1207
817
6000
5133
4333
5156
183
203
216
201
17683
15541
14333
15853
Mean
1513
1618
2492
1874
1656
5773
9854
5761
272
538
885
565
586846
534116
4879
218
233
268
240
21297
19547
16529
1912
4SE
101
073
098
mdash079
152
317
mdash014
014
028
mdash351
365
428
mdash036
039
046
mdash985
1173
1211
mdashCD
284
205
276
mdash221
427
890
mdash038
040
079
mdash12
513
015
2mdash
013
014
016
mdash351
418
431
mdash
(c)
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
1642
1667
1744
1684
1504
1349
1526
1460
2783
2405
2634
2607
836
745
908
830
4287
3754
4160
4067
2H7729-2
1693
1817
1950
1820
1012
859
1016
962
3433
2809
2591
2944
649
597
651
632
4445
3668
3607
3906
3G73-107
2000
2028
2077
2035
2280
1966
2312
2186
4695
4023
4229
4316
1281
1170
1463
1305
6975
5989
6541
6502
477245
1523
1630
1728
1627
1796
2489
2702
2329
5423
4851
4498
4924
748
1464
1743
1319
7219
7341
7200
7253
5CV
J-2-5-3-1-3
2282
2692
2787
2587
2411
2643
2901
2652
5857
4836
5024
5239
1206
1335
1769
1437
8268
7479
7925
7891
61305
1851
1951
2054
1952
2026
1763
1908
1899
4657
4414
4463
4511
1468
1336
1539
1448
6683
6177
6371
6410
777371timesBS
ECTCP
-11629
1645
1733
1669
1244
1692
1884
1607
4925
4183
4435
4515
625
1092
1483
1067
6169
5875
6319
6121
896AC
-93
1379
1519
1600
1499
2066
2942
3088
2698
4501
4095
4229
4275
984
1565
1678
1409
6567
7037
7316
6973
9To
go-II
1904
2057
2103
2021
2167
1844
2358
2123
4520
3446
3904
3957
1298
1138
1502
1313
6687
5290
6262
6080
1099HS-18
1475
1485
1633
1531
3180
2904
3382
3155
5633
4139
5205
4992
1656
1536
1879
1690
8813
7043
8587
8147
1H77833-2timesH7729-2
2010
2046
2133
2063
3645
3075
4377
3699
7675
6540
7199
7138
2014
1708
2880
2201
11320
9615
11576
10837
2H77833-2timesG73-107
2066
2101
2122
2096
5002
4053
4601
4552
7882
7430
6963
7425
3146
2565
2808
2840
12884
11482
11564
11977
3H77833-2times77245
1764
2048
2083
1965
3769
4497
4559
4275
8681
8515
8475
8557
2284
3356
3377
3006
12450
13012
13034
12832
4H77833-2times
CVJ-2-5-3-1-3
2205
2383
2733
2441
3693
5655
5220
4856
11656
10287
1010
510683
2198
4488
4176
3621
15348
1594
215325
15538
5H77833-2times1305
2209
2423
2491
2374
6008
5842
6933
6261
15238
14311
13889
14479
3758
4057
5591
4469
21246
2015
320822
2074
0
6H77833-2times(77371times
BSEC
TCP
-1)
1789
1823
1992
1868
3593
4288
4903
4262
8922
6522
7429
7625
2178
3224
3070
2824
12515
10811
12333
11886
7H77833-2times96AC
-93
1739
1960
2011
1903
4557
4350
4818
4575
7916
6068
7290
7092
2411
2529
3191
2710
12474
10419
1210
811667
8H77833-2timesTo
go-II
1927
2389
2406
2241
4185
5828
6215
5409
12319
10279
11023
11207
2433
4254
4631
3773
16504
1610
717238
16616
9H77833-2times99HS-18
1941
2245
2330
2172
4227
4139
4529
4298
9211
8120
7969
8433
3154
3184
3253
3197
13438
12259
12498
1273
210
H7729-2timesG73-107
1968
2109
2158
2079
3725
3042
3488
3418
7313
5956
6174
6481
2822
2001
2357
2393
11038
8998
9662
9899
Advances in Agriculture 11
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
11H7729-2times77245
1847
2048
2200
2032
2467
3759
5466
3898
7672
7449
8921
8014
1356
2350
3796
2500
1013
911208
14387
11911
12H7729-2times
CVJ-2-5-3-1-3
2218
2585
2767
2523
3674
4372
4483
4176
10053
9467
8664
9395
2417
3312
3345
3025
1372
813839
1314
713571
13H7729-2times1305
2169
2338
2547
2352
6728
6166
6792
6562
13333
11662
13301
1276
54128
4501
5660
4763
20061
17828
20093
19327
14H7729-2times(77371times
BSEC
TCP
-1)
1925
2095
2127
2049
3530
4165
4649
4115
9638
6917
7698
8084
1681
2833
3150
2555
1316
8110
82
12347
1219
915
H7729-2times96AC
-93
2012
2070
2180
2087
4955
4447
4596
4666
8738
6882
7762
7794
3754
3558
3181
3498
13693
11329
12359
12460
16H7729-2timesTo
go-II
2138
2428
2563
2377
4949
4660
5118
4909
8549
7243
7191
7661
3586
3353
3028
3322
13498
11903
12310
12570
17H7729-2times99HS-18
1899
1933
2073
1969
4046
4527
4988
4520
8492
7895
8144
8177
2561
3378
3553
3164
12539
12422
1313
212698
18G73-107times77245
1840
2253
2437
2177
2580
4776
5915
4424
11673
10608
10459
10913
1358
3163
4286
2936
14253
15385
16374
15337
19G73-107times
CVJ-2-5-3-1-3
2324
2537
2691
2517
4958
5030
5599
5196
9524
9395
1114
610022
3241
3645
4444
3776
14483
14425
1674
515217
20G73-107times1305
2228
2433
2583
2415
6340
5794
6373
6169
12490
1015
8119
61
11536
4199
4199
5018
4472
18830
1595
218334
1770
6
21G73-107times(77371times
BSEC
TCP
-1)
1943
2128
2220
2097
3936
3980
5770
4562
11697
9858
10303
10619
1874
2369
4181
2808
15633
13839
16073
1518
222
G73-107times96AC
-93
2118
2218
2317
2217
4270
5843
6201
5438
10319
9872
1015
910117
2703
4460
4379
3847
14589
15715
16359
15555
23G73-107timesTo
go-II
2237
2365
2733
2445
5736
5175
6822
5911
12098
10808
1299
611967
3984
3670
5372
4342
17835
1598
219818
17878
24G73-107times99HS-18
2025
2081
2322
2143
3558
4229
6612
4800
12350
9685
11516
1118
42196
2782
5009
3329
1590
813914
1812
915983
2577245timesCV
J-2-5-3-1-3
2109
2360
2675
2381
6760
6558
7299
6872
15225
11861
1416
21374
94225
4752
5839
4939
2198
518418
21461
20621
2677245times1305
1919
2100
2197
2072
5700
5142
5347
5397
11687
10565
10737
10996
4318
3956
4244
4173
17386
1570
816085
16393
2777245times(77371times
BSEC
TCP
-1)
1851
1935
2020
1936
2577
2460
2838
2625
6989
4778
4636
5468
1631
1662
1999
1764
9566
7238
7474
8093
2877245times96AC
-93
1729
1780
2133
1881
5541
5058
5748
5449
11952
8174
9526
9884
3646
3587
4071
3768
17493
13232
15274
15333
2977245timesTo
go-II
2300
2508
2658
2489
3067
5431
5963
4820
11737
11021
1216
411641
1598
4053
4770
3474
14805
16452
1812
616461
3077245times99HS-18
1982
2019
2223
2075
3846
3119
4225
3730
7493
6720
6476
6896
3205
2578
2643
2809
11339
9839
1070
110626
12 Advances in Agriculture
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
31CV
J-2-5-3-1-3times1305
2406
2553
3067
2675
4178
4796
6281
5085
13822
11425
12252
12500
2472
3241
4907
3540
18000
16222
18532
17585
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
2142
2173
2238
2184
4522
5235
5640
5132
11673
9658
10248
10526
2614
3766
4178
3519
1619
514893
15888
15659
33CV
J-2-5-3-1-3times
96AC
-93
2350
2477
2600
2476
4676
4280
6090
5015
11975
9832
10868
10891
3097
2931
4445
3491
16651
14111
1695
815907
34CV
J-2-5-3-1-3times
Togo-II
2093
2666
2989
2583
4058
3727
5190
4325
12588
10717
11217
11507
2742
2570
4185
3166
16645
14443
16407
15832
35CV
J-2-5-3-1-3times
99HS-18
2028
2311
2386
2242
3287
2513
5356
3719
10055
9733
1036
410051
2004
1581
4251
2612
13342
12246
1572
01376
9
361305times(77371times
BSEC
TCP
-1)
2005
2173
2210
2129
3569
4692
5140
4467
11652
9826
10041
10507
2005
3665
4179
3283
15221
14518
1518
214974
371305times96AC
-93
1822
2003
2047
1957
5115
4023
4813
4650
9965
7675
7571
8404
3456
2647
3155
3086
15080
11698
12384
13054
381305timesTo
go-II
2545
2583
2644
2591
6885
7283
6795
6987
12265
11410
12583
12086
4500
6120
5350
5323
1915
018693
19378
19074
391305times99HS-18
2070
2408
2593
2357
5219
4332
4800
4784
1092
97677
8968
9192
3924
3332
3840
3699
1614
912009
1376
913976
40(77371times
BSEC
TCP
-1)
times96AC
-93
2056
2115
2157
2109
3158
4463
4717
4113
9166
7540
8100
8268
1880
3542
3347
2923
12324
12003
12817
12381
41(77371times
BSEC
TCP
-1)
timesTo
go-II
1868
2253
2450
2190
4422
6458
7038
5973
1415
312369
1379
713439
2291
4646
5586
4174
18575
18827
20835
19412
42(77371times
BSEC
TCP
-1)
times99HS-18
1995
2026
2209
2077
2720
2909
3543
3057
7392
4692
5023
5702
1766
2187
2147
2033
10112
7601
8566
8760
4396AC
-93timesTo
go-II
1953
2027
2511
2164
5972
4391
7126
5830
15537
1392
714432
14632
3780
2538
5655
3991
21510
18318
21558
20462
4496AC
-93times99HS-18
1952
2021
2242
2072
3465
2758
4496
3573
5959
5186
6094
5746
2707
2154
2409
2423
9424
7944
10590
9319
45To
go-IItimes
99HS-18
1932
2008
2658
2199
5381
4995
5436
5270
10299
8876
9244
9473
3986
3842
3668
3832
15680
13871
14680
1474
3Mean
1982
2146
2301
2143
3963
4123
4836
4307
9498
8087
8663
8749
2510
2914
3513
2979
13460
12210
13499
13056
SE243
198
245
mdash671
501
626
mdash959
750
822
mdash423
349
344
mdash1155
927
1167
mdashCD
087
071
087
mdash239
178
223
mdash342
267
293
mdash15
112
412
2mdash
411
330
416
mdash
Advances in Agriculture 13Ta
ble4Analysis
ofvaria
ncefor
10times10
half-diallelfor
different
charactersin
threee
nviro
nments
(a)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Germination
()
Rateof
emergence
STI()
SSTI
()
Num
bero
fleavesseedling
Seedlin
gheight
(cm)
Freshweight
(g)seedlin
gDry
weight
(g)seedlin
g
E 1
Replicates
2652731
596768
783393
20700
93018674
33764
1033999
002590
Treatm
ents
541097
0255lowastlowast
8181153lowastlowast
30863092lowastlowast
20680900lowastlowast
1100708lowastlowast
2106317lowastlowast
930829527lowastlowast
319767lowastlowast
Parents
95118
163lowastlowast
14289925lowastlowast
59114816lowastlowast
18880254lowastlowast
1084227lowastlowast
1087906lowastlowast
22561719lowastlowast
042162lowastlowast
Hybrid
s44
11423573lowastlowast
3398933lowastlowast
25697199lowastlowast
20633716lowastlowast
472874lowastlowast
1391526lowastlowast
674123518lowastlowast
261839lowastlowast
Parentsv
ersush
ybrid
s1
43693104lowastlowast
163619
916lowastlowast
3896838
3896
2793lowastlowast
28873741lowastlowast
4272
2798lowastlowast
234144289lowastlowast
5367022lowastlowast
Error
108
281184
1356832
1173684
674337
040104
306
462
2015
1619
005535
E 2
Replicates
2583819
3707389lowast
1596635
198738
034925
448213
899529
007177
Treatm
ents
549997443lowastlowast
2825177lowastlowast
35378177lowastlowast
19074306lowastlowast
665284lowastlowast
1764
081lowastlowast
257694570lowastlowast
2177519lowastlowast
Parents
96507784lowastlowast
3718
879lowastlowast
69866289lowastlowast
1798
5404lowastlowast
705379lowastlowast
1408518lowastlowast
10612519lowastlowast
077429lowastlowast
Hybrid
s44
10569731lowastlowast
2127302lowastlowast
2910
2537lowastlowast
19429240lowastlowast
329256lowastlowast
7000
03lowastlowast
186026552lowastlowast
1483428lowastlowast
Parentsv
ersush
ybrid
s1
16223710lowastlowast
25488361lowastlowast
1113
326
13257362lowastlowast
15089673lowastlowast
5178
3569lowastlowast
5634825791lowastlowast
51618348lowastlowast
Error
108
334760
1097039
1049505
635570
06764
116
0576
694613
006206
E 3
Replicates
2894398
4808815lowast
mdashmdash
051076
095989
3768421
030220
Treatm
ents
541261560
0lowastlowast
4842216lowastlowast
mdashmdash
2811077lowastlowast
5947316lowastlowast
3870844
50lowastlowast
3445076lowastlowast
Parents
97135
735lowastlowast
1097
6388lowastlowast
mdashmdash
1468699lowastlowast
1112
764lowastlowast
136081650lowastlowast
1307836lowastlowast
Hybrid
s44
13217740lowastlowast
1379360
mdashmdash
15540
71lowastlowast
4581632lowastlowast
252084767lowastlowast
2378032lowastlowast
Parentsv
ersush
ybrid
s1
35440
180lowastlowast
102000335lowastlowast
mdashmdash
70200767lowastlowast
109548362lowastlowast
8586095705lowastlowast
69630188lowastlowast
Error
108
782907
1085444
mdashmdash
139771
291325
3019
169
02400
4(b)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Panicle
emergence
Effectiv
etillersplant
Planth
eight
Earlength
Earw
eightp
lant
Dry
fodd
eryieldplant
Grain
yieldplant
Totalbiological
yieldplant
E 1
Replicates
231860
61lowastlowast
416348lowastlowast
1672
9472lowast
1706
411077192
9634539
392777
115544
59Treatm
ents
544152
368lowastlowast
085657lowastlowast
326706089lowastlowast
1602572lowastlowast
6177
0406lowastlowast
304113
204lowastlowast
34285030lowastlowast
575617
582lowastlowast
Parents
94511481lowastlowast
018538lowastlowast
140807476lowastlowast
2240093lowastlowast
11653
959lowastlowast
27014155lowastlowast
3858650lowastlowast
61321919lowastlowast
Hybrid
s44
3201347lowastlowast
100797lowastlowast
110395657lowastlowast
1010
497lowastlowast
40296671lowastlowast
171271411lowastlowast
2413
6880lowastlowast
302999650lowastlowast
Parentsv
ersush
ybrid
s1
4276
5286lowastlowast
02360
9lowast11517452
616lowastlowast
2191
6167lowastlowast
1457662749lowastlowast
8643043524lowastlowast
7546
41053lowastlowast
17199467546lowastlowast
Error
108
468159
004907
3692099
224610
17114
303498126
682350
5075268
E 2
Replicates
2656364
002556
61575565lowastlowast
828842lowastlowast
589094
2268506
260877
1507116
Treatm
ents
542999618lowastlowast
069822lowastlowast
268998723lowastlowast
2436886lowastlowast
60592784lowastlowast
247259877lowastlowast
4192
0073lowastlowast
513483489lowastlowast
Parents
926300
00lowastlowast
048079lowastlowast
165555
405lowastlowast
3871874lowastlowast
1412
2393lowastlowast
19543682lowastlowast
3132
426lowastlowast
57230706lowastlowast
Hybrid
s44
2533
165lowastlowast
07344
9lowastlowast
9616
2857lowastlowast
1463359lowastlowast
35495366lowastlowast
154759288lowastlowast
26262465lowastlowast
293492304lowastlowast
Parentsv
ersush
ybrid
s1
26850135lowastlowast
105929lowastlowast
880476
6697lowastlowast
32357161lowastlowast
1583112746lowastlowast
6366731560lowastlowast
107994
3646lowastlowast
14299370699lowastlowast
Error
108
507598
005829
5234122
149406
955133
2142411
462311
3273886
E 3
Replicates
2029697
021161
9459847
143746
985141
46800
6492
6334
3345160
Treatm
ents
548045499lowastlowast
094511lowastlowast
192420108lowastlowast
3279225lowastlowast
72049211lowastlowast
298953
021lowastlowast
55061308lowastlowast
651732994lowastlowast
Parents
98010
741lowastlowast
094166lowastlowast
116037343lowastlowast
3702185lowastlowast
16305190lowastlowast
2323644
0lowastlowast
4553
893lowastlowast
71484982lowastlowast
Hybrid
s44
6254377lowastlowast
095514lowastlowast
7378
1513lowastlowast
2188348lowastlowast
31813193lowastlowast
190263916lowastlowast
31572179lowastlowast
368689934lowastlowast
Parentsv
ersush
ybrid
s1
8716
7710lowastlowast
053496lowast
609996
3199lowastlowast
47471212lowastlowast
2344
130203lowastlowast
756272
2861lowastlowast
154314
9707lowastlowast
18327859
719lowastlowast
Error
108
696981
007883
5578505
228608
1491014
2574
223
449588
5187705
lowast
Sign
ificant
at119875=005
lowastlowastSign
ificant
at119875=001
14 Advances in Agriculture
Table 5 Mean performance of the genotypes for membrane thermostability in two environments
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594
Advances in Agriculture 15
Table 5 Continued
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434
Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash
lowastValues are transformed values
0
6
12
18
24
30
36
42
48
54
minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity
G73-10796AC-93CVJ-2-5-3-1-3
Togo-II99HS-18
H77833-2H7729-2772451305
H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305
1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305
H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305
77245 times 1305
77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305
77371 times BSECT CP-1
H77833-2 times G73-107
Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits
moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling
survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The
16 Advances in Agriculture
Table6Po
oled
correla
tioncoeffi
cientamon
gvario
uscharactersin
threee
nviro
nments
Characters
Germination
Rateof
emergence
STI
SSTI
Leaves
seedlin
gSeedlin
gheight
Fresh
weight
Dry
weight
MTS
Panicle
emergence
Effectiv
etillersplant
Plant
height
Earlength
Ear
weightp
lant
Dry
fodd
eryield
plant
Grain
yield
plant
Total
biological
yield
plant
Germination
1000
0357lowastlowast
0353lowastlowast
0590lowastlowast
0230
0373lowastlowast
0293lowast
0260
0276lowastminus0135minus0478lowastlowast
0326lowast
minus0069minus0104
minus0190
minus0145
minus0167
Rateof
emergence
1000
0233
0351lowastlowast
0605lowastlowast
0585lowastlowast
0551lowastlowast
0524lowastlowast
0139minus0593lowastlowast
0032
0517lowastlowast
0217
0388lowastlowast
0361lowastlowast
0348lowastlowast
0377lowastlowast
STI
1000
0837lowastlowast
0241
0129
0081
004
80890lowastlowast
minus0215
minus0211
0030minus0007minus0043
minus0101
minus0111
minus0084
SSTI
1000
0233
0264
0183
0140
0745lowastlowast
minus0247minus0268lowast
0184minus0017minus006
6minus0129
minus0144
minus0112
Num
bero
fleaves
1000
0683lowastlowast
0633lowastlowast
060
4lowastlowast
0176minus0474lowastlowast
0109
0576lowastlowast
0496lowastlowast
064
1lowastlowast
0607lowastlowast
0630lowastlowast
0630lowastlowast
Seedlin
gheight
1000
0703lowastlowast
0663lowastlowast
0031minus0493lowastlowast
minus0115
0735lowastlowast
0360lowastlowast
0526lowastlowast
0520lowastlowast
0515lowastlowast
0532lowastlowast
Freshweight
1000
0957lowastlowast
minus0016
0425lowastlowast
minus0082
0661lowastlowast
0351lowastlowast
0581lowastlowast
0496lowastlowast
0576lowastlowast
0533lowastlowast
Dry
weight
1000minus0033minus0428lowastlowast
minus0016
0634lowastlowast
0354lowastlowast
0620lowastlowast
0509lowastlowast
0614lowastlowast
0554lowastlowast
MTS
1000minus040
minus0177minus0037
0015
minus0074
minus0138
minus0131
minus0120
Panicle
emergence
1000
minus0150minus0269lowast
0022minus0329lowast
minus0337lowast
minus0280lowast
minus0341lowast
Effectiv
etillersplant
1000
minus0012
0170
0366lowastlowast
040
5lowastlowast
0323lowast
0401lowastlowast
Planth
eight
1000
0650lowastlowast
0603lowastlowast
0596lowastlowast
0619lowastlowast
0610lowastlowast
Earlength
1000
0608lowastlowast
0671lowastlowast
064
8lowastlowast
066
4lowastlowast
Earw
eightp
lant
1000
0910lowastlowast
0984lowastlowast
0957lowastlowast
Dry
fodd
eryield
plant
1000
0907lowastlowast
0991lowastlowast
Grain
yield
plant
1000
0950lowastlowast
Totalbiological
yield
plant
1000
lowastSign
ificant
at119875=005lowastlowastSign
ificant
at119875=001
Advances in Agriculture 17
faster germination and early vigour in seedling could act asphysical indicators for heat tolerance
Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits
Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics
Conflict of Interests
The authors declare that there is no conflict of interestsregarding the publication of this paper
References
[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981
[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993
[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006
[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993
[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013
[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007
[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965
[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011
[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001
[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009
[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962
[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967
[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963
[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001
Submit your manuscripts athttpwwwhindawicom
Nutrition and Metabolism
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Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Food ScienceInternational Journal of
Agronomy
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
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International Journal of
Microbiology
The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom
Applied ampEnvironmentalSoil Science
Volume 2014
AgricultureAdvances in
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
BiodiversityInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
GenomicsInternational Journal of
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Plant GenomicsInternational Journal of
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Biotechnology Research International
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Forestry ResearchInternational Journal of
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Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
EcologyInternational Journal of
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Cell BiologyInternational Journal of
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Evolutionary BiologyInternational Journal of
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6 Advances in Agriculture
Table3Perfo
rmance
ofthep
arentsandthec
rosses
ford
ifferentcharacters
(a)
Sr
No
Genotypes
Germination(
)Ra
teof
emergencelowast
STIlowast
()
SSTIlowast(
)Num
bero
fleavesseedlin
gE 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
3941
3737
6164
4614
4037
4109
4279
4142
7830
7387
9000
8072
4498
4133
9000
5877
661
502
800
654
2H7729-2
5513
4726
6092
5443
2815
4980
3258
3684
4835
4495
9000
6110
4441
3602
9000
5681
363
294
427
361
3G73-107
5037
4826
5918
5260
4534
4132
4481
4382
6187
5413
9000
6866
5213
4460
9000
6224
878
710
935
841
477245
4747
4624
5565
4978
3625
4604
4047
4092
4385
3580
9000
5655
3803
3040
9000
5281
377
292
437
369
5CV
J-2-5-3-1-3
4824
4832
6897
5518
5044
4907
4960
4970
7193
6813
9000
7669
4959
4785
9000
6248
802
580
923
768
61305
4326
3891
5404
4540
3376
5056
3573
4002
3903
3281
9000
5395
3211
2452
9000
4888
350
284
414
349
777371timesBS
ECTCP
-14747
4502
6320
5190
4934
5075
5065
5025
6952
6706
9000
7552
5056
4671
9000
6242
621
542
700
621
896AC
-93
4651
3818
5722
4730
4312
4915
4753
4660
6660
6001
9000
7220
5212
3920
9000
6044
701
590
899
730
9To
go-II
4613
3937
5276
4609
4245
4737
4797
4593
7198
6861
9000
7686
5980
4767
9000
6582
681
629
853
721
1099HS-18
4575
3823
6247
4882
4317
4659
4746
4574
7594
7319
9000
7971
5154
4172
9000
6109
745
554
915
738
1H77833-2timesH7729-2
5787
5295
6733
5938
4964
5015
4989
4989
6803
6504
9000
7436
5808
5134
9000
6647
711
585
780
692
2H77833-2timesG73-107
5472
4417
6405
5431
5448
5422
5239
5370
7231
6769
9000
7667
5980
4556
9000
6512
1101
705
1340
1049
3H77833-2times77245
4076
3707
5504
4429
5217
5446
5238
5300
6627
6224
9000
7284
4690
4037
9000
5909
740
525
965
744
4H77833-2timesCV
J-2-5-3-1-3
4843
4300
5856
5000
4862
4956
4913
4911
7061
6519
9000
7527
5575
4632
9000
6403
998
885
1258
1047
5H77833-2times1305
3296
2780
4521
3533
5076
4907
5238
5074
3732
3265
9000
5332
2765
2050
9000
4605
1192
934
1600
1242
6H77833-2times(77371times
BSEC
TCP
-1)5636
5539
6258
5811
4489
5219
5015
4908
6981
6482
9000
7488
6146
5680
9000
6942
898
726
1525
1050
7H77833-2times96AC
-93
5720
5515
6335
5857
4800
5186
5075
5021
7833
7786
9000
8206
6603
6256
9000
7287
1107
873
1193
1058
8H77833-2timesTo
go-II
4862
3453
5709
4675
5036
4829
4911
4925
6190
5500
9000
6897
5214
3339
9000
5851
979
670
1107
919
9H77833-2times99HS-18
4575
4418
5447
4813
5041
5074
5006
5040
5800
5073
9000
6625
4846
4133
9000
5993
978
733
1135
949
10H7729-2timesG73-107
5615
4962
6273
5617
5023
5535
5165
5241
6143
5501
9000
6881
5540
4441
9000
6327
1000
720
1363
1028
11H7729-2times77245
5472
5164
6281
5639
4745
5031
5028
4935
5572
5175
9000
6582
4901
4345
9000
6082
880
620
897
799
12H7729-2timesCV
J-2-5-3-1-3
5494
5124
6536
5718
4804
5316
4957
5026
7596
8076
9000
8224
6093
5680
9000
6924
981
932
1451
1121
13H7729-2times1305
3804
3826
5511
4380
3596
4724
4387
4236
6362
7075
9000
7479
4230
4517
9000
5916
926
725
1497
1049
14H7729-2times(77371times
BSEC
TCP
-1)
6369
4847
7412
6209
4847
5351
5246
5148
6377
6031
9000
7136
5683
4230
9000
6304
977
671
1380
1009
15H7729-2times96AC
-93
5314
4657
6702
5558
4514
5295
5102
4971
5612
4856
9000
6489
4613
3603
9000
5739
846
679
1397
974
16H7729-2timesTo
go-II
5827
5594
6651
6024
5072
5279
5271
5207
6563
5371
9000
6978
5763
4652
9000
6472
873
719
1040
877
17H7729-2times99HS-18
5741
4638
7918
6099
4847
5024
5021
4964
6179
5603
9000
6927
4882
3725
9000
5869
1012
730
1600
1114
18G73-107times77245
5115
4646
6103
5288
4977
5242
5450
5223
7006
6063
9000
7356
5685
4594
9000
6426
1021
790
1292
1034
19G73-107timesCV
J-2-5-3-1-3
5134
4894
6427
5485
4901
5317
5181
5133
6637
6124
9000
7254
5253
4710
9000
6321
1039
863
1254
1052
20G73-107times1305
4881
4561
7597
5680
4717
4791
4775
4761
6704
6380
9000
7361
4556
4115
9000
5890
1140
882
1657
1226
21G73-107times(77371times
BSEC
TCP
-1)
5636
5519
6283
5812
4986
5219
5203
5136
7990
6203
9000
7731
6620
5436
9000
7019
977
703
1395
1025
22G73-107times96AC
-93
5740
4510
6415
5555
5129
4982
5195
5102
6978
5556
9000
7178
6156
4019
9000
6392
1077
860
1264
1067
23G73-107timesTo
go-II
4460
4357
6044
4953
4396
5138
4951
4829
5279
4835
9000
6371
4018
3603
9000
5540
906
881
1189
992
24G73-107times99HS-18
5473
4356
6374
5401
5102
4955
5055
5037
6460
5197
9000
6886
5534
3704
9000
6079
1171
766
1635
1191
2577245timesCV
J-2-5-3-1-3
4766
4666
5921
5117
5170
4918
4900
4996
5928
5859
9000
6929
4785
4613
9000
6133
984
920
1194
1033
2677245times1305
4575
4060
6052
4895
5128
5297
5203
5210
5102
4990
9000
6364
3979
3464
9000
5481
1279
823
1550
1217
2777245times(77371times
BSEC
TCP
-1)
5017
4929
6022
5322
5108
5473
5274
5285
5308
5044
9000
6451
4498
4191
9000
5896
833
588
939
787
Advances in Agriculture 7
(a)Con
tinued
Sr
No
Genotypes
Germination(
)Ra
teof
emergencelowast
STIlowast
()
SSTIlowast(
)Num
bero
fleavesseedlin
gE 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
2877245times96AC
-93
5472
4705
622154
66
5135
5197
5006
5113
5247
4843
9000
6363
4708
3784
9000
5831
795
662
1058
838
2977245timesTo
go-II
5252
4492
5889
5211
5188
4861
5074
5041
4885
4263
9000
6049
4421
3379
9000
5600
977
751
1243
990
3077245times99HS-18
5740
4099
6872
5570
5249
4898
5258
5135
5628
4616
9000
6415
4863
3014
9000
5626
1045
863
1184
1031
31CV
J-2-5-3-1-3times1305
4364
3944
5700
4669
4974
4517
4650
4714
6097
5579
9000
6892
4594
3842
9000
5812
936
848
1403
1063
32CV
J-2-5-3-1-3times(77371times
BSEC
TCP
-1)4345
3808
6774
4976
5272
4996
5035
5101
6650
5766
9000
7139
4288
3381
9000
5556
800
712
1013
841
33CV
J-2-5-3-1-3times96AC
-93
4862
4197
5743
4934
4884
4980
5058
4974
7477
6760
9000
7745
5957
4709
9000
6555
933
738
1478
1050
34CV
J-2-5-3-1-3timesTo
go-II
4670
4343
6233
5082
4399
4813
4631
4614
5806
6614
9000
7140
4422
4479
9000
5967
963
855
1180
999
35CV
J-2-5-3-1-3times99HS-18
5493
5080
7273
5949
5276
4984
5162
5141
7322
6590
9000
7637
5534
4766
9000
6433
998
807
1338
1047
361305times(77371times
BSEC
TCP
-1)
4613
4539
5717
4956
4986
4955
5080
5007
5137
4870
9000
6336
4210
3936
9000
5715
863
630
913
802
371305times96AC
-93
5473
5177
6830
5827
5224
4779
4919
4974
7117
6965
9000
7694
5640
5220
9000
6620
875
694
1476
1015
381305timesTo
go-II
4230
3420
5262
4304
4309
456144
45
4438
5781
4373
9000
6385
4575
2887
9000
5487
637
566
794
666
391305times99HS-18
5433
4507
6947
5629
5225
4753
4991
4989
7287
6968
9000
7752
5576
4479
9000
6352
1115
818
1410
1114
40(77371times
BSEC
TCP
-1)times
96AC
-93
5595
4429
7461
5828
5216
4955
5121
5097
7087
6368
9000
7485
5432
4038
9000
6157
955
753
1462
1057
41(77371times
BSEC
TCP
-1)times
Togo-II
5252
4728
6106
5362
5492
4808
5132
5144
8025
7505
9000
8177
6310
5413
9000
6908
859
693
990
848
42(77371times
BSEC
TCP
-1)times
99HS-18
5595
4298
6295
5396
4729
4887
5031
4883
7366
6945
9000
7770
6312
4556
9000
6623
903
622
1125
883
4396AC
-93timesTo
go-II
5017
3977
6486
5160
5019
5195
5237
5150
5922
5486
9000
6803
4689
3484
9000
5724
1007
728
1167
967
4496AC
-93times99HS-18
5595
4812
6924
5777
5036
5385
5205
5209
7411
5378
9000
7263
5873
3979
9000
6284
1013
652
1334
1000
45To
go-IItimes
99HS-18
4670
4497
7293
5487
4718
4319
4796
4611
5688
5431
9000
6706
3980
3665
9000
5548
939
653
1462
1018
Mean
5043
4482
6271
5265
4792
498149
23
4899
6377
584190
00
7072
5079
4190
9000
6090
898
701
1168
922
SE097
106
161
mdash213
191
190
mdash19
818
7mdash
mdash14
914
6mdash
mdash036
047
068
mdashCD
272
296
453
mdash597
537
534
mdash555
525
mdashmdash
421
409
mdashmdash
103
133
192
mdashlowastTransfo
rmed
data
(b)
Sr
No
Genotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
908
785
2245
1313
859
1537
1924
1440
154
143
160
152
6267
4800
3833
4967
212
227
249
229
15644
15458
13267
1479
02
H7729-2
1125
1175
1789
1363
317
2400
2811
1843
093
191
252
179
6700
5333
5100
5711
185
210
234
210
18850
18565
15389
17601
3G73-107
1166
1475
1966
1536
943
1744
4945
2544
143
129
366
213
6700
5433
5300
5811
218
219
238
225
15009
1496
513503
14492
477245
1438
1373
2085
1632
607
1863
5957
2809
117
180
509
269
6100
4800
5100
5333
232
221
253
235
14036
13802
11742
1319
35
CVJ-2-5-3-1-3
1214
1437
1807
1486
1163
1610
4166
2313
171
141
407
240
6300
4933
4600
5278
239
227
270
245
1994
317261
1497
017391
61305
858
1137
1737
1244
540
967
4861
2123
125
088
417
210
6533
5033
4800
5456
244
213
257
238
15527
15405
12274
14402
8 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
777371timesBS
ECT
CP-1
1377
1487
1892
1585
928
2660
3330
2306
150
258
301
236
5733
4533
4700
4989
242
220
293
252
1513
814550
1315
014279
896AC
-93
1303
1259
1852
1471
1056
1445
5972
2825
185
147
586
306
5500
4833
3833
4722
256
304
318
293
15577
14032
10000
13203
9To
go-II
1056
1220
2272
1516
924
1466
9113
3834
146
129
883
386
6167
4533
4600
5100
185
149
124
153
14578
12073
10033
12228
1099HS-18
1277
1075
1808
1387
1241
2833
7070
3715
228
226
615
356
6100
5000
4167
5089
243
173
324
247
12627
10355
1017
311052
1H77833-2times
H7729-2
1432
1498
2233
1721
1350
4405
8373
4709
159
499
782
480
5767
4433
3800
4667309
290
248
282
21010
18071
15847
18309
2H77833-2times
G73-107
1675
1635
2205
1838
1236
3211
5789
3412
134
313
547
331
5833
4767
3833
4811
262
295
236
265
21253
20449
15808
1917
0
3H77833-2times
77245
1577
1522
2263
1787
1058
6933
8719
5570
255
553
782
530
5267
4633
4600
4833
273
201
265
246
2074
119511
15208
18487
4H77833-2times
CVJ-2-5-3-1-3
1791
1757
2165
1904
20218030
5276
5109
354
779
477
537
5533
4233
3767
4511
165
330
351
282
21827
20072
17110
19670
5H77833-2times1305
1700
1842
28412128
1427
6228
9134
5596
570
579
813
654
5800
4267
3333
4467340
426
451
406
19816
19444
16037
18432
6H77833-2times
(77371times
BSEC
TCP
-1)
1453
1515
3120
2029
789
465510428
5291
211
408
858
492
5233
4167
3267
4222
203
283
267
251
20677
1913
816470
1876
2
7H77833-2times
96AC
-93
1835
1783
2427
2015
2532
6649
1610
684
29
350
650
1473
824
5467
4233
3700
4467243
239
256
246
22278
20434
15735
19482
8H77833-2times
Togo-II
1564
15632500
1876
1882
4772
11660
6105
296
469
972
579
5667
4533
440048
67
169
260
211
213
2412
82375
619389
22424
9H77833-2times
99HS-18
159318002049
1814
1571
480214024
6799
203
544
1547
765
6033
460040
0048
78
261
225
256
247
23466
21081
1793
320
827
10H7729-2times
G73-107
14951568
2075
1713
959
5157
8517
4877
218
382
666
422
6133
5067
4833
5344
300
273
239
271
23651
2191
717083
20884
11H7729-2times77245
1557
1537
2613
1902
1851
5756
5729
4445366
562
524
484
5633
4800
4633
5022
133
233
297
221
1979
219914
15250
18319
12H7729-2times
CVJ-2-5-3-1-3
18442033
28932257
1651
806114885
8199
264
597
1054
638
6333
4600
4233
5056
170
211
217
199
24629
23339
1970
022556
13H7729-2times1305
1325
1677309920
34
1756
724314048
7682
318
635
1232
728
630046
67
3633
4867298
261
361
307
21666
19371
17422
19486
14H7729-2times(77371
timesBS
ECTCP
-1)
1958
1887
3105
2317
1118
4675
14411
6734
242
457
1318
672
5900
4500
3800
4733
164
209
240
204
23661
1995
419250
20955
15H7729-2times
96AC
-93
1512
1717
2779
2002266844
08
11393
6156
356
500
1211
689
5633
4533
4200
4789
221
195
282
232
2194
921635
17213
20266
Advances in Agriculture 9
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
16H7729-2times
Togo-II
1519
1738
2159
1805
2437
9867
1616
294
89
361
961
1527
950
6167
4700
4200
5022
234
272
230
246
26338
2414
520553
23678
17H7729-2times
99HS-18
1938
1787
3151
2292
1574
6218
11318
6370
290
606
956
617
6067
4767
406749
67
172
210
199
194
24689
21854
1778
921444
18G73-107times77245
1856
1813
2210
1960
2435
8550
7828
6271338
633
612
528
5500
4633
440048
44
199
255
307
254
1993
619277
16095
18436
19G73-107times
CVJ-2-5-3-1-3
1517
1769
2407
1897
1556
7728
9136
6140
239
876
917
677
5900
4300
3400
4533
233
266
315
272
23418
22333
19317
21689
20G73-107times1305
1217
1507
2832
1852
1777
5189
1114
960
38
289
588
1197
691
6733
5000
4233
5322
286
210
312
269
2078
81992
817067
19261
21G73-107times(77371
timesBS
ECTCP
-1)
18551946
2730
2177
1801
3482
10415
5233
239
277
753
423
5500
4467
3433
4467243
197
273
238
23817
20263
17425
20502
22G73-107times
96AC
-93
1718
1840
28612140
1345
9333
14669
8449
297
845
1278
807
5600
5033
4633
5089
270
243
300
271
28537
21461
16875
22291
23G73-107timesTo
go-II
168018083087
2192
1112
4678
9319
5036
186
473
985
548
6033
4767
4200
5000
210
209
231
217
24864
24609
21625
23699
24G73-107times99HS-18
1398
1675
3370
2148
2753
8672
11918
7781
362
691
981
678
566746
00
3500
4589
170
247
270
229
24677
20567
17855
21033
2577245times
CVJ-2-5-3-1-3
1500
1728
2203
1810
2282
4679
11492
6151
328
424
1116
623
6000
4333
420048
44300
231
370
300
21257
20497
1772
519826
2677245times1305
143815772080
1698
949
10439
12053
7814
121
915
992
676
5767
4800
3500
4689
168246
316
244
1978
91779
015517
17699
2777245times(77371times
BSEC
TCP
-1)
150516972846
2016
3222
8518
12621
8120
469
827
1141
812
5400
4367
3833
4533
196
225
242
221
2092
819485
1719
219202
2877245times96AC
-93
1653
1797
2117
1856
1934
6056
11543
6511
352
563
911
608
5333
4267
3833
4478
186
169
198
184
22003
19054
1676
719275
2977245timesTo
go-II
1723
1718
3163
2201
1749
8250
15443
8481229
806
1412
816
5967
4700
4067
4911
174
245
298
239
22075
23503
19875
21818
3077245times99HS-18
2016
1970
28542280
3217
13027
3531
6591395
965
285
549
5733
4467
3833
4678
148
228
209
195
2212
721462
1690
020
163
31CV
J-2-5-3-1-3times
1305
1358
1422
2179
1653
1254
2803
1019
44750
178
254
801
411
6033
4467
3367
4622
312
193
330
278
22269
19547
17250
19689
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
1335
1742
2400
1826
922
5226
12806
6318
236
458
1054
583
5633
4433
3600
4556
321
315
259
298
2210
419524
17087
19572
33CV
J-2-5-3-1-3times
96AC
-93
1552
16803260
2164
166710094
11941
7901250
974
1115
779
5533
4533
446748
44
170
202
317
230
2392
123317
19333
2219
0
34CV
J-2-5-3-1-3times
Togo-II
142216072638
1889
1012
4163
11061
5412
261
377
891
510
6167
4967
4533
5222
207
225
325
252
23590
22860
20278
22243
35CV
J-2-5-3-1-3times
99HS-18
1435
1600
2700
1912
3056
3237
7089
4461449
301
658
469
5700
44673800
4656
148
178
225
183
22444
20240
1696
719884
361305times(77371times
BSEC
TCP
-1)
1499
1767
2058
1774
1882
4665
7920
4822
356
426
724
502
566742673800
4578
271
242
305
273
22011
19295
16417
19241
371305times96AC
-93
16781605
2757
2014
1516
10037
11960
7838
311
11381284
911
560040
673400
4356
182
276
251
236
2396
519625
15800
1979
738
1305timesTo
go-II
1177
16802249
1702
902
4676
9388
4989
241
456
795
497
6533
5533
4733
5600
214
266
296
259
21894
20217
19222
20444
391305times99HS-18
1774
1767
2766
2102
2145
4628
10618
5797
301
447
991
580
5933
4500
3733
4722
128
142
180
150
2275
719413
16967
1971
2
40(77371times
BSEC
TCP
-1)times
96AC
-93
1748
1795
31712238
1537
12586
14379
9501
211
1104
1183
833
546744
00346744
44
127
201
226
185
23294
21089
17858
2074
7
10 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(
days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
41(77371times
BSEC
TCP
-1)times
Togo-II
19652007
2653
2208
2136
5601
9503
5747
315
635
1001
650
5533
4833
410048
22
213
241
286
247
22662
22094
17667
20808
42(77371times
BSEC
TCP
-1)times
99HS-18
15011687
2705
1964
1935
7253
11806
6998
314
704
1067
695
5467
4433
5133
5011
177
167
175
173
22305
19277
16392
19325
4396AC
-93timesTo
go-II1759
1618
2123
1834
1539
10347
12212
8033
325
907
1090
774
5900
450039674789
214
269
339
274
23252
21554
16875
20560
4496AC
-93times
99HS-18
1353
1618
2925
1966
3532
7223
11950
7569
440
654
1024
706
5600
4900
4533
5011
149
175
206
177
24494
20698
18111
2110
145
Togo-IItimes
99HS-18
1117
1278
2582
1659
3448
6764
11910
7374
477
767
1207
817
6000
5133
4333
5156
183
203
216
201
17683
15541
14333
15853
Mean
1513
1618
2492
1874
1656
5773
9854
5761
272
538
885
565
586846
534116
4879
218
233
268
240
21297
19547
16529
1912
4SE
101
073
098
mdash079
152
317
mdash014
014
028
mdash351
365
428
mdash036
039
046
mdash985
1173
1211
mdashCD
284
205
276
mdash221
427
890
mdash038
040
079
mdash12
513
015
2mdash
013
014
016
mdash351
418
431
mdash
(c)
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
1642
1667
1744
1684
1504
1349
1526
1460
2783
2405
2634
2607
836
745
908
830
4287
3754
4160
4067
2H7729-2
1693
1817
1950
1820
1012
859
1016
962
3433
2809
2591
2944
649
597
651
632
4445
3668
3607
3906
3G73-107
2000
2028
2077
2035
2280
1966
2312
2186
4695
4023
4229
4316
1281
1170
1463
1305
6975
5989
6541
6502
477245
1523
1630
1728
1627
1796
2489
2702
2329
5423
4851
4498
4924
748
1464
1743
1319
7219
7341
7200
7253
5CV
J-2-5-3-1-3
2282
2692
2787
2587
2411
2643
2901
2652
5857
4836
5024
5239
1206
1335
1769
1437
8268
7479
7925
7891
61305
1851
1951
2054
1952
2026
1763
1908
1899
4657
4414
4463
4511
1468
1336
1539
1448
6683
6177
6371
6410
777371timesBS
ECTCP
-11629
1645
1733
1669
1244
1692
1884
1607
4925
4183
4435
4515
625
1092
1483
1067
6169
5875
6319
6121
896AC
-93
1379
1519
1600
1499
2066
2942
3088
2698
4501
4095
4229
4275
984
1565
1678
1409
6567
7037
7316
6973
9To
go-II
1904
2057
2103
2021
2167
1844
2358
2123
4520
3446
3904
3957
1298
1138
1502
1313
6687
5290
6262
6080
1099HS-18
1475
1485
1633
1531
3180
2904
3382
3155
5633
4139
5205
4992
1656
1536
1879
1690
8813
7043
8587
8147
1H77833-2timesH7729-2
2010
2046
2133
2063
3645
3075
4377
3699
7675
6540
7199
7138
2014
1708
2880
2201
11320
9615
11576
10837
2H77833-2timesG73-107
2066
2101
2122
2096
5002
4053
4601
4552
7882
7430
6963
7425
3146
2565
2808
2840
12884
11482
11564
11977
3H77833-2times77245
1764
2048
2083
1965
3769
4497
4559
4275
8681
8515
8475
8557
2284
3356
3377
3006
12450
13012
13034
12832
4H77833-2times
CVJ-2-5-3-1-3
2205
2383
2733
2441
3693
5655
5220
4856
11656
10287
1010
510683
2198
4488
4176
3621
15348
1594
215325
15538
5H77833-2times1305
2209
2423
2491
2374
6008
5842
6933
6261
15238
14311
13889
14479
3758
4057
5591
4469
21246
2015
320822
2074
0
6H77833-2times(77371times
BSEC
TCP
-1)
1789
1823
1992
1868
3593
4288
4903
4262
8922
6522
7429
7625
2178
3224
3070
2824
12515
10811
12333
11886
7H77833-2times96AC
-93
1739
1960
2011
1903
4557
4350
4818
4575
7916
6068
7290
7092
2411
2529
3191
2710
12474
10419
1210
811667
8H77833-2timesTo
go-II
1927
2389
2406
2241
4185
5828
6215
5409
12319
10279
11023
11207
2433
4254
4631
3773
16504
1610
717238
16616
9H77833-2times99HS-18
1941
2245
2330
2172
4227
4139
4529
4298
9211
8120
7969
8433
3154
3184
3253
3197
13438
12259
12498
1273
210
H7729-2timesG73-107
1968
2109
2158
2079
3725
3042
3488
3418
7313
5956
6174
6481
2822
2001
2357
2393
11038
8998
9662
9899
Advances in Agriculture 11
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
11H7729-2times77245
1847
2048
2200
2032
2467
3759
5466
3898
7672
7449
8921
8014
1356
2350
3796
2500
1013
911208
14387
11911
12H7729-2times
CVJ-2-5-3-1-3
2218
2585
2767
2523
3674
4372
4483
4176
10053
9467
8664
9395
2417
3312
3345
3025
1372
813839
1314
713571
13H7729-2times1305
2169
2338
2547
2352
6728
6166
6792
6562
13333
11662
13301
1276
54128
4501
5660
4763
20061
17828
20093
19327
14H7729-2times(77371times
BSEC
TCP
-1)
1925
2095
2127
2049
3530
4165
4649
4115
9638
6917
7698
8084
1681
2833
3150
2555
1316
8110
82
12347
1219
915
H7729-2times96AC
-93
2012
2070
2180
2087
4955
4447
4596
4666
8738
6882
7762
7794
3754
3558
3181
3498
13693
11329
12359
12460
16H7729-2timesTo
go-II
2138
2428
2563
2377
4949
4660
5118
4909
8549
7243
7191
7661
3586
3353
3028
3322
13498
11903
12310
12570
17H7729-2times99HS-18
1899
1933
2073
1969
4046
4527
4988
4520
8492
7895
8144
8177
2561
3378
3553
3164
12539
12422
1313
212698
18G73-107times77245
1840
2253
2437
2177
2580
4776
5915
4424
11673
10608
10459
10913
1358
3163
4286
2936
14253
15385
16374
15337
19G73-107times
CVJ-2-5-3-1-3
2324
2537
2691
2517
4958
5030
5599
5196
9524
9395
1114
610022
3241
3645
4444
3776
14483
14425
1674
515217
20G73-107times1305
2228
2433
2583
2415
6340
5794
6373
6169
12490
1015
8119
61
11536
4199
4199
5018
4472
18830
1595
218334
1770
6
21G73-107times(77371times
BSEC
TCP
-1)
1943
2128
2220
2097
3936
3980
5770
4562
11697
9858
10303
10619
1874
2369
4181
2808
15633
13839
16073
1518
222
G73-107times96AC
-93
2118
2218
2317
2217
4270
5843
6201
5438
10319
9872
1015
910117
2703
4460
4379
3847
14589
15715
16359
15555
23G73-107timesTo
go-II
2237
2365
2733
2445
5736
5175
6822
5911
12098
10808
1299
611967
3984
3670
5372
4342
17835
1598
219818
17878
24G73-107times99HS-18
2025
2081
2322
2143
3558
4229
6612
4800
12350
9685
11516
1118
42196
2782
5009
3329
1590
813914
1812
915983
2577245timesCV
J-2-5-3-1-3
2109
2360
2675
2381
6760
6558
7299
6872
15225
11861
1416
21374
94225
4752
5839
4939
2198
518418
21461
20621
2677245times1305
1919
2100
2197
2072
5700
5142
5347
5397
11687
10565
10737
10996
4318
3956
4244
4173
17386
1570
816085
16393
2777245times(77371times
BSEC
TCP
-1)
1851
1935
2020
1936
2577
2460
2838
2625
6989
4778
4636
5468
1631
1662
1999
1764
9566
7238
7474
8093
2877245times96AC
-93
1729
1780
2133
1881
5541
5058
5748
5449
11952
8174
9526
9884
3646
3587
4071
3768
17493
13232
15274
15333
2977245timesTo
go-II
2300
2508
2658
2489
3067
5431
5963
4820
11737
11021
1216
411641
1598
4053
4770
3474
14805
16452
1812
616461
3077245times99HS-18
1982
2019
2223
2075
3846
3119
4225
3730
7493
6720
6476
6896
3205
2578
2643
2809
11339
9839
1070
110626
12 Advances in Agriculture
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
31CV
J-2-5-3-1-3times1305
2406
2553
3067
2675
4178
4796
6281
5085
13822
11425
12252
12500
2472
3241
4907
3540
18000
16222
18532
17585
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
2142
2173
2238
2184
4522
5235
5640
5132
11673
9658
10248
10526
2614
3766
4178
3519
1619
514893
15888
15659
33CV
J-2-5-3-1-3times
96AC
-93
2350
2477
2600
2476
4676
4280
6090
5015
11975
9832
10868
10891
3097
2931
4445
3491
16651
14111
1695
815907
34CV
J-2-5-3-1-3times
Togo-II
2093
2666
2989
2583
4058
3727
5190
4325
12588
10717
11217
11507
2742
2570
4185
3166
16645
14443
16407
15832
35CV
J-2-5-3-1-3times
99HS-18
2028
2311
2386
2242
3287
2513
5356
3719
10055
9733
1036
410051
2004
1581
4251
2612
13342
12246
1572
01376
9
361305times(77371times
BSEC
TCP
-1)
2005
2173
2210
2129
3569
4692
5140
4467
11652
9826
10041
10507
2005
3665
4179
3283
15221
14518
1518
214974
371305times96AC
-93
1822
2003
2047
1957
5115
4023
4813
4650
9965
7675
7571
8404
3456
2647
3155
3086
15080
11698
12384
13054
381305timesTo
go-II
2545
2583
2644
2591
6885
7283
6795
6987
12265
11410
12583
12086
4500
6120
5350
5323
1915
018693
19378
19074
391305times99HS-18
2070
2408
2593
2357
5219
4332
4800
4784
1092
97677
8968
9192
3924
3332
3840
3699
1614
912009
1376
913976
40(77371times
BSEC
TCP
-1)
times96AC
-93
2056
2115
2157
2109
3158
4463
4717
4113
9166
7540
8100
8268
1880
3542
3347
2923
12324
12003
12817
12381
41(77371times
BSEC
TCP
-1)
timesTo
go-II
1868
2253
2450
2190
4422
6458
7038
5973
1415
312369
1379
713439
2291
4646
5586
4174
18575
18827
20835
19412
42(77371times
BSEC
TCP
-1)
times99HS-18
1995
2026
2209
2077
2720
2909
3543
3057
7392
4692
5023
5702
1766
2187
2147
2033
10112
7601
8566
8760
4396AC
-93timesTo
go-II
1953
2027
2511
2164
5972
4391
7126
5830
15537
1392
714432
14632
3780
2538
5655
3991
21510
18318
21558
20462
4496AC
-93times99HS-18
1952
2021
2242
2072
3465
2758
4496
3573
5959
5186
6094
5746
2707
2154
2409
2423
9424
7944
10590
9319
45To
go-IItimes
99HS-18
1932
2008
2658
2199
5381
4995
5436
5270
10299
8876
9244
9473
3986
3842
3668
3832
15680
13871
14680
1474
3Mean
1982
2146
2301
2143
3963
4123
4836
4307
9498
8087
8663
8749
2510
2914
3513
2979
13460
12210
13499
13056
SE243
198
245
mdash671
501
626
mdash959
750
822
mdash423
349
344
mdash1155
927
1167
mdashCD
087
071
087
mdash239
178
223
mdash342
267
293
mdash15
112
412
2mdash
411
330
416
mdash
Advances in Agriculture 13Ta
ble4Analysis
ofvaria
ncefor
10times10
half-diallelfor
different
charactersin
threee
nviro
nments
(a)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Germination
()
Rateof
emergence
STI()
SSTI
()
Num
bero
fleavesseedling
Seedlin
gheight
(cm)
Freshweight
(g)seedlin
gDry
weight
(g)seedlin
g
E 1
Replicates
2652731
596768
783393
20700
93018674
33764
1033999
002590
Treatm
ents
541097
0255lowastlowast
8181153lowastlowast
30863092lowastlowast
20680900lowastlowast
1100708lowastlowast
2106317lowastlowast
930829527lowastlowast
319767lowastlowast
Parents
95118
163lowastlowast
14289925lowastlowast
59114816lowastlowast
18880254lowastlowast
1084227lowastlowast
1087906lowastlowast
22561719lowastlowast
042162lowastlowast
Hybrid
s44
11423573lowastlowast
3398933lowastlowast
25697199lowastlowast
20633716lowastlowast
472874lowastlowast
1391526lowastlowast
674123518lowastlowast
261839lowastlowast
Parentsv
ersush
ybrid
s1
43693104lowastlowast
163619
916lowastlowast
3896838
3896
2793lowastlowast
28873741lowastlowast
4272
2798lowastlowast
234144289lowastlowast
5367022lowastlowast
Error
108
281184
1356832
1173684
674337
040104
306
462
2015
1619
005535
E 2
Replicates
2583819
3707389lowast
1596635
198738
034925
448213
899529
007177
Treatm
ents
549997443lowastlowast
2825177lowastlowast
35378177lowastlowast
19074306lowastlowast
665284lowastlowast
1764
081lowastlowast
257694570lowastlowast
2177519lowastlowast
Parents
96507784lowastlowast
3718
879lowastlowast
69866289lowastlowast
1798
5404lowastlowast
705379lowastlowast
1408518lowastlowast
10612519lowastlowast
077429lowastlowast
Hybrid
s44
10569731lowastlowast
2127302lowastlowast
2910
2537lowastlowast
19429240lowastlowast
329256lowastlowast
7000
03lowastlowast
186026552lowastlowast
1483428lowastlowast
Parentsv
ersush
ybrid
s1
16223710lowastlowast
25488361lowastlowast
1113
326
13257362lowastlowast
15089673lowastlowast
5178
3569lowastlowast
5634825791lowastlowast
51618348lowastlowast
Error
108
334760
1097039
1049505
635570
06764
116
0576
694613
006206
E 3
Replicates
2894398
4808815lowast
mdashmdash
051076
095989
3768421
030220
Treatm
ents
541261560
0lowastlowast
4842216lowastlowast
mdashmdash
2811077lowastlowast
5947316lowastlowast
3870844
50lowastlowast
3445076lowastlowast
Parents
97135
735lowastlowast
1097
6388lowastlowast
mdashmdash
1468699lowastlowast
1112
764lowastlowast
136081650lowastlowast
1307836lowastlowast
Hybrid
s44
13217740lowastlowast
1379360
mdashmdash
15540
71lowastlowast
4581632lowastlowast
252084767lowastlowast
2378032lowastlowast
Parentsv
ersush
ybrid
s1
35440
180lowastlowast
102000335lowastlowast
mdashmdash
70200767lowastlowast
109548362lowastlowast
8586095705lowastlowast
69630188lowastlowast
Error
108
782907
1085444
mdashmdash
139771
291325
3019
169
02400
4(b)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Panicle
emergence
Effectiv
etillersplant
Planth
eight
Earlength
Earw
eightp
lant
Dry
fodd
eryieldplant
Grain
yieldplant
Totalbiological
yieldplant
E 1
Replicates
231860
61lowastlowast
416348lowastlowast
1672
9472lowast
1706
411077192
9634539
392777
115544
59Treatm
ents
544152
368lowastlowast
085657lowastlowast
326706089lowastlowast
1602572lowastlowast
6177
0406lowastlowast
304113
204lowastlowast
34285030lowastlowast
575617
582lowastlowast
Parents
94511481lowastlowast
018538lowastlowast
140807476lowastlowast
2240093lowastlowast
11653
959lowastlowast
27014155lowastlowast
3858650lowastlowast
61321919lowastlowast
Hybrid
s44
3201347lowastlowast
100797lowastlowast
110395657lowastlowast
1010
497lowastlowast
40296671lowastlowast
171271411lowastlowast
2413
6880lowastlowast
302999650lowastlowast
Parentsv
ersush
ybrid
s1
4276
5286lowastlowast
02360
9lowast11517452
616lowastlowast
2191
6167lowastlowast
1457662749lowastlowast
8643043524lowastlowast
7546
41053lowastlowast
17199467546lowastlowast
Error
108
468159
004907
3692099
224610
17114
303498126
682350
5075268
E 2
Replicates
2656364
002556
61575565lowastlowast
828842lowastlowast
589094
2268506
260877
1507116
Treatm
ents
542999618lowastlowast
069822lowastlowast
268998723lowastlowast
2436886lowastlowast
60592784lowastlowast
247259877lowastlowast
4192
0073lowastlowast
513483489lowastlowast
Parents
926300
00lowastlowast
048079lowastlowast
165555
405lowastlowast
3871874lowastlowast
1412
2393lowastlowast
19543682lowastlowast
3132
426lowastlowast
57230706lowastlowast
Hybrid
s44
2533
165lowastlowast
07344
9lowastlowast
9616
2857lowastlowast
1463359lowastlowast
35495366lowastlowast
154759288lowastlowast
26262465lowastlowast
293492304lowastlowast
Parentsv
ersush
ybrid
s1
26850135lowastlowast
105929lowastlowast
880476
6697lowastlowast
32357161lowastlowast
1583112746lowastlowast
6366731560lowastlowast
107994
3646lowastlowast
14299370699lowastlowast
Error
108
507598
005829
5234122
149406
955133
2142411
462311
3273886
E 3
Replicates
2029697
021161
9459847
143746
985141
46800
6492
6334
3345160
Treatm
ents
548045499lowastlowast
094511lowastlowast
192420108lowastlowast
3279225lowastlowast
72049211lowastlowast
298953
021lowastlowast
55061308lowastlowast
651732994lowastlowast
Parents
98010
741lowastlowast
094166lowastlowast
116037343lowastlowast
3702185lowastlowast
16305190lowastlowast
2323644
0lowastlowast
4553
893lowastlowast
71484982lowastlowast
Hybrid
s44
6254377lowastlowast
095514lowastlowast
7378
1513lowastlowast
2188348lowastlowast
31813193lowastlowast
190263916lowastlowast
31572179lowastlowast
368689934lowastlowast
Parentsv
ersush
ybrid
s1
8716
7710lowastlowast
053496lowast
609996
3199lowastlowast
47471212lowastlowast
2344
130203lowastlowast
756272
2861lowastlowast
154314
9707lowastlowast
18327859
719lowastlowast
Error
108
696981
007883
5578505
228608
1491014
2574
223
449588
5187705
lowast
Sign
ificant
at119875=005
lowastlowastSign
ificant
at119875=001
14 Advances in Agriculture
Table 5 Mean performance of the genotypes for membrane thermostability in two environments
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594
Advances in Agriculture 15
Table 5 Continued
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434
Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash
lowastValues are transformed values
0
6
12
18
24
30
36
42
48
54
minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity
G73-10796AC-93CVJ-2-5-3-1-3
Togo-II99HS-18
H77833-2H7729-2772451305
H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305
1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305
H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305
77245 times 1305
77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305
77371 times BSECT CP-1
H77833-2 times G73-107
Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits
moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling
survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The
16 Advances in Agriculture
Table6Po
oled
correla
tioncoeffi
cientamon
gvario
uscharactersin
threee
nviro
nments
Characters
Germination
Rateof
emergence
STI
SSTI
Leaves
seedlin
gSeedlin
gheight
Fresh
weight
Dry
weight
MTS
Panicle
emergence
Effectiv
etillersplant
Plant
height
Earlength
Ear
weightp
lant
Dry
fodd
eryield
plant
Grain
yield
plant
Total
biological
yield
plant
Germination
1000
0357lowastlowast
0353lowastlowast
0590lowastlowast
0230
0373lowastlowast
0293lowast
0260
0276lowastminus0135minus0478lowastlowast
0326lowast
minus0069minus0104
minus0190
minus0145
minus0167
Rateof
emergence
1000
0233
0351lowastlowast
0605lowastlowast
0585lowastlowast
0551lowastlowast
0524lowastlowast
0139minus0593lowastlowast
0032
0517lowastlowast
0217
0388lowastlowast
0361lowastlowast
0348lowastlowast
0377lowastlowast
STI
1000
0837lowastlowast
0241
0129
0081
004
80890lowastlowast
minus0215
minus0211
0030minus0007minus0043
minus0101
minus0111
minus0084
SSTI
1000
0233
0264
0183
0140
0745lowastlowast
minus0247minus0268lowast
0184minus0017minus006
6minus0129
minus0144
minus0112
Num
bero
fleaves
1000
0683lowastlowast
0633lowastlowast
060
4lowastlowast
0176minus0474lowastlowast
0109
0576lowastlowast
0496lowastlowast
064
1lowastlowast
0607lowastlowast
0630lowastlowast
0630lowastlowast
Seedlin
gheight
1000
0703lowastlowast
0663lowastlowast
0031minus0493lowastlowast
minus0115
0735lowastlowast
0360lowastlowast
0526lowastlowast
0520lowastlowast
0515lowastlowast
0532lowastlowast
Freshweight
1000
0957lowastlowast
minus0016
0425lowastlowast
minus0082
0661lowastlowast
0351lowastlowast
0581lowastlowast
0496lowastlowast
0576lowastlowast
0533lowastlowast
Dry
weight
1000minus0033minus0428lowastlowast
minus0016
0634lowastlowast
0354lowastlowast
0620lowastlowast
0509lowastlowast
0614lowastlowast
0554lowastlowast
MTS
1000minus040
minus0177minus0037
0015
minus0074
minus0138
minus0131
minus0120
Panicle
emergence
1000
minus0150minus0269lowast
0022minus0329lowast
minus0337lowast
minus0280lowast
minus0341lowast
Effectiv
etillersplant
1000
minus0012
0170
0366lowastlowast
040
5lowastlowast
0323lowast
0401lowastlowast
Planth
eight
1000
0650lowastlowast
0603lowastlowast
0596lowastlowast
0619lowastlowast
0610lowastlowast
Earlength
1000
0608lowastlowast
0671lowastlowast
064
8lowastlowast
066
4lowastlowast
Earw
eightp
lant
1000
0910lowastlowast
0984lowastlowast
0957lowastlowast
Dry
fodd
eryield
plant
1000
0907lowastlowast
0991lowastlowast
Grain
yield
plant
1000
0950lowastlowast
Totalbiological
yield
plant
1000
lowastSign
ificant
at119875=005lowastlowastSign
ificant
at119875=001
Advances in Agriculture 17
faster germination and early vigour in seedling could act asphysical indicators for heat tolerance
Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits
Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics
Conflict of Interests
The authors declare that there is no conflict of interestsregarding the publication of this paper
References
[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981
[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993
[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006
[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993
[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013
[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007
[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965
[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011
[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001
[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009
[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962
[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967
[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963
[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001
Submit your manuscripts athttpwwwhindawicom
Nutrition and Metabolism
Journal of
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Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Food ScienceInternational Journal of
Agronomy
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
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Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
Microbiology
The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom
Applied ampEnvironmentalSoil Science
Volume 2014
AgricultureAdvances in
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PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
BiodiversityInternational Journal of
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ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
GenomicsInternational Journal of
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Plant GenomicsInternational Journal of
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Biotechnology Research International
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Forestry ResearchInternational Journal of
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Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
EcologyInternational Journal of
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Veterinary Medicine International
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Cell BiologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Evolutionary BiologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Advances in Agriculture 7
(a)Con
tinued
Sr
No
Genotypes
Germination(
)Ra
teof
emergencelowast
STIlowast
()
SSTIlowast(
)Num
bero
fleavesseedlin
gE 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
2877245times96AC
-93
5472
4705
622154
66
5135
5197
5006
5113
5247
4843
9000
6363
4708
3784
9000
5831
795
662
1058
838
2977245timesTo
go-II
5252
4492
5889
5211
5188
4861
5074
5041
4885
4263
9000
6049
4421
3379
9000
5600
977
751
1243
990
3077245times99HS-18
5740
4099
6872
5570
5249
4898
5258
5135
5628
4616
9000
6415
4863
3014
9000
5626
1045
863
1184
1031
31CV
J-2-5-3-1-3times1305
4364
3944
5700
4669
4974
4517
4650
4714
6097
5579
9000
6892
4594
3842
9000
5812
936
848
1403
1063
32CV
J-2-5-3-1-3times(77371times
BSEC
TCP
-1)4345
3808
6774
4976
5272
4996
5035
5101
6650
5766
9000
7139
4288
3381
9000
5556
800
712
1013
841
33CV
J-2-5-3-1-3times96AC
-93
4862
4197
5743
4934
4884
4980
5058
4974
7477
6760
9000
7745
5957
4709
9000
6555
933
738
1478
1050
34CV
J-2-5-3-1-3timesTo
go-II
4670
4343
6233
5082
4399
4813
4631
4614
5806
6614
9000
7140
4422
4479
9000
5967
963
855
1180
999
35CV
J-2-5-3-1-3times99HS-18
5493
5080
7273
5949
5276
4984
5162
5141
7322
6590
9000
7637
5534
4766
9000
6433
998
807
1338
1047
361305times(77371times
BSEC
TCP
-1)
4613
4539
5717
4956
4986
4955
5080
5007
5137
4870
9000
6336
4210
3936
9000
5715
863
630
913
802
371305times96AC
-93
5473
5177
6830
5827
5224
4779
4919
4974
7117
6965
9000
7694
5640
5220
9000
6620
875
694
1476
1015
381305timesTo
go-II
4230
3420
5262
4304
4309
456144
45
4438
5781
4373
9000
6385
4575
2887
9000
5487
637
566
794
666
391305times99HS-18
5433
4507
6947
5629
5225
4753
4991
4989
7287
6968
9000
7752
5576
4479
9000
6352
1115
818
1410
1114
40(77371times
BSEC
TCP
-1)times
96AC
-93
5595
4429
7461
5828
5216
4955
5121
5097
7087
6368
9000
7485
5432
4038
9000
6157
955
753
1462
1057
41(77371times
BSEC
TCP
-1)times
Togo-II
5252
4728
6106
5362
5492
4808
5132
5144
8025
7505
9000
8177
6310
5413
9000
6908
859
693
990
848
42(77371times
BSEC
TCP
-1)times
99HS-18
5595
4298
6295
5396
4729
4887
5031
4883
7366
6945
9000
7770
6312
4556
9000
6623
903
622
1125
883
4396AC
-93timesTo
go-II
5017
3977
6486
5160
5019
5195
5237
5150
5922
5486
9000
6803
4689
3484
9000
5724
1007
728
1167
967
4496AC
-93times99HS-18
5595
4812
6924
5777
5036
5385
5205
5209
7411
5378
9000
7263
5873
3979
9000
6284
1013
652
1334
1000
45To
go-IItimes
99HS-18
4670
4497
7293
5487
4718
4319
4796
4611
5688
5431
9000
6706
3980
3665
9000
5548
939
653
1462
1018
Mean
5043
4482
6271
5265
4792
498149
23
4899
6377
584190
00
7072
5079
4190
9000
6090
898
701
1168
922
SE097
106
161
mdash213
191
190
mdash19
818
7mdash
mdash14
914
6mdash
mdash036
047
068
mdashCD
272
296
453
mdash597
537
534
mdash555
525
mdashmdash
421
409
mdashmdash
103
133
192
mdashlowastTransfo
rmed
data
(b)
Sr
No
Genotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
908
785
2245
1313
859
1537
1924
1440
154
143
160
152
6267
4800
3833
4967
212
227
249
229
15644
15458
13267
1479
02
H7729-2
1125
1175
1789
1363
317
2400
2811
1843
093
191
252
179
6700
5333
5100
5711
185
210
234
210
18850
18565
15389
17601
3G73-107
1166
1475
1966
1536
943
1744
4945
2544
143
129
366
213
6700
5433
5300
5811
218
219
238
225
15009
1496
513503
14492
477245
1438
1373
2085
1632
607
1863
5957
2809
117
180
509
269
6100
4800
5100
5333
232
221
253
235
14036
13802
11742
1319
35
CVJ-2-5-3-1-3
1214
1437
1807
1486
1163
1610
4166
2313
171
141
407
240
6300
4933
4600
5278
239
227
270
245
1994
317261
1497
017391
61305
858
1137
1737
1244
540
967
4861
2123
125
088
417
210
6533
5033
4800
5456
244
213
257
238
15527
15405
12274
14402
8 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
777371timesBS
ECT
CP-1
1377
1487
1892
1585
928
2660
3330
2306
150
258
301
236
5733
4533
4700
4989
242
220
293
252
1513
814550
1315
014279
896AC
-93
1303
1259
1852
1471
1056
1445
5972
2825
185
147
586
306
5500
4833
3833
4722
256
304
318
293
15577
14032
10000
13203
9To
go-II
1056
1220
2272
1516
924
1466
9113
3834
146
129
883
386
6167
4533
4600
5100
185
149
124
153
14578
12073
10033
12228
1099HS-18
1277
1075
1808
1387
1241
2833
7070
3715
228
226
615
356
6100
5000
4167
5089
243
173
324
247
12627
10355
1017
311052
1H77833-2times
H7729-2
1432
1498
2233
1721
1350
4405
8373
4709
159
499
782
480
5767
4433
3800
4667309
290
248
282
21010
18071
15847
18309
2H77833-2times
G73-107
1675
1635
2205
1838
1236
3211
5789
3412
134
313
547
331
5833
4767
3833
4811
262
295
236
265
21253
20449
15808
1917
0
3H77833-2times
77245
1577
1522
2263
1787
1058
6933
8719
5570
255
553
782
530
5267
4633
4600
4833
273
201
265
246
2074
119511
15208
18487
4H77833-2times
CVJ-2-5-3-1-3
1791
1757
2165
1904
20218030
5276
5109
354
779
477
537
5533
4233
3767
4511
165
330
351
282
21827
20072
17110
19670
5H77833-2times1305
1700
1842
28412128
1427
6228
9134
5596
570
579
813
654
5800
4267
3333
4467340
426
451
406
19816
19444
16037
18432
6H77833-2times
(77371times
BSEC
TCP
-1)
1453
1515
3120
2029
789
465510428
5291
211
408
858
492
5233
4167
3267
4222
203
283
267
251
20677
1913
816470
1876
2
7H77833-2times
96AC
-93
1835
1783
2427
2015
2532
6649
1610
684
29
350
650
1473
824
5467
4233
3700
4467243
239
256
246
22278
20434
15735
19482
8H77833-2times
Togo-II
1564
15632500
1876
1882
4772
11660
6105
296
469
972
579
5667
4533
440048
67
169
260
211
213
2412
82375
619389
22424
9H77833-2times
99HS-18
159318002049
1814
1571
480214024
6799
203
544
1547
765
6033
460040
0048
78
261
225
256
247
23466
21081
1793
320
827
10H7729-2times
G73-107
14951568
2075
1713
959
5157
8517
4877
218
382
666
422
6133
5067
4833
5344
300
273
239
271
23651
2191
717083
20884
11H7729-2times77245
1557
1537
2613
1902
1851
5756
5729
4445366
562
524
484
5633
4800
4633
5022
133
233
297
221
1979
219914
15250
18319
12H7729-2times
CVJ-2-5-3-1-3
18442033
28932257
1651
806114885
8199
264
597
1054
638
6333
4600
4233
5056
170
211
217
199
24629
23339
1970
022556
13H7729-2times1305
1325
1677309920
34
1756
724314048
7682
318
635
1232
728
630046
67
3633
4867298
261
361
307
21666
19371
17422
19486
14H7729-2times(77371
timesBS
ECTCP
-1)
1958
1887
3105
2317
1118
4675
14411
6734
242
457
1318
672
5900
4500
3800
4733
164
209
240
204
23661
1995
419250
20955
15H7729-2times
96AC
-93
1512
1717
2779
2002266844
08
11393
6156
356
500
1211
689
5633
4533
4200
4789
221
195
282
232
2194
921635
17213
20266
Advances in Agriculture 9
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
16H7729-2times
Togo-II
1519
1738
2159
1805
2437
9867
1616
294
89
361
961
1527
950
6167
4700
4200
5022
234
272
230
246
26338
2414
520553
23678
17H7729-2times
99HS-18
1938
1787
3151
2292
1574
6218
11318
6370
290
606
956
617
6067
4767
406749
67
172
210
199
194
24689
21854
1778
921444
18G73-107times77245
1856
1813
2210
1960
2435
8550
7828
6271338
633
612
528
5500
4633
440048
44
199
255
307
254
1993
619277
16095
18436
19G73-107times
CVJ-2-5-3-1-3
1517
1769
2407
1897
1556
7728
9136
6140
239
876
917
677
5900
4300
3400
4533
233
266
315
272
23418
22333
19317
21689
20G73-107times1305
1217
1507
2832
1852
1777
5189
1114
960
38
289
588
1197
691
6733
5000
4233
5322
286
210
312
269
2078
81992
817067
19261
21G73-107times(77371
timesBS
ECTCP
-1)
18551946
2730
2177
1801
3482
10415
5233
239
277
753
423
5500
4467
3433
4467243
197
273
238
23817
20263
17425
20502
22G73-107times
96AC
-93
1718
1840
28612140
1345
9333
14669
8449
297
845
1278
807
5600
5033
4633
5089
270
243
300
271
28537
21461
16875
22291
23G73-107timesTo
go-II
168018083087
2192
1112
4678
9319
5036
186
473
985
548
6033
4767
4200
5000
210
209
231
217
24864
24609
21625
23699
24G73-107times99HS-18
1398
1675
3370
2148
2753
8672
11918
7781
362
691
981
678
566746
00
3500
4589
170
247
270
229
24677
20567
17855
21033
2577245times
CVJ-2-5-3-1-3
1500
1728
2203
1810
2282
4679
11492
6151
328
424
1116
623
6000
4333
420048
44300
231
370
300
21257
20497
1772
519826
2677245times1305
143815772080
1698
949
10439
12053
7814
121
915
992
676
5767
4800
3500
4689
168246
316
244
1978
91779
015517
17699
2777245times(77371times
BSEC
TCP
-1)
150516972846
2016
3222
8518
12621
8120
469
827
1141
812
5400
4367
3833
4533
196
225
242
221
2092
819485
1719
219202
2877245times96AC
-93
1653
1797
2117
1856
1934
6056
11543
6511
352
563
911
608
5333
4267
3833
4478
186
169
198
184
22003
19054
1676
719275
2977245timesTo
go-II
1723
1718
3163
2201
1749
8250
15443
8481229
806
1412
816
5967
4700
4067
4911
174
245
298
239
22075
23503
19875
21818
3077245times99HS-18
2016
1970
28542280
3217
13027
3531
6591395
965
285
549
5733
4467
3833
4678
148
228
209
195
2212
721462
1690
020
163
31CV
J-2-5-3-1-3times
1305
1358
1422
2179
1653
1254
2803
1019
44750
178
254
801
411
6033
4467
3367
4622
312
193
330
278
22269
19547
17250
19689
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
1335
1742
2400
1826
922
5226
12806
6318
236
458
1054
583
5633
4433
3600
4556
321
315
259
298
2210
419524
17087
19572
33CV
J-2-5-3-1-3times
96AC
-93
1552
16803260
2164
166710094
11941
7901250
974
1115
779
5533
4533
446748
44
170
202
317
230
2392
123317
19333
2219
0
34CV
J-2-5-3-1-3times
Togo-II
142216072638
1889
1012
4163
11061
5412
261
377
891
510
6167
4967
4533
5222
207
225
325
252
23590
22860
20278
22243
35CV
J-2-5-3-1-3times
99HS-18
1435
1600
2700
1912
3056
3237
7089
4461449
301
658
469
5700
44673800
4656
148
178
225
183
22444
20240
1696
719884
361305times(77371times
BSEC
TCP
-1)
1499
1767
2058
1774
1882
4665
7920
4822
356
426
724
502
566742673800
4578
271
242
305
273
22011
19295
16417
19241
371305times96AC
-93
16781605
2757
2014
1516
10037
11960
7838
311
11381284
911
560040
673400
4356
182
276
251
236
2396
519625
15800
1979
738
1305timesTo
go-II
1177
16802249
1702
902
4676
9388
4989
241
456
795
497
6533
5533
4733
5600
214
266
296
259
21894
20217
19222
20444
391305times99HS-18
1774
1767
2766
2102
2145
4628
10618
5797
301
447
991
580
5933
4500
3733
4722
128
142
180
150
2275
719413
16967
1971
2
40(77371times
BSEC
TCP
-1)times
96AC
-93
1748
1795
31712238
1537
12586
14379
9501
211
1104
1183
833
546744
00346744
44
127
201
226
185
23294
21089
17858
2074
7
10 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(
days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
41(77371times
BSEC
TCP
-1)times
Togo-II
19652007
2653
2208
2136
5601
9503
5747
315
635
1001
650
5533
4833
410048
22
213
241
286
247
22662
22094
17667
20808
42(77371times
BSEC
TCP
-1)times
99HS-18
15011687
2705
1964
1935
7253
11806
6998
314
704
1067
695
5467
4433
5133
5011
177
167
175
173
22305
19277
16392
19325
4396AC
-93timesTo
go-II1759
1618
2123
1834
1539
10347
12212
8033
325
907
1090
774
5900
450039674789
214
269
339
274
23252
21554
16875
20560
4496AC
-93times
99HS-18
1353
1618
2925
1966
3532
7223
11950
7569
440
654
1024
706
5600
4900
4533
5011
149
175
206
177
24494
20698
18111
2110
145
Togo-IItimes
99HS-18
1117
1278
2582
1659
3448
6764
11910
7374
477
767
1207
817
6000
5133
4333
5156
183
203
216
201
17683
15541
14333
15853
Mean
1513
1618
2492
1874
1656
5773
9854
5761
272
538
885
565
586846
534116
4879
218
233
268
240
21297
19547
16529
1912
4SE
101
073
098
mdash079
152
317
mdash014
014
028
mdash351
365
428
mdash036
039
046
mdash985
1173
1211
mdashCD
284
205
276
mdash221
427
890
mdash038
040
079
mdash12
513
015
2mdash
013
014
016
mdash351
418
431
mdash
(c)
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
1642
1667
1744
1684
1504
1349
1526
1460
2783
2405
2634
2607
836
745
908
830
4287
3754
4160
4067
2H7729-2
1693
1817
1950
1820
1012
859
1016
962
3433
2809
2591
2944
649
597
651
632
4445
3668
3607
3906
3G73-107
2000
2028
2077
2035
2280
1966
2312
2186
4695
4023
4229
4316
1281
1170
1463
1305
6975
5989
6541
6502
477245
1523
1630
1728
1627
1796
2489
2702
2329
5423
4851
4498
4924
748
1464
1743
1319
7219
7341
7200
7253
5CV
J-2-5-3-1-3
2282
2692
2787
2587
2411
2643
2901
2652
5857
4836
5024
5239
1206
1335
1769
1437
8268
7479
7925
7891
61305
1851
1951
2054
1952
2026
1763
1908
1899
4657
4414
4463
4511
1468
1336
1539
1448
6683
6177
6371
6410
777371timesBS
ECTCP
-11629
1645
1733
1669
1244
1692
1884
1607
4925
4183
4435
4515
625
1092
1483
1067
6169
5875
6319
6121
896AC
-93
1379
1519
1600
1499
2066
2942
3088
2698
4501
4095
4229
4275
984
1565
1678
1409
6567
7037
7316
6973
9To
go-II
1904
2057
2103
2021
2167
1844
2358
2123
4520
3446
3904
3957
1298
1138
1502
1313
6687
5290
6262
6080
1099HS-18
1475
1485
1633
1531
3180
2904
3382
3155
5633
4139
5205
4992
1656
1536
1879
1690
8813
7043
8587
8147
1H77833-2timesH7729-2
2010
2046
2133
2063
3645
3075
4377
3699
7675
6540
7199
7138
2014
1708
2880
2201
11320
9615
11576
10837
2H77833-2timesG73-107
2066
2101
2122
2096
5002
4053
4601
4552
7882
7430
6963
7425
3146
2565
2808
2840
12884
11482
11564
11977
3H77833-2times77245
1764
2048
2083
1965
3769
4497
4559
4275
8681
8515
8475
8557
2284
3356
3377
3006
12450
13012
13034
12832
4H77833-2times
CVJ-2-5-3-1-3
2205
2383
2733
2441
3693
5655
5220
4856
11656
10287
1010
510683
2198
4488
4176
3621
15348
1594
215325
15538
5H77833-2times1305
2209
2423
2491
2374
6008
5842
6933
6261
15238
14311
13889
14479
3758
4057
5591
4469
21246
2015
320822
2074
0
6H77833-2times(77371times
BSEC
TCP
-1)
1789
1823
1992
1868
3593
4288
4903
4262
8922
6522
7429
7625
2178
3224
3070
2824
12515
10811
12333
11886
7H77833-2times96AC
-93
1739
1960
2011
1903
4557
4350
4818
4575
7916
6068
7290
7092
2411
2529
3191
2710
12474
10419
1210
811667
8H77833-2timesTo
go-II
1927
2389
2406
2241
4185
5828
6215
5409
12319
10279
11023
11207
2433
4254
4631
3773
16504
1610
717238
16616
9H77833-2times99HS-18
1941
2245
2330
2172
4227
4139
4529
4298
9211
8120
7969
8433
3154
3184
3253
3197
13438
12259
12498
1273
210
H7729-2timesG73-107
1968
2109
2158
2079
3725
3042
3488
3418
7313
5956
6174
6481
2822
2001
2357
2393
11038
8998
9662
9899
Advances in Agriculture 11
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
11H7729-2times77245
1847
2048
2200
2032
2467
3759
5466
3898
7672
7449
8921
8014
1356
2350
3796
2500
1013
911208
14387
11911
12H7729-2times
CVJ-2-5-3-1-3
2218
2585
2767
2523
3674
4372
4483
4176
10053
9467
8664
9395
2417
3312
3345
3025
1372
813839
1314
713571
13H7729-2times1305
2169
2338
2547
2352
6728
6166
6792
6562
13333
11662
13301
1276
54128
4501
5660
4763
20061
17828
20093
19327
14H7729-2times(77371times
BSEC
TCP
-1)
1925
2095
2127
2049
3530
4165
4649
4115
9638
6917
7698
8084
1681
2833
3150
2555
1316
8110
82
12347
1219
915
H7729-2times96AC
-93
2012
2070
2180
2087
4955
4447
4596
4666
8738
6882
7762
7794
3754
3558
3181
3498
13693
11329
12359
12460
16H7729-2timesTo
go-II
2138
2428
2563
2377
4949
4660
5118
4909
8549
7243
7191
7661
3586
3353
3028
3322
13498
11903
12310
12570
17H7729-2times99HS-18
1899
1933
2073
1969
4046
4527
4988
4520
8492
7895
8144
8177
2561
3378
3553
3164
12539
12422
1313
212698
18G73-107times77245
1840
2253
2437
2177
2580
4776
5915
4424
11673
10608
10459
10913
1358
3163
4286
2936
14253
15385
16374
15337
19G73-107times
CVJ-2-5-3-1-3
2324
2537
2691
2517
4958
5030
5599
5196
9524
9395
1114
610022
3241
3645
4444
3776
14483
14425
1674
515217
20G73-107times1305
2228
2433
2583
2415
6340
5794
6373
6169
12490
1015
8119
61
11536
4199
4199
5018
4472
18830
1595
218334
1770
6
21G73-107times(77371times
BSEC
TCP
-1)
1943
2128
2220
2097
3936
3980
5770
4562
11697
9858
10303
10619
1874
2369
4181
2808
15633
13839
16073
1518
222
G73-107times96AC
-93
2118
2218
2317
2217
4270
5843
6201
5438
10319
9872
1015
910117
2703
4460
4379
3847
14589
15715
16359
15555
23G73-107timesTo
go-II
2237
2365
2733
2445
5736
5175
6822
5911
12098
10808
1299
611967
3984
3670
5372
4342
17835
1598
219818
17878
24G73-107times99HS-18
2025
2081
2322
2143
3558
4229
6612
4800
12350
9685
11516
1118
42196
2782
5009
3329
1590
813914
1812
915983
2577245timesCV
J-2-5-3-1-3
2109
2360
2675
2381
6760
6558
7299
6872
15225
11861
1416
21374
94225
4752
5839
4939
2198
518418
21461
20621
2677245times1305
1919
2100
2197
2072
5700
5142
5347
5397
11687
10565
10737
10996
4318
3956
4244
4173
17386
1570
816085
16393
2777245times(77371times
BSEC
TCP
-1)
1851
1935
2020
1936
2577
2460
2838
2625
6989
4778
4636
5468
1631
1662
1999
1764
9566
7238
7474
8093
2877245times96AC
-93
1729
1780
2133
1881
5541
5058
5748
5449
11952
8174
9526
9884
3646
3587
4071
3768
17493
13232
15274
15333
2977245timesTo
go-II
2300
2508
2658
2489
3067
5431
5963
4820
11737
11021
1216
411641
1598
4053
4770
3474
14805
16452
1812
616461
3077245times99HS-18
1982
2019
2223
2075
3846
3119
4225
3730
7493
6720
6476
6896
3205
2578
2643
2809
11339
9839
1070
110626
12 Advances in Agriculture
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
31CV
J-2-5-3-1-3times1305
2406
2553
3067
2675
4178
4796
6281
5085
13822
11425
12252
12500
2472
3241
4907
3540
18000
16222
18532
17585
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
2142
2173
2238
2184
4522
5235
5640
5132
11673
9658
10248
10526
2614
3766
4178
3519
1619
514893
15888
15659
33CV
J-2-5-3-1-3times
96AC
-93
2350
2477
2600
2476
4676
4280
6090
5015
11975
9832
10868
10891
3097
2931
4445
3491
16651
14111
1695
815907
34CV
J-2-5-3-1-3times
Togo-II
2093
2666
2989
2583
4058
3727
5190
4325
12588
10717
11217
11507
2742
2570
4185
3166
16645
14443
16407
15832
35CV
J-2-5-3-1-3times
99HS-18
2028
2311
2386
2242
3287
2513
5356
3719
10055
9733
1036
410051
2004
1581
4251
2612
13342
12246
1572
01376
9
361305times(77371times
BSEC
TCP
-1)
2005
2173
2210
2129
3569
4692
5140
4467
11652
9826
10041
10507
2005
3665
4179
3283
15221
14518
1518
214974
371305times96AC
-93
1822
2003
2047
1957
5115
4023
4813
4650
9965
7675
7571
8404
3456
2647
3155
3086
15080
11698
12384
13054
381305timesTo
go-II
2545
2583
2644
2591
6885
7283
6795
6987
12265
11410
12583
12086
4500
6120
5350
5323
1915
018693
19378
19074
391305times99HS-18
2070
2408
2593
2357
5219
4332
4800
4784
1092
97677
8968
9192
3924
3332
3840
3699
1614
912009
1376
913976
40(77371times
BSEC
TCP
-1)
times96AC
-93
2056
2115
2157
2109
3158
4463
4717
4113
9166
7540
8100
8268
1880
3542
3347
2923
12324
12003
12817
12381
41(77371times
BSEC
TCP
-1)
timesTo
go-II
1868
2253
2450
2190
4422
6458
7038
5973
1415
312369
1379
713439
2291
4646
5586
4174
18575
18827
20835
19412
42(77371times
BSEC
TCP
-1)
times99HS-18
1995
2026
2209
2077
2720
2909
3543
3057
7392
4692
5023
5702
1766
2187
2147
2033
10112
7601
8566
8760
4396AC
-93timesTo
go-II
1953
2027
2511
2164
5972
4391
7126
5830
15537
1392
714432
14632
3780
2538
5655
3991
21510
18318
21558
20462
4496AC
-93times99HS-18
1952
2021
2242
2072
3465
2758
4496
3573
5959
5186
6094
5746
2707
2154
2409
2423
9424
7944
10590
9319
45To
go-IItimes
99HS-18
1932
2008
2658
2199
5381
4995
5436
5270
10299
8876
9244
9473
3986
3842
3668
3832
15680
13871
14680
1474
3Mean
1982
2146
2301
2143
3963
4123
4836
4307
9498
8087
8663
8749
2510
2914
3513
2979
13460
12210
13499
13056
SE243
198
245
mdash671
501
626
mdash959
750
822
mdash423
349
344
mdash1155
927
1167
mdashCD
087
071
087
mdash239
178
223
mdash342
267
293
mdash15
112
412
2mdash
411
330
416
mdash
Advances in Agriculture 13Ta
ble4Analysis
ofvaria
ncefor
10times10
half-diallelfor
different
charactersin
threee
nviro
nments
(a)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Germination
()
Rateof
emergence
STI()
SSTI
()
Num
bero
fleavesseedling
Seedlin
gheight
(cm)
Freshweight
(g)seedlin
gDry
weight
(g)seedlin
g
E 1
Replicates
2652731
596768
783393
20700
93018674
33764
1033999
002590
Treatm
ents
541097
0255lowastlowast
8181153lowastlowast
30863092lowastlowast
20680900lowastlowast
1100708lowastlowast
2106317lowastlowast
930829527lowastlowast
319767lowastlowast
Parents
95118
163lowastlowast
14289925lowastlowast
59114816lowastlowast
18880254lowastlowast
1084227lowastlowast
1087906lowastlowast
22561719lowastlowast
042162lowastlowast
Hybrid
s44
11423573lowastlowast
3398933lowastlowast
25697199lowastlowast
20633716lowastlowast
472874lowastlowast
1391526lowastlowast
674123518lowastlowast
261839lowastlowast
Parentsv
ersush
ybrid
s1
43693104lowastlowast
163619
916lowastlowast
3896838
3896
2793lowastlowast
28873741lowastlowast
4272
2798lowastlowast
234144289lowastlowast
5367022lowastlowast
Error
108
281184
1356832
1173684
674337
040104
306
462
2015
1619
005535
E 2
Replicates
2583819
3707389lowast
1596635
198738
034925
448213
899529
007177
Treatm
ents
549997443lowastlowast
2825177lowastlowast
35378177lowastlowast
19074306lowastlowast
665284lowastlowast
1764
081lowastlowast
257694570lowastlowast
2177519lowastlowast
Parents
96507784lowastlowast
3718
879lowastlowast
69866289lowastlowast
1798
5404lowastlowast
705379lowastlowast
1408518lowastlowast
10612519lowastlowast
077429lowastlowast
Hybrid
s44
10569731lowastlowast
2127302lowastlowast
2910
2537lowastlowast
19429240lowastlowast
329256lowastlowast
7000
03lowastlowast
186026552lowastlowast
1483428lowastlowast
Parentsv
ersush
ybrid
s1
16223710lowastlowast
25488361lowastlowast
1113
326
13257362lowastlowast
15089673lowastlowast
5178
3569lowastlowast
5634825791lowastlowast
51618348lowastlowast
Error
108
334760
1097039
1049505
635570
06764
116
0576
694613
006206
E 3
Replicates
2894398
4808815lowast
mdashmdash
051076
095989
3768421
030220
Treatm
ents
541261560
0lowastlowast
4842216lowastlowast
mdashmdash
2811077lowastlowast
5947316lowastlowast
3870844
50lowastlowast
3445076lowastlowast
Parents
97135
735lowastlowast
1097
6388lowastlowast
mdashmdash
1468699lowastlowast
1112
764lowastlowast
136081650lowastlowast
1307836lowastlowast
Hybrid
s44
13217740lowastlowast
1379360
mdashmdash
15540
71lowastlowast
4581632lowastlowast
252084767lowastlowast
2378032lowastlowast
Parentsv
ersush
ybrid
s1
35440
180lowastlowast
102000335lowastlowast
mdashmdash
70200767lowastlowast
109548362lowastlowast
8586095705lowastlowast
69630188lowastlowast
Error
108
782907
1085444
mdashmdash
139771
291325
3019
169
02400
4(b)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Panicle
emergence
Effectiv
etillersplant
Planth
eight
Earlength
Earw
eightp
lant
Dry
fodd
eryieldplant
Grain
yieldplant
Totalbiological
yieldplant
E 1
Replicates
231860
61lowastlowast
416348lowastlowast
1672
9472lowast
1706
411077192
9634539
392777
115544
59Treatm
ents
544152
368lowastlowast
085657lowastlowast
326706089lowastlowast
1602572lowastlowast
6177
0406lowastlowast
304113
204lowastlowast
34285030lowastlowast
575617
582lowastlowast
Parents
94511481lowastlowast
018538lowastlowast
140807476lowastlowast
2240093lowastlowast
11653
959lowastlowast
27014155lowastlowast
3858650lowastlowast
61321919lowastlowast
Hybrid
s44
3201347lowastlowast
100797lowastlowast
110395657lowastlowast
1010
497lowastlowast
40296671lowastlowast
171271411lowastlowast
2413
6880lowastlowast
302999650lowastlowast
Parentsv
ersush
ybrid
s1
4276
5286lowastlowast
02360
9lowast11517452
616lowastlowast
2191
6167lowastlowast
1457662749lowastlowast
8643043524lowastlowast
7546
41053lowastlowast
17199467546lowastlowast
Error
108
468159
004907
3692099
224610
17114
303498126
682350
5075268
E 2
Replicates
2656364
002556
61575565lowastlowast
828842lowastlowast
589094
2268506
260877
1507116
Treatm
ents
542999618lowastlowast
069822lowastlowast
268998723lowastlowast
2436886lowastlowast
60592784lowastlowast
247259877lowastlowast
4192
0073lowastlowast
513483489lowastlowast
Parents
926300
00lowastlowast
048079lowastlowast
165555
405lowastlowast
3871874lowastlowast
1412
2393lowastlowast
19543682lowastlowast
3132
426lowastlowast
57230706lowastlowast
Hybrid
s44
2533
165lowastlowast
07344
9lowastlowast
9616
2857lowastlowast
1463359lowastlowast
35495366lowastlowast
154759288lowastlowast
26262465lowastlowast
293492304lowastlowast
Parentsv
ersush
ybrid
s1
26850135lowastlowast
105929lowastlowast
880476
6697lowastlowast
32357161lowastlowast
1583112746lowastlowast
6366731560lowastlowast
107994
3646lowastlowast
14299370699lowastlowast
Error
108
507598
005829
5234122
149406
955133
2142411
462311
3273886
E 3
Replicates
2029697
021161
9459847
143746
985141
46800
6492
6334
3345160
Treatm
ents
548045499lowastlowast
094511lowastlowast
192420108lowastlowast
3279225lowastlowast
72049211lowastlowast
298953
021lowastlowast
55061308lowastlowast
651732994lowastlowast
Parents
98010
741lowastlowast
094166lowastlowast
116037343lowastlowast
3702185lowastlowast
16305190lowastlowast
2323644
0lowastlowast
4553
893lowastlowast
71484982lowastlowast
Hybrid
s44
6254377lowastlowast
095514lowastlowast
7378
1513lowastlowast
2188348lowastlowast
31813193lowastlowast
190263916lowastlowast
31572179lowastlowast
368689934lowastlowast
Parentsv
ersush
ybrid
s1
8716
7710lowastlowast
053496lowast
609996
3199lowastlowast
47471212lowastlowast
2344
130203lowastlowast
756272
2861lowastlowast
154314
9707lowastlowast
18327859
719lowastlowast
Error
108
696981
007883
5578505
228608
1491014
2574
223
449588
5187705
lowast
Sign
ificant
at119875=005
lowastlowastSign
ificant
at119875=001
14 Advances in Agriculture
Table 5 Mean performance of the genotypes for membrane thermostability in two environments
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594
Advances in Agriculture 15
Table 5 Continued
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434
Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash
lowastValues are transformed values
0
6
12
18
24
30
36
42
48
54
minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity
G73-10796AC-93CVJ-2-5-3-1-3
Togo-II99HS-18
H77833-2H7729-2772451305
H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305
1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305
H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305
77245 times 1305
77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305
77371 times BSECT CP-1
H77833-2 times G73-107
Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits
moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling
survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The
16 Advances in Agriculture
Table6Po
oled
correla
tioncoeffi
cientamon
gvario
uscharactersin
threee
nviro
nments
Characters
Germination
Rateof
emergence
STI
SSTI
Leaves
seedlin
gSeedlin
gheight
Fresh
weight
Dry
weight
MTS
Panicle
emergence
Effectiv
etillersplant
Plant
height
Earlength
Ear
weightp
lant
Dry
fodd
eryield
plant
Grain
yield
plant
Total
biological
yield
plant
Germination
1000
0357lowastlowast
0353lowastlowast
0590lowastlowast
0230
0373lowastlowast
0293lowast
0260
0276lowastminus0135minus0478lowastlowast
0326lowast
minus0069minus0104
minus0190
minus0145
minus0167
Rateof
emergence
1000
0233
0351lowastlowast
0605lowastlowast
0585lowastlowast
0551lowastlowast
0524lowastlowast
0139minus0593lowastlowast
0032
0517lowastlowast
0217
0388lowastlowast
0361lowastlowast
0348lowastlowast
0377lowastlowast
STI
1000
0837lowastlowast
0241
0129
0081
004
80890lowastlowast
minus0215
minus0211
0030minus0007minus0043
minus0101
minus0111
minus0084
SSTI
1000
0233
0264
0183
0140
0745lowastlowast
minus0247minus0268lowast
0184minus0017minus006
6minus0129
minus0144
minus0112
Num
bero
fleaves
1000
0683lowastlowast
0633lowastlowast
060
4lowastlowast
0176minus0474lowastlowast
0109
0576lowastlowast
0496lowastlowast
064
1lowastlowast
0607lowastlowast
0630lowastlowast
0630lowastlowast
Seedlin
gheight
1000
0703lowastlowast
0663lowastlowast
0031minus0493lowastlowast
minus0115
0735lowastlowast
0360lowastlowast
0526lowastlowast
0520lowastlowast
0515lowastlowast
0532lowastlowast
Freshweight
1000
0957lowastlowast
minus0016
0425lowastlowast
minus0082
0661lowastlowast
0351lowastlowast
0581lowastlowast
0496lowastlowast
0576lowastlowast
0533lowastlowast
Dry
weight
1000minus0033minus0428lowastlowast
minus0016
0634lowastlowast
0354lowastlowast
0620lowastlowast
0509lowastlowast
0614lowastlowast
0554lowastlowast
MTS
1000minus040
minus0177minus0037
0015
minus0074
minus0138
minus0131
minus0120
Panicle
emergence
1000
minus0150minus0269lowast
0022minus0329lowast
minus0337lowast
minus0280lowast
minus0341lowast
Effectiv
etillersplant
1000
minus0012
0170
0366lowastlowast
040
5lowastlowast
0323lowast
0401lowastlowast
Planth
eight
1000
0650lowastlowast
0603lowastlowast
0596lowastlowast
0619lowastlowast
0610lowastlowast
Earlength
1000
0608lowastlowast
0671lowastlowast
064
8lowastlowast
066
4lowastlowast
Earw
eightp
lant
1000
0910lowastlowast
0984lowastlowast
0957lowastlowast
Dry
fodd
eryield
plant
1000
0907lowastlowast
0991lowastlowast
Grain
yield
plant
1000
0950lowastlowast
Totalbiological
yield
plant
1000
lowastSign
ificant
at119875=005lowastlowastSign
ificant
at119875=001
Advances in Agriculture 17
faster germination and early vigour in seedling could act asphysical indicators for heat tolerance
Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits
Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics
Conflict of Interests
The authors declare that there is no conflict of interestsregarding the publication of this paper
References
[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981
[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993
[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006
[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993
[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013
[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007
[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965
[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011
[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001
[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009
[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962
[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967
[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963
[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001
Submit your manuscripts athttpwwwhindawicom
Nutrition and Metabolism
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Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Food ScienceInternational Journal of
Agronomy
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
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International Journal of
Microbiology
The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom
Applied ampEnvironmentalSoil Science
Volume 2014
AgricultureAdvances in
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
BiodiversityInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
GenomicsInternational Journal of
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Plant GenomicsInternational Journal of
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Biotechnology Research International
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Forestry ResearchInternational Journal of
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Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
EcologyInternational Journal of
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Veterinary Medicine International
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Cell BiologyInternational Journal of
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Evolutionary BiologyInternational Journal of
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8 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
777371timesBS
ECT
CP-1
1377
1487
1892
1585
928
2660
3330
2306
150
258
301
236
5733
4533
4700
4989
242
220
293
252
1513
814550
1315
014279
896AC
-93
1303
1259
1852
1471
1056
1445
5972
2825
185
147
586
306
5500
4833
3833
4722
256
304
318
293
15577
14032
10000
13203
9To
go-II
1056
1220
2272
1516
924
1466
9113
3834
146
129
883
386
6167
4533
4600
5100
185
149
124
153
14578
12073
10033
12228
1099HS-18
1277
1075
1808
1387
1241
2833
7070
3715
228
226
615
356
6100
5000
4167
5089
243
173
324
247
12627
10355
1017
311052
1H77833-2times
H7729-2
1432
1498
2233
1721
1350
4405
8373
4709
159
499
782
480
5767
4433
3800
4667309
290
248
282
21010
18071
15847
18309
2H77833-2times
G73-107
1675
1635
2205
1838
1236
3211
5789
3412
134
313
547
331
5833
4767
3833
4811
262
295
236
265
21253
20449
15808
1917
0
3H77833-2times
77245
1577
1522
2263
1787
1058
6933
8719
5570
255
553
782
530
5267
4633
4600
4833
273
201
265
246
2074
119511
15208
18487
4H77833-2times
CVJ-2-5-3-1-3
1791
1757
2165
1904
20218030
5276
5109
354
779
477
537
5533
4233
3767
4511
165
330
351
282
21827
20072
17110
19670
5H77833-2times1305
1700
1842
28412128
1427
6228
9134
5596
570
579
813
654
5800
4267
3333
4467340
426
451
406
19816
19444
16037
18432
6H77833-2times
(77371times
BSEC
TCP
-1)
1453
1515
3120
2029
789
465510428
5291
211
408
858
492
5233
4167
3267
4222
203
283
267
251
20677
1913
816470
1876
2
7H77833-2times
96AC
-93
1835
1783
2427
2015
2532
6649
1610
684
29
350
650
1473
824
5467
4233
3700
4467243
239
256
246
22278
20434
15735
19482
8H77833-2times
Togo-II
1564
15632500
1876
1882
4772
11660
6105
296
469
972
579
5667
4533
440048
67
169
260
211
213
2412
82375
619389
22424
9H77833-2times
99HS-18
159318002049
1814
1571
480214024
6799
203
544
1547
765
6033
460040
0048
78
261
225
256
247
23466
21081
1793
320
827
10H7729-2times
G73-107
14951568
2075
1713
959
5157
8517
4877
218
382
666
422
6133
5067
4833
5344
300
273
239
271
23651
2191
717083
20884
11H7729-2times77245
1557
1537
2613
1902
1851
5756
5729
4445366
562
524
484
5633
4800
4633
5022
133
233
297
221
1979
219914
15250
18319
12H7729-2times
CVJ-2-5-3-1-3
18442033
28932257
1651
806114885
8199
264
597
1054
638
6333
4600
4233
5056
170
211
217
199
24629
23339
1970
022556
13H7729-2times1305
1325
1677309920
34
1756
724314048
7682
318
635
1232
728
630046
67
3633
4867298
261
361
307
21666
19371
17422
19486
14H7729-2times(77371
timesBS
ECTCP
-1)
1958
1887
3105
2317
1118
4675
14411
6734
242
457
1318
672
5900
4500
3800
4733
164
209
240
204
23661
1995
419250
20955
15H7729-2times
96AC
-93
1512
1717
2779
2002266844
08
11393
6156
356
500
1211
689
5633
4533
4200
4789
221
195
282
232
2194
921635
17213
20266
Advances in Agriculture 9
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
16H7729-2times
Togo-II
1519
1738
2159
1805
2437
9867
1616
294
89
361
961
1527
950
6167
4700
4200
5022
234
272
230
246
26338
2414
520553
23678
17H7729-2times
99HS-18
1938
1787
3151
2292
1574
6218
11318
6370
290
606
956
617
6067
4767
406749
67
172
210
199
194
24689
21854
1778
921444
18G73-107times77245
1856
1813
2210
1960
2435
8550
7828
6271338
633
612
528
5500
4633
440048
44
199
255
307
254
1993
619277
16095
18436
19G73-107times
CVJ-2-5-3-1-3
1517
1769
2407
1897
1556
7728
9136
6140
239
876
917
677
5900
4300
3400
4533
233
266
315
272
23418
22333
19317
21689
20G73-107times1305
1217
1507
2832
1852
1777
5189
1114
960
38
289
588
1197
691
6733
5000
4233
5322
286
210
312
269
2078
81992
817067
19261
21G73-107times(77371
timesBS
ECTCP
-1)
18551946
2730
2177
1801
3482
10415
5233
239
277
753
423
5500
4467
3433
4467243
197
273
238
23817
20263
17425
20502
22G73-107times
96AC
-93
1718
1840
28612140
1345
9333
14669
8449
297
845
1278
807
5600
5033
4633
5089
270
243
300
271
28537
21461
16875
22291
23G73-107timesTo
go-II
168018083087
2192
1112
4678
9319
5036
186
473
985
548
6033
4767
4200
5000
210
209
231
217
24864
24609
21625
23699
24G73-107times99HS-18
1398
1675
3370
2148
2753
8672
11918
7781
362
691
981
678
566746
00
3500
4589
170
247
270
229
24677
20567
17855
21033
2577245times
CVJ-2-5-3-1-3
1500
1728
2203
1810
2282
4679
11492
6151
328
424
1116
623
6000
4333
420048
44300
231
370
300
21257
20497
1772
519826
2677245times1305
143815772080
1698
949
10439
12053
7814
121
915
992
676
5767
4800
3500
4689
168246
316
244
1978
91779
015517
17699
2777245times(77371times
BSEC
TCP
-1)
150516972846
2016
3222
8518
12621
8120
469
827
1141
812
5400
4367
3833
4533
196
225
242
221
2092
819485
1719
219202
2877245times96AC
-93
1653
1797
2117
1856
1934
6056
11543
6511
352
563
911
608
5333
4267
3833
4478
186
169
198
184
22003
19054
1676
719275
2977245timesTo
go-II
1723
1718
3163
2201
1749
8250
15443
8481229
806
1412
816
5967
4700
4067
4911
174
245
298
239
22075
23503
19875
21818
3077245times99HS-18
2016
1970
28542280
3217
13027
3531
6591395
965
285
549
5733
4467
3833
4678
148
228
209
195
2212
721462
1690
020
163
31CV
J-2-5-3-1-3times
1305
1358
1422
2179
1653
1254
2803
1019
44750
178
254
801
411
6033
4467
3367
4622
312
193
330
278
22269
19547
17250
19689
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
1335
1742
2400
1826
922
5226
12806
6318
236
458
1054
583
5633
4433
3600
4556
321
315
259
298
2210
419524
17087
19572
33CV
J-2-5-3-1-3times
96AC
-93
1552
16803260
2164
166710094
11941
7901250
974
1115
779
5533
4533
446748
44
170
202
317
230
2392
123317
19333
2219
0
34CV
J-2-5-3-1-3times
Togo-II
142216072638
1889
1012
4163
11061
5412
261
377
891
510
6167
4967
4533
5222
207
225
325
252
23590
22860
20278
22243
35CV
J-2-5-3-1-3times
99HS-18
1435
1600
2700
1912
3056
3237
7089
4461449
301
658
469
5700
44673800
4656
148
178
225
183
22444
20240
1696
719884
361305times(77371times
BSEC
TCP
-1)
1499
1767
2058
1774
1882
4665
7920
4822
356
426
724
502
566742673800
4578
271
242
305
273
22011
19295
16417
19241
371305times96AC
-93
16781605
2757
2014
1516
10037
11960
7838
311
11381284
911
560040
673400
4356
182
276
251
236
2396
519625
15800
1979
738
1305timesTo
go-II
1177
16802249
1702
902
4676
9388
4989
241
456
795
497
6533
5533
4733
5600
214
266
296
259
21894
20217
19222
20444
391305times99HS-18
1774
1767
2766
2102
2145
4628
10618
5797
301
447
991
580
5933
4500
3733
4722
128
142
180
150
2275
719413
16967
1971
2
40(77371times
BSEC
TCP
-1)times
96AC
-93
1748
1795
31712238
1537
12586
14379
9501
211
1104
1183
833
546744
00346744
44
127
201
226
185
23294
21089
17858
2074
7
10 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(
days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
41(77371times
BSEC
TCP
-1)times
Togo-II
19652007
2653
2208
2136
5601
9503
5747
315
635
1001
650
5533
4833
410048
22
213
241
286
247
22662
22094
17667
20808
42(77371times
BSEC
TCP
-1)times
99HS-18
15011687
2705
1964
1935
7253
11806
6998
314
704
1067
695
5467
4433
5133
5011
177
167
175
173
22305
19277
16392
19325
4396AC
-93timesTo
go-II1759
1618
2123
1834
1539
10347
12212
8033
325
907
1090
774
5900
450039674789
214
269
339
274
23252
21554
16875
20560
4496AC
-93times
99HS-18
1353
1618
2925
1966
3532
7223
11950
7569
440
654
1024
706
5600
4900
4533
5011
149
175
206
177
24494
20698
18111
2110
145
Togo-IItimes
99HS-18
1117
1278
2582
1659
3448
6764
11910
7374
477
767
1207
817
6000
5133
4333
5156
183
203
216
201
17683
15541
14333
15853
Mean
1513
1618
2492
1874
1656
5773
9854
5761
272
538
885
565
586846
534116
4879
218
233
268
240
21297
19547
16529
1912
4SE
101
073
098
mdash079
152
317
mdash014
014
028
mdash351
365
428
mdash036
039
046
mdash985
1173
1211
mdashCD
284
205
276
mdash221
427
890
mdash038
040
079
mdash12
513
015
2mdash
013
014
016
mdash351
418
431
mdash
(c)
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
1642
1667
1744
1684
1504
1349
1526
1460
2783
2405
2634
2607
836
745
908
830
4287
3754
4160
4067
2H7729-2
1693
1817
1950
1820
1012
859
1016
962
3433
2809
2591
2944
649
597
651
632
4445
3668
3607
3906
3G73-107
2000
2028
2077
2035
2280
1966
2312
2186
4695
4023
4229
4316
1281
1170
1463
1305
6975
5989
6541
6502
477245
1523
1630
1728
1627
1796
2489
2702
2329
5423
4851
4498
4924
748
1464
1743
1319
7219
7341
7200
7253
5CV
J-2-5-3-1-3
2282
2692
2787
2587
2411
2643
2901
2652
5857
4836
5024
5239
1206
1335
1769
1437
8268
7479
7925
7891
61305
1851
1951
2054
1952
2026
1763
1908
1899
4657
4414
4463
4511
1468
1336
1539
1448
6683
6177
6371
6410
777371timesBS
ECTCP
-11629
1645
1733
1669
1244
1692
1884
1607
4925
4183
4435
4515
625
1092
1483
1067
6169
5875
6319
6121
896AC
-93
1379
1519
1600
1499
2066
2942
3088
2698
4501
4095
4229
4275
984
1565
1678
1409
6567
7037
7316
6973
9To
go-II
1904
2057
2103
2021
2167
1844
2358
2123
4520
3446
3904
3957
1298
1138
1502
1313
6687
5290
6262
6080
1099HS-18
1475
1485
1633
1531
3180
2904
3382
3155
5633
4139
5205
4992
1656
1536
1879
1690
8813
7043
8587
8147
1H77833-2timesH7729-2
2010
2046
2133
2063
3645
3075
4377
3699
7675
6540
7199
7138
2014
1708
2880
2201
11320
9615
11576
10837
2H77833-2timesG73-107
2066
2101
2122
2096
5002
4053
4601
4552
7882
7430
6963
7425
3146
2565
2808
2840
12884
11482
11564
11977
3H77833-2times77245
1764
2048
2083
1965
3769
4497
4559
4275
8681
8515
8475
8557
2284
3356
3377
3006
12450
13012
13034
12832
4H77833-2times
CVJ-2-5-3-1-3
2205
2383
2733
2441
3693
5655
5220
4856
11656
10287
1010
510683
2198
4488
4176
3621
15348
1594
215325
15538
5H77833-2times1305
2209
2423
2491
2374
6008
5842
6933
6261
15238
14311
13889
14479
3758
4057
5591
4469
21246
2015
320822
2074
0
6H77833-2times(77371times
BSEC
TCP
-1)
1789
1823
1992
1868
3593
4288
4903
4262
8922
6522
7429
7625
2178
3224
3070
2824
12515
10811
12333
11886
7H77833-2times96AC
-93
1739
1960
2011
1903
4557
4350
4818
4575
7916
6068
7290
7092
2411
2529
3191
2710
12474
10419
1210
811667
8H77833-2timesTo
go-II
1927
2389
2406
2241
4185
5828
6215
5409
12319
10279
11023
11207
2433
4254
4631
3773
16504
1610
717238
16616
9H77833-2times99HS-18
1941
2245
2330
2172
4227
4139
4529
4298
9211
8120
7969
8433
3154
3184
3253
3197
13438
12259
12498
1273
210
H7729-2timesG73-107
1968
2109
2158
2079
3725
3042
3488
3418
7313
5956
6174
6481
2822
2001
2357
2393
11038
8998
9662
9899
Advances in Agriculture 11
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
11H7729-2times77245
1847
2048
2200
2032
2467
3759
5466
3898
7672
7449
8921
8014
1356
2350
3796
2500
1013
911208
14387
11911
12H7729-2times
CVJ-2-5-3-1-3
2218
2585
2767
2523
3674
4372
4483
4176
10053
9467
8664
9395
2417
3312
3345
3025
1372
813839
1314
713571
13H7729-2times1305
2169
2338
2547
2352
6728
6166
6792
6562
13333
11662
13301
1276
54128
4501
5660
4763
20061
17828
20093
19327
14H7729-2times(77371times
BSEC
TCP
-1)
1925
2095
2127
2049
3530
4165
4649
4115
9638
6917
7698
8084
1681
2833
3150
2555
1316
8110
82
12347
1219
915
H7729-2times96AC
-93
2012
2070
2180
2087
4955
4447
4596
4666
8738
6882
7762
7794
3754
3558
3181
3498
13693
11329
12359
12460
16H7729-2timesTo
go-II
2138
2428
2563
2377
4949
4660
5118
4909
8549
7243
7191
7661
3586
3353
3028
3322
13498
11903
12310
12570
17H7729-2times99HS-18
1899
1933
2073
1969
4046
4527
4988
4520
8492
7895
8144
8177
2561
3378
3553
3164
12539
12422
1313
212698
18G73-107times77245
1840
2253
2437
2177
2580
4776
5915
4424
11673
10608
10459
10913
1358
3163
4286
2936
14253
15385
16374
15337
19G73-107times
CVJ-2-5-3-1-3
2324
2537
2691
2517
4958
5030
5599
5196
9524
9395
1114
610022
3241
3645
4444
3776
14483
14425
1674
515217
20G73-107times1305
2228
2433
2583
2415
6340
5794
6373
6169
12490
1015
8119
61
11536
4199
4199
5018
4472
18830
1595
218334
1770
6
21G73-107times(77371times
BSEC
TCP
-1)
1943
2128
2220
2097
3936
3980
5770
4562
11697
9858
10303
10619
1874
2369
4181
2808
15633
13839
16073
1518
222
G73-107times96AC
-93
2118
2218
2317
2217
4270
5843
6201
5438
10319
9872
1015
910117
2703
4460
4379
3847
14589
15715
16359
15555
23G73-107timesTo
go-II
2237
2365
2733
2445
5736
5175
6822
5911
12098
10808
1299
611967
3984
3670
5372
4342
17835
1598
219818
17878
24G73-107times99HS-18
2025
2081
2322
2143
3558
4229
6612
4800
12350
9685
11516
1118
42196
2782
5009
3329
1590
813914
1812
915983
2577245timesCV
J-2-5-3-1-3
2109
2360
2675
2381
6760
6558
7299
6872
15225
11861
1416
21374
94225
4752
5839
4939
2198
518418
21461
20621
2677245times1305
1919
2100
2197
2072
5700
5142
5347
5397
11687
10565
10737
10996
4318
3956
4244
4173
17386
1570
816085
16393
2777245times(77371times
BSEC
TCP
-1)
1851
1935
2020
1936
2577
2460
2838
2625
6989
4778
4636
5468
1631
1662
1999
1764
9566
7238
7474
8093
2877245times96AC
-93
1729
1780
2133
1881
5541
5058
5748
5449
11952
8174
9526
9884
3646
3587
4071
3768
17493
13232
15274
15333
2977245timesTo
go-II
2300
2508
2658
2489
3067
5431
5963
4820
11737
11021
1216
411641
1598
4053
4770
3474
14805
16452
1812
616461
3077245times99HS-18
1982
2019
2223
2075
3846
3119
4225
3730
7493
6720
6476
6896
3205
2578
2643
2809
11339
9839
1070
110626
12 Advances in Agriculture
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
31CV
J-2-5-3-1-3times1305
2406
2553
3067
2675
4178
4796
6281
5085
13822
11425
12252
12500
2472
3241
4907
3540
18000
16222
18532
17585
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
2142
2173
2238
2184
4522
5235
5640
5132
11673
9658
10248
10526
2614
3766
4178
3519
1619
514893
15888
15659
33CV
J-2-5-3-1-3times
96AC
-93
2350
2477
2600
2476
4676
4280
6090
5015
11975
9832
10868
10891
3097
2931
4445
3491
16651
14111
1695
815907
34CV
J-2-5-3-1-3times
Togo-II
2093
2666
2989
2583
4058
3727
5190
4325
12588
10717
11217
11507
2742
2570
4185
3166
16645
14443
16407
15832
35CV
J-2-5-3-1-3times
99HS-18
2028
2311
2386
2242
3287
2513
5356
3719
10055
9733
1036
410051
2004
1581
4251
2612
13342
12246
1572
01376
9
361305times(77371times
BSEC
TCP
-1)
2005
2173
2210
2129
3569
4692
5140
4467
11652
9826
10041
10507
2005
3665
4179
3283
15221
14518
1518
214974
371305times96AC
-93
1822
2003
2047
1957
5115
4023
4813
4650
9965
7675
7571
8404
3456
2647
3155
3086
15080
11698
12384
13054
381305timesTo
go-II
2545
2583
2644
2591
6885
7283
6795
6987
12265
11410
12583
12086
4500
6120
5350
5323
1915
018693
19378
19074
391305times99HS-18
2070
2408
2593
2357
5219
4332
4800
4784
1092
97677
8968
9192
3924
3332
3840
3699
1614
912009
1376
913976
40(77371times
BSEC
TCP
-1)
times96AC
-93
2056
2115
2157
2109
3158
4463
4717
4113
9166
7540
8100
8268
1880
3542
3347
2923
12324
12003
12817
12381
41(77371times
BSEC
TCP
-1)
timesTo
go-II
1868
2253
2450
2190
4422
6458
7038
5973
1415
312369
1379
713439
2291
4646
5586
4174
18575
18827
20835
19412
42(77371times
BSEC
TCP
-1)
times99HS-18
1995
2026
2209
2077
2720
2909
3543
3057
7392
4692
5023
5702
1766
2187
2147
2033
10112
7601
8566
8760
4396AC
-93timesTo
go-II
1953
2027
2511
2164
5972
4391
7126
5830
15537
1392
714432
14632
3780
2538
5655
3991
21510
18318
21558
20462
4496AC
-93times99HS-18
1952
2021
2242
2072
3465
2758
4496
3573
5959
5186
6094
5746
2707
2154
2409
2423
9424
7944
10590
9319
45To
go-IItimes
99HS-18
1932
2008
2658
2199
5381
4995
5436
5270
10299
8876
9244
9473
3986
3842
3668
3832
15680
13871
14680
1474
3Mean
1982
2146
2301
2143
3963
4123
4836
4307
9498
8087
8663
8749
2510
2914
3513
2979
13460
12210
13499
13056
SE243
198
245
mdash671
501
626
mdash959
750
822
mdash423
349
344
mdash1155
927
1167
mdashCD
087
071
087
mdash239
178
223
mdash342
267
293
mdash15
112
412
2mdash
411
330
416
mdash
Advances in Agriculture 13Ta
ble4Analysis
ofvaria
ncefor
10times10
half-diallelfor
different
charactersin
threee
nviro
nments
(a)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Germination
()
Rateof
emergence
STI()
SSTI
()
Num
bero
fleavesseedling
Seedlin
gheight
(cm)
Freshweight
(g)seedlin
gDry
weight
(g)seedlin
g
E 1
Replicates
2652731
596768
783393
20700
93018674
33764
1033999
002590
Treatm
ents
541097
0255lowastlowast
8181153lowastlowast
30863092lowastlowast
20680900lowastlowast
1100708lowastlowast
2106317lowastlowast
930829527lowastlowast
319767lowastlowast
Parents
95118
163lowastlowast
14289925lowastlowast
59114816lowastlowast
18880254lowastlowast
1084227lowastlowast
1087906lowastlowast
22561719lowastlowast
042162lowastlowast
Hybrid
s44
11423573lowastlowast
3398933lowastlowast
25697199lowastlowast
20633716lowastlowast
472874lowastlowast
1391526lowastlowast
674123518lowastlowast
261839lowastlowast
Parentsv
ersush
ybrid
s1
43693104lowastlowast
163619
916lowastlowast
3896838
3896
2793lowastlowast
28873741lowastlowast
4272
2798lowastlowast
234144289lowastlowast
5367022lowastlowast
Error
108
281184
1356832
1173684
674337
040104
306
462
2015
1619
005535
E 2
Replicates
2583819
3707389lowast
1596635
198738
034925
448213
899529
007177
Treatm
ents
549997443lowastlowast
2825177lowastlowast
35378177lowastlowast
19074306lowastlowast
665284lowastlowast
1764
081lowastlowast
257694570lowastlowast
2177519lowastlowast
Parents
96507784lowastlowast
3718
879lowastlowast
69866289lowastlowast
1798
5404lowastlowast
705379lowastlowast
1408518lowastlowast
10612519lowastlowast
077429lowastlowast
Hybrid
s44
10569731lowastlowast
2127302lowastlowast
2910
2537lowastlowast
19429240lowastlowast
329256lowastlowast
7000
03lowastlowast
186026552lowastlowast
1483428lowastlowast
Parentsv
ersush
ybrid
s1
16223710lowastlowast
25488361lowastlowast
1113
326
13257362lowastlowast
15089673lowastlowast
5178
3569lowastlowast
5634825791lowastlowast
51618348lowastlowast
Error
108
334760
1097039
1049505
635570
06764
116
0576
694613
006206
E 3
Replicates
2894398
4808815lowast
mdashmdash
051076
095989
3768421
030220
Treatm
ents
541261560
0lowastlowast
4842216lowastlowast
mdashmdash
2811077lowastlowast
5947316lowastlowast
3870844
50lowastlowast
3445076lowastlowast
Parents
97135
735lowastlowast
1097
6388lowastlowast
mdashmdash
1468699lowastlowast
1112
764lowastlowast
136081650lowastlowast
1307836lowastlowast
Hybrid
s44
13217740lowastlowast
1379360
mdashmdash
15540
71lowastlowast
4581632lowastlowast
252084767lowastlowast
2378032lowastlowast
Parentsv
ersush
ybrid
s1
35440
180lowastlowast
102000335lowastlowast
mdashmdash
70200767lowastlowast
109548362lowastlowast
8586095705lowastlowast
69630188lowastlowast
Error
108
782907
1085444
mdashmdash
139771
291325
3019
169
02400
4(b)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Panicle
emergence
Effectiv
etillersplant
Planth
eight
Earlength
Earw
eightp
lant
Dry
fodd
eryieldplant
Grain
yieldplant
Totalbiological
yieldplant
E 1
Replicates
231860
61lowastlowast
416348lowastlowast
1672
9472lowast
1706
411077192
9634539
392777
115544
59Treatm
ents
544152
368lowastlowast
085657lowastlowast
326706089lowastlowast
1602572lowastlowast
6177
0406lowastlowast
304113
204lowastlowast
34285030lowastlowast
575617
582lowastlowast
Parents
94511481lowastlowast
018538lowastlowast
140807476lowastlowast
2240093lowastlowast
11653
959lowastlowast
27014155lowastlowast
3858650lowastlowast
61321919lowastlowast
Hybrid
s44
3201347lowastlowast
100797lowastlowast
110395657lowastlowast
1010
497lowastlowast
40296671lowastlowast
171271411lowastlowast
2413
6880lowastlowast
302999650lowastlowast
Parentsv
ersush
ybrid
s1
4276
5286lowastlowast
02360
9lowast11517452
616lowastlowast
2191
6167lowastlowast
1457662749lowastlowast
8643043524lowastlowast
7546
41053lowastlowast
17199467546lowastlowast
Error
108
468159
004907
3692099
224610
17114
303498126
682350
5075268
E 2
Replicates
2656364
002556
61575565lowastlowast
828842lowastlowast
589094
2268506
260877
1507116
Treatm
ents
542999618lowastlowast
069822lowastlowast
268998723lowastlowast
2436886lowastlowast
60592784lowastlowast
247259877lowastlowast
4192
0073lowastlowast
513483489lowastlowast
Parents
926300
00lowastlowast
048079lowastlowast
165555
405lowastlowast
3871874lowastlowast
1412
2393lowastlowast
19543682lowastlowast
3132
426lowastlowast
57230706lowastlowast
Hybrid
s44
2533
165lowastlowast
07344
9lowastlowast
9616
2857lowastlowast
1463359lowastlowast
35495366lowastlowast
154759288lowastlowast
26262465lowastlowast
293492304lowastlowast
Parentsv
ersush
ybrid
s1
26850135lowastlowast
105929lowastlowast
880476
6697lowastlowast
32357161lowastlowast
1583112746lowastlowast
6366731560lowastlowast
107994
3646lowastlowast
14299370699lowastlowast
Error
108
507598
005829
5234122
149406
955133
2142411
462311
3273886
E 3
Replicates
2029697
021161
9459847
143746
985141
46800
6492
6334
3345160
Treatm
ents
548045499lowastlowast
094511lowastlowast
192420108lowastlowast
3279225lowastlowast
72049211lowastlowast
298953
021lowastlowast
55061308lowastlowast
651732994lowastlowast
Parents
98010
741lowastlowast
094166lowastlowast
116037343lowastlowast
3702185lowastlowast
16305190lowastlowast
2323644
0lowastlowast
4553
893lowastlowast
71484982lowastlowast
Hybrid
s44
6254377lowastlowast
095514lowastlowast
7378
1513lowastlowast
2188348lowastlowast
31813193lowastlowast
190263916lowastlowast
31572179lowastlowast
368689934lowastlowast
Parentsv
ersush
ybrid
s1
8716
7710lowastlowast
053496lowast
609996
3199lowastlowast
47471212lowastlowast
2344
130203lowastlowast
756272
2861lowastlowast
154314
9707lowastlowast
18327859
719lowastlowast
Error
108
696981
007883
5578505
228608
1491014
2574
223
449588
5187705
lowast
Sign
ificant
at119875=005
lowastlowastSign
ificant
at119875=001
14 Advances in Agriculture
Table 5 Mean performance of the genotypes for membrane thermostability in two environments
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594
Advances in Agriculture 15
Table 5 Continued
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434
Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash
lowastValues are transformed values
0
6
12
18
24
30
36
42
48
54
minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity
G73-10796AC-93CVJ-2-5-3-1-3
Togo-II99HS-18
H77833-2H7729-2772451305
H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305
1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305
H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305
77245 times 1305
77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305
77371 times BSECT CP-1
H77833-2 times G73-107
Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits
moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling
survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The
16 Advances in Agriculture
Table6Po
oled
correla
tioncoeffi
cientamon
gvario
uscharactersin
threee
nviro
nments
Characters
Germination
Rateof
emergence
STI
SSTI
Leaves
seedlin
gSeedlin
gheight
Fresh
weight
Dry
weight
MTS
Panicle
emergence
Effectiv
etillersplant
Plant
height
Earlength
Ear
weightp
lant
Dry
fodd
eryield
plant
Grain
yield
plant
Total
biological
yield
plant
Germination
1000
0357lowastlowast
0353lowastlowast
0590lowastlowast
0230
0373lowastlowast
0293lowast
0260
0276lowastminus0135minus0478lowastlowast
0326lowast
minus0069minus0104
minus0190
minus0145
minus0167
Rateof
emergence
1000
0233
0351lowastlowast
0605lowastlowast
0585lowastlowast
0551lowastlowast
0524lowastlowast
0139minus0593lowastlowast
0032
0517lowastlowast
0217
0388lowastlowast
0361lowastlowast
0348lowastlowast
0377lowastlowast
STI
1000
0837lowastlowast
0241
0129
0081
004
80890lowastlowast
minus0215
minus0211
0030minus0007minus0043
minus0101
minus0111
minus0084
SSTI
1000
0233
0264
0183
0140
0745lowastlowast
minus0247minus0268lowast
0184minus0017minus006
6minus0129
minus0144
minus0112
Num
bero
fleaves
1000
0683lowastlowast
0633lowastlowast
060
4lowastlowast
0176minus0474lowastlowast
0109
0576lowastlowast
0496lowastlowast
064
1lowastlowast
0607lowastlowast
0630lowastlowast
0630lowastlowast
Seedlin
gheight
1000
0703lowastlowast
0663lowastlowast
0031minus0493lowastlowast
minus0115
0735lowastlowast
0360lowastlowast
0526lowastlowast
0520lowastlowast
0515lowastlowast
0532lowastlowast
Freshweight
1000
0957lowastlowast
minus0016
0425lowastlowast
minus0082
0661lowastlowast
0351lowastlowast
0581lowastlowast
0496lowastlowast
0576lowastlowast
0533lowastlowast
Dry
weight
1000minus0033minus0428lowastlowast
minus0016
0634lowastlowast
0354lowastlowast
0620lowastlowast
0509lowastlowast
0614lowastlowast
0554lowastlowast
MTS
1000minus040
minus0177minus0037
0015
minus0074
minus0138
minus0131
minus0120
Panicle
emergence
1000
minus0150minus0269lowast
0022minus0329lowast
minus0337lowast
minus0280lowast
minus0341lowast
Effectiv
etillersplant
1000
minus0012
0170
0366lowastlowast
040
5lowastlowast
0323lowast
0401lowastlowast
Planth
eight
1000
0650lowastlowast
0603lowastlowast
0596lowastlowast
0619lowastlowast
0610lowastlowast
Earlength
1000
0608lowastlowast
0671lowastlowast
064
8lowastlowast
066
4lowastlowast
Earw
eightp
lant
1000
0910lowastlowast
0984lowastlowast
0957lowastlowast
Dry
fodd
eryield
plant
1000
0907lowastlowast
0991lowastlowast
Grain
yield
plant
1000
0950lowastlowast
Totalbiological
yield
plant
1000
lowastSign
ificant
at119875=005lowastlowastSign
ificant
at119875=001
Advances in Agriculture 17
faster germination and early vigour in seedling could act asphysical indicators for heat tolerance
Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits
Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics
Conflict of Interests
The authors declare that there is no conflict of interestsregarding the publication of this paper
References
[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981
[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993
[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006
[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993
[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013
[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007
[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965
[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011
[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001
[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009
[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962
[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967
[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963
[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001
Submit your manuscripts athttpwwwhindawicom
Nutrition and Metabolism
Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Food ScienceInternational Journal of
Agronomy
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
Microbiology
The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom
Applied ampEnvironmentalSoil Science
Volume 2014
AgricultureAdvances in
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
BiodiversityInternational Journal of
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ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
GenomicsInternational Journal of
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Plant GenomicsInternational Journal of
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Biotechnology Research International
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Forestry ResearchInternational Journal of
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Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
EcologyInternational Journal of
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Veterinary Medicine International
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Cell BiologyInternational Journal of
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Evolutionary BiologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Advances in Agriculture 9
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
16H7729-2times
Togo-II
1519
1738
2159
1805
2437
9867
1616
294
89
361
961
1527
950
6167
4700
4200
5022
234
272
230
246
26338
2414
520553
23678
17H7729-2times
99HS-18
1938
1787
3151
2292
1574
6218
11318
6370
290
606
956
617
6067
4767
406749
67
172
210
199
194
24689
21854
1778
921444
18G73-107times77245
1856
1813
2210
1960
2435
8550
7828
6271338
633
612
528
5500
4633
440048
44
199
255
307
254
1993
619277
16095
18436
19G73-107times
CVJ-2-5-3-1-3
1517
1769
2407
1897
1556
7728
9136
6140
239
876
917
677
5900
4300
3400
4533
233
266
315
272
23418
22333
19317
21689
20G73-107times1305
1217
1507
2832
1852
1777
5189
1114
960
38
289
588
1197
691
6733
5000
4233
5322
286
210
312
269
2078
81992
817067
19261
21G73-107times(77371
timesBS
ECTCP
-1)
18551946
2730
2177
1801
3482
10415
5233
239
277
753
423
5500
4467
3433
4467243
197
273
238
23817
20263
17425
20502
22G73-107times
96AC
-93
1718
1840
28612140
1345
9333
14669
8449
297
845
1278
807
5600
5033
4633
5089
270
243
300
271
28537
21461
16875
22291
23G73-107timesTo
go-II
168018083087
2192
1112
4678
9319
5036
186
473
985
548
6033
4767
4200
5000
210
209
231
217
24864
24609
21625
23699
24G73-107times99HS-18
1398
1675
3370
2148
2753
8672
11918
7781
362
691
981
678
566746
00
3500
4589
170
247
270
229
24677
20567
17855
21033
2577245times
CVJ-2-5-3-1-3
1500
1728
2203
1810
2282
4679
11492
6151
328
424
1116
623
6000
4333
420048
44300
231
370
300
21257
20497
1772
519826
2677245times1305
143815772080
1698
949
10439
12053
7814
121
915
992
676
5767
4800
3500
4689
168246
316
244
1978
91779
015517
17699
2777245times(77371times
BSEC
TCP
-1)
150516972846
2016
3222
8518
12621
8120
469
827
1141
812
5400
4367
3833
4533
196
225
242
221
2092
819485
1719
219202
2877245times96AC
-93
1653
1797
2117
1856
1934
6056
11543
6511
352
563
911
608
5333
4267
3833
4478
186
169
198
184
22003
19054
1676
719275
2977245timesTo
go-II
1723
1718
3163
2201
1749
8250
15443
8481229
806
1412
816
5967
4700
4067
4911
174
245
298
239
22075
23503
19875
21818
3077245times99HS-18
2016
1970
28542280
3217
13027
3531
6591395
965
285
549
5733
4467
3833
4678
148
228
209
195
2212
721462
1690
020
163
31CV
J-2-5-3-1-3times
1305
1358
1422
2179
1653
1254
2803
1019
44750
178
254
801
411
6033
4467
3367
4622
312
193
330
278
22269
19547
17250
19689
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
1335
1742
2400
1826
922
5226
12806
6318
236
458
1054
583
5633
4433
3600
4556
321
315
259
298
2210
419524
17087
19572
33CV
J-2-5-3-1-3times
96AC
-93
1552
16803260
2164
166710094
11941
7901250
974
1115
779
5533
4533
446748
44
170
202
317
230
2392
123317
19333
2219
0
34CV
J-2-5-3-1-3times
Togo-II
142216072638
1889
1012
4163
11061
5412
261
377
891
510
6167
4967
4533
5222
207
225
325
252
23590
22860
20278
22243
35CV
J-2-5-3-1-3times
99HS-18
1435
1600
2700
1912
3056
3237
7089
4461449
301
658
469
5700
44673800
4656
148
178
225
183
22444
20240
1696
719884
361305times(77371times
BSEC
TCP
-1)
1499
1767
2058
1774
1882
4665
7920
4822
356
426
724
502
566742673800
4578
271
242
305
273
22011
19295
16417
19241
371305times96AC
-93
16781605
2757
2014
1516
10037
11960
7838
311
11381284
911
560040
673400
4356
182
276
251
236
2396
519625
15800
1979
738
1305timesTo
go-II
1177
16802249
1702
902
4676
9388
4989
241
456
795
497
6533
5533
4733
5600
214
266
296
259
21894
20217
19222
20444
391305times99HS-18
1774
1767
2766
2102
2145
4628
10618
5797
301
447
991
580
5933
4500
3733
4722
128
142
180
150
2275
719413
16967
1971
2
40(77371times
BSEC
TCP
-1)times
96AC
-93
1748
1795
31712238
1537
12586
14379
9501
211
1104
1183
833
546744
00346744
44
127
201
226
185
23294
21089
17858
2074
7
10 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(
days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
41(77371times
BSEC
TCP
-1)times
Togo-II
19652007
2653
2208
2136
5601
9503
5747
315
635
1001
650
5533
4833
410048
22
213
241
286
247
22662
22094
17667
20808
42(77371times
BSEC
TCP
-1)times
99HS-18
15011687
2705
1964
1935
7253
11806
6998
314
704
1067
695
5467
4433
5133
5011
177
167
175
173
22305
19277
16392
19325
4396AC
-93timesTo
go-II1759
1618
2123
1834
1539
10347
12212
8033
325
907
1090
774
5900
450039674789
214
269
339
274
23252
21554
16875
20560
4496AC
-93times
99HS-18
1353
1618
2925
1966
3532
7223
11950
7569
440
654
1024
706
5600
4900
4533
5011
149
175
206
177
24494
20698
18111
2110
145
Togo-IItimes
99HS-18
1117
1278
2582
1659
3448
6764
11910
7374
477
767
1207
817
6000
5133
4333
5156
183
203
216
201
17683
15541
14333
15853
Mean
1513
1618
2492
1874
1656
5773
9854
5761
272
538
885
565
586846
534116
4879
218
233
268
240
21297
19547
16529
1912
4SE
101
073
098
mdash079
152
317
mdash014
014
028
mdash351
365
428
mdash036
039
046
mdash985
1173
1211
mdashCD
284
205
276
mdash221
427
890
mdash038
040
079
mdash12
513
015
2mdash
013
014
016
mdash351
418
431
mdash
(c)
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
1642
1667
1744
1684
1504
1349
1526
1460
2783
2405
2634
2607
836
745
908
830
4287
3754
4160
4067
2H7729-2
1693
1817
1950
1820
1012
859
1016
962
3433
2809
2591
2944
649
597
651
632
4445
3668
3607
3906
3G73-107
2000
2028
2077
2035
2280
1966
2312
2186
4695
4023
4229
4316
1281
1170
1463
1305
6975
5989
6541
6502
477245
1523
1630
1728
1627
1796
2489
2702
2329
5423
4851
4498
4924
748
1464
1743
1319
7219
7341
7200
7253
5CV
J-2-5-3-1-3
2282
2692
2787
2587
2411
2643
2901
2652
5857
4836
5024
5239
1206
1335
1769
1437
8268
7479
7925
7891
61305
1851
1951
2054
1952
2026
1763
1908
1899
4657
4414
4463
4511
1468
1336
1539
1448
6683
6177
6371
6410
777371timesBS
ECTCP
-11629
1645
1733
1669
1244
1692
1884
1607
4925
4183
4435
4515
625
1092
1483
1067
6169
5875
6319
6121
896AC
-93
1379
1519
1600
1499
2066
2942
3088
2698
4501
4095
4229
4275
984
1565
1678
1409
6567
7037
7316
6973
9To
go-II
1904
2057
2103
2021
2167
1844
2358
2123
4520
3446
3904
3957
1298
1138
1502
1313
6687
5290
6262
6080
1099HS-18
1475
1485
1633
1531
3180
2904
3382
3155
5633
4139
5205
4992
1656
1536
1879
1690
8813
7043
8587
8147
1H77833-2timesH7729-2
2010
2046
2133
2063
3645
3075
4377
3699
7675
6540
7199
7138
2014
1708
2880
2201
11320
9615
11576
10837
2H77833-2timesG73-107
2066
2101
2122
2096
5002
4053
4601
4552
7882
7430
6963
7425
3146
2565
2808
2840
12884
11482
11564
11977
3H77833-2times77245
1764
2048
2083
1965
3769
4497
4559
4275
8681
8515
8475
8557
2284
3356
3377
3006
12450
13012
13034
12832
4H77833-2times
CVJ-2-5-3-1-3
2205
2383
2733
2441
3693
5655
5220
4856
11656
10287
1010
510683
2198
4488
4176
3621
15348
1594
215325
15538
5H77833-2times1305
2209
2423
2491
2374
6008
5842
6933
6261
15238
14311
13889
14479
3758
4057
5591
4469
21246
2015
320822
2074
0
6H77833-2times(77371times
BSEC
TCP
-1)
1789
1823
1992
1868
3593
4288
4903
4262
8922
6522
7429
7625
2178
3224
3070
2824
12515
10811
12333
11886
7H77833-2times96AC
-93
1739
1960
2011
1903
4557
4350
4818
4575
7916
6068
7290
7092
2411
2529
3191
2710
12474
10419
1210
811667
8H77833-2timesTo
go-II
1927
2389
2406
2241
4185
5828
6215
5409
12319
10279
11023
11207
2433
4254
4631
3773
16504
1610
717238
16616
9H77833-2times99HS-18
1941
2245
2330
2172
4227
4139
4529
4298
9211
8120
7969
8433
3154
3184
3253
3197
13438
12259
12498
1273
210
H7729-2timesG73-107
1968
2109
2158
2079
3725
3042
3488
3418
7313
5956
6174
6481
2822
2001
2357
2393
11038
8998
9662
9899
Advances in Agriculture 11
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
11H7729-2times77245
1847
2048
2200
2032
2467
3759
5466
3898
7672
7449
8921
8014
1356
2350
3796
2500
1013
911208
14387
11911
12H7729-2times
CVJ-2-5-3-1-3
2218
2585
2767
2523
3674
4372
4483
4176
10053
9467
8664
9395
2417
3312
3345
3025
1372
813839
1314
713571
13H7729-2times1305
2169
2338
2547
2352
6728
6166
6792
6562
13333
11662
13301
1276
54128
4501
5660
4763
20061
17828
20093
19327
14H7729-2times(77371times
BSEC
TCP
-1)
1925
2095
2127
2049
3530
4165
4649
4115
9638
6917
7698
8084
1681
2833
3150
2555
1316
8110
82
12347
1219
915
H7729-2times96AC
-93
2012
2070
2180
2087
4955
4447
4596
4666
8738
6882
7762
7794
3754
3558
3181
3498
13693
11329
12359
12460
16H7729-2timesTo
go-II
2138
2428
2563
2377
4949
4660
5118
4909
8549
7243
7191
7661
3586
3353
3028
3322
13498
11903
12310
12570
17H7729-2times99HS-18
1899
1933
2073
1969
4046
4527
4988
4520
8492
7895
8144
8177
2561
3378
3553
3164
12539
12422
1313
212698
18G73-107times77245
1840
2253
2437
2177
2580
4776
5915
4424
11673
10608
10459
10913
1358
3163
4286
2936
14253
15385
16374
15337
19G73-107times
CVJ-2-5-3-1-3
2324
2537
2691
2517
4958
5030
5599
5196
9524
9395
1114
610022
3241
3645
4444
3776
14483
14425
1674
515217
20G73-107times1305
2228
2433
2583
2415
6340
5794
6373
6169
12490
1015
8119
61
11536
4199
4199
5018
4472
18830
1595
218334
1770
6
21G73-107times(77371times
BSEC
TCP
-1)
1943
2128
2220
2097
3936
3980
5770
4562
11697
9858
10303
10619
1874
2369
4181
2808
15633
13839
16073
1518
222
G73-107times96AC
-93
2118
2218
2317
2217
4270
5843
6201
5438
10319
9872
1015
910117
2703
4460
4379
3847
14589
15715
16359
15555
23G73-107timesTo
go-II
2237
2365
2733
2445
5736
5175
6822
5911
12098
10808
1299
611967
3984
3670
5372
4342
17835
1598
219818
17878
24G73-107times99HS-18
2025
2081
2322
2143
3558
4229
6612
4800
12350
9685
11516
1118
42196
2782
5009
3329
1590
813914
1812
915983
2577245timesCV
J-2-5-3-1-3
2109
2360
2675
2381
6760
6558
7299
6872
15225
11861
1416
21374
94225
4752
5839
4939
2198
518418
21461
20621
2677245times1305
1919
2100
2197
2072
5700
5142
5347
5397
11687
10565
10737
10996
4318
3956
4244
4173
17386
1570
816085
16393
2777245times(77371times
BSEC
TCP
-1)
1851
1935
2020
1936
2577
2460
2838
2625
6989
4778
4636
5468
1631
1662
1999
1764
9566
7238
7474
8093
2877245times96AC
-93
1729
1780
2133
1881
5541
5058
5748
5449
11952
8174
9526
9884
3646
3587
4071
3768
17493
13232
15274
15333
2977245timesTo
go-II
2300
2508
2658
2489
3067
5431
5963
4820
11737
11021
1216
411641
1598
4053
4770
3474
14805
16452
1812
616461
3077245times99HS-18
1982
2019
2223
2075
3846
3119
4225
3730
7493
6720
6476
6896
3205
2578
2643
2809
11339
9839
1070
110626
12 Advances in Agriculture
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
31CV
J-2-5-3-1-3times1305
2406
2553
3067
2675
4178
4796
6281
5085
13822
11425
12252
12500
2472
3241
4907
3540
18000
16222
18532
17585
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
2142
2173
2238
2184
4522
5235
5640
5132
11673
9658
10248
10526
2614
3766
4178
3519
1619
514893
15888
15659
33CV
J-2-5-3-1-3times
96AC
-93
2350
2477
2600
2476
4676
4280
6090
5015
11975
9832
10868
10891
3097
2931
4445
3491
16651
14111
1695
815907
34CV
J-2-5-3-1-3times
Togo-II
2093
2666
2989
2583
4058
3727
5190
4325
12588
10717
11217
11507
2742
2570
4185
3166
16645
14443
16407
15832
35CV
J-2-5-3-1-3times
99HS-18
2028
2311
2386
2242
3287
2513
5356
3719
10055
9733
1036
410051
2004
1581
4251
2612
13342
12246
1572
01376
9
361305times(77371times
BSEC
TCP
-1)
2005
2173
2210
2129
3569
4692
5140
4467
11652
9826
10041
10507
2005
3665
4179
3283
15221
14518
1518
214974
371305times96AC
-93
1822
2003
2047
1957
5115
4023
4813
4650
9965
7675
7571
8404
3456
2647
3155
3086
15080
11698
12384
13054
381305timesTo
go-II
2545
2583
2644
2591
6885
7283
6795
6987
12265
11410
12583
12086
4500
6120
5350
5323
1915
018693
19378
19074
391305times99HS-18
2070
2408
2593
2357
5219
4332
4800
4784
1092
97677
8968
9192
3924
3332
3840
3699
1614
912009
1376
913976
40(77371times
BSEC
TCP
-1)
times96AC
-93
2056
2115
2157
2109
3158
4463
4717
4113
9166
7540
8100
8268
1880
3542
3347
2923
12324
12003
12817
12381
41(77371times
BSEC
TCP
-1)
timesTo
go-II
1868
2253
2450
2190
4422
6458
7038
5973
1415
312369
1379
713439
2291
4646
5586
4174
18575
18827
20835
19412
42(77371times
BSEC
TCP
-1)
times99HS-18
1995
2026
2209
2077
2720
2909
3543
3057
7392
4692
5023
5702
1766
2187
2147
2033
10112
7601
8566
8760
4396AC
-93timesTo
go-II
1953
2027
2511
2164
5972
4391
7126
5830
15537
1392
714432
14632
3780
2538
5655
3991
21510
18318
21558
20462
4496AC
-93times99HS-18
1952
2021
2242
2072
3465
2758
4496
3573
5959
5186
6094
5746
2707
2154
2409
2423
9424
7944
10590
9319
45To
go-IItimes
99HS-18
1932
2008
2658
2199
5381
4995
5436
5270
10299
8876
9244
9473
3986
3842
3668
3832
15680
13871
14680
1474
3Mean
1982
2146
2301
2143
3963
4123
4836
4307
9498
8087
8663
8749
2510
2914
3513
2979
13460
12210
13499
13056
SE243
198
245
mdash671
501
626
mdash959
750
822
mdash423
349
344
mdash1155
927
1167
mdashCD
087
071
087
mdash239
178
223
mdash342
267
293
mdash15
112
412
2mdash
411
330
416
mdash
Advances in Agriculture 13Ta
ble4Analysis
ofvaria
ncefor
10times10
half-diallelfor
different
charactersin
threee
nviro
nments
(a)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Germination
()
Rateof
emergence
STI()
SSTI
()
Num
bero
fleavesseedling
Seedlin
gheight
(cm)
Freshweight
(g)seedlin
gDry
weight
(g)seedlin
g
E 1
Replicates
2652731
596768
783393
20700
93018674
33764
1033999
002590
Treatm
ents
541097
0255lowastlowast
8181153lowastlowast
30863092lowastlowast
20680900lowastlowast
1100708lowastlowast
2106317lowastlowast
930829527lowastlowast
319767lowastlowast
Parents
95118
163lowastlowast
14289925lowastlowast
59114816lowastlowast
18880254lowastlowast
1084227lowastlowast
1087906lowastlowast
22561719lowastlowast
042162lowastlowast
Hybrid
s44
11423573lowastlowast
3398933lowastlowast
25697199lowastlowast
20633716lowastlowast
472874lowastlowast
1391526lowastlowast
674123518lowastlowast
261839lowastlowast
Parentsv
ersush
ybrid
s1
43693104lowastlowast
163619
916lowastlowast
3896838
3896
2793lowastlowast
28873741lowastlowast
4272
2798lowastlowast
234144289lowastlowast
5367022lowastlowast
Error
108
281184
1356832
1173684
674337
040104
306
462
2015
1619
005535
E 2
Replicates
2583819
3707389lowast
1596635
198738
034925
448213
899529
007177
Treatm
ents
549997443lowastlowast
2825177lowastlowast
35378177lowastlowast
19074306lowastlowast
665284lowastlowast
1764
081lowastlowast
257694570lowastlowast
2177519lowastlowast
Parents
96507784lowastlowast
3718
879lowastlowast
69866289lowastlowast
1798
5404lowastlowast
705379lowastlowast
1408518lowastlowast
10612519lowastlowast
077429lowastlowast
Hybrid
s44
10569731lowastlowast
2127302lowastlowast
2910
2537lowastlowast
19429240lowastlowast
329256lowastlowast
7000
03lowastlowast
186026552lowastlowast
1483428lowastlowast
Parentsv
ersush
ybrid
s1
16223710lowastlowast
25488361lowastlowast
1113
326
13257362lowastlowast
15089673lowastlowast
5178
3569lowastlowast
5634825791lowastlowast
51618348lowastlowast
Error
108
334760
1097039
1049505
635570
06764
116
0576
694613
006206
E 3
Replicates
2894398
4808815lowast
mdashmdash
051076
095989
3768421
030220
Treatm
ents
541261560
0lowastlowast
4842216lowastlowast
mdashmdash
2811077lowastlowast
5947316lowastlowast
3870844
50lowastlowast
3445076lowastlowast
Parents
97135
735lowastlowast
1097
6388lowastlowast
mdashmdash
1468699lowastlowast
1112
764lowastlowast
136081650lowastlowast
1307836lowastlowast
Hybrid
s44
13217740lowastlowast
1379360
mdashmdash
15540
71lowastlowast
4581632lowastlowast
252084767lowastlowast
2378032lowastlowast
Parentsv
ersush
ybrid
s1
35440
180lowastlowast
102000335lowastlowast
mdashmdash
70200767lowastlowast
109548362lowastlowast
8586095705lowastlowast
69630188lowastlowast
Error
108
782907
1085444
mdashmdash
139771
291325
3019
169
02400
4(b)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Panicle
emergence
Effectiv
etillersplant
Planth
eight
Earlength
Earw
eightp
lant
Dry
fodd
eryieldplant
Grain
yieldplant
Totalbiological
yieldplant
E 1
Replicates
231860
61lowastlowast
416348lowastlowast
1672
9472lowast
1706
411077192
9634539
392777
115544
59Treatm
ents
544152
368lowastlowast
085657lowastlowast
326706089lowastlowast
1602572lowastlowast
6177
0406lowastlowast
304113
204lowastlowast
34285030lowastlowast
575617
582lowastlowast
Parents
94511481lowastlowast
018538lowastlowast
140807476lowastlowast
2240093lowastlowast
11653
959lowastlowast
27014155lowastlowast
3858650lowastlowast
61321919lowastlowast
Hybrid
s44
3201347lowastlowast
100797lowastlowast
110395657lowastlowast
1010
497lowastlowast
40296671lowastlowast
171271411lowastlowast
2413
6880lowastlowast
302999650lowastlowast
Parentsv
ersush
ybrid
s1
4276
5286lowastlowast
02360
9lowast11517452
616lowastlowast
2191
6167lowastlowast
1457662749lowastlowast
8643043524lowastlowast
7546
41053lowastlowast
17199467546lowastlowast
Error
108
468159
004907
3692099
224610
17114
303498126
682350
5075268
E 2
Replicates
2656364
002556
61575565lowastlowast
828842lowastlowast
589094
2268506
260877
1507116
Treatm
ents
542999618lowastlowast
069822lowastlowast
268998723lowastlowast
2436886lowastlowast
60592784lowastlowast
247259877lowastlowast
4192
0073lowastlowast
513483489lowastlowast
Parents
926300
00lowastlowast
048079lowastlowast
165555
405lowastlowast
3871874lowastlowast
1412
2393lowastlowast
19543682lowastlowast
3132
426lowastlowast
57230706lowastlowast
Hybrid
s44
2533
165lowastlowast
07344
9lowastlowast
9616
2857lowastlowast
1463359lowastlowast
35495366lowastlowast
154759288lowastlowast
26262465lowastlowast
293492304lowastlowast
Parentsv
ersush
ybrid
s1
26850135lowastlowast
105929lowastlowast
880476
6697lowastlowast
32357161lowastlowast
1583112746lowastlowast
6366731560lowastlowast
107994
3646lowastlowast
14299370699lowastlowast
Error
108
507598
005829
5234122
149406
955133
2142411
462311
3273886
E 3
Replicates
2029697
021161
9459847
143746
985141
46800
6492
6334
3345160
Treatm
ents
548045499lowastlowast
094511lowastlowast
192420108lowastlowast
3279225lowastlowast
72049211lowastlowast
298953
021lowastlowast
55061308lowastlowast
651732994lowastlowast
Parents
98010
741lowastlowast
094166lowastlowast
116037343lowastlowast
3702185lowastlowast
16305190lowastlowast
2323644
0lowastlowast
4553
893lowastlowast
71484982lowastlowast
Hybrid
s44
6254377lowastlowast
095514lowastlowast
7378
1513lowastlowast
2188348lowastlowast
31813193lowastlowast
190263916lowastlowast
31572179lowastlowast
368689934lowastlowast
Parentsv
ersush
ybrid
s1
8716
7710lowastlowast
053496lowast
609996
3199lowastlowast
47471212lowastlowast
2344
130203lowastlowast
756272
2861lowastlowast
154314
9707lowastlowast
18327859
719lowastlowast
Error
108
696981
007883
5578505
228608
1491014
2574
223
449588
5187705
lowast
Sign
ificant
at119875=005
lowastlowastSign
ificant
at119875=001
14 Advances in Agriculture
Table 5 Mean performance of the genotypes for membrane thermostability in two environments
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594
Advances in Agriculture 15
Table 5 Continued
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434
Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash
lowastValues are transformed values
0
6
12
18
24
30
36
42
48
54
minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity
G73-10796AC-93CVJ-2-5-3-1-3
Togo-II99HS-18
H77833-2H7729-2772451305
H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305
1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305
H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305
77245 times 1305
77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305
77371 times BSECT CP-1
H77833-2 times G73-107
Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits
moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling
survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The
16 Advances in Agriculture
Table6Po
oled
correla
tioncoeffi
cientamon
gvario
uscharactersin
threee
nviro
nments
Characters
Germination
Rateof
emergence
STI
SSTI
Leaves
seedlin
gSeedlin
gheight
Fresh
weight
Dry
weight
MTS
Panicle
emergence
Effectiv
etillersplant
Plant
height
Earlength
Ear
weightp
lant
Dry
fodd
eryield
plant
Grain
yield
plant
Total
biological
yield
plant
Germination
1000
0357lowastlowast
0353lowastlowast
0590lowastlowast
0230
0373lowastlowast
0293lowast
0260
0276lowastminus0135minus0478lowastlowast
0326lowast
minus0069minus0104
minus0190
minus0145
minus0167
Rateof
emergence
1000
0233
0351lowastlowast
0605lowastlowast
0585lowastlowast
0551lowastlowast
0524lowastlowast
0139minus0593lowastlowast
0032
0517lowastlowast
0217
0388lowastlowast
0361lowastlowast
0348lowastlowast
0377lowastlowast
STI
1000
0837lowastlowast
0241
0129
0081
004
80890lowastlowast
minus0215
minus0211
0030minus0007minus0043
minus0101
minus0111
minus0084
SSTI
1000
0233
0264
0183
0140
0745lowastlowast
minus0247minus0268lowast
0184minus0017minus006
6minus0129
minus0144
minus0112
Num
bero
fleaves
1000
0683lowastlowast
0633lowastlowast
060
4lowastlowast
0176minus0474lowastlowast
0109
0576lowastlowast
0496lowastlowast
064
1lowastlowast
0607lowastlowast
0630lowastlowast
0630lowastlowast
Seedlin
gheight
1000
0703lowastlowast
0663lowastlowast
0031minus0493lowastlowast
minus0115
0735lowastlowast
0360lowastlowast
0526lowastlowast
0520lowastlowast
0515lowastlowast
0532lowastlowast
Freshweight
1000
0957lowastlowast
minus0016
0425lowastlowast
minus0082
0661lowastlowast
0351lowastlowast
0581lowastlowast
0496lowastlowast
0576lowastlowast
0533lowastlowast
Dry
weight
1000minus0033minus0428lowastlowast
minus0016
0634lowastlowast
0354lowastlowast
0620lowastlowast
0509lowastlowast
0614lowastlowast
0554lowastlowast
MTS
1000minus040
minus0177minus0037
0015
minus0074
minus0138
minus0131
minus0120
Panicle
emergence
1000
minus0150minus0269lowast
0022minus0329lowast
minus0337lowast
minus0280lowast
minus0341lowast
Effectiv
etillersplant
1000
minus0012
0170
0366lowastlowast
040
5lowastlowast
0323lowast
0401lowastlowast
Planth
eight
1000
0650lowastlowast
0603lowastlowast
0596lowastlowast
0619lowastlowast
0610lowastlowast
Earlength
1000
0608lowastlowast
0671lowastlowast
064
8lowastlowast
066
4lowastlowast
Earw
eightp
lant
1000
0910lowastlowast
0984lowastlowast
0957lowastlowast
Dry
fodd
eryield
plant
1000
0907lowastlowast
0991lowastlowast
Grain
yield
plant
1000
0950lowastlowast
Totalbiological
yield
plant
1000
lowastSign
ificant
at119875=005lowastlowastSign
ificant
at119875=001
Advances in Agriculture 17
faster germination and early vigour in seedling could act asphysical indicators for heat tolerance
Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits
Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics
Conflict of Interests
The authors declare that there is no conflict of interestsregarding the publication of this paper
References
[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981
[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993
[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006
[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993
[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013
[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007
[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965
[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011
[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001
[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009
[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962
[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967
[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963
[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001
Submit your manuscripts athttpwwwhindawicom
Nutrition and Metabolism
Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Food ScienceInternational Journal of
Agronomy
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
Microbiology
The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom
Applied ampEnvironmentalSoil Science
Volume 2014
AgricultureAdvances in
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
BiodiversityInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
GenomicsInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Plant GenomicsInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Biotechnology Research International
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Forestry ResearchInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
EcologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Veterinary Medicine International
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Cell BiologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Evolutionary BiologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
10 Advances in Agriculture
(b)Con
tinued
Sr
NoGenotypes
Seedlin
gheight
(cm)
Freshweight(g)seedling
Dry
weight(g)seedling
Panicle
emergence(
days)
Effectiv
etillersplant
Planth
eight(cm
)E 1
E 2E 3
Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
41(77371times
BSEC
TCP
-1)times
Togo-II
19652007
2653
2208
2136
5601
9503
5747
315
635
1001
650
5533
4833
410048
22
213
241
286
247
22662
22094
17667
20808
42(77371times
BSEC
TCP
-1)times
99HS-18
15011687
2705
1964
1935
7253
11806
6998
314
704
1067
695
5467
4433
5133
5011
177
167
175
173
22305
19277
16392
19325
4396AC
-93timesTo
go-II1759
1618
2123
1834
1539
10347
12212
8033
325
907
1090
774
5900
450039674789
214
269
339
274
23252
21554
16875
20560
4496AC
-93times
99HS-18
1353
1618
2925
1966
3532
7223
11950
7569
440
654
1024
706
5600
4900
4533
5011
149
175
206
177
24494
20698
18111
2110
145
Togo-IItimes
99HS-18
1117
1278
2582
1659
3448
6764
11910
7374
477
767
1207
817
6000
5133
4333
5156
183
203
216
201
17683
15541
14333
15853
Mean
1513
1618
2492
1874
1656
5773
9854
5761
272
538
885
565
586846
534116
4879
218
233
268
240
21297
19547
16529
1912
4SE
101
073
098
mdash079
152
317
mdash014
014
028
mdash351
365
428
mdash036
039
046
mdash985
1173
1211
mdashCD
284
205
276
mdash221
427
890
mdash038
040
079
mdash12
513
015
2mdash
013
014
016
mdash351
418
431
mdash
(c)
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
1H77833-2
1642
1667
1744
1684
1504
1349
1526
1460
2783
2405
2634
2607
836
745
908
830
4287
3754
4160
4067
2H7729-2
1693
1817
1950
1820
1012
859
1016
962
3433
2809
2591
2944
649
597
651
632
4445
3668
3607
3906
3G73-107
2000
2028
2077
2035
2280
1966
2312
2186
4695
4023
4229
4316
1281
1170
1463
1305
6975
5989
6541
6502
477245
1523
1630
1728
1627
1796
2489
2702
2329
5423
4851
4498
4924
748
1464
1743
1319
7219
7341
7200
7253
5CV
J-2-5-3-1-3
2282
2692
2787
2587
2411
2643
2901
2652
5857
4836
5024
5239
1206
1335
1769
1437
8268
7479
7925
7891
61305
1851
1951
2054
1952
2026
1763
1908
1899
4657
4414
4463
4511
1468
1336
1539
1448
6683
6177
6371
6410
777371timesBS
ECTCP
-11629
1645
1733
1669
1244
1692
1884
1607
4925
4183
4435
4515
625
1092
1483
1067
6169
5875
6319
6121
896AC
-93
1379
1519
1600
1499
2066
2942
3088
2698
4501
4095
4229
4275
984
1565
1678
1409
6567
7037
7316
6973
9To
go-II
1904
2057
2103
2021
2167
1844
2358
2123
4520
3446
3904
3957
1298
1138
1502
1313
6687
5290
6262
6080
1099HS-18
1475
1485
1633
1531
3180
2904
3382
3155
5633
4139
5205
4992
1656
1536
1879
1690
8813
7043
8587
8147
1H77833-2timesH7729-2
2010
2046
2133
2063
3645
3075
4377
3699
7675
6540
7199
7138
2014
1708
2880
2201
11320
9615
11576
10837
2H77833-2timesG73-107
2066
2101
2122
2096
5002
4053
4601
4552
7882
7430
6963
7425
3146
2565
2808
2840
12884
11482
11564
11977
3H77833-2times77245
1764
2048
2083
1965
3769
4497
4559
4275
8681
8515
8475
8557
2284
3356
3377
3006
12450
13012
13034
12832
4H77833-2times
CVJ-2-5-3-1-3
2205
2383
2733
2441
3693
5655
5220
4856
11656
10287
1010
510683
2198
4488
4176
3621
15348
1594
215325
15538
5H77833-2times1305
2209
2423
2491
2374
6008
5842
6933
6261
15238
14311
13889
14479
3758
4057
5591
4469
21246
2015
320822
2074
0
6H77833-2times(77371times
BSEC
TCP
-1)
1789
1823
1992
1868
3593
4288
4903
4262
8922
6522
7429
7625
2178
3224
3070
2824
12515
10811
12333
11886
7H77833-2times96AC
-93
1739
1960
2011
1903
4557
4350
4818
4575
7916
6068
7290
7092
2411
2529
3191
2710
12474
10419
1210
811667
8H77833-2timesTo
go-II
1927
2389
2406
2241
4185
5828
6215
5409
12319
10279
11023
11207
2433
4254
4631
3773
16504
1610
717238
16616
9H77833-2times99HS-18
1941
2245
2330
2172
4227
4139
4529
4298
9211
8120
7969
8433
3154
3184
3253
3197
13438
12259
12498
1273
210
H7729-2timesG73-107
1968
2109
2158
2079
3725
3042
3488
3418
7313
5956
6174
6481
2822
2001
2357
2393
11038
8998
9662
9899
Advances in Agriculture 11
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
11H7729-2times77245
1847
2048
2200
2032
2467
3759
5466
3898
7672
7449
8921
8014
1356
2350
3796
2500
1013
911208
14387
11911
12H7729-2times
CVJ-2-5-3-1-3
2218
2585
2767
2523
3674
4372
4483
4176
10053
9467
8664
9395
2417
3312
3345
3025
1372
813839
1314
713571
13H7729-2times1305
2169
2338
2547
2352
6728
6166
6792
6562
13333
11662
13301
1276
54128
4501
5660
4763
20061
17828
20093
19327
14H7729-2times(77371times
BSEC
TCP
-1)
1925
2095
2127
2049
3530
4165
4649
4115
9638
6917
7698
8084
1681
2833
3150
2555
1316
8110
82
12347
1219
915
H7729-2times96AC
-93
2012
2070
2180
2087
4955
4447
4596
4666
8738
6882
7762
7794
3754
3558
3181
3498
13693
11329
12359
12460
16H7729-2timesTo
go-II
2138
2428
2563
2377
4949
4660
5118
4909
8549
7243
7191
7661
3586
3353
3028
3322
13498
11903
12310
12570
17H7729-2times99HS-18
1899
1933
2073
1969
4046
4527
4988
4520
8492
7895
8144
8177
2561
3378
3553
3164
12539
12422
1313
212698
18G73-107times77245
1840
2253
2437
2177
2580
4776
5915
4424
11673
10608
10459
10913
1358
3163
4286
2936
14253
15385
16374
15337
19G73-107times
CVJ-2-5-3-1-3
2324
2537
2691
2517
4958
5030
5599
5196
9524
9395
1114
610022
3241
3645
4444
3776
14483
14425
1674
515217
20G73-107times1305
2228
2433
2583
2415
6340
5794
6373
6169
12490
1015
8119
61
11536
4199
4199
5018
4472
18830
1595
218334
1770
6
21G73-107times(77371times
BSEC
TCP
-1)
1943
2128
2220
2097
3936
3980
5770
4562
11697
9858
10303
10619
1874
2369
4181
2808
15633
13839
16073
1518
222
G73-107times96AC
-93
2118
2218
2317
2217
4270
5843
6201
5438
10319
9872
1015
910117
2703
4460
4379
3847
14589
15715
16359
15555
23G73-107timesTo
go-II
2237
2365
2733
2445
5736
5175
6822
5911
12098
10808
1299
611967
3984
3670
5372
4342
17835
1598
219818
17878
24G73-107times99HS-18
2025
2081
2322
2143
3558
4229
6612
4800
12350
9685
11516
1118
42196
2782
5009
3329
1590
813914
1812
915983
2577245timesCV
J-2-5-3-1-3
2109
2360
2675
2381
6760
6558
7299
6872
15225
11861
1416
21374
94225
4752
5839
4939
2198
518418
21461
20621
2677245times1305
1919
2100
2197
2072
5700
5142
5347
5397
11687
10565
10737
10996
4318
3956
4244
4173
17386
1570
816085
16393
2777245times(77371times
BSEC
TCP
-1)
1851
1935
2020
1936
2577
2460
2838
2625
6989
4778
4636
5468
1631
1662
1999
1764
9566
7238
7474
8093
2877245times96AC
-93
1729
1780
2133
1881
5541
5058
5748
5449
11952
8174
9526
9884
3646
3587
4071
3768
17493
13232
15274
15333
2977245timesTo
go-II
2300
2508
2658
2489
3067
5431
5963
4820
11737
11021
1216
411641
1598
4053
4770
3474
14805
16452
1812
616461
3077245times99HS-18
1982
2019
2223
2075
3846
3119
4225
3730
7493
6720
6476
6896
3205
2578
2643
2809
11339
9839
1070
110626
12 Advances in Agriculture
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
31CV
J-2-5-3-1-3times1305
2406
2553
3067
2675
4178
4796
6281
5085
13822
11425
12252
12500
2472
3241
4907
3540
18000
16222
18532
17585
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
2142
2173
2238
2184
4522
5235
5640
5132
11673
9658
10248
10526
2614
3766
4178
3519
1619
514893
15888
15659
33CV
J-2-5-3-1-3times
96AC
-93
2350
2477
2600
2476
4676
4280
6090
5015
11975
9832
10868
10891
3097
2931
4445
3491
16651
14111
1695
815907
34CV
J-2-5-3-1-3times
Togo-II
2093
2666
2989
2583
4058
3727
5190
4325
12588
10717
11217
11507
2742
2570
4185
3166
16645
14443
16407
15832
35CV
J-2-5-3-1-3times
99HS-18
2028
2311
2386
2242
3287
2513
5356
3719
10055
9733
1036
410051
2004
1581
4251
2612
13342
12246
1572
01376
9
361305times(77371times
BSEC
TCP
-1)
2005
2173
2210
2129
3569
4692
5140
4467
11652
9826
10041
10507
2005
3665
4179
3283
15221
14518
1518
214974
371305times96AC
-93
1822
2003
2047
1957
5115
4023
4813
4650
9965
7675
7571
8404
3456
2647
3155
3086
15080
11698
12384
13054
381305timesTo
go-II
2545
2583
2644
2591
6885
7283
6795
6987
12265
11410
12583
12086
4500
6120
5350
5323
1915
018693
19378
19074
391305times99HS-18
2070
2408
2593
2357
5219
4332
4800
4784
1092
97677
8968
9192
3924
3332
3840
3699
1614
912009
1376
913976
40(77371times
BSEC
TCP
-1)
times96AC
-93
2056
2115
2157
2109
3158
4463
4717
4113
9166
7540
8100
8268
1880
3542
3347
2923
12324
12003
12817
12381
41(77371times
BSEC
TCP
-1)
timesTo
go-II
1868
2253
2450
2190
4422
6458
7038
5973
1415
312369
1379
713439
2291
4646
5586
4174
18575
18827
20835
19412
42(77371times
BSEC
TCP
-1)
times99HS-18
1995
2026
2209
2077
2720
2909
3543
3057
7392
4692
5023
5702
1766
2187
2147
2033
10112
7601
8566
8760
4396AC
-93timesTo
go-II
1953
2027
2511
2164
5972
4391
7126
5830
15537
1392
714432
14632
3780
2538
5655
3991
21510
18318
21558
20462
4496AC
-93times99HS-18
1952
2021
2242
2072
3465
2758
4496
3573
5959
5186
6094
5746
2707
2154
2409
2423
9424
7944
10590
9319
45To
go-IItimes
99HS-18
1932
2008
2658
2199
5381
4995
5436
5270
10299
8876
9244
9473
3986
3842
3668
3832
15680
13871
14680
1474
3Mean
1982
2146
2301
2143
3963
4123
4836
4307
9498
8087
8663
8749
2510
2914
3513
2979
13460
12210
13499
13056
SE243
198
245
mdash671
501
626
mdash959
750
822
mdash423
349
344
mdash1155
927
1167
mdashCD
087
071
087
mdash239
178
223
mdash342
267
293
mdash15
112
412
2mdash
411
330
416
mdash
Advances in Agriculture 13Ta
ble4Analysis
ofvaria
ncefor
10times10
half-diallelfor
different
charactersin
threee
nviro
nments
(a)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Germination
()
Rateof
emergence
STI()
SSTI
()
Num
bero
fleavesseedling
Seedlin
gheight
(cm)
Freshweight
(g)seedlin
gDry
weight
(g)seedlin
g
E 1
Replicates
2652731
596768
783393
20700
93018674
33764
1033999
002590
Treatm
ents
541097
0255lowastlowast
8181153lowastlowast
30863092lowastlowast
20680900lowastlowast
1100708lowastlowast
2106317lowastlowast
930829527lowastlowast
319767lowastlowast
Parents
95118
163lowastlowast
14289925lowastlowast
59114816lowastlowast
18880254lowastlowast
1084227lowastlowast
1087906lowastlowast
22561719lowastlowast
042162lowastlowast
Hybrid
s44
11423573lowastlowast
3398933lowastlowast
25697199lowastlowast
20633716lowastlowast
472874lowastlowast
1391526lowastlowast
674123518lowastlowast
261839lowastlowast
Parentsv
ersush
ybrid
s1
43693104lowastlowast
163619
916lowastlowast
3896838
3896
2793lowastlowast
28873741lowastlowast
4272
2798lowastlowast
234144289lowastlowast
5367022lowastlowast
Error
108
281184
1356832
1173684
674337
040104
306
462
2015
1619
005535
E 2
Replicates
2583819
3707389lowast
1596635
198738
034925
448213
899529
007177
Treatm
ents
549997443lowastlowast
2825177lowastlowast
35378177lowastlowast
19074306lowastlowast
665284lowastlowast
1764
081lowastlowast
257694570lowastlowast
2177519lowastlowast
Parents
96507784lowastlowast
3718
879lowastlowast
69866289lowastlowast
1798
5404lowastlowast
705379lowastlowast
1408518lowastlowast
10612519lowastlowast
077429lowastlowast
Hybrid
s44
10569731lowastlowast
2127302lowastlowast
2910
2537lowastlowast
19429240lowastlowast
329256lowastlowast
7000
03lowastlowast
186026552lowastlowast
1483428lowastlowast
Parentsv
ersush
ybrid
s1
16223710lowastlowast
25488361lowastlowast
1113
326
13257362lowastlowast
15089673lowastlowast
5178
3569lowastlowast
5634825791lowastlowast
51618348lowastlowast
Error
108
334760
1097039
1049505
635570
06764
116
0576
694613
006206
E 3
Replicates
2894398
4808815lowast
mdashmdash
051076
095989
3768421
030220
Treatm
ents
541261560
0lowastlowast
4842216lowastlowast
mdashmdash
2811077lowastlowast
5947316lowastlowast
3870844
50lowastlowast
3445076lowastlowast
Parents
97135
735lowastlowast
1097
6388lowastlowast
mdashmdash
1468699lowastlowast
1112
764lowastlowast
136081650lowastlowast
1307836lowastlowast
Hybrid
s44
13217740lowastlowast
1379360
mdashmdash
15540
71lowastlowast
4581632lowastlowast
252084767lowastlowast
2378032lowastlowast
Parentsv
ersush
ybrid
s1
35440
180lowastlowast
102000335lowastlowast
mdashmdash
70200767lowastlowast
109548362lowastlowast
8586095705lowastlowast
69630188lowastlowast
Error
108
782907
1085444
mdashmdash
139771
291325
3019
169
02400
4(b)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Panicle
emergence
Effectiv
etillersplant
Planth
eight
Earlength
Earw
eightp
lant
Dry
fodd
eryieldplant
Grain
yieldplant
Totalbiological
yieldplant
E 1
Replicates
231860
61lowastlowast
416348lowastlowast
1672
9472lowast
1706
411077192
9634539
392777
115544
59Treatm
ents
544152
368lowastlowast
085657lowastlowast
326706089lowastlowast
1602572lowastlowast
6177
0406lowastlowast
304113
204lowastlowast
34285030lowastlowast
575617
582lowastlowast
Parents
94511481lowastlowast
018538lowastlowast
140807476lowastlowast
2240093lowastlowast
11653
959lowastlowast
27014155lowastlowast
3858650lowastlowast
61321919lowastlowast
Hybrid
s44
3201347lowastlowast
100797lowastlowast
110395657lowastlowast
1010
497lowastlowast
40296671lowastlowast
171271411lowastlowast
2413
6880lowastlowast
302999650lowastlowast
Parentsv
ersush
ybrid
s1
4276
5286lowastlowast
02360
9lowast11517452
616lowastlowast
2191
6167lowastlowast
1457662749lowastlowast
8643043524lowastlowast
7546
41053lowastlowast
17199467546lowastlowast
Error
108
468159
004907
3692099
224610
17114
303498126
682350
5075268
E 2
Replicates
2656364
002556
61575565lowastlowast
828842lowastlowast
589094
2268506
260877
1507116
Treatm
ents
542999618lowastlowast
069822lowastlowast
268998723lowastlowast
2436886lowastlowast
60592784lowastlowast
247259877lowastlowast
4192
0073lowastlowast
513483489lowastlowast
Parents
926300
00lowastlowast
048079lowastlowast
165555
405lowastlowast
3871874lowastlowast
1412
2393lowastlowast
19543682lowastlowast
3132
426lowastlowast
57230706lowastlowast
Hybrid
s44
2533
165lowastlowast
07344
9lowastlowast
9616
2857lowastlowast
1463359lowastlowast
35495366lowastlowast
154759288lowastlowast
26262465lowastlowast
293492304lowastlowast
Parentsv
ersush
ybrid
s1
26850135lowastlowast
105929lowastlowast
880476
6697lowastlowast
32357161lowastlowast
1583112746lowastlowast
6366731560lowastlowast
107994
3646lowastlowast
14299370699lowastlowast
Error
108
507598
005829
5234122
149406
955133
2142411
462311
3273886
E 3
Replicates
2029697
021161
9459847
143746
985141
46800
6492
6334
3345160
Treatm
ents
548045499lowastlowast
094511lowastlowast
192420108lowastlowast
3279225lowastlowast
72049211lowastlowast
298953
021lowastlowast
55061308lowastlowast
651732994lowastlowast
Parents
98010
741lowastlowast
094166lowastlowast
116037343lowastlowast
3702185lowastlowast
16305190lowastlowast
2323644
0lowastlowast
4553
893lowastlowast
71484982lowastlowast
Hybrid
s44
6254377lowastlowast
095514lowastlowast
7378
1513lowastlowast
2188348lowastlowast
31813193lowastlowast
190263916lowastlowast
31572179lowastlowast
368689934lowastlowast
Parentsv
ersush
ybrid
s1
8716
7710lowastlowast
053496lowast
609996
3199lowastlowast
47471212lowastlowast
2344
130203lowastlowast
756272
2861lowastlowast
154314
9707lowastlowast
18327859
719lowastlowast
Error
108
696981
007883
5578505
228608
1491014
2574
223
449588
5187705
lowast
Sign
ificant
at119875=005
lowastlowastSign
ificant
at119875=001
14 Advances in Agriculture
Table 5 Mean performance of the genotypes for membrane thermostability in two environments
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594
Advances in Agriculture 15
Table 5 Continued
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434
Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash
lowastValues are transformed values
0
6
12
18
24
30
36
42
48
54
minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity
G73-10796AC-93CVJ-2-5-3-1-3
Togo-II99HS-18
H77833-2H7729-2772451305
H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305
1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305
H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305
77245 times 1305
77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305
77371 times BSECT CP-1
H77833-2 times G73-107
Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits
moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling
survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The
16 Advances in Agriculture
Table6Po
oled
correla
tioncoeffi
cientamon
gvario
uscharactersin
threee
nviro
nments
Characters
Germination
Rateof
emergence
STI
SSTI
Leaves
seedlin
gSeedlin
gheight
Fresh
weight
Dry
weight
MTS
Panicle
emergence
Effectiv
etillersplant
Plant
height
Earlength
Ear
weightp
lant
Dry
fodd
eryield
plant
Grain
yield
plant
Total
biological
yield
plant
Germination
1000
0357lowastlowast
0353lowastlowast
0590lowastlowast
0230
0373lowastlowast
0293lowast
0260
0276lowastminus0135minus0478lowastlowast
0326lowast
minus0069minus0104
minus0190
minus0145
minus0167
Rateof
emergence
1000
0233
0351lowastlowast
0605lowastlowast
0585lowastlowast
0551lowastlowast
0524lowastlowast
0139minus0593lowastlowast
0032
0517lowastlowast
0217
0388lowastlowast
0361lowastlowast
0348lowastlowast
0377lowastlowast
STI
1000
0837lowastlowast
0241
0129
0081
004
80890lowastlowast
minus0215
minus0211
0030minus0007minus0043
minus0101
minus0111
minus0084
SSTI
1000
0233
0264
0183
0140
0745lowastlowast
minus0247minus0268lowast
0184minus0017minus006
6minus0129
minus0144
minus0112
Num
bero
fleaves
1000
0683lowastlowast
0633lowastlowast
060
4lowastlowast
0176minus0474lowastlowast
0109
0576lowastlowast
0496lowastlowast
064
1lowastlowast
0607lowastlowast
0630lowastlowast
0630lowastlowast
Seedlin
gheight
1000
0703lowastlowast
0663lowastlowast
0031minus0493lowastlowast
minus0115
0735lowastlowast
0360lowastlowast
0526lowastlowast
0520lowastlowast
0515lowastlowast
0532lowastlowast
Freshweight
1000
0957lowastlowast
minus0016
0425lowastlowast
minus0082
0661lowastlowast
0351lowastlowast
0581lowastlowast
0496lowastlowast
0576lowastlowast
0533lowastlowast
Dry
weight
1000minus0033minus0428lowastlowast
minus0016
0634lowastlowast
0354lowastlowast
0620lowastlowast
0509lowastlowast
0614lowastlowast
0554lowastlowast
MTS
1000minus040
minus0177minus0037
0015
minus0074
minus0138
minus0131
minus0120
Panicle
emergence
1000
minus0150minus0269lowast
0022minus0329lowast
minus0337lowast
minus0280lowast
minus0341lowast
Effectiv
etillersplant
1000
minus0012
0170
0366lowastlowast
040
5lowastlowast
0323lowast
0401lowastlowast
Planth
eight
1000
0650lowastlowast
0603lowastlowast
0596lowastlowast
0619lowastlowast
0610lowastlowast
Earlength
1000
0608lowastlowast
0671lowastlowast
064
8lowastlowast
066
4lowastlowast
Earw
eightp
lant
1000
0910lowastlowast
0984lowastlowast
0957lowastlowast
Dry
fodd
eryield
plant
1000
0907lowastlowast
0991lowastlowast
Grain
yield
plant
1000
0950lowastlowast
Totalbiological
yield
plant
1000
lowastSign
ificant
at119875=005lowastlowastSign
ificant
at119875=001
Advances in Agriculture 17
faster germination and early vigour in seedling could act asphysical indicators for heat tolerance
Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits
Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics
Conflict of Interests
The authors declare that there is no conflict of interestsregarding the publication of this paper
References
[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981
[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993
[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006
[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993
[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013
[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007
[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965
[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011
[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001
[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009
[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962
[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967
[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963
[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001
Submit your manuscripts athttpwwwhindawicom
Nutrition and Metabolism
Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Food ScienceInternational Journal of
Agronomy
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
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International Journal of
Microbiology
The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom
Applied ampEnvironmentalSoil Science
Volume 2014
AgricultureAdvances in
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
BiodiversityInternational Journal of
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ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
GenomicsInternational Journal of
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Plant GenomicsInternational Journal of
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Biotechnology Research International
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Forestry ResearchInternational Journal of
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Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
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Advances in Agriculture 11
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
11H7729-2times77245
1847
2048
2200
2032
2467
3759
5466
3898
7672
7449
8921
8014
1356
2350
3796
2500
1013
911208
14387
11911
12H7729-2times
CVJ-2-5-3-1-3
2218
2585
2767
2523
3674
4372
4483
4176
10053
9467
8664
9395
2417
3312
3345
3025
1372
813839
1314
713571
13H7729-2times1305
2169
2338
2547
2352
6728
6166
6792
6562
13333
11662
13301
1276
54128
4501
5660
4763
20061
17828
20093
19327
14H7729-2times(77371times
BSEC
TCP
-1)
1925
2095
2127
2049
3530
4165
4649
4115
9638
6917
7698
8084
1681
2833
3150
2555
1316
8110
82
12347
1219
915
H7729-2times96AC
-93
2012
2070
2180
2087
4955
4447
4596
4666
8738
6882
7762
7794
3754
3558
3181
3498
13693
11329
12359
12460
16H7729-2timesTo
go-II
2138
2428
2563
2377
4949
4660
5118
4909
8549
7243
7191
7661
3586
3353
3028
3322
13498
11903
12310
12570
17H7729-2times99HS-18
1899
1933
2073
1969
4046
4527
4988
4520
8492
7895
8144
8177
2561
3378
3553
3164
12539
12422
1313
212698
18G73-107times77245
1840
2253
2437
2177
2580
4776
5915
4424
11673
10608
10459
10913
1358
3163
4286
2936
14253
15385
16374
15337
19G73-107times
CVJ-2-5-3-1-3
2324
2537
2691
2517
4958
5030
5599
5196
9524
9395
1114
610022
3241
3645
4444
3776
14483
14425
1674
515217
20G73-107times1305
2228
2433
2583
2415
6340
5794
6373
6169
12490
1015
8119
61
11536
4199
4199
5018
4472
18830
1595
218334
1770
6
21G73-107times(77371times
BSEC
TCP
-1)
1943
2128
2220
2097
3936
3980
5770
4562
11697
9858
10303
10619
1874
2369
4181
2808
15633
13839
16073
1518
222
G73-107times96AC
-93
2118
2218
2317
2217
4270
5843
6201
5438
10319
9872
1015
910117
2703
4460
4379
3847
14589
15715
16359
15555
23G73-107timesTo
go-II
2237
2365
2733
2445
5736
5175
6822
5911
12098
10808
1299
611967
3984
3670
5372
4342
17835
1598
219818
17878
24G73-107times99HS-18
2025
2081
2322
2143
3558
4229
6612
4800
12350
9685
11516
1118
42196
2782
5009
3329
1590
813914
1812
915983
2577245timesCV
J-2-5-3-1-3
2109
2360
2675
2381
6760
6558
7299
6872
15225
11861
1416
21374
94225
4752
5839
4939
2198
518418
21461
20621
2677245times1305
1919
2100
2197
2072
5700
5142
5347
5397
11687
10565
10737
10996
4318
3956
4244
4173
17386
1570
816085
16393
2777245times(77371times
BSEC
TCP
-1)
1851
1935
2020
1936
2577
2460
2838
2625
6989
4778
4636
5468
1631
1662
1999
1764
9566
7238
7474
8093
2877245times96AC
-93
1729
1780
2133
1881
5541
5058
5748
5449
11952
8174
9526
9884
3646
3587
4071
3768
17493
13232
15274
15333
2977245timesTo
go-II
2300
2508
2658
2489
3067
5431
5963
4820
11737
11021
1216
411641
1598
4053
4770
3474
14805
16452
1812
616461
3077245times99HS-18
1982
2019
2223
2075
3846
3119
4225
3730
7493
6720
6476
6896
3205
2578
2643
2809
11339
9839
1070
110626
12 Advances in Agriculture
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
31CV
J-2-5-3-1-3times1305
2406
2553
3067
2675
4178
4796
6281
5085
13822
11425
12252
12500
2472
3241
4907
3540
18000
16222
18532
17585
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
2142
2173
2238
2184
4522
5235
5640
5132
11673
9658
10248
10526
2614
3766
4178
3519
1619
514893
15888
15659
33CV
J-2-5-3-1-3times
96AC
-93
2350
2477
2600
2476
4676
4280
6090
5015
11975
9832
10868
10891
3097
2931
4445
3491
16651
14111
1695
815907
34CV
J-2-5-3-1-3times
Togo-II
2093
2666
2989
2583
4058
3727
5190
4325
12588
10717
11217
11507
2742
2570
4185
3166
16645
14443
16407
15832
35CV
J-2-5-3-1-3times
99HS-18
2028
2311
2386
2242
3287
2513
5356
3719
10055
9733
1036
410051
2004
1581
4251
2612
13342
12246
1572
01376
9
361305times(77371times
BSEC
TCP
-1)
2005
2173
2210
2129
3569
4692
5140
4467
11652
9826
10041
10507
2005
3665
4179
3283
15221
14518
1518
214974
371305times96AC
-93
1822
2003
2047
1957
5115
4023
4813
4650
9965
7675
7571
8404
3456
2647
3155
3086
15080
11698
12384
13054
381305timesTo
go-II
2545
2583
2644
2591
6885
7283
6795
6987
12265
11410
12583
12086
4500
6120
5350
5323
1915
018693
19378
19074
391305times99HS-18
2070
2408
2593
2357
5219
4332
4800
4784
1092
97677
8968
9192
3924
3332
3840
3699
1614
912009
1376
913976
40(77371times
BSEC
TCP
-1)
times96AC
-93
2056
2115
2157
2109
3158
4463
4717
4113
9166
7540
8100
8268
1880
3542
3347
2923
12324
12003
12817
12381
41(77371times
BSEC
TCP
-1)
timesTo
go-II
1868
2253
2450
2190
4422
6458
7038
5973
1415
312369
1379
713439
2291
4646
5586
4174
18575
18827
20835
19412
42(77371times
BSEC
TCP
-1)
times99HS-18
1995
2026
2209
2077
2720
2909
3543
3057
7392
4692
5023
5702
1766
2187
2147
2033
10112
7601
8566
8760
4396AC
-93timesTo
go-II
1953
2027
2511
2164
5972
4391
7126
5830
15537
1392
714432
14632
3780
2538
5655
3991
21510
18318
21558
20462
4496AC
-93times99HS-18
1952
2021
2242
2072
3465
2758
4496
3573
5959
5186
6094
5746
2707
2154
2409
2423
9424
7944
10590
9319
45To
go-IItimes
99HS-18
1932
2008
2658
2199
5381
4995
5436
5270
10299
8876
9244
9473
3986
3842
3668
3832
15680
13871
14680
1474
3Mean
1982
2146
2301
2143
3963
4123
4836
4307
9498
8087
8663
8749
2510
2914
3513
2979
13460
12210
13499
13056
SE243
198
245
mdash671
501
626
mdash959
750
822
mdash423
349
344
mdash1155
927
1167
mdashCD
087
071
087
mdash239
178
223
mdash342
267
293
mdash15
112
412
2mdash
411
330
416
mdash
Advances in Agriculture 13Ta
ble4Analysis
ofvaria
ncefor
10times10
half-diallelfor
different
charactersin
threee
nviro
nments
(a)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Germination
()
Rateof
emergence
STI()
SSTI
()
Num
bero
fleavesseedling
Seedlin
gheight
(cm)
Freshweight
(g)seedlin
gDry
weight
(g)seedlin
g
E 1
Replicates
2652731
596768
783393
20700
93018674
33764
1033999
002590
Treatm
ents
541097
0255lowastlowast
8181153lowastlowast
30863092lowastlowast
20680900lowastlowast
1100708lowastlowast
2106317lowastlowast
930829527lowastlowast
319767lowastlowast
Parents
95118
163lowastlowast
14289925lowastlowast
59114816lowastlowast
18880254lowastlowast
1084227lowastlowast
1087906lowastlowast
22561719lowastlowast
042162lowastlowast
Hybrid
s44
11423573lowastlowast
3398933lowastlowast
25697199lowastlowast
20633716lowastlowast
472874lowastlowast
1391526lowastlowast
674123518lowastlowast
261839lowastlowast
Parentsv
ersush
ybrid
s1
43693104lowastlowast
163619
916lowastlowast
3896838
3896
2793lowastlowast
28873741lowastlowast
4272
2798lowastlowast
234144289lowastlowast
5367022lowastlowast
Error
108
281184
1356832
1173684
674337
040104
306
462
2015
1619
005535
E 2
Replicates
2583819
3707389lowast
1596635
198738
034925
448213
899529
007177
Treatm
ents
549997443lowastlowast
2825177lowastlowast
35378177lowastlowast
19074306lowastlowast
665284lowastlowast
1764
081lowastlowast
257694570lowastlowast
2177519lowastlowast
Parents
96507784lowastlowast
3718
879lowastlowast
69866289lowastlowast
1798
5404lowastlowast
705379lowastlowast
1408518lowastlowast
10612519lowastlowast
077429lowastlowast
Hybrid
s44
10569731lowastlowast
2127302lowastlowast
2910
2537lowastlowast
19429240lowastlowast
329256lowastlowast
7000
03lowastlowast
186026552lowastlowast
1483428lowastlowast
Parentsv
ersush
ybrid
s1
16223710lowastlowast
25488361lowastlowast
1113
326
13257362lowastlowast
15089673lowastlowast
5178
3569lowastlowast
5634825791lowastlowast
51618348lowastlowast
Error
108
334760
1097039
1049505
635570
06764
116
0576
694613
006206
E 3
Replicates
2894398
4808815lowast
mdashmdash
051076
095989
3768421
030220
Treatm
ents
541261560
0lowastlowast
4842216lowastlowast
mdashmdash
2811077lowastlowast
5947316lowastlowast
3870844
50lowastlowast
3445076lowastlowast
Parents
97135
735lowastlowast
1097
6388lowastlowast
mdashmdash
1468699lowastlowast
1112
764lowastlowast
136081650lowastlowast
1307836lowastlowast
Hybrid
s44
13217740lowastlowast
1379360
mdashmdash
15540
71lowastlowast
4581632lowastlowast
252084767lowastlowast
2378032lowastlowast
Parentsv
ersush
ybrid
s1
35440
180lowastlowast
102000335lowastlowast
mdashmdash
70200767lowastlowast
109548362lowastlowast
8586095705lowastlowast
69630188lowastlowast
Error
108
782907
1085444
mdashmdash
139771
291325
3019
169
02400
4(b)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Panicle
emergence
Effectiv
etillersplant
Planth
eight
Earlength
Earw
eightp
lant
Dry
fodd
eryieldplant
Grain
yieldplant
Totalbiological
yieldplant
E 1
Replicates
231860
61lowastlowast
416348lowastlowast
1672
9472lowast
1706
411077192
9634539
392777
115544
59Treatm
ents
544152
368lowastlowast
085657lowastlowast
326706089lowastlowast
1602572lowastlowast
6177
0406lowastlowast
304113
204lowastlowast
34285030lowastlowast
575617
582lowastlowast
Parents
94511481lowastlowast
018538lowastlowast
140807476lowastlowast
2240093lowastlowast
11653
959lowastlowast
27014155lowastlowast
3858650lowastlowast
61321919lowastlowast
Hybrid
s44
3201347lowastlowast
100797lowastlowast
110395657lowastlowast
1010
497lowastlowast
40296671lowastlowast
171271411lowastlowast
2413
6880lowastlowast
302999650lowastlowast
Parentsv
ersush
ybrid
s1
4276
5286lowastlowast
02360
9lowast11517452
616lowastlowast
2191
6167lowastlowast
1457662749lowastlowast
8643043524lowastlowast
7546
41053lowastlowast
17199467546lowastlowast
Error
108
468159
004907
3692099
224610
17114
303498126
682350
5075268
E 2
Replicates
2656364
002556
61575565lowastlowast
828842lowastlowast
589094
2268506
260877
1507116
Treatm
ents
542999618lowastlowast
069822lowastlowast
268998723lowastlowast
2436886lowastlowast
60592784lowastlowast
247259877lowastlowast
4192
0073lowastlowast
513483489lowastlowast
Parents
926300
00lowastlowast
048079lowastlowast
165555
405lowastlowast
3871874lowastlowast
1412
2393lowastlowast
19543682lowastlowast
3132
426lowastlowast
57230706lowastlowast
Hybrid
s44
2533
165lowastlowast
07344
9lowastlowast
9616
2857lowastlowast
1463359lowastlowast
35495366lowastlowast
154759288lowastlowast
26262465lowastlowast
293492304lowastlowast
Parentsv
ersush
ybrid
s1
26850135lowastlowast
105929lowastlowast
880476
6697lowastlowast
32357161lowastlowast
1583112746lowastlowast
6366731560lowastlowast
107994
3646lowastlowast
14299370699lowastlowast
Error
108
507598
005829
5234122
149406
955133
2142411
462311
3273886
E 3
Replicates
2029697
021161
9459847
143746
985141
46800
6492
6334
3345160
Treatm
ents
548045499lowastlowast
094511lowastlowast
192420108lowastlowast
3279225lowastlowast
72049211lowastlowast
298953
021lowastlowast
55061308lowastlowast
651732994lowastlowast
Parents
98010
741lowastlowast
094166lowastlowast
116037343lowastlowast
3702185lowastlowast
16305190lowastlowast
2323644
0lowastlowast
4553
893lowastlowast
71484982lowastlowast
Hybrid
s44
6254377lowastlowast
095514lowastlowast
7378
1513lowastlowast
2188348lowastlowast
31813193lowastlowast
190263916lowastlowast
31572179lowastlowast
368689934lowastlowast
Parentsv
ersush
ybrid
s1
8716
7710lowastlowast
053496lowast
609996
3199lowastlowast
47471212lowastlowast
2344
130203lowastlowast
756272
2861lowastlowast
154314
9707lowastlowast
18327859
719lowastlowast
Error
108
696981
007883
5578505
228608
1491014
2574
223
449588
5187705
lowast
Sign
ificant
at119875=005
lowastlowastSign
ificant
at119875=001
14 Advances in Agriculture
Table 5 Mean performance of the genotypes for membrane thermostability in two environments
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594
Advances in Agriculture 15
Table 5 Continued
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434
Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash
lowastValues are transformed values
0
6
12
18
24
30
36
42
48
54
minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity
G73-10796AC-93CVJ-2-5-3-1-3
Togo-II99HS-18
H77833-2H7729-2772451305
H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305
1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305
H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305
77245 times 1305
77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305
77371 times BSECT CP-1
H77833-2 times G73-107
Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits
moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling
survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The
16 Advances in Agriculture
Table6Po
oled
correla
tioncoeffi
cientamon
gvario
uscharactersin
threee
nviro
nments
Characters
Germination
Rateof
emergence
STI
SSTI
Leaves
seedlin
gSeedlin
gheight
Fresh
weight
Dry
weight
MTS
Panicle
emergence
Effectiv
etillersplant
Plant
height
Earlength
Ear
weightp
lant
Dry
fodd
eryield
plant
Grain
yield
plant
Total
biological
yield
plant
Germination
1000
0357lowastlowast
0353lowastlowast
0590lowastlowast
0230
0373lowastlowast
0293lowast
0260
0276lowastminus0135minus0478lowastlowast
0326lowast
minus0069minus0104
minus0190
minus0145
minus0167
Rateof
emergence
1000
0233
0351lowastlowast
0605lowastlowast
0585lowastlowast
0551lowastlowast
0524lowastlowast
0139minus0593lowastlowast
0032
0517lowastlowast
0217
0388lowastlowast
0361lowastlowast
0348lowastlowast
0377lowastlowast
STI
1000
0837lowastlowast
0241
0129
0081
004
80890lowastlowast
minus0215
minus0211
0030minus0007minus0043
minus0101
minus0111
minus0084
SSTI
1000
0233
0264
0183
0140
0745lowastlowast
minus0247minus0268lowast
0184minus0017minus006
6minus0129
minus0144
minus0112
Num
bero
fleaves
1000
0683lowastlowast
0633lowastlowast
060
4lowastlowast
0176minus0474lowastlowast
0109
0576lowastlowast
0496lowastlowast
064
1lowastlowast
0607lowastlowast
0630lowastlowast
0630lowastlowast
Seedlin
gheight
1000
0703lowastlowast
0663lowastlowast
0031minus0493lowastlowast
minus0115
0735lowastlowast
0360lowastlowast
0526lowastlowast
0520lowastlowast
0515lowastlowast
0532lowastlowast
Freshweight
1000
0957lowastlowast
minus0016
0425lowastlowast
minus0082
0661lowastlowast
0351lowastlowast
0581lowastlowast
0496lowastlowast
0576lowastlowast
0533lowastlowast
Dry
weight
1000minus0033minus0428lowastlowast
minus0016
0634lowastlowast
0354lowastlowast
0620lowastlowast
0509lowastlowast
0614lowastlowast
0554lowastlowast
MTS
1000minus040
minus0177minus0037
0015
minus0074
minus0138
minus0131
minus0120
Panicle
emergence
1000
minus0150minus0269lowast
0022minus0329lowast
minus0337lowast
minus0280lowast
minus0341lowast
Effectiv
etillersplant
1000
minus0012
0170
0366lowastlowast
040
5lowastlowast
0323lowast
0401lowastlowast
Planth
eight
1000
0650lowastlowast
0603lowastlowast
0596lowastlowast
0619lowastlowast
0610lowastlowast
Earlength
1000
0608lowastlowast
0671lowastlowast
064
8lowastlowast
066
4lowastlowast
Earw
eightp
lant
1000
0910lowastlowast
0984lowastlowast
0957lowastlowast
Dry
fodd
eryield
plant
1000
0907lowastlowast
0991lowastlowast
Grain
yield
plant
1000
0950lowastlowast
Totalbiological
yield
plant
1000
lowastSign
ificant
at119875=005lowastlowastSign
ificant
at119875=001
Advances in Agriculture 17
faster germination and early vigour in seedling could act asphysical indicators for heat tolerance
Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits
Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics
Conflict of Interests
The authors declare that there is no conflict of interestsregarding the publication of this paper
References
[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981
[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993
[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006
[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993
[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013
[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007
[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965
[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011
[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001
[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009
[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962
[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967
[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963
[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001
Submit your manuscripts athttpwwwhindawicom
Nutrition and Metabolism
Journal of
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Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
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Agronomy
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
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International Journal of
Microbiology
The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom
Applied ampEnvironmentalSoil Science
Volume 2014
AgricultureAdvances in
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
BiodiversityInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
GenomicsInternational Journal of
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Plant GenomicsInternational Journal of
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Biotechnology Research International
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Forestry ResearchInternational Journal of
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Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
EcologyInternational Journal of
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Evolutionary BiologyInternational Journal of
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12 Advances in Agriculture
(c)Con
tinued
Sr
No
Genotypes
Earheadleng
th(cm)
Earheadweight(g)plant
Dry
fodd
eryield(g)plant
Grain
yield(g)plant
Totalbiologicalyield
(g)plant
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
E 1E 2
E 3Mean
31CV
J-2-5-3-1-3times1305
2406
2553
3067
2675
4178
4796
6281
5085
13822
11425
12252
12500
2472
3241
4907
3540
18000
16222
18532
17585
32CV
J-2-5-3-1-3times
(77371times
BSEC
TCP
-1)
2142
2173
2238
2184
4522
5235
5640
5132
11673
9658
10248
10526
2614
3766
4178
3519
1619
514893
15888
15659
33CV
J-2-5-3-1-3times
96AC
-93
2350
2477
2600
2476
4676
4280
6090
5015
11975
9832
10868
10891
3097
2931
4445
3491
16651
14111
1695
815907
34CV
J-2-5-3-1-3times
Togo-II
2093
2666
2989
2583
4058
3727
5190
4325
12588
10717
11217
11507
2742
2570
4185
3166
16645
14443
16407
15832
35CV
J-2-5-3-1-3times
99HS-18
2028
2311
2386
2242
3287
2513
5356
3719
10055
9733
1036
410051
2004
1581
4251
2612
13342
12246
1572
01376
9
361305times(77371times
BSEC
TCP
-1)
2005
2173
2210
2129
3569
4692
5140
4467
11652
9826
10041
10507
2005
3665
4179
3283
15221
14518
1518
214974
371305times96AC
-93
1822
2003
2047
1957
5115
4023
4813
4650
9965
7675
7571
8404
3456
2647
3155
3086
15080
11698
12384
13054
381305timesTo
go-II
2545
2583
2644
2591
6885
7283
6795
6987
12265
11410
12583
12086
4500
6120
5350
5323
1915
018693
19378
19074
391305times99HS-18
2070
2408
2593
2357
5219
4332
4800
4784
1092
97677
8968
9192
3924
3332
3840
3699
1614
912009
1376
913976
40(77371times
BSEC
TCP
-1)
times96AC
-93
2056
2115
2157
2109
3158
4463
4717
4113
9166
7540
8100
8268
1880
3542
3347
2923
12324
12003
12817
12381
41(77371times
BSEC
TCP
-1)
timesTo
go-II
1868
2253
2450
2190
4422
6458
7038
5973
1415
312369
1379
713439
2291
4646
5586
4174
18575
18827
20835
19412
42(77371times
BSEC
TCP
-1)
times99HS-18
1995
2026
2209
2077
2720
2909
3543
3057
7392
4692
5023
5702
1766
2187
2147
2033
10112
7601
8566
8760
4396AC
-93timesTo
go-II
1953
2027
2511
2164
5972
4391
7126
5830
15537
1392
714432
14632
3780
2538
5655
3991
21510
18318
21558
20462
4496AC
-93times99HS-18
1952
2021
2242
2072
3465
2758
4496
3573
5959
5186
6094
5746
2707
2154
2409
2423
9424
7944
10590
9319
45To
go-IItimes
99HS-18
1932
2008
2658
2199
5381
4995
5436
5270
10299
8876
9244
9473
3986
3842
3668
3832
15680
13871
14680
1474
3Mean
1982
2146
2301
2143
3963
4123
4836
4307
9498
8087
8663
8749
2510
2914
3513
2979
13460
12210
13499
13056
SE243
198
245
mdash671
501
626
mdash959
750
822
mdash423
349
344
mdash1155
927
1167
mdashCD
087
071
087
mdash239
178
223
mdash342
267
293
mdash15
112
412
2mdash
411
330
416
mdash
Advances in Agriculture 13Ta
ble4Analysis
ofvaria
ncefor
10times10
half-diallelfor
different
charactersin
threee
nviro
nments
(a)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Germination
()
Rateof
emergence
STI()
SSTI
()
Num
bero
fleavesseedling
Seedlin
gheight
(cm)
Freshweight
(g)seedlin
gDry
weight
(g)seedlin
g
E 1
Replicates
2652731
596768
783393
20700
93018674
33764
1033999
002590
Treatm
ents
541097
0255lowastlowast
8181153lowastlowast
30863092lowastlowast
20680900lowastlowast
1100708lowastlowast
2106317lowastlowast
930829527lowastlowast
319767lowastlowast
Parents
95118
163lowastlowast
14289925lowastlowast
59114816lowastlowast
18880254lowastlowast
1084227lowastlowast
1087906lowastlowast
22561719lowastlowast
042162lowastlowast
Hybrid
s44
11423573lowastlowast
3398933lowastlowast
25697199lowastlowast
20633716lowastlowast
472874lowastlowast
1391526lowastlowast
674123518lowastlowast
261839lowastlowast
Parentsv
ersush
ybrid
s1
43693104lowastlowast
163619
916lowastlowast
3896838
3896
2793lowastlowast
28873741lowastlowast
4272
2798lowastlowast
234144289lowastlowast
5367022lowastlowast
Error
108
281184
1356832
1173684
674337
040104
306
462
2015
1619
005535
E 2
Replicates
2583819
3707389lowast
1596635
198738
034925
448213
899529
007177
Treatm
ents
549997443lowastlowast
2825177lowastlowast
35378177lowastlowast
19074306lowastlowast
665284lowastlowast
1764
081lowastlowast
257694570lowastlowast
2177519lowastlowast
Parents
96507784lowastlowast
3718
879lowastlowast
69866289lowastlowast
1798
5404lowastlowast
705379lowastlowast
1408518lowastlowast
10612519lowastlowast
077429lowastlowast
Hybrid
s44
10569731lowastlowast
2127302lowastlowast
2910
2537lowastlowast
19429240lowastlowast
329256lowastlowast
7000
03lowastlowast
186026552lowastlowast
1483428lowastlowast
Parentsv
ersush
ybrid
s1
16223710lowastlowast
25488361lowastlowast
1113
326
13257362lowastlowast
15089673lowastlowast
5178
3569lowastlowast
5634825791lowastlowast
51618348lowastlowast
Error
108
334760
1097039
1049505
635570
06764
116
0576
694613
006206
E 3
Replicates
2894398
4808815lowast
mdashmdash
051076
095989
3768421
030220
Treatm
ents
541261560
0lowastlowast
4842216lowastlowast
mdashmdash
2811077lowastlowast
5947316lowastlowast
3870844
50lowastlowast
3445076lowastlowast
Parents
97135
735lowastlowast
1097
6388lowastlowast
mdashmdash
1468699lowastlowast
1112
764lowastlowast
136081650lowastlowast
1307836lowastlowast
Hybrid
s44
13217740lowastlowast
1379360
mdashmdash
15540
71lowastlowast
4581632lowastlowast
252084767lowastlowast
2378032lowastlowast
Parentsv
ersush
ybrid
s1
35440
180lowastlowast
102000335lowastlowast
mdashmdash
70200767lowastlowast
109548362lowastlowast
8586095705lowastlowast
69630188lowastlowast
Error
108
782907
1085444
mdashmdash
139771
291325
3019
169
02400
4(b)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Panicle
emergence
Effectiv
etillersplant
Planth
eight
Earlength
Earw
eightp
lant
Dry
fodd
eryieldplant
Grain
yieldplant
Totalbiological
yieldplant
E 1
Replicates
231860
61lowastlowast
416348lowastlowast
1672
9472lowast
1706
411077192
9634539
392777
115544
59Treatm
ents
544152
368lowastlowast
085657lowastlowast
326706089lowastlowast
1602572lowastlowast
6177
0406lowastlowast
304113
204lowastlowast
34285030lowastlowast
575617
582lowastlowast
Parents
94511481lowastlowast
018538lowastlowast
140807476lowastlowast
2240093lowastlowast
11653
959lowastlowast
27014155lowastlowast
3858650lowastlowast
61321919lowastlowast
Hybrid
s44
3201347lowastlowast
100797lowastlowast
110395657lowastlowast
1010
497lowastlowast
40296671lowastlowast
171271411lowastlowast
2413
6880lowastlowast
302999650lowastlowast
Parentsv
ersush
ybrid
s1
4276
5286lowastlowast
02360
9lowast11517452
616lowastlowast
2191
6167lowastlowast
1457662749lowastlowast
8643043524lowastlowast
7546
41053lowastlowast
17199467546lowastlowast
Error
108
468159
004907
3692099
224610
17114
303498126
682350
5075268
E 2
Replicates
2656364
002556
61575565lowastlowast
828842lowastlowast
589094
2268506
260877
1507116
Treatm
ents
542999618lowastlowast
069822lowastlowast
268998723lowastlowast
2436886lowastlowast
60592784lowastlowast
247259877lowastlowast
4192
0073lowastlowast
513483489lowastlowast
Parents
926300
00lowastlowast
048079lowastlowast
165555
405lowastlowast
3871874lowastlowast
1412
2393lowastlowast
19543682lowastlowast
3132
426lowastlowast
57230706lowastlowast
Hybrid
s44
2533
165lowastlowast
07344
9lowastlowast
9616
2857lowastlowast
1463359lowastlowast
35495366lowastlowast
154759288lowastlowast
26262465lowastlowast
293492304lowastlowast
Parentsv
ersush
ybrid
s1
26850135lowastlowast
105929lowastlowast
880476
6697lowastlowast
32357161lowastlowast
1583112746lowastlowast
6366731560lowastlowast
107994
3646lowastlowast
14299370699lowastlowast
Error
108
507598
005829
5234122
149406
955133
2142411
462311
3273886
E 3
Replicates
2029697
021161
9459847
143746
985141
46800
6492
6334
3345160
Treatm
ents
548045499lowastlowast
094511lowastlowast
192420108lowastlowast
3279225lowastlowast
72049211lowastlowast
298953
021lowastlowast
55061308lowastlowast
651732994lowastlowast
Parents
98010
741lowastlowast
094166lowastlowast
116037343lowastlowast
3702185lowastlowast
16305190lowastlowast
2323644
0lowastlowast
4553
893lowastlowast
71484982lowastlowast
Hybrid
s44
6254377lowastlowast
095514lowastlowast
7378
1513lowastlowast
2188348lowastlowast
31813193lowastlowast
190263916lowastlowast
31572179lowastlowast
368689934lowastlowast
Parentsv
ersush
ybrid
s1
8716
7710lowastlowast
053496lowast
609996
3199lowastlowast
47471212lowastlowast
2344
130203lowastlowast
756272
2861lowastlowast
154314
9707lowastlowast
18327859
719lowastlowast
Error
108
696981
007883
5578505
228608
1491014
2574
223
449588
5187705
lowast
Sign
ificant
at119875=005
lowastlowastSign
ificant
at119875=001
14 Advances in Agriculture
Table 5 Mean performance of the genotypes for membrane thermostability in two environments
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594
Advances in Agriculture 15
Table 5 Continued
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434
Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash
lowastValues are transformed values
0
6
12
18
24
30
36
42
48
54
minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity
G73-10796AC-93CVJ-2-5-3-1-3
Togo-II99HS-18
H77833-2H7729-2772451305
H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305
1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305
H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305
77245 times 1305
77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305
77371 times BSECT CP-1
H77833-2 times G73-107
Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits
moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling
survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The
16 Advances in Agriculture
Table6Po
oled
correla
tioncoeffi
cientamon
gvario
uscharactersin
threee
nviro
nments
Characters
Germination
Rateof
emergence
STI
SSTI
Leaves
seedlin
gSeedlin
gheight
Fresh
weight
Dry
weight
MTS
Panicle
emergence
Effectiv
etillersplant
Plant
height
Earlength
Ear
weightp
lant
Dry
fodd
eryield
plant
Grain
yield
plant
Total
biological
yield
plant
Germination
1000
0357lowastlowast
0353lowastlowast
0590lowastlowast
0230
0373lowastlowast
0293lowast
0260
0276lowastminus0135minus0478lowastlowast
0326lowast
minus0069minus0104
minus0190
minus0145
minus0167
Rateof
emergence
1000
0233
0351lowastlowast
0605lowastlowast
0585lowastlowast
0551lowastlowast
0524lowastlowast
0139minus0593lowastlowast
0032
0517lowastlowast
0217
0388lowastlowast
0361lowastlowast
0348lowastlowast
0377lowastlowast
STI
1000
0837lowastlowast
0241
0129
0081
004
80890lowastlowast
minus0215
minus0211
0030minus0007minus0043
minus0101
minus0111
minus0084
SSTI
1000
0233
0264
0183
0140
0745lowastlowast
minus0247minus0268lowast
0184minus0017minus006
6minus0129
minus0144
minus0112
Num
bero
fleaves
1000
0683lowastlowast
0633lowastlowast
060
4lowastlowast
0176minus0474lowastlowast
0109
0576lowastlowast
0496lowastlowast
064
1lowastlowast
0607lowastlowast
0630lowastlowast
0630lowastlowast
Seedlin
gheight
1000
0703lowastlowast
0663lowastlowast
0031minus0493lowastlowast
minus0115
0735lowastlowast
0360lowastlowast
0526lowastlowast
0520lowastlowast
0515lowastlowast
0532lowastlowast
Freshweight
1000
0957lowastlowast
minus0016
0425lowastlowast
minus0082
0661lowastlowast
0351lowastlowast
0581lowastlowast
0496lowastlowast
0576lowastlowast
0533lowastlowast
Dry
weight
1000minus0033minus0428lowastlowast
minus0016
0634lowastlowast
0354lowastlowast
0620lowastlowast
0509lowastlowast
0614lowastlowast
0554lowastlowast
MTS
1000minus040
minus0177minus0037
0015
minus0074
minus0138
minus0131
minus0120
Panicle
emergence
1000
minus0150minus0269lowast
0022minus0329lowast
minus0337lowast
minus0280lowast
minus0341lowast
Effectiv
etillersplant
1000
minus0012
0170
0366lowastlowast
040
5lowastlowast
0323lowast
0401lowastlowast
Planth
eight
1000
0650lowastlowast
0603lowastlowast
0596lowastlowast
0619lowastlowast
0610lowastlowast
Earlength
1000
0608lowastlowast
0671lowastlowast
064
8lowastlowast
066
4lowastlowast
Earw
eightp
lant
1000
0910lowastlowast
0984lowastlowast
0957lowastlowast
Dry
fodd
eryield
plant
1000
0907lowastlowast
0991lowastlowast
Grain
yield
plant
1000
0950lowastlowast
Totalbiological
yield
plant
1000
lowastSign
ificant
at119875=005lowastlowastSign
ificant
at119875=001
Advances in Agriculture 17
faster germination and early vigour in seedling could act asphysical indicators for heat tolerance
Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits
Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics
Conflict of Interests
The authors declare that there is no conflict of interestsregarding the publication of this paper
References
[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981
[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993
[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006
[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993
[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013
[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007
[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965
[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011
[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001
[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009
[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962
[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967
[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963
[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001
Submit your manuscripts athttpwwwhindawicom
Nutrition and Metabolism
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Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
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Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
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International Journal of
Microbiology
The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom
Applied ampEnvironmentalSoil Science
Volume 2014
AgricultureAdvances in
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PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
BiodiversityInternational Journal of
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ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
GenomicsInternational Journal of
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Plant GenomicsInternational Journal of
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Biotechnology Research International
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Advances in Agriculture 13Ta
ble4Analysis
ofvaria
ncefor
10times10
half-diallelfor
different
charactersin
threee
nviro
nments
(a)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Germination
()
Rateof
emergence
STI()
SSTI
()
Num
bero
fleavesseedling
Seedlin
gheight
(cm)
Freshweight
(g)seedlin
gDry
weight
(g)seedlin
g
E 1
Replicates
2652731
596768
783393
20700
93018674
33764
1033999
002590
Treatm
ents
541097
0255lowastlowast
8181153lowastlowast
30863092lowastlowast
20680900lowastlowast
1100708lowastlowast
2106317lowastlowast
930829527lowastlowast
319767lowastlowast
Parents
95118
163lowastlowast
14289925lowastlowast
59114816lowastlowast
18880254lowastlowast
1084227lowastlowast
1087906lowastlowast
22561719lowastlowast
042162lowastlowast
Hybrid
s44
11423573lowastlowast
3398933lowastlowast
25697199lowastlowast
20633716lowastlowast
472874lowastlowast
1391526lowastlowast
674123518lowastlowast
261839lowastlowast
Parentsv
ersush
ybrid
s1
43693104lowastlowast
163619
916lowastlowast
3896838
3896
2793lowastlowast
28873741lowastlowast
4272
2798lowastlowast
234144289lowastlowast
5367022lowastlowast
Error
108
281184
1356832
1173684
674337
040104
306
462
2015
1619
005535
E 2
Replicates
2583819
3707389lowast
1596635
198738
034925
448213
899529
007177
Treatm
ents
549997443lowastlowast
2825177lowastlowast
35378177lowastlowast
19074306lowastlowast
665284lowastlowast
1764
081lowastlowast
257694570lowastlowast
2177519lowastlowast
Parents
96507784lowastlowast
3718
879lowastlowast
69866289lowastlowast
1798
5404lowastlowast
705379lowastlowast
1408518lowastlowast
10612519lowastlowast
077429lowastlowast
Hybrid
s44
10569731lowastlowast
2127302lowastlowast
2910
2537lowastlowast
19429240lowastlowast
329256lowastlowast
7000
03lowastlowast
186026552lowastlowast
1483428lowastlowast
Parentsv
ersush
ybrid
s1
16223710lowastlowast
25488361lowastlowast
1113
326
13257362lowastlowast
15089673lowastlowast
5178
3569lowastlowast
5634825791lowastlowast
51618348lowastlowast
Error
108
334760
1097039
1049505
635570
06764
116
0576
694613
006206
E 3
Replicates
2894398
4808815lowast
mdashmdash
051076
095989
3768421
030220
Treatm
ents
541261560
0lowastlowast
4842216lowastlowast
mdashmdash
2811077lowastlowast
5947316lowastlowast
3870844
50lowastlowast
3445076lowastlowast
Parents
97135
735lowastlowast
1097
6388lowastlowast
mdashmdash
1468699lowastlowast
1112
764lowastlowast
136081650lowastlowast
1307836lowastlowast
Hybrid
s44
13217740lowastlowast
1379360
mdashmdash
15540
71lowastlowast
4581632lowastlowast
252084767lowastlowast
2378032lowastlowast
Parentsv
ersush
ybrid
s1
35440
180lowastlowast
102000335lowastlowast
mdashmdash
70200767lowastlowast
109548362lowastlowast
8586095705lowastlowast
69630188lowastlowast
Error
108
782907
1085444
mdashmdash
139771
291325
3019
169
02400
4(b)
Environm
ent
Source
ofvaria
tion
DF
Meansum
ofsquares
Panicle
emergence
Effectiv
etillersplant
Planth
eight
Earlength
Earw
eightp
lant
Dry
fodd
eryieldplant
Grain
yieldplant
Totalbiological
yieldplant
E 1
Replicates
231860
61lowastlowast
416348lowastlowast
1672
9472lowast
1706
411077192
9634539
392777
115544
59Treatm
ents
544152
368lowastlowast
085657lowastlowast
326706089lowastlowast
1602572lowastlowast
6177
0406lowastlowast
304113
204lowastlowast
34285030lowastlowast
575617
582lowastlowast
Parents
94511481lowastlowast
018538lowastlowast
140807476lowastlowast
2240093lowastlowast
11653
959lowastlowast
27014155lowastlowast
3858650lowastlowast
61321919lowastlowast
Hybrid
s44
3201347lowastlowast
100797lowastlowast
110395657lowastlowast
1010
497lowastlowast
40296671lowastlowast
171271411lowastlowast
2413
6880lowastlowast
302999650lowastlowast
Parentsv
ersush
ybrid
s1
4276
5286lowastlowast
02360
9lowast11517452
616lowastlowast
2191
6167lowastlowast
1457662749lowastlowast
8643043524lowastlowast
7546
41053lowastlowast
17199467546lowastlowast
Error
108
468159
004907
3692099
224610
17114
303498126
682350
5075268
E 2
Replicates
2656364
002556
61575565lowastlowast
828842lowastlowast
589094
2268506
260877
1507116
Treatm
ents
542999618lowastlowast
069822lowastlowast
268998723lowastlowast
2436886lowastlowast
60592784lowastlowast
247259877lowastlowast
4192
0073lowastlowast
513483489lowastlowast
Parents
926300
00lowastlowast
048079lowastlowast
165555
405lowastlowast
3871874lowastlowast
1412
2393lowastlowast
19543682lowastlowast
3132
426lowastlowast
57230706lowastlowast
Hybrid
s44
2533
165lowastlowast
07344
9lowastlowast
9616
2857lowastlowast
1463359lowastlowast
35495366lowastlowast
154759288lowastlowast
26262465lowastlowast
293492304lowastlowast
Parentsv
ersush
ybrid
s1
26850135lowastlowast
105929lowastlowast
880476
6697lowastlowast
32357161lowastlowast
1583112746lowastlowast
6366731560lowastlowast
107994
3646lowastlowast
14299370699lowastlowast
Error
108
507598
005829
5234122
149406
955133
2142411
462311
3273886
E 3
Replicates
2029697
021161
9459847
143746
985141
46800
6492
6334
3345160
Treatm
ents
548045499lowastlowast
094511lowastlowast
192420108lowastlowast
3279225lowastlowast
72049211lowastlowast
298953
021lowastlowast
55061308lowastlowast
651732994lowastlowast
Parents
98010
741lowastlowast
094166lowastlowast
116037343lowastlowast
3702185lowastlowast
16305190lowastlowast
2323644
0lowastlowast
4553
893lowastlowast
71484982lowastlowast
Hybrid
s44
6254377lowastlowast
095514lowastlowast
7378
1513lowastlowast
2188348lowastlowast
31813193lowastlowast
190263916lowastlowast
31572179lowastlowast
368689934lowastlowast
Parentsv
ersush
ybrid
s1
8716
7710lowastlowast
053496lowast
609996
3199lowastlowast
47471212lowastlowast
2344
130203lowastlowast
756272
2861lowastlowast
154314
9707lowastlowast
18327859
719lowastlowast
Error
108
696981
007883
5578505
228608
1491014
2574
223
449588
5187705
lowast
Sign
ificant
at119875=005
lowastlowastSign
ificant
at119875=001
14 Advances in Agriculture
Table 5 Mean performance of the genotypes for membrane thermostability in two environments
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594
Advances in Agriculture 15
Table 5 Continued
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434
Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash
lowastValues are transformed values
0
6
12
18
24
30
36
42
48
54
minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity
G73-10796AC-93CVJ-2-5-3-1-3
Togo-II99HS-18
H77833-2H7729-2772451305
H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305
1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305
H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305
77245 times 1305
77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305
77371 times BSECT CP-1
H77833-2 times G73-107
Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits
moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling
survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The
16 Advances in Agriculture
Table6Po
oled
correla
tioncoeffi
cientamon
gvario
uscharactersin
threee
nviro
nments
Characters
Germination
Rateof
emergence
STI
SSTI
Leaves
seedlin
gSeedlin
gheight
Fresh
weight
Dry
weight
MTS
Panicle
emergence
Effectiv
etillersplant
Plant
height
Earlength
Ear
weightp
lant
Dry
fodd
eryield
plant
Grain
yield
plant
Total
biological
yield
plant
Germination
1000
0357lowastlowast
0353lowastlowast
0590lowastlowast
0230
0373lowastlowast
0293lowast
0260
0276lowastminus0135minus0478lowastlowast
0326lowast
minus0069minus0104
minus0190
minus0145
minus0167
Rateof
emergence
1000
0233
0351lowastlowast
0605lowastlowast
0585lowastlowast
0551lowastlowast
0524lowastlowast
0139minus0593lowastlowast
0032
0517lowastlowast
0217
0388lowastlowast
0361lowastlowast
0348lowastlowast
0377lowastlowast
STI
1000
0837lowastlowast
0241
0129
0081
004
80890lowastlowast
minus0215
minus0211
0030minus0007minus0043
minus0101
minus0111
minus0084
SSTI
1000
0233
0264
0183
0140
0745lowastlowast
minus0247minus0268lowast
0184minus0017minus006
6minus0129
minus0144
minus0112
Num
bero
fleaves
1000
0683lowastlowast
0633lowastlowast
060
4lowastlowast
0176minus0474lowastlowast
0109
0576lowastlowast
0496lowastlowast
064
1lowastlowast
0607lowastlowast
0630lowastlowast
0630lowastlowast
Seedlin
gheight
1000
0703lowastlowast
0663lowastlowast
0031minus0493lowastlowast
minus0115
0735lowastlowast
0360lowastlowast
0526lowastlowast
0520lowastlowast
0515lowastlowast
0532lowastlowast
Freshweight
1000
0957lowastlowast
minus0016
0425lowastlowast
minus0082
0661lowastlowast
0351lowastlowast
0581lowastlowast
0496lowastlowast
0576lowastlowast
0533lowastlowast
Dry
weight
1000minus0033minus0428lowastlowast
minus0016
0634lowastlowast
0354lowastlowast
0620lowastlowast
0509lowastlowast
0614lowastlowast
0554lowastlowast
MTS
1000minus040
minus0177minus0037
0015
minus0074
minus0138
minus0131
minus0120
Panicle
emergence
1000
minus0150minus0269lowast
0022minus0329lowast
minus0337lowast
minus0280lowast
minus0341lowast
Effectiv
etillersplant
1000
minus0012
0170
0366lowastlowast
040
5lowastlowast
0323lowast
0401lowastlowast
Planth
eight
1000
0650lowastlowast
0603lowastlowast
0596lowastlowast
0619lowastlowast
0610lowastlowast
Earlength
1000
0608lowastlowast
0671lowastlowast
064
8lowastlowast
066
4lowastlowast
Earw
eightp
lant
1000
0910lowastlowast
0984lowastlowast
0957lowastlowast
Dry
fodd
eryield
plant
1000
0907lowastlowast
0991lowastlowast
Grain
yield
plant
1000
0950lowastlowast
Totalbiological
yield
plant
1000
lowastSign
ificant
at119875=005lowastlowastSign
ificant
at119875=001
Advances in Agriculture 17
faster germination and early vigour in seedling could act asphysical indicators for heat tolerance
Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits
Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics
Conflict of Interests
The authors declare that there is no conflict of interestsregarding the publication of this paper
References
[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981
[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993
[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006
[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993
[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013
[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007
[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965
[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011
[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001
[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009
[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962
[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967
[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963
[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001
Submit your manuscripts athttpwwwhindawicom
Nutrition and Metabolism
Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Food ScienceInternational Journal of
Agronomy
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
Microbiology
The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom
Applied ampEnvironmentalSoil Science
Volume 2014
AgricultureAdvances in
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
BiodiversityInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
GenomicsInternational Journal of
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Plant GenomicsInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Biotechnology Research International
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Forestry ResearchInternational Journal of
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Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
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Veterinary Medicine International
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Cell BiologyInternational Journal of
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Evolutionary BiologyInternational Journal of
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14 Advances in Agriculture
Table 5 Mean performance of the genotypes for membrane thermostability in two environments
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594
Advances in Agriculture 15
Table 5 Continued
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434
Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash
lowastValues are transformed values
0
6
12
18
24
30
36
42
48
54
minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity
G73-10796AC-93CVJ-2-5-3-1-3
Togo-II99HS-18
H77833-2H7729-2772451305
H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305
1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305
H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305
77245 times 1305
77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305
77371 times BSECT CP-1
H77833-2 times G73-107
Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits
moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling
survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The
16 Advances in Agriculture
Table6Po
oled
correla
tioncoeffi
cientamon
gvario
uscharactersin
threee
nviro
nments
Characters
Germination
Rateof
emergence
STI
SSTI
Leaves
seedlin
gSeedlin
gheight
Fresh
weight
Dry
weight
MTS
Panicle
emergence
Effectiv
etillersplant
Plant
height
Earlength
Ear
weightp
lant
Dry
fodd
eryield
plant
Grain
yield
plant
Total
biological
yield
plant
Germination
1000
0357lowastlowast
0353lowastlowast
0590lowastlowast
0230
0373lowastlowast
0293lowast
0260
0276lowastminus0135minus0478lowastlowast
0326lowast
minus0069minus0104
minus0190
minus0145
minus0167
Rateof
emergence
1000
0233
0351lowastlowast
0605lowastlowast
0585lowastlowast
0551lowastlowast
0524lowastlowast
0139minus0593lowastlowast
0032
0517lowastlowast
0217
0388lowastlowast
0361lowastlowast
0348lowastlowast
0377lowastlowast
STI
1000
0837lowastlowast
0241
0129
0081
004
80890lowastlowast
minus0215
minus0211
0030minus0007minus0043
minus0101
minus0111
minus0084
SSTI
1000
0233
0264
0183
0140
0745lowastlowast
minus0247minus0268lowast
0184minus0017minus006
6minus0129
minus0144
minus0112
Num
bero
fleaves
1000
0683lowastlowast
0633lowastlowast
060
4lowastlowast
0176minus0474lowastlowast
0109
0576lowastlowast
0496lowastlowast
064
1lowastlowast
0607lowastlowast
0630lowastlowast
0630lowastlowast
Seedlin
gheight
1000
0703lowastlowast
0663lowastlowast
0031minus0493lowastlowast
minus0115
0735lowastlowast
0360lowastlowast
0526lowastlowast
0520lowastlowast
0515lowastlowast
0532lowastlowast
Freshweight
1000
0957lowastlowast
minus0016
0425lowastlowast
minus0082
0661lowastlowast
0351lowastlowast
0581lowastlowast
0496lowastlowast
0576lowastlowast
0533lowastlowast
Dry
weight
1000minus0033minus0428lowastlowast
minus0016
0634lowastlowast
0354lowastlowast
0620lowastlowast
0509lowastlowast
0614lowastlowast
0554lowastlowast
MTS
1000minus040
minus0177minus0037
0015
minus0074
minus0138
minus0131
minus0120
Panicle
emergence
1000
minus0150minus0269lowast
0022minus0329lowast
minus0337lowast
minus0280lowast
minus0341lowast
Effectiv
etillersplant
1000
minus0012
0170
0366lowastlowast
040
5lowastlowast
0323lowast
0401lowastlowast
Planth
eight
1000
0650lowastlowast
0603lowastlowast
0596lowastlowast
0619lowastlowast
0610lowastlowast
Earlength
1000
0608lowastlowast
0671lowastlowast
064
8lowastlowast
066
4lowastlowast
Earw
eightp
lant
1000
0910lowastlowast
0984lowastlowast
0957lowastlowast
Dry
fodd
eryield
plant
1000
0907lowastlowast
0991lowastlowast
Grain
yield
plant
1000
0950lowastlowast
Totalbiological
yield
plant
1000
lowastSign
ificant
at119875=005lowastlowastSign
ificant
at119875=001
Advances in Agriculture 17
faster germination and early vigour in seedling could act asphysical indicators for heat tolerance
Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits
Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics
Conflict of Interests
The authors declare that there is no conflict of interestsregarding the publication of this paper
References
[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981
[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993
[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006
[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993
[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013
[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007
[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965
[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011
[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001
[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009
[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962
[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967
[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963
[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001
Submit your manuscripts athttpwwwhindawicom
Nutrition and Metabolism
Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Food ScienceInternational Journal of
Agronomy
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
Microbiology
The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom
Applied ampEnvironmentalSoil Science
Volume 2014
AgricultureAdvances in
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
BiodiversityInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
GenomicsInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Plant GenomicsInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Biotechnology Research International
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Forestry ResearchInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
EcologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Veterinary Medicine International
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Cell BiologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Evolutionary BiologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Advances in Agriculture 15
Table 5 Continued
Sr No Genotypes Membrane thermostabilitylowast
E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434
Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash
lowastValues are transformed values
0
6
12
18
24
30
36
42
48
54
minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity
G73-10796AC-93CVJ-2-5-3-1-3
Togo-II99HS-18
H77833-2H7729-2772451305
H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305
1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305
H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305
77245 times 1305
77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305
77371 times BSECT CP-1
H77833-2 times G73-107
Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits
moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling
survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The
16 Advances in Agriculture
Table6Po
oled
correla
tioncoeffi
cientamon
gvario
uscharactersin
threee
nviro
nments
Characters
Germination
Rateof
emergence
STI
SSTI
Leaves
seedlin
gSeedlin
gheight
Fresh
weight
Dry
weight
MTS
Panicle
emergence
Effectiv
etillersplant
Plant
height
Earlength
Ear
weightp
lant
Dry
fodd
eryield
plant
Grain
yield
plant
Total
biological
yield
plant
Germination
1000
0357lowastlowast
0353lowastlowast
0590lowastlowast
0230
0373lowastlowast
0293lowast
0260
0276lowastminus0135minus0478lowastlowast
0326lowast
minus0069minus0104
minus0190
minus0145
minus0167
Rateof
emergence
1000
0233
0351lowastlowast
0605lowastlowast
0585lowastlowast
0551lowastlowast
0524lowastlowast
0139minus0593lowastlowast
0032
0517lowastlowast
0217
0388lowastlowast
0361lowastlowast
0348lowastlowast
0377lowastlowast
STI
1000
0837lowastlowast
0241
0129
0081
004
80890lowastlowast
minus0215
minus0211
0030minus0007minus0043
minus0101
minus0111
minus0084
SSTI
1000
0233
0264
0183
0140
0745lowastlowast
minus0247minus0268lowast
0184minus0017minus006
6minus0129
minus0144
minus0112
Num
bero
fleaves
1000
0683lowastlowast
0633lowastlowast
060
4lowastlowast
0176minus0474lowastlowast
0109
0576lowastlowast
0496lowastlowast
064
1lowastlowast
0607lowastlowast
0630lowastlowast
0630lowastlowast
Seedlin
gheight
1000
0703lowastlowast
0663lowastlowast
0031minus0493lowastlowast
minus0115
0735lowastlowast
0360lowastlowast
0526lowastlowast
0520lowastlowast
0515lowastlowast
0532lowastlowast
Freshweight
1000
0957lowastlowast
minus0016
0425lowastlowast
minus0082
0661lowastlowast
0351lowastlowast
0581lowastlowast
0496lowastlowast
0576lowastlowast
0533lowastlowast
Dry
weight
1000minus0033minus0428lowastlowast
minus0016
0634lowastlowast
0354lowastlowast
0620lowastlowast
0509lowastlowast
0614lowastlowast
0554lowastlowast
MTS
1000minus040
minus0177minus0037
0015
minus0074
minus0138
minus0131
minus0120
Panicle
emergence
1000
minus0150minus0269lowast
0022minus0329lowast
minus0337lowast
minus0280lowast
minus0341lowast
Effectiv
etillersplant
1000
minus0012
0170
0366lowastlowast
040
5lowastlowast
0323lowast
0401lowastlowast
Planth
eight
1000
0650lowastlowast
0603lowastlowast
0596lowastlowast
0619lowastlowast
0610lowastlowast
Earlength
1000
0608lowastlowast
0671lowastlowast
064
8lowastlowast
066
4lowastlowast
Earw
eightp
lant
1000
0910lowastlowast
0984lowastlowast
0957lowastlowast
Dry
fodd
eryield
plant
1000
0907lowastlowast
0991lowastlowast
Grain
yield
plant
1000
0950lowastlowast
Totalbiological
yield
plant
1000
lowastSign
ificant
at119875=005lowastlowastSign
ificant
at119875=001
Advances in Agriculture 17
faster germination and early vigour in seedling could act asphysical indicators for heat tolerance
Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits
Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics
Conflict of Interests
The authors declare that there is no conflict of interestsregarding the publication of this paper
References
[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981
[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993
[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006
[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993
[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013
[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007
[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965
[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011
[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001
[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009
[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962
[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967
[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963
[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001
Submit your manuscripts athttpwwwhindawicom
Nutrition and Metabolism
Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Food ScienceInternational Journal of
Agronomy
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
Microbiology
The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom
Applied ampEnvironmentalSoil Science
Volume 2014
AgricultureAdvances in
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
BiodiversityInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
GenomicsInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Plant GenomicsInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Biotechnology Research International
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Forestry ResearchInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
EcologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Veterinary Medicine International
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Cell BiologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Evolutionary BiologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
16 Advances in Agriculture
Table6Po
oled
correla
tioncoeffi
cientamon
gvario
uscharactersin
threee
nviro
nments
Characters
Germination
Rateof
emergence
STI
SSTI
Leaves
seedlin
gSeedlin
gheight
Fresh
weight
Dry
weight
MTS
Panicle
emergence
Effectiv
etillersplant
Plant
height
Earlength
Ear
weightp
lant
Dry
fodd
eryield
plant
Grain
yield
plant
Total
biological
yield
plant
Germination
1000
0357lowastlowast
0353lowastlowast
0590lowastlowast
0230
0373lowastlowast
0293lowast
0260
0276lowastminus0135minus0478lowastlowast
0326lowast
minus0069minus0104
minus0190
minus0145
minus0167
Rateof
emergence
1000
0233
0351lowastlowast
0605lowastlowast
0585lowastlowast
0551lowastlowast
0524lowastlowast
0139minus0593lowastlowast
0032
0517lowastlowast
0217
0388lowastlowast
0361lowastlowast
0348lowastlowast
0377lowastlowast
STI
1000
0837lowastlowast
0241
0129
0081
004
80890lowastlowast
minus0215
minus0211
0030minus0007minus0043
minus0101
minus0111
minus0084
SSTI
1000
0233
0264
0183
0140
0745lowastlowast
minus0247minus0268lowast
0184minus0017minus006
6minus0129
minus0144
minus0112
Num
bero
fleaves
1000
0683lowastlowast
0633lowastlowast
060
4lowastlowast
0176minus0474lowastlowast
0109
0576lowastlowast
0496lowastlowast
064
1lowastlowast
0607lowastlowast
0630lowastlowast
0630lowastlowast
Seedlin
gheight
1000
0703lowastlowast
0663lowastlowast
0031minus0493lowastlowast
minus0115
0735lowastlowast
0360lowastlowast
0526lowastlowast
0520lowastlowast
0515lowastlowast
0532lowastlowast
Freshweight
1000
0957lowastlowast
minus0016
0425lowastlowast
minus0082
0661lowastlowast
0351lowastlowast
0581lowastlowast
0496lowastlowast
0576lowastlowast
0533lowastlowast
Dry
weight
1000minus0033minus0428lowastlowast
minus0016
0634lowastlowast
0354lowastlowast
0620lowastlowast
0509lowastlowast
0614lowastlowast
0554lowastlowast
MTS
1000minus040
minus0177minus0037
0015
minus0074
minus0138
minus0131
minus0120
Panicle
emergence
1000
minus0150minus0269lowast
0022minus0329lowast
minus0337lowast
minus0280lowast
minus0341lowast
Effectiv
etillersplant
1000
minus0012
0170
0366lowastlowast
040
5lowastlowast
0323lowast
0401lowastlowast
Planth
eight
1000
0650lowastlowast
0603lowastlowast
0596lowastlowast
0619lowastlowast
0610lowastlowast
Earlength
1000
0608lowastlowast
0671lowastlowast
064
8lowastlowast
066
4lowastlowast
Earw
eightp
lant
1000
0910lowastlowast
0984lowastlowast
0957lowastlowast
Dry
fodd
eryield
plant
1000
0907lowastlowast
0991lowastlowast
Grain
yield
plant
1000
0950lowastlowast
Totalbiological
yield
plant
1000
lowastSign
ificant
at119875=005lowastlowastSign
ificant
at119875=001
Advances in Agriculture 17
faster germination and early vigour in seedling could act asphysical indicators for heat tolerance
Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits
Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics
Conflict of Interests
The authors declare that there is no conflict of interestsregarding the publication of this paper
References
[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981
[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993
[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006
[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993
[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013
[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007
[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965
[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011
[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001
[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009
[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962
[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967
[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963
[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001
Submit your manuscripts athttpwwwhindawicom
Nutrition and Metabolism
Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Food ScienceInternational Journal of
Agronomy
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
Microbiology
The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom
Applied ampEnvironmentalSoil Science
Volume 2014
AgricultureAdvances in
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
BiodiversityInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
GenomicsInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Plant GenomicsInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Biotechnology Research International
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Forestry ResearchInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
EcologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Veterinary Medicine International
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Cell BiologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Evolutionary BiologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Advances in Agriculture 17
faster germination and early vigour in seedling could act asphysical indicators for heat tolerance
Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits
Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics
Conflict of Interests
The authors declare that there is no conflict of interestsregarding the publication of this paper
References
[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981
[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993
[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006
[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993
[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013
[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007
[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965
[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011
[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001
[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009
[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962
[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967
[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963
[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001
Submit your manuscripts athttpwwwhindawicom
Nutrition and Metabolism
Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Food ScienceInternational Journal of
Agronomy
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
Microbiology
The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom
Applied ampEnvironmentalSoil Science
Volume 2014
AgricultureAdvances in
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
BiodiversityInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
GenomicsInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Plant GenomicsInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Biotechnology Research International
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Forestry ResearchInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
EcologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Veterinary Medicine International
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Cell BiologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Evolutionary BiologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Submit your manuscripts athttpwwwhindawicom
Nutrition and Metabolism
Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Food ScienceInternational Journal of
Agronomy
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
International Journal of
Microbiology
The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014
Hindawi Publishing Corporationhttpwwwhindawicom
Applied ampEnvironmentalSoil Science
Volume 2014
AgricultureAdvances in
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
BiodiversityInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
GenomicsInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Plant GenomicsInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Biotechnology Research International
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Forestry ResearchInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014
EcologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Veterinary Medicine International
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Cell BiologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014
Evolutionary BiologyInternational Journal of
Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014