research article screening of pearl millet f hybrids for...

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Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance at Early Seedling Stage Ashok Kumar Yadav, 1,2 Rajesh Kumar Arya, 2 and M. S. Narwal 2 1 CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh 176061, India 2 Department of Genetics and Plant Breeding, CCS HAU, Hisar 125004, India Correspondence should be addressed to Ashok Kumar Yadav; [email protected] Received 30 April 2014; Revised 7 August 2014; Accepted 26 August 2014; Published 2 October 2014 Academic Editor: Clifford Gold Copyright © 2014 Ashok Kumar Yadav et al. is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Ten pearl millet genotypes selected on the basis of response to supra-optimal temperature tolerance were crossed in a half-diallel mating system. e 45 F 1 hybrids produced were tested along with parents for heat tolerance and related traits at seedling stage. Field screening and laboratory screening techniques were simultaneously used for the evaluation of F 1 hybrids and their parents. Heat tolerance was measured as seedling thermotolerance index (STI) and seed to seedling thermotolerance index (SSTI) under field conditions, but membrane thermostability (MTS) in the laboratory. e hybrid H77/29-2 × CVJ-2-5-3-1-3 showed highest STI value followed by H77/833-2 × 96AC-93. e genotype H77/833-2 × 96AC-93 had the highest worth for SSTI. ese three indices were highly correlated among themselves. STI values were invariably high, whereas SSTI has lower values, as it also covers the effect of under soil mortality (USM). It was seen that the heat tolerance indices STI and SSTI were not showing any perceptible pooled correlation with developmental traits except germination and emergence rate. Based on our results, it could be suggested that membrane thermostability (MTS) may be used for screening large number of genotypes. Field based indices STI and SSTI may be used for evaluation of hybrids and varieties before they are released. 1. Introduction Pearl millet [Pennisetum glaucum (L.) R. Br.] is a multipur- pose cereal grown for grain, stover, and green fodder. It is the most important staple crop in the semiarid and arid regions of Asia and sub-Saharan Africa. It shall continue to play a prominent role in the integrated agricultural and livestock economy of the country particularly in rainfed areas due to its drought hardiness. Pearl millet provides an excellent nutritious food because of high biological value, more protein (11.6%), fat (5%), and mineral content (2.3%). It also contains adequate amount of essential amino acids such as tryptophan, threonine, arginine, and lysine. Pearl millet is also high in lipids and calcium and low in crude fibre. Adequate crop stand establishment problems are severe constraints to get good production of pearl millet. Failure to obtain adequate plant populations is oſten associated with high temperatures occurring during the germination and seedling establishment period of pearl millet. e temper- ature is one of the key climatic factors and has profound effect on the growth and development of the pearl millet. e soil temperatures in farmers’ field in India and Africa commonly exceed 45 C and the temperatures as high as 60 C have occasionally been measured [13]. e supraoptimal temperatures are known to be the most important cause of poor crop stands in farmer’s field. is can only be managed through developing hybrid varieties which can tolerate high temperature during germination and early seedling stages. Genetic variability for adaptation to high temperature exists in crop plants. Screening of F 1 crosses and identification of the superior cross combinations for seedling heat tolerance is essential for effective manipulation through hybrid breeding. e objective of this study was to understand the response of different cross combinations for heat tolerance during Hindawi Publishing Corporation Advances in Agriculture Volume 2014, Article ID 231301, 17 pages http://dx.doi.org/10.1155/2014/231301

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Page 1: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

Research ArticleScreening of Pearl Millet F1 Hybrids for Heat Tolerance atEarly Seedling Stage

Ashok Kumar Yadav12 Rajesh Kumar Arya2 and M S Narwal2

1 CSIR-Institute of Himalayan Bioresource Technology Palampur Himachal Pradesh 176061 India2Department of Genetics and Plant Breeding CCS HAU Hisar 125004 India

Correspondence should be addressed to Ashok Kumar Yadav ashokihbtresin

Received 30 April 2014 Revised 7 August 2014 Accepted 26 August 2014 Published 2 October 2014

Academic Editor Clifford Gold

Copyright copy 2014 Ashok Kumar Yadav et al This is an open access article distributed under the Creative Commons AttributionLicense which permits unrestricted use distribution and reproduction in any medium provided the original work is properlycited

Ten pearl millet genotypes selected on the basis of response to supra-optimal temperature tolerance were crossed in a half-diallelmating system The 45 F

1hybrids produced were tested along with parents for heat tolerance and related traits at seedling stage

Field screening and laboratory screening techniques were simultaneously used for the evaluation of F1hybrids and their parents

Heat tolerance was measured as seedling thermotolerance index (STI) and seed to seedling thermotolerance index (SSTI) underfield conditions but membrane thermostability (MTS) in the laboratory The hybrid H7729-2 times CVJ-2-5-3-1-3 showed highestSTI value followed by H77833-2 times 96AC-93 The genotype H77833-2 times 96AC-93 had the highest worth for SSTI These threeindices were highly correlated among themselves STI values were invariably high whereas SSTI has lower values as it also coversthe effect of under soil mortality (USM) It was seen that the heat tolerance indices STI and SSTI were not showing any perceptiblepooled correlation with developmental traits except germination and emergence rate Based on our results it could be suggestedthat membrane thermostability (MTS) may be used for screening large number of genotypes Field based indices STI and SSTI maybe used for evaluation of hybrids and varieties before they are released

1 Introduction

Pearl millet [Pennisetum glaucum (L) R Br] is a multipur-pose cereal grown for grain stover and green fodder It is themost important staple crop in the semiarid and arid regionsof Asia and sub-Saharan Africa It shall continue to play aprominent role in the integrated agricultural and livestockeconomy of the country particularly in rainfed areas dueto its drought hardiness Pearl millet provides an excellentnutritious food because of high biological valuemore protein(116) fat (5) and mineral content (23) It also containsadequate amount of essential amino acids such as tryptophanthreonine arginine and lysine Pearl millet is also high inlipids and calcium and low in crude fibre

Adequate crop stand establishment problems are severeconstraints to get good production of pearl millet Failure toobtain adequate plant populations is often associated with

high temperatures occurring during the germination andseedling establishment period of pearl millet The temper-ature is one of the key climatic factors and has profoundeffect on the growth and development of the pearl milletThe soil temperatures in farmersrsquo field in India and Africacommonly exceed 45∘C and the temperatures as high as 60∘Chave occasionally been measured [1ndash3] The supraoptimaltemperatures are known to be the most important cause ofpoor crop stands in farmerrsquos field This can only be managedthrough developing hybrid varieties which can tolerate hightemperature during germination and early seedling stagesGenetic variability for adaptation to high temperature existsin crop plants Screening of F

1crosses and identification of

the superior cross combinations for seedling heat tolerance isessential for effective manipulation through hybrid breedingThe objective of this study was to understand the responseof different cross combinations for heat tolerance during

Hindawi Publishing CorporationAdvances in AgricultureVolume 2014 Article ID 231301 17 pageshttpdxdoiorg1011552014231301

2 Advances in Agriculture

Table 1 Description of pearl millet genotypes used as parents for hybridization

Sr No Genotype Response Status Origin and characteristics

1 H77833-2 Tolerant Inbred Bred at CCSHAU Hisar by selfing and selection within a Rajasthanilandrace population

2 H7729-2 Susceptible InbredBred at CCSHAU Hisar It is medium dwarf nodalpigmentationmdashviolet brown resistant to downy mildew shoot flygrey weevil chaffer beetle and leaf roller

3 G73-107 Tolerant Inbred Bred at CCSHAU Hisar and has profuse tillering narrow leaves andtypical orange brown anthers

4 77245 Susceptible Inbred Bred at CCSHAU Hisar by selfing and selection It is late floweringand high tillering

5 CVJ-2-5-3-1-3 Tolerant Inbred Dwarf developed at ICRISAT Patancheru

6 1305 Susceptible Inbred Bred at CCSHAU Hisar late flowering medium dwarf semicompactearhead

7 (77371 times BSECT CP-1) Tolerant Inbred Bred at CCSHAU Hisar using BSECT CP-1 from ICRISATPatancheru as male parent

8 96AC-93 Tolerant Inbred Resistant to downy mildew bred at CCSHAU Hisar by selfing andselection

9 Togo-II Tolerant Inbred Selection from ICRISAT material-Togo bred at CCSHAU Hisar10 99HS-18 Tolerant Inbred Bred at CCS Haryana Agricultural University Hisar

Table 2 Details of different environments created through sowing dates

Environment Nature of environment Date of sowing Site Location

Environment-1 (E1) Heat stress May 21 Research area Department ofGenetics and Plant Breeding

CCS HAU Hisar Haryana

Environment-2 (E2) Heat stress June 13 Research area Department ofGenetics and Plant Breeding

CCS HAU Hisar Haryana

Environment-3 (E3)(during monsoon season)

Nonstress July 17 Research area Department ofGenetics and Plant Breeding

CCS HAU Hisar Haryana

germination and early seedling stages in pearl millet and toidentify some promising F

1hybrids improvement for this

trait particularly for commercial cultivation

2 Materials and Methods

21 Plant Material A field screening technique that isthermotolerance index [4] was applied to screen the pearlmillet F

1hybrids for heat tolerance at seedling stage This

technique could be used in the hottest months (May-June)only with no rainfall Since the technique was applicableto the very young seedlings of pearl millet it was also ofconcern to test the association of seedling thermotoleranceto other phenotypic characteristics of seedlings The mem-brane thermostability (based on quantification of electrolyteleakage) was also conducted for screening the pearl milletF1hybrids at seedling stage Ten pearl millet genotypes

namely H77833-2 H7729-2 G73-107 77245 CVJ-2-5-3-1-3 1305 77371 times BSECT CP-1 96AC-93 Togo-II and 99HS-18 were selected from the germplasm on the basis of differentresponse to supraoptimal temperature tolerance (Table 1) inseparate experiment [5] These were crossed in a half-diallelmating system (excluding reciprocals) The 45 F

1hybrids

produced were tested along with parents for heat toleranceand related traits at seedling stage

22 Experimental Site and Conditions of Growth The exper-iment was conducted at the research area of Departmentof Genetics and Plant Breeding Chaudhary Charan SinghHaryana Agricultural University Hisar (Lat 29∘ 101015840N Long75∘ 461015840E and 2152m above mean sea level) located insubtropical region of Haryana India The 45 F

1hybrids

produced were tested along with parents for heat toleranceand related traits in three replicated plots under supraop-timal temperature exposure at seedling stage in two stressenvironments (environment-1 and environment-2) createdthrough different dates of sowing on May 21 and June 13 (thehottest period in North India) respectively so as to conductthe experiment under different range of temperatures withno rainfall A presowing medium light irrigation was donethrough flooding and the field was precisely levelled beforesowing After sowing no further irrigation was given to thestressed environments (environment-1 and environment-2)One nonstress environment (Table 2) was created throughsowing on July 17 (normal sowing period during monsoon)Each genotype was grown in 3 rows each of 3m length spaced

Advances in Agriculture 3

0

10

20

30

40

50

60

70

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15DAS

E1

E2

E3

Tem

pera

ture

(∘C)

(a)

05

101520253035404550

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15DAS

E1

E2

E3

Tem

pera

ture

(∘C)

(b)

0

10

20

30

40

50

60

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16DAS

E1

E2

E3

Tem

pera

ture

(∘C)

(c)

02468

101214161820

1 3 5 7 9 11 13 15

Moi

sture

()

DAS

E1

E2

E3

Permanent wilting point

(d)

Figure 1 (a) Soil surface temperature (b) air temperature (c) temperature 5 cm above the soil surface and (d) soil moisture status in differentenvironments

30 cm apart with 10 cm plant to plant spacingThe seeds weresown manually

The soil of experimental field was sandy loam in textureThe water holding capacity field capacity and permanentwilting point of the experimental field were 40 17 and 6percent respectively [6] Irrigation was withheld during thecourse of experiment however drought stress-free condi-tions were ensured through monitoring at regular interval(on alternate days) by measuring the moisture status of thesoil by gravimetric method The soil samples of about 50 gwere taken up to 20 cmdepth from the experimental plotThemoisture content on dry weight basis may be calculated [7]using the following formula

Soil moisture ()

=

(Weight of wet soil minusWeight of dry soil)Weight of dry soil

times 100

(1)

The heat stress period was terminated by irrigating theexperimental field before reaching the soil moisture level atpermanent wilting point A condition of no drought wasmaintained in order to determine the exclusive effect of tem-perature at seedling stageThis was maintained by measuringthe moisture status of the soil by gravimetric method onalternate days The soil surface temperature was measuredby a soil thermometer at soil surface temperature at 5 cmabove the soil surface and air temperature at 1m above thesoil surface were recorded between 200 pm and 230 pmdaily (Figure 1)

Emphasis of observation is centred on germination sur-vival and mortality of the seedlings

23 Plant CharacteristicsScreening Techniques The empha-sis was given to the observations of germination survival andmortality of the seedlingsThe seedlings were inspected everyday in the morning The seedlings which had died due to

4 Advances in Agriculture

heat stress were noted in each genotype on daily basis Obser-vations continued till the surviving seedlingswere established(15 DAE) and there was no further mortality Moreover thesoil moisture was depleting near to the permanent wiltingpoint and drought stress could also come into play along withheat stress to further continue the experiment Since experi-ment proceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedlingsurvival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of heat stress at seedling stage was observed on latergrowth stages

231 Thermotolerance Characteristics For heat tolerancecharacteristics 3 indices namely seedling thermotoleranceindex (STI) [4] seed to seedling thermotolerance index(SSTI) [8] and membrane thermostability (MTS) wereapplied to evaluate the hybrids and parents STI was cal-culated as the ratio of number of seedlings survived to thenumber of seedling emergences expressed in percentageSeed to seedling thermotolerance index (SSTI) was calculatedas the ratio of seedling survival to the number of seedlingsexpected to emerge expressed in percentage [8] The SSTIis an extension of STI by taking expected germination intoaccount It was necessary to correct the effect of under soilmortality (USM) The loss of germination due to heat (ieunder soil mortality) can be measured only after a standardgermination test Therefore the germination of seed fromthe same lot under monsoon environment (main pearl milletgrowing season where no under soil mortality occurreddue to heat stress) was taken as expected germination forcalculation of SSTI

232 Membrane Thermostability These genotypes were alsoscreened through laboratory technique (membrane ther-mostability) [9 10] The young leaves of 21-day-old seedlingsof each genotype in three replication plots were used formembrane thermostability test A middle portion of 10 cmfrom each leaf was taken and the midrib was removed andtwo equal halves of the 10 cm each were made Each halfwas taken to provide replications Each leaf portion was cutinto pieces and thoroughly washed and rinsed with distilledwater two times to remove the electrolytes adhering to theplant tissue as well as electrolyte released from the cutting ofplant tissue and put them into test tubes After final rinsingtubes were drained maintaining sufficient water to preventdesiccation of plant material during heat treatment Thesetest tubes were covered with aluminium foil and incubated inwater bath at 55∘C for 15 minutes for giving heat treatmentAfter the heat treatment period 10mL of distilled waterwas added to the test tubes Then all the test tubes wereheld at 10∘C overnight to allow diffusion of electrolytes fromplant material Test tubes were brought out of refrigeratorand shaken well to mix the contents An initial electricalconductivity (EC) of both replications of each treatment (119879

1)

was determined with electrical conductivity meter Thenthese test tubes were autoclaved at 15 psi for 2 minutes forcomplete electrolyte leakage from the leaf samples These testtubes were then cooled at room temperature and contentsmixed and final EC was taken for all the treatments (119879

2)

Membrane thermostability (MTS) was calculated accordingto Ibrahim and Quick [9] and Yadav et al [10] as follows

Membrane Thermostability (MTS) = (1 minus1198791

1198792

) times 100

(2)

where 1198791is conductivity reading after heat treatment and 119879

2

is conductivity reading after autoclavingCompletely randomized design (CRD) for membrane

thermostabilitywas conducted to detect variations among thegenotypes

233 Morphological Characteristics The seedling growthcharacteristics namely germination () emergence rate(ER) number of leavesseedling (two-week stage) seedlingheight (two-week stage) seedling fresh weight (g) (four-week stage) and seedling dry weight (g) (four-week stage)were recorded The data for seedling height and number ofleaves per seedling was recorded on 10 random seedlings perplot Emergence rate (an index for speed of germination)can be used as a tool in pearl millet breeding programs forevaluation of seedling vigour Speed of germination is oneof the oldest concepts of seedling vigour The emergencerate (ER) was calculated by dividing the number of normalseedlings obtained at each counting by the number of daysseeds had been in the seed bed (DAS)The values obtained ateach count were then summed at the end of the germinationtest to obtain the emergence rate [11]

Emergence Rate (ER) =Seedling count

Days to first count+ sdot sdot sdot

+

Seedling countDays to final count

(3)

After termination of the heat stress on the seedlings byirrigating the field the experiment was continued to studythe effect of seedling heat stress on maturity traits on thesurvived plants The maturity traits observed were panicleemergence effective tillersplant plant height ear length earweightplant dry fodder yieldplant grain yieldplant andtotal biological yieldplant

24 Statistical Analysis Analysis of variance for randomizedblock design (RBD) for field traits that is germinationemergence rate number of leavesseedling seedling heightseedling fresh weight and seedling dry weight and com-pletely randomized design (CRD) for membrane thermosta-bility test was conducted under each environment on meanbasis [12] Analyses were done using the software OPSTATdeveloped at CCS HAU Hisar Correlations among the vari-ous characters studied were calculated to establish the natureand magnitude of associations among the traits [8 13] with

Advances in Agriculture 5

the software SPSS (SPSS Inc) Multivariate clustering wasdone based on paired group cluster method using similaritycoefficient under Past 140 software [14] Multivariate analysisbased on the Euclidean distancematrix was estimated amonggenotypes to distinguish genotypes from each other Thegrouping of genotypes was generated using paired groupcluster method

3 Results and Discussion

The excessive heat (soil temperature up to 630∘C in E2)

about 18∘C higher than the normal atmospheric temperature(Figures 1(a) and 1(b)) was responsible for mortality ofseedlings Many seedlings were found desiccated at the pointof contact besides the desiccation at the tipsThe temperaturearound the tip level (5 cm above the soil surface) also reachedup to 50∘C (Figure 1(c)) It could therefore be concluded thatthe seedlings had to survive in a really high temperature Themoisture level remained above the permanent wilting point(Figure 1(d)) therefore drought effect was not in the playThe heat tolerance was measured as STI (Table 3) throughthe field screening technique of Peacock et al [4] Anotherindex known as SSTI was also calculated as an improvementand extensionThe analysis of variance (RBD) for 8 charactersin 3 environments showed significant genotypic differencesNo variability was observed for STI and SSTI in E

3only In

this environment no seedling mortality was observed andthe germination was taken as standard The values for STIand SSTI were therefore equal and the highest for all thegenotypes in this environment and hence no variation wasobserved

The hybrids were evaluated on the basis of mean perfor-mance for various characters in each environment (Table 3)On the mean basis STI value ranged from 5332 to 8224with an average of 7072 The hybrid H7729-2 times CVJ-2-5-3-1-3 was the best scorer for heat tolerance followed byH77833-2 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II The hybrid H77833-2 times 1305 was found susceptible toheat stress Seed to seedling thermotolerance index (SSTI)which is expected to be more rigorous index deviated thevalues from STI according to variation in germination undernormal environment (E

3) as the SSTI also coversincludes

the under surface mortality (USM) during germinationThe reductions in values were not strictly proportional tothe STI The reduction is dependent on thermosensitivityof germination We also came across such hybrids whichshowed poor germination and high STI SSTI values rangedfrom 4605 to 7287 with an average of 6090 The genotypeH77833-2 times 96AC-93 had the highest worth for SSTI onmean basis followed byG73-107times (77371timesBSECTCP-1) andH77833-2 times (77371 times BSECT CP-1) (Table 3) The analysisof variance for ten different characters in three differentenvironments (two stressed and one normal) was carried outand the results showed significant genotypic differences forall the characters (Table 4)

A caution is needed for evaluating such genotypes underSTI The SSTI gave a highly reduced value putting thesecrosses in the susceptible list It may be concluded therefore

that SSTI is more dependable and desirable tool for screeningand evaluating the genotypes for heat tolerance The germi-nation was lower in E

2than in E

1 This period has the highest

range of temperature with a high average Genotype-wise thehighest germination (6209) was recorded for the genotypeH7729-2 times (77371 times BSECT CP-1) on mean basis whichgives a preliminary indication of resistance to heat while thegenotype 1times 6 had the lowest germination (3533)The hybridH77833-2 times G73-107 was adjudged as the fastest emergenceof seedlingsThe genotype (77371timesBSECTCP-1)times 96AC-93had the highest fresh weightseedlingThe genotype H7729-2 times Togo-II had the highest seedling dry weight E

1had lower

dry weightseedling than E2indicating the more heat stress

during the E1

31 Membrane Thermostability Membrane thermostability(MTS) has long been recognised as a parameter of heattolerance All the hybrids were put to membrane thermosta-bility test in the laboratory under standard protocol Acompletely randomised design (CRD) analysis was carriedout for membrane thermostability (MTS) The mean sum ofsquares due to genotypes was highly significant indicatingthat enough genetic variability was present for membranethermostability (MTS) in each environment which justifiedfurther analysis of the data The range of values lay between5073 and 7742 Among the parents H77833-2 G73-107CVJ-2-5-3-1-3 77371 times BSECT CP-1 96AC-93 Togo-II and99HS-18 were verified as resistant genotypes and H7729-2 77245 and 1305 genotypes were found susceptible asmentioned in Table 1 (on the basis of previous experiment)Among all the hybrids H7729-2 times CVJ-2-5-3-1-3 CVJ-2-5-3-1-3 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II werethree highly tolerant genotypes on the basis of membranethermostability (Table 5)

32 Correlations The pooled correlations among the charac-ters were calculated (Table 6) around heat tolerance indices(STI SSTI and membrane thermostability) with a view tofind whether there was any association of these characterswith seedling as well as maturity traits It was observedthat STI and SSTI recorded the highest significant cor-relation among themselves as they are related indices forheat tolerance Membrane thermostability also showed highsignificant correlation with STI as well as SSTI STI hada significant positive association with germination of seedonly among seedling and maturity traits studied SSTI inaddition was also positively correlated with germination andemergence rate and negatively associated with number ofeffective tillersplantMembrane thermostability also showedsignificant positive correlation with seed germination amongseedling and maturity traits studied While evaluating thediallel progenies it was also seen that the heat toleranceindices STI SSTI and membrane thermostability were notshowing any perceptible correlation with the rest of thedevelopmental traits (seedling andmaturity traits)The stressperiod was terminated by irrigating the field experiment after15 days fromdate of sowingwhen the seedlings get establishedand further mortality stopped due to heat Moreover the soil

6 Advances in Agriculture

Table3Perfo

rmance

ofthep

arentsandthec

rosses

ford

ifferentcharacters

(a)

Sr

No

Genotypes

Germination(

)Ra

teof

emergencelowast

STIlowast

()

SSTIlowast(

)Num

bero

fleavesseedlin

gE 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

3941

3737

6164

4614

4037

4109

4279

4142

7830

7387

9000

8072

4498

4133

9000

5877

661

502

800

654

2H7729-2

5513

4726

6092

5443

2815

4980

3258

3684

4835

4495

9000

6110

4441

3602

9000

5681

363

294

427

361

3G73-107

5037

4826

5918

5260

4534

4132

4481

4382

6187

5413

9000

6866

5213

4460

9000

6224

878

710

935

841

477245

4747

4624

5565

4978

3625

4604

4047

4092

4385

3580

9000

5655

3803

3040

9000

5281

377

292

437

369

5CV

J-2-5-3-1-3

4824

4832

6897

5518

5044

4907

4960

4970

7193

6813

9000

7669

4959

4785

9000

6248

802

580

923

768

61305

4326

3891

5404

4540

3376

5056

3573

4002

3903

3281

9000

5395

3211

2452

9000

4888

350

284

414

349

777371timesBS

ECTCP

-14747

4502

6320

5190

4934

5075

5065

5025

6952

6706

9000

7552

5056

4671

9000

6242

621

542

700

621

896AC

-93

4651

3818

5722

4730

4312

4915

4753

4660

6660

6001

9000

7220

5212

3920

9000

6044

701

590

899

730

9To

go-II

4613

3937

5276

4609

4245

4737

4797

4593

7198

6861

9000

7686

5980

4767

9000

6582

681

629

853

721

1099HS-18

4575

3823

6247

4882

4317

4659

4746

4574

7594

7319

9000

7971

5154

4172

9000

6109

745

554

915

738

1H77833-2timesH7729-2

5787

5295

6733

5938

4964

5015

4989

4989

6803

6504

9000

7436

5808

5134

9000

6647

711

585

780

692

2H77833-2timesG73-107

5472

4417

6405

5431

5448

5422

5239

5370

7231

6769

9000

7667

5980

4556

9000

6512

1101

705

1340

1049

3H77833-2times77245

4076

3707

5504

4429

5217

5446

5238

5300

6627

6224

9000

7284

4690

4037

9000

5909

740

525

965

744

4H77833-2timesCV

J-2-5-3-1-3

4843

4300

5856

5000

4862

4956

4913

4911

7061

6519

9000

7527

5575

4632

9000

6403

998

885

1258

1047

5H77833-2times1305

3296

2780

4521

3533

5076

4907

5238

5074

3732

3265

9000

5332

2765

2050

9000

4605

1192

934

1600

1242

6H77833-2times(77371times

BSEC

TCP

-1)5636

5539

6258

5811

4489

5219

5015

4908

6981

6482

9000

7488

6146

5680

9000

6942

898

726

1525

1050

7H77833-2times96AC

-93

5720

5515

6335

5857

4800

5186

5075

5021

7833

7786

9000

8206

6603

6256

9000

7287

1107

873

1193

1058

8H77833-2timesTo

go-II

4862

3453

5709

4675

5036

4829

4911

4925

6190

5500

9000

6897

5214

3339

9000

5851

979

670

1107

919

9H77833-2times99HS-18

4575

4418

5447

4813

5041

5074

5006

5040

5800

5073

9000

6625

4846

4133

9000

5993

978

733

1135

949

10H7729-2timesG73-107

5615

4962

6273

5617

5023

5535

5165

5241

6143

5501

9000

6881

5540

4441

9000

6327

1000

720

1363

1028

11H7729-2times77245

5472

5164

6281

5639

4745

5031

5028

4935

5572

5175

9000

6582

4901

4345

9000

6082

880

620

897

799

12H7729-2timesCV

J-2-5-3-1-3

5494

5124

6536

5718

4804

5316

4957

5026

7596

8076

9000

8224

6093

5680

9000

6924

981

932

1451

1121

13H7729-2times1305

3804

3826

5511

4380

3596

4724

4387

4236

6362

7075

9000

7479

4230

4517

9000

5916

926

725

1497

1049

14H7729-2times(77371times

BSEC

TCP

-1)

6369

4847

7412

6209

4847

5351

5246

5148

6377

6031

9000

7136

5683

4230

9000

6304

977

671

1380

1009

15H7729-2times96AC

-93

5314

4657

6702

5558

4514

5295

5102

4971

5612

4856

9000

6489

4613

3603

9000

5739

846

679

1397

974

16H7729-2timesTo

go-II

5827

5594

6651

6024

5072

5279

5271

5207

6563

5371

9000

6978

5763

4652

9000

6472

873

719

1040

877

17H7729-2times99HS-18

5741

4638

7918

6099

4847

5024

5021

4964

6179

5603

9000

6927

4882

3725

9000

5869

1012

730

1600

1114

18G73-107times77245

5115

4646

6103

5288

4977

5242

5450

5223

7006

6063

9000

7356

5685

4594

9000

6426

1021

790

1292

1034

19G73-107timesCV

J-2-5-3-1-3

5134

4894

6427

5485

4901

5317

5181

5133

6637

6124

9000

7254

5253

4710

9000

6321

1039

863

1254

1052

20G73-107times1305

4881

4561

7597

5680

4717

4791

4775

4761

6704

6380

9000

7361

4556

4115

9000

5890

1140

882

1657

1226

21G73-107times(77371times

BSEC

TCP

-1)

5636

5519

6283

5812

4986

5219

5203

5136

7990

6203

9000

7731

6620

5436

9000

7019

977

703

1395

1025

22G73-107times96AC

-93

5740

4510

6415

5555

5129

4982

5195

5102

6978

5556

9000

7178

6156

4019

9000

6392

1077

860

1264

1067

23G73-107timesTo

go-II

4460

4357

6044

4953

4396

5138

4951

4829

5279

4835

9000

6371

4018

3603

9000

5540

906

881

1189

992

24G73-107times99HS-18

5473

4356

6374

5401

5102

4955

5055

5037

6460

5197

9000

6886

5534

3704

9000

6079

1171

766

1635

1191

2577245timesCV

J-2-5-3-1-3

4766

4666

5921

5117

5170

4918

4900

4996

5928

5859

9000

6929

4785

4613

9000

6133

984

920

1194

1033

2677245times1305

4575

4060

6052

4895

5128

5297

5203

5210

5102

4990

9000

6364

3979

3464

9000

5481

1279

823

1550

1217

2777245times(77371times

BSEC

TCP

-1)

5017

4929

6022

5322

5108

5473

5274

5285

5308

5044

9000

6451

4498

4191

9000

5896

833

588

939

787

Advances in Agriculture 7

(a)Con

tinued

Sr

No

Genotypes

Germination(

)Ra

teof

emergencelowast

STIlowast

()

SSTIlowast(

)Num

bero

fleavesseedlin

gE 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

2877245times96AC

-93

5472

4705

622154

66

5135

5197

5006

5113

5247

4843

9000

6363

4708

3784

9000

5831

795

662

1058

838

2977245timesTo

go-II

5252

4492

5889

5211

5188

4861

5074

5041

4885

4263

9000

6049

4421

3379

9000

5600

977

751

1243

990

3077245times99HS-18

5740

4099

6872

5570

5249

4898

5258

5135

5628

4616

9000

6415

4863

3014

9000

5626

1045

863

1184

1031

31CV

J-2-5-3-1-3times1305

4364

3944

5700

4669

4974

4517

4650

4714

6097

5579

9000

6892

4594

3842

9000

5812

936

848

1403

1063

32CV

J-2-5-3-1-3times(77371times

BSEC

TCP

-1)4345

3808

6774

4976

5272

4996

5035

5101

6650

5766

9000

7139

4288

3381

9000

5556

800

712

1013

841

33CV

J-2-5-3-1-3times96AC

-93

4862

4197

5743

4934

4884

4980

5058

4974

7477

6760

9000

7745

5957

4709

9000

6555

933

738

1478

1050

34CV

J-2-5-3-1-3timesTo

go-II

4670

4343

6233

5082

4399

4813

4631

4614

5806

6614

9000

7140

4422

4479

9000

5967

963

855

1180

999

35CV

J-2-5-3-1-3times99HS-18

5493

5080

7273

5949

5276

4984

5162

5141

7322

6590

9000

7637

5534

4766

9000

6433

998

807

1338

1047

361305times(77371times

BSEC

TCP

-1)

4613

4539

5717

4956

4986

4955

5080

5007

5137

4870

9000

6336

4210

3936

9000

5715

863

630

913

802

371305times96AC

-93

5473

5177

6830

5827

5224

4779

4919

4974

7117

6965

9000

7694

5640

5220

9000

6620

875

694

1476

1015

381305timesTo

go-II

4230

3420

5262

4304

4309

456144

45

4438

5781

4373

9000

6385

4575

2887

9000

5487

637

566

794

666

391305times99HS-18

5433

4507

6947

5629

5225

4753

4991

4989

7287

6968

9000

7752

5576

4479

9000

6352

1115

818

1410

1114

40(77371times

BSEC

TCP

-1)times

96AC

-93

5595

4429

7461

5828

5216

4955

5121

5097

7087

6368

9000

7485

5432

4038

9000

6157

955

753

1462

1057

41(77371times

BSEC

TCP

-1)times

Togo-II

5252

4728

6106

5362

5492

4808

5132

5144

8025

7505

9000

8177

6310

5413

9000

6908

859

693

990

848

42(77371times

BSEC

TCP

-1)times

99HS-18

5595

4298

6295

5396

4729

4887

5031

4883

7366

6945

9000

7770

6312

4556

9000

6623

903

622

1125

883

4396AC

-93timesTo

go-II

5017

3977

6486

5160

5019

5195

5237

5150

5922

5486

9000

6803

4689

3484

9000

5724

1007

728

1167

967

4496AC

-93times99HS-18

5595

4812

6924

5777

5036

5385

5205

5209

7411

5378

9000

7263

5873

3979

9000

6284

1013

652

1334

1000

45To

go-IItimes

99HS-18

4670

4497

7293

5487

4718

4319

4796

4611

5688

5431

9000

6706

3980

3665

9000

5548

939

653

1462

1018

Mean

5043

4482

6271

5265

4792

498149

23

4899

6377

584190

00

7072

5079

4190

9000

6090

898

701

1168

922

SE097

106

161

mdash213

191

190

mdash19

818

7mdash

mdash14

914

6mdash

mdash036

047

068

mdashCD

272

296

453

mdash597

537

534

mdash555

525

mdashmdash

421

409

mdashmdash

103

133

192

mdashlowastTransfo

rmed

data

(b)

Sr

No

Genotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

908

785

2245

1313

859

1537

1924

1440

154

143

160

152

6267

4800

3833

4967

212

227

249

229

15644

15458

13267

1479

02

H7729-2

1125

1175

1789

1363

317

2400

2811

1843

093

191

252

179

6700

5333

5100

5711

185

210

234

210

18850

18565

15389

17601

3G73-107

1166

1475

1966

1536

943

1744

4945

2544

143

129

366

213

6700

5433

5300

5811

218

219

238

225

15009

1496

513503

14492

477245

1438

1373

2085

1632

607

1863

5957

2809

117

180

509

269

6100

4800

5100

5333

232

221

253

235

14036

13802

11742

1319

35

CVJ-2-5-3-1-3

1214

1437

1807

1486

1163

1610

4166

2313

171

141

407

240

6300

4933

4600

5278

239

227

270

245

1994

317261

1497

017391

61305

858

1137

1737

1244

540

967

4861

2123

125

088

417

210

6533

5033

4800

5456

244

213

257

238

15527

15405

12274

14402

8 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

777371timesBS

ECT

CP-1

1377

1487

1892

1585

928

2660

3330

2306

150

258

301

236

5733

4533

4700

4989

242

220

293

252

1513

814550

1315

014279

896AC

-93

1303

1259

1852

1471

1056

1445

5972

2825

185

147

586

306

5500

4833

3833

4722

256

304

318

293

15577

14032

10000

13203

9To

go-II

1056

1220

2272

1516

924

1466

9113

3834

146

129

883

386

6167

4533

4600

5100

185

149

124

153

14578

12073

10033

12228

1099HS-18

1277

1075

1808

1387

1241

2833

7070

3715

228

226

615

356

6100

5000

4167

5089

243

173

324

247

12627

10355

1017

311052

1H77833-2times

H7729-2

1432

1498

2233

1721

1350

4405

8373

4709

159

499

782

480

5767

4433

3800

4667309

290

248

282

21010

18071

15847

18309

2H77833-2times

G73-107

1675

1635

2205

1838

1236

3211

5789

3412

134

313

547

331

5833

4767

3833

4811

262

295

236

265

21253

20449

15808

1917

0

3H77833-2times

77245

1577

1522

2263

1787

1058

6933

8719

5570

255

553

782

530

5267

4633

4600

4833

273

201

265

246

2074

119511

15208

18487

4H77833-2times

CVJ-2-5-3-1-3

1791

1757

2165

1904

20218030

5276

5109

354

779

477

537

5533

4233

3767

4511

165

330

351

282

21827

20072

17110

19670

5H77833-2times1305

1700

1842

28412128

1427

6228

9134

5596

570

579

813

654

5800

4267

3333

4467340

426

451

406

19816

19444

16037

18432

6H77833-2times

(77371times

BSEC

TCP

-1)

1453

1515

3120

2029

789

465510428

5291

211

408

858

492

5233

4167

3267

4222

203

283

267

251

20677

1913

816470

1876

2

7H77833-2times

96AC

-93

1835

1783

2427

2015

2532

6649

1610

684

29

350

650

1473

824

5467

4233

3700

4467243

239

256

246

22278

20434

15735

19482

8H77833-2times

Togo-II

1564

15632500

1876

1882

4772

11660

6105

296

469

972

579

5667

4533

440048

67

169

260

211

213

2412

82375

619389

22424

9H77833-2times

99HS-18

159318002049

1814

1571

480214024

6799

203

544

1547

765

6033

460040

0048

78

261

225

256

247

23466

21081

1793

320

827

10H7729-2times

G73-107

14951568

2075

1713

959

5157

8517

4877

218

382

666

422

6133

5067

4833

5344

300

273

239

271

23651

2191

717083

20884

11H7729-2times77245

1557

1537

2613

1902

1851

5756

5729

4445366

562

524

484

5633

4800

4633

5022

133

233

297

221

1979

219914

15250

18319

12H7729-2times

CVJ-2-5-3-1-3

18442033

28932257

1651

806114885

8199

264

597

1054

638

6333

4600

4233

5056

170

211

217

199

24629

23339

1970

022556

13H7729-2times1305

1325

1677309920

34

1756

724314048

7682

318

635

1232

728

630046

67

3633

4867298

261

361

307

21666

19371

17422

19486

14H7729-2times(77371

timesBS

ECTCP

-1)

1958

1887

3105

2317

1118

4675

14411

6734

242

457

1318

672

5900

4500

3800

4733

164

209

240

204

23661

1995

419250

20955

15H7729-2times

96AC

-93

1512

1717

2779

2002266844

08

11393

6156

356

500

1211

689

5633

4533

4200

4789

221

195

282

232

2194

921635

17213

20266

Advances in Agriculture 9

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

16H7729-2times

Togo-II

1519

1738

2159

1805

2437

9867

1616

294

89

361

961

1527

950

6167

4700

4200

5022

234

272

230

246

26338

2414

520553

23678

17H7729-2times

99HS-18

1938

1787

3151

2292

1574

6218

11318

6370

290

606

956

617

6067

4767

406749

67

172

210

199

194

24689

21854

1778

921444

18G73-107times77245

1856

1813

2210

1960

2435

8550

7828

6271338

633

612

528

5500

4633

440048

44

199

255

307

254

1993

619277

16095

18436

19G73-107times

CVJ-2-5-3-1-3

1517

1769

2407

1897

1556

7728

9136

6140

239

876

917

677

5900

4300

3400

4533

233

266

315

272

23418

22333

19317

21689

20G73-107times1305

1217

1507

2832

1852

1777

5189

1114

960

38

289

588

1197

691

6733

5000

4233

5322

286

210

312

269

2078

81992

817067

19261

21G73-107times(77371

timesBS

ECTCP

-1)

18551946

2730

2177

1801

3482

10415

5233

239

277

753

423

5500

4467

3433

4467243

197

273

238

23817

20263

17425

20502

22G73-107times

96AC

-93

1718

1840

28612140

1345

9333

14669

8449

297

845

1278

807

5600

5033

4633

5089

270

243

300

271

28537

21461

16875

22291

23G73-107timesTo

go-II

168018083087

2192

1112

4678

9319

5036

186

473

985

548

6033

4767

4200

5000

210

209

231

217

24864

24609

21625

23699

24G73-107times99HS-18

1398

1675

3370

2148

2753

8672

11918

7781

362

691

981

678

566746

00

3500

4589

170

247

270

229

24677

20567

17855

21033

2577245times

CVJ-2-5-3-1-3

1500

1728

2203

1810

2282

4679

11492

6151

328

424

1116

623

6000

4333

420048

44300

231

370

300

21257

20497

1772

519826

2677245times1305

143815772080

1698

949

10439

12053

7814

121

915

992

676

5767

4800

3500

4689

168246

316

244

1978

91779

015517

17699

2777245times(77371times

BSEC

TCP

-1)

150516972846

2016

3222

8518

12621

8120

469

827

1141

812

5400

4367

3833

4533

196

225

242

221

2092

819485

1719

219202

2877245times96AC

-93

1653

1797

2117

1856

1934

6056

11543

6511

352

563

911

608

5333

4267

3833

4478

186

169

198

184

22003

19054

1676

719275

2977245timesTo

go-II

1723

1718

3163

2201

1749

8250

15443

8481229

806

1412

816

5967

4700

4067

4911

174

245

298

239

22075

23503

19875

21818

3077245times99HS-18

2016

1970

28542280

3217

13027

3531

6591395

965

285

549

5733

4467

3833

4678

148

228

209

195

2212

721462

1690

020

163

31CV

J-2-5-3-1-3times

1305

1358

1422

2179

1653

1254

2803

1019

44750

178

254

801

411

6033

4467

3367

4622

312

193

330

278

22269

19547

17250

19689

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

1335

1742

2400

1826

922

5226

12806

6318

236

458

1054

583

5633

4433

3600

4556

321

315

259

298

2210

419524

17087

19572

33CV

J-2-5-3-1-3times

96AC

-93

1552

16803260

2164

166710094

11941

7901250

974

1115

779

5533

4533

446748

44

170

202

317

230

2392

123317

19333

2219

0

34CV

J-2-5-3-1-3times

Togo-II

142216072638

1889

1012

4163

11061

5412

261

377

891

510

6167

4967

4533

5222

207

225

325

252

23590

22860

20278

22243

35CV

J-2-5-3-1-3times

99HS-18

1435

1600

2700

1912

3056

3237

7089

4461449

301

658

469

5700

44673800

4656

148

178

225

183

22444

20240

1696

719884

361305times(77371times

BSEC

TCP

-1)

1499

1767

2058

1774

1882

4665

7920

4822

356

426

724

502

566742673800

4578

271

242

305

273

22011

19295

16417

19241

371305times96AC

-93

16781605

2757

2014

1516

10037

11960

7838

311

11381284

911

560040

673400

4356

182

276

251

236

2396

519625

15800

1979

738

1305timesTo

go-II

1177

16802249

1702

902

4676

9388

4989

241

456

795

497

6533

5533

4733

5600

214

266

296

259

21894

20217

19222

20444

391305times99HS-18

1774

1767

2766

2102

2145

4628

10618

5797

301

447

991

580

5933

4500

3733

4722

128

142

180

150

2275

719413

16967

1971

2

40(77371times

BSEC

TCP

-1)times

96AC

-93

1748

1795

31712238

1537

12586

14379

9501

211

1104

1183

833

546744

00346744

44

127

201

226

185

23294

21089

17858

2074

7

10 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(

days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

41(77371times

BSEC

TCP

-1)times

Togo-II

19652007

2653

2208

2136

5601

9503

5747

315

635

1001

650

5533

4833

410048

22

213

241

286

247

22662

22094

17667

20808

42(77371times

BSEC

TCP

-1)times

99HS-18

15011687

2705

1964

1935

7253

11806

6998

314

704

1067

695

5467

4433

5133

5011

177

167

175

173

22305

19277

16392

19325

4396AC

-93timesTo

go-II1759

1618

2123

1834

1539

10347

12212

8033

325

907

1090

774

5900

450039674789

214

269

339

274

23252

21554

16875

20560

4496AC

-93times

99HS-18

1353

1618

2925

1966

3532

7223

11950

7569

440

654

1024

706

5600

4900

4533

5011

149

175

206

177

24494

20698

18111

2110

145

Togo-IItimes

99HS-18

1117

1278

2582

1659

3448

6764

11910

7374

477

767

1207

817

6000

5133

4333

5156

183

203

216

201

17683

15541

14333

15853

Mean

1513

1618

2492

1874

1656

5773

9854

5761

272

538

885

565

586846

534116

4879

218

233

268

240

21297

19547

16529

1912

4SE

101

073

098

mdash079

152

317

mdash014

014

028

mdash351

365

428

mdash036

039

046

mdash985

1173

1211

mdashCD

284

205

276

mdash221

427

890

mdash038

040

079

mdash12

513

015

2mdash

013

014

016

mdash351

418

431

mdash

(c)

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

1642

1667

1744

1684

1504

1349

1526

1460

2783

2405

2634

2607

836

745

908

830

4287

3754

4160

4067

2H7729-2

1693

1817

1950

1820

1012

859

1016

962

3433

2809

2591

2944

649

597

651

632

4445

3668

3607

3906

3G73-107

2000

2028

2077

2035

2280

1966

2312

2186

4695

4023

4229

4316

1281

1170

1463

1305

6975

5989

6541

6502

477245

1523

1630

1728

1627

1796

2489

2702

2329

5423

4851

4498

4924

748

1464

1743

1319

7219

7341

7200

7253

5CV

J-2-5-3-1-3

2282

2692

2787

2587

2411

2643

2901

2652

5857

4836

5024

5239

1206

1335

1769

1437

8268

7479

7925

7891

61305

1851

1951

2054

1952

2026

1763

1908

1899

4657

4414

4463

4511

1468

1336

1539

1448

6683

6177

6371

6410

777371timesBS

ECTCP

-11629

1645

1733

1669

1244

1692

1884

1607

4925

4183

4435

4515

625

1092

1483

1067

6169

5875

6319

6121

896AC

-93

1379

1519

1600

1499

2066

2942

3088

2698

4501

4095

4229

4275

984

1565

1678

1409

6567

7037

7316

6973

9To

go-II

1904

2057

2103

2021

2167

1844

2358

2123

4520

3446

3904

3957

1298

1138

1502

1313

6687

5290

6262

6080

1099HS-18

1475

1485

1633

1531

3180

2904

3382

3155

5633

4139

5205

4992

1656

1536

1879

1690

8813

7043

8587

8147

1H77833-2timesH7729-2

2010

2046

2133

2063

3645

3075

4377

3699

7675

6540

7199

7138

2014

1708

2880

2201

11320

9615

11576

10837

2H77833-2timesG73-107

2066

2101

2122

2096

5002

4053

4601

4552

7882

7430

6963

7425

3146

2565

2808

2840

12884

11482

11564

11977

3H77833-2times77245

1764

2048

2083

1965

3769

4497

4559

4275

8681

8515

8475

8557

2284

3356

3377

3006

12450

13012

13034

12832

4H77833-2times

CVJ-2-5-3-1-3

2205

2383

2733

2441

3693

5655

5220

4856

11656

10287

1010

510683

2198

4488

4176

3621

15348

1594

215325

15538

5H77833-2times1305

2209

2423

2491

2374

6008

5842

6933

6261

15238

14311

13889

14479

3758

4057

5591

4469

21246

2015

320822

2074

0

6H77833-2times(77371times

BSEC

TCP

-1)

1789

1823

1992

1868

3593

4288

4903

4262

8922

6522

7429

7625

2178

3224

3070

2824

12515

10811

12333

11886

7H77833-2times96AC

-93

1739

1960

2011

1903

4557

4350

4818

4575

7916

6068

7290

7092

2411

2529

3191

2710

12474

10419

1210

811667

8H77833-2timesTo

go-II

1927

2389

2406

2241

4185

5828

6215

5409

12319

10279

11023

11207

2433

4254

4631

3773

16504

1610

717238

16616

9H77833-2times99HS-18

1941

2245

2330

2172

4227

4139

4529

4298

9211

8120

7969

8433

3154

3184

3253

3197

13438

12259

12498

1273

210

H7729-2timesG73-107

1968

2109

2158

2079

3725

3042

3488

3418

7313

5956

6174

6481

2822

2001

2357

2393

11038

8998

9662

9899

Advances in Agriculture 11

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

11H7729-2times77245

1847

2048

2200

2032

2467

3759

5466

3898

7672

7449

8921

8014

1356

2350

3796

2500

1013

911208

14387

11911

12H7729-2times

CVJ-2-5-3-1-3

2218

2585

2767

2523

3674

4372

4483

4176

10053

9467

8664

9395

2417

3312

3345

3025

1372

813839

1314

713571

13H7729-2times1305

2169

2338

2547

2352

6728

6166

6792

6562

13333

11662

13301

1276

54128

4501

5660

4763

20061

17828

20093

19327

14H7729-2times(77371times

BSEC

TCP

-1)

1925

2095

2127

2049

3530

4165

4649

4115

9638

6917

7698

8084

1681

2833

3150

2555

1316

8110

82

12347

1219

915

H7729-2times96AC

-93

2012

2070

2180

2087

4955

4447

4596

4666

8738

6882

7762

7794

3754

3558

3181

3498

13693

11329

12359

12460

16H7729-2timesTo

go-II

2138

2428

2563

2377

4949

4660

5118

4909

8549

7243

7191

7661

3586

3353

3028

3322

13498

11903

12310

12570

17H7729-2times99HS-18

1899

1933

2073

1969

4046

4527

4988

4520

8492

7895

8144

8177

2561

3378

3553

3164

12539

12422

1313

212698

18G73-107times77245

1840

2253

2437

2177

2580

4776

5915

4424

11673

10608

10459

10913

1358

3163

4286

2936

14253

15385

16374

15337

19G73-107times

CVJ-2-5-3-1-3

2324

2537

2691

2517

4958

5030

5599

5196

9524

9395

1114

610022

3241

3645

4444

3776

14483

14425

1674

515217

20G73-107times1305

2228

2433

2583

2415

6340

5794

6373

6169

12490

1015

8119

61

11536

4199

4199

5018

4472

18830

1595

218334

1770

6

21G73-107times(77371times

BSEC

TCP

-1)

1943

2128

2220

2097

3936

3980

5770

4562

11697

9858

10303

10619

1874

2369

4181

2808

15633

13839

16073

1518

222

G73-107times96AC

-93

2118

2218

2317

2217

4270

5843

6201

5438

10319

9872

1015

910117

2703

4460

4379

3847

14589

15715

16359

15555

23G73-107timesTo

go-II

2237

2365

2733

2445

5736

5175

6822

5911

12098

10808

1299

611967

3984

3670

5372

4342

17835

1598

219818

17878

24G73-107times99HS-18

2025

2081

2322

2143

3558

4229

6612

4800

12350

9685

11516

1118

42196

2782

5009

3329

1590

813914

1812

915983

2577245timesCV

J-2-5-3-1-3

2109

2360

2675

2381

6760

6558

7299

6872

15225

11861

1416

21374

94225

4752

5839

4939

2198

518418

21461

20621

2677245times1305

1919

2100

2197

2072

5700

5142

5347

5397

11687

10565

10737

10996

4318

3956

4244

4173

17386

1570

816085

16393

2777245times(77371times

BSEC

TCP

-1)

1851

1935

2020

1936

2577

2460

2838

2625

6989

4778

4636

5468

1631

1662

1999

1764

9566

7238

7474

8093

2877245times96AC

-93

1729

1780

2133

1881

5541

5058

5748

5449

11952

8174

9526

9884

3646

3587

4071

3768

17493

13232

15274

15333

2977245timesTo

go-II

2300

2508

2658

2489

3067

5431

5963

4820

11737

11021

1216

411641

1598

4053

4770

3474

14805

16452

1812

616461

3077245times99HS-18

1982

2019

2223

2075

3846

3119

4225

3730

7493

6720

6476

6896

3205

2578

2643

2809

11339

9839

1070

110626

12 Advances in Agriculture

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

31CV

J-2-5-3-1-3times1305

2406

2553

3067

2675

4178

4796

6281

5085

13822

11425

12252

12500

2472

3241

4907

3540

18000

16222

18532

17585

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

2142

2173

2238

2184

4522

5235

5640

5132

11673

9658

10248

10526

2614

3766

4178

3519

1619

514893

15888

15659

33CV

J-2-5-3-1-3times

96AC

-93

2350

2477

2600

2476

4676

4280

6090

5015

11975

9832

10868

10891

3097

2931

4445

3491

16651

14111

1695

815907

34CV

J-2-5-3-1-3times

Togo-II

2093

2666

2989

2583

4058

3727

5190

4325

12588

10717

11217

11507

2742

2570

4185

3166

16645

14443

16407

15832

35CV

J-2-5-3-1-3times

99HS-18

2028

2311

2386

2242

3287

2513

5356

3719

10055

9733

1036

410051

2004

1581

4251

2612

13342

12246

1572

01376

9

361305times(77371times

BSEC

TCP

-1)

2005

2173

2210

2129

3569

4692

5140

4467

11652

9826

10041

10507

2005

3665

4179

3283

15221

14518

1518

214974

371305times96AC

-93

1822

2003

2047

1957

5115

4023

4813

4650

9965

7675

7571

8404

3456

2647

3155

3086

15080

11698

12384

13054

381305timesTo

go-II

2545

2583

2644

2591

6885

7283

6795

6987

12265

11410

12583

12086

4500

6120

5350

5323

1915

018693

19378

19074

391305times99HS-18

2070

2408

2593

2357

5219

4332

4800

4784

1092

97677

8968

9192

3924

3332

3840

3699

1614

912009

1376

913976

40(77371times

BSEC

TCP

-1)

times96AC

-93

2056

2115

2157

2109

3158

4463

4717

4113

9166

7540

8100

8268

1880

3542

3347

2923

12324

12003

12817

12381

41(77371times

BSEC

TCP

-1)

timesTo

go-II

1868

2253

2450

2190

4422

6458

7038

5973

1415

312369

1379

713439

2291

4646

5586

4174

18575

18827

20835

19412

42(77371times

BSEC

TCP

-1)

times99HS-18

1995

2026

2209

2077

2720

2909

3543

3057

7392

4692

5023

5702

1766

2187

2147

2033

10112

7601

8566

8760

4396AC

-93timesTo

go-II

1953

2027

2511

2164

5972

4391

7126

5830

15537

1392

714432

14632

3780

2538

5655

3991

21510

18318

21558

20462

4496AC

-93times99HS-18

1952

2021

2242

2072

3465

2758

4496

3573

5959

5186

6094

5746

2707

2154

2409

2423

9424

7944

10590

9319

45To

go-IItimes

99HS-18

1932

2008

2658

2199

5381

4995

5436

5270

10299

8876

9244

9473

3986

3842

3668

3832

15680

13871

14680

1474

3Mean

1982

2146

2301

2143

3963

4123

4836

4307

9498

8087

8663

8749

2510

2914

3513

2979

13460

12210

13499

13056

SE243

198

245

mdash671

501

626

mdash959

750

822

mdash423

349

344

mdash1155

927

1167

mdashCD

087

071

087

mdash239

178

223

mdash342

267

293

mdash15

112

412

2mdash

411

330

416

mdash

Advances in Agriculture 13Ta

ble4Analysis

ofvaria

ncefor

10times10

half-diallelfor

different

charactersin

threee

nviro

nments

(a)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Germination

()

Rateof

emergence

STI()

SSTI

()

Num

bero

fleavesseedling

Seedlin

gheight

(cm)

Freshweight

(g)seedlin

gDry

weight

(g)seedlin

g

E 1

Replicates

2652731

596768

783393

20700

93018674

33764

1033999

002590

Treatm

ents

541097

0255lowastlowast

8181153lowastlowast

30863092lowastlowast

20680900lowastlowast

1100708lowastlowast

2106317lowastlowast

930829527lowastlowast

319767lowastlowast

Parents

95118

163lowastlowast

14289925lowastlowast

59114816lowastlowast

18880254lowastlowast

1084227lowastlowast

1087906lowastlowast

22561719lowastlowast

042162lowastlowast

Hybrid

s44

11423573lowastlowast

3398933lowastlowast

25697199lowastlowast

20633716lowastlowast

472874lowastlowast

1391526lowastlowast

674123518lowastlowast

261839lowastlowast

Parentsv

ersush

ybrid

s1

43693104lowastlowast

163619

916lowastlowast

3896838

3896

2793lowastlowast

28873741lowastlowast

4272

2798lowastlowast

234144289lowastlowast

5367022lowastlowast

Error

108

281184

1356832

1173684

674337

040104

306

462

2015

1619

005535

E 2

Replicates

2583819

3707389lowast

1596635

198738

034925

448213

899529

007177

Treatm

ents

549997443lowastlowast

2825177lowastlowast

35378177lowastlowast

19074306lowastlowast

665284lowastlowast

1764

081lowastlowast

257694570lowastlowast

2177519lowastlowast

Parents

96507784lowastlowast

3718

879lowastlowast

69866289lowastlowast

1798

5404lowastlowast

705379lowastlowast

1408518lowastlowast

10612519lowastlowast

077429lowastlowast

Hybrid

s44

10569731lowastlowast

2127302lowastlowast

2910

2537lowastlowast

19429240lowastlowast

329256lowastlowast

7000

03lowastlowast

186026552lowastlowast

1483428lowastlowast

Parentsv

ersush

ybrid

s1

16223710lowastlowast

25488361lowastlowast

1113

326

13257362lowastlowast

15089673lowastlowast

5178

3569lowastlowast

5634825791lowastlowast

51618348lowastlowast

Error

108

334760

1097039

1049505

635570

06764

116

0576

694613

006206

E 3

Replicates

2894398

4808815lowast

mdashmdash

051076

095989

3768421

030220

Treatm

ents

541261560

0lowastlowast

4842216lowastlowast

mdashmdash

2811077lowastlowast

5947316lowastlowast

3870844

50lowastlowast

3445076lowastlowast

Parents

97135

735lowastlowast

1097

6388lowastlowast

mdashmdash

1468699lowastlowast

1112

764lowastlowast

136081650lowastlowast

1307836lowastlowast

Hybrid

s44

13217740lowastlowast

1379360

mdashmdash

15540

71lowastlowast

4581632lowastlowast

252084767lowastlowast

2378032lowastlowast

Parentsv

ersush

ybrid

s1

35440

180lowastlowast

102000335lowastlowast

mdashmdash

70200767lowastlowast

109548362lowastlowast

8586095705lowastlowast

69630188lowastlowast

Error

108

782907

1085444

mdashmdash

139771

291325

3019

169

02400

4(b)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Panicle

emergence

Effectiv

etillersplant

Planth

eight

Earlength

Earw

eightp

lant

Dry

fodd

eryieldplant

Grain

yieldplant

Totalbiological

yieldplant

E 1

Replicates

231860

61lowastlowast

416348lowastlowast

1672

9472lowast

1706

411077192

9634539

392777

115544

59Treatm

ents

544152

368lowastlowast

085657lowastlowast

326706089lowastlowast

1602572lowastlowast

6177

0406lowastlowast

304113

204lowastlowast

34285030lowastlowast

575617

582lowastlowast

Parents

94511481lowastlowast

018538lowastlowast

140807476lowastlowast

2240093lowastlowast

11653

959lowastlowast

27014155lowastlowast

3858650lowastlowast

61321919lowastlowast

Hybrid

s44

3201347lowastlowast

100797lowastlowast

110395657lowastlowast

1010

497lowastlowast

40296671lowastlowast

171271411lowastlowast

2413

6880lowastlowast

302999650lowastlowast

Parentsv

ersush

ybrid

s1

4276

5286lowastlowast

02360

9lowast11517452

616lowastlowast

2191

6167lowastlowast

1457662749lowastlowast

8643043524lowastlowast

7546

41053lowastlowast

17199467546lowastlowast

Error

108

468159

004907

3692099

224610

17114

303498126

682350

5075268

E 2

Replicates

2656364

002556

61575565lowastlowast

828842lowastlowast

589094

2268506

260877

1507116

Treatm

ents

542999618lowastlowast

069822lowastlowast

268998723lowastlowast

2436886lowastlowast

60592784lowastlowast

247259877lowastlowast

4192

0073lowastlowast

513483489lowastlowast

Parents

926300

00lowastlowast

048079lowastlowast

165555

405lowastlowast

3871874lowastlowast

1412

2393lowastlowast

19543682lowastlowast

3132

426lowastlowast

57230706lowastlowast

Hybrid

s44

2533

165lowastlowast

07344

9lowastlowast

9616

2857lowastlowast

1463359lowastlowast

35495366lowastlowast

154759288lowastlowast

26262465lowastlowast

293492304lowastlowast

Parentsv

ersush

ybrid

s1

26850135lowastlowast

105929lowastlowast

880476

6697lowastlowast

32357161lowastlowast

1583112746lowastlowast

6366731560lowastlowast

107994

3646lowastlowast

14299370699lowastlowast

Error

108

507598

005829

5234122

149406

955133

2142411

462311

3273886

E 3

Replicates

2029697

021161

9459847

143746

985141

46800

6492

6334

3345160

Treatm

ents

548045499lowastlowast

094511lowastlowast

192420108lowastlowast

3279225lowastlowast

72049211lowastlowast

298953

021lowastlowast

55061308lowastlowast

651732994lowastlowast

Parents

98010

741lowastlowast

094166lowastlowast

116037343lowastlowast

3702185lowastlowast

16305190lowastlowast

2323644

0lowastlowast

4553

893lowastlowast

71484982lowastlowast

Hybrid

s44

6254377lowastlowast

095514lowastlowast

7378

1513lowastlowast

2188348lowastlowast

31813193lowastlowast

190263916lowastlowast

31572179lowastlowast

368689934lowastlowast

Parentsv

ersush

ybrid

s1

8716

7710lowastlowast

053496lowast

609996

3199lowastlowast

47471212lowastlowast

2344

130203lowastlowast

756272

2861lowastlowast

154314

9707lowastlowast

18327859

719lowastlowast

Error

108

696981

007883

5578505

228608

1491014

2574

223

449588

5187705

lowast

Sign

ificant

at119875=005

lowastlowastSign

ificant

at119875=001

14 Advances in Agriculture

Table 5 Mean performance of the genotypes for membrane thermostability in two environments

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594

Advances in Agriculture 15

Table 5 Continued

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434

Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash

lowastValues are transformed values

0

6

12

18

24

30

36

42

48

54

minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity

G73-10796AC-93CVJ-2-5-3-1-3

Togo-II99HS-18

H77833-2H7729-2772451305

H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305

1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305

H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305

77245 times 1305

77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305

77371 times BSECT CP-1

H77833-2 times G73-107

Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits

moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling

survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The

16 Advances in Agriculture

Table6Po

oled

correla

tioncoeffi

cientamon

gvario

uscharactersin

threee

nviro

nments

Characters

Germination

Rateof

emergence

STI

SSTI

Leaves

seedlin

gSeedlin

gheight

Fresh

weight

Dry

weight

MTS

Panicle

emergence

Effectiv

etillersplant

Plant

height

Earlength

Ear

weightp

lant

Dry

fodd

eryield

plant

Grain

yield

plant

Total

biological

yield

plant

Germination

1000

0357lowastlowast

0353lowastlowast

0590lowastlowast

0230

0373lowastlowast

0293lowast

0260

0276lowastminus0135minus0478lowastlowast

0326lowast

minus0069minus0104

minus0190

minus0145

minus0167

Rateof

emergence

1000

0233

0351lowastlowast

0605lowastlowast

0585lowastlowast

0551lowastlowast

0524lowastlowast

0139minus0593lowastlowast

0032

0517lowastlowast

0217

0388lowastlowast

0361lowastlowast

0348lowastlowast

0377lowastlowast

STI

1000

0837lowastlowast

0241

0129

0081

004

80890lowastlowast

minus0215

minus0211

0030minus0007minus0043

minus0101

minus0111

minus0084

SSTI

1000

0233

0264

0183

0140

0745lowastlowast

minus0247minus0268lowast

0184minus0017minus006

6minus0129

minus0144

minus0112

Num

bero

fleaves

1000

0683lowastlowast

0633lowastlowast

060

4lowastlowast

0176minus0474lowastlowast

0109

0576lowastlowast

0496lowastlowast

064

1lowastlowast

0607lowastlowast

0630lowastlowast

0630lowastlowast

Seedlin

gheight

1000

0703lowastlowast

0663lowastlowast

0031minus0493lowastlowast

minus0115

0735lowastlowast

0360lowastlowast

0526lowastlowast

0520lowastlowast

0515lowastlowast

0532lowastlowast

Freshweight

1000

0957lowastlowast

minus0016

0425lowastlowast

minus0082

0661lowastlowast

0351lowastlowast

0581lowastlowast

0496lowastlowast

0576lowastlowast

0533lowastlowast

Dry

weight

1000minus0033minus0428lowastlowast

minus0016

0634lowastlowast

0354lowastlowast

0620lowastlowast

0509lowastlowast

0614lowastlowast

0554lowastlowast

MTS

1000minus040

minus0177minus0037

0015

minus0074

minus0138

minus0131

minus0120

Panicle

emergence

1000

minus0150minus0269lowast

0022minus0329lowast

minus0337lowast

minus0280lowast

minus0341lowast

Effectiv

etillersplant

1000

minus0012

0170

0366lowastlowast

040

5lowastlowast

0323lowast

0401lowastlowast

Planth

eight

1000

0650lowastlowast

0603lowastlowast

0596lowastlowast

0619lowastlowast

0610lowastlowast

Earlength

1000

0608lowastlowast

0671lowastlowast

064

8lowastlowast

066

4lowastlowast

Earw

eightp

lant

1000

0910lowastlowast

0984lowastlowast

0957lowastlowast

Dry

fodd

eryield

plant

1000

0907lowastlowast

0991lowastlowast

Grain

yield

plant

1000

0950lowastlowast

Totalbiological

yield

plant

1000

lowastSign

ificant

at119875=005lowastlowastSign

ificant

at119875=001

Advances in Agriculture 17

faster germination and early vigour in seedling could act asphysical indicators for heat tolerance

Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits

Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics

Conflict of Interests

The authors declare that there is no conflict of interestsregarding the publication of this paper

References

[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981

[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993

[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006

[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993

[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013

[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007

[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965

[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011

[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001

[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009

[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962

[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967

[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963

[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001

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Page 2: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

2 Advances in Agriculture

Table 1 Description of pearl millet genotypes used as parents for hybridization

Sr No Genotype Response Status Origin and characteristics

1 H77833-2 Tolerant Inbred Bred at CCSHAU Hisar by selfing and selection within a Rajasthanilandrace population

2 H7729-2 Susceptible InbredBred at CCSHAU Hisar It is medium dwarf nodalpigmentationmdashviolet brown resistant to downy mildew shoot flygrey weevil chaffer beetle and leaf roller

3 G73-107 Tolerant Inbred Bred at CCSHAU Hisar and has profuse tillering narrow leaves andtypical orange brown anthers

4 77245 Susceptible Inbred Bred at CCSHAU Hisar by selfing and selection It is late floweringand high tillering

5 CVJ-2-5-3-1-3 Tolerant Inbred Dwarf developed at ICRISAT Patancheru

6 1305 Susceptible Inbred Bred at CCSHAU Hisar late flowering medium dwarf semicompactearhead

7 (77371 times BSECT CP-1) Tolerant Inbred Bred at CCSHAU Hisar using BSECT CP-1 from ICRISATPatancheru as male parent

8 96AC-93 Tolerant Inbred Resistant to downy mildew bred at CCSHAU Hisar by selfing andselection

9 Togo-II Tolerant Inbred Selection from ICRISAT material-Togo bred at CCSHAU Hisar10 99HS-18 Tolerant Inbred Bred at CCS Haryana Agricultural University Hisar

Table 2 Details of different environments created through sowing dates

Environment Nature of environment Date of sowing Site Location

Environment-1 (E1) Heat stress May 21 Research area Department ofGenetics and Plant Breeding

CCS HAU Hisar Haryana

Environment-2 (E2) Heat stress June 13 Research area Department ofGenetics and Plant Breeding

CCS HAU Hisar Haryana

Environment-3 (E3)(during monsoon season)

Nonstress July 17 Research area Department ofGenetics and Plant Breeding

CCS HAU Hisar Haryana

germination and early seedling stages in pearl millet and toidentify some promising F

1hybrids improvement for this

trait particularly for commercial cultivation

2 Materials and Methods

21 Plant Material A field screening technique that isthermotolerance index [4] was applied to screen the pearlmillet F

1hybrids for heat tolerance at seedling stage This

technique could be used in the hottest months (May-June)only with no rainfall Since the technique was applicableto the very young seedlings of pearl millet it was also ofconcern to test the association of seedling thermotoleranceto other phenotypic characteristics of seedlings The mem-brane thermostability (based on quantification of electrolyteleakage) was also conducted for screening the pearl milletF1hybrids at seedling stage Ten pearl millet genotypes

namely H77833-2 H7729-2 G73-107 77245 CVJ-2-5-3-1-3 1305 77371 times BSECT CP-1 96AC-93 Togo-II and 99HS-18 were selected from the germplasm on the basis of differentresponse to supraoptimal temperature tolerance (Table 1) inseparate experiment [5] These were crossed in a half-diallelmating system (excluding reciprocals) The 45 F

1hybrids

produced were tested along with parents for heat toleranceand related traits at seedling stage

22 Experimental Site and Conditions of Growth The exper-iment was conducted at the research area of Departmentof Genetics and Plant Breeding Chaudhary Charan SinghHaryana Agricultural University Hisar (Lat 29∘ 101015840N Long75∘ 461015840E and 2152m above mean sea level) located insubtropical region of Haryana India The 45 F

1hybrids

produced were tested along with parents for heat toleranceand related traits in three replicated plots under supraop-timal temperature exposure at seedling stage in two stressenvironments (environment-1 and environment-2) createdthrough different dates of sowing on May 21 and June 13 (thehottest period in North India) respectively so as to conductthe experiment under different range of temperatures withno rainfall A presowing medium light irrigation was donethrough flooding and the field was precisely levelled beforesowing After sowing no further irrigation was given to thestressed environments (environment-1 and environment-2)One nonstress environment (Table 2) was created throughsowing on July 17 (normal sowing period during monsoon)Each genotype was grown in 3 rows each of 3m length spaced

Advances in Agriculture 3

0

10

20

30

40

50

60

70

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15DAS

E1

E2

E3

Tem

pera

ture

(∘C)

(a)

05

101520253035404550

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15DAS

E1

E2

E3

Tem

pera

ture

(∘C)

(b)

0

10

20

30

40

50

60

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16DAS

E1

E2

E3

Tem

pera

ture

(∘C)

(c)

02468

101214161820

1 3 5 7 9 11 13 15

Moi

sture

()

DAS

E1

E2

E3

Permanent wilting point

(d)

Figure 1 (a) Soil surface temperature (b) air temperature (c) temperature 5 cm above the soil surface and (d) soil moisture status in differentenvironments

30 cm apart with 10 cm plant to plant spacingThe seeds weresown manually

The soil of experimental field was sandy loam in textureThe water holding capacity field capacity and permanentwilting point of the experimental field were 40 17 and 6percent respectively [6] Irrigation was withheld during thecourse of experiment however drought stress-free condi-tions were ensured through monitoring at regular interval(on alternate days) by measuring the moisture status of thesoil by gravimetric method The soil samples of about 50 gwere taken up to 20 cmdepth from the experimental plotThemoisture content on dry weight basis may be calculated [7]using the following formula

Soil moisture ()

=

(Weight of wet soil minusWeight of dry soil)Weight of dry soil

times 100

(1)

The heat stress period was terminated by irrigating theexperimental field before reaching the soil moisture level atpermanent wilting point A condition of no drought wasmaintained in order to determine the exclusive effect of tem-perature at seedling stageThis was maintained by measuringthe moisture status of the soil by gravimetric method onalternate days The soil surface temperature was measuredby a soil thermometer at soil surface temperature at 5 cmabove the soil surface and air temperature at 1m above thesoil surface were recorded between 200 pm and 230 pmdaily (Figure 1)

Emphasis of observation is centred on germination sur-vival and mortality of the seedlings

23 Plant CharacteristicsScreening Techniques The empha-sis was given to the observations of germination survival andmortality of the seedlingsThe seedlings were inspected everyday in the morning The seedlings which had died due to

4 Advances in Agriculture

heat stress were noted in each genotype on daily basis Obser-vations continued till the surviving seedlingswere established(15 DAE) and there was no further mortality Moreover thesoil moisture was depleting near to the permanent wiltingpoint and drought stress could also come into play along withheat stress to further continue the experiment Since experi-ment proceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedlingsurvival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of heat stress at seedling stage was observed on latergrowth stages

231 Thermotolerance Characteristics For heat tolerancecharacteristics 3 indices namely seedling thermotoleranceindex (STI) [4] seed to seedling thermotolerance index(SSTI) [8] and membrane thermostability (MTS) wereapplied to evaluate the hybrids and parents STI was cal-culated as the ratio of number of seedlings survived to thenumber of seedling emergences expressed in percentageSeed to seedling thermotolerance index (SSTI) was calculatedas the ratio of seedling survival to the number of seedlingsexpected to emerge expressed in percentage [8] The SSTIis an extension of STI by taking expected germination intoaccount It was necessary to correct the effect of under soilmortality (USM) The loss of germination due to heat (ieunder soil mortality) can be measured only after a standardgermination test Therefore the germination of seed fromthe same lot under monsoon environment (main pearl milletgrowing season where no under soil mortality occurreddue to heat stress) was taken as expected germination forcalculation of SSTI

232 Membrane Thermostability These genotypes were alsoscreened through laboratory technique (membrane ther-mostability) [9 10] The young leaves of 21-day-old seedlingsof each genotype in three replication plots were used formembrane thermostability test A middle portion of 10 cmfrom each leaf was taken and the midrib was removed andtwo equal halves of the 10 cm each were made Each halfwas taken to provide replications Each leaf portion was cutinto pieces and thoroughly washed and rinsed with distilledwater two times to remove the electrolytes adhering to theplant tissue as well as electrolyte released from the cutting ofplant tissue and put them into test tubes After final rinsingtubes were drained maintaining sufficient water to preventdesiccation of plant material during heat treatment Thesetest tubes were covered with aluminium foil and incubated inwater bath at 55∘C for 15 minutes for giving heat treatmentAfter the heat treatment period 10mL of distilled waterwas added to the test tubes Then all the test tubes wereheld at 10∘C overnight to allow diffusion of electrolytes fromplant material Test tubes were brought out of refrigeratorand shaken well to mix the contents An initial electricalconductivity (EC) of both replications of each treatment (119879

1)

was determined with electrical conductivity meter Thenthese test tubes were autoclaved at 15 psi for 2 minutes forcomplete electrolyte leakage from the leaf samples These testtubes were then cooled at room temperature and contentsmixed and final EC was taken for all the treatments (119879

2)

Membrane thermostability (MTS) was calculated accordingto Ibrahim and Quick [9] and Yadav et al [10] as follows

Membrane Thermostability (MTS) = (1 minus1198791

1198792

) times 100

(2)

where 1198791is conductivity reading after heat treatment and 119879

2

is conductivity reading after autoclavingCompletely randomized design (CRD) for membrane

thermostabilitywas conducted to detect variations among thegenotypes

233 Morphological Characteristics The seedling growthcharacteristics namely germination () emergence rate(ER) number of leavesseedling (two-week stage) seedlingheight (two-week stage) seedling fresh weight (g) (four-week stage) and seedling dry weight (g) (four-week stage)were recorded The data for seedling height and number ofleaves per seedling was recorded on 10 random seedlings perplot Emergence rate (an index for speed of germination)can be used as a tool in pearl millet breeding programs forevaluation of seedling vigour Speed of germination is oneof the oldest concepts of seedling vigour The emergencerate (ER) was calculated by dividing the number of normalseedlings obtained at each counting by the number of daysseeds had been in the seed bed (DAS)The values obtained ateach count were then summed at the end of the germinationtest to obtain the emergence rate [11]

Emergence Rate (ER) =Seedling count

Days to first count+ sdot sdot sdot

+

Seedling countDays to final count

(3)

After termination of the heat stress on the seedlings byirrigating the field the experiment was continued to studythe effect of seedling heat stress on maturity traits on thesurvived plants The maturity traits observed were panicleemergence effective tillersplant plant height ear length earweightplant dry fodder yieldplant grain yieldplant andtotal biological yieldplant

24 Statistical Analysis Analysis of variance for randomizedblock design (RBD) for field traits that is germinationemergence rate number of leavesseedling seedling heightseedling fresh weight and seedling dry weight and com-pletely randomized design (CRD) for membrane thermosta-bility test was conducted under each environment on meanbasis [12] Analyses were done using the software OPSTATdeveloped at CCS HAU Hisar Correlations among the vari-ous characters studied were calculated to establish the natureand magnitude of associations among the traits [8 13] with

Advances in Agriculture 5

the software SPSS (SPSS Inc) Multivariate clustering wasdone based on paired group cluster method using similaritycoefficient under Past 140 software [14] Multivariate analysisbased on the Euclidean distancematrix was estimated amonggenotypes to distinguish genotypes from each other Thegrouping of genotypes was generated using paired groupcluster method

3 Results and Discussion

The excessive heat (soil temperature up to 630∘C in E2)

about 18∘C higher than the normal atmospheric temperature(Figures 1(a) and 1(b)) was responsible for mortality ofseedlings Many seedlings were found desiccated at the pointof contact besides the desiccation at the tipsThe temperaturearound the tip level (5 cm above the soil surface) also reachedup to 50∘C (Figure 1(c)) It could therefore be concluded thatthe seedlings had to survive in a really high temperature Themoisture level remained above the permanent wilting point(Figure 1(d)) therefore drought effect was not in the playThe heat tolerance was measured as STI (Table 3) throughthe field screening technique of Peacock et al [4] Anotherindex known as SSTI was also calculated as an improvementand extensionThe analysis of variance (RBD) for 8 charactersin 3 environments showed significant genotypic differencesNo variability was observed for STI and SSTI in E

3only In

this environment no seedling mortality was observed andthe germination was taken as standard The values for STIand SSTI were therefore equal and the highest for all thegenotypes in this environment and hence no variation wasobserved

The hybrids were evaluated on the basis of mean perfor-mance for various characters in each environment (Table 3)On the mean basis STI value ranged from 5332 to 8224with an average of 7072 The hybrid H7729-2 times CVJ-2-5-3-1-3 was the best scorer for heat tolerance followed byH77833-2 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II The hybrid H77833-2 times 1305 was found susceptible toheat stress Seed to seedling thermotolerance index (SSTI)which is expected to be more rigorous index deviated thevalues from STI according to variation in germination undernormal environment (E

3) as the SSTI also coversincludes

the under surface mortality (USM) during germinationThe reductions in values were not strictly proportional tothe STI The reduction is dependent on thermosensitivityof germination We also came across such hybrids whichshowed poor germination and high STI SSTI values rangedfrom 4605 to 7287 with an average of 6090 The genotypeH77833-2 times 96AC-93 had the highest worth for SSTI onmean basis followed byG73-107times (77371timesBSECTCP-1) andH77833-2 times (77371 times BSECT CP-1) (Table 3) The analysisof variance for ten different characters in three differentenvironments (two stressed and one normal) was carried outand the results showed significant genotypic differences forall the characters (Table 4)

A caution is needed for evaluating such genotypes underSTI The SSTI gave a highly reduced value putting thesecrosses in the susceptible list It may be concluded therefore

that SSTI is more dependable and desirable tool for screeningand evaluating the genotypes for heat tolerance The germi-nation was lower in E

2than in E

1 This period has the highest

range of temperature with a high average Genotype-wise thehighest germination (6209) was recorded for the genotypeH7729-2 times (77371 times BSECT CP-1) on mean basis whichgives a preliminary indication of resistance to heat while thegenotype 1times 6 had the lowest germination (3533)The hybridH77833-2 times G73-107 was adjudged as the fastest emergenceof seedlingsThe genotype (77371timesBSECTCP-1)times 96AC-93had the highest fresh weightseedlingThe genotype H7729-2 times Togo-II had the highest seedling dry weight E

1had lower

dry weightseedling than E2indicating the more heat stress

during the E1

31 Membrane Thermostability Membrane thermostability(MTS) has long been recognised as a parameter of heattolerance All the hybrids were put to membrane thermosta-bility test in the laboratory under standard protocol Acompletely randomised design (CRD) analysis was carriedout for membrane thermostability (MTS) The mean sum ofsquares due to genotypes was highly significant indicatingthat enough genetic variability was present for membranethermostability (MTS) in each environment which justifiedfurther analysis of the data The range of values lay between5073 and 7742 Among the parents H77833-2 G73-107CVJ-2-5-3-1-3 77371 times BSECT CP-1 96AC-93 Togo-II and99HS-18 were verified as resistant genotypes and H7729-2 77245 and 1305 genotypes were found susceptible asmentioned in Table 1 (on the basis of previous experiment)Among all the hybrids H7729-2 times CVJ-2-5-3-1-3 CVJ-2-5-3-1-3 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II werethree highly tolerant genotypes on the basis of membranethermostability (Table 5)

32 Correlations The pooled correlations among the charac-ters were calculated (Table 6) around heat tolerance indices(STI SSTI and membrane thermostability) with a view tofind whether there was any association of these characterswith seedling as well as maturity traits It was observedthat STI and SSTI recorded the highest significant cor-relation among themselves as they are related indices forheat tolerance Membrane thermostability also showed highsignificant correlation with STI as well as SSTI STI hada significant positive association with germination of seedonly among seedling and maturity traits studied SSTI inaddition was also positively correlated with germination andemergence rate and negatively associated with number ofeffective tillersplantMembrane thermostability also showedsignificant positive correlation with seed germination amongseedling and maturity traits studied While evaluating thediallel progenies it was also seen that the heat toleranceindices STI SSTI and membrane thermostability were notshowing any perceptible correlation with the rest of thedevelopmental traits (seedling andmaturity traits)The stressperiod was terminated by irrigating the field experiment after15 days fromdate of sowingwhen the seedlings get establishedand further mortality stopped due to heat Moreover the soil

6 Advances in Agriculture

Table3Perfo

rmance

ofthep

arentsandthec

rosses

ford

ifferentcharacters

(a)

Sr

No

Genotypes

Germination(

)Ra

teof

emergencelowast

STIlowast

()

SSTIlowast(

)Num

bero

fleavesseedlin

gE 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

3941

3737

6164

4614

4037

4109

4279

4142

7830

7387

9000

8072

4498

4133

9000

5877

661

502

800

654

2H7729-2

5513

4726

6092

5443

2815

4980

3258

3684

4835

4495

9000

6110

4441

3602

9000

5681

363

294

427

361

3G73-107

5037

4826

5918

5260

4534

4132

4481

4382

6187

5413

9000

6866

5213

4460

9000

6224

878

710

935

841

477245

4747

4624

5565

4978

3625

4604

4047

4092

4385

3580

9000

5655

3803

3040

9000

5281

377

292

437

369

5CV

J-2-5-3-1-3

4824

4832

6897

5518

5044

4907

4960

4970

7193

6813

9000

7669

4959

4785

9000

6248

802

580

923

768

61305

4326

3891

5404

4540

3376

5056

3573

4002

3903

3281

9000

5395

3211

2452

9000

4888

350

284

414

349

777371timesBS

ECTCP

-14747

4502

6320

5190

4934

5075

5065

5025

6952

6706

9000

7552

5056

4671

9000

6242

621

542

700

621

896AC

-93

4651

3818

5722

4730

4312

4915

4753

4660

6660

6001

9000

7220

5212

3920

9000

6044

701

590

899

730

9To

go-II

4613

3937

5276

4609

4245

4737

4797

4593

7198

6861

9000

7686

5980

4767

9000

6582

681

629

853

721

1099HS-18

4575

3823

6247

4882

4317

4659

4746

4574

7594

7319

9000

7971

5154

4172

9000

6109

745

554

915

738

1H77833-2timesH7729-2

5787

5295

6733

5938

4964

5015

4989

4989

6803

6504

9000

7436

5808

5134

9000

6647

711

585

780

692

2H77833-2timesG73-107

5472

4417

6405

5431

5448

5422

5239

5370

7231

6769

9000

7667

5980

4556

9000

6512

1101

705

1340

1049

3H77833-2times77245

4076

3707

5504

4429

5217

5446

5238

5300

6627

6224

9000

7284

4690

4037

9000

5909

740

525

965

744

4H77833-2timesCV

J-2-5-3-1-3

4843

4300

5856

5000

4862

4956

4913

4911

7061

6519

9000

7527

5575

4632

9000

6403

998

885

1258

1047

5H77833-2times1305

3296

2780

4521

3533

5076

4907

5238

5074

3732

3265

9000

5332

2765

2050

9000

4605

1192

934

1600

1242

6H77833-2times(77371times

BSEC

TCP

-1)5636

5539

6258

5811

4489

5219

5015

4908

6981

6482

9000

7488

6146

5680

9000

6942

898

726

1525

1050

7H77833-2times96AC

-93

5720

5515

6335

5857

4800

5186

5075

5021

7833

7786

9000

8206

6603

6256

9000

7287

1107

873

1193

1058

8H77833-2timesTo

go-II

4862

3453

5709

4675

5036

4829

4911

4925

6190

5500

9000

6897

5214

3339

9000

5851

979

670

1107

919

9H77833-2times99HS-18

4575

4418

5447

4813

5041

5074

5006

5040

5800

5073

9000

6625

4846

4133

9000

5993

978

733

1135

949

10H7729-2timesG73-107

5615

4962

6273

5617

5023

5535

5165

5241

6143

5501

9000

6881

5540

4441

9000

6327

1000

720

1363

1028

11H7729-2times77245

5472

5164

6281

5639

4745

5031

5028

4935

5572

5175

9000

6582

4901

4345

9000

6082

880

620

897

799

12H7729-2timesCV

J-2-5-3-1-3

5494

5124

6536

5718

4804

5316

4957

5026

7596

8076

9000

8224

6093

5680

9000

6924

981

932

1451

1121

13H7729-2times1305

3804

3826

5511

4380

3596

4724

4387

4236

6362

7075

9000

7479

4230

4517

9000

5916

926

725

1497

1049

14H7729-2times(77371times

BSEC

TCP

-1)

6369

4847

7412

6209

4847

5351

5246

5148

6377

6031

9000

7136

5683

4230

9000

6304

977

671

1380

1009

15H7729-2times96AC

-93

5314

4657

6702

5558

4514

5295

5102

4971

5612

4856

9000

6489

4613

3603

9000

5739

846

679

1397

974

16H7729-2timesTo

go-II

5827

5594

6651

6024

5072

5279

5271

5207

6563

5371

9000

6978

5763

4652

9000

6472

873

719

1040

877

17H7729-2times99HS-18

5741

4638

7918

6099

4847

5024

5021

4964

6179

5603

9000

6927

4882

3725

9000

5869

1012

730

1600

1114

18G73-107times77245

5115

4646

6103

5288

4977

5242

5450

5223

7006

6063

9000

7356

5685

4594

9000

6426

1021

790

1292

1034

19G73-107timesCV

J-2-5-3-1-3

5134

4894

6427

5485

4901

5317

5181

5133

6637

6124

9000

7254

5253

4710

9000

6321

1039

863

1254

1052

20G73-107times1305

4881

4561

7597

5680

4717

4791

4775

4761

6704

6380

9000

7361

4556

4115

9000

5890

1140

882

1657

1226

21G73-107times(77371times

BSEC

TCP

-1)

5636

5519

6283

5812

4986

5219

5203

5136

7990

6203

9000

7731

6620

5436

9000

7019

977

703

1395

1025

22G73-107times96AC

-93

5740

4510

6415

5555

5129

4982

5195

5102

6978

5556

9000

7178

6156

4019

9000

6392

1077

860

1264

1067

23G73-107timesTo

go-II

4460

4357

6044

4953

4396

5138

4951

4829

5279

4835

9000

6371

4018

3603

9000

5540

906

881

1189

992

24G73-107times99HS-18

5473

4356

6374

5401

5102

4955

5055

5037

6460

5197

9000

6886

5534

3704

9000

6079

1171

766

1635

1191

2577245timesCV

J-2-5-3-1-3

4766

4666

5921

5117

5170

4918

4900

4996

5928

5859

9000

6929

4785

4613

9000

6133

984

920

1194

1033

2677245times1305

4575

4060

6052

4895

5128

5297

5203

5210

5102

4990

9000

6364

3979

3464

9000

5481

1279

823

1550

1217

2777245times(77371times

BSEC

TCP

-1)

5017

4929

6022

5322

5108

5473

5274

5285

5308

5044

9000

6451

4498

4191

9000

5896

833

588

939

787

Advances in Agriculture 7

(a)Con

tinued

Sr

No

Genotypes

Germination(

)Ra

teof

emergencelowast

STIlowast

()

SSTIlowast(

)Num

bero

fleavesseedlin

gE 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

2877245times96AC

-93

5472

4705

622154

66

5135

5197

5006

5113

5247

4843

9000

6363

4708

3784

9000

5831

795

662

1058

838

2977245timesTo

go-II

5252

4492

5889

5211

5188

4861

5074

5041

4885

4263

9000

6049

4421

3379

9000

5600

977

751

1243

990

3077245times99HS-18

5740

4099

6872

5570

5249

4898

5258

5135

5628

4616

9000

6415

4863

3014

9000

5626

1045

863

1184

1031

31CV

J-2-5-3-1-3times1305

4364

3944

5700

4669

4974

4517

4650

4714

6097

5579

9000

6892

4594

3842

9000

5812

936

848

1403

1063

32CV

J-2-5-3-1-3times(77371times

BSEC

TCP

-1)4345

3808

6774

4976

5272

4996

5035

5101

6650

5766

9000

7139

4288

3381

9000

5556

800

712

1013

841

33CV

J-2-5-3-1-3times96AC

-93

4862

4197

5743

4934

4884

4980

5058

4974

7477

6760

9000

7745

5957

4709

9000

6555

933

738

1478

1050

34CV

J-2-5-3-1-3timesTo

go-II

4670

4343

6233

5082

4399

4813

4631

4614

5806

6614

9000

7140

4422

4479

9000

5967

963

855

1180

999

35CV

J-2-5-3-1-3times99HS-18

5493

5080

7273

5949

5276

4984

5162

5141

7322

6590

9000

7637

5534

4766

9000

6433

998

807

1338

1047

361305times(77371times

BSEC

TCP

-1)

4613

4539

5717

4956

4986

4955

5080

5007

5137

4870

9000

6336

4210

3936

9000

5715

863

630

913

802

371305times96AC

-93

5473

5177

6830

5827

5224

4779

4919

4974

7117

6965

9000

7694

5640

5220

9000

6620

875

694

1476

1015

381305timesTo

go-II

4230

3420

5262

4304

4309

456144

45

4438

5781

4373

9000

6385

4575

2887

9000

5487

637

566

794

666

391305times99HS-18

5433

4507

6947

5629

5225

4753

4991

4989

7287

6968

9000

7752

5576

4479

9000

6352

1115

818

1410

1114

40(77371times

BSEC

TCP

-1)times

96AC

-93

5595

4429

7461

5828

5216

4955

5121

5097

7087

6368

9000

7485

5432

4038

9000

6157

955

753

1462

1057

41(77371times

BSEC

TCP

-1)times

Togo-II

5252

4728

6106

5362

5492

4808

5132

5144

8025

7505

9000

8177

6310

5413

9000

6908

859

693

990

848

42(77371times

BSEC

TCP

-1)times

99HS-18

5595

4298

6295

5396

4729

4887

5031

4883

7366

6945

9000

7770

6312

4556

9000

6623

903

622

1125

883

4396AC

-93timesTo

go-II

5017

3977

6486

5160

5019

5195

5237

5150

5922

5486

9000

6803

4689

3484

9000

5724

1007

728

1167

967

4496AC

-93times99HS-18

5595

4812

6924

5777

5036

5385

5205

5209

7411

5378

9000

7263

5873

3979

9000

6284

1013

652

1334

1000

45To

go-IItimes

99HS-18

4670

4497

7293

5487

4718

4319

4796

4611

5688

5431

9000

6706

3980

3665

9000

5548

939

653

1462

1018

Mean

5043

4482

6271

5265

4792

498149

23

4899

6377

584190

00

7072

5079

4190

9000

6090

898

701

1168

922

SE097

106

161

mdash213

191

190

mdash19

818

7mdash

mdash14

914

6mdash

mdash036

047

068

mdashCD

272

296

453

mdash597

537

534

mdash555

525

mdashmdash

421

409

mdashmdash

103

133

192

mdashlowastTransfo

rmed

data

(b)

Sr

No

Genotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

908

785

2245

1313

859

1537

1924

1440

154

143

160

152

6267

4800

3833

4967

212

227

249

229

15644

15458

13267

1479

02

H7729-2

1125

1175

1789

1363

317

2400

2811

1843

093

191

252

179

6700

5333

5100

5711

185

210

234

210

18850

18565

15389

17601

3G73-107

1166

1475

1966

1536

943

1744

4945

2544

143

129

366

213

6700

5433

5300

5811

218

219

238

225

15009

1496

513503

14492

477245

1438

1373

2085

1632

607

1863

5957

2809

117

180

509

269

6100

4800

5100

5333

232

221

253

235

14036

13802

11742

1319

35

CVJ-2-5-3-1-3

1214

1437

1807

1486

1163

1610

4166

2313

171

141

407

240

6300

4933

4600

5278

239

227

270

245

1994

317261

1497

017391

61305

858

1137

1737

1244

540

967

4861

2123

125

088

417

210

6533

5033

4800

5456

244

213

257

238

15527

15405

12274

14402

8 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

777371timesBS

ECT

CP-1

1377

1487

1892

1585

928

2660

3330

2306

150

258

301

236

5733

4533

4700

4989

242

220

293

252

1513

814550

1315

014279

896AC

-93

1303

1259

1852

1471

1056

1445

5972

2825

185

147

586

306

5500

4833

3833

4722

256

304

318

293

15577

14032

10000

13203

9To

go-II

1056

1220

2272

1516

924

1466

9113

3834

146

129

883

386

6167

4533

4600

5100

185

149

124

153

14578

12073

10033

12228

1099HS-18

1277

1075

1808

1387

1241

2833

7070

3715

228

226

615

356

6100

5000

4167

5089

243

173

324

247

12627

10355

1017

311052

1H77833-2times

H7729-2

1432

1498

2233

1721

1350

4405

8373

4709

159

499

782

480

5767

4433

3800

4667309

290

248

282

21010

18071

15847

18309

2H77833-2times

G73-107

1675

1635

2205

1838

1236

3211

5789

3412

134

313

547

331

5833

4767

3833

4811

262

295

236

265

21253

20449

15808

1917

0

3H77833-2times

77245

1577

1522

2263

1787

1058

6933

8719

5570

255

553

782

530

5267

4633

4600

4833

273

201

265

246

2074

119511

15208

18487

4H77833-2times

CVJ-2-5-3-1-3

1791

1757

2165

1904

20218030

5276

5109

354

779

477

537

5533

4233

3767

4511

165

330

351

282

21827

20072

17110

19670

5H77833-2times1305

1700

1842

28412128

1427

6228

9134

5596

570

579

813

654

5800

4267

3333

4467340

426

451

406

19816

19444

16037

18432

6H77833-2times

(77371times

BSEC

TCP

-1)

1453

1515

3120

2029

789

465510428

5291

211

408

858

492

5233

4167

3267

4222

203

283

267

251

20677

1913

816470

1876

2

7H77833-2times

96AC

-93

1835

1783

2427

2015

2532

6649

1610

684

29

350

650

1473

824

5467

4233

3700

4467243

239

256

246

22278

20434

15735

19482

8H77833-2times

Togo-II

1564

15632500

1876

1882

4772

11660

6105

296

469

972

579

5667

4533

440048

67

169

260

211

213

2412

82375

619389

22424

9H77833-2times

99HS-18

159318002049

1814

1571

480214024

6799

203

544

1547

765

6033

460040

0048

78

261

225

256

247

23466

21081

1793

320

827

10H7729-2times

G73-107

14951568

2075

1713

959

5157

8517

4877

218

382

666

422

6133

5067

4833

5344

300

273

239

271

23651

2191

717083

20884

11H7729-2times77245

1557

1537

2613

1902

1851

5756

5729

4445366

562

524

484

5633

4800

4633

5022

133

233

297

221

1979

219914

15250

18319

12H7729-2times

CVJ-2-5-3-1-3

18442033

28932257

1651

806114885

8199

264

597

1054

638

6333

4600

4233

5056

170

211

217

199

24629

23339

1970

022556

13H7729-2times1305

1325

1677309920

34

1756

724314048

7682

318

635

1232

728

630046

67

3633

4867298

261

361

307

21666

19371

17422

19486

14H7729-2times(77371

timesBS

ECTCP

-1)

1958

1887

3105

2317

1118

4675

14411

6734

242

457

1318

672

5900

4500

3800

4733

164

209

240

204

23661

1995

419250

20955

15H7729-2times

96AC

-93

1512

1717

2779

2002266844

08

11393

6156

356

500

1211

689

5633

4533

4200

4789

221

195

282

232

2194

921635

17213

20266

Advances in Agriculture 9

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

16H7729-2times

Togo-II

1519

1738

2159

1805

2437

9867

1616

294

89

361

961

1527

950

6167

4700

4200

5022

234

272

230

246

26338

2414

520553

23678

17H7729-2times

99HS-18

1938

1787

3151

2292

1574

6218

11318

6370

290

606

956

617

6067

4767

406749

67

172

210

199

194

24689

21854

1778

921444

18G73-107times77245

1856

1813

2210

1960

2435

8550

7828

6271338

633

612

528

5500

4633

440048

44

199

255

307

254

1993

619277

16095

18436

19G73-107times

CVJ-2-5-3-1-3

1517

1769

2407

1897

1556

7728

9136

6140

239

876

917

677

5900

4300

3400

4533

233

266

315

272

23418

22333

19317

21689

20G73-107times1305

1217

1507

2832

1852

1777

5189

1114

960

38

289

588

1197

691

6733

5000

4233

5322

286

210

312

269

2078

81992

817067

19261

21G73-107times(77371

timesBS

ECTCP

-1)

18551946

2730

2177

1801

3482

10415

5233

239

277

753

423

5500

4467

3433

4467243

197

273

238

23817

20263

17425

20502

22G73-107times

96AC

-93

1718

1840

28612140

1345

9333

14669

8449

297

845

1278

807

5600

5033

4633

5089

270

243

300

271

28537

21461

16875

22291

23G73-107timesTo

go-II

168018083087

2192

1112

4678

9319

5036

186

473

985

548

6033

4767

4200

5000

210

209

231

217

24864

24609

21625

23699

24G73-107times99HS-18

1398

1675

3370

2148

2753

8672

11918

7781

362

691

981

678

566746

00

3500

4589

170

247

270

229

24677

20567

17855

21033

2577245times

CVJ-2-5-3-1-3

1500

1728

2203

1810

2282

4679

11492

6151

328

424

1116

623

6000

4333

420048

44300

231

370

300

21257

20497

1772

519826

2677245times1305

143815772080

1698

949

10439

12053

7814

121

915

992

676

5767

4800

3500

4689

168246

316

244

1978

91779

015517

17699

2777245times(77371times

BSEC

TCP

-1)

150516972846

2016

3222

8518

12621

8120

469

827

1141

812

5400

4367

3833

4533

196

225

242

221

2092

819485

1719

219202

2877245times96AC

-93

1653

1797

2117

1856

1934

6056

11543

6511

352

563

911

608

5333

4267

3833

4478

186

169

198

184

22003

19054

1676

719275

2977245timesTo

go-II

1723

1718

3163

2201

1749

8250

15443

8481229

806

1412

816

5967

4700

4067

4911

174

245

298

239

22075

23503

19875

21818

3077245times99HS-18

2016

1970

28542280

3217

13027

3531

6591395

965

285

549

5733

4467

3833

4678

148

228

209

195

2212

721462

1690

020

163

31CV

J-2-5-3-1-3times

1305

1358

1422

2179

1653

1254

2803

1019

44750

178

254

801

411

6033

4467

3367

4622

312

193

330

278

22269

19547

17250

19689

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

1335

1742

2400

1826

922

5226

12806

6318

236

458

1054

583

5633

4433

3600

4556

321

315

259

298

2210

419524

17087

19572

33CV

J-2-5-3-1-3times

96AC

-93

1552

16803260

2164

166710094

11941

7901250

974

1115

779

5533

4533

446748

44

170

202

317

230

2392

123317

19333

2219

0

34CV

J-2-5-3-1-3times

Togo-II

142216072638

1889

1012

4163

11061

5412

261

377

891

510

6167

4967

4533

5222

207

225

325

252

23590

22860

20278

22243

35CV

J-2-5-3-1-3times

99HS-18

1435

1600

2700

1912

3056

3237

7089

4461449

301

658

469

5700

44673800

4656

148

178

225

183

22444

20240

1696

719884

361305times(77371times

BSEC

TCP

-1)

1499

1767

2058

1774

1882

4665

7920

4822

356

426

724

502

566742673800

4578

271

242

305

273

22011

19295

16417

19241

371305times96AC

-93

16781605

2757

2014

1516

10037

11960

7838

311

11381284

911

560040

673400

4356

182

276

251

236

2396

519625

15800

1979

738

1305timesTo

go-II

1177

16802249

1702

902

4676

9388

4989

241

456

795

497

6533

5533

4733

5600

214

266

296

259

21894

20217

19222

20444

391305times99HS-18

1774

1767

2766

2102

2145

4628

10618

5797

301

447

991

580

5933

4500

3733

4722

128

142

180

150

2275

719413

16967

1971

2

40(77371times

BSEC

TCP

-1)times

96AC

-93

1748

1795

31712238

1537

12586

14379

9501

211

1104

1183

833

546744

00346744

44

127

201

226

185

23294

21089

17858

2074

7

10 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(

days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

41(77371times

BSEC

TCP

-1)times

Togo-II

19652007

2653

2208

2136

5601

9503

5747

315

635

1001

650

5533

4833

410048

22

213

241

286

247

22662

22094

17667

20808

42(77371times

BSEC

TCP

-1)times

99HS-18

15011687

2705

1964

1935

7253

11806

6998

314

704

1067

695

5467

4433

5133

5011

177

167

175

173

22305

19277

16392

19325

4396AC

-93timesTo

go-II1759

1618

2123

1834

1539

10347

12212

8033

325

907

1090

774

5900

450039674789

214

269

339

274

23252

21554

16875

20560

4496AC

-93times

99HS-18

1353

1618

2925

1966

3532

7223

11950

7569

440

654

1024

706

5600

4900

4533

5011

149

175

206

177

24494

20698

18111

2110

145

Togo-IItimes

99HS-18

1117

1278

2582

1659

3448

6764

11910

7374

477

767

1207

817

6000

5133

4333

5156

183

203

216

201

17683

15541

14333

15853

Mean

1513

1618

2492

1874

1656

5773

9854

5761

272

538

885

565

586846

534116

4879

218

233

268

240

21297

19547

16529

1912

4SE

101

073

098

mdash079

152

317

mdash014

014

028

mdash351

365

428

mdash036

039

046

mdash985

1173

1211

mdashCD

284

205

276

mdash221

427

890

mdash038

040

079

mdash12

513

015

2mdash

013

014

016

mdash351

418

431

mdash

(c)

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

1642

1667

1744

1684

1504

1349

1526

1460

2783

2405

2634

2607

836

745

908

830

4287

3754

4160

4067

2H7729-2

1693

1817

1950

1820

1012

859

1016

962

3433

2809

2591

2944

649

597

651

632

4445

3668

3607

3906

3G73-107

2000

2028

2077

2035

2280

1966

2312

2186

4695

4023

4229

4316

1281

1170

1463

1305

6975

5989

6541

6502

477245

1523

1630

1728

1627

1796

2489

2702

2329

5423

4851

4498

4924

748

1464

1743

1319

7219

7341

7200

7253

5CV

J-2-5-3-1-3

2282

2692

2787

2587

2411

2643

2901

2652

5857

4836

5024

5239

1206

1335

1769

1437

8268

7479

7925

7891

61305

1851

1951

2054

1952

2026

1763

1908

1899

4657

4414

4463

4511

1468

1336

1539

1448

6683

6177

6371

6410

777371timesBS

ECTCP

-11629

1645

1733

1669

1244

1692

1884

1607

4925

4183

4435

4515

625

1092

1483

1067

6169

5875

6319

6121

896AC

-93

1379

1519

1600

1499

2066

2942

3088

2698

4501

4095

4229

4275

984

1565

1678

1409

6567

7037

7316

6973

9To

go-II

1904

2057

2103

2021

2167

1844

2358

2123

4520

3446

3904

3957

1298

1138

1502

1313

6687

5290

6262

6080

1099HS-18

1475

1485

1633

1531

3180

2904

3382

3155

5633

4139

5205

4992

1656

1536

1879

1690

8813

7043

8587

8147

1H77833-2timesH7729-2

2010

2046

2133

2063

3645

3075

4377

3699

7675

6540

7199

7138

2014

1708

2880

2201

11320

9615

11576

10837

2H77833-2timesG73-107

2066

2101

2122

2096

5002

4053

4601

4552

7882

7430

6963

7425

3146

2565

2808

2840

12884

11482

11564

11977

3H77833-2times77245

1764

2048

2083

1965

3769

4497

4559

4275

8681

8515

8475

8557

2284

3356

3377

3006

12450

13012

13034

12832

4H77833-2times

CVJ-2-5-3-1-3

2205

2383

2733

2441

3693

5655

5220

4856

11656

10287

1010

510683

2198

4488

4176

3621

15348

1594

215325

15538

5H77833-2times1305

2209

2423

2491

2374

6008

5842

6933

6261

15238

14311

13889

14479

3758

4057

5591

4469

21246

2015

320822

2074

0

6H77833-2times(77371times

BSEC

TCP

-1)

1789

1823

1992

1868

3593

4288

4903

4262

8922

6522

7429

7625

2178

3224

3070

2824

12515

10811

12333

11886

7H77833-2times96AC

-93

1739

1960

2011

1903

4557

4350

4818

4575

7916

6068

7290

7092

2411

2529

3191

2710

12474

10419

1210

811667

8H77833-2timesTo

go-II

1927

2389

2406

2241

4185

5828

6215

5409

12319

10279

11023

11207

2433

4254

4631

3773

16504

1610

717238

16616

9H77833-2times99HS-18

1941

2245

2330

2172

4227

4139

4529

4298

9211

8120

7969

8433

3154

3184

3253

3197

13438

12259

12498

1273

210

H7729-2timesG73-107

1968

2109

2158

2079

3725

3042

3488

3418

7313

5956

6174

6481

2822

2001

2357

2393

11038

8998

9662

9899

Advances in Agriculture 11

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

11H7729-2times77245

1847

2048

2200

2032

2467

3759

5466

3898

7672

7449

8921

8014

1356

2350

3796

2500

1013

911208

14387

11911

12H7729-2times

CVJ-2-5-3-1-3

2218

2585

2767

2523

3674

4372

4483

4176

10053

9467

8664

9395

2417

3312

3345

3025

1372

813839

1314

713571

13H7729-2times1305

2169

2338

2547

2352

6728

6166

6792

6562

13333

11662

13301

1276

54128

4501

5660

4763

20061

17828

20093

19327

14H7729-2times(77371times

BSEC

TCP

-1)

1925

2095

2127

2049

3530

4165

4649

4115

9638

6917

7698

8084

1681

2833

3150

2555

1316

8110

82

12347

1219

915

H7729-2times96AC

-93

2012

2070

2180

2087

4955

4447

4596

4666

8738

6882

7762

7794

3754

3558

3181

3498

13693

11329

12359

12460

16H7729-2timesTo

go-II

2138

2428

2563

2377

4949

4660

5118

4909

8549

7243

7191

7661

3586

3353

3028

3322

13498

11903

12310

12570

17H7729-2times99HS-18

1899

1933

2073

1969

4046

4527

4988

4520

8492

7895

8144

8177

2561

3378

3553

3164

12539

12422

1313

212698

18G73-107times77245

1840

2253

2437

2177

2580

4776

5915

4424

11673

10608

10459

10913

1358

3163

4286

2936

14253

15385

16374

15337

19G73-107times

CVJ-2-5-3-1-3

2324

2537

2691

2517

4958

5030

5599

5196

9524

9395

1114

610022

3241

3645

4444

3776

14483

14425

1674

515217

20G73-107times1305

2228

2433

2583

2415

6340

5794

6373

6169

12490

1015

8119

61

11536

4199

4199

5018

4472

18830

1595

218334

1770

6

21G73-107times(77371times

BSEC

TCP

-1)

1943

2128

2220

2097

3936

3980

5770

4562

11697

9858

10303

10619

1874

2369

4181

2808

15633

13839

16073

1518

222

G73-107times96AC

-93

2118

2218

2317

2217

4270

5843

6201

5438

10319

9872

1015

910117

2703

4460

4379

3847

14589

15715

16359

15555

23G73-107timesTo

go-II

2237

2365

2733

2445

5736

5175

6822

5911

12098

10808

1299

611967

3984

3670

5372

4342

17835

1598

219818

17878

24G73-107times99HS-18

2025

2081

2322

2143

3558

4229

6612

4800

12350

9685

11516

1118

42196

2782

5009

3329

1590

813914

1812

915983

2577245timesCV

J-2-5-3-1-3

2109

2360

2675

2381

6760

6558

7299

6872

15225

11861

1416

21374

94225

4752

5839

4939

2198

518418

21461

20621

2677245times1305

1919

2100

2197

2072

5700

5142

5347

5397

11687

10565

10737

10996

4318

3956

4244

4173

17386

1570

816085

16393

2777245times(77371times

BSEC

TCP

-1)

1851

1935

2020

1936

2577

2460

2838

2625

6989

4778

4636

5468

1631

1662

1999

1764

9566

7238

7474

8093

2877245times96AC

-93

1729

1780

2133

1881

5541

5058

5748

5449

11952

8174

9526

9884

3646

3587

4071

3768

17493

13232

15274

15333

2977245timesTo

go-II

2300

2508

2658

2489

3067

5431

5963

4820

11737

11021

1216

411641

1598

4053

4770

3474

14805

16452

1812

616461

3077245times99HS-18

1982

2019

2223

2075

3846

3119

4225

3730

7493

6720

6476

6896

3205

2578

2643

2809

11339

9839

1070

110626

12 Advances in Agriculture

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

31CV

J-2-5-3-1-3times1305

2406

2553

3067

2675

4178

4796

6281

5085

13822

11425

12252

12500

2472

3241

4907

3540

18000

16222

18532

17585

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

2142

2173

2238

2184

4522

5235

5640

5132

11673

9658

10248

10526

2614

3766

4178

3519

1619

514893

15888

15659

33CV

J-2-5-3-1-3times

96AC

-93

2350

2477

2600

2476

4676

4280

6090

5015

11975

9832

10868

10891

3097

2931

4445

3491

16651

14111

1695

815907

34CV

J-2-5-3-1-3times

Togo-II

2093

2666

2989

2583

4058

3727

5190

4325

12588

10717

11217

11507

2742

2570

4185

3166

16645

14443

16407

15832

35CV

J-2-5-3-1-3times

99HS-18

2028

2311

2386

2242

3287

2513

5356

3719

10055

9733

1036

410051

2004

1581

4251

2612

13342

12246

1572

01376

9

361305times(77371times

BSEC

TCP

-1)

2005

2173

2210

2129

3569

4692

5140

4467

11652

9826

10041

10507

2005

3665

4179

3283

15221

14518

1518

214974

371305times96AC

-93

1822

2003

2047

1957

5115

4023

4813

4650

9965

7675

7571

8404

3456

2647

3155

3086

15080

11698

12384

13054

381305timesTo

go-II

2545

2583

2644

2591

6885

7283

6795

6987

12265

11410

12583

12086

4500

6120

5350

5323

1915

018693

19378

19074

391305times99HS-18

2070

2408

2593

2357

5219

4332

4800

4784

1092

97677

8968

9192

3924

3332

3840

3699

1614

912009

1376

913976

40(77371times

BSEC

TCP

-1)

times96AC

-93

2056

2115

2157

2109

3158

4463

4717

4113

9166

7540

8100

8268

1880

3542

3347

2923

12324

12003

12817

12381

41(77371times

BSEC

TCP

-1)

timesTo

go-II

1868

2253

2450

2190

4422

6458

7038

5973

1415

312369

1379

713439

2291

4646

5586

4174

18575

18827

20835

19412

42(77371times

BSEC

TCP

-1)

times99HS-18

1995

2026

2209

2077

2720

2909

3543

3057

7392

4692

5023

5702

1766

2187

2147

2033

10112

7601

8566

8760

4396AC

-93timesTo

go-II

1953

2027

2511

2164

5972

4391

7126

5830

15537

1392

714432

14632

3780

2538

5655

3991

21510

18318

21558

20462

4496AC

-93times99HS-18

1952

2021

2242

2072

3465

2758

4496

3573

5959

5186

6094

5746

2707

2154

2409

2423

9424

7944

10590

9319

45To

go-IItimes

99HS-18

1932

2008

2658

2199

5381

4995

5436

5270

10299

8876

9244

9473

3986

3842

3668

3832

15680

13871

14680

1474

3Mean

1982

2146

2301

2143

3963

4123

4836

4307

9498

8087

8663

8749

2510

2914

3513

2979

13460

12210

13499

13056

SE243

198

245

mdash671

501

626

mdash959

750

822

mdash423

349

344

mdash1155

927

1167

mdashCD

087

071

087

mdash239

178

223

mdash342

267

293

mdash15

112

412

2mdash

411

330

416

mdash

Advances in Agriculture 13Ta

ble4Analysis

ofvaria

ncefor

10times10

half-diallelfor

different

charactersin

threee

nviro

nments

(a)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Germination

()

Rateof

emergence

STI()

SSTI

()

Num

bero

fleavesseedling

Seedlin

gheight

(cm)

Freshweight

(g)seedlin

gDry

weight

(g)seedlin

g

E 1

Replicates

2652731

596768

783393

20700

93018674

33764

1033999

002590

Treatm

ents

541097

0255lowastlowast

8181153lowastlowast

30863092lowastlowast

20680900lowastlowast

1100708lowastlowast

2106317lowastlowast

930829527lowastlowast

319767lowastlowast

Parents

95118

163lowastlowast

14289925lowastlowast

59114816lowastlowast

18880254lowastlowast

1084227lowastlowast

1087906lowastlowast

22561719lowastlowast

042162lowastlowast

Hybrid

s44

11423573lowastlowast

3398933lowastlowast

25697199lowastlowast

20633716lowastlowast

472874lowastlowast

1391526lowastlowast

674123518lowastlowast

261839lowastlowast

Parentsv

ersush

ybrid

s1

43693104lowastlowast

163619

916lowastlowast

3896838

3896

2793lowastlowast

28873741lowastlowast

4272

2798lowastlowast

234144289lowastlowast

5367022lowastlowast

Error

108

281184

1356832

1173684

674337

040104

306

462

2015

1619

005535

E 2

Replicates

2583819

3707389lowast

1596635

198738

034925

448213

899529

007177

Treatm

ents

549997443lowastlowast

2825177lowastlowast

35378177lowastlowast

19074306lowastlowast

665284lowastlowast

1764

081lowastlowast

257694570lowastlowast

2177519lowastlowast

Parents

96507784lowastlowast

3718

879lowastlowast

69866289lowastlowast

1798

5404lowastlowast

705379lowastlowast

1408518lowastlowast

10612519lowastlowast

077429lowastlowast

Hybrid

s44

10569731lowastlowast

2127302lowastlowast

2910

2537lowastlowast

19429240lowastlowast

329256lowastlowast

7000

03lowastlowast

186026552lowastlowast

1483428lowastlowast

Parentsv

ersush

ybrid

s1

16223710lowastlowast

25488361lowastlowast

1113

326

13257362lowastlowast

15089673lowastlowast

5178

3569lowastlowast

5634825791lowastlowast

51618348lowastlowast

Error

108

334760

1097039

1049505

635570

06764

116

0576

694613

006206

E 3

Replicates

2894398

4808815lowast

mdashmdash

051076

095989

3768421

030220

Treatm

ents

541261560

0lowastlowast

4842216lowastlowast

mdashmdash

2811077lowastlowast

5947316lowastlowast

3870844

50lowastlowast

3445076lowastlowast

Parents

97135

735lowastlowast

1097

6388lowastlowast

mdashmdash

1468699lowastlowast

1112

764lowastlowast

136081650lowastlowast

1307836lowastlowast

Hybrid

s44

13217740lowastlowast

1379360

mdashmdash

15540

71lowastlowast

4581632lowastlowast

252084767lowastlowast

2378032lowastlowast

Parentsv

ersush

ybrid

s1

35440

180lowastlowast

102000335lowastlowast

mdashmdash

70200767lowastlowast

109548362lowastlowast

8586095705lowastlowast

69630188lowastlowast

Error

108

782907

1085444

mdashmdash

139771

291325

3019

169

02400

4(b)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Panicle

emergence

Effectiv

etillersplant

Planth

eight

Earlength

Earw

eightp

lant

Dry

fodd

eryieldplant

Grain

yieldplant

Totalbiological

yieldplant

E 1

Replicates

231860

61lowastlowast

416348lowastlowast

1672

9472lowast

1706

411077192

9634539

392777

115544

59Treatm

ents

544152

368lowastlowast

085657lowastlowast

326706089lowastlowast

1602572lowastlowast

6177

0406lowastlowast

304113

204lowastlowast

34285030lowastlowast

575617

582lowastlowast

Parents

94511481lowastlowast

018538lowastlowast

140807476lowastlowast

2240093lowastlowast

11653

959lowastlowast

27014155lowastlowast

3858650lowastlowast

61321919lowastlowast

Hybrid

s44

3201347lowastlowast

100797lowastlowast

110395657lowastlowast

1010

497lowastlowast

40296671lowastlowast

171271411lowastlowast

2413

6880lowastlowast

302999650lowastlowast

Parentsv

ersush

ybrid

s1

4276

5286lowastlowast

02360

9lowast11517452

616lowastlowast

2191

6167lowastlowast

1457662749lowastlowast

8643043524lowastlowast

7546

41053lowastlowast

17199467546lowastlowast

Error

108

468159

004907

3692099

224610

17114

303498126

682350

5075268

E 2

Replicates

2656364

002556

61575565lowastlowast

828842lowastlowast

589094

2268506

260877

1507116

Treatm

ents

542999618lowastlowast

069822lowastlowast

268998723lowastlowast

2436886lowastlowast

60592784lowastlowast

247259877lowastlowast

4192

0073lowastlowast

513483489lowastlowast

Parents

926300

00lowastlowast

048079lowastlowast

165555

405lowastlowast

3871874lowastlowast

1412

2393lowastlowast

19543682lowastlowast

3132

426lowastlowast

57230706lowastlowast

Hybrid

s44

2533

165lowastlowast

07344

9lowastlowast

9616

2857lowastlowast

1463359lowastlowast

35495366lowastlowast

154759288lowastlowast

26262465lowastlowast

293492304lowastlowast

Parentsv

ersush

ybrid

s1

26850135lowastlowast

105929lowastlowast

880476

6697lowastlowast

32357161lowastlowast

1583112746lowastlowast

6366731560lowastlowast

107994

3646lowastlowast

14299370699lowastlowast

Error

108

507598

005829

5234122

149406

955133

2142411

462311

3273886

E 3

Replicates

2029697

021161

9459847

143746

985141

46800

6492

6334

3345160

Treatm

ents

548045499lowastlowast

094511lowastlowast

192420108lowastlowast

3279225lowastlowast

72049211lowastlowast

298953

021lowastlowast

55061308lowastlowast

651732994lowastlowast

Parents

98010

741lowastlowast

094166lowastlowast

116037343lowastlowast

3702185lowastlowast

16305190lowastlowast

2323644

0lowastlowast

4553

893lowastlowast

71484982lowastlowast

Hybrid

s44

6254377lowastlowast

095514lowastlowast

7378

1513lowastlowast

2188348lowastlowast

31813193lowastlowast

190263916lowastlowast

31572179lowastlowast

368689934lowastlowast

Parentsv

ersush

ybrid

s1

8716

7710lowastlowast

053496lowast

609996

3199lowastlowast

47471212lowastlowast

2344

130203lowastlowast

756272

2861lowastlowast

154314

9707lowastlowast

18327859

719lowastlowast

Error

108

696981

007883

5578505

228608

1491014

2574

223

449588

5187705

lowast

Sign

ificant

at119875=005

lowastlowastSign

ificant

at119875=001

14 Advances in Agriculture

Table 5 Mean performance of the genotypes for membrane thermostability in two environments

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594

Advances in Agriculture 15

Table 5 Continued

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434

Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash

lowastValues are transformed values

0

6

12

18

24

30

36

42

48

54

minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity

G73-10796AC-93CVJ-2-5-3-1-3

Togo-II99HS-18

H77833-2H7729-2772451305

H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305

1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305

H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305

77245 times 1305

77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305

77371 times BSECT CP-1

H77833-2 times G73-107

Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits

moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling

survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The

16 Advances in Agriculture

Table6Po

oled

correla

tioncoeffi

cientamon

gvario

uscharactersin

threee

nviro

nments

Characters

Germination

Rateof

emergence

STI

SSTI

Leaves

seedlin

gSeedlin

gheight

Fresh

weight

Dry

weight

MTS

Panicle

emergence

Effectiv

etillersplant

Plant

height

Earlength

Ear

weightp

lant

Dry

fodd

eryield

plant

Grain

yield

plant

Total

biological

yield

plant

Germination

1000

0357lowastlowast

0353lowastlowast

0590lowastlowast

0230

0373lowastlowast

0293lowast

0260

0276lowastminus0135minus0478lowastlowast

0326lowast

minus0069minus0104

minus0190

minus0145

minus0167

Rateof

emergence

1000

0233

0351lowastlowast

0605lowastlowast

0585lowastlowast

0551lowastlowast

0524lowastlowast

0139minus0593lowastlowast

0032

0517lowastlowast

0217

0388lowastlowast

0361lowastlowast

0348lowastlowast

0377lowastlowast

STI

1000

0837lowastlowast

0241

0129

0081

004

80890lowastlowast

minus0215

minus0211

0030minus0007minus0043

minus0101

minus0111

minus0084

SSTI

1000

0233

0264

0183

0140

0745lowastlowast

minus0247minus0268lowast

0184minus0017minus006

6minus0129

minus0144

minus0112

Num

bero

fleaves

1000

0683lowastlowast

0633lowastlowast

060

4lowastlowast

0176minus0474lowastlowast

0109

0576lowastlowast

0496lowastlowast

064

1lowastlowast

0607lowastlowast

0630lowastlowast

0630lowastlowast

Seedlin

gheight

1000

0703lowastlowast

0663lowastlowast

0031minus0493lowastlowast

minus0115

0735lowastlowast

0360lowastlowast

0526lowastlowast

0520lowastlowast

0515lowastlowast

0532lowastlowast

Freshweight

1000

0957lowastlowast

minus0016

0425lowastlowast

minus0082

0661lowastlowast

0351lowastlowast

0581lowastlowast

0496lowastlowast

0576lowastlowast

0533lowastlowast

Dry

weight

1000minus0033minus0428lowastlowast

minus0016

0634lowastlowast

0354lowastlowast

0620lowastlowast

0509lowastlowast

0614lowastlowast

0554lowastlowast

MTS

1000minus040

minus0177minus0037

0015

minus0074

minus0138

minus0131

minus0120

Panicle

emergence

1000

minus0150minus0269lowast

0022minus0329lowast

minus0337lowast

minus0280lowast

minus0341lowast

Effectiv

etillersplant

1000

minus0012

0170

0366lowastlowast

040

5lowastlowast

0323lowast

0401lowastlowast

Planth

eight

1000

0650lowastlowast

0603lowastlowast

0596lowastlowast

0619lowastlowast

0610lowastlowast

Earlength

1000

0608lowastlowast

0671lowastlowast

064

8lowastlowast

066

4lowastlowast

Earw

eightp

lant

1000

0910lowastlowast

0984lowastlowast

0957lowastlowast

Dry

fodd

eryield

plant

1000

0907lowastlowast

0991lowastlowast

Grain

yield

plant

1000

0950lowastlowast

Totalbiological

yield

plant

1000

lowastSign

ificant

at119875=005lowastlowastSign

ificant

at119875=001

Advances in Agriculture 17

faster germination and early vigour in seedling could act asphysical indicators for heat tolerance

Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits

Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics

Conflict of Interests

The authors declare that there is no conflict of interestsregarding the publication of this paper

References

[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981

[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993

[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006

[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993

[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013

[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007

[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965

[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011

[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001

[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009

[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962

[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967

[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963

[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001

Submit your manuscripts athttpwwwhindawicom

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GenomicsInternational Journal of

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Page 3: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

Advances in Agriculture 3

0

10

20

30

40

50

60

70

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15DAS

E1

E2

E3

Tem

pera

ture

(∘C)

(a)

05

101520253035404550

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15DAS

E1

E2

E3

Tem

pera

ture

(∘C)

(b)

0

10

20

30

40

50

60

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16DAS

E1

E2

E3

Tem

pera

ture

(∘C)

(c)

02468

101214161820

1 3 5 7 9 11 13 15

Moi

sture

()

DAS

E1

E2

E3

Permanent wilting point

(d)

Figure 1 (a) Soil surface temperature (b) air temperature (c) temperature 5 cm above the soil surface and (d) soil moisture status in differentenvironments

30 cm apart with 10 cm plant to plant spacingThe seeds weresown manually

The soil of experimental field was sandy loam in textureThe water holding capacity field capacity and permanentwilting point of the experimental field were 40 17 and 6percent respectively [6] Irrigation was withheld during thecourse of experiment however drought stress-free condi-tions were ensured through monitoring at regular interval(on alternate days) by measuring the moisture status of thesoil by gravimetric method The soil samples of about 50 gwere taken up to 20 cmdepth from the experimental plotThemoisture content on dry weight basis may be calculated [7]using the following formula

Soil moisture ()

=

(Weight of wet soil minusWeight of dry soil)Weight of dry soil

times 100

(1)

The heat stress period was terminated by irrigating theexperimental field before reaching the soil moisture level atpermanent wilting point A condition of no drought wasmaintained in order to determine the exclusive effect of tem-perature at seedling stageThis was maintained by measuringthe moisture status of the soil by gravimetric method onalternate days The soil surface temperature was measuredby a soil thermometer at soil surface temperature at 5 cmabove the soil surface and air temperature at 1m above thesoil surface were recorded between 200 pm and 230 pmdaily (Figure 1)

Emphasis of observation is centred on germination sur-vival and mortality of the seedlings

23 Plant CharacteristicsScreening Techniques The empha-sis was given to the observations of germination survival andmortality of the seedlingsThe seedlings were inspected everyday in the morning The seedlings which had died due to

4 Advances in Agriculture

heat stress were noted in each genotype on daily basis Obser-vations continued till the surviving seedlingswere established(15 DAE) and there was no further mortality Moreover thesoil moisture was depleting near to the permanent wiltingpoint and drought stress could also come into play along withheat stress to further continue the experiment Since experi-ment proceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedlingsurvival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of heat stress at seedling stage was observed on latergrowth stages

231 Thermotolerance Characteristics For heat tolerancecharacteristics 3 indices namely seedling thermotoleranceindex (STI) [4] seed to seedling thermotolerance index(SSTI) [8] and membrane thermostability (MTS) wereapplied to evaluate the hybrids and parents STI was cal-culated as the ratio of number of seedlings survived to thenumber of seedling emergences expressed in percentageSeed to seedling thermotolerance index (SSTI) was calculatedas the ratio of seedling survival to the number of seedlingsexpected to emerge expressed in percentage [8] The SSTIis an extension of STI by taking expected germination intoaccount It was necessary to correct the effect of under soilmortality (USM) The loss of germination due to heat (ieunder soil mortality) can be measured only after a standardgermination test Therefore the germination of seed fromthe same lot under monsoon environment (main pearl milletgrowing season where no under soil mortality occurreddue to heat stress) was taken as expected germination forcalculation of SSTI

232 Membrane Thermostability These genotypes were alsoscreened through laboratory technique (membrane ther-mostability) [9 10] The young leaves of 21-day-old seedlingsof each genotype in three replication plots were used formembrane thermostability test A middle portion of 10 cmfrom each leaf was taken and the midrib was removed andtwo equal halves of the 10 cm each were made Each halfwas taken to provide replications Each leaf portion was cutinto pieces and thoroughly washed and rinsed with distilledwater two times to remove the electrolytes adhering to theplant tissue as well as electrolyte released from the cutting ofplant tissue and put them into test tubes After final rinsingtubes were drained maintaining sufficient water to preventdesiccation of plant material during heat treatment Thesetest tubes were covered with aluminium foil and incubated inwater bath at 55∘C for 15 minutes for giving heat treatmentAfter the heat treatment period 10mL of distilled waterwas added to the test tubes Then all the test tubes wereheld at 10∘C overnight to allow diffusion of electrolytes fromplant material Test tubes were brought out of refrigeratorand shaken well to mix the contents An initial electricalconductivity (EC) of both replications of each treatment (119879

1)

was determined with electrical conductivity meter Thenthese test tubes were autoclaved at 15 psi for 2 minutes forcomplete electrolyte leakage from the leaf samples These testtubes were then cooled at room temperature and contentsmixed and final EC was taken for all the treatments (119879

2)

Membrane thermostability (MTS) was calculated accordingto Ibrahim and Quick [9] and Yadav et al [10] as follows

Membrane Thermostability (MTS) = (1 minus1198791

1198792

) times 100

(2)

where 1198791is conductivity reading after heat treatment and 119879

2

is conductivity reading after autoclavingCompletely randomized design (CRD) for membrane

thermostabilitywas conducted to detect variations among thegenotypes

233 Morphological Characteristics The seedling growthcharacteristics namely germination () emergence rate(ER) number of leavesseedling (two-week stage) seedlingheight (two-week stage) seedling fresh weight (g) (four-week stage) and seedling dry weight (g) (four-week stage)were recorded The data for seedling height and number ofleaves per seedling was recorded on 10 random seedlings perplot Emergence rate (an index for speed of germination)can be used as a tool in pearl millet breeding programs forevaluation of seedling vigour Speed of germination is oneof the oldest concepts of seedling vigour The emergencerate (ER) was calculated by dividing the number of normalseedlings obtained at each counting by the number of daysseeds had been in the seed bed (DAS)The values obtained ateach count were then summed at the end of the germinationtest to obtain the emergence rate [11]

Emergence Rate (ER) =Seedling count

Days to first count+ sdot sdot sdot

+

Seedling countDays to final count

(3)

After termination of the heat stress on the seedlings byirrigating the field the experiment was continued to studythe effect of seedling heat stress on maturity traits on thesurvived plants The maturity traits observed were panicleemergence effective tillersplant plant height ear length earweightplant dry fodder yieldplant grain yieldplant andtotal biological yieldplant

24 Statistical Analysis Analysis of variance for randomizedblock design (RBD) for field traits that is germinationemergence rate number of leavesseedling seedling heightseedling fresh weight and seedling dry weight and com-pletely randomized design (CRD) for membrane thermosta-bility test was conducted under each environment on meanbasis [12] Analyses were done using the software OPSTATdeveloped at CCS HAU Hisar Correlations among the vari-ous characters studied were calculated to establish the natureand magnitude of associations among the traits [8 13] with

Advances in Agriculture 5

the software SPSS (SPSS Inc) Multivariate clustering wasdone based on paired group cluster method using similaritycoefficient under Past 140 software [14] Multivariate analysisbased on the Euclidean distancematrix was estimated amonggenotypes to distinguish genotypes from each other Thegrouping of genotypes was generated using paired groupcluster method

3 Results and Discussion

The excessive heat (soil temperature up to 630∘C in E2)

about 18∘C higher than the normal atmospheric temperature(Figures 1(a) and 1(b)) was responsible for mortality ofseedlings Many seedlings were found desiccated at the pointof contact besides the desiccation at the tipsThe temperaturearound the tip level (5 cm above the soil surface) also reachedup to 50∘C (Figure 1(c)) It could therefore be concluded thatthe seedlings had to survive in a really high temperature Themoisture level remained above the permanent wilting point(Figure 1(d)) therefore drought effect was not in the playThe heat tolerance was measured as STI (Table 3) throughthe field screening technique of Peacock et al [4] Anotherindex known as SSTI was also calculated as an improvementand extensionThe analysis of variance (RBD) for 8 charactersin 3 environments showed significant genotypic differencesNo variability was observed for STI and SSTI in E

3only In

this environment no seedling mortality was observed andthe germination was taken as standard The values for STIand SSTI were therefore equal and the highest for all thegenotypes in this environment and hence no variation wasobserved

The hybrids were evaluated on the basis of mean perfor-mance for various characters in each environment (Table 3)On the mean basis STI value ranged from 5332 to 8224with an average of 7072 The hybrid H7729-2 times CVJ-2-5-3-1-3 was the best scorer for heat tolerance followed byH77833-2 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II The hybrid H77833-2 times 1305 was found susceptible toheat stress Seed to seedling thermotolerance index (SSTI)which is expected to be more rigorous index deviated thevalues from STI according to variation in germination undernormal environment (E

3) as the SSTI also coversincludes

the under surface mortality (USM) during germinationThe reductions in values were not strictly proportional tothe STI The reduction is dependent on thermosensitivityof germination We also came across such hybrids whichshowed poor germination and high STI SSTI values rangedfrom 4605 to 7287 with an average of 6090 The genotypeH77833-2 times 96AC-93 had the highest worth for SSTI onmean basis followed byG73-107times (77371timesBSECTCP-1) andH77833-2 times (77371 times BSECT CP-1) (Table 3) The analysisof variance for ten different characters in three differentenvironments (two stressed and one normal) was carried outand the results showed significant genotypic differences forall the characters (Table 4)

A caution is needed for evaluating such genotypes underSTI The SSTI gave a highly reduced value putting thesecrosses in the susceptible list It may be concluded therefore

that SSTI is more dependable and desirable tool for screeningand evaluating the genotypes for heat tolerance The germi-nation was lower in E

2than in E

1 This period has the highest

range of temperature with a high average Genotype-wise thehighest germination (6209) was recorded for the genotypeH7729-2 times (77371 times BSECT CP-1) on mean basis whichgives a preliminary indication of resistance to heat while thegenotype 1times 6 had the lowest germination (3533)The hybridH77833-2 times G73-107 was adjudged as the fastest emergenceof seedlingsThe genotype (77371timesBSECTCP-1)times 96AC-93had the highest fresh weightseedlingThe genotype H7729-2 times Togo-II had the highest seedling dry weight E

1had lower

dry weightseedling than E2indicating the more heat stress

during the E1

31 Membrane Thermostability Membrane thermostability(MTS) has long been recognised as a parameter of heattolerance All the hybrids were put to membrane thermosta-bility test in the laboratory under standard protocol Acompletely randomised design (CRD) analysis was carriedout for membrane thermostability (MTS) The mean sum ofsquares due to genotypes was highly significant indicatingthat enough genetic variability was present for membranethermostability (MTS) in each environment which justifiedfurther analysis of the data The range of values lay between5073 and 7742 Among the parents H77833-2 G73-107CVJ-2-5-3-1-3 77371 times BSECT CP-1 96AC-93 Togo-II and99HS-18 were verified as resistant genotypes and H7729-2 77245 and 1305 genotypes were found susceptible asmentioned in Table 1 (on the basis of previous experiment)Among all the hybrids H7729-2 times CVJ-2-5-3-1-3 CVJ-2-5-3-1-3 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II werethree highly tolerant genotypes on the basis of membranethermostability (Table 5)

32 Correlations The pooled correlations among the charac-ters were calculated (Table 6) around heat tolerance indices(STI SSTI and membrane thermostability) with a view tofind whether there was any association of these characterswith seedling as well as maturity traits It was observedthat STI and SSTI recorded the highest significant cor-relation among themselves as they are related indices forheat tolerance Membrane thermostability also showed highsignificant correlation with STI as well as SSTI STI hada significant positive association with germination of seedonly among seedling and maturity traits studied SSTI inaddition was also positively correlated with germination andemergence rate and negatively associated with number ofeffective tillersplantMembrane thermostability also showedsignificant positive correlation with seed germination amongseedling and maturity traits studied While evaluating thediallel progenies it was also seen that the heat toleranceindices STI SSTI and membrane thermostability were notshowing any perceptible correlation with the rest of thedevelopmental traits (seedling andmaturity traits)The stressperiod was terminated by irrigating the field experiment after15 days fromdate of sowingwhen the seedlings get establishedand further mortality stopped due to heat Moreover the soil

6 Advances in Agriculture

Table3Perfo

rmance

ofthep

arentsandthec

rosses

ford

ifferentcharacters

(a)

Sr

No

Genotypes

Germination(

)Ra

teof

emergencelowast

STIlowast

()

SSTIlowast(

)Num

bero

fleavesseedlin

gE 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

3941

3737

6164

4614

4037

4109

4279

4142

7830

7387

9000

8072

4498

4133

9000

5877

661

502

800

654

2H7729-2

5513

4726

6092

5443

2815

4980

3258

3684

4835

4495

9000

6110

4441

3602

9000

5681

363

294

427

361

3G73-107

5037

4826

5918

5260

4534

4132

4481

4382

6187

5413

9000

6866

5213

4460

9000

6224

878

710

935

841

477245

4747

4624

5565

4978

3625

4604

4047

4092

4385

3580

9000

5655

3803

3040

9000

5281

377

292

437

369

5CV

J-2-5-3-1-3

4824

4832

6897

5518

5044

4907

4960

4970

7193

6813

9000

7669

4959

4785

9000

6248

802

580

923

768

61305

4326

3891

5404

4540

3376

5056

3573

4002

3903

3281

9000

5395

3211

2452

9000

4888

350

284

414

349

777371timesBS

ECTCP

-14747

4502

6320

5190

4934

5075

5065

5025

6952

6706

9000

7552

5056

4671

9000

6242

621

542

700

621

896AC

-93

4651

3818

5722

4730

4312

4915

4753

4660

6660

6001

9000

7220

5212

3920

9000

6044

701

590

899

730

9To

go-II

4613

3937

5276

4609

4245

4737

4797

4593

7198

6861

9000

7686

5980

4767

9000

6582

681

629

853

721

1099HS-18

4575

3823

6247

4882

4317

4659

4746

4574

7594

7319

9000

7971

5154

4172

9000

6109

745

554

915

738

1H77833-2timesH7729-2

5787

5295

6733

5938

4964

5015

4989

4989

6803

6504

9000

7436

5808

5134

9000

6647

711

585

780

692

2H77833-2timesG73-107

5472

4417

6405

5431

5448

5422

5239

5370

7231

6769

9000

7667

5980

4556

9000

6512

1101

705

1340

1049

3H77833-2times77245

4076

3707

5504

4429

5217

5446

5238

5300

6627

6224

9000

7284

4690

4037

9000

5909

740

525

965

744

4H77833-2timesCV

J-2-5-3-1-3

4843

4300

5856

5000

4862

4956

4913

4911

7061

6519

9000

7527

5575

4632

9000

6403

998

885

1258

1047

5H77833-2times1305

3296

2780

4521

3533

5076

4907

5238

5074

3732

3265

9000

5332

2765

2050

9000

4605

1192

934

1600

1242

6H77833-2times(77371times

BSEC

TCP

-1)5636

5539

6258

5811

4489

5219

5015

4908

6981

6482

9000

7488

6146

5680

9000

6942

898

726

1525

1050

7H77833-2times96AC

-93

5720

5515

6335

5857

4800

5186

5075

5021

7833

7786

9000

8206

6603

6256

9000

7287

1107

873

1193

1058

8H77833-2timesTo

go-II

4862

3453

5709

4675

5036

4829

4911

4925

6190

5500

9000

6897

5214

3339

9000

5851

979

670

1107

919

9H77833-2times99HS-18

4575

4418

5447

4813

5041

5074

5006

5040

5800

5073

9000

6625

4846

4133

9000

5993

978

733

1135

949

10H7729-2timesG73-107

5615

4962

6273

5617

5023

5535

5165

5241

6143

5501

9000

6881

5540

4441

9000

6327

1000

720

1363

1028

11H7729-2times77245

5472

5164

6281

5639

4745

5031

5028

4935

5572

5175

9000

6582

4901

4345

9000

6082

880

620

897

799

12H7729-2timesCV

J-2-5-3-1-3

5494

5124

6536

5718

4804

5316

4957

5026

7596

8076

9000

8224

6093

5680

9000

6924

981

932

1451

1121

13H7729-2times1305

3804

3826

5511

4380

3596

4724

4387

4236

6362

7075

9000

7479

4230

4517

9000

5916

926

725

1497

1049

14H7729-2times(77371times

BSEC

TCP

-1)

6369

4847

7412

6209

4847

5351

5246

5148

6377

6031

9000

7136

5683

4230

9000

6304

977

671

1380

1009

15H7729-2times96AC

-93

5314

4657

6702

5558

4514

5295

5102

4971

5612

4856

9000

6489

4613

3603

9000

5739

846

679

1397

974

16H7729-2timesTo

go-II

5827

5594

6651

6024

5072

5279

5271

5207

6563

5371

9000

6978

5763

4652

9000

6472

873

719

1040

877

17H7729-2times99HS-18

5741

4638

7918

6099

4847

5024

5021

4964

6179

5603

9000

6927

4882

3725

9000

5869

1012

730

1600

1114

18G73-107times77245

5115

4646

6103

5288

4977

5242

5450

5223

7006

6063

9000

7356

5685

4594

9000

6426

1021

790

1292

1034

19G73-107timesCV

J-2-5-3-1-3

5134

4894

6427

5485

4901

5317

5181

5133

6637

6124

9000

7254

5253

4710

9000

6321

1039

863

1254

1052

20G73-107times1305

4881

4561

7597

5680

4717

4791

4775

4761

6704

6380

9000

7361

4556

4115

9000

5890

1140

882

1657

1226

21G73-107times(77371times

BSEC

TCP

-1)

5636

5519

6283

5812

4986

5219

5203

5136

7990

6203

9000

7731

6620

5436

9000

7019

977

703

1395

1025

22G73-107times96AC

-93

5740

4510

6415

5555

5129

4982

5195

5102

6978

5556

9000

7178

6156

4019

9000

6392

1077

860

1264

1067

23G73-107timesTo

go-II

4460

4357

6044

4953

4396

5138

4951

4829

5279

4835

9000

6371

4018

3603

9000

5540

906

881

1189

992

24G73-107times99HS-18

5473

4356

6374

5401

5102

4955

5055

5037

6460

5197

9000

6886

5534

3704

9000

6079

1171

766

1635

1191

2577245timesCV

J-2-5-3-1-3

4766

4666

5921

5117

5170

4918

4900

4996

5928

5859

9000

6929

4785

4613

9000

6133

984

920

1194

1033

2677245times1305

4575

4060

6052

4895

5128

5297

5203

5210

5102

4990

9000

6364

3979

3464

9000

5481

1279

823

1550

1217

2777245times(77371times

BSEC

TCP

-1)

5017

4929

6022

5322

5108

5473

5274

5285

5308

5044

9000

6451

4498

4191

9000

5896

833

588

939

787

Advances in Agriculture 7

(a)Con

tinued

Sr

No

Genotypes

Germination(

)Ra

teof

emergencelowast

STIlowast

()

SSTIlowast(

)Num

bero

fleavesseedlin

gE 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

2877245times96AC

-93

5472

4705

622154

66

5135

5197

5006

5113

5247

4843

9000

6363

4708

3784

9000

5831

795

662

1058

838

2977245timesTo

go-II

5252

4492

5889

5211

5188

4861

5074

5041

4885

4263

9000

6049

4421

3379

9000

5600

977

751

1243

990

3077245times99HS-18

5740

4099

6872

5570

5249

4898

5258

5135

5628

4616

9000

6415

4863

3014

9000

5626

1045

863

1184

1031

31CV

J-2-5-3-1-3times1305

4364

3944

5700

4669

4974

4517

4650

4714

6097

5579

9000

6892

4594

3842

9000

5812

936

848

1403

1063

32CV

J-2-5-3-1-3times(77371times

BSEC

TCP

-1)4345

3808

6774

4976

5272

4996

5035

5101

6650

5766

9000

7139

4288

3381

9000

5556

800

712

1013

841

33CV

J-2-5-3-1-3times96AC

-93

4862

4197

5743

4934

4884

4980

5058

4974

7477

6760

9000

7745

5957

4709

9000

6555

933

738

1478

1050

34CV

J-2-5-3-1-3timesTo

go-II

4670

4343

6233

5082

4399

4813

4631

4614

5806

6614

9000

7140

4422

4479

9000

5967

963

855

1180

999

35CV

J-2-5-3-1-3times99HS-18

5493

5080

7273

5949

5276

4984

5162

5141

7322

6590

9000

7637

5534

4766

9000

6433

998

807

1338

1047

361305times(77371times

BSEC

TCP

-1)

4613

4539

5717

4956

4986

4955

5080

5007

5137

4870

9000

6336

4210

3936

9000

5715

863

630

913

802

371305times96AC

-93

5473

5177

6830

5827

5224

4779

4919

4974

7117

6965

9000

7694

5640

5220

9000

6620

875

694

1476

1015

381305timesTo

go-II

4230

3420

5262

4304

4309

456144

45

4438

5781

4373

9000

6385

4575

2887

9000

5487

637

566

794

666

391305times99HS-18

5433

4507

6947

5629

5225

4753

4991

4989

7287

6968

9000

7752

5576

4479

9000

6352

1115

818

1410

1114

40(77371times

BSEC

TCP

-1)times

96AC

-93

5595

4429

7461

5828

5216

4955

5121

5097

7087

6368

9000

7485

5432

4038

9000

6157

955

753

1462

1057

41(77371times

BSEC

TCP

-1)times

Togo-II

5252

4728

6106

5362

5492

4808

5132

5144

8025

7505

9000

8177

6310

5413

9000

6908

859

693

990

848

42(77371times

BSEC

TCP

-1)times

99HS-18

5595

4298

6295

5396

4729

4887

5031

4883

7366

6945

9000

7770

6312

4556

9000

6623

903

622

1125

883

4396AC

-93timesTo

go-II

5017

3977

6486

5160

5019

5195

5237

5150

5922

5486

9000

6803

4689

3484

9000

5724

1007

728

1167

967

4496AC

-93times99HS-18

5595

4812

6924

5777

5036

5385

5205

5209

7411

5378

9000

7263

5873

3979

9000

6284

1013

652

1334

1000

45To

go-IItimes

99HS-18

4670

4497

7293

5487

4718

4319

4796

4611

5688

5431

9000

6706

3980

3665

9000

5548

939

653

1462

1018

Mean

5043

4482

6271

5265

4792

498149

23

4899

6377

584190

00

7072

5079

4190

9000

6090

898

701

1168

922

SE097

106

161

mdash213

191

190

mdash19

818

7mdash

mdash14

914

6mdash

mdash036

047

068

mdashCD

272

296

453

mdash597

537

534

mdash555

525

mdashmdash

421

409

mdashmdash

103

133

192

mdashlowastTransfo

rmed

data

(b)

Sr

No

Genotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

908

785

2245

1313

859

1537

1924

1440

154

143

160

152

6267

4800

3833

4967

212

227

249

229

15644

15458

13267

1479

02

H7729-2

1125

1175

1789

1363

317

2400

2811

1843

093

191

252

179

6700

5333

5100

5711

185

210

234

210

18850

18565

15389

17601

3G73-107

1166

1475

1966

1536

943

1744

4945

2544

143

129

366

213

6700

5433

5300

5811

218

219

238

225

15009

1496

513503

14492

477245

1438

1373

2085

1632

607

1863

5957

2809

117

180

509

269

6100

4800

5100

5333

232

221

253

235

14036

13802

11742

1319

35

CVJ-2-5-3-1-3

1214

1437

1807

1486

1163

1610

4166

2313

171

141

407

240

6300

4933

4600

5278

239

227

270

245

1994

317261

1497

017391

61305

858

1137

1737

1244

540

967

4861

2123

125

088

417

210

6533

5033

4800

5456

244

213

257

238

15527

15405

12274

14402

8 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

777371timesBS

ECT

CP-1

1377

1487

1892

1585

928

2660

3330

2306

150

258

301

236

5733

4533

4700

4989

242

220

293

252

1513

814550

1315

014279

896AC

-93

1303

1259

1852

1471

1056

1445

5972

2825

185

147

586

306

5500

4833

3833

4722

256

304

318

293

15577

14032

10000

13203

9To

go-II

1056

1220

2272

1516

924

1466

9113

3834

146

129

883

386

6167

4533

4600

5100

185

149

124

153

14578

12073

10033

12228

1099HS-18

1277

1075

1808

1387

1241

2833

7070

3715

228

226

615

356

6100

5000

4167

5089

243

173

324

247

12627

10355

1017

311052

1H77833-2times

H7729-2

1432

1498

2233

1721

1350

4405

8373

4709

159

499

782

480

5767

4433

3800

4667309

290

248

282

21010

18071

15847

18309

2H77833-2times

G73-107

1675

1635

2205

1838

1236

3211

5789

3412

134

313

547

331

5833

4767

3833

4811

262

295

236

265

21253

20449

15808

1917

0

3H77833-2times

77245

1577

1522

2263

1787

1058

6933

8719

5570

255

553

782

530

5267

4633

4600

4833

273

201

265

246

2074

119511

15208

18487

4H77833-2times

CVJ-2-5-3-1-3

1791

1757

2165

1904

20218030

5276

5109

354

779

477

537

5533

4233

3767

4511

165

330

351

282

21827

20072

17110

19670

5H77833-2times1305

1700

1842

28412128

1427

6228

9134

5596

570

579

813

654

5800

4267

3333

4467340

426

451

406

19816

19444

16037

18432

6H77833-2times

(77371times

BSEC

TCP

-1)

1453

1515

3120

2029

789

465510428

5291

211

408

858

492

5233

4167

3267

4222

203

283

267

251

20677

1913

816470

1876

2

7H77833-2times

96AC

-93

1835

1783

2427

2015

2532

6649

1610

684

29

350

650

1473

824

5467

4233

3700

4467243

239

256

246

22278

20434

15735

19482

8H77833-2times

Togo-II

1564

15632500

1876

1882

4772

11660

6105

296

469

972

579

5667

4533

440048

67

169

260

211

213

2412

82375

619389

22424

9H77833-2times

99HS-18

159318002049

1814

1571

480214024

6799

203

544

1547

765

6033

460040

0048

78

261

225

256

247

23466

21081

1793

320

827

10H7729-2times

G73-107

14951568

2075

1713

959

5157

8517

4877

218

382

666

422

6133

5067

4833

5344

300

273

239

271

23651

2191

717083

20884

11H7729-2times77245

1557

1537

2613

1902

1851

5756

5729

4445366

562

524

484

5633

4800

4633

5022

133

233

297

221

1979

219914

15250

18319

12H7729-2times

CVJ-2-5-3-1-3

18442033

28932257

1651

806114885

8199

264

597

1054

638

6333

4600

4233

5056

170

211

217

199

24629

23339

1970

022556

13H7729-2times1305

1325

1677309920

34

1756

724314048

7682

318

635

1232

728

630046

67

3633

4867298

261

361

307

21666

19371

17422

19486

14H7729-2times(77371

timesBS

ECTCP

-1)

1958

1887

3105

2317

1118

4675

14411

6734

242

457

1318

672

5900

4500

3800

4733

164

209

240

204

23661

1995

419250

20955

15H7729-2times

96AC

-93

1512

1717

2779

2002266844

08

11393

6156

356

500

1211

689

5633

4533

4200

4789

221

195

282

232

2194

921635

17213

20266

Advances in Agriculture 9

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

16H7729-2times

Togo-II

1519

1738

2159

1805

2437

9867

1616

294

89

361

961

1527

950

6167

4700

4200

5022

234

272

230

246

26338

2414

520553

23678

17H7729-2times

99HS-18

1938

1787

3151

2292

1574

6218

11318

6370

290

606

956

617

6067

4767

406749

67

172

210

199

194

24689

21854

1778

921444

18G73-107times77245

1856

1813

2210

1960

2435

8550

7828

6271338

633

612

528

5500

4633

440048

44

199

255

307

254

1993

619277

16095

18436

19G73-107times

CVJ-2-5-3-1-3

1517

1769

2407

1897

1556

7728

9136

6140

239

876

917

677

5900

4300

3400

4533

233

266

315

272

23418

22333

19317

21689

20G73-107times1305

1217

1507

2832

1852

1777

5189

1114

960

38

289

588

1197

691

6733

5000

4233

5322

286

210

312

269

2078

81992

817067

19261

21G73-107times(77371

timesBS

ECTCP

-1)

18551946

2730

2177

1801

3482

10415

5233

239

277

753

423

5500

4467

3433

4467243

197

273

238

23817

20263

17425

20502

22G73-107times

96AC

-93

1718

1840

28612140

1345

9333

14669

8449

297

845

1278

807

5600

5033

4633

5089

270

243

300

271

28537

21461

16875

22291

23G73-107timesTo

go-II

168018083087

2192

1112

4678

9319

5036

186

473

985

548

6033

4767

4200

5000

210

209

231

217

24864

24609

21625

23699

24G73-107times99HS-18

1398

1675

3370

2148

2753

8672

11918

7781

362

691

981

678

566746

00

3500

4589

170

247

270

229

24677

20567

17855

21033

2577245times

CVJ-2-5-3-1-3

1500

1728

2203

1810

2282

4679

11492

6151

328

424

1116

623

6000

4333

420048

44300

231

370

300

21257

20497

1772

519826

2677245times1305

143815772080

1698

949

10439

12053

7814

121

915

992

676

5767

4800

3500

4689

168246

316

244

1978

91779

015517

17699

2777245times(77371times

BSEC

TCP

-1)

150516972846

2016

3222

8518

12621

8120

469

827

1141

812

5400

4367

3833

4533

196

225

242

221

2092

819485

1719

219202

2877245times96AC

-93

1653

1797

2117

1856

1934

6056

11543

6511

352

563

911

608

5333

4267

3833

4478

186

169

198

184

22003

19054

1676

719275

2977245timesTo

go-II

1723

1718

3163

2201

1749

8250

15443

8481229

806

1412

816

5967

4700

4067

4911

174

245

298

239

22075

23503

19875

21818

3077245times99HS-18

2016

1970

28542280

3217

13027

3531

6591395

965

285

549

5733

4467

3833

4678

148

228

209

195

2212

721462

1690

020

163

31CV

J-2-5-3-1-3times

1305

1358

1422

2179

1653

1254

2803

1019

44750

178

254

801

411

6033

4467

3367

4622

312

193

330

278

22269

19547

17250

19689

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

1335

1742

2400

1826

922

5226

12806

6318

236

458

1054

583

5633

4433

3600

4556

321

315

259

298

2210

419524

17087

19572

33CV

J-2-5-3-1-3times

96AC

-93

1552

16803260

2164

166710094

11941

7901250

974

1115

779

5533

4533

446748

44

170

202

317

230

2392

123317

19333

2219

0

34CV

J-2-5-3-1-3times

Togo-II

142216072638

1889

1012

4163

11061

5412

261

377

891

510

6167

4967

4533

5222

207

225

325

252

23590

22860

20278

22243

35CV

J-2-5-3-1-3times

99HS-18

1435

1600

2700

1912

3056

3237

7089

4461449

301

658

469

5700

44673800

4656

148

178

225

183

22444

20240

1696

719884

361305times(77371times

BSEC

TCP

-1)

1499

1767

2058

1774

1882

4665

7920

4822

356

426

724

502

566742673800

4578

271

242

305

273

22011

19295

16417

19241

371305times96AC

-93

16781605

2757

2014

1516

10037

11960

7838

311

11381284

911

560040

673400

4356

182

276

251

236

2396

519625

15800

1979

738

1305timesTo

go-II

1177

16802249

1702

902

4676

9388

4989

241

456

795

497

6533

5533

4733

5600

214

266

296

259

21894

20217

19222

20444

391305times99HS-18

1774

1767

2766

2102

2145

4628

10618

5797

301

447

991

580

5933

4500

3733

4722

128

142

180

150

2275

719413

16967

1971

2

40(77371times

BSEC

TCP

-1)times

96AC

-93

1748

1795

31712238

1537

12586

14379

9501

211

1104

1183

833

546744

00346744

44

127

201

226

185

23294

21089

17858

2074

7

10 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(

days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

41(77371times

BSEC

TCP

-1)times

Togo-II

19652007

2653

2208

2136

5601

9503

5747

315

635

1001

650

5533

4833

410048

22

213

241

286

247

22662

22094

17667

20808

42(77371times

BSEC

TCP

-1)times

99HS-18

15011687

2705

1964

1935

7253

11806

6998

314

704

1067

695

5467

4433

5133

5011

177

167

175

173

22305

19277

16392

19325

4396AC

-93timesTo

go-II1759

1618

2123

1834

1539

10347

12212

8033

325

907

1090

774

5900

450039674789

214

269

339

274

23252

21554

16875

20560

4496AC

-93times

99HS-18

1353

1618

2925

1966

3532

7223

11950

7569

440

654

1024

706

5600

4900

4533

5011

149

175

206

177

24494

20698

18111

2110

145

Togo-IItimes

99HS-18

1117

1278

2582

1659

3448

6764

11910

7374

477

767

1207

817

6000

5133

4333

5156

183

203

216

201

17683

15541

14333

15853

Mean

1513

1618

2492

1874

1656

5773

9854

5761

272

538

885

565

586846

534116

4879

218

233

268

240

21297

19547

16529

1912

4SE

101

073

098

mdash079

152

317

mdash014

014

028

mdash351

365

428

mdash036

039

046

mdash985

1173

1211

mdashCD

284

205

276

mdash221

427

890

mdash038

040

079

mdash12

513

015

2mdash

013

014

016

mdash351

418

431

mdash

(c)

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

1642

1667

1744

1684

1504

1349

1526

1460

2783

2405

2634

2607

836

745

908

830

4287

3754

4160

4067

2H7729-2

1693

1817

1950

1820

1012

859

1016

962

3433

2809

2591

2944

649

597

651

632

4445

3668

3607

3906

3G73-107

2000

2028

2077

2035

2280

1966

2312

2186

4695

4023

4229

4316

1281

1170

1463

1305

6975

5989

6541

6502

477245

1523

1630

1728

1627

1796

2489

2702

2329

5423

4851

4498

4924

748

1464

1743

1319

7219

7341

7200

7253

5CV

J-2-5-3-1-3

2282

2692

2787

2587

2411

2643

2901

2652

5857

4836

5024

5239

1206

1335

1769

1437

8268

7479

7925

7891

61305

1851

1951

2054

1952

2026

1763

1908

1899

4657

4414

4463

4511

1468

1336

1539

1448

6683

6177

6371

6410

777371timesBS

ECTCP

-11629

1645

1733

1669

1244

1692

1884

1607

4925

4183

4435

4515

625

1092

1483

1067

6169

5875

6319

6121

896AC

-93

1379

1519

1600

1499

2066

2942

3088

2698

4501

4095

4229

4275

984

1565

1678

1409

6567

7037

7316

6973

9To

go-II

1904

2057

2103

2021

2167

1844

2358

2123

4520

3446

3904

3957

1298

1138

1502

1313

6687

5290

6262

6080

1099HS-18

1475

1485

1633

1531

3180

2904

3382

3155

5633

4139

5205

4992

1656

1536

1879

1690

8813

7043

8587

8147

1H77833-2timesH7729-2

2010

2046

2133

2063

3645

3075

4377

3699

7675

6540

7199

7138

2014

1708

2880

2201

11320

9615

11576

10837

2H77833-2timesG73-107

2066

2101

2122

2096

5002

4053

4601

4552

7882

7430

6963

7425

3146

2565

2808

2840

12884

11482

11564

11977

3H77833-2times77245

1764

2048

2083

1965

3769

4497

4559

4275

8681

8515

8475

8557

2284

3356

3377

3006

12450

13012

13034

12832

4H77833-2times

CVJ-2-5-3-1-3

2205

2383

2733

2441

3693

5655

5220

4856

11656

10287

1010

510683

2198

4488

4176

3621

15348

1594

215325

15538

5H77833-2times1305

2209

2423

2491

2374

6008

5842

6933

6261

15238

14311

13889

14479

3758

4057

5591

4469

21246

2015

320822

2074

0

6H77833-2times(77371times

BSEC

TCP

-1)

1789

1823

1992

1868

3593

4288

4903

4262

8922

6522

7429

7625

2178

3224

3070

2824

12515

10811

12333

11886

7H77833-2times96AC

-93

1739

1960

2011

1903

4557

4350

4818

4575

7916

6068

7290

7092

2411

2529

3191

2710

12474

10419

1210

811667

8H77833-2timesTo

go-II

1927

2389

2406

2241

4185

5828

6215

5409

12319

10279

11023

11207

2433

4254

4631

3773

16504

1610

717238

16616

9H77833-2times99HS-18

1941

2245

2330

2172

4227

4139

4529

4298

9211

8120

7969

8433

3154

3184

3253

3197

13438

12259

12498

1273

210

H7729-2timesG73-107

1968

2109

2158

2079

3725

3042

3488

3418

7313

5956

6174

6481

2822

2001

2357

2393

11038

8998

9662

9899

Advances in Agriculture 11

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

11H7729-2times77245

1847

2048

2200

2032

2467

3759

5466

3898

7672

7449

8921

8014

1356

2350

3796

2500

1013

911208

14387

11911

12H7729-2times

CVJ-2-5-3-1-3

2218

2585

2767

2523

3674

4372

4483

4176

10053

9467

8664

9395

2417

3312

3345

3025

1372

813839

1314

713571

13H7729-2times1305

2169

2338

2547

2352

6728

6166

6792

6562

13333

11662

13301

1276

54128

4501

5660

4763

20061

17828

20093

19327

14H7729-2times(77371times

BSEC

TCP

-1)

1925

2095

2127

2049

3530

4165

4649

4115

9638

6917

7698

8084

1681

2833

3150

2555

1316

8110

82

12347

1219

915

H7729-2times96AC

-93

2012

2070

2180

2087

4955

4447

4596

4666

8738

6882

7762

7794

3754

3558

3181

3498

13693

11329

12359

12460

16H7729-2timesTo

go-II

2138

2428

2563

2377

4949

4660

5118

4909

8549

7243

7191

7661

3586

3353

3028

3322

13498

11903

12310

12570

17H7729-2times99HS-18

1899

1933

2073

1969

4046

4527

4988

4520

8492

7895

8144

8177

2561

3378

3553

3164

12539

12422

1313

212698

18G73-107times77245

1840

2253

2437

2177

2580

4776

5915

4424

11673

10608

10459

10913

1358

3163

4286

2936

14253

15385

16374

15337

19G73-107times

CVJ-2-5-3-1-3

2324

2537

2691

2517

4958

5030

5599

5196

9524

9395

1114

610022

3241

3645

4444

3776

14483

14425

1674

515217

20G73-107times1305

2228

2433

2583

2415

6340

5794

6373

6169

12490

1015

8119

61

11536

4199

4199

5018

4472

18830

1595

218334

1770

6

21G73-107times(77371times

BSEC

TCP

-1)

1943

2128

2220

2097

3936

3980

5770

4562

11697

9858

10303

10619

1874

2369

4181

2808

15633

13839

16073

1518

222

G73-107times96AC

-93

2118

2218

2317

2217

4270

5843

6201

5438

10319

9872

1015

910117

2703

4460

4379

3847

14589

15715

16359

15555

23G73-107timesTo

go-II

2237

2365

2733

2445

5736

5175

6822

5911

12098

10808

1299

611967

3984

3670

5372

4342

17835

1598

219818

17878

24G73-107times99HS-18

2025

2081

2322

2143

3558

4229

6612

4800

12350

9685

11516

1118

42196

2782

5009

3329

1590

813914

1812

915983

2577245timesCV

J-2-5-3-1-3

2109

2360

2675

2381

6760

6558

7299

6872

15225

11861

1416

21374

94225

4752

5839

4939

2198

518418

21461

20621

2677245times1305

1919

2100

2197

2072

5700

5142

5347

5397

11687

10565

10737

10996

4318

3956

4244

4173

17386

1570

816085

16393

2777245times(77371times

BSEC

TCP

-1)

1851

1935

2020

1936

2577

2460

2838

2625

6989

4778

4636

5468

1631

1662

1999

1764

9566

7238

7474

8093

2877245times96AC

-93

1729

1780

2133

1881

5541

5058

5748

5449

11952

8174

9526

9884

3646

3587

4071

3768

17493

13232

15274

15333

2977245timesTo

go-II

2300

2508

2658

2489

3067

5431

5963

4820

11737

11021

1216

411641

1598

4053

4770

3474

14805

16452

1812

616461

3077245times99HS-18

1982

2019

2223

2075

3846

3119

4225

3730

7493

6720

6476

6896

3205

2578

2643

2809

11339

9839

1070

110626

12 Advances in Agriculture

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

31CV

J-2-5-3-1-3times1305

2406

2553

3067

2675

4178

4796

6281

5085

13822

11425

12252

12500

2472

3241

4907

3540

18000

16222

18532

17585

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

2142

2173

2238

2184

4522

5235

5640

5132

11673

9658

10248

10526

2614

3766

4178

3519

1619

514893

15888

15659

33CV

J-2-5-3-1-3times

96AC

-93

2350

2477

2600

2476

4676

4280

6090

5015

11975

9832

10868

10891

3097

2931

4445

3491

16651

14111

1695

815907

34CV

J-2-5-3-1-3times

Togo-II

2093

2666

2989

2583

4058

3727

5190

4325

12588

10717

11217

11507

2742

2570

4185

3166

16645

14443

16407

15832

35CV

J-2-5-3-1-3times

99HS-18

2028

2311

2386

2242

3287

2513

5356

3719

10055

9733

1036

410051

2004

1581

4251

2612

13342

12246

1572

01376

9

361305times(77371times

BSEC

TCP

-1)

2005

2173

2210

2129

3569

4692

5140

4467

11652

9826

10041

10507

2005

3665

4179

3283

15221

14518

1518

214974

371305times96AC

-93

1822

2003

2047

1957

5115

4023

4813

4650

9965

7675

7571

8404

3456

2647

3155

3086

15080

11698

12384

13054

381305timesTo

go-II

2545

2583

2644

2591

6885

7283

6795

6987

12265

11410

12583

12086

4500

6120

5350

5323

1915

018693

19378

19074

391305times99HS-18

2070

2408

2593

2357

5219

4332

4800

4784

1092

97677

8968

9192

3924

3332

3840

3699

1614

912009

1376

913976

40(77371times

BSEC

TCP

-1)

times96AC

-93

2056

2115

2157

2109

3158

4463

4717

4113

9166

7540

8100

8268

1880

3542

3347

2923

12324

12003

12817

12381

41(77371times

BSEC

TCP

-1)

timesTo

go-II

1868

2253

2450

2190

4422

6458

7038

5973

1415

312369

1379

713439

2291

4646

5586

4174

18575

18827

20835

19412

42(77371times

BSEC

TCP

-1)

times99HS-18

1995

2026

2209

2077

2720

2909

3543

3057

7392

4692

5023

5702

1766

2187

2147

2033

10112

7601

8566

8760

4396AC

-93timesTo

go-II

1953

2027

2511

2164

5972

4391

7126

5830

15537

1392

714432

14632

3780

2538

5655

3991

21510

18318

21558

20462

4496AC

-93times99HS-18

1952

2021

2242

2072

3465

2758

4496

3573

5959

5186

6094

5746

2707

2154

2409

2423

9424

7944

10590

9319

45To

go-IItimes

99HS-18

1932

2008

2658

2199

5381

4995

5436

5270

10299

8876

9244

9473

3986

3842

3668

3832

15680

13871

14680

1474

3Mean

1982

2146

2301

2143

3963

4123

4836

4307

9498

8087

8663

8749

2510

2914

3513

2979

13460

12210

13499

13056

SE243

198

245

mdash671

501

626

mdash959

750

822

mdash423

349

344

mdash1155

927

1167

mdashCD

087

071

087

mdash239

178

223

mdash342

267

293

mdash15

112

412

2mdash

411

330

416

mdash

Advances in Agriculture 13Ta

ble4Analysis

ofvaria

ncefor

10times10

half-diallelfor

different

charactersin

threee

nviro

nments

(a)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Germination

()

Rateof

emergence

STI()

SSTI

()

Num

bero

fleavesseedling

Seedlin

gheight

(cm)

Freshweight

(g)seedlin

gDry

weight

(g)seedlin

g

E 1

Replicates

2652731

596768

783393

20700

93018674

33764

1033999

002590

Treatm

ents

541097

0255lowastlowast

8181153lowastlowast

30863092lowastlowast

20680900lowastlowast

1100708lowastlowast

2106317lowastlowast

930829527lowastlowast

319767lowastlowast

Parents

95118

163lowastlowast

14289925lowastlowast

59114816lowastlowast

18880254lowastlowast

1084227lowastlowast

1087906lowastlowast

22561719lowastlowast

042162lowastlowast

Hybrid

s44

11423573lowastlowast

3398933lowastlowast

25697199lowastlowast

20633716lowastlowast

472874lowastlowast

1391526lowastlowast

674123518lowastlowast

261839lowastlowast

Parentsv

ersush

ybrid

s1

43693104lowastlowast

163619

916lowastlowast

3896838

3896

2793lowastlowast

28873741lowastlowast

4272

2798lowastlowast

234144289lowastlowast

5367022lowastlowast

Error

108

281184

1356832

1173684

674337

040104

306

462

2015

1619

005535

E 2

Replicates

2583819

3707389lowast

1596635

198738

034925

448213

899529

007177

Treatm

ents

549997443lowastlowast

2825177lowastlowast

35378177lowastlowast

19074306lowastlowast

665284lowastlowast

1764

081lowastlowast

257694570lowastlowast

2177519lowastlowast

Parents

96507784lowastlowast

3718

879lowastlowast

69866289lowastlowast

1798

5404lowastlowast

705379lowastlowast

1408518lowastlowast

10612519lowastlowast

077429lowastlowast

Hybrid

s44

10569731lowastlowast

2127302lowastlowast

2910

2537lowastlowast

19429240lowastlowast

329256lowastlowast

7000

03lowastlowast

186026552lowastlowast

1483428lowastlowast

Parentsv

ersush

ybrid

s1

16223710lowastlowast

25488361lowastlowast

1113

326

13257362lowastlowast

15089673lowastlowast

5178

3569lowastlowast

5634825791lowastlowast

51618348lowastlowast

Error

108

334760

1097039

1049505

635570

06764

116

0576

694613

006206

E 3

Replicates

2894398

4808815lowast

mdashmdash

051076

095989

3768421

030220

Treatm

ents

541261560

0lowastlowast

4842216lowastlowast

mdashmdash

2811077lowastlowast

5947316lowastlowast

3870844

50lowastlowast

3445076lowastlowast

Parents

97135

735lowastlowast

1097

6388lowastlowast

mdashmdash

1468699lowastlowast

1112

764lowastlowast

136081650lowastlowast

1307836lowastlowast

Hybrid

s44

13217740lowastlowast

1379360

mdashmdash

15540

71lowastlowast

4581632lowastlowast

252084767lowastlowast

2378032lowastlowast

Parentsv

ersush

ybrid

s1

35440

180lowastlowast

102000335lowastlowast

mdashmdash

70200767lowastlowast

109548362lowastlowast

8586095705lowastlowast

69630188lowastlowast

Error

108

782907

1085444

mdashmdash

139771

291325

3019

169

02400

4(b)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Panicle

emergence

Effectiv

etillersplant

Planth

eight

Earlength

Earw

eightp

lant

Dry

fodd

eryieldplant

Grain

yieldplant

Totalbiological

yieldplant

E 1

Replicates

231860

61lowastlowast

416348lowastlowast

1672

9472lowast

1706

411077192

9634539

392777

115544

59Treatm

ents

544152

368lowastlowast

085657lowastlowast

326706089lowastlowast

1602572lowastlowast

6177

0406lowastlowast

304113

204lowastlowast

34285030lowastlowast

575617

582lowastlowast

Parents

94511481lowastlowast

018538lowastlowast

140807476lowastlowast

2240093lowastlowast

11653

959lowastlowast

27014155lowastlowast

3858650lowastlowast

61321919lowastlowast

Hybrid

s44

3201347lowastlowast

100797lowastlowast

110395657lowastlowast

1010

497lowastlowast

40296671lowastlowast

171271411lowastlowast

2413

6880lowastlowast

302999650lowastlowast

Parentsv

ersush

ybrid

s1

4276

5286lowastlowast

02360

9lowast11517452

616lowastlowast

2191

6167lowastlowast

1457662749lowastlowast

8643043524lowastlowast

7546

41053lowastlowast

17199467546lowastlowast

Error

108

468159

004907

3692099

224610

17114

303498126

682350

5075268

E 2

Replicates

2656364

002556

61575565lowastlowast

828842lowastlowast

589094

2268506

260877

1507116

Treatm

ents

542999618lowastlowast

069822lowastlowast

268998723lowastlowast

2436886lowastlowast

60592784lowastlowast

247259877lowastlowast

4192

0073lowastlowast

513483489lowastlowast

Parents

926300

00lowastlowast

048079lowastlowast

165555

405lowastlowast

3871874lowastlowast

1412

2393lowastlowast

19543682lowastlowast

3132

426lowastlowast

57230706lowastlowast

Hybrid

s44

2533

165lowastlowast

07344

9lowastlowast

9616

2857lowastlowast

1463359lowastlowast

35495366lowastlowast

154759288lowastlowast

26262465lowastlowast

293492304lowastlowast

Parentsv

ersush

ybrid

s1

26850135lowastlowast

105929lowastlowast

880476

6697lowastlowast

32357161lowastlowast

1583112746lowastlowast

6366731560lowastlowast

107994

3646lowastlowast

14299370699lowastlowast

Error

108

507598

005829

5234122

149406

955133

2142411

462311

3273886

E 3

Replicates

2029697

021161

9459847

143746

985141

46800

6492

6334

3345160

Treatm

ents

548045499lowastlowast

094511lowastlowast

192420108lowastlowast

3279225lowastlowast

72049211lowastlowast

298953

021lowastlowast

55061308lowastlowast

651732994lowastlowast

Parents

98010

741lowastlowast

094166lowastlowast

116037343lowastlowast

3702185lowastlowast

16305190lowastlowast

2323644

0lowastlowast

4553

893lowastlowast

71484982lowastlowast

Hybrid

s44

6254377lowastlowast

095514lowastlowast

7378

1513lowastlowast

2188348lowastlowast

31813193lowastlowast

190263916lowastlowast

31572179lowastlowast

368689934lowastlowast

Parentsv

ersush

ybrid

s1

8716

7710lowastlowast

053496lowast

609996

3199lowastlowast

47471212lowastlowast

2344

130203lowastlowast

756272

2861lowastlowast

154314

9707lowastlowast

18327859

719lowastlowast

Error

108

696981

007883

5578505

228608

1491014

2574

223

449588

5187705

lowast

Sign

ificant

at119875=005

lowastlowastSign

ificant

at119875=001

14 Advances in Agriculture

Table 5 Mean performance of the genotypes for membrane thermostability in two environments

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594

Advances in Agriculture 15

Table 5 Continued

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434

Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash

lowastValues are transformed values

0

6

12

18

24

30

36

42

48

54

minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity

G73-10796AC-93CVJ-2-5-3-1-3

Togo-II99HS-18

H77833-2H7729-2772451305

H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305

1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305

H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305

77245 times 1305

77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305

77371 times BSECT CP-1

H77833-2 times G73-107

Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits

moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling

survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The

16 Advances in Agriculture

Table6Po

oled

correla

tioncoeffi

cientamon

gvario

uscharactersin

threee

nviro

nments

Characters

Germination

Rateof

emergence

STI

SSTI

Leaves

seedlin

gSeedlin

gheight

Fresh

weight

Dry

weight

MTS

Panicle

emergence

Effectiv

etillersplant

Plant

height

Earlength

Ear

weightp

lant

Dry

fodd

eryield

plant

Grain

yield

plant

Total

biological

yield

plant

Germination

1000

0357lowastlowast

0353lowastlowast

0590lowastlowast

0230

0373lowastlowast

0293lowast

0260

0276lowastminus0135minus0478lowastlowast

0326lowast

minus0069minus0104

minus0190

minus0145

minus0167

Rateof

emergence

1000

0233

0351lowastlowast

0605lowastlowast

0585lowastlowast

0551lowastlowast

0524lowastlowast

0139minus0593lowastlowast

0032

0517lowastlowast

0217

0388lowastlowast

0361lowastlowast

0348lowastlowast

0377lowastlowast

STI

1000

0837lowastlowast

0241

0129

0081

004

80890lowastlowast

minus0215

minus0211

0030minus0007minus0043

minus0101

minus0111

minus0084

SSTI

1000

0233

0264

0183

0140

0745lowastlowast

minus0247minus0268lowast

0184minus0017minus006

6minus0129

minus0144

minus0112

Num

bero

fleaves

1000

0683lowastlowast

0633lowastlowast

060

4lowastlowast

0176minus0474lowastlowast

0109

0576lowastlowast

0496lowastlowast

064

1lowastlowast

0607lowastlowast

0630lowastlowast

0630lowastlowast

Seedlin

gheight

1000

0703lowastlowast

0663lowastlowast

0031minus0493lowastlowast

minus0115

0735lowastlowast

0360lowastlowast

0526lowastlowast

0520lowastlowast

0515lowastlowast

0532lowastlowast

Freshweight

1000

0957lowastlowast

minus0016

0425lowastlowast

minus0082

0661lowastlowast

0351lowastlowast

0581lowastlowast

0496lowastlowast

0576lowastlowast

0533lowastlowast

Dry

weight

1000minus0033minus0428lowastlowast

minus0016

0634lowastlowast

0354lowastlowast

0620lowastlowast

0509lowastlowast

0614lowastlowast

0554lowastlowast

MTS

1000minus040

minus0177minus0037

0015

minus0074

minus0138

minus0131

minus0120

Panicle

emergence

1000

minus0150minus0269lowast

0022minus0329lowast

minus0337lowast

minus0280lowast

minus0341lowast

Effectiv

etillersplant

1000

minus0012

0170

0366lowastlowast

040

5lowastlowast

0323lowast

0401lowastlowast

Planth

eight

1000

0650lowastlowast

0603lowastlowast

0596lowastlowast

0619lowastlowast

0610lowastlowast

Earlength

1000

0608lowastlowast

0671lowastlowast

064

8lowastlowast

066

4lowastlowast

Earw

eightp

lant

1000

0910lowastlowast

0984lowastlowast

0957lowastlowast

Dry

fodd

eryield

plant

1000

0907lowastlowast

0991lowastlowast

Grain

yield

plant

1000

0950lowastlowast

Totalbiological

yield

plant

1000

lowastSign

ificant

at119875=005lowastlowastSign

ificant

at119875=001

Advances in Agriculture 17

faster germination and early vigour in seedling could act asphysical indicators for heat tolerance

Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits

Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics

Conflict of Interests

The authors declare that there is no conflict of interestsregarding the publication of this paper

References

[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981

[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993

[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006

[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993

[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013

[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007

[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965

[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011

[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001

[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009

[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962

[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967

[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963

[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001

Submit your manuscripts athttpwwwhindawicom

Nutrition and Metabolism

Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Food ScienceInternational Journal of

Agronomy

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Microbiology

The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom

Applied ampEnvironmentalSoil Science

Volume 2014

AgricultureAdvances in

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

BiodiversityInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

GenomicsInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Plant GenomicsInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Biotechnology Research International

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Forestry ResearchInternational Journal of

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Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

EcologyInternational Journal of

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Cell BiologyInternational Journal of

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Page 4: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

4 Advances in Agriculture

heat stress were noted in each genotype on daily basis Obser-vations continued till the surviving seedlingswere established(15 DAE) and there was no further mortality Moreover thesoil moisture was depleting near to the permanent wiltingpoint and drought stress could also come into play along withheat stress to further continue the experiment Since experi-ment proceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedlingsurvival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of heat stress at seedling stage was observed on latergrowth stages

231 Thermotolerance Characteristics For heat tolerancecharacteristics 3 indices namely seedling thermotoleranceindex (STI) [4] seed to seedling thermotolerance index(SSTI) [8] and membrane thermostability (MTS) wereapplied to evaluate the hybrids and parents STI was cal-culated as the ratio of number of seedlings survived to thenumber of seedling emergences expressed in percentageSeed to seedling thermotolerance index (SSTI) was calculatedas the ratio of seedling survival to the number of seedlingsexpected to emerge expressed in percentage [8] The SSTIis an extension of STI by taking expected germination intoaccount It was necessary to correct the effect of under soilmortality (USM) The loss of germination due to heat (ieunder soil mortality) can be measured only after a standardgermination test Therefore the germination of seed fromthe same lot under monsoon environment (main pearl milletgrowing season where no under soil mortality occurreddue to heat stress) was taken as expected germination forcalculation of SSTI

232 Membrane Thermostability These genotypes were alsoscreened through laboratory technique (membrane ther-mostability) [9 10] The young leaves of 21-day-old seedlingsof each genotype in three replication plots were used formembrane thermostability test A middle portion of 10 cmfrom each leaf was taken and the midrib was removed andtwo equal halves of the 10 cm each were made Each halfwas taken to provide replications Each leaf portion was cutinto pieces and thoroughly washed and rinsed with distilledwater two times to remove the electrolytes adhering to theplant tissue as well as electrolyte released from the cutting ofplant tissue and put them into test tubes After final rinsingtubes were drained maintaining sufficient water to preventdesiccation of plant material during heat treatment Thesetest tubes were covered with aluminium foil and incubated inwater bath at 55∘C for 15 minutes for giving heat treatmentAfter the heat treatment period 10mL of distilled waterwas added to the test tubes Then all the test tubes wereheld at 10∘C overnight to allow diffusion of electrolytes fromplant material Test tubes were brought out of refrigeratorand shaken well to mix the contents An initial electricalconductivity (EC) of both replications of each treatment (119879

1)

was determined with electrical conductivity meter Thenthese test tubes were autoclaved at 15 psi for 2 minutes forcomplete electrolyte leakage from the leaf samples These testtubes were then cooled at room temperature and contentsmixed and final EC was taken for all the treatments (119879

2)

Membrane thermostability (MTS) was calculated accordingto Ibrahim and Quick [9] and Yadav et al [10] as follows

Membrane Thermostability (MTS) = (1 minus1198791

1198792

) times 100

(2)

where 1198791is conductivity reading after heat treatment and 119879

2

is conductivity reading after autoclavingCompletely randomized design (CRD) for membrane

thermostabilitywas conducted to detect variations among thegenotypes

233 Morphological Characteristics The seedling growthcharacteristics namely germination () emergence rate(ER) number of leavesseedling (two-week stage) seedlingheight (two-week stage) seedling fresh weight (g) (four-week stage) and seedling dry weight (g) (four-week stage)were recorded The data for seedling height and number ofleaves per seedling was recorded on 10 random seedlings perplot Emergence rate (an index for speed of germination)can be used as a tool in pearl millet breeding programs forevaluation of seedling vigour Speed of germination is oneof the oldest concepts of seedling vigour The emergencerate (ER) was calculated by dividing the number of normalseedlings obtained at each counting by the number of daysseeds had been in the seed bed (DAS)The values obtained ateach count were then summed at the end of the germinationtest to obtain the emergence rate [11]

Emergence Rate (ER) =Seedling count

Days to first count+ sdot sdot sdot

+

Seedling countDays to final count

(3)

After termination of the heat stress on the seedlings byirrigating the field the experiment was continued to studythe effect of seedling heat stress on maturity traits on thesurvived plants The maturity traits observed were panicleemergence effective tillersplant plant height ear length earweightplant dry fodder yieldplant grain yieldplant andtotal biological yieldplant

24 Statistical Analysis Analysis of variance for randomizedblock design (RBD) for field traits that is germinationemergence rate number of leavesseedling seedling heightseedling fresh weight and seedling dry weight and com-pletely randomized design (CRD) for membrane thermosta-bility test was conducted under each environment on meanbasis [12] Analyses were done using the software OPSTATdeveloped at CCS HAU Hisar Correlations among the vari-ous characters studied were calculated to establish the natureand magnitude of associations among the traits [8 13] with

Advances in Agriculture 5

the software SPSS (SPSS Inc) Multivariate clustering wasdone based on paired group cluster method using similaritycoefficient under Past 140 software [14] Multivariate analysisbased on the Euclidean distancematrix was estimated amonggenotypes to distinguish genotypes from each other Thegrouping of genotypes was generated using paired groupcluster method

3 Results and Discussion

The excessive heat (soil temperature up to 630∘C in E2)

about 18∘C higher than the normal atmospheric temperature(Figures 1(a) and 1(b)) was responsible for mortality ofseedlings Many seedlings were found desiccated at the pointof contact besides the desiccation at the tipsThe temperaturearound the tip level (5 cm above the soil surface) also reachedup to 50∘C (Figure 1(c)) It could therefore be concluded thatthe seedlings had to survive in a really high temperature Themoisture level remained above the permanent wilting point(Figure 1(d)) therefore drought effect was not in the playThe heat tolerance was measured as STI (Table 3) throughthe field screening technique of Peacock et al [4] Anotherindex known as SSTI was also calculated as an improvementand extensionThe analysis of variance (RBD) for 8 charactersin 3 environments showed significant genotypic differencesNo variability was observed for STI and SSTI in E

3only In

this environment no seedling mortality was observed andthe germination was taken as standard The values for STIand SSTI were therefore equal and the highest for all thegenotypes in this environment and hence no variation wasobserved

The hybrids were evaluated on the basis of mean perfor-mance for various characters in each environment (Table 3)On the mean basis STI value ranged from 5332 to 8224with an average of 7072 The hybrid H7729-2 times CVJ-2-5-3-1-3 was the best scorer for heat tolerance followed byH77833-2 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II The hybrid H77833-2 times 1305 was found susceptible toheat stress Seed to seedling thermotolerance index (SSTI)which is expected to be more rigorous index deviated thevalues from STI according to variation in germination undernormal environment (E

3) as the SSTI also coversincludes

the under surface mortality (USM) during germinationThe reductions in values were not strictly proportional tothe STI The reduction is dependent on thermosensitivityof germination We also came across such hybrids whichshowed poor germination and high STI SSTI values rangedfrom 4605 to 7287 with an average of 6090 The genotypeH77833-2 times 96AC-93 had the highest worth for SSTI onmean basis followed byG73-107times (77371timesBSECTCP-1) andH77833-2 times (77371 times BSECT CP-1) (Table 3) The analysisof variance for ten different characters in three differentenvironments (two stressed and one normal) was carried outand the results showed significant genotypic differences forall the characters (Table 4)

A caution is needed for evaluating such genotypes underSTI The SSTI gave a highly reduced value putting thesecrosses in the susceptible list It may be concluded therefore

that SSTI is more dependable and desirable tool for screeningand evaluating the genotypes for heat tolerance The germi-nation was lower in E

2than in E

1 This period has the highest

range of temperature with a high average Genotype-wise thehighest germination (6209) was recorded for the genotypeH7729-2 times (77371 times BSECT CP-1) on mean basis whichgives a preliminary indication of resistance to heat while thegenotype 1times 6 had the lowest germination (3533)The hybridH77833-2 times G73-107 was adjudged as the fastest emergenceof seedlingsThe genotype (77371timesBSECTCP-1)times 96AC-93had the highest fresh weightseedlingThe genotype H7729-2 times Togo-II had the highest seedling dry weight E

1had lower

dry weightseedling than E2indicating the more heat stress

during the E1

31 Membrane Thermostability Membrane thermostability(MTS) has long been recognised as a parameter of heattolerance All the hybrids were put to membrane thermosta-bility test in the laboratory under standard protocol Acompletely randomised design (CRD) analysis was carriedout for membrane thermostability (MTS) The mean sum ofsquares due to genotypes was highly significant indicatingthat enough genetic variability was present for membranethermostability (MTS) in each environment which justifiedfurther analysis of the data The range of values lay between5073 and 7742 Among the parents H77833-2 G73-107CVJ-2-5-3-1-3 77371 times BSECT CP-1 96AC-93 Togo-II and99HS-18 were verified as resistant genotypes and H7729-2 77245 and 1305 genotypes were found susceptible asmentioned in Table 1 (on the basis of previous experiment)Among all the hybrids H7729-2 times CVJ-2-5-3-1-3 CVJ-2-5-3-1-3 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II werethree highly tolerant genotypes on the basis of membranethermostability (Table 5)

32 Correlations The pooled correlations among the charac-ters were calculated (Table 6) around heat tolerance indices(STI SSTI and membrane thermostability) with a view tofind whether there was any association of these characterswith seedling as well as maturity traits It was observedthat STI and SSTI recorded the highest significant cor-relation among themselves as they are related indices forheat tolerance Membrane thermostability also showed highsignificant correlation with STI as well as SSTI STI hada significant positive association with germination of seedonly among seedling and maturity traits studied SSTI inaddition was also positively correlated with germination andemergence rate and negatively associated with number ofeffective tillersplantMembrane thermostability also showedsignificant positive correlation with seed germination amongseedling and maturity traits studied While evaluating thediallel progenies it was also seen that the heat toleranceindices STI SSTI and membrane thermostability were notshowing any perceptible correlation with the rest of thedevelopmental traits (seedling andmaturity traits)The stressperiod was terminated by irrigating the field experiment after15 days fromdate of sowingwhen the seedlings get establishedand further mortality stopped due to heat Moreover the soil

6 Advances in Agriculture

Table3Perfo

rmance

ofthep

arentsandthec

rosses

ford

ifferentcharacters

(a)

Sr

No

Genotypes

Germination(

)Ra

teof

emergencelowast

STIlowast

()

SSTIlowast(

)Num

bero

fleavesseedlin

gE 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

3941

3737

6164

4614

4037

4109

4279

4142

7830

7387

9000

8072

4498

4133

9000

5877

661

502

800

654

2H7729-2

5513

4726

6092

5443

2815

4980

3258

3684

4835

4495

9000

6110

4441

3602

9000

5681

363

294

427

361

3G73-107

5037

4826

5918

5260

4534

4132

4481

4382

6187

5413

9000

6866

5213

4460

9000

6224

878

710

935

841

477245

4747

4624

5565

4978

3625

4604

4047

4092

4385

3580

9000

5655

3803

3040

9000

5281

377

292

437

369

5CV

J-2-5-3-1-3

4824

4832

6897

5518

5044

4907

4960

4970

7193

6813

9000

7669

4959

4785

9000

6248

802

580

923

768

61305

4326

3891

5404

4540

3376

5056

3573

4002

3903

3281

9000

5395

3211

2452

9000

4888

350

284

414

349

777371timesBS

ECTCP

-14747

4502

6320

5190

4934

5075

5065

5025

6952

6706

9000

7552

5056

4671

9000

6242

621

542

700

621

896AC

-93

4651

3818

5722

4730

4312

4915

4753

4660

6660

6001

9000

7220

5212

3920

9000

6044

701

590

899

730

9To

go-II

4613

3937

5276

4609

4245

4737

4797

4593

7198

6861

9000

7686

5980

4767

9000

6582

681

629

853

721

1099HS-18

4575

3823

6247

4882

4317

4659

4746

4574

7594

7319

9000

7971

5154

4172

9000

6109

745

554

915

738

1H77833-2timesH7729-2

5787

5295

6733

5938

4964

5015

4989

4989

6803

6504

9000

7436

5808

5134

9000

6647

711

585

780

692

2H77833-2timesG73-107

5472

4417

6405

5431

5448

5422

5239

5370

7231

6769

9000

7667

5980

4556

9000

6512

1101

705

1340

1049

3H77833-2times77245

4076

3707

5504

4429

5217

5446

5238

5300

6627

6224

9000

7284

4690

4037

9000

5909

740

525

965

744

4H77833-2timesCV

J-2-5-3-1-3

4843

4300

5856

5000

4862

4956

4913

4911

7061

6519

9000

7527

5575

4632

9000

6403

998

885

1258

1047

5H77833-2times1305

3296

2780

4521

3533

5076

4907

5238

5074

3732

3265

9000

5332

2765

2050

9000

4605

1192

934

1600

1242

6H77833-2times(77371times

BSEC

TCP

-1)5636

5539

6258

5811

4489

5219

5015

4908

6981

6482

9000

7488

6146

5680

9000

6942

898

726

1525

1050

7H77833-2times96AC

-93

5720

5515

6335

5857

4800

5186

5075

5021

7833

7786

9000

8206

6603

6256

9000

7287

1107

873

1193

1058

8H77833-2timesTo

go-II

4862

3453

5709

4675

5036

4829

4911

4925

6190

5500

9000

6897

5214

3339

9000

5851

979

670

1107

919

9H77833-2times99HS-18

4575

4418

5447

4813

5041

5074

5006

5040

5800

5073

9000

6625

4846

4133

9000

5993

978

733

1135

949

10H7729-2timesG73-107

5615

4962

6273

5617

5023

5535

5165

5241

6143

5501

9000

6881

5540

4441

9000

6327

1000

720

1363

1028

11H7729-2times77245

5472

5164

6281

5639

4745

5031

5028

4935

5572

5175

9000

6582

4901

4345

9000

6082

880

620

897

799

12H7729-2timesCV

J-2-5-3-1-3

5494

5124

6536

5718

4804

5316

4957

5026

7596

8076

9000

8224

6093

5680

9000

6924

981

932

1451

1121

13H7729-2times1305

3804

3826

5511

4380

3596

4724

4387

4236

6362

7075

9000

7479

4230

4517

9000

5916

926

725

1497

1049

14H7729-2times(77371times

BSEC

TCP

-1)

6369

4847

7412

6209

4847

5351

5246

5148

6377

6031

9000

7136

5683

4230

9000

6304

977

671

1380

1009

15H7729-2times96AC

-93

5314

4657

6702

5558

4514

5295

5102

4971

5612

4856

9000

6489

4613

3603

9000

5739

846

679

1397

974

16H7729-2timesTo

go-II

5827

5594

6651

6024

5072

5279

5271

5207

6563

5371

9000

6978

5763

4652

9000

6472

873

719

1040

877

17H7729-2times99HS-18

5741

4638

7918

6099

4847

5024

5021

4964

6179

5603

9000

6927

4882

3725

9000

5869

1012

730

1600

1114

18G73-107times77245

5115

4646

6103

5288

4977

5242

5450

5223

7006

6063

9000

7356

5685

4594

9000

6426

1021

790

1292

1034

19G73-107timesCV

J-2-5-3-1-3

5134

4894

6427

5485

4901

5317

5181

5133

6637

6124

9000

7254

5253

4710

9000

6321

1039

863

1254

1052

20G73-107times1305

4881

4561

7597

5680

4717

4791

4775

4761

6704

6380

9000

7361

4556

4115

9000

5890

1140

882

1657

1226

21G73-107times(77371times

BSEC

TCP

-1)

5636

5519

6283

5812

4986

5219

5203

5136

7990

6203

9000

7731

6620

5436

9000

7019

977

703

1395

1025

22G73-107times96AC

-93

5740

4510

6415

5555

5129

4982

5195

5102

6978

5556

9000

7178

6156

4019

9000

6392

1077

860

1264

1067

23G73-107timesTo

go-II

4460

4357

6044

4953

4396

5138

4951

4829

5279

4835

9000

6371

4018

3603

9000

5540

906

881

1189

992

24G73-107times99HS-18

5473

4356

6374

5401

5102

4955

5055

5037

6460

5197

9000

6886

5534

3704

9000

6079

1171

766

1635

1191

2577245timesCV

J-2-5-3-1-3

4766

4666

5921

5117

5170

4918

4900

4996

5928

5859

9000

6929

4785

4613

9000

6133

984

920

1194

1033

2677245times1305

4575

4060

6052

4895

5128

5297

5203

5210

5102

4990

9000

6364

3979

3464

9000

5481

1279

823

1550

1217

2777245times(77371times

BSEC

TCP

-1)

5017

4929

6022

5322

5108

5473

5274

5285

5308

5044

9000

6451

4498

4191

9000

5896

833

588

939

787

Advances in Agriculture 7

(a)Con

tinued

Sr

No

Genotypes

Germination(

)Ra

teof

emergencelowast

STIlowast

()

SSTIlowast(

)Num

bero

fleavesseedlin

gE 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

2877245times96AC

-93

5472

4705

622154

66

5135

5197

5006

5113

5247

4843

9000

6363

4708

3784

9000

5831

795

662

1058

838

2977245timesTo

go-II

5252

4492

5889

5211

5188

4861

5074

5041

4885

4263

9000

6049

4421

3379

9000

5600

977

751

1243

990

3077245times99HS-18

5740

4099

6872

5570

5249

4898

5258

5135

5628

4616

9000

6415

4863

3014

9000

5626

1045

863

1184

1031

31CV

J-2-5-3-1-3times1305

4364

3944

5700

4669

4974

4517

4650

4714

6097

5579

9000

6892

4594

3842

9000

5812

936

848

1403

1063

32CV

J-2-5-3-1-3times(77371times

BSEC

TCP

-1)4345

3808

6774

4976

5272

4996

5035

5101

6650

5766

9000

7139

4288

3381

9000

5556

800

712

1013

841

33CV

J-2-5-3-1-3times96AC

-93

4862

4197

5743

4934

4884

4980

5058

4974

7477

6760

9000

7745

5957

4709

9000

6555

933

738

1478

1050

34CV

J-2-5-3-1-3timesTo

go-II

4670

4343

6233

5082

4399

4813

4631

4614

5806

6614

9000

7140

4422

4479

9000

5967

963

855

1180

999

35CV

J-2-5-3-1-3times99HS-18

5493

5080

7273

5949

5276

4984

5162

5141

7322

6590

9000

7637

5534

4766

9000

6433

998

807

1338

1047

361305times(77371times

BSEC

TCP

-1)

4613

4539

5717

4956

4986

4955

5080

5007

5137

4870

9000

6336

4210

3936

9000

5715

863

630

913

802

371305times96AC

-93

5473

5177

6830

5827

5224

4779

4919

4974

7117

6965

9000

7694

5640

5220

9000

6620

875

694

1476

1015

381305timesTo

go-II

4230

3420

5262

4304

4309

456144

45

4438

5781

4373

9000

6385

4575

2887

9000

5487

637

566

794

666

391305times99HS-18

5433

4507

6947

5629

5225

4753

4991

4989

7287

6968

9000

7752

5576

4479

9000

6352

1115

818

1410

1114

40(77371times

BSEC

TCP

-1)times

96AC

-93

5595

4429

7461

5828

5216

4955

5121

5097

7087

6368

9000

7485

5432

4038

9000

6157

955

753

1462

1057

41(77371times

BSEC

TCP

-1)times

Togo-II

5252

4728

6106

5362

5492

4808

5132

5144

8025

7505

9000

8177

6310

5413

9000

6908

859

693

990

848

42(77371times

BSEC

TCP

-1)times

99HS-18

5595

4298

6295

5396

4729

4887

5031

4883

7366

6945

9000

7770

6312

4556

9000

6623

903

622

1125

883

4396AC

-93timesTo

go-II

5017

3977

6486

5160

5019

5195

5237

5150

5922

5486

9000

6803

4689

3484

9000

5724

1007

728

1167

967

4496AC

-93times99HS-18

5595

4812

6924

5777

5036

5385

5205

5209

7411

5378

9000

7263

5873

3979

9000

6284

1013

652

1334

1000

45To

go-IItimes

99HS-18

4670

4497

7293

5487

4718

4319

4796

4611

5688

5431

9000

6706

3980

3665

9000

5548

939

653

1462

1018

Mean

5043

4482

6271

5265

4792

498149

23

4899

6377

584190

00

7072

5079

4190

9000

6090

898

701

1168

922

SE097

106

161

mdash213

191

190

mdash19

818

7mdash

mdash14

914

6mdash

mdash036

047

068

mdashCD

272

296

453

mdash597

537

534

mdash555

525

mdashmdash

421

409

mdashmdash

103

133

192

mdashlowastTransfo

rmed

data

(b)

Sr

No

Genotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

908

785

2245

1313

859

1537

1924

1440

154

143

160

152

6267

4800

3833

4967

212

227

249

229

15644

15458

13267

1479

02

H7729-2

1125

1175

1789

1363

317

2400

2811

1843

093

191

252

179

6700

5333

5100

5711

185

210

234

210

18850

18565

15389

17601

3G73-107

1166

1475

1966

1536

943

1744

4945

2544

143

129

366

213

6700

5433

5300

5811

218

219

238

225

15009

1496

513503

14492

477245

1438

1373

2085

1632

607

1863

5957

2809

117

180

509

269

6100

4800

5100

5333

232

221

253

235

14036

13802

11742

1319

35

CVJ-2-5-3-1-3

1214

1437

1807

1486

1163

1610

4166

2313

171

141

407

240

6300

4933

4600

5278

239

227

270

245

1994

317261

1497

017391

61305

858

1137

1737

1244

540

967

4861

2123

125

088

417

210

6533

5033

4800

5456

244

213

257

238

15527

15405

12274

14402

8 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

777371timesBS

ECT

CP-1

1377

1487

1892

1585

928

2660

3330

2306

150

258

301

236

5733

4533

4700

4989

242

220

293

252

1513

814550

1315

014279

896AC

-93

1303

1259

1852

1471

1056

1445

5972

2825

185

147

586

306

5500

4833

3833

4722

256

304

318

293

15577

14032

10000

13203

9To

go-II

1056

1220

2272

1516

924

1466

9113

3834

146

129

883

386

6167

4533

4600

5100

185

149

124

153

14578

12073

10033

12228

1099HS-18

1277

1075

1808

1387

1241

2833

7070

3715

228

226

615

356

6100

5000

4167

5089

243

173

324

247

12627

10355

1017

311052

1H77833-2times

H7729-2

1432

1498

2233

1721

1350

4405

8373

4709

159

499

782

480

5767

4433

3800

4667309

290

248

282

21010

18071

15847

18309

2H77833-2times

G73-107

1675

1635

2205

1838

1236

3211

5789

3412

134

313

547

331

5833

4767

3833

4811

262

295

236

265

21253

20449

15808

1917

0

3H77833-2times

77245

1577

1522

2263

1787

1058

6933

8719

5570

255

553

782

530

5267

4633

4600

4833

273

201

265

246

2074

119511

15208

18487

4H77833-2times

CVJ-2-5-3-1-3

1791

1757

2165

1904

20218030

5276

5109

354

779

477

537

5533

4233

3767

4511

165

330

351

282

21827

20072

17110

19670

5H77833-2times1305

1700

1842

28412128

1427

6228

9134

5596

570

579

813

654

5800

4267

3333

4467340

426

451

406

19816

19444

16037

18432

6H77833-2times

(77371times

BSEC

TCP

-1)

1453

1515

3120

2029

789

465510428

5291

211

408

858

492

5233

4167

3267

4222

203

283

267

251

20677

1913

816470

1876

2

7H77833-2times

96AC

-93

1835

1783

2427

2015

2532

6649

1610

684

29

350

650

1473

824

5467

4233

3700

4467243

239

256

246

22278

20434

15735

19482

8H77833-2times

Togo-II

1564

15632500

1876

1882

4772

11660

6105

296

469

972

579

5667

4533

440048

67

169

260

211

213

2412

82375

619389

22424

9H77833-2times

99HS-18

159318002049

1814

1571

480214024

6799

203

544

1547

765

6033

460040

0048

78

261

225

256

247

23466

21081

1793

320

827

10H7729-2times

G73-107

14951568

2075

1713

959

5157

8517

4877

218

382

666

422

6133

5067

4833

5344

300

273

239

271

23651

2191

717083

20884

11H7729-2times77245

1557

1537

2613

1902

1851

5756

5729

4445366

562

524

484

5633

4800

4633

5022

133

233

297

221

1979

219914

15250

18319

12H7729-2times

CVJ-2-5-3-1-3

18442033

28932257

1651

806114885

8199

264

597

1054

638

6333

4600

4233

5056

170

211

217

199

24629

23339

1970

022556

13H7729-2times1305

1325

1677309920

34

1756

724314048

7682

318

635

1232

728

630046

67

3633

4867298

261

361

307

21666

19371

17422

19486

14H7729-2times(77371

timesBS

ECTCP

-1)

1958

1887

3105

2317

1118

4675

14411

6734

242

457

1318

672

5900

4500

3800

4733

164

209

240

204

23661

1995

419250

20955

15H7729-2times

96AC

-93

1512

1717

2779

2002266844

08

11393

6156

356

500

1211

689

5633

4533

4200

4789

221

195

282

232

2194

921635

17213

20266

Advances in Agriculture 9

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

16H7729-2times

Togo-II

1519

1738

2159

1805

2437

9867

1616

294

89

361

961

1527

950

6167

4700

4200

5022

234

272

230

246

26338

2414

520553

23678

17H7729-2times

99HS-18

1938

1787

3151

2292

1574

6218

11318

6370

290

606

956

617

6067

4767

406749

67

172

210

199

194

24689

21854

1778

921444

18G73-107times77245

1856

1813

2210

1960

2435

8550

7828

6271338

633

612

528

5500

4633

440048

44

199

255

307

254

1993

619277

16095

18436

19G73-107times

CVJ-2-5-3-1-3

1517

1769

2407

1897

1556

7728

9136

6140

239

876

917

677

5900

4300

3400

4533

233

266

315

272

23418

22333

19317

21689

20G73-107times1305

1217

1507

2832

1852

1777

5189

1114

960

38

289

588

1197

691

6733

5000

4233

5322

286

210

312

269

2078

81992

817067

19261

21G73-107times(77371

timesBS

ECTCP

-1)

18551946

2730

2177

1801

3482

10415

5233

239

277

753

423

5500

4467

3433

4467243

197

273

238

23817

20263

17425

20502

22G73-107times

96AC

-93

1718

1840

28612140

1345

9333

14669

8449

297

845

1278

807

5600

5033

4633

5089

270

243

300

271

28537

21461

16875

22291

23G73-107timesTo

go-II

168018083087

2192

1112

4678

9319

5036

186

473

985

548

6033

4767

4200

5000

210

209

231

217

24864

24609

21625

23699

24G73-107times99HS-18

1398

1675

3370

2148

2753

8672

11918

7781

362

691

981

678

566746

00

3500

4589

170

247

270

229

24677

20567

17855

21033

2577245times

CVJ-2-5-3-1-3

1500

1728

2203

1810

2282

4679

11492

6151

328

424

1116

623

6000

4333

420048

44300

231

370

300

21257

20497

1772

519826

2677245times1305

143815772080

1698

949

10439

12053

7814

121

915

992

676

5767

4800

3500

4689

168246

316

244

1978

91779

015517

17699

2777245times(77371times

BSEC

TCP

-1)

150516972846

2016

3222

8518

12621

8120

469

827

1141

812

5400

4367

3833

4533

196

225

242

221

2092

819485

1719

219202

2877245times96AC

-93

1653

1797

2117

1856

1934

6056

11543

6511

352

563

911

608

5333

4267

3833

4478

186

169

198

184

22003

19054

1676

719275

2977245timesTo

go-II

1723

1718

3163

2201

1749

8250

15443

8481229

806

1412

816

5967

4700

4067

4911

174

245

298

239

22075

23503

19875

21818

3077245times99HS-18

2016

1970

28542280

3217

13027

3531

6591395

965

285

549

5733

4467

3833

4678

148

228

209

195

2212

721462

1690

020

163

31CV

J-2-5-3-1-3times

1305

1358

1422

2179

1653

1254

2803

1019

44750

178

254

801

411

6033

4467

3367

4622

312

193

330

278

22269

19547

17250

19689

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

1335

1742

2400

1826

922

5226

12806

6318

236

458

1054

583

5633

4433

3600

4556

321

315

259

298

2210

419524

17087

19572

33CV

J-2-5-3-1-3times

96AC

-93

1552

16803260

2164

166710094

11941

7901250

974

1115

779

5533

4533

446748

44

170

202

317

230

2392

123317

19333

2219

0

34CV

J-2-5-3-1-3times

Togo-II

142216072638

1889

1012

4163

11061

5412

261

377

891

510

6167

4967

4533

5222

207

225

325

252

23590

22860

20278

22243

35CV

J-2-5-3-1-3times

99HS-18

1435

1600

2700

1912

3056

3237

7089

4461449

301

658

469

5700

44673800

4656

148

178

225

183

22444

20240

1696

719884

361305times(77371times

BSEC

TCP

-1)

1499

1767

2058

1774

1882

4665

7920

4822

356

426

724

502

566742673800

4578

271

242

305

273

22011

19295

16417

19241

371305times96AC

-93

16781605

2757

2014

1516

10037

11960

7838

311

11381284

911

560040

673400

4356

182

276

251

236

2396

519625

15800

1979

738

1305timesTo

go-II

1177

16802249

1702

902

4676

9388

4989

241

456

795

497

6533

5533

4733

5600

214

266

296

259

21894

20217

19222

20444

391305times99HS-18

1774

1767

2766

2102

2145

4628

10618

5797

301

447

991

580

5933

4500

3733

4722

128

142

180

150

2275

719413

16967

1971

2

40(77371times

BSEC

TCP

-1)times

96AC

-93

1748

1795

31712238

1537

12586

14379

9501

211

1104

1183

833

546744

00346744

44

127

201

226

185

23294

21089

17858

2074

7

10 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(

days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

41(77371times

BSEC

TCP

-1)times

Togo-II

19652007

2653

2208

2136

5601

9503

5747

315

635

1001

650

5533

4833

410048

22

213

241

286

247

22662

22094

17667

20808

42(77371times

BSEC

TCP

-1)times

99HS-18

15011687

2705

1964

1935

7253

11806

6998

314

704

1067

695

5467

4433

5133

5011

177

167

175

173

22305

19277

16392

19325

4396AC

-93timesTo

go-II1759

1618

2123

1834

1539

10347

12212

8033

325

907

1090

774

5900

450039674789

214

269

339

274

23252

21554

16875

20560

4496AC

-93times

99HS-18

1353

1618

2925

1966

3532

7223

11950

7569

440

654

1024

706

5600

4900

4533

5011

149

175

206

177

24494

20698

18111

2110

145

Togo-IItimes

99HS-18

1117

1278

2582

1659

3448

6764

11910

7374

477

767

1207

817

6000

5133

4333

5156

183

203

216

201

17683

15541

14333

15853

Mean

1513

1618

2492

1874

1656

5773

9854

5761

272

538

885

565

586846

534116

4879

218

233

268

240

21297

19547

16529

1912

4SE

101

073

098

mdash079

152

317

mdash014

014

028

mdash351

365

428

mdash036

039

046

mdash985

1173

1211

mdashCD

284

205

276

mdash221

427

890

mdash038

040

079

mdash12

513

015

2mdash

013

014

016

mdash351

418

431

mdash

(c)

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

1642

1667

1744

1684

1504

1349

1526

1460

2783

2405

2634

2607

836

745

908

830

4287

3754

4160

4067

2H7729-2

1693

1817

1950

1820

1012

859

1016

962

3433

2809

2591

2944

649

597

651

632

4445

3668

3607

3906

3G73-107

2000

2028

2077

2035

2280

1966

2312

2186

4695

4023

4229

4316

1281

1170

1463

1305

6975

5989

6541

6502

477245

1523

1630

1728

1627

1796

2489

2702

2329

5423

4851

4498

4924

748

1464

1743

1319

7219

7341

7200

7253

5CV

J-2-5-3-1-3

2282

2692

2787

2587

2411

2643

2901

2652

5857

4836

5024

5239

1206

1335

1769

1437

8268

7479

7925

7891

61305

1851

1951

2054

1952

2026

1763

1908

1899

4657

4414

4463

4511

1468

1336

1539

1448

6683

6177

6371

6410

777371timesBS

ECTCP

-11629

1645

1733

1669

1244

1692

1884

1607

4925

4183

4435

4515

625

1092

1483

1067

6169

5875

6319

6121

896AC

-93

1379

1519

1600

1499

2066

2942

3088

2698

4501

4095

4229

4275

984

1565

1678

1409

6567

7037

7316

6973

9To

go-II

1904

2057

2103

2021

2167

1844

2358

2123

4520

3446

3904

3957

1298

1138

1502

1313

6687

5290

6262

6080

1099HS-18

1475

1485

1633

1531

3180

2904

3382

3155

5633

4139

5205

4992

1656

1536

1879

1690

8813

7043

8587

8147

1H77833-2timesH7729-2

2010

2046

2133

2063

3645

3075

4377

3699

7675

6540

7199

7138

2014

1708

2880

2201

11320

9615

11576

10837

2H77833-2timesG73-107

2066

2101

2122

2096

5002

4053

4601

4552

7882

7430

6963

7425

3146

2565

2808

2840

12884

11482

11564

11977

3H77833-2times77245

1764

2048

2083

1965

3769

4497

4559

4275

8681

8515

8475

8557

2284

3356

3377

3006

12450

13012

13034

12832

4H77833-2times

CVJ-2-5-3-1-3

2205

2383

2733

2441

3693

5655

5220

4856

11656

10287

1010

510683

2198

4488

4176

3621

15348

1594

215325

15538

5H77833-2times1305

2209

2423

2491

2374

6008

5842

6933

6261

15238

14311

13889

14479

3758

4057

5591

4469

21246

2015

320822

2074

0

6H77833-2times(77371times

BSEC

TCP

-1)

1789

1823

1992

1868

3593

4288

4903

4262

8922

6522

7429

7625

2178

3224

3070

2824

12515

10811

12333

11886

7H77833-2times96AC

-93

1739

1960

2011

1903

4557

4350

4818

4575

7916

6068

7290

7092

2411

2529

3191

2710

12474

10419

1210

811667

8H77833-2timesTo

go-II

1927

2389

2406

2241

4185

5828

6215

5409

12319

10279

11023

11207

2433

4254

4631

3773

16504

1610

717238

16616

9H77833-2times99HS-18

1941

2245

2330

2172

4227

4139

4529

4298

9211

8120

7969

8433

3154

3184

3253

3197

13438

12259

12498

1273

210

H7729-2timesG73-107

1968

2109

2158

2079

3725

3042

3488

3418

7313

5956

6174

6481

2822

2001

2357

2393

11038

8998

9662

9899

Advances in Agriculture 11

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

11H7729-2times77245

1847

2048

2200

2032

2467

3759

5466

3898

7672

7449

8921

8014

1356

2350

3796

2500

1013

911208

14387

11911

12H7729-2times

CVJ-2-5-3-1-3

2218

2585

2767

2523

3674

4372

4483

4176

10053

9467

8664

9395

2417

3312

3345

3025

1372

813839

1314

713571

13H7729-2times1305

2169

2338

2547

2352

6728

6166

6792

6562

13333

11662

13301

1276

54128

4501

5660

4763

20061

17828

20093

19327

14H7729-2times(77371times

BSEC

TCP

-1)

1925

2095

2127

2049

3530

4165

4649

4115

9638

6917

7698

8084

1681

2833

3150

2555

1316

8110

82

12347

1219

915

H7729-2times96AC

-93

2012

2070

2180

2087

4955

4447

4596

4666

8738

6882

7762

7794

3754

3558

3181

3498

13693

11329

12359

12460

16H7729-2timesTo

go-II

2138

2428

2563

2377

4949

4660

5118

4909

8549

7243

7191

7661

3586

3353

3028

3322

13498

11903

12310

12570

17H7729-2times99HS-18

1899

1933

2073

1969

4046

4527

4988

4520

8492

7895

8144

8177

2561

3378

3553

3164

12539

12422

1313

212698

18G73-107times77245

1840

2253

2437

2177

2580

4776

5915

4424

11673

10608

10459

10913

1358

3163

4286

2936

14253

15385

16374

15337

19G73-107times

CVJ-2-5-3-1-3

2324

2537

2691

2517

4958

5030

5599

5196

9524

9395

1114

610022

3241

3645

4444

3776

14483

14425

1674

515217

20G73-107times1305

2228

2433

2583

2415

6340

5794

6373

6169

12490

1015

8119

61

11536

4199

4199

5018

4472

18830

1595

218334

1770

6

21G73-107times(77371times

BSEC

TCP

-1)

1943

2128

2220

2097

3936

3980

5770

4562

11697

9858

10303

10619

1874

2369

4181

2808

15633

13839

16073

1518

222

G73-107times96AC

-93

2118

2218

2317

2217

4270

5843

6201

5438

10319

9872

1015

910117

2703

4460

4379

3847

14589

15715

16359

15555

23G73-107timesTo

go-II

2237

2365

2733

2445

5736

5175

6822

5911

12098

10808

1299

611967

3984

3670

5372

4342

17835

1598

219818

17878

24G73-107times99HS-18

2025

2081

2322

2143

3558

4229

6612

4800

12350

9685

11516

1118

42196

2782

5009

3329

1590

813914

1812

915983

2577245timesCV

J-2-5-3-1-3

2109

2360

2675

2381

6760

6558

7299

6872

15225

11861

1416

21374

94225

4752

5839

4939

2198

518418

21461

20621

2677245times1305

1919

2100

2197

2072

5700

5142

5347

5397

11687

10565

10737

10996

4318

3956

4244

4173

17386

1570

816085

16393

2777245times(77371times

BSEC

TCP

-1)

1851

1935

2020

1936

2577

2460

2838

2625

6989

4778

4636

5468

1631

1662

1999

1764

9566

7238

7474

8093

2877245times96AC

-93

1729

1780

2133

1881

5541

5058

5748

5449

11952

8174

9526

9884

3646

3587

4071

3768

17493

13232

15274

15333

2977245timesTo

go-II

2300

2508

2658

2489

3067

5431

5963

4820

11737

11021

1216

411641

1598

4053

4770

3474

14805

16452

1812

616461

3077245times99HS-18

1982

2019

2223

2075

3846

3119

4225

3730

7493

6720

6476

6896

3205

2578

2643

2809

11339

9839

1070

110626

12 Advances in Agriculture

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

31CV

J-2-5-3-1-3times1305

2406

2553

3067

2675

4178

4796

6281

5085

13822

11425

12252

12500

2472

3241

4907

3540

18000

16222

18532

17585

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

2142

2173

2238

2184

4522

5235

5640

5132

11673

9658

10248

10526

2614

3766

4178

3519

1619

514893

15888

15659

33CV

J-2-5-3-1-3times

96AC

-93

2350

2477

2600

2476

4676

4280

6090

5015

11975

9832

10868

10891

3097

2931

4445

3491

16651

14111

1695

815907

34CV

J-2-5-3-1-3times

Togo-II

2093

2666

2989

2583

4058

3727

5190

4325

12588

10717

11217

11507

2742

2570

4185

3166

16645

14443

16407

15832

35CV

J-2-5-3-1-3times

99HS-18

2028

2311

2386

2242

3287

2513

5356

3719

10055

9733

1036

410051

2004

1581

4251

2612

13342

12246

1572

01376

9

361305times(77371times

BSEC

TCP

-1)

2005

2173

2210

2129

3569

4692

5140

4467

11652

9826

10041

10507

2005

3665

4179

3283

15221

14518

1518

214974

371305times96AC

-93

1822

2003

2047

1957

5115

4023

4813

4650

9965

7675

7571

8404

3456

2647

3155

3086

15080

11698

12384

13054

381305timesTo

go-II

2545

2583

2644

2591

6885

7283

6795

6987

12265

11410

12583

12086

4500

6120

5350

5323

1915

018693

19378

19074

391305times99HS-18

2070

2408

2593

2357

5219

4332

4800

4784

1092

97677

8968

9192

3924

3332

3840

3699

1614

912009

1376

913976

40(77371times

BSEC

TCP

-1)

times96AC

-93

2056

2115

2157

2109

3158

4463

4717

4113

9166

7540

8100

8268

1880

3542

3347

2923

12324

12003

12817

12381

41(77371times

BSEC

TCP

-1)

timesTo

go-II

1868

2253

2450

2190

4422

6458

7038

5973

1415

312369

1379

713439

2291

4646

5586

4174

18575

18827

20835

19412

42(77371times

BSEC

TCP

-1)

times99HS-18

1995

2026

2209

2077

2720

2909

3543

3057

7392

4692

5023

5702

1766

2187

2147

2033

10112

7601

8566

8760

4396AC

-93timesTo

go-II

1953

2027

2511

2164

5972

4391

7126

5830

15537

1392

714432

14632

3780

2538

5655

3991

21510

18318

21558

20462

4496AC

-93times99HS-18

1952

2021

2242

2072

3465

2758

4496

3573

5959

5186

6094

5746

2707

2154

2409

2423

9424

7944

10590

9319

45To

go-IItimes

99HS-18

1932

2008

2658

2199

5381

4995

5436

5270

10299

8876

9244

9473

3986

3842

3668

3832

15680

13871

14680

1474

3Mean

1982

2146

2301

2143

3963

4123

4836

4307

9498

8087

8663

8749

2510

2914

3513

2979

13460

12210

13499

13056

SE243

198

245

mdash671

501

626

mdash959

750

822

mdash423

349

344

mdash1155

927

1167

mdashCD

087

071

087

mdash239

178

223

mdash342

267

293

mdash15

112

412

2mdash

411

330

416

mdash

Advances in Agriculture 13Ta

ble4Analysis

ofvaria

ncefor

10times10

half-diallelfor

different

charactersin

threee

nviro

nments

(a)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Germination

()

Rateof

emergence

STI()

SSTI

()

Num

bero

fleavesseedling

Seedlin

gheight

(cm)

Freshweight

(g)seedlin

gDry

weight

(g)seedlin

g

E 1

Replicates

2652731

596768

783393

20700

93018674

33764

1033999

002590

Treatm

ents

541097

0255lowastlowast

8181153lowastlowast

30863092lowastlowast

20680900lowastlowast

1100708lowastlowast

2106317lowastlowast

930829527lowastlowast

319767lowastlowast

Parents

95118

163lowastlowast

14289925lowastlowast

59114816lowastlowast

18880254lowastlowast

1084227lowastlowast

1087906lowastlowast

22561719lowastlowast

042162lowastlowast

Hybrid

s44

11423573lowastlowast

3398933lowastlowast

25697199lowastlowast

20633716lowastlowast

472874lowastlowast

1391526lowastlowast

674123518lowastlowast

261839lowastlowast

Parentsv

ersush

ybrid

s1

43693104lowastlowast

163619

916lowastlowast

3896838

3896

2793lowastlowast

28873741lowastlowast

4272

2798lowastlowast

234144289lowastlowast

5367022lowastlowast

Error

108

281184

1356832

1173684

674337

040104

306

462

2015

1619

005535

E 2

Replicates

2583819

3707389lowast

1596635

198738

034925

448213

899529

007177

Treatm

ents

549997443lowastlowast

2825177lowastlowast

35378177lowastlowast

19074306lowastlowast

665284lowastlowast

1764

081lowastlowast

257694570lowastlowast

2177519lowastlowast

Parents

96507784lowastlowast

3718

879lowastlowast

69866289lowastlowast

1798

5404lowastlowast

705379lowastlowast

1408518lowastlowast

10612519lowastlowast

077429lowastlowast

Hybrid

s44

10569731lowastlowast

2127302lowastlowast

2910

2537lowastlowast

19429240lowastlowast

329256lowastlowast

7000

03lowastlowast

186026552lowastlowast

1483428lowastlowast

Parentsv

ersush

ybrid

s1

16223710lowastlowast

25488361lowastlowast

1113

326

13257362lowastlowast

15089673lowastlowast

5178

3569lowastlowast

5634825791lowastlowast

51618348lowastlowast

Error

108

334760

1097039

1049505

635570

06764

116

0576

694613

006206

E 3

Replicates

2894398

4808815lowast

mdashmdash

051076

095989

3768421

030220

Treatm

ents

541261560

0lowastlowast

4842216lowastlowast

mdashmdash

2811077lowastlowast

5947316lowastlowast

3870844

50lowastlowast

3445076lowastlowast

Parents

97135

735lowastlowast

1097

6388lowastlowast

mdashmdash

1468699lowastlowast

1112

764lowastlowast

136081650lowastlowast

1307836lowastlowast

Hybrid

s44

13217740lowastlowast

1379360

mdashmdash

15540

71lowastlowast

4581632lowastlowast

252084767lowastlowast

2378032lowastlowast

Parentsv

ersush

ybrid

s1

35440

180lowastlowast

102000335lowastlowast

mdashmdash

70200767lowastlowast

109548362lowastlowast

8586095705lowastlowast

69630188lowastlowast

Error

108

782907

1085444

mdashmdash

139771

291325

3019

169

02400

4(b)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Panicle

emergence

Effectiv

etillersplant

Planth

eight

Earlength

Earw

eightp

lant

Dry

fodd

eryieldplant

Grain

yieldplant

Totalbiological

yieldplant

E 1

Replicates

231860

61lowastlowast

416348lowastlowast

1672

9472lowast

1706

411077192

9634539

392777

115544

59Treatm

ents

544152

368lowastlowast

085657lowastlowast

326706089lowastlowast

1602572lowastlowast

6177

0406lowastlowast

304113

204lowastlowast

34285030lowastlowast

575617

582lowastlowast

Parents

94511481lowastlowast

018538lowastlowast

140807476lowastlowast

2240093lowastlowast

11653

959lowastlowast

27014155lowastlowast

3858650lowastlowast

61321919lowastlowast

Hybrid

s44

3201347lowastlowast

100797lowastlowast

110395657lowastlowast

1010

497lowastlowast

40296671lowastlowast

171271411lowastlowast

2413

6880lowastlowast

302999650lowastlowast

Parentsv

ersush

ybrid

s1

4276

5286lowastlowast

02360

9lowast11517452

616lowastlowast

2191

6167lowastlowast

1457662749lowastlowast

8643043524lowastlowast

7546

41053lowastlowast

17199467546lowastlowast

Error

108

468159

004907

3692099

224610

17114

303498126

682350

5075268

E 2

Replicates

2656364

002556

61575565lowastlowast

828842lowastlowast

589094

2268506

260877

1507116

Treatm

ents

542999618lowastlowast

069822lowastlowast

268998723lowastlowast

2436886lowastlowast

60592784lowastlowast

247259877lowastlowast

4192

0073lowastlowast

513483489lowastlowast

Parents

926300

00lowastlowast

048079lowastlowast

165555

405lowastlowast

3871874lowastlowast

1412

2393lowastlowast

19543682lowastlowast

3132

426lowastlowast

57230706lowastlowast

Hybrid

s44

2533

165lowastlowast

07344

9lowastlowast

9616

2857lowastlowast

1463359lowastlowast

35495366lowastlowast

154759288lowastlowast

26262465lowastlowast

293492304lowastlowast

Parentsv

ersush

ybrid

s1

26850135lowastlowast

105929lowastlowast

880476

6697lowastlowast

32357161lowastlowast

1583112746lowastlowast

6366731560lowastlowast

107994

3646lowastlowast

14299370699lowastlowast

Error

108

507598

005829

5234122

149406

955133

2142411

462311

3273886

E 3

Replicates

2029697

021161

9459847

143746

985141

46800

6492

6334

3345160

Treatm

ents

548045499lowastlowast

094511lowastlowast

192420108lowastlowast

3279225lowastlowast

72049211lowastlowast

298953

021lowastlowast

55061308lowastlowast

651732994lowastlowast

Parents

98010

741lowastlowast

094166lowastlowast

116037343lowastlowast

3702185lowastlowast

16305190lowastlowast

2323644

0lowastlowast

4553

893lowastlowast

71484982lowastlowast

Hybrid

s44

6254377lowastlowast

095514lowastlowast

7378

1513lowastlowast

2188348lowastlowast

31813193lowastlowast

190263916lowastlowast

31572179lowastlowast

368689934lowastlowast

Parentsv

ersush

ybrid

s1

8716

7710lowastlowast

053496lowast

609996

3199lowastlowast

47471212lowastlowast

2344

130203lowastlowast

756272

2861lowastlowast

154314

9707lowastlowast

18327859

719lowastlowast

Error

108

696981

007883

5578505

228608

1491014

2574

223

449588

5187705

lowast

Sign

ificant

at119875=005

lowastlowastSign

ificant

at119875=001

14 Advances in Agriculture

Table 5 Mean performance of the genotypes for membrane thermostability in two environments

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594

Advances in Agriculture 15

Table 5 Continued

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434

Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash

lowastValues are transformed values

0

6

12

18

24

30

36

42

48

54

minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity

G73-10796AC-93CVJ-2-5-3-1-3

Togo-II99HS-18

H77833-2H7729-2772451305

H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305

1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305

H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305

77245 times 1305

77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305

77371 times BSECT CP-1

H77833-2 times G73-107

Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits

moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling

survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The

16 Advances in Agriculture

Table6Po

oled

correla

tioncoeffi

cientamon

gvario

uscharactersin

threee

nviro

nments

Characters

Germination

Rateof

emergence

STI

SSTI

Leaves

seedlin

gSeedlin

gheight

Fresh

weight

Dry

weight

MTS

Panicle

emergence

Effectiv

etillersplant

Plant

height

Earlength

Ear

weightp

lant

Dry

fodd

eryield

plant

Grain

yield

plant

Total

biological

yield

plant

Germination

1000

0357lowastlowast

0353lowastlowast

0590lowastlowast

0230

0373lowastlowast

0293lowast

0260

0276lowastminus0135minus0478lowastlowast

0326lowast

minus0069minus0104

minus0190

minus0145

minus0167

Rateof

emergence

1000

0233

0351lowastlowast

0605lowastlowast

0585lowastlowast

0551lowastlowast

0524lowastlowast

0139minus0593lowastlowast

0032

0517lowastlowast

0217

0388lowastlowast

0361lowastlowast

0348lowastlowast

0377lowastlowast

STI

1000

0837lowastlowast

0241

0129

0081

004

80890lowastlowast

minus0215

minus0211

0030minus0007minus0043

minus0101

minus0111

minus0084

SSTI

1000

0233

0264

0183

0140

0745lowastlowast

minus0247minus0268lowast

0184minus0017minus006

6minus0129

minus0144

minus0112

Num

bero

fleaves

1000

0683lowastlowast

0633lowastlowast

060

4lowastlowast

0176minus0474lowastlowast

0109

0576lowastlowast

0496lowastlowast

064

1lowastlowast

0607lowastlowast

0630lowastlowast

0630lowastlowast

Seedlin

gheight

1000

0703lowastlowast

0663lowastlowast

0031minus0493lowastlowast

minus0115

0735lowastlowast

0360lowastlowast

0526lowastlowast

0520lowastlowast

0515lowastlowast

0532lowastlowast

Freshweight

1000

0957lowastlowast

minus0016

0425lowastlowast

minus0082

0661lowastlowast

0351lowastlowast

0581lowastlowast

0496lowastlowast

0576lowastlowast

0533lowastlowast

Dry

weight

1000minus0033minus0428lowastlowast

minus0016

0634lowastlowast

0354lowastlowast

0620lowastlowast

0509lowastlowast

0614lowastlowast

0554lowastlowast

MTS

1000minus040

minus0177minus0037

0015

minus0074

minus0138

minus0131

minus0120

Panicle

emergence

1000

minus0150minus0269lowast

0022minus0329lowast

minus0337lowast

minus0280lowast

minus0341lowast

Effectiv

etillersplant

1000

minus0012

0170

0366lowastlowast

040

5lowastlowast

0323lowast

0401lowastlowast

Planth

eight

1000

0650lowastlowast

0603lowastlowast

0596lowastlowast

0619lowastlowast

0610lowastlowast

Earlength

1000

0608lowastlowast

0671lowastlowast

064

8lowastlowast

066

4lowastlowast

Earw

eightp

lant

1000

0910lowastlowast

0984lowastlowast

0957lowastlowast

Dry

fodd

eryield

plant

1000

0907lowastlowast

0991lowastlowast

Grain

yield

plant

1000

0950lowastlowast

Totalbiological

yield

plant

1000

lowastSign

ificant

at119875=005lowastlowastSign

ificant

at119875=001

Advances in Agriculture 17

faster germination and early vigour in seedling could act asphysical indicators for heat tolerance

Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits

Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics

Conflict of Interests

The authors declare that there is no conflict of interestsregarding the publication of this paper

References

[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981

[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993

[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006

[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993

[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013

[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007

[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965

[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011

[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001

[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009

[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962

[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967

[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963

[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001

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Page 5: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

Advances in Agriculture 5

the software SPSS (SPSS Inc) Multivariate clustering wasdone based on paired group cluster method using similaritycoefficient under Past 140 software [14] Multivariate analysisbased on the Euclidean distancematrix was estimated amonggenotypes to distinguish genotypes from each other Thegrouping of genotypes was generated using paired groupcluster method

3 Results and Discussion

The excessive heat (soil temperature up to 630∘C in E2)

about 18∘C higher than the normal atmospheric temperature(Figures 1(a) and 1(b)) was responsible for mortality ofseedlings Many seedlings were found desiccated at the pointof contact besides the desiccation at the tipsThe temperaturearound the tip level (5 cm above the soil surface) also reachedup to 50∘C (Figure 1(c)) It could therefore be concluded thatthe seedlings had to survive in a really high temperature Themoisture level remained above the permanent wilting point(Figure 1(d)) therefore drought effect was not in the playThe heat tolerance was measured as STI (Table 3) throughthe field screening technique of Peacock et al [4] Anotherindex known as SSTI was also calculated as an improvementand extensionThe analysis of variance (RBD) for 8 charactersin 3 environments showed significant genotypic differencesNo variability was observed for STI and SSTI in E

3only In

this environment no seedling mortality was observed andthe germination was taken as standard The values for STIand SSTI were therefore equal and the highest for all thegenotypes in this environment and hence no variation wasobserved

The hybrids were evaluated on the basis of mean perfor-mance for various characters in each environment (Table 3)On the mean basis STI value ranged from 5332 to 8224with an average of 7072 The hybrid H7729-2 times CVJ-2-5-3-1-3 was the best scorer for heat tolerance followed byH77833-2 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II The hybrid H77833-2 times 1305 was found susceptible toheat stress Seed to seedling thermotolerance index (SSTI)which is expected to be more rigorous index deviated thevalues from STI according to variation in germination undernormal environment (E

3) as the SSTI also coversincludes

the under surface mortality (USM) during germinationThe reductions in values were not strictly proportional tothe STI The reduction is dependent on thermosensitivityof germination We also came across such hybrids whichshowed poor germination and high STI SSTI values rangedfrom 4605 to 7287 with an average of 6090 The genotypeH77833-2 times 96AC-93 had the highest worth for SSTI onmean basis followed byG73-107times (77371timesBSECTCP-1) andH77833-2 times (77371 times BSECT CP-1) (Table 3) The analysisof variance for ten different characters in three differentenvironments (two stressed and one normal) was carried outand the results showed significant genotypic differences forall the characters (Table 4)

A caution is needed for evaluating such genotypes underSTI The SSTI gave a highly reduced value putting thesecrosses in the susceptible list It may be concluded therefore

that SSTI is more dependable and desirable tool for screeningand evaluating the genotypes for heat tolerance The germi-nation was lower in E

2than in E

1 This period has the highest

range of temperature with a high average Genotype-wise thehighest germination (6209) was recorded for the genotypeH7729-2 times (77371 times BSECT CP-1) on mean basis whichgives a preliminary indication of resistance to heat while thegenotype 1times 6 had the lowest germination (3533)The hybridH77833-2 times G73-107 was adjudged as the fastest emergenceof seedlingsThe genotype (77371timesBSECTCP-1)times 96AC-93had the highest fresh weightseedlingThe genotype H7729-2 times Togo-II had the highest seedling dry weight E

1had lower

dry weightseedling than E2indicating the more heat stress

during the E1

31 Membrane Thermostability Membrane thermostability(MTS) has long been recognised as a parameter of heattolerance All the hybrids were put to membrane thermosta-bility test in the laboratory under standard protocol Acompletely randomised design (CRD) analysis was carriedout for membrane thermostability (MTS) The mean sum ofsquares due to genotypes was highly significant indicatingthat enough genetic variability was present for membranethermostability (MTS) in each environment which justifiedfurther analysis of the data The range of values lay between5073 and 7742 Among the parents H77833-2 G73-107CVJ-2-5-3-1-3 77371 times BSECT CP-1 96AC-93 Togo-II and99HS-18 were verified as resistant genotypes and H7729-2 77245 and 1305 genotypes were found susceptible asmentioned in Table 1 (on the basis of previous experiment)Among all the hybrids H7729-2 times CVJ-2-5-3-1-3 CVJ-2-5-3-1-3 times 96AC-93 and (77371 times BSECT CP-1) times Togo-II werethree highly tolerant genotypes on the basis of membranethermostability (Table 5)

32 Correlations The pooled correlations among the charac-ters were calculated (Table 6) around heat tolerance indices(STI SSTI and membrane thermostability) with a view tofind whether there was any association of these characterswith seedling as well as maturity traits It was observedthat STI and SSTI recorded the highest significant cor-relation among themselves as they are related indices forheat tolerance Membrane thermostability also showed highsignificant correlation with STI as well as SSTI STI hada significant positive association with germination of seedonly among seedling and maturity traits studied SSTI inaddition was also positively correlated with germination andemergence rate and negatively associated with number ofeffective tillersplantMembrane thermostability also showedsignificant positive correlation with seed germination amongseedling and maturity traits studied While evaluating thediallel progenies it was also seen that the heat toleranceindices STI SSTI and membrane thermostability were notshowing any perceptible correlation with the rest of thedevelopmental traits (seedling andmaturity traits)The stressperiod was terminated by irrigating the field experiment after15 days fromdate of sowingwhen the seedlings get establishedand further mortality stopped due to heat Moreover the soil

6 Advances in Agriculture

Table3Perfo

rmance

ofthep

arentsandthec

rosses

ford

ifferentcharacters

(a)

Sr

No

Genotypes

Germination(

)Ra

teof

emergencelowast

STIlowast

()

SSTIlowast(

)Num

bero

fleavesseedlin

gE 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

3941

3737

6164

4614

4037

4109

4279

4142

7830

7387

9000

8072

4498

4133

9000

5877

661

502

800

654

2H7729-2

5513

4726

6092

5443

2815

4980

3258

3684

4835

4495

9000

6110

4441

3602

9000

5681

363

294

427

361

3G73-107

5037

4826

5918

5260

4534

4132

4481

4382

6187

5413

9000

6866

5213

4460

9000

6224

878

710

935

841

477245

4747

4624

5565

4978

3625

4604

4047

4092

4385

3580

9000

5655

3803

3040

9000

5281

377

292

437

369

5CV

J-2-5-3-1-3

4824

4832

6897

5518

5044

4907

4960

4970

7193

6813

9000

7669

4959

4785

9000

6248

802

580

923

768

61305

4326

3891

5404

4540

3376

5056

3573

4002

3903

3281

9000

5395

3211

2452

9000

4888

350

284

414

349

777371timesBS

ECTCP

-14747

4502

6320

5190

4934

5075

5065

5025

6952

6706

9000

7552

5056

4671

9000

6242

621

542

700

621

896AC

-93

4651

3818

5722

4730

4312

4915

4753

4660

6660

6001

9000

7220

5212

3920

9000

6044

701

590

899

730

9To

go-II

4613

3937

5276

4609

4245

4737

4797

4593

7198

6861

9000

7686

5980

4767

9000

6582

681

629

853

721

1099HS-18

4575

3823

6247

4882

4317

4659

4746

4574

7594

7319

9000

7971

5154

4172

9000

6109

745

554

915

738

1H77833-2timesH7729-2

5787

5295

6733

5938

4964

5015

4989

4989

6803

6504

9000

7436

5808

5134

9000

6647

711

585

780

692

2H77833-2timesG73-107

5472

4417

6405

5431

5448

5422

5239

5370

7231

6769

9000

7667

5980

4556

9000

6512

1101

705

1340

1049

3H77833-2times77245

4076

3707

5504

4429

5217

5446

5238

5300

6627

6224

9000

7284

4690

4037

9000

5909

740

525

965

744

4H77833-2timesCV

J-2-5-3-1-3

4843

4300

5856

5000

4862

4956

4913

4911

7061

6519

9000

7527

5575

4632

9000

6403

998

885

1258

1047

5H77833-2times1305

3296

2780

4521

3533

5076

4907

5238

5074

3732

3265

9000

5332

2765

2050

9000

4605

1192

934

1600

1242

6H77833-2times(77371times

BSEC

TCP

-1)5636

5539

6258

5811

4489

5219

5015

4908

6981

6482

9000

7488

6146

5680

9000

6942

898

726

1525

1050

7H77833-2times96AC

-93

5720

5515

6335

5857

4800

5186

5075

5021

7833

7786

9000

8206

6603

6256

9000

7287

1107

873

1193

1058

8H77833-2timesTo

go-II

4862

3453

5709

4675

5036

4829

4911

4925

6190

5500

9000

6897

5214

3339

9000

5851

979

670

1107

919

9H77833-2times99HS-18

4575

4418

5447

4813

5041

5074

5006

5040

5800

5073

9000

6625

4846

4133

9000

5993

978

733

1135

949

10H7729-2timesG73-107

5615

4962

6273

5617

5023

5535

5165

5241

6143

5501

9000

6881

5540

4441

9000

6327

1000

720

1363

1028

11H7729-2times77245

5472

5164

6281

5639

4745

5031

5028

4935

5572

5175

9000

6582

4901

4345

9000

6082

880

620

897

799

12H7729-2timesCV

J-2-5-3-1-3

5494

5124

6536

5718

4804

5316

4957

5026

7596

8076

9000

8224

6093

5680

9000

6924

981

932

1451

1121

13H7729-2times1305

3804

3826

5511

4380

3596

4724

4387

4236

6362

7075

9000

7479

4230

4517

9000

5916

926

725

1497

1049

14H7729-2times(77371times

BSEC

TCP

-1)

6369

4847

7412

6209

4847

5351

5246

5148

6377

6031

9000

7136

5683

4230

9000

6304

977

671

1380

1009

15H7729-2times96AC

-93

5314

4657

6702

5558

4514

5295

5102

4971

5612

4856

9000

6489

4613

3603

9000

5739

846

679

1397

974

16H7729-2timesTo

go-II

5827

5594

6651

6024

5072

5279

5271

5207

6563

5371

9000

6978

5763

4652

9000

6472

873

719

1040

877

17H7729-2times99HS-18

5741

4638

7918

6099

4847

5024

5021

4964

6179

5603

9000

6927

4882

3725

9000

5869

1012

730

1600

1114

18G73-107times77245

5115

4646

6103

5288

4977

5242

5450

5223

7006

6063

9000

7356

5685

4594

9000

6426

1021

790

1292

1034

19G73-107timesCV

J-2-5-3-1-3

5134

4894

6427

5485

4901

5317

5181

5133

6637

6124

9000

7254

5253

4710

9000

6321

1039

863

1254

1052

20G73-107times1305

4881

4561

7597

5680

4717

4791

4775

4761

6704

6380

9000

7361

4556

4115

9000

5890

1140

882

1657

1226

21G73-107times(77371times

BSEC

TCP

-1)

5636

5519

6283

5812

4986

5219

5203

5136

7990

6203

9000

7731

6620

5436

9000

7019

977

703

1395

1025

22G73-107times96AC

-93

5740

4510

6415

5555

5129

4982

5195

5102

6978

5556

9000

7178

6156

4019

9000

6392

1077

860

1264

1067

23G73-107timesTo

go-II

4460

4357

6044

4953

4396

5138

4951

4829

5279

4835

9000

6371

4018

3603

9000

5540

906

881

1189

992

24G73-107times99HS-18

5473

4356

6374

5401

5102

4955

5055

5037

6460

5197

9000

6886

5534

3704

9000

6079

1171

766

1635

1191

2577245timesCV

J-2-5-3-1-3

4766

4666

5921

5117

5170

4918

4900

4996

5928

5859

9000

6929

4785

4613

9000

6133

984

920

1194

1033

2677245times1305

4575

4060

6052

4895

5128

5297

5203

5210

5102

4990

9000

6364

3979

3464

9000

5481

1279

823

1550

1217

2777245times(77371times

BSEC

TCP

-1)

5017

4929

6022

5322

5108

5473

5274

5285

5308

5044

9000

6451

4498

4191

9000

5896

833

588

939

787

Advances in Agriculture 7

(a)Con

tinued

Sr

No

Genotypes

Germination(

)Ra

teof

emergencelowast

STIlowast

()

SSTIlowast(

)Num

bero

fleavesseedlin

gE 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

2877245times96AC

-93

5472

4705

622154

66

5135

5197

5006

5113

5247

4843

9000

6363

4708

3784

9000

5831

795

662

1058

838

2977245timesTo

go-II

5252

4492

5889

5211

5188

4861

5074

5041

4885

4263

9000

6049

4421

3379

9000

5600

977

751

1243

990

3077245times99HS-18

5740

4099

6872

5570

5249

4898

5258

5135

5628

4616

9000

6415

4863

3014

9000

5626

1045

863

1184

1031

31CV

J-2-5-3-1-3times1305

4364

3944

5700

4669

4974

4517

4650

4714

6097

5579

9000

6892

4594

3842

9000

5812

936

848

1403

1063

32CV

J-2-5-3-1-3times(77371times

BSEC

TCP

-1)4345

3808

6774

4976

5272

4996

5035

5101

6650

5766

9000

7139

4288

3381

9000

5556

800

712

1013

841

33CV

J-2-5-3-1-3times96AC

-93

4862

4197

5743

4934

4884

4980

5058

4974

7477

6760

9000

7745

5957

4709

9000

6555

933

738

1478

1050

34CV

J-2-5-3-1-3timesTo

go-II

4670

4343

6233

5082

4399

4813

4631

4614

5806

6614

9000

7140

4422

4479

9000

5967

963

855

1180

999

35CV

J-2-5-3-1-3times99HS-18

5493

5080

7273

5949

5276

4984

5162

5141

7322

6590

9000

7637

5534

4766

9000

6433

998

807

1338

1047

361305times(77371times

BSEC

TCP

-1)

4613

4539

5717

4956

4986

4955

5080

5007

5137

4870

9000

6336

4210

3936

9000

5715

863

630

913

802

371305times96AC

-93

5473

5177

6830

5827

5224

4779

4919

4974

7117

6965

9000

7694

5640

5220

9000

6620

875

694

1476

1015

381305timesTo

go-II

4230

3420

5262

4304

4309

456144

45

4438

5781

4373

9000

6385

4575

2887

9000

5487

637

566

794

666

391305times99HS-18

5433

4507

6947

5629

5225

4753

4991

4989

7287

6968

9000

7752

5576

4479

9000

6352

1115

818

1410

1114

40(77371times

BSEC

TCP

-1)times

96AC

-93

5595

4429

7461

5828

5216

4955

5121

5097

7087

6368

9000

7485

5432

4038

9000

6157

955

753

1462

1057

41(77371times

BSEC

TCP

-1)times

Togo-II

5252

4728

6106

5362

5492

4808

5132

5144

8025

7505

9000

8177

6310

5413

9000

6908

859

693

990

848

42(77371times

BSEC

TCP

-1)times

99HS-18

5595

4298

6295

5396

4729

4887

5031

4883

7366

6945

9000

7770

6312

4556

9000

6623

903

622

1125

883

4396AC

-93timesTo

go-II

5017

3977

6486

5160

5019

5195

5237

5150

5922

5486

9000

6803

4689

3484

9000

5724

1007

728

1167

967

4496AC

-93times99HS-18

5595

4812

6924

5777

5036

5385

5205

5209

7411

5378

9000

7263

5873

3979

9000

6284

1013

652

1334

1000

45To

go-IItimes

99HS-18

4670

4497

7293

5487

4718

4319

4796

4611

5688

5431

9000

6706

3980

3665

9000

5548

939

653

1462

1018

Mean

5043

4482

6271

5265

4792

498149

23

4899

6377

584190

00

7072

5079

4190

9000

6090

898

701

1168

922

SE097

106

161

mdash213

191

190

mdash19

818

7mdash

mdash14

914

6mdash

mdash036

047

068

mdashCD

272

296

453

mdash597

537

534

mdash555

525

mdashmdash

421

409

mdashmdash

103

133

192

mdashlowastTransfo

rmed

data

(b)

Sr

No

Genotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

908

785

2245

1313

859

1537

1924

1440

154

143

160

152

6267

4800

3833

4967

212

227

249

229

15644

15458

13267

1479

02

H7729-2

1125

1175

1789

1363

317

2400

2811

1843

093

191

252

179

6700

5333

5100

5711

185

210

234

210

18850

18565

15389

17601

3G73-107

1166

1475

1966

1536

943

1744

4945

2544

143

129

366

213

6700

5433

5300

5811

218

219

238

225

15009

1496

513503

14492

477245

1438

1373

2085

1632

607

1863

5957

2809

117

180

509

269

6100

4800

5100

5333

232

221

253

235

14036

13802

11742

1319

35

CVJ-2-5-3-1-3

1214

1437

1807

1486

1163

1610

4166

2313

171

141

407

240

6300

4933

4600

5278

239

227

270

245

1994

317261

1497

017391

61305

858

1137

1737

1244

540

967

4861

2123

125

088

417

210

6533

5033

4800

5456

244

213

257

238

15527

15405

12274

14402

8 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

777371timesBS

ECT

CP-1

1377

1487

1892

1585

928

2660

3330

2306

150

258

301

236

5733

4533

4700

4989

242

220

293

252

1513

814550

1315

014279

896AC

-93

1303

1259

1852

1471

1056

1445

5972

2825

185

147

586

306

5500

4833

3833

4722

256

304

318

293

15577

14032

10000

13203

9To

go-II

1056

1220

2272

1516

924

1466

9113

3834

146

129

883

386

6167

4533

4600

5100

185

149

124

153

14578

12073

10033

12228

1099HS-18

1277

1075

1808

1387

1241

2833

7070

3715

228

226

615

356

6100

5000

4167

5089

243

173

324

247

12627

10355

1017

311052

1H77833-2times

H7729-2

1432

1498

2233

1721

1350

4405

8373

4709

159

499

782

480

5767

4433

3800

4667309

290

248

282

21010

18071

15847

18309

2H77833-2times

G73-107

1675

1635

2205

1838

1236

3211

5789

3412

134

313

547

331

5833

4767

3833

4811

262

295

236

265

21253

20449

15808

1917

0

3H77833-2times

77245

1577

1522

2263

1787

1058

6933

8719

5570

255

553

782

530

5267

4633

4600

4833

273

201

265

246

2074

119511

15208

18487

4H77833-2times

CVJ-2-5-3-1-3

1791

1757

2165

1904

20218030

5276

5109

354

779

477

537

5533

4233

3767

4511

165

330

351

282

21827

20072

17110

19670

5H77833-2times1305

1700

1842

28412128

1427

6228

9134

5596

570

579

813

654

5800

4267

3333

4467340

426

451

406

19816

19444

16037

18432

6H77833-2times

(77371times

BSEC

TCP

-1)

1453

1515

3120

2029

789

465510428

5291

211

408

858

492

5233

4167

3267

4222

203

283

267

251

20677

1913

816470

1876

2

7H77833-2times

96AC

-93

1835

1783

2427

2015

2532

6649

1610

684

29

350

650

1473

824

5467

4233

3700

4467243

239

256

246

22278

20434

15735

19482

8H77833-2times

Togo-II

1564

15632500

1876

1882

4772

11660

6105

296

469

972

579

5667

4533

440048

67

169

260

211

213

2412

82375

619389

22424

9H77833-2times

99HS-18

159318002049

1814

1571

480214024

6799

203

544

1547

765

6033

460040

0048

78

261

225

256

247

23466

21081

1793

320

827

10H7729-2times

G73-107

14951568

2075

1713

959

5157

8517

4877

218

382

666

422

6133

5067

4833

5344

300

273

239

271

23651

2191

717083

20884

11H7729-2times77245

1557

1537

2613

1902

1851

5756

5729

4445366

562

524

484

5633

4800

4633

5022

133

233

297

221

1979

219914

15250

18319

12H7729-2times

CVJ-2-5-3-1-3

18442033

28932257

1651

806114885

8199

264

597

1054

638

6333

4600

4233

5056

170

211

217

199

24629

23339

1970

022556

13H7729-2times1305

1325

1677309920

34

1756

724314048

7682

318

635

1232

728

630046

67

3633

4867298

261

361

307

21666

19371

17422

19486

14H7729-2times(77371

timesBS

ECTCP

-1)

1958

1887

3105

2317

1118

4675

14411

6734

242

457

1318

672

5900

4500

3800

4733

164

209

240

204

23661

1995

419250

20955

15H7729-2times

96AC

-93

1512

1717

2779

2002266844

08

11393

6156

356

500

1211

689

5633

4533

4200

4789

221

195

282

232

2194

921635

17213

20266

Advances in Agriculture 9

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

16H7729-2times

Togo-II

1519

1738

2159

1805

2437

9867

1616

294

89

361

961

1527

950

6167

4700

4200

5022

234

272

230

246

26338

2414

520553

23678

17H7729-2times

99HS-18

1938

1787

3151

2292

1574

6218

11318

6370

290

606

956

617

6067

4767

406749

67

172

210

199

194

24689

21854

1778

921444

18G73-107times77245

1856

1813

2210

1960

2435

8550

7828

6271338

633

612

528

5500

4633

440048

44

199

255

307

254

1993

619277

16095

18436

19G73-107times

CVJ-2-5-3-1-3

1517

1769

2407

1897

1556

7728

9136

6140

239

876

917

677

5900

4300

3400

4533

233

266

315

272

23418

22333

19317

21689

20G73-107times1305

1217

1507

2832

1852

1777

5189

1114

960

38

289

588

1197

691

6733

5000

4233

5322

286

210

312

269

2078

81992

817067

19261

21G73-107times(77371

timesBS

ECTCP

-1)

18551946

2730

2177

1801

3482

10415

5233

239

277

753

423

5500

4467

3433

4467243

197

273

238

23817

20263

17425

20502

22G73-107times

96AC

-93

1718

1840

28612140

1345

9333

14669

8449

297

845

1278

807

5600

5033

4633

5089

270

243

300

271

28537

21461

16875

22291

23G73-107timesTo

go-II

168018083087

2192

1112

4678

9319

5036

186

473

985

548

6033

4767

4200

5000

210

209

231

217

24864

24609

21625

23699

24G73-107times99HS-18

1398

1675

3370

2148

2753

8672

11918

7781

362

691

981

678

566746

00

3500

4589

170

247

270

229

24677

20567

17855

21033

2577245times

CVJ-2-5-3-1-3

1500

1728

2203

1810

2282

4679

11492

6151

328

424

1116

623

6000

4333

420048

44300

231

370

300

21257

20497

1772

519826

2677245times1305

143815772080

1698

949

10439

12053

7814

121

915

992

676

5767

4800

3500

4689

168246

316

244

1978

91779

015517

17699

2777245times(77371times

BSEC

TCP

-1)

150516972846

2016

3222

8518

12621

8120

469

827

1141

812

5400

4367

3833

4533

196

225

242

221

2092

819485

1719

219202

2877245times96AC

-93

1653

1797

2117

1856

1934

6056

11543

6511

352

563

911

608

5333

4267

3833

4478

186

169

198

184

22003

19054

1676

719275

2977245timesTo

go-II

1723

1718

3163

2201

1749

8250

15443

8481229

806

1412

816

5967

4700

4067

4911

174

245

298

239

22075

23503

19875

21818

3077245times99HS-18

2016

1970

28542280

3217

13027

3531

6591395

965

285

549

5733

4467

3833

4678

148

228

209

195

2212

721462

1690

020

163

31CV

J-2-5-3-1-3times

1305

1358

1422

2179

1653

1254

2803

1019

44750

178

254

801

411

6033

4467

3367

4622

312

193

330

278

22269

19547

17250

19689

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

1335

1742

2400

1826

922

5226

12806

6318

236

458

1054

583

5633

4433

3600

4556

321

315

259

298

2210

419524

17087

19572

33CV

J-2-5-3-1-3times

96AC

-93

1552

16803260

2164

166710094

11941

7901250

974

1115

779

5533

4533

446748

44

170

202

317

230

2392

123317

19333

2219

0

34CV

J-2-5-3-1-3times

Togo-II

142216072638

1889

1012

4163

11061

5412

261

377

891

510

6167

4967

4533

5222

207

225

325

252

23590

22860

20278

22243

35CV

J-2-5-3-1-3times

99HS-18

1435

1600

2700

1912

3056

3237

7089

4461449

301

658

469

5700

44673800

4656

148

178

225

183

22444

20240

1696

719884

361305times(77371times

BSEC

TCP

-1)

1499

1767

2058

1774

1882

4665

7920

4822

356

426

724

502

566742673800

4578

271

242

305

273

22011

19295

16417

19241

371305times96AC

-93

16781605

2757

2014

1516

10037

11960

7838

311

11381284

911

560040

673400

4356

182

276

251

236

2396

519625

15800

1979

738

1305timesTo

go-II

1177

16802249

1702

902

4676

9388

4989

241

456

795

497

6533

5533

4733

5600

214

266

296

259

21894

20217

19222

20444

391305times99HS-18

1774

1767

2766

2102

2145

4628

10618

5797

301

447

991

580

5933

4500

3733

4722

128

142

180

150

2275

719413

16967

1971

2

40(77371times

BSEC

TCP

-1)times

96AC

-93

1748

1795

31712238

1537

12586

14379

9501

211

1104

1183

833

546744

00346744

44

127

201

226

185

23294

21089

17858

2074

7

10 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(

days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

41(77371times

BSEC

TCP

-1)times

Togo-II

19652007

2653

2208

2136

5601

9503

5747

315

635

1001

650

5533

4833

410048

22

213

241

286

247

22662

22094

17667

20808

42(77371times

BSEC

TCP

-1)times

99HS-18

15011687

2705

1964

1935

7253

11806

6998

314

704

1067

695

5467

4433

5133

5011

177

167

175

173

22305

19277

16392

19325

4396AC

-93timesTo

go-II1759

1618

2123

1834

1539

10347

12212

8033

325

907

1090

774

5900

450039674789

214

269

339

274

23252

21554

16875

20560

4496AC

-93times

99HS-18

1353

1618

2925

1966

3532

7223

11950

7569

440

654

1024

706

5600

4900

4533

5011

149

175

206

177

24494

20698

18111

2110

145

Togo-IItimes

99HS-18

1117

1278

2582

1659

3448

6764

11910

7374

477

767

1207

817

6000

5133

4333

5156

183

203

216

201

17683

15541

14333

15853

Mean

1513

1618

2492

1874

1656

5773

9854

5761

272

538

885

565

586846

534116

4879

218

233

268

240

21297

19547

16529

1912

4SE

101

073

098

mdash079

152

317

mdash014

014

028

mdash351

365

428

mdash036

039

046

mdash985

1173

1211

mdashCD

284

205

276

mdash221

427

890

mdash038

040

079

mdash12

513

015

2mdash

013

014

016

mdash351

418

431

mdash

(c)

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

1642

1667

1744

1684

1504

1349

1526

1460

2783

2405

2634

2607

836

745

908

830

4287

3754

4160

4067

2H7729-2

1693

1817

1950

1820

1012

859

1016

962

3433

2809

2591

2944

649

597

651

632

4445

3668

3607

3906

3G73-107

2000

2028

2077

2035

2280

1966

2312

2186

4695

4023

4229

4316

1281

1170

1463

1305

6975

5989

6541

6502

477245

1523

1630

1728

1627

1796

2489

2702

2329

5423

4851

4498

4924

748

1464

1743

1319

7219

7341

7200

7253

5CV

J-2-5-3-1-3

2282

2692

2787

2587

2411

2643

2901

2652

5857

4836

5024

5239

1206

1335

1769

1437

8268

7479

7925

7891

61305

1851

1951

2054

1952

2026

1763

1908

1899

4657

4414

4463

4511

1468

1336

1539

1448

6683

6177

6371

6410

777371timesBS

ECTCP

-11629

1645

1733

1669

1244

1692

1884

1607

4925

4183

4435

4515

625

1092

1483

1067

6169

5875

6319

6121

896AC

-93

1379

1519

1600

1499

2066

2942

3088

2698

4501

4095

4229

4275

984

1565

1678

1409

6567

7037

7316

6973

9To

go-II

1904

2057

2103

2021

2167

1844

2358

2123

4520

3446

3904

3957

1298

1138

1502

1313

6687

5290

6262

6080

1099HS-18

1475

1485

1633

1531

3180

2904

3382

3155

5633

4139

5205

4992

1656

1536

1879

1690

8813

7043

8587

8147

1H77833-2timesH7729-2

2010

2046

2133

2063

3645

3075

4377

3699

7675

6540

7199

7138

2014

1708

2880

2201

11320

9615

11576

10837

2H77833-2timesG73-107

2066

2101

2122

2096

5002

4053

4601

4552

7882

7430

6963

7425

3146

2565

2808

2840

12884

11482

11564

11977

3H77833-2times77245

1764

2048

2083

1965

3769

4497

4559

4275

8681

8515

8475

8557

2284

3356

3377

3006

12450

13012

13034

12832

4H77833-2times

CVJ-2-5-3-1-3

2205

2383

2733

2441

3693

5655

5220

4856

11656

10287

1010

510683

2198

4488

4176

3621

15348

1594

215325

15538

5H77833-2times1305

2209

2423

2491

2374

6008

5842

6933

6261

15238

14311

13889

14479

3758

4057

5591

4469

21246

2015

320822

2074

0

6H77833-2times(77371times

BSEC

TCP

-1)

1789

1823

1992

1868

3593

4288

4903

4262

8922

6522

7429

7625

2178

3224

3070

2824

12515

10811

12333

11886

7H77833-2times96AC

-93

1739

1960

2011

1903

4557

4350

4818

4575

7916

6068

7290

7092

2411

2529

3191

2710

12474

10419

1210

811667

8H77833-2timesTo

go-II

1927

2389

2406

2241

4185

5828

6215

5409

12319

10279

11023

11207

2433

4254

4631

3773

16504

1610

717238

16616

9H77833-2times99HS-18

1941

2245

2330

2172

4227

4139

4529

4298

9211

8120

7969

8433

3154

3184

3253

3197

13438

12259

12498

1273

210

H7729-2timesG73-107

1968

2109

2158

2079

3725

3042

3488

3418

7313

5956

6174

6481

2822

2001

2357

2393

11038

8998

9662

9899

Advances in Agriculture 11

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

11H7729-2times77245

1847

2048

2200

2032

2467

3759

5466

3898

7672

7449

8921

8014

1356

2350

3796

2500

1013

911208

14387

11911

12H7729-2times

CVJ-2-5-3-1-3

2218

2585

2767

2523

3674

4372

4483

4176

10053

9467

8664

9395

2417

3312

3345

3025

1372

813839

1314

713571

13H7729-2times1305

2169

2338

2547

2352

6728

6166

6792

6562

13333

11662

13301

1276

54128

4501

5660

4763

20061

17828

20093

19327

14H7729-2times(77371times

BSEC

TCP

-1)

1925

2095

2127

2049

3530

4165

4649

4115

9638

6917

7698

8084

1681

2833

3150

2555

1316

8110

82

12347

1219

915

H7729-2times96AC

-93

2012

2070

2180

2087

4955

4447

4596

4666

8738

6882

7762

7794

3754

3558

3181

3498

13693

11329

12359

12460

16H7729-2timesTo

go-II

2138

2428

2563

2377

4949

4660

5118

4909

8549

7243

7191

7661

3586

3353

3028

3322

13498

11903

12310

12570

17H7729-2times99HS-18

1899

1933

2073

1969

4046

4527

4988

4520

8492

7895

8144

8177

2561

3378

3553

3164

12539

12422

1313

212698

18G73-107times77245

1840

2253

2437

2177

2580

4776

5915

4424

11673

10608

10459

10913

1358

3163

4286

2936

14253

15385

16374

15337

19G73-107times

CVJ-2-5-3-1-3

2324

2537

2691

2517

4958

5030

5599

5196

9524

9395

1114

610022

3241

3645

4444

3776

14483

14425

1674

515217

20G73-107times1305

2228

2433

2583

2415

6340

5794

6373

6169

12490

1015

8119

61

11536

4199

4199

5018

4472

18830

1595

218334

1770

6

21G73-107times(77371times

BSEC

TCP

-1)

1943

2128

2220

2097

3936

3980

5770

4562

11697

9858

10303

10619

1874

2369

4181

2808

15633

13839

16073

1518

222

G73-107times96AC

-93

2118

2218

2317

2217

4270

5843

6201

5438

10319

9872

1015

910117

2703

4460

4379

3847

14589

15715

16359

15555

23G73-107timesTo

go-II

2237

2365

2733

2445

5736

5175

6822

5911

12098

10808

1299

611967

3984

3670

5372

4342

17835

1598

219818

17878

24G73-107times99HS-18

2025

2081

2322

2143

3558

4229

6612

4800

12350

9685

11516

1118

42196

2782

5009

3329

1590

813914

1812

915983

2577245timesCV

J-2-5-3-1-3

2109

2360

2675

2381

6760

6558

7299

6872

15225

11861

1416

21374

94225

4752

5839

4939

2198

518418

21461

20621

2677245times1305

1919

2100

2197

2072

5700

5142

5347

5397

11687

10565

10737

10996

4318

3956

4244

4173

17386

1570

816085

16393

2777245times(77371times

BSEC

TCP

-1)

1851

1935

2020

1936

2577

2460

2838

2625

6989

4778

4636

5468

1631

1662

1999

1764

9566

7238

7474

8093

2877245times96AC

-93

1729

1780

2133

1881

5541

5058

5748

5449

11952

8174

9526

9884

3646

3587

4071

3768

17493

13232

15274

15333

2977245timesTo

go-II

2300

2508

2658

2489

3067

5431

5963

4820

11737

11021

1216

411641

1598

4053

4770

3474

14805

16452

1812

616461

3077245times99HS-18

1982

2019

2223

2075

3846

3119

4225

3730

7493

6720

6476

6896

3205

2578

2643

2809

11339

9839

1070

110626

12 Advances in Agriculture

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

31CV

J-2-5-3-1-3times1305

2406

2553

3067

2675

4178

4796

6281

5085

13822

11425

12252

12500

2472

3241

4907

3540

18000

16222

18532

17585

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

2142

2173

2238

2184

4522

5235

5640

5132

11673

9658

10248

10526

2614

3766

4178

3519

1619

514893

15888

15659

33CV

J-2-5-3-1-3times

96AC

-93

2350

2477

2600

2476

4676

4280

6090

5015

11975

9832

10868

10891

3097

2931

4445

3491

16651

14111

1695

815907

34CV

J-2-5-3-1-3times

Togo-II

2093

2666

2989

2583

4058

3727

5190

4325

12588

10717

11217

11507

2742

2570

4185

3166

16645

14443

16407

15832

35CV

J-2-5-3-1-3times

99HS-18

2028

2311

2386

2242

3287

2513

5356

3719

10055

9733

1036

410051

2004

1581

4251

2612

13342

12246

1572

01376

9

361305times(77371times

BSEC

TCP

-1)

2005

2173

2210

2129

3569

4692

5140

4467

11652

9826

10041

10507

2005

3665

4179

3283

15221

14518

1518

214974

371305times96AC

-93

1822

2003

2047

1957

5115

4023

4813

4650

9965

7675

7571

8404

3456

2647

3155

3086

15080

11698

12384

13054

381305timesTo

go-II

2545

2583

2644

2591

6885

7283

6795

6987

12265

11410

12583

12086

4500

6120

5350

5323

1915

018693

19378

19074

391305times99HS-18

2070

2408

2593

2357

5219

4332

4800

4784

1092

97677

8968

9192

3924

3332

3840

3699

1614

912009

1376

913976

40(77371times

BSEC

TCP

-1)

times96AC

-93

2056

2115

2157

2109

3158

4463

4717

4113

9166

7540

8100

8268

1880

3542

3347

2923

12324

12003

12817

12381

41(77371times

BSEC

TCP

-1)

timesTo

go-II

1868

2253

2450

2190

4422

6458

7038

5973

1415

312369

1379

713439

2291

4646

5586

4174

18575

18827

20835

19412

42(77371times

BSEC

TCP

-1)

times99HS-18

1995

2026

2209

2077

2720

2909

3543

3057

7392

4692

5023

5702

1766

2187

2147

2033

10112

7601

8566

8760

4396AC

-93timesTo

go-II

1953

2027

2511

2164

5972

4391

7126

5830

15537

1392

714432

14632

3780

2538

5655

3991

21510

18318

21558

20462

4496AC

-93times99HS-18

1952

2021

2242

2072

3465

2758

4496

3573

5959

5186

6094

5746

2707

2154

2409

2423

9424

7944

10590

9319

45To

go-IItimes

99HS-18

1932

2008

2658

2199

5381

4995

5436

5270

10299

8876

9244

9473

3986

3842

3668

3832

15680

13871

14680

1474

3Mean

1982

2146

2301

2143

3963

4123

4836

4307

9498

8087

8663

8749

2510

2914

3513

2979

13460

12210

13499

13056

SE243

198

245

mdash671

501

626

mdash959

750

822

mdash423

349

344

mdash1155

927

1167

mdashCD

087

071

087

mdash239

178

223

mdash342

267

293

mdash15

112

412

2mdash

411

330

416

mdash

Advances in Agriculture 13Ta

ble4Analysis

ofvaria

ncefor

10times10

half-diallelfor

different

charactersin

threee

nviro

nments

(a)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Germination

()

Rateof

emergence

STI()

SSTI

()

Num

bero

fleavesseedling

Seedlin

gheight

(cm)

Freshweight

(g)seedlin

gDry

weight

(g)seedlin

g

E 1

Replicates

2652731

596768

783393

20700

93018674

33764

1033999

002590

Treatm

ents

541097

0255lowastlowast

8181153lowastlowast

30863092lowastlowast

20680900lowastlowast

1100708lowastlowast

2106317lowastlowast

930829527lowastlowast

319767lowastlowast

Parents

95118

163lowastlowast

14289925lowastlowast

59114816lowastlowast

18880254lowastlowast

1084227lowastlowast

1087906lowastlowast

22561719lowastlowast

042162lowastlowast

Hybrid

s44

11423573lowastlowast

3398933lowastlowast

25697199lowastlowast

20633716lowastlowast

472874lowastlowast

1391526lowastlowast

674123518lowastlowast

261839lowastlowast

Parentsv

ersush

ybrid

s1

43693104lowastlowast

163619

916lowastlowast

3896838

3896

2793lowastlowast

28873741lowastlowast

4272

2798lowastlowast

234144289lowastlowast

5367022lowastlowast

Error

108

281184

1356832

1173684

674337

040104

306

462

2015

1619

005535

E 2

Replicates

2583819

3707389lowast

1596635

198738

034925

448213

899529

007177

Treatm

ents

549997443lowastlowast

2825177lowastlowast

35378177lowastlowast

19074306lowastlowast

665284lowastlowast

1764

081lowastlowast

257694570lowastlowast

2177519lowastlowast

Parents

96507784lowastlowast

3718

879lowastlowast

69866289lowastlowast

1798

5404lowastlowast

705379lowastlowast

1408518lowastlowast

10612519lowastlowast

077429lowastlowast

Hybrid

s44

10569731lowastlowast

2127302lowastlowast

2910

2537lowastlowast

19429240lowastlowast

329256lowastlowast

7000

03lowastlowast

186026552lowastlowast

1483428lowastlowast

Parentsv

ersush

ybrid

s1

16223710lowastlowast

25488361lowastlowast

1113

326

13257362lowastlowast

15089673lowastlowast

5178

3569lowastlowast

5634825791lowastlowast

51618348lowastlowast

Error

108

334760

1097039

1049505

635570

06764

116

0576

694613

006206

E 3

Replicates

2894398

4808815lowast

mdashmdash

051076

095989

3768421

030220

Treatm

ents

541261560

0lowastlowast

4842216lowastlowast

mdashmdash

2811077lowastlowast

5947316lowastlowast

3870844

50lowastlowast

3445076lowastlowast

Parents

97135

735lowastlowast

1097

6388lowastlowast

mdashmdash

1468699lowastlowast

1112

764lowastlowast

136081650lowastlowast

1307836lowastlowast

Hybrid

s44

13217740lowastlowast

1379360

mdashmdash

15540

71lowastlowast

4581632lowastlowast

252084767lowastlowast

2378032lowastlowast

Parentsv

ersush

ybrid

s1

35440

180lowastlowast

102000335lowastlowast

mdashmdash

70200767lowastlowast

109548362lowastlowast

8586095705lowastlowast

69630188lowastlowast

Error

108

782907

1085444

mdashmdash

139771

291325

3019

169

02400

4(b)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Panicle

emergence

Effectiv

etillersplant

Planth

eight

Earlength

Earw

eightp

lant

Dry

fodd

eryieldplant

Grain

yieldplant

Totalbiological

yieldplant

E 1

Replicates

231860

61lowastlowast

416348lowastlowast

1672

9472lowast

1706

411077192

9634539

392777

115544

59Treatm

ents

544152

368lowastlowast

085657lowastlowast

326706089lowastlowast

1602572lowastlowast

6177

0406lowastlowast

304113

204lowastlowast

34285030lowastlowast

575617

582lowastlowast

Parents

94511481lowastlowast

018538lowastlowast

140807476lowastlowast

2240093lowastlowast

11653

959lowastlowast

27014155lowastlowast

3858650lowastlowast

61321919lowastlowast

Hybrid

s44

3201347lowastlowast

100797lowastlowast

110395657lowastlowast

1010

497lowastlowast

40296671lowastlowast

171271411lowastlowast

2413

6880lowastlowast

302999650lowastlowast

Parentsv

ersush

ybrid

s1

4276

5286lowastlowast

02360

9lowast11517452

616lowastlowast

2191

6167lowastlowast

1457662749lowastlowast

8643043524lowastlowast

7546

41053lowastlowast

17199467546lowastlowast

Error

108

468159

004907

3692099

224610

17114

303498126

682350

5075268

E 2

Replicates

2656364

002556

61575565lowastlowast

828842lowastlowast

589094

2268506

260877

1507116

Treatm

ents

542999618lowastlowast

069822lowastlowast

268998723lowastlowast

2436886lowastlowast

60592784lowastlowast

247259877lowastlowast

4192

0073lowastlowast

513483489lowastlowast

Parents

926300

00lowastlowast

048079lowastlowast

165555

405lowastlowast

3871874lowastlowast

1412

2393lowastlowast

19543682lowastlowast

3132

426lowastlowast

57230706lowastlowast

Hybrid

s44

2533

165lowastlowast

07344

9lowastlowast

9616

2857lowastlowast

1463359lowastlowast

35495366lowastlowast

154759288lowastlowast

26262465lowastlowast

293492304lowastlowast

Parentsv

ersush

ybrid

s1

26850135lowastlowast

105929lowastlowast

880476

6697lowastlowast

32357161lowastlowast

1583112746lowastlowast

6366731560lowastlowast

107994

3646lowastlowast

14299370699lowastlowast

Error

108

507598

005829

5234122

149406

955133

2142411

462311

3273886

E 3

Replicates

2029697

021161

9459847

143746

985141

46800

6492

6334

3345160

Treatm

ents

548045499lowastlowast

094511lowastlowast

192420108lowastlowast

3279225lowastlowast

72049211lowastlowast

298953

021lowastlowast

55061308lowastlowast

651732994lowastlowast

Parents

98010

741lowastlowast

094166lowastlowast

116037343lowastlowast

3702185lowastlowast

16305190lowastlowast

2323644

0lowastlowast

4553

893lowastlowast

71484982lowastlowast

Hybrid

s44

6254377lowastlowast

095514lowastlowast

7378

1513lowastlowast

2188348lowastlowast

31813193lowastlowast

190263916lowastlowast

31572179lowastlowast

368689934lowastlowast

Parentsv

ersush

ybrid

s1

8716

7710lowastlowast

053496lowast

609996

3199lowastlowast

47471212lowastlowast

2344

130203lowastlowast

756272

2861lowastlowast

154314

9707lowastlowast

18327859

719lowastlowast

Error

108

696981

007883

5578505

228608

1491014

2574

223

449588

5187705

lowast

Sign

ificant

at119875=005

lowastlowastSign

ificant

at119875=001

14 Advances in Agriculture

Table 5 Mean performance of the genotypes for membrane thermostability in two environments

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594

Advances in Agriculture 15

Table 5 Continued

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434

Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash

lowastValues are transformed values

0

6

12

18

24

30

36

42

48

54

minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity

G73-10796AC-93CVJ-2-5-3-1-3

Togo-II99HS-18

H77833-2H7729-2772451305

H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305

1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305

H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305

77245 times 1305

77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305

77371 times BSECT CP-1

H77833-2 times G73-107

Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits

moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling

survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The

16 Advances in Agriculture

Table6Po

oled

correla

tioncoeffi

cientamon

gvario

uscharactersin

threee

nviro

nments

Characters

Germination

Rateof

emergence

STI

SSTI

Leaves

seedlin

gSeedlin

gheight

Fresh

weight

Dry

weight

MTS

Panicle

emergence

Effectiv

etillersplant

Plant

height

Earlength

Ear

weightp

lant

Dry

fodd

eryield

plant

Grain

yield

plant

Total

biological

yield

plant

Germination

1000

0357lowastlowast

0353lowastlowast

0590lowastlowast

0230

0373lowastlowast

0293lowast

0260

0276lowastminus0135minus0478lowastlowast

0326lowast

minus0069minus0104

minus0190

minus0145

minus0167

Rateof

emergence

1000

0233

0351lowastlowast

0605lowastlowast

0585lowastlowast

0551lowastlowast

0524lowastlowast

0139minus0593lowastlowast

0032

0517lowastlowast

0217

0388lowastlowast

0361lowastlowast

0348lowastlowast

0377lowastlowast

STI

1000

0837lowastlowast

0241

0129

0081

004

80890lowastlowast

minus0215

minus0211

0030minus0007minus0043

minus0101

minus0111

minus0084

SSTI

1000

0233

0264

0183

0140

0745lowastlowast

minus0247minus0268lowast

0184minus0017minus006

6minus0129

minus0144

minus0112

Num

bero

fleaves

1000

0683lowastlowast

0633lowastlowast

060

4lowastlowast

0176minus0474lowastlowast

0109

0576lowastlowast

0496lowastlowast

064

1lowastlowast

0607lowastlowast

0630lowastlowast

0630lowastlowast

Seedlin

gheight

1000

0703lowastlowast

0663lowastlowast

0031minus0493lowastlowast

minus0115

0735lowastlowast

0360lowastlowast

0526lowastlowast

0520lowastlowast

0515lowastlowast

0532lowastlowast

Freshweight

1000

0957lowastlowast

minus0016

0425lowastlowast

minus0082

0661lowastlowast

0351lowastlowast

0581lowastlowast

0496lowastlowast

0576lowastlowast

0533lowastlowast

Dry

weight

1000minus0033minus0428lowastlowast

minus0016

0634lowastlowast

0354lowastlowast

0620lowastlowast

0509lowastlowast

0614lowastlowast

0554lowastlowast

MTS

1000minus040

minus0177minus0037

0015

minus0074

minus0138

minus0131

minus0120

Panicle

emergence

1000

minus0150minus0269lowast

0022minus0329lowast

minus0337lowast

minus0280lowast

minus0341lowast

Effectiv

etillersplant

1000

minus0012

0170

0366lowastlowast

040

5lowastlowast

0323lowast

0401lowastlowast

Planth

eight

1000

0650lowastlowast

0603lowastlowast

0596lowastlowast

0619lowastlowast

0610lowastlowast

Earlength

1000

0608lowastlowast

0671lowastlowast

064

8lowastlowast

066

4lowastlowast

Earw

eightp

lant

1000

0910lowastlowast

0984lowastlowast

0957lowastlowast

Dry

fodd

eryield

plant

1000

0907lowastlowast

0991lowastlowast

Grain

yield

plant

1000

0950lowastlowast

Totalbiological

yield

plant

1000

lowastSign

ificant

at119875=005lowastlowastSign

ificant

at119875=001

Advances in Agriculture 17

faster germination and early vigour in seedling could act asphysical indicators for heat tolerance

Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits

Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics

Conflict of Interests

The authors declare that there is no conflict of interestsregarding the publication of this paper

References

[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981

[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993

[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006

[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993

[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013

[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007

[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965

[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011

[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001

[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009

[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962

[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967

[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963

[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001

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GenomicsInternational Journal of

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Page 6: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

6 Advances in Agriculture

Table3Perfo

rmance

ofthep

arentsandthec

rosses

ford

ifferentcharacters

(a)

Sr

No

Genotypes

Germination(

)Ra

teof

emergencelowast

STIlowast

()

SSTIlowast(

)Num

bero

fleavesseedlin

gE 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

3941

3737

6164

4614

4037

4109

4279

4142

7830

7387

9000

8072

4498

4133

9000

5877

661

502

800

654

2H7729-2

5513

4726

6092

5443

2815

4980

3258

3684

4835

4495

9000

6110

4441

3602

9000

5681

363

294

427

361

3G73-107

5037

4826

5918

5260

4534

4132

4481

4382

6187

5413

9000

6866

5213

4460

9000

6224

878

710

935

841

477245

4747

4624

5565

4978

3625

4604

4047

4092

4385

3580

9000

5655

3803

3040

9000

5281

377

292

437

369

5CV

J-2-5-3-1-3

4824

4832

6897

5518

5044

4907

4960

4970

7193

6813

9000

7669

4959

4785

9000

6248

802

580

923

768

61305

4326

3891

5404

4540

3376

5056

3573

4002

3903

3281

9000

5395

3211

2452

9000

4888

350

284

414

349

777371timesBS

ECTCP

-14747

4502

6320

5190

4934

5075

5065

5025

6952

6706

9000

7552

5056

4671

9000

6242

621

542

700

621

896AC

-93

4651

3818

5722

4730

4312

4915

4753

4660

6660

6001

9000

7220

5212

3920

9000

6044

701

590

899

730

9To

go-II

4613

3937

5276

4609

4245

4737

4797

4593

7198

6861

9000

7686

5980

4767

9000

6582

681

629

853

721

1099HS-18

4575

3823

6247

4882

4317

4659

4746

4574

7594

7319

9000

7971

5154

4172

9000

6109

745

554

915

738

1H77833-2timesH7729-2

5787

5295

6733

5938

4964

5015

4989

4989

6803

6504

9000

7436

5808

5134

9000

6647

711

585

780

692

2H77833-2timesG73-107

5472

4417

6405

5431

5448

5422

5239

5370

7231

6769

9000

7667

5980

4556

9000

6512

1101

705

1340

1049

3H77833-2times77245

4076

3707

5504

4429

5217

5446

5238

5300

6627

6224

9000

7284

4690

4037

9000

5909

740

525

965

744

4H77833-2timesCV

J-2-5-3-1-3

4843

4300

5856

5000

4862

4956

4913

4911

7061

6519

9000

7527

5575

4632

9000

6403

998

885

1258

1047

5H77833-2times1305

3296

2780

4521

3533

5076

4907

5238

5074

3732

3265

9000

5332

2765

2050

9000

4605

1192

934

1600

1242

6H77833-2times(77371times

BSEC

TCP

-1)5636

5539

6258

5811

4489

5219

5015

4908

6981

6482

9000

7488

6146

5680

9000

6942

898

726

1525

1050

7H77833-2times96AC

-93

5720

5515

6335

5857

4800

5186

5075

5021

7833

7786

9000

8206

6603

6256

9000

7287

1107

873

1193

1058

8H77833-2timesTo

go-II

4862

3453

5709

4675

5036

4829

4911

4925

6190

5500

9000

6897

5214

3339

9000

5851

979

670

1107

919

9H77833-2times99HS-18

4575

4418

5447

4813

5041

5074

5006

5040

5800

5073

9000

6625

4846

4133

9000

5993

978

733

1135

949

10H7729-2timesG73-107

5615

4962

6273

5617

5023

5535

5165

5241

6143

5501

9000

6881

5540

4441

9000

6327

1000

720

1363

1028

11H7729-2times77245

5472

5164

6281

5639

4745

5031

5028

4935

5572

5175

9000

6582

4901

4345

9000

6082

880

620

897

799

12H7729-2timesCV

J-2-5-3-1-3

5494

5124

6536

5718

4804

5316

4957

5026

7596

8076

9000

8224

6093

5680

9000

6924

981

932

1451

1121

13H7729-2times1305

3804

3826

5511

4380

3596

4724

4387

4236

6362

7075

9000

7479

4230

4517

9000

5916

926

725

1497

1049

14H7729-2times(77371times

BSEC

TCP

-1)

6369

4847

7412

6209

4847

5351

5246

5148

6377

6031

9000

7136

5683

4230

9000

6304

977

671

1380

1009

15H7729-2times96AC

-93

5314

4657

6702

5558

4514

5295

5102

4971

5612

4856

9000

6489

4613

3603

9000

5739

846

679

1397

974

16H7729-2timesTo

go-II

5827

5594

6651

6024

5072

5279

5271

5207

6563

5371

9000

6978

5763

4652

9000

6472

873

719

1040

877

17H7729-2times99HS-18

5741

4638

7918

6099

4847

5024

5021

4964

6179

5603

9000

6927

4882

3725

9000

5869

1012

730

1600

1114

18G73-107times77245

5115

4646

6103

5288

4977

5242

5450

5223

7006

6063

9000

7356

5685

4594

9000

6426

1021

790

1292

1034

19G73-107timesCV

J-2-5-3-1-3

5134

4894

6427

5485

4901

5317

5181

5133

6637

6124

9000

7254

5253

4710

9000

6321

1039

863

1254

1052

20G73-107times1305

4881

4561

7597

5680

4717

4791

4775

4761

6704

6380

9000

7361

4556

4115

9000

5890

1140

882

1657

1226

21G73-107times(77371times

BSEC

TCP

-1)

5636

5519

6283

5812

4986

5219

5203

5136

7990

6203

9000

7731

6620

5436

9000

7019

977

703

1395

1025

22G73-107times96AC

-93

5740

4510

6415

5555

5129

4982

5195

5102

6978

5556

9000

7178

6156

4019

9000

6392

1077

860

1264

1067

23G73-107timesTo

go-II

4460

4357

6044

4953

4396

5138

4951

4829

5279

4835

9000

6371

4018

3603

9000

5540

906

881

1189

992

24G73-107times99HS-18

5473

4356

6374

5401

5102

4955

5055

5037

6460

5197

9000

6886

5534

3704

9000

6079

1171

766

1635

1191

2577245timesCV

J-2-5-3-1-3

4766

4666

5921

5117

5170

4918

4900

4996

5928

5859

9000

6929

4785

4613

9000

6133

984

920

1194

1033

2677245times1305

4575

4060

6052

4895

5128

5297

5203

5210

5102

4990

9000

6364

3979

3464

9000

5481

1279

823

1550

1217

2777245times(77371times

BSEC

TCP

-1)

5017

4929

6022

5322

5108

5473

5274

5285

5308

5044

9000

6451

4498

4191

9000

5896

833

588

939

787

Advances in Agriculture 7

(a)Con

tinued

Sr

No

Genotypes

Germination(

)Ra

teof

emergencelowast

STIlowast

()

SSTIlowast(

)Num

bero

fleavesseedlin

gE 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

2877245times96AC

-93

5472

4705

622154

66

5135

5197

5006

5113

5247

4843

9000

6363

4708

3784

9000

5831

795

662

1058

838

2977245timesTo

go-II

5252

4492

5889

5211

5188

4861

5074

5041

4885

4263

9000

6049

4421

3379

9000

5600

977

751

1243

990

3077245times99HS-18

5740

4099

6872

5570

5249

4898

5258

5135

5628

4616

9000

6415

4863

3014

9000

5626

1045

863

1184

1031

31CV

J-2-5-3-1-3times1305

4364

3944

5700

4669

4974

4517

4650

4714

6097

5579

9000

6892

4594

3842

9000

5812

936

848

1403

1063

32CV

J-2-5-3-1-3times(77371times

BSEC

TCP

-1)4345

3808

6774

4976

5272

4996

5035

5101

6650

5766

9000

7139

4288

3381

9000

5556

800

712

1013

841

33CV

J-2-5-3-1-3times96AC

-93

4862

4197

5743

4934

4884

4980

5058

4974

7477

6760

9000

7745

5957

4709

9000

6555

933

738

1478

1050

34CV

J-2-5-3-1-3timesTo

go-II

4670

4343

6233

5082

4399

4813

4631

4614

5806

6614

9000

7140

4422

4479

9000

5967

963

855

1180

999

35CV

J-2-5-3-1-3times99HS-18

5493

5080

7273

5949

5276

4984

5162

5141

7322

6590

9000

7637

5534

4766

9000

6433

998

807

1338

1047

361305times(77371times

BSEC

TCP

-1)

4613

4539

5717

4956

4986

4955

5080

5007

5137

4870

9000

6336

4210

3936

9000

5715

863

630

913

802

371305times96AC

-93

5473

5177

6830

5827

5224

4779

4919

4974

7117

6965

9000

7694

5640

5220

9000

6620

875

694

1476

1015

381305timesTo

go-II

4230

3420

5262

4304

4309

456144

45

4438

5781

4373

9000

6385

4575

2887

9000

5487

637

566

794

666

391305times99HS-18

5433

4507

6947

5629

5225

4753

4991

4989

7287

6968

9000

7752

5576

4479

9000

6352

1115

818

1410

1114

40(77371times

BSEC

TCP

-1)times

96AC

-93

5595

4429

7461

5828

5216

4955

5121

5097

7087

6368

9000

7485

5432

4038

9000

6157

955

753

1462

1057

41(77371times

BSEC

TCP

-1)times

Togo-II

5252

4728

6106

5362

5492

4808

5132

5144

8025

7505

9000

8177

6310

5413

9000

6908

859

693

990

848

42(77371times

BSEC

TCP

-1)times

99HS-18

5595

4298

6295

5396

4729

4887

5031

4883

7366

6945

9000

7770

6312

4556

9000

6623

903

622

1125

883

4396AC

-93timesTo

go-II

5017

3977

6486

5160

5019

5195

5237

5150

5922

5486

9000

6803

4689

3484

9000

5724

1007

728

1167

967

4496AC

-93times99HS-18

5595

4812

6924

5777

5036

5385

5205

5209

7411

5378

9000

7263

5873

3979

9000

6284

1013

652

1334

1000

45To

go-IItimes

99HS-18

4670

4497

7293

5487

4718

4319

4796

4611

5688

5431

9000

6706

3980

3665

9000

5548

939

653

1462

1018

Mean

5043

4482

6271

5265

4792

498149

23

4899

6377

584190

00

7072

5079

4190

9000

6090

898

701

1168

922

SE097

106

161

mdash213

191

190

mdash19

818

7mdash

mdash14

914

6mdash

mdash036

047

068

mdashCD

272

296

453

mdash597

537

534

mdash555

525

mdashmdash

421

409

mdashmdash

103

133

192

mdashlowastTransfo

rmed

data

(b)

Sr

No

Genotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

908

785

2245

1313

859

1537

1924

1440

154

143

160

152

6267

4800

3833

4967

212

227

249

229

15644

15458

13267

1479

02

H7729-2

1125

1175

1789

1363

317

2400

2811

1843

093

191

252

179

6700

5333

5100

5711

185

210

234

210

18850

18565

15389

17601

3G73-107

1166

1475

1966

1536

943

1744

4945

2544

143

129

366

213

6700

5433

5300

5811

218

219

238

225

15009

1496

513503

14492

477245

1438

1373

2085

1632

607

1863

5957

2809

117

180

509

269

6100

4800

5100

5333

232

221

253

235

14036

13802

11742

1319

35

CVJ-2-5-3-1-3

1214

1437

1807

1486

1163

1610

4166

2313

171

141

407

240

6300

4933

4600

5278

239

227

270

245

1994

317261

1497

017391

61305

858

1137

1737

1244

540

967

4861

2123

125

088

417

210

6533

5033

4800

5456

244

213

257

238

15527

15405

12274

14402

8 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

777371timesBS

ECT

CP-1

1377

1487

1892

1585

928

2660

3330

2306

150

258

301

236

5733

4533

4700

4989

242

220

293

252

1513

814550

1315

014279

896AC

-93

1303

1259

1852

1471

1056

1445

5972

2825

185

147

586

306

5500

4833

3833

4722

256

304

318

293

15577

14032

10000

13203

9To

go-II

1056

1220

2272

1516

924

1466

9113

3834

146

129

883

386

6167

4533

4600

5100

185

149

124

153

14578

12073

10033

12228

1099HS-18

1277

1075

1808

1387

1241

2833

7070

3715

228

226

615

356

6100

5000

4167

5089

243

173

324

247

12627

10355

1017

311052

1H77833-2times

H7729-2

1432

1498

2233

1721

1350

4405

8373

4709

159

499

782

480

5767

4433

3800

4667309

290

248

282

21010

18071

15847

18309

2H77833-2times

G73-107

1675

1635

2205

1838

1236

3211

5789

3412

134

313

547

331

5833

4767

3833

4811

262

295

236

265

21253

20449

15808

1917

0

3H77833-2times

77245

1577

1522

2263

1787

1058

6933

8719

5570

255

553

782

530

5267

4633

4600

4833

273

201

265

246

2074

119511

15208

18487

4H77833-2times

CVJ-2-5-3-1-3

1791

1757

2165

1904

20218030

5276

5109

354

779

477

537

5533

4233

3767

4511

165

330

351

282

21827

20072

17110

19670

5H77833-2times1305

1700

1842

28412128

1427

6228

9134

5596

570

579

813

654

5800

4267

3333

4467340

426

451

406

19816

19444

16037

18432

6H77833-2times

(77371times

BSEC

TCP

-1)

1453

1515

3120

2029

789

465510428

5291

211

408

858

492

5233

4167

3267

4222

203

283

267

251

20677

1913

816470

1876

2

7H77833-2times

96AC

-93

1835

1783

2427

2015

2532

6649

1610

684

29

350

650

1473

824

5467

4233

3700

4467243

239

256

246

22278

20434

15735

19482

8H77833-2times

Togo-II

1564

15632500

1876

1882

4772

11660

6105

296

469

972

579

5667

4533

440048

67

169

260

211

213

2412

82375

619389

22424

9H77833-2times

99HS-18

159318002049

1814

1571

480214024

6799

203

544

1547

765

6033

460040

0048

78

261

225

256

247

23466

21081

1793

320

827

10H7729-2times

G73-107

14951568

2075

1713

959

5157

8517

4877

218

382

666

422

6133

5067

4833

5344

300

273

239

271

23651

2191

717083

20884

11H7729-2times77245

1557

1537

2613

1902

1851

5756

5729

4445366

562

524

484

5633

4800

4633

5022

133

233

297

221

1979

219914

15250

18319

12H7729-2times

CVJ-2-5-3-1-3

18442033

28932257

1651

806114885

8199

264

597

1054

638

6333

4600

4233

5056

170

211

217

199

24629

23339

1970

022556

13H7729-2times1305

1325

1677309920

34

1756

724314048

7682

318

635

1232

728

630046

67

3633

4867298

261

361

307

21666

19371

17422

19486

14H7729-2times(77371

timesBS

ECTCP

-1)

1958

1887

3105

2317

1118

4675

14411

6734

242

457

1318

672

5900

4500

3800

4733

164

209

240

204

23661

1995

419250

20955

15H7729-2times

96AC

-93

1512

1717

2779

2002266844

08

11393

6156

356

500

1211

689

5633

4533

4200

4789

221

195

282

232

2194

921635

17213

20266

Advances in Agriculture 9

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

16H7729-2times

Togo-II

1519

1738

2159

1805

2437

9867

1616

294

89

361

961

1527

950

6167

4700

4200

5022

234

272

230

246

26338

2414

520553

23678

17H7729-2times

99HS-18

1938

1787

3151

2292

1574

6218

11318

6370

290

606

956

617

6067

4767

406749

67

172

210

199

194

24689

21854

1778

921444

18G73-107times77245

1856

1813

2210

1960

2435

8550

7828

6271338

633

612

528

5500

4633

440048

44

199

255

307

254

1993

619277

16095

18436

19G73-107times

CVJ-2-5-3-1-3

1517

1769

2407

1897

1556

7728

9136

6140

239

876

917

677

5900

4300

3400

4533

233

266

315

272

23418

22333

19317

21689

20G73-107times1305

1217

1507

2832

1852

1777

5189

1114

960

38

289

588

1197

691

6733

5000

4233

5322

286

210

312

269

2078

81992

817067

19261

21G73-107times(77371

timesBS

ECTCP

-1)

18551946

2730

2177

1801

3482

10415

5233

239

277

753

423

5500

4467

3433

4467243

197

273

238

23817

20263

17425

20502

22G73-107times

96AC

-93

1718

1840

28612140

1345

9333

14669

8449

297

845

1278

807

5600

5033

4633

5089

270

243

300

271

28537

21461

16875

22291

23G73-107timesTo

go-II

168018083087

2192

1112

4678

9319

5036

186

473

985

548

6033

4767

4200

5000

210

209

231

217

24864

24609

21625

23699

24G73-107times99HS-18

1398

1675

3370

2148

2753

8672

11918

7781

362

691

981

678

566746

00

3500

4589

170

247

270

229

24677

20567

17855

21033

2577245times

CVJ-2-5-3-1-3

1500

1728

2203

1810

2282

4679

11492

6151

328

424

1116

623

6000

4333

420048

44300

231

370

300

21257

20497

1772

519826

2677245times1305

143815772080

1698

949

10439

12053

7814

121

915

992

676

5767

4800

3500

4689

168246

316

244

1978

91779

015517

17699

2777245times(77371times

BSEC

TCP

-1)

150516972846

2016

3222

8518

12621

8120

469

827

1141

812

5400

4367

3833

4533

196

225

242

221

2092

819485

1719

219202

2877245times96AC

-93

1653

1797

2117

1856

1934

6056

11543

6511

352

563

911

608

5333

4267

3833

4478

186

169

198

184

22003

19054

1676

719275

2977245timesTo

go-II

1723

1718

3163

2201

1749

8250

15443

8481229

806

1412

816

5967

4700

4067

4911

174

245

298

239

22075

23503

19875

21818

3077245times99HS-18

2016

1970

28542280

3217

13027

3531

6591395

965

285

549

5733

4467

3833

4678

148

228

209

195

2212

721462

1690

020

163

31CV

J-2-5-3-1-3times

1305

1358

1422

2179

1653

1254

2803

1019

44750

178

254

801

411

6033

4467

3367

4622

312

193

330

278

22269

19547

17250

19689

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

1335

1742

2400

1826

922

5226

12806

6318

236

458

1054

583

5633

4433

3600

4556

321

315

259

298

2210

419524

17087

19572

33CV

J-2-5-3-1-3times

96AC

-93

1552

16803260

2164

166710094

11941

7901250

974

1115

779

5533

4533

446748

44

170

202

317

230

2392

123317

19333

2219

0

34CV

J-2-5-3-1-3times

Togo-II

142216072638

1889

1012

4163

11061

5412

261

377

891

510

6167

4967

4533

5222

207

225

325

252

23590

22860

20278

22243

35CV

J-2-5-3-1-3times

99HS-18

1435

1600

2700

1912

3056

3237

7089

4461449

301

658

469

5700

44673800

4656

148

178

225

183

22444

20240

1696

719884

361305times(77371times

BSEC

TCP

-1)

1499

1767

2058

1774

1882

4665

7920

4822

356

426

724

502

566742673800

4578

271

242

305

273

22011

19295

16417

19241

371305times96AC

-93

16781605

2757

2014

1516

10037

11960

7838

311

11381284

911

560040

673400

4356

182

276

251

236

2396

519625

15800

1979

738

1305timesTo

go-II

1177

16802249

1702

902

4676

9388

4989

241

456

795

497

6533

5533

4733

5600

214

266

296

259

21894

20217

19222

20444

391305times99HS-18

1774

1767

2766

2102

2145

4628

10618

5797

301

447

991

580

5933

4500

3733

4722

128

142

180

150

2275

719413

16967

1971

2

40(77371times

BSEC

TCP

-1)times

96AC

-93

1748

1795

31712238

1537

12586

14379

9501

211

1104

1183

833

546744

00346744

44

127

201

226

185

23294

21089

17858

2074

7

10 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(

days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

41(77371times

BSEC

TCP

-1)times

Togo-II

19652007

2653

2208

2136

5601

9503

5747

315

635

1001

650

5533

4833

410048

22

213

241

286

247

22662

22094

17667

20808

42(77371times

BSEC

TCP

-1)times

99HS-18

15011687

2705

1964

1935

7253

11806

6998

314

704

1067

695

5467

4433

5133

5011

177

167

175

173

22305

19277

16392

19325

4396AC

-93timesTo

go-II1759

1618

2123

1834

1539

10347

12212

8033

325

907

1090

774

5900

450039674789

214

269

339

274

23252

21554

16875

20560

4496AC

-93times

99HS-18

1353

1618

2925

1966

3532

7223

11950

7569

440

654

1024

706

5600

4900

4533

5011

149

175

206

177

24494

20698

18111

2110

145

Togo-IItimes

99HS-18

1117

1278

2582

1659

3448

6764

11910

7374

477

767

1207

817

6000

5133

4333

5156

183

203

216

201

17683

15541

14333

15853

Mean

1513

1618

2492

1874

1656

5773

9854

5761

272

538

885

565

586846

534116

4879

218

233

268

240

21297

19547

16529

1912

4SE

101

073

098

mdash079

152

317

mdash014

014

028

mdash351

365

428

mdash036

039

046

mdash985

1173

1211

mdashCD

284

205

276

mdash221

427

890

mdash038

040

079

mdash12

513

015

2mdash

013

014

016

mdash351

418

431

mdash

(c)

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

1642

1667

1744

1684

1504

1349

1526

1460

2783

2405

2634

2607

836

745

908

830

4287

3754

4160

4067

2H7729-2

1693

1817

1950

1820

1012

859

1016

962

3433

2809

2591

2944

649

597

651

632

4445

3668

3607

3906

3G73-107

2000

2028

2077

2035

2280

1966

2312

2186

4695

4023

4229

4316

1281

1170

1463

1305

6975

5989

6541

6502

477245

1523

1630

1728

1627

1796

2489

2702

2329

5423

4851

4498

4924

748

1464

1743

1319

7219

7341

7200

7253

5CV

J-2-5-3-1-3

2282

2692

2787

2587

2411

2643

2901

2652

5857

4836

5024

5239

1206

1335

1769

1437

8268

7479

7925

7891

61305

1851

1951

2054

1952

2026

1763

1908

1899

4657

4414

4463

4511

1468

1336

1539

1448

6683

6177

6371

6410

777371timesBS

ECTCP

-11629

1645

1733

1669

1244

1692

1884

1607

4925

4183

4435

4515

625

1092

1483

1067

6169

5875

6319

6121

896AC

-93

1379

1519

1600

1499

2066

2942

3088

2698

4501

4095

4229

4275

984

1565

1678

1409

6567

7037

7316

6973

9To

go-II

1904

2057

2103

2021

2167

1844

2358

2123

4520

3446

3904

3957

1298

1138

1502

1313

6687

5290

6262

6080

1099HS-18

1475

1485

1633

1531

3180

2904

3382

3155

5633

4139

5205

4992

1656

1536

1879

1690

8813

7043

8587

8147

1H77833-2timesH7729-2

2010

2046

2133

2063

3645

3075

4377

3699

7675

6540

7199

7138

2014

1708

2880

2201

11320

9615

11576

10837

2H77833-2timesG73-107

2066

2101

2122

2096

5002

4053

4601

4552

7882

7430

6963

7425

3146

2565

2808

2840

12884

11482

11564

11977

3H77833-2times77245

1764

2048

2083

1965

3769

4497

4559

4275

8681

8515

8475

8557

2284

3356

3377

3006

12450

13012

13034

12832

4H77833-2times

CVJ-2-5-3-1-3

2205

2383

2733

2441

3693

5655

5220

4856

11656

10287

1010

510683

2198

4488

4176

3621

15348

1594

215325

15538

5H77833-2times1305

2209

2423

2491

2374

6008

5842

6933

6261

15238

14311

13889

14479

3758

4057

5591

4469

21246

2015

320822

2074

0

6H77833-2times(77371times

BSEC

TCP

-1)

1789

1823

1992

1868

3593

4288

4903

4262

8922

6522

7429

7625

2178

3224

3070

2824

12515

10811

12333

11886

7H77833-2times96AC

-93

1739

1960

2011

1903

4557

4350

4818

4575

7916

6068

7290

7092

2411

2529

3191

2710

12474

10419

1210

811667

8H77833-2timesTo

go-II

1927

2389

2406

2241

4185

5828

6215

5409

12319

10279

11023

11207

2433

4254

4631

3773

16504

1610

717238

16616

9H77833-2times99HS-18

1941

2245

2330

2172

4227

4139

4529

4298

9211

8120

7969

8433

3154

3184

3253

3197

13438

12259

12498

1273

210

H7729-2timesG73-107

1968

2109

2158

2079

3725

3042

3488

3418

7313

5956

6174

6481

2822

2001

2357

2393

11038

8998

9662

9899

Advances in Agriculture 11

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

11H7729-2times77245

1847

2048

2200

2032

2467

3759

5466

3898

7672

7449

8921

8014

1356

2350

3796

2500

1013

911208

14387

11911

12H7729-2times

CVJ-2-5-3-1-3

2218

2585

2767

2523

3674

4372

4483

4176

10053

9467

8664

9395

2417

3312

3345

3025

1372

813839

1314

713571

13H7729-2times1305

2169

2338

2547

2352

6728

6166

6792

6562

13333

11662

13301

1276

54128

4501

5660

4763

20061

17828

20093

19327

14H7729-2times(77371times

BSEC

TCP

-1)

1925

2095

2127

2049

3530

4165

4649

4115

9638

6917

7698

8084

1681

2833

3150

2555

1316

8110

82

12347

1219

915

H7729-2times96AC

-93

2012

2070

2180

2087

4955

4447

4596

4666

8738

6882

7762

7794

3754

3558

3181

3498

13693

11329

12359

12460

16H7729-2timesTo

go-II

2138

2428

2563

2377

4949

4660

5118

4909

8549

7243

7191

7661

3586

3353

3028

3322

13498

11903

12310

12570

17H7729-2times99HS-18

1899

1933

2073

1969

4046

4527

4988

4520

8492

7895

8144

8177

2561

3378

3553

3164

12539

12422

1313

212698

18G73-107times77245

1840

2253

2437

2177

2580

4776

5915

4424

11673

10608

10459

10913

1358

3163

4286

2936

14253

15385

16374

15337

19G73-107times

CVJ-2-5-3-1-3

2324

2537

2691

2517

4958

5030

5599

5196

9524

9395

1114

610022

3241

3645

4444

3776

14483

14425

1674

515217

20G73-107times1305

2228

2433

2583

2415

6340

5794

6373

6169

12490

1015

8119

61

11536

4199

4199

5018

4472

18830

1595

218334

1770

6

21G73-107times(77371times

BSEC

TCP

-1)

1943

2128

2220

2097

3936

3980

5770

4562

11697

9858

10303

10619

1874

2369

4181

2808

15633

13839

16073

1518

222

G73-107times96AC

-93

2118

2218

2317

2217

4270

5843

6201

5438

10319

9872

1015

910117

2703

4460

4379

3847

14589

15715

16359

15555

23G73-107timesTo

go-II

2237

2365

2733

2445

5736

5175

6822

5911

12098

10808

1299

611967

3984

3670

5372

4342

17835

1598

219818

17878

24G73-107times99HS-18

2025

2081

2322

2143

3558

4229

6612

4800

12350

9685

11516

1118

42196

2782

5009

3329

1590

813914

1812

915983

2577245timesCV

J-2-5-3-1-3

2109

2360

2675

2381

6760

6558

7299

6872

15225

11861

1416

21374

94225

4752

5839

4939

2198

518418

21461

20621

2677245times1305

1919

2100

2197

2072

5700

5142

5347

5397

11687

10565

10737

10996

4318

3956

4244

4173

17386

1570

816085

16393

2777245times(77371times

BSEC

TCP

-1)

1851

1935

2020

1936

2577

2460

2838

2625

6989

4778

4636

5468

1631

1662

1999

1764

9566

7238

7474

8093

2877245times96AC

-93

1729

1780

2133

1881

5541

5058

5748

5449

11952

8174

9526

9884

3646

3587

4071

3768

17493

13232

15274

15333

2977245timesTo

go-II

2300

2508

2658

2489

3067

5431

5963

4820

11737

11021

1216

411641

1598

4053

4770

3474

14805

16452

1812

616461

3077245times99HS-18

1982

2019

2223

2075

3846

3119

4225

3730

7493

6720

6476

6896

3205

2578

2643

2809

11339

9839

1070

110626

12 Advances in Agriculture

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

31CV

J-2-5-3-1-3times1305

2406

2553

3067

2675

4178

4796

6281

5085

13822

11425

12252

12500

2472

3241

4907

3540

18000

16222

18532

17585

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

2142

2173

2238

2184

4522

5235

5640

5132

11673

9658

10248

10526

2614

3766

4178

3519

1619

514893

15888

15659

33CV

J-2-5-3-1-3times

96AC

-93

2350

2477

2600

2476

4676

4280

6090

5015

11975

9832

10868

10891

3097

2931

4445

3491

16651

14111

1695

815907

34CV

J-2-5-3-1-3times

Togo-II

2093

2666

2989

2583

4058

3727

5190

4325

12588

10717

11217

11507

2742

2570

4185

3166

16645

14443

16407

15832

35CV

J-2-5-3-1-3times

99HS-18

2028

2311

2386

2242

3287

2513

5356

3719

10055

9733

1036

410051

2004

1581

4251

2612

13342

12246

1572

01376

9

361305times(77371times

BSEC

TCP

-1)

2005

2173

2210

2129

3569

4692

5140

4467

11652

9826

10041

10507

2005

3665

4179

3283

15221

14518

1518

214974

371305times96AC

-93

1822

2003

2047

1957

5115

4023

4813

4650

9965

7675

7571

8404

3456

2647

3155

3086

15080

11698

12384

13054

381305timesTo

go-II

2545

2583

2644

2591

6885

7283

6795

6987

12265

11410

12583

12086

4500

6120

5350

5323

1915

018693

19378

19074

391305times99HS-18

2070

2408

2593

2357

5219

4332

4800

4784

1092

97677

8968

9192

3924

3332

3840

3699

1614

912009

1376

913976

40(77371times

BSEC

TCP

-1)

times96AC

-93

2056

2115

2157

2109

3158

4463

4717

4113

9166

7540

8100

8268

1880

3542

3347

2923

12324

12003

12817

12381

41(77371times

BSEC

TCP

-1)

timesTo

go-II

1868

2253

2450

2190

4422

6458

7038

5973

1415

312369

1379

713439

2291

4646

5586

4174

18575

18827

20835

19412

42(77371times

BSEC

TCP

-1)

times99HS-18

1995

2026

2209

2077

2720

2909

3543

3057

7392

4692

5023

5702

1766

2187

2147

2033

10112

7601

8566

8760

4396AC

-93timesTo

go-II

1953

2027

2511

2164

5972

4391

7126

5830

15537

1392

714432

14632

3780

2538

5655

3991

21510

18318

21558

20462

4496AC

-93times99HS-18

1952

2021

2242

2072

3465

2758

4496

3573

5959

5186

6094

5746

2707

2154

2409

2423

9424

7944

10590

9319

45To

go-IItimes

99HS-18

1932

2008

2658

2199

5381

4995

5436

5270

10299

8876

9244

9473

3986

3842

3668

3832

15680

13871

14680

1474

3Mean

1982

2146

2301

2143

3963

4123

4836

4307

9498

8087

8663

8749

2510

2914

3513

2979

13460

12210

13499

13056

SE243

198

245

mdash671

501

626

mdash959

750

822

mdash423

349

344

mdash1155

927

1167

mdashCD

087

071

087

mdash239

178

223

mdash342

267

293

mdash15

112

412

2mdash

411

330

416

mdash

Advances in Agriculture 13Ta

ble4Analysis

ofvaria

ncefor

10times10

half-diallelfor

different

charactersin

threee

nviro

nments

(a)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Germination

()

Rateof

emergence

STI()

SSTI

()

Num

bero

fleavesseedling

Seedlin

gheight

(cm)

Freshweight

(g)seedlin

gDry

weight

(g)seedlin

g

E 1

Replicates

2652731

596768

783393

20700

93018674

33764

1033999

002590

Treatm

ents

541097

0255lowastlowast

8181153lowastlowast

30863092lowastlowast

20680900lowastlowast

1100708lowastlowast

2106317lowastlowast

930829527lowastlowast

319767lowastlowast

Parents

95118

163lowastlowast

14289925lowastlowast

59114816lowastlowast

18880254lowastlowast

1084227lowastlowast

1087906lowastlowast

22561719lowastlowast

042162lowastlowast

Hybrid

s44

11423573lowastlowast

3398933lowastlowast

25697199lowastlowast

20633716lowastlowast

472874lowastlowast

1391526lowastlowast

674123518lowastlowast

261839lowastlowast

Parentsv

ersush

ybrid

s1

43693104lowastlowast

163619

916lowastlowast

3896838

3896

2793lowastlowast

28873741lowastlowast

4272

2798lowastlowast

234144289lowastlowast

5367022lowastlowast

Error

108

281184

1356832

1173684

674337

040104

306

462

2015

1619

005535

E 2

Replicates

2583819

3707389lowast

1596635

198738

034925

448213

899529

007177

Treatm

ents

549997443lowastlowast

2825177lowastlowast

35378177lowastlowast

19074306lowastlowast

665284lowastlowast

1764

081lowastlowast

257694570lowastlowast

2177519lowastlowast

Parents

96507784lowastlowast

3718

879lowastlowast

69866289lowastlowast

1798

5404lowastlowast

705379lowastlowast

1408518lowastlowast

10612519lowastlowast

077429lowastlowast

Hybrid

s44

10569731lowastlowast

2127302lowastlowast

2910

2537lowastlowast

19429240lowastlowast

329256lowastlowast

7000

03lowastlowast

186026552lowastlowast

1483428lowastlowast

Parentsv

ersush

ybrid

s1

16223710lowastlowast

25488361lowastlowast

1113

326

13257362lowastlowast

15089673lowastlowast

5178

3569lowastlowast

5634825791lowastlowast

51618348lowastlowast

Error

108

334760

1097039

1049505

635570

06764

116

0576

694613

006206

E 3

Replicates

2894398

4808815lowast

mdashmdash

051076

095989

3768421

030220

Treatm

ents

541261560

0lowastlowast

4842216lowastlowast

mdashmdash

2811077lowastlowast

5947316lowastlowast

3870844

50lowastlowast

3445076lowastlowast

Parents

97135

735lowastlowast

1097

6388lowastlowast

mdashmdash

1468699lowastlowast

1112

764lowastlowast

136081650lowastlowast

1307836lowastlowast

Hybrid

s44

13217740lowastlowast

1379360

mdashmdash

15540

71lowastlowast

4581632lowastlowast

252084767lowastlowast

2378032lowastlowast

Parentsv

ersush

ybrid

s1

35440

180lowastlowast

102000335lowastlowast

mdashmdash

70200767lowastlowast

109548362lowastlowast

8586095705lowastlowast

69630188lowastlowast

Error

108

782907

1085444

mdashmdash

139771

291325

3019

169

02400

4(b)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Panicle

emergence

Effectiv

etillersplant

Planth

eight

Earlength

Earw

eightp

lant

Dry

fodd

eryieldplant

Grain

yieldplant

Totalbiological

yieldplant

E 1

Replicates

231860

61lowastlowast

416348lowastlowast

1672

9472lowast

1706

411077192

9634539

392777

115544

59Treatm

ents

544152

368lowastlowast

085657lowastlowast

326706089lowastlowast

1602572lowastlowast

6177

0406lowastlowast

304113

204lowastlowast

34285030lowastlowast

575617

582lowastlowast

Parents

94511481lowastlowast

018538lowastlowast

140807476lowastlowast

2240093lowastlowast

11653

959lowastlowast

27014155lowastlowast

3858650lowastlowast

61321919lowastlowast

Hybrid

s44

3201347lowastlowast

100797lowastlowast

110395657lowastlowast

1010

497lowastlowast

40296671lowastlowast

171271411lowastlowast

2413

6880lowastlowast

302999650lowastlowast

Parentsv

ersush

ybrid

s1

4276

5286lowastlowast

02360

9lowast11517452

616lowastlowast

2191

6167lowastlowast

1457662749lowastlowast

8643043524lowastlowast

7546

41053lowastlowast

17199467546lowastlowast

Error

108

468159

004907

3692099

224610

17114

303498126

682350

5075268

E 2

Replicates

2656364

002556

61575565lowastlowast

828842lowastlowast

589094

2268506

260877

1507116

Treatm

ents

542999618lowastlowast

069822lowastlowast

268998723lowastlowast

2436886lowastlowast

60592784lowastlowast

247259877lowastlowast

4192

0073lowastlowast

513483489lowastlowast

Parents

926300

00lowastlowast

048079lowastlowast

165555

405lowastlowast

3871874lowastlowast

1412

2393lowastlowast

19543682lowastlowast

3132

426lowastlowast

57230706lowastlowast

Hybrid

s44

2533

165lowastlowast

07344

9lowastlowast

9616

2857lowastlowast

1463359lowastlowast

35495366lowastlowast

154759288lowastlowast

26262465lowastlowast

293492304lowastlowast

Parentsv

ersush

ybrid

s1

26850135lowastlowast

105929lowastlowast

880476

6697lowastlowast

32357161lowastlowast

1583112746lowastlowast

6366731560lowastlowast

107994

3646lowastlowast

14299370699lowastlowast

Error

108

507598

005829

5234122

149406

955133

2142411

462311

3273886

E 3

Replicates

2029697

021161

9459847

143746

985141

46800

6492

6334

3345160

Treatm

ents

548045499lowastlowast

094511lowastlowast

192420108lowastlowast

3279225lowastlowast

72049211lowastlowast

298953

021lowastlowast

55061308lowastlowast

651732994lowastlowast

Parents

98010

741lowastlowast

094166lowastlowast

116037343lowastlowast

3702185lowastlowast

16305190lowastlowast

2323644

0lowastlowast

4553

893lowastlowast

71484982lowastlowast

Hybrid

s44

6254377lowastlowast

095514lowastlowast

7378

1513lowastlowast

2188348lowastlowast

31813193lowastlowast

190263916lowastlowast

31572179lowastlowast

368689934lowastlowast

Parentsv

ersush

ybrid

s1

8716

7710lowastlowast

053496lowast

609996

3199lowastlowast

47471212lowastlowast

2344

130203lowastlowast

756272

2861lowastlowast

154314

9707lowastlowast

18327859

719lowastlowast

Error

108

696981

007883

5578505

228608

1491014

2574

223

449588

5187705

lowast

Sign

ificant

at119875=005

lowastlowastSign

ificant

at119875=001

14 Advances in Agriculture

Table 5 Mean performance of the genotypes for membrane thermostability in two environments

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594

Advances in Agriculture 15

Table 5 Continued

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434

Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash

lowastValues are transformed values

0

6

12

18

24

30

36

42

48

54

minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity

G73-10796AC-93CVJ-2-5-3-1-3

Togo-II99HS-18

H77833-2H7729-2772451305

H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305

1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305

H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305

77245 times 1305

77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305

77371 times BSECT CP-1

H77833-2 times G73-107

Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits

moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling

survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The

16 Advances in Agriculture

Table6Po

oled

correla

tioncoeffi

cientamon

gvario

uscharactersin

threee

nviro

nments

Characters

Germination

Rateof

emergence

STI

SSTI

Leaves

seedlin

gSeedlin

gheight

Fresh

weight

Dry

weight

MTS

Panicle

emergence

Effectiv

etillersplant

Plant

height

Earlength

Ear

weightp

lant

Dry

fodd

eryield

plant

Grain

yield

plant

Total

biological

yield

plant

Germination

1000

0357lowastlowast

0353lowastlowast

0590lowastlowast

0230

0373lowastlowast

0293lowast

0260

0276lowastminus0135minus0478lowastlowast

0326lowast

minus0069minus0104

minus0190

minus0145

minus0167

Rateof

emergence

1000

0233

0351lowastlowast

0605lowastlowast

0585lowastlowast

0551lowastlowast

0524lowastlowast

0139minus0593lowastlowast

0032

0517lowastlowast

0217

0388lowastlowast

0361lowastlowast

0348lowastlowast

0377lowastlowast

STI

1000

0837lowastlowast

0241

0129

0081

004

80890lowastlowast

minus0215

minus0211

0030minus0007minus0043

minus0101

minus0111

minus0084

SSTI

1000

0233

0264

0183

0140

0745lowastlowast

minus0247minus0268lowast

0184minus0017minus006

6minus0129

minus0144

minus0112

Num

bero

fleaves

1000

0683lowastlowast

0633lowastlowast

060

4lowastlowast

0176minus0474lowastlowast

0109

0576lowastlowast

0496lowastlowast

064

1lowastlowast

0607lowastlowast

0630lowastlowast

0630lowastlowast

Seedlin

gheight

1000

0703lowastlowast

0663lowastlowast

0031minus0493lowastlowast

minus0115

0735lowastlowast

0360lowastlowast

0526lowastlowast

0520lowastlowast

0515lowastlowast

0532lowastlowast

Freshweight

1000

0957lowastlowast

minus0016

0425lowastlowast

minus0082

0661lowastlowast

0351lowastlowast

0581lowastlowast

0496lowastlowast

0576lowastlowast

0533lowastlowast

Dry

weight

1000minus0033minus0428lowastlowast

minus0016

0634lowastlowast

0354lowastlowast

0620lowastlowast

0509lowastlowast

0614lowastlowast

0554lowastlowast

MTS

1000minus040

minus0177minus0037

0015

minus0074

minus0138

minus0131

minus0120

Panicle

emergence

1000

minus0150minus0269lowast

0022minus0329lowast

minus0337lowast

minus0280lowast

minus0341lowast

Effectiv

etillersplant

1000

minus0012

0170

0366lowastlowast

040

5lowastlowast

0323lowast

0401lowastlowast

Planth

eight

1000

0650lowastlowast

0603lowastlowast

0596lowastlowast

0619lowastlowast

0610lowastlowast

Earlength

1000

0608lowastlowast

0671lowastlowast

064

8lowastlowast

066

4lowastlowast

Earw

eightp

lant

1000

0910lowastlowast

0984lowastlowast

0957lowastlowast

Dry

fodd

eryield

plant

1000

0907lowastlowast

0991lowastlowast

Grain

yield

plant

1000

0950lowastlowast

Totalbiological

yield

plant

1000

lowastSign

ificant

at119875=005lowastlowastSign

ificant

at119875=001

Advances in Agriculture 17

faster germination and early vigour in seedling could act asphysical indicators for heat tolerance

Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits

Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics

Conflict of Interests

The authors declare that there is no conflict of interestsregarding the publication of this paper

References

[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981

[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993

[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006

[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993

[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013

[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007

[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965

[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011

[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001

[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009

[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962

[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967

[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963

[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001

Submit your manuscripts athttpwwwhindawicom

Nutrition and Metabolism

Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

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Agronomy

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Microbiology

The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom

Applied ampEnvironmentalSoil Science

Volume 2014

AgricultureAdvances in

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

BiodiversityInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

GenomicsInternational Journal of

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Plant GenomicsInternational Journal of

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Biotechnology Research International

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Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Page 7: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

Advances in Agriculture 7

(a)Con

tinued

Sr

No

Genotypes

Germination(

)Ra

teof

emergencelowast

STIlowast

()

SSTIlowast(

)Num

bero

fleavesseedlin

gE 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

2877245times96AC

-93

5472

4705

622154

66

5135

5197

5006

5113

5247

4843

9000

6363

4708

3784

9000

5831

795

662

1058

838

2977245timesTo

go-II

5252

4492

5889

5211

5188

4861

5074

5041

4885

4263

9000

6049

4421

3379

9000

5600

977

751

1243

990

3077245times99HS-18

5740

4099

6872

5570

5249

4898

5258

5135

5628

4616

9000

6415

4863

3014

9000

5626

1045

863

1184

1031

31CV

J-2-5-3-1-3times1305

4364

3944

5700

4669

4974

4517

4650

4714

6097

5579

9000

6892

4594

3842

9000

5812

936

848

1403

1063

32CV

J-2-5-3-1-3times(77371times

BSEC

TCP

-1)4345

3808

6774

4976

5272

4996

5035

5101

6650

5766

9000

7139

4288

3381

9000

5556

800

712

1013

841

33CV

J-2-5-3-1-3times96AC

-93

4862

4197

5743

4934

4884

4980

5058

4974

7477

6760

9000

7745

5957

4709

9000

6555

933

738

1478

1050

34CV

J-2-5-3-1-3timesTo

go-II

4670

4343

6233

5082

4399

4813

4631

4614

5806

6614

9000

7140

4422

4479

9000

5967

963

855

1180

999

35CV

J-2-5-3-1-3times99HS-18

5493

5080

7273

5949

5276

4984

5162

5141

7322

6590

9000

7637

5534

4766

9000

6433

998

807

1338

1047

361305times(77371times

BSEC

TCP

-1)

4613

4539

5717

4956

4986

4955

5080

5007

5137

4870

9000

6336

4210

3936

9000

5715

863

630

913

802

371305times96AC

-93

5473

5177

6830

5827

5224

4779

4919

4974

7117

6965

9000

7694

5640

5220

9000

6620

875

694

1476

1015

381305timesTo

go-II

4230

3420

5262

4304

4309

456144

45

4438

5781

4373

9000

6385

4575

2887

9000

5487

637

566

794

666

391305times99HS-18

5433

4507

6947

5629

5225

4753

4991

4989

7287

6968

9000

7752

5576

4479

9000

6352

1115

818

1410

1114

40(77371times

BSEC

TCP

-1)times

96AC

-93

5595

4429

7461

5828

5216

4955

5121

5097

7087

6368

9000

7485

5432

4038

9000

6157

955

753

1462

1057

41(77371times

BSEC

TCP

-1)times

Togo-II

5252

4728

6106

5362

5492

4808

5132

5144

8025

7505

9000

8177

6310

5413

9000

6908

859

693

990

848

42(77371times

BSEC

TCP

-1)times

99HS-18

5595

4298

6295

5396

4729

4887

5031

4883

7366

6945

9000

7770

6312

4556

9000

6623

903

622

1125

883

4396AC

-93timesTo

go-II

5017

3977

6486

5160

5019

5195

5237

5150

5922

5486

9000

6803

4689

3484

9000

5724

1007

728

1167

967

4496AC

-93times99HS-18

5595

4812

6924

5777

5036

5385

5205

5209

7411

5378

9000

7263

5873

3979

9000

6284

1013

652

1334

1000

45To

go-IItimes

99HS-18

4670

4497

7293

5487

4718

4319

4796

4611

5688

5431

9000

6706

3980

3665

9000

5548

939

653

1462

1018

Mean

5043

4482

6271

5265

4792

498149

23

4899

6377

584190

00

7072

5079

4190

9000

6090

898

701

1168

922

SE097

106

161

mdash213

191

190

mdash19

818

7mdash

mdash14

914

6mdash

mdash036

047

068

mdashCD

272

296

453

mdash597

537

534

mdash555

525

mdashmdash

421

409

mdashmdash

103

133

192

mdashlowastTransfo

rmed

data

(b)

Sr

No

Genotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

908

785

2245

1313

859

1537

1924

1440

154

143

160

152

6267

4800

3833

4967

212

227

249

229

15644

15458

13267

1479

02

H7729-2

1125

1175

1789

1363

317

2400

2811

1843

093

191

252

179

6700

5333

5100

5711

185

210

234

210

18850

18565

15389

17601

3G73-107

1166

1475

1966

1536

943

1744

4945

2544

143

129

366

213

6700

5433

5300

5811

218

219

238

225

15009

1496

513503

14492

477245

1438

1373

2085

1632

607

1863

5957

2809

117

180

509

269

6100

4800

5100

5333

232

221

253

235

14036

13802

11742

1319

35

CVJ-2-5-3-1-3

1214

1437

1807

1486

1163

1610

4166

2313

171

141

407

240

6300

4933

4600

5278

239

227

270

245

1994

317261

1497

017391

61305

858

1137

1737

1244

540

967

4861

2123

125

088

417

210

6533

5033

4800

5456

244

213

257

238

15527

15405

12274

14402

8 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

777371timesBS

ECT

CP-1

1377

1487

1892

1585

928

2660

3330

2306

150

258

301

236

5733

4533

4700

4989

242

220

293

252

1513

814550

1315

014279

896AC

-93

1303

1259

1852

1471

1056

1445

5972

2825

185

147

586

306

5500

4833

3833

4722

256

304

318

293

15577

14032

10000

13203

9To

go-II

1056

1220

2272

1516

924

1466

9113

3834

146

129

883

386

6167

4533

4600

5100

185

149

124

153

14578

12073

10033

12228

1099HS-18

1277

1075

1808

1387

1241

2833

7070

3715

228

226

615

356

6100

5000

4167

5089

243

173

324

247

12627

10355

1017

311052

1H77833-2times

H7729-2

1432

1498

2233

1721

1350

4405

8373

4709

159

499

782

480

5767

4433

3800

4667309

290

248

282

21010

18071

15847

18309

2H77833-2times

G73-107

1675

1635

2205

1838

1236

3211

5789

3412

134

313

547

331

5833

4767

3833

4811

262

295

236

265

21253

20449

15808

1917

0

3H77833-2times

77245

1577

1522

2263

1787

1058

6933

8719

5570

255

553

782

530

5267

4633

4600

4833

273

201

265

246

2074

119511

15208

18487

4H77833-2times

CVJ-2-5-3-1-3

1791

1757

2165

1904

20218030

5276

5109

354

779

477

537

5533

4233

3767

4511

165

330

351

282

21827

20072

17110

19670

5H77833-2times1305

1700

1842

28412128

1427

6228

9134

5596

570

579

813

654

5800

4267

3333

4467340

426

451

406

19816

19444

16037

18432

6H77833-2times

(77371times

BSEC

TCP

-1)

1453

1515

3120

2029

789

465510428

5291

211

408

858

492

5233

4167

3267

4222

203

283

267

251

20677

1913

816470

1876

2

7H77833-2times

96AC

-93

1835

1783

2427

2015

2532

6649

1610

684

29

350

650

1473

824

5467

4233

3700

4467243

239

256

246

22278

20434

15735

19482

8H77833-2times

Togo-II

1564

15632500

1876

1882

4772

11660

6105

296

469

972

579

5667

4533

440048

67

169

260

211

213

2412

82375

619389

22424

9H77833-2times

99HS-18

159318002049

1814

1571

480214024

6799

203

544

1547

765

6033

460040

0048

78

261

225

256

247

23466

21081

1793

320

827

10H7729-2times

G73-107

14951568

2075

1713

959

5157

8517

4877

218

382

666

422

6133

5067

4833

5344

300

273

239

271

23651

2191

717083

20884

11H7729-2times77245

1557

1537

2613

1902

1851

5756

5729

4445366

562

524

484

5633

4800

4633

5022

133

233

297

221

1979

219914

15250

18319

12H7729-2times

CVJ-2-5-3-1-3

18442033

28932257

1651

806114885

8199

264

597

1054

638

6333

4600

4233

5056

170

211

217

199

24629

23339

1970

022556

13H7729-2times1305

1325

1677309920

34

1756

724314048

7682

318

635

1232

728

630046

67

3633

4867298

261

361

307

21666

19371

17422

19486

14H7729-2times(77371

timesBS

ECTCP

-1)

1958

1887

3105

2317

1118

4675

14411

6734

242

457

1318

672

5900

4500

3800

4733

164

209

240

204

23661

1995

419250

20955

15H7729-2times

96AC

-93

1512

1717

2779

2002266844

08

11393

6156

356

500

1211

689

5633

4533

4200

4789

221

195

282

232

2194

921635

17213

20266

Advances in Agriculture 9

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

16H7729-2times

Togo-II

1519

1738

2159

1805

2437

9867

1616

294

89

361

961

1527

950

6167

4700

4200

5022

234

272

230

246

26338

2414

520553

23678

17H7729-2times

99HS-18

1938

1787

3151

2292

1574

6218

11318

6370

290

606

956

617

6067

4767

406749

67

172

210

199

194

24689

21854

1778

921444

18G73-107times77245

1856

1813

2210

1960

2435

8550

7828

6271338

633

612

528

5500

4633

440048

44

199

255

307

254

1993

619277

16095

18436

19G73-107times

CVJ-2-5-3-1-3

1517

1769

2407

1897

1556

7728

9136

6140

239

876

917

677

5900

4300

3400

4533

233

266

315

272

23418

22333

19317

21689

20G73-107times1305

1217

1507

2832

1852

1777

5189

1114

960

38

289

588

1197

691

6733

5000

4233

5322

286

210

312

269

2078

81992

817067

19261

21G73-107times(77371

timesBS

ECTCP

-1)

18551946

2730

2177

1801

3482

10415

5233

239

277

753

423

5500

4467

3433

4467243

197

273

238

23817

20263

17425

20502

22G73-107times

96AC

-93

1718

1840

28612140

1345

9333

14669

8449

297

845

1278

807

5600

5033

4633

5089

270

243

300

271

28537

21461

16875

22291

23G73-107timesTo

go-II

168018083087

2192

1112

4678

9319

5036

186

473

985

548

6033

4767

4200

5000

210

209

231

217

24864

24609

21625

23699

24G73-107times99HS-18

1398

1675

3370

2148

2753

8672

11918

7781

362

691

981

678

566746

00

3500

4589

170

247

270

229

24677

20567

17855

21033

2577245times

CVJ-2-5-3-1-3

1500

1728

2203

1810

2282

4679

11492

6151

328

424

1116

623

6000

4333

420048

44300

231

370

300

21257

20497

1772

519826

2677245times1305

143815772080

1698

949

10439

12053

7814

121

915

992

676

5767

4800

3500

4689

168246

316

244

1978

91779

015517

17699

2777245times(77371times

BSEC

TCP

-1)

150516972846

2016

3222

8518

12621

8120

469

827

1141

812

5400

4367

3833

4533

196

225

242

221

2092

819485

1719

219202

2877245times96AC

-93

1653

1797

2117

1856

1934

6056

11543

6511

352

563

911

608

5333

4267

3833

4478

186

169

198

184

22003

19054

1676

719275

2977245timesTo

go-II

1723

1718

3163

2201

1749

8250

15443

8481229

806

1412

816

5967

4700

4067

4911

174

245

298

239

22075

23503

19875

21818

3077245times99HS-18

2016

1970

28542280

3217

13027

3531

6591395

965

285

549

5733

4467

3833

4678

148

228

209

195

2212

721462

1690

020

163

31CV

J-2-5-3-1-3times

1305

1358

1422

2179

1653

1254

2803

1019

44750

178

254

801

411

6033

4467

3367

4622

312

193

330

278

22269

19547

17250

19689

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

1335

1742

2400

1826

922

5226

12806

6318

236

458

1054

583

5633

4433

3600

4556

321

315

259

298

2210

419524

17087

19572

33CV

J-2-5-3-1-3times

96AC

-93

1552

16803260

2164

166710094

11941

7901250

974

1115

779

5533

4533

446748

44

170

202

317

230

2392

123317

19333

2219

0

34CV

J-2-5-3-1-3times

Togo-II

142216072638

1889

1012

4163

11061

5412

261

377

891

510

6167

4967

4533

5222

207

225

325

252

23590

22860

20278

22243

35CV

J-2-5-3-1-3times

99HS-18

1435

1600

2700

1912

3056

3237

7089

4461449

301

658

469

5700

44673800

4656

148

178

225

183

22444

20240

1696

719884

361305times(77371times

BSEC

TCP

-1)

1499

1767

2058

1774

1882

4665

7920

4822

356

426

724

502

566742673800

4578

271

242

305

273

22011

19295

16417

19241

371305times96AC

-93

16781605

2757

2014

1516

10037

11960

7838

311

11381284

911

560040

673400

4356

182

276

251

236

2396

519625

15800

1979

738

1305timesTo

go-II

1177

16802249

1702

902

4676

9388

4989

241

456

795

497

6533

5533

4733

5600

214

266

296

259

21894

20217

19222

20444

391305times99HS-18

1774

1767

2766

2102

2145

4628

10618

5797

301

447

991

580

5933

4500

3733

4722

128

142

180

150

2275

719413

16967

1971

2

40(77371times

BSEC

TCP

-1)times

96AC

-93

1748

1795

31712238

1537

12586

14379

9501

211

1104

1183

833

546744

00346744

44

127

201

226

185

23294

21089

17858

2074

7

10 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(

days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

41(77371times

BSEC

TCP

-1)times

Togo-II

19652007

2653

2208

2136

5601

9503

5747

315

635

1001

650

5533

4833

410048

22

213

241

286

247

22662

22094

17667

20808

42(77371times

BSEC

TCP

-1)times

99HS-18

15011687

2705

1964

1935

7253

11806

6998

314

704

1067

695

5467

4433

5133

5011

177

167

175

173

22305

19277

16392

19325

4396AC

-93timesTo

go-II1759

1618

2123

1834

1539

10347

12212

8033

325

907

1090

774

5900

450039674789

214

269

339

274

23252

21554

16875

20560

4496AC

-93times

99HS-18

1353

1618

2925

1966

3532

7223

11950

7569

440

654

1024

706

5600

4900

4533

5011

149

175

206

177

24494

20698

18111

2110

145

Togo-IItimes

99HS-18

1117

1278

2582

1659

3448

6764

11910

7374

477

767

1207

817

6000

5133

4333

5156

183

203

216

201

17683

15541

14333

15853

Mean

1513

1618

2492

1874

1656

5773

9854

5761

272

538

885

565

586846

534116

4879

218

233

268

240

21297

19547

16529

1912

4SE

101

073

098

mdash079

152

317

mdash014

014

028

mdash351

365

428

mdash036

039

046

mdash985

1173

1211

mdashCD

284

205

276

mdash221

427

890

mdash038

040

079

mdash12

513

015

2mdash

013

014

016

mdash351

418

431

mdash

(c)

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

1642

1667

1744

1684

1504

1349

1526

1460

2783

2405

2634

2607

836

745

908

830

4287

3754

4160

4067

2H7729-2

1693

1817

1950

1820

1012

859

1016

962

3433

2809

2591

2944

649

597

651

632

4445

3668

3607

3906

3G73-107

2000

2028

2077

2035

2280

1966

2312

2186

4695

4023

4229

4316

1281

1170

1463

1305

6975

5989

6541

6502

477245

1523

1630

1728

1627

1796

2489

2702

2329

5423

4851

4498

4924

748

1464

1743

1319

7219

7341

7200

7253

5CV

J-2-5-3-1-3

2282

2692

2787

2587

2411

2643

2901

2652

5857

4836

5024

5239

1206

1335

1769

1437

8268

7479

7925

7891

61305

1851

1951

2054

1952

2026

1763

1908

1899

4657

4414

4463

4511

1468

1336

1539

1448

6683

6177

6371

6410

777371timesBS

ECTCP

-11629

1645

1733

1669

1244

1692

1884

1607

4925

4183

4435

4515

625

1092

1483

1067

6169

5875

6319

6121

896AC

-93

1379

1519

1600

1499

2066

2942

3088

2698

4501

4095

4229

4275

984

1565

1678

1409

6567

7037

7316

6973

9To

go-II

1904

2057

2103

2021

2167

1844

2358

2123

4520

3446

3904

3957

1298

1138

1502

1313

6687

5290

6262

6080

1099HS-18

1475

1485

1633

1531

3180

2904

3382

3155

5633

4139

5205

4992

1656

1536

1879

1690

8813

7043

8587

8147

1H77833-2timesH7729-2

2010

2046

2133

2063

3645

3075

4377

3699

7675

6540

7199

7138

2014

1708

2880

2201

11320

9615

11576

10837

2H77833-2timesG73-107

2066

2101

2122

2096

5002

4053

4601

4552

7882

7430

6963

7425

3146

2565

2808

2840

12884

11482

11564

11977

3H77833-2times77245

1764

2048

2083

1965

3769

4497

4559

4275

8681

8515

8475

8557

2284

3356

3377

3006

12450

13012

13034

12832

4H77833-2times

CVJ-2-5-3-1-3

2205

2383

2733

2441

3693

5655

5220

4856

11656

10287

1010

510683

2198

4488

4176

3621

15348

1594

215325

15538

5H77833-2times1305

2209

2423

2491

2374

6008

5842

6933

6261

15238

14311

13889

14479

3758

4057

5591

4469

21246

2015

320822

2074

0

6H77833-2times(77371times

BSEC

TCP

-1)

1789

1823

1992

1868

3593

4288

4903

4262

8922

6522

7429

7625

2178

3224

3070

2824

12515

10811

12333

11886

7H77833-2times96AC

-93

1739

1960

2011

1903

4557

4350

4818

4575

7916

6068

7290

7092

2411

2529

3191

2710

12474

10419

1210

811667

8H77833-2timesTo

go-II

1927

2389

2406

2241

4185

5828

6215

5409

12319

10279

11023

11207

2433

4254

4631

3773

16504

1610

717238

16616

9H77833-2times99HS-18

1941

2245

2330

2172

4227

4139

4529

4298

9211

8120

7969

8433

3154

3184

3253

3197

13438

12259

12498

1273

210

H7729-2timesG73-107

1968

2109

2158

2079

3725

3042

3488

3418

7313

5956

6174

6481

2822

2001

2357

2393

11038

8998

9662

9899

Advances in Agriculture 11

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

11H7729-2times77245

1847

2048

2200

2032

2467

3759

5466

3898

7672

7449

8921

8014

1356

2350

3796

2500

1013

911208

14387

11911

12H7729-2times

CVJ-2-5-3-1-3

2218

2585

2767

2523

3674

4372

4483

4176

10053

9467

8664

9395

2417

3312

3345

3025

1372

813839

1314

713571

13H7729-2times1305

2169

2338

2547

2352

6728

6166

6792

6562

13333

11662

13301

1276

54128

4501

5660

4763

20061

17828

20093

19327

14H7729-2times(77371times

BSEC

TCP

-1)

1925

2095

2127

2049

3530

4165

4649

4115

9638

6917

7698

8084

1681

2833

3150

2555

1316

8110

82

12347

1219

915

H7729-2times96AC

-93

2012

2070

2180

2087

4955

4447

4596

4666

8738

6882

7762

7794

3754

3558

3181

3498

13693

11329

12359

12460

16H7729-2timesTo

go-II

2138

2428

2563

2377

4949

4660

5118

4909

8549

7243

7191

7661

3586

3353

3028

3322

13498

11903

12310

12570

17H7729-2times99HS-18

1899

1933

2073

1969

4046

4527

4988

4520

8492

7895

8144

8177

2561

3378

3553

3164

12539

12422

1313

212698

18G73-107times77245

1840

2253

2437

2177

2580

4776

5915

4424

11673

10608

10459

10913

1358

3163

4286

2936

14253

15385

16374

15337

19G73-107times

CVJ-2-5-3-1-3

2324

2537

2691

2517

4958

5030

5599

5196

9524

9395

1114

610022

3241

3645

4444

3776

14483

14425

1674

515217

20G73-107times1305

2228

2433

2583

2415

6340

5794

6373

6169

12490

1015

8119

61

11536

4199

4199

5018

4472

18830

1595

218334

1770

6

21G73-107times(77371times

BSEC

TCP

-1)

1943

2128

2220

2097

3936

3980

5770

4562

11697

9858

10303

10619

1874

2369

4181

2808

15633

13839

16073

1518

222

G73-107times96AC

-93

2118

2218

2317

2217

4270

5843

6201

5438

10319

9872

1015

910117

2703

4460

4379

3847

14589

15715

16359

15555

23G73-107timesTo

go-II

2237

2365

2733

2445

5736

5175

6822

5911

12098

10808

1299

611967

3984

3670

5372

4342

17835

1598

219818

17878

24G73-107times99HS-18

2025

2081

2322

2143

3558

4229

6612

4800

12350

9685

11516

1118

42196

2782

5009

3329

1590

813914

1812

915983

2577245timesCV

J-2-5-3-1-3

2109

2360

2675

2381

6760

6558

7299

6872

15225

11861

1416

21374

94225

4752

5839

4939

2198

518418

21461

20621

2677245times1305

1919

2100

2197

2072

5700

5142

5347

5397

11687

10565

10737

10996

4318

3956

4244

4173

17386

1570

816085

16393

2777245times(77371times

BSEC

TCP

-1)

1851

1935

2020

1936

2577

2460

2838

2625

6989

4778

4636

5468

1631

1662

1999

1764

9566

7238

7474

8093

2877245times96AC

-93

1729

1780

2133

1881

5541

5058

5748

5449

11952

8174

9526

9884

3646

3587

4071

3768

17493

13232

15274

15333

2977245timesTo

go-II

2300

2508

2658

2489

3067

5431

5963

4820

11737

11021

1216

411641

1598

4053

4770

3474

14805

16452

1812

616461

3077245times99HS-18

1982

2019

2223

2075

3846

3119

4225

3730

7493

6720

6476

6896

3205

2578

2643

2809

11339

9839

1070

110626

12 Advances in Agriculture

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

31CV

J-2-5-3-1-3times1305

2406

2553

3067

2675

4178

4796

6281

5085

13822

11425

12252

12500

2472

3241

4907

3540

18000

16222

18532

17585

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

2142

2173

2238

2184

4522

5235

5640

5132

11673

9658

10248

10526

2614

3766

4178

3519

1619

514893

15888

15659

33CV

J-2-5-3-1-3times

96AC

-93

2350

2477

2600

2476

4676

4280

6090

5015

11975

9832

10868

10891

3097

2931

4445

3491

16651

14111

1695

815907

34CV

J-2-5-3-1-3times

Togo-II

2093

2666

2989

2583

4058

3727

5190

4325

12588

10717

11217

11507

2742

2570

4185

3166

16645

14443

16407

15832

35CV

J-2-5-3-1-3times

99HS-18

2028

2311

2386

2242

3287

2513

5356

3719

10055

9733

1036

410051

2004

1581

4251

2612

13342

12246

1572

01376

9

361305times(77371times

BSEC

TCP

-1)

2005

2173

2210

2129

3569

4692

5140

4467

11652

9826

10041

10507

2005

3665

4179

3283

15221

14518

1518

214974

371305times96AC

-93

1822

2003

2047

1957

5115

4023

4813

4650

9965

7675

7571

8404

3456

2647

3155

3086

15080

11698

12384

13054

381305timesTo

go-II

2545

2583

2644

2591

6885

7283

6795

6987

12265

11410

12583

12086

4500

6120

5350

5323

1915

018693

19378

19074

391305times99HS-18

2070

2408

2593

2357

5219

4332

4800

4784

1092

97677

8968

9192

3924

3332

3840

3699

1614

912009

1376

913976

40(77371times

BSEC

TCP

-1)

times96AC

-93

2056

2115

2157

2109

3158

4463

4717

4113

9166

7540

8100

8268

1880

3542

3347

2923

12324

12003

12817

12381

41(77371times

BSEC

TCP

-1)

timesTo

go-II

1868

2253

2450

2190

4422

6458

7038

5973

1415

312369

1379

713439

2291

4646

5586

4174

18575

18827

20835

19412

42(77371times

BSEC

TCP

-1)

times99HS-18

1995

2026

2209

2077

2720

2909

3543

3057

7392

4692

5023

5702

1766

2187

2147

2033

10112

7601

8566

8760

4396AC

-93timesTo

go-II

1953

2027

2511

2164

5972

4391

7126

5830

15537

1392

714432

14632

3780

2538

5655

3991

21510

18318

21558

20462

4496AC

-93times99HS-18

1952

2021

2242

2072

3465

2758

4496

3573

5959

5186

6094

5746

2707

2154

2409

2423

9424

7944

10590

9319

45To

go-IItimes

99HS-18

1932

2008

2658

2199

5381

4995

5436

5270

10299

8876

9244

9473

3986

3842

3668

3832

15680

13871

14680

1474

3Mean

1982

2146

2301

2143

3963

4123

4836

4307

9498

8087

8663

8749

2510

2914

3513

2979

13460

12210

13499

13056

SE243

198

245

mdash671

501

626

mdash959

750

822

mdash423

349

344

mdash1155

927

1167

mdashCD

087

071

087

mdash239

178

223

mdash342

267

293

mdash15

112

412

2mdash

411

330

416

mdash

Advances in Agriculture 13Ta

ble4Analysis

ofvaria

ncefor

10times10

half-diallelfor

different

charactersin

threee

nviro

nments

(a)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Germination

()

Rateof

emergence

STI()

SSTI

()

Num

bero

fleavesseedling

Seedlin

gheight

(cm)

Freshweight

(g)seedlin

gDry

weight

(g)seedlin

g

E 1

Replicates

2652731

596768

783393

20700

93018674

33764

1033999

002590

Treatm

ents

541097

0255lowastlowast

8181153lowastlowast

30863092lowastlowast

20680900lowastlowast

1100708lowastlowast

2106317lowastlowast

930829527lowastlowast

319767lowastlowast

Parents

95118

163lowastlowast

14289925lowastlowast

59114816lowastlowast

18880254lowastlowast

1084227lowastlowast

1087906lowastlowast

22561719lowastlowast

042162lowastlowast

Hybrid

s44

11423573lowastlowast

3398933lowastlowast

25697199lowastlowast

20633716lowastlowast

472874lowastlowast

1391526lowastlowast

674123518lowastlowast

261839lowastlowast

Parentsv

ersush

ybrid

s1

43693104lowastlowast

163619

916lowastlowast

3896838

3896

2793lowastlowast

28873741lowastlowast

4272

2798lowastlowast

234144289lowastlowast

5367022lowastlowast

Error

108

281184

1356832

1173684

674337

040104

306

462

2015

1619

005535

E 2

Replicates

2583819

3707389lowast

1596635

198738

034925

448213

899529

007177

Treatm

ents

549997443lowastlowast

2825177lowastlowast

35378177lowastlowast

19074306lowastlowast

665284lowastlowast

1764

081lowastlowast

257694570lowastlowast

2177519lowastlowast

Parents

96507784lowastlowast

3718

879lowastlowast

69866289lowastlowast

1798

5404lowastlowast

705379lowastlowast

1408518lowastlowast

10612519lowastlowast

077429lowastlowast

Hybrid

s44

10569731lowastlowast

2127302lowastlowast

2910

2537lowastlowast

19429240lowastlowast

329256lowastlowast

7000

03lowastlowast

186026552lowastlowast

1483428lowastlowast

Parentsv

ersush

ybrid

s1

16223710lowastlowast

25488361lowastlowast

1113

326

13257362lowastlowast

15089673lowastlowast

5178

3569lowastlowast

5634825791lowastlowast

51618348lowastlowast

Error

108

334760

1097039

1049505

635570

06764

116

0576

694613

006206

E 3

Replicates

2894398

4808815lowast

mdashmdash

051076

095989

3768421

030220

Treatm

ents

541261560

0lowastlowast

4842216lowastlowast

mdashmdash

2811077lowastlowast

5947316lowastlowast

3870844

50lowastlowast

3445076lowastlowast

Parents

97135

735lowastlowast

1097

6388lowastlowast

mdashmdash

1468699lowastlowast

1112

764lowastlowast

136081650lowastlowast

1307836lowastlowast

Hybrid

s44

13217740lowastlowast

1379360

mdashmdash

15540

71lowastlowast

4581632lowastlowast

252084767lowastlowast

2378032lowastlowast

Parentsv

ersush

ybrid

s1

35440

180lowastlowast

102000335lowastlowast

mdashmdash

70200767lowastlowast

109548362lowastlowast

8586095705lowastlowast

69630188lowastlowast

Error

108

782907

1085444

mdashmdash

139771

291325

3019

169

02400

4(b)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Panicle

emergence

Effectiv

etillersplant

Planth

eight

Earlength

Earw

eightp

lant

Dry

fodd

eryieldplant

Grain

yieldplant

Totalbiological

yieldplant

E 1

Replicates

231860

61lowastlowast

416348lowastlowast

1672

9472lowast

1706

411077192

9634539

392777

115544

59Treatm

ents

544152

368lowastlowast

085657lowastlowast

326706089lowastlowast

1602572lowastlowast

6177

0406lowastlowast

304113

204lowastlowast

34285030lowastlowast

575617

582lowastlowast

Parents

94511481lowastlowast

018538lowastlowast

140807476lowastlowast

2240093lowastlowast

11653

959lowastlowast

27014155lowastlowast

3858650lowastlowast

61321919lowastlowast

Hybrid

s44

3201347lowastlowast

100797lowastlowast

110395657lowastlowast

1010

497lowastlowast

40296671lowastlowast

171271411lowastlowast

2413

6880lowastlowast

302999650lowastlowast

Parentsv

ersush

ybrid

s1

4276

5286lowastlowast

02360

9lowast11517452

616lowastlowast

2191

6167lowastlowast

1457662749lowastlowast

8643043524lowastlowast

7546

41053lowastlowast

17199467546lowastlowast

Error

108

468159

004907

3692099

224610

17114

303498126

682350

5075268

E 2

Replicates

2656364

002556

61575565lowastlowast

828842lowastlowast

589094

2268506

260877

1507116

Treatm

ents

542999618lowastlowast

069822lowastlowast

268998723lowastlowast

2436886lowastlowast

60592784lowastlowast

247259877lowastlowast

4192

0073lowastlowast

513483489lowastlowast

Parents

926300

00lowastlowast

048079lowastlowast

165555

405lowastlowast

3871874lowastlowast

1412

2393lowastlowast

19543682lowastlowast

3132

426lowastlowast

57230706lowastlowast

Hybrid

s44

2533

165lowastlowast

07344

9lowastlowast

9616

2857lowastlowast

1463359lowastlowast

35495366lowastlowast

154759288lowastlowast

26262465lowastlowast

293492304lowastlowast

Parentsv

ersush

ybrid

s1

26850135lowastlowast

105929lowastlowast

880476

6697lowastlowast

32357161lowastlowast

1583112746lowastlowast

6366731560lowastlowast

107994

3646lowastlowast

14299370699lowastlowast

Error

108

507598

005829

5234122

149406

955133

2142411

462311

3273886

E 3

Replicates

2029697

021161

9459847

143746

985141

46800

6492

6334

3345160

Treatm

ents

548045499lowastlowast

094511lowastlowast

192420108lowastlowast

3279225lowastlowast

72049211lowastlowast

298953

021lowastlowast

55061308lowastlowast

651732994lowastlowast

Parents

98010

741lowastlowast

094166lowastlowast

116037343lowastlowast

3702185lowastlowast

16305190lowastlowast

2323644

0lowastlowast

4553

893lowastlowast

71484982lowastlowast

Hybrid

s44

6254377lowastlowast

095514lowastlowast

7378

1513lowastlowast

2188348lowastlowast

31813193lowastlowast

190263916lowastlowast

31572179lowastlowast

368689934lowastlowast

Parentsv

ersush

ybrid

s1

8716

7710lowastlowast

053496lowast

609996

3199lowastlowast

47471212lowastlowast

2344

130203lowastlowast

756272

2861lowastlowast

154314

9707lowastlowast

18327859

719lowastlowast

Error

108

696981

007883

5578505

228608

1491014

2574

223

449588

5187705

lowast

Sign

ificant

at119875=005

lowastlowastSign

ificant

at119875=001

14 Advances in Agriculture

Table 5 Mean performance of the genotypes for membrane thermostability in two environments

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594

Advances in Agriculture 15

Table 5 Continued

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434

Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash

lowastValues are transformed values

0

6

12

18

24

30

36

42

48

54

minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity

G73-10796AC-93CVJ-2-5-3-1-3

Togo-II99HS-18

H77833-2H7729-2772451305

H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305

1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305

H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305

77245 times 1305

77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305

77371 times BSECT CP-1

H77833-2 times G73-107

Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits

moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling

survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The

16 Advances in Agriculture

Table6Po

oled

correla

tioncoeffi

cientamon

gvario

uscharactersin

threee

nviro

nments

Characters

Germination

Rateof

emergence

STI

SSTI

Leaves

seedlin

gSeedlin

gheight

Fresh

weight

Dry

weight

MTS

Panicle

emergence

Effectiv

etillersplant

Plant

height

Earlength

Ear

weightp

lant

Dry

fodd

eryield

plant

Grain

yield

plant

Total

biological

yield

plant

Germination

1000

0357lowastlowast

0353lowastlowast

0590lowastlowast

0230

0373lowastlowast

0293lowast

0260

0276lowastminus0135minus0478lowastlowast

0326lowast

minus0069minus0104

minus0190

minus0145

minus0167

Rateof

emergence

1000

0233

0351lowastlowast

0605lowastlowast

0585lowastlowast

0551lowastlowast

0524lowastlowast

0139minus0593lowastlowast

0032

0517lowastlowast

0217

0388lowastlowast

0361lowastlowast

0348lowastlowast

0377lowastlowast

STI

1000

0837lowastlowast

0241

0129

0081

004

80890lowastlowast

minus0215

minus0211

0030minus0007minus0043

minus0101

minus0111

minus0084

SSTI

1000

0233

0264

0183

0140

0745lowastlowast

minus0247minus0268lowast

0184minus0017minus006

6minus0129

minus0144

minus0112

Num

bero

fleaves

1000

0683lowastlowast

0633lowastlowast

060

4lowastlowast

0176minus0474lowastlowast

0109

0576lowastlowast

0496lowastlowast

064

1lowastlowast

0607lowastlowast

0630lowastlowast

0630lowastlowast

Seedlin

gheight

1000

0703lowastlowast

0663lowastlowast

0031minus0493lowastlowast

minus0115

0735lowastlowast

0360lowastlowast

0526lowastlowast

0520lowastlowast

0515lowastlowast

0532lowastlowast

Freshweight

1000

0957lowastlowast

minus0016

0425lowastlowast

minus0082

0661lowastlowast

0351lowastlowast

0581lowastlowast

0496lowastlowast

0576lowastlowast

0533lowastlowast

Dry

weight

1000minus0033minus0428lowastlowast

minus0016

0634lowastlowast

0354lowastlowast

0620lowastlowast

0509lowastlowast

0614lowastlowast

0554lowastlowast

MTS

1000minus040

minus0177minus0037

0015

minus0074

minus0138

minus0131

minus0120

Panicle

emergence

1000

minus0150minus0269lowast

0022minus0329lowast

minus0337lowast

minus0280lowast

minus0341lowast

Effectiv

etillersplant

1000

minus0012

0170

0366lowastlowast

040

5lowastlowast

0323lowast

0401lowastlowast

Planth

eight

1000

0650lowastlowast

0603lowastlowast

0596lowastlowast

0619lowastlowast

0610lowastlowast

Earlength

1000

0608lowastlowast

0671lowastlowast

064

8lowastlowast

066

4lowastlowast

Earw

eightp

lant

1000

0910lowastlowast

0984lowastlowast

0957lowastlowast

Dry

fodd

eryield

plant

1000

0907lowastlowast

0991lowastlowast

Grain

yield

plant

1000

0950lowastlowast

Totalbiological

yield

plant

1000

lowastSign

ificant

at119875=005lowastlowastSign

ificant

at119875=001

Advances in Agriculture 17

faster germination and early vigour in seedling could act asphysical indicators for heat tolerance

Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits

Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics

Conflict of Interests

The authors declare that there is no conflict of interestsregarding the publication of this paper

References

[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981

[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993

[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006

[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993

[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013

[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007

[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965

[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011

[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001

[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009

[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962

[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967

[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963

[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001

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GenomicsInternational Journal of

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Page 8: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

8 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

777371timesBS

ECT

CP-1

1377

1487

1892

1585

928

2660

3330

2306

150

258

301

236

5733

4533

4700

4989

242

220

293

252

1513

814550

1315

014279

896AC

-93

1303

1259

1852

1471

1056

1445

5972

2825

185

147

586

306

5500

4833

3833

4722

256

304

318

293

15577

14032

10000

13203

9To

go-II

1056

1220

2272

1516

924

1466

9113

3834

146

129

883

386

6167

4533

4600

5100

185

149

124

153

14578

12073

10033

12228

1099HS-18

1277

1075

1808

1387

1241

2833

7070

3715

228

226

615

356

6100

5000

4167

5089

243

173

324

247

12627

10355

1017

311052

1H77833-2times

H7729-2

1432

1498

2233

1721

1350

4405

8373

4709

159

499

782

480

5767

4433

3800

4667309

290

248

282

21010

18071

15847

18309

2H77833-2times

G73-107

1675

1635

2205

1838

1236

3211

5789

3412

134

313

547

331

5833

4767

3833

4811

262

295

236

265

21253

20449

15808

1917

0

3H77833-2times

77245

1577

1522

2263

1787

1058

6933

8719

5570

255

553

782

530

5267

4633

4600

4833

273

201

265

246

2074

119511

15208

18487

4H77833-2times

CVJ-2-5-3-1-3

1791

1757

2165

1904

20218030

5276

5109

354

779

477

537

5533

4233

3767

4511

165

330

351

282

21827

20072

17110

19670

5H77833-2times1305

1700

1842

28412128

1427

6228

9134

5596

570

579

813

654

5800

4267

3333

4467340

426

451

406

19816

19444

16037

18432

6H77833-2times

(77371times

BSEC

TCP

-1)

1453

1515

3120

2029

789

465510428

5291

211

408

858

492

5233

4167

3267

4222

203

283

267

251

20677

1913

816470

1876

2

7H77833-2times

96AC

-93

1835

1783

2427

2015

2532

6649

1610

684

29

350

650

1473

824

5467

4233

3700

4467243

239

256

246

22278

20434

15735

19482

8H77833-2times

Togo-II

1564

15632500

1876

1882

4772

11660

6105

296

469

972

579

5667

4533

440048

67

169

260

211

213

2412

82375

619389

22424

9H77833-2times

99HS-18

159318002049

1814

1571

480214024

6799

203

544

1547

765

6033

460040

0048

78

261

225

256

247

23466

21081

1793

320

827

10H7729-2times

G73-107

14951568

2075

1713

959

5157

8517

4877

218

382

666

422

6133

5067

4833

5344

300

273

239

271

23651

2191

717083

20884

11H7729-2times77245

1557

1537

2613

1902

1851

5756

5729

4445366

562

524

484

5633

4800

4633

5022

133

233

297

221

1979

219914

15250

18319

12H7729-2times

CVJ-2-5-3-1-3

18442033

28932257

1651

806114885

8199

264

597

1054

638

6333

4600

4233

5056

170

211

217

199

24629

23339

1970

022556

13H7729-2times1305

1325

1677309920

34

1756

724314048

7682

318

635

1232

728

630046

67

3633

4867298

261

361

307

21666

19371

17422

19486

14H7729-2times(77371

timesBS

ECTCP

-1)

1958

1887

3105

2317

1118

4675

14411

6734

242

457

1318

672

5900

4500

3800

4733

164

209

240

204

23661

1995

419250

20955

15H7729-2times

96AC

-93

1512

1717

2779

2002266844

08

11393

6156

356

500

1211

689

5633

4533

4200

4789

221

195

282

232

2194

921635

17213

20266

Advances in Agriculture 9

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

16H7729-2times

Togo-II

1519

1738

2159

1805

2437

9867

1616

294

89

361

961

1527

950

6167

4700

4200

5022

234

272

230

246

26338

2414

520553

23678

17H7729-2times

99HS-18

1938

1787

3151

2292

1574

6218

11318

6370

290

606

956

617

6067

4767

406749

67

172

210

199

194

24689

21854

1778

921444

18G73-107times77245

1856

1813

2210

1960

2435

8550

7828

6271338

633

612

528

5500

4633

440048

44

199

255

307

254

1993

619277

16095

18436

19G73-107times

CVJ-2-5-3-1-3

1517

1769

2407

1897

1556

7728

9136

6140

239

876

917

677

5900

4300

3400

4533

233

266

315

272

23418

22333

19317

21689

20G73-107times1305

1217

1507

2832

1852

1777

5189

1114

960

38

289

588

1197

691

6733

5000

4233

5322

286

210

312

269

2078

81992

817067

19261

21G73-107times(77371

timesBS

ECTCP

-1)

18551946

2730

2177

1801

3482

10415

5233

239

277

753

423

5500

4467

3433

4467243

197

273

238

23817

20263

17425

20502

22G73-107times

96AC

-93

1718

1840

28612140

1345

9333

14669

8449

297

845

1278

807

5600

5033

4633

5089

270

243

300

271

28537

21461

16875

22291

23G73-107timesTo

go-II

168018083087

2192

1112

4678

9319

5036

186

473

985

548

6033

4767

4200

5000

210

209

231

217

24864

24609

21625

23699

24G73-107times99HS-18

1398

1675

3370

2148

2753

8672

11918

7781

362

691

981

678

566746

00

3500

4589

170

247

270

229

24677

20567

17855

21033

2577245times

CVJ-2-5-3-1-3

1500

1728

2203

1810

2282

4679

11492

6151

328

424

1116

623

6000

4333

420048

44300

231

370

300

21257

20497

1772

519826

2677245times1305

143815772080

1698

949

10439

12053

7814

121

915

992

676

5767

4800

3500

4689

168246

316

244

1978

91779

015517

17699

2777245times(77371times

BSEC

TCP

-1)

150516972846

2016

3222

8518

12621

8120

469

827

1141

812

5400

4367

3833

4533

196

225

242

221

2092

819485

1719

219202

2877245times96AC

-93

1653

1797

2117

1856

1934

6056

11543

6511

352

563

911

608

5333

4267

3833

4478

186

169

198

184

22003

19054

1676

719275

2977245timesTo

go-II

1723

1718

3163

2201

1749

8250

15443

8481229

806

1412

816

5967

4700

4067

4911

174

245

298

239

22075

23503

19875

21818

3077245times99HS-18

2016

1970

28542280

3217

13027

3531

6591395

965

285

549

5733

4467

3833

4678

148

228

209

195

2212

721462

1690

020

163

31CV

J-2-5-3-1-3times

1305

1358

1422

2179

1653

1254

2803

1019

44750

178

254

801

411

6033

4467

3367

4622

312

193

330

278

22269

19547

17250

19689

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

1335

1742

2400

1826

922

5226

12806

6318

236

458

1054

583

5633

4433

3600

4556

321

315

259

298

2210

419524

17087

19572

33CV

J-2-5-3-1-3times

96AC

-93

1552

16803260

2164

166710094

11941

7901250

974

1115

779

5533

4533

446748

44

170

202

317

230

2392

123317

19333

2219

0

34CV

J-2-5-3-1-3times

Togo-II

142216072638

1889

1012

4163

11061

5412

261

377

891

510

6167

4967

4533

5222

207

225

325

252

23590

22860

20278

22243

35CV

J-2-5-3-1-3times

99HS-18

1435

1600

2700

1912

3056

3237

7089

4461449

301

658

469

5700

44673800

4656

148

178

225

183

22444

20240

1696

719884

361305times(77371times

BSEC

TCP

-1)

1499

1767

2058

1774

1882

4665

7920

4822

356

426

724

502

566742673800

4578

271

242

305

273

22011

19295

16417

19241

371305times96AC

-93

16781605

2757

2014

1516

10037

11960

7838

311

11381284

911

560040

673400

4356

182

276

251

236

2396

519625

15800

1979

738

1305timesTo

go-II

1177

16802249

1702

902

4676

9388

4989

241

456

795

497

6533

5533

4733

5600

214

266

296

259

21894

20217

19222

20444

391305times99HS-18

1774

1767

2766

2102

2145

4628

10618

5797

301

447

991

580

5933

4500

3733

4722

128

142

180

150

2275

719413

16967

1971

2

40(77371times

BSEC

TCP

-1)times

96AC

-93

1748

1795

31712238

1537

12586

14379

9501

211

1104

1183

833

546744

00346744

44

127

201

226

185

23294

21089

17858

2074

7

10 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(

days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

41(77371times

BSEC

TCP

-1)times

Togo-II

19652007

2653

2208

2136

5601

9503

5747

315

635

1001

650

5533

4833

410048

22

213

241

286

247

22662

22094

17667

20808

42(77371times

BSEC

TCP

-1)times

99HS-18

15011687

2705

1964

1935

7253

11806

6998

314

704

1067

695

5467

4433

5133

5011

177

167

175

173

22305

19277

16392

19325

4396AC

-93timesTo

go-II1759

1618

2123

1834

1539

10347

12212

8033

325

907

1090

774

5900

450039674789

214

269

339

274

23252

21554

16875

20560

4496AC

-93times

99HS-18

1353

1618

2925

1966

3532

7223

11950

7569

440

654

1024

706

5600

4900

4533

5011

149

175

206

177

24494

20698

18111

2110

145

Togo-IItimes

99HS-18

1117

1278

2582

1659

3448

6764

11910

7374

477

767

1207

817

6000

5133

4333

5156

183

203

216

201

17683

15541

14333

15853

Mean

1513

1618

2492

1874

1656

5773

9854

5761

272

538

885

565

586846

534116

4879

218

233

268

240

21297

19547

16529

1912

4SE

101

073

098

mdash079

152

317

mdash014

014

028

mdash351

365

428

mdash036

039

046

mdash985

1173

1211

mdashCD

284

205

276

mdash221

427

890

mdash038

040

079

mdash12

513

015

2mdash

013

014

016

mdash351

418

431

mdash

(c)

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

1642

1667

1744

1684

1504

1349

1526

1460

2783

2405

2634

2607

836

745

908

830

4287

3754

4160

4067

2H7729-2

1693

1817

1950

1820

1012

859

1016

962

3433

2809

2591

2944

649

597

651

632

4445

3668

3607

3906

3G73-107

2000

2028

2077

2035

2280

1966

2312

2186

4695

4023

4229

4316

1281

1170

1463

1305

6975

5989

6541

6502

477245

1523

1630

1728

1627

1796

2489

2702

2329

5423

4851

4498

4924

748

1464

1743

1319

7219

7341

7200

7253

5CV

J-2-5-3-1-3

2282

2692

2787

2587

2411

2643

2901

2652

5857

4836

5024

5239

1206

1335

1769

1437

8268

7479

7925

7891

61305

1851

1951

2054

1952

2026

1763

1908

1899

4657

4414

4463

4511

1468

1336

1539

1448

6683

6177

6371

6410

777371timesBS

ECTCP

-11629

1645

1733

1669

1244

1692

1884

1607

4925

4183

4435

4515

625

1092

1483

1067

6169

5875

6319

6121

896AC

-93

1379

1519

1600

1499

2066

2942

3088

2698

4501

4095

4229

4275

984

1565

1678

1409

6567

7037

7316

6973

9To

go-II

1904

2057

2103

2021

2167

1844

2358

2123

4520

3446

3904

3957

1298

1138

1502

1313

6687

5290

6262

6080

1099HS-18

1475

1485

1633

1531

3180

2904

3382

3155

5633

4139

5205

4992

1656

1536

1879

1690

8813

7043

8587

8147

1H77833-2timesH7729-2

2010

2046

2133

2063

3645

3075

4377

3699

7675

6540

7199

7138

2014

1708

2880

2201

11320

9615

11576

10837

2H77833-2timesG73-107

2066

2101

2122

2096

5002

4053

4601

4552

7882

7430

6963

7425

3146

2565

2808

2840

12884

11482

11564

11977

3H77833-2times77245

1764

2048

2083

1965

3769

4497

4559

4275

8681

8515

8475

8557

2284

3356

3377

3006

12450

13012

13034

12832

4H77833-2times

CVJ-2-5-3-1-3

2205

2383

2733

2441

3693

5655

5220

4856

11656

10287

1010

510683

2198

4488

4176

3621

15348

1594

215325

15538

5H77833-2times1305

2209

2423

2491

2374

6008

5842

6933

6261

15238

14311

13889

14479

3758

4057

5591

4469

21246

2015

320822

2074

0

6H77833-2times(77371times

BSEC

TCP

-1)

1789

1823

1992

1868

3593

4288

4903

4262

8922

6522

7429

7625

2178

3224

3070

2824

12515

10811

12333

11886

7H77833-2times96AC

-93

1739

1960

2011

1903

4557

4350

4818

4575

7916

6068

7290

7092

2411

2529

3191

2710

12474

10419

1210

811667

8H77833-2timesTo

go-II

1927

2389

2406

2241

4185

5828

6215

5409

12319

10279

11023

11207

2433

4254

4631

3773

16504

1610

717238

16616

9H77833-2times99HS-18

1941

2245

2330

2172

4227

4139

4529

4298

9211

8120

7969

8433

3154

3184

3253

3197

13438

12259

12498

1273

210

H7729-2timesG73-107

1968

2109

2158

2079

3725

3042

3488

3418

7313

5956

6174

6481

2822

2001

2357

2393

11038

8998

9662

9899

Advances in Agriculture 11

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

11H7729-2times77245

1847

2048

2200

2032

2467

3759

5466

3898

7672

7449

8921

8014

1356

2350

3796

2500

1013

911208

14387

11911

12H7729-2times

CVJ-2-5-3-1-3

2218

2585

2767

2523

3674

4372

4483

4176

10053

9467

8664

9395

2417

3312

3345

3025

1372

813839

1314

713571

13H7729-2times1305

2169

2338

2547

2352

6728

6166

6792

6562

13333

11662

13301

1276

54128

4501

5660

4763

20061

17828

20093

19327

14H7729-2times(77371times

BSEC

TCP

-1)

1925

2095

2127

2049

3530

4165

4649

4115

9638

6917

7698

8084

1681

2833

3150

2555

1316

8110

82

12347

1219

915

H7729-2times96AC

-93

2012

2070

2180

2087

4955

4447

4596

4666

8738

6882

7762

7794

3754

3558

3181

3498

13693

11329

12359

12460

16H7729-2timesTo

go-II

2138

2428

2563

2377

4949

4660

5118

4909

8549

7243

7191

7661

3586

3353

3028

3322

13498

11903

12310

12570

17H7729-2times99HS-18

1899

1933

2073

1969

4046

4527

4988

4520

8492

7895

8144

8177

2561

3378

3553

3164

12539

12422

1313

212698

18G73-107times77245

1840

2253

2437

2177

2580

4776

5915

4424

11673

10608

10459

10913

1358

3163

4286

2936

14253

15385

16374

15337

19G73-107times

CVJ-2-5-3-1-3

2324

2537

2691

2517

4958

5030

5599

5196

9524

9395

1114

610022

3241

3645

4444

3776

14483

14425

1674

515217

20G73-107times1305

2228

2433

2583

2415

6340

5794

6373

6169

12490

1015

8119

61

11536

4199

4199

5018

4472

18830

1595

218334

1770

6

21G73-107times(77371times

BSEC

TCP

-1)

1943

2128

2220

2097

3936

3980

5770

4562

11697

9858

10303

10619

1874

2369

4181

2808

15633

13839

16073

1518

222

G73-107times96AC

-93

2118

2218

2317

2217

4270

5843

6201

5438

10319

9872

1015

910117

2703

4460

4379

3847

14589

15715

16359

15555

23G73-107timesTo

go-II

2237

2365

2733

2445

5736

5175

6822

5911

12098

10808

1299

611967

3984

3670

5372

4342

17835

1598

219818

17878

24G73-107times99HS-18

2025

2081

2322

2143

3558

4229

6612

4800

12350

9685

11516

1118

42196

2782

5009

3329

1590

813914

1812

915983

2577245timesCV

J-2-5-3-1-3

2109

2360

2675

2381

6760

6558

7299

6872

15225

11861

1416

21374

94225

4752

5839

4939

2198

518418

21461

20621

2677245times1305

1919

2100

2197

2072

5700

5142

5347

5397

11687

10565

10737

10996

4318

3956

4244

4173

17386

1570

816085

16393

2777245times(77371times

BSEC

TCP

-1)

1851

1935

2020

1936

2577

2460

2838

2625

6989

4778

4636

5468

1631

1662

1999

1764

9566

7238

7474

8093

2877245times96AC

-93

1729

1780

2133

1881

5541

5058

5748

5449

11952

8174

9526

9884

3646

3587

4071

3768

17493

13232

15274

15333

2977245timesTo

go-II

2300

2508

2658

2489

3067

5431

5963

4820

11737

11021

1216

411641

1598

4053

4770

3474

14805

16452

1812

616461

3077245times99HS-18

1982

2019

2223

2075

3846

3119

4225

3730

7493

6720

6476

6896

3205

2578

2643

2809

11339

9839

1070

110626

12 Advances in Agriculture

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

31CV

J-2-5-3-1-3times1305

2406

2553

3067

2675

4178

4796

6281

5085

13822

11425

12252

12500

2472

3241

4907

3540

18000

16222

18532

17585

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

2142

2173

2238

2184

4522

5235

5640

5132

11673

9658

10248

10526

2614

3766

4178

3519

1619

514893

15888

15659

33CV

J-2-5-3-1-3times

96AC

-93

2350

2477

2600

2476

4676

4280

6090

5015

11975

9832

10868

10891

3097

2931

4445

3491

16651

14111

1695

815907

34CV

J-2-5-3-1-3times

Togo-II

2093

2666

2989

2583

4058

3727

5190

4325

12588

10717

11217

11507

2742

2570

4185

3166

16645

14443

16407

15832

35CV

J-2-5-3-1-3times

99HS-18

2028

2311

2386

2242

3287

2513

5356

3719

10055

9733

1036

410051

2004

1581

4251

2612

13342

12246

1572

01376

9

361305times(77371times

BSEC

TCP

-1)

2005

2173

2210

2129

3569

4692

5140

4467

11652

9826

10041

10507

2005

3665

4179

3283

15221

14518

1518

214974

371305times96AC

-93

1822

2003

2047

1957

5115

4023

4813

4650

9965

7675

7571

8404

3456

2647

3155

3086

15080

11698

12384

13054

381305timesTo

go-II

2545

2583

2644

2591

6885

7283

6795

6987

12265

11410

12583

12086

4500

6120

5350

5323

1915

018693

19378

19074

391305times99HS-18

2070

2408

2593

2357

5219

4332

4800

4784

1092

97677

8968

9192

3924

3332

3840

3699

1614

912009

1376

913976

40(77371times

BSEC

TCP

-1)

times96AC

-93

2056

2115

2157

2109

3158

4463

4717

4113

9166

7540

8100

8268

1880

3542

3347

2923

12324

12003

12817

12381

41(77371times

BSEC

TCP

-1)

timesTo

go-II

1868

2253

2450

2190

4422

6458

7038

5973

1415

312369

1379

713439

2291

4646

5586

4174

18575

18827

20835

19412

42(77371times

BSEC

TCP

-1)

times99HS-18

1995

2026

2209

2077

2720

2909

3543

3057

7392

4692

5023

5702

1766

2187

2147

2033

10112

7601

8566

8760

4396AC

-93timesTo

go-II

1953

2027

2511

2164

5972

4391

7126

5830

15537

1392

714432

14632

3780

2538

5655

3991

21510

18318

21558

20462

4496AC

-93times99HS-18

1952

2021

2242

2072

3465

2758

4496

3573

5959

5186

6094

5746

2707

2154

2409

2423

9424

7944

10590

9319

45To

go-IItimes

99HS-18

1932

2008

2658

2199

5381

4995

5436

5270

10299

8876

9244

9473

3986

3842

3668

3832

15680

13871

14680

1474

3Mean

1982

2146

2301

2143

3963

4123

4836

4307

9498

8087

8663

8749

2510

2914

3513

2979

13460

12210

13499

13056

SE243

198

245

mdash671

501

626

mdash959

750

822

mdash423

349

344

mdash1155

927

1167

mdashCD

087

071

087

mdash239

178

223

mdash342

267

293

mdash15

112

412

2mdash

411

330

416

mdash

Advances in Agriculture 13Ta

ble4Analysis

ofvaria

ncefor

10times10

half-diallelfor

different

charactersin

threee

nviro

nments

(a)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Germination

()

Rateof

emergence

STI()

SSTI

()

Num

bero

fleavesseedling

Seedlin

gheight

(cm)

Freshweight

(g)seedlin

gDry

weight

(g)seedlin

g

E 1

Replicates

2652731

596768

783393

20700

93018674

33764

1033999

002590

Treatm

ents

541097

0255lowastlowast

8181153lowastlowast

30863092lowastlowast

20680900lowastlowast

1100708lowastlowast

2106317lowastlowast

930829527lowastlowast

319767lowastlowast

Parents

95118

163lowastlowast

14289925lowastlowast

59114816lowastlowast

18880254lowastlowast

1084227lowastlowast

1087906lowastlowast

22561719lowastlowast

042162lowastlowast

Hybrid

s44

11423573lowastlowast

3398933lowastlowast

25697199lowastlowast

20633716lowastlowast

472874lowastlowast

1391526lowastlowast

674123518lowastlowast

261839lowastlowast

Parentsv

ersush

ybrid

s1

43693104lowastlowast

163619

916lowastlowast

3896838

3896

2793lowastlowast

28873741lowastlowast

4272

2798lowastlowast

234144289lowastlowast

5367022lowastlowast

Error

108

281184

1356832

1173684

674337

040104

306

462

2015

1619

005535

E 2

Replicates

2583819

3707389lowast

1596635

198738

034925

448213

899529

007177

Treatm

ents

549997443lowastlowast

2825177lowastlowast

35378177lowastlowast

19074306lowastlowast

665284lowastlowast

1764

081lowastlowast

257694570lowastlowast

2177519lowastlowast

Parents

96507784lowastlowast

3718

879lowastlowast

69866289lowastlowast

1798

5404lowastlowast

705379lowastlowast

1408518lowastlowast

10612519lowastlowast

077429lowastlowast

Hybrid

s44

10569731lowastlowast

2127302lowastlowast

2910

2537lowastlowast

19429240lowastlowast

329256lowastlowast

7000

03lowastlowast

186026552lowastlowast

1483428lowastlowast

Parentsv

ersush

ybrid

s1

16223710lowastlowast

25488361lowastlowast

1113

326

13257362lowastlowast

15089673lowastlowast

5178

3569lowastlowast

5634825791lowastlowast

51618348lowastlowast

Error

108

334760

1097039

1049505

635570

06764

116

0576

694613

006206

E 3

Replicates

2894398

4808815lowast

mdashmdash

051076

095989

3768421

030220

Treatm

ents

541261560

0lowastlowast

4842216lowastlowast

mdashmdash

2811077lowastlowast

5947316lowastlowast

3870844

50lowastlowast

3445076lowastlowast

Parents

97135

735lowastlowast

1097

6388lowastlowast

mdashmdash

1468699lowastlowast

1112

764lowastlowast

136081650lowastlowast

1307836lowastlowast

Hybrid

s44

13217740lowastlowast

1379360

mdashmdash

15540

71lowastlowast

4581632lowastlowast

252084767lowastlowast

2378032lowastlowast

Parentsv

ersush

ybrid

s1

35440

180lowastlowast

102000335lowastlowast

mdashmdash

70200767lowastlowast

109548362lowastlowast

8586095705lowastlowast

69630188lowastlowast

Error

108

782907

1085444

mdashmdash

139771

291325

3019

169

02400

4(b)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Panicle

emergence

Effectiv

etillersplant

Planth

eight

Earlength

Earw

eightp

lant

Dry

fodd

eryieldplant

Grain

yieldplant

Totalbiological

yieldplant

E 1

Replicates

231860

61lowastlowast

416348lowastlowast

1672

9472lowast

1706

411077192

9634539

392777

115544

59Treatm

ents

544152

368lowastlowast

085657lowastlowast

326706089lowastlowast

1602572lowastlowast

6177

0406lowastlowast

304113

204lowastlowast

34285030lowastlowast

575617

582lowastlowast

Parents

94511481lowastlowast

018538lowastlowast

140807476lowastlowast

2240093lowastlowast

11653

959lowastlowast

27014155lowastlowast

3858650lowastlowast

61321919lowastlowast

Hybrid

s44

3201347lowastlowast

100797lowastlowast

110395657lowastlowast

1010

497lowastlowast

40296671lowastlowast

171271411lowastlowast

2413

6880lowastlowast

302999650lowastlowast

Parentsv

ersush

ybrid

s1

4276

5286lowastlowast

02360

9lowast11517452

616lowastlowast

2191

6167lowastlowast

1457662749lowastlowast

8643043524lowastlowast

7546

41053lowastlowast

17199467546lowastlowast

Error

108

468159

004907

3692099

224610

17114

303498126

682350

5075268

E 2

Replicates

2656364

002556

61575565lowastlowast

828842lowastlowast

589094

2268506

260877

1507116

Treatm

ents

542999618lowastlowast

069822lowastlowast

268998723lowastlowast

2436886lowastlowast

60592784lowastlowast

247259877lowastlowast

4192

0073lowastlowast

513483489lowastlowast

Parents

926300

00lowastlowast

048079lowastlowast

165555

405lowastlowast

3871874lowastlowast

1412

2393lowastlowast

19543682lowastlowast

3132

426lowastlowast

57230706lowastlowast

Hybrid

s44

2533

165lowastlowast

07344

9lowastlowast

9616

2857lowastlowast

1463359lowastlowast

35495366lowastlowast

154759288lowastlowast

26262465lowastlowast

293492304lowastlowast

Parentsv

ersush

ybrid

s1

26850135lowastlowast

105929lowastlowast

880476

6697lowastlowast

32357161lowastlowast

1583112746lowastlowast

6366731560lowastlowast

107994

3646lowastlowast

14299370699lowastlowast

Error

108

507598

005829

5234122

149406

955133

2142411

462311

3273886

E 3

Replicates

2029697

021161

9459847

143746

985141

46800

6492

6334

3345160

Treatm

ents

548045499lowastlowast

094511lowastlowast

192420108lowastlowast

3279225lowastlowast

72049211lowastlowast

298953

021lowastlowast

55061308lowastlowast

651732994lowastlowast

Parents

98010

741lowastlowast

094166lowastlowast

116037343lowastlowast

3702185lowastlowast

16305190lowastlowast

2323644

0lowastlowast

4553

893lowastlowast

71484982lowastlowast

Hybrid

s44

6254377lowastlowast

095514lowastlowast

7378

1513lowastlowast

2188348lowastlowast

31813193lowastlowast

190263916lowastlowast

31572179lowastlowast

368689934lowastlowast

Parentsv

ersush

ybrid

s1

8716

7710lowastlowast

053496lowast

609996

3199lowastlowast

47471212lowastlowast

2344

130203lowastlowast

756272

2861lowastlowast

154314

9707lowastlowast

18327859

719lowastlowast

Error

108

696981

007883

5578505

228608

1491014

2574

223

449588

5187705

lowast

Sign

ificant

at119875=005

lowastlowastSign

ificant

at119875=001

14 Advances in Agriculture

Table 5 Mean performance of the genotypes for membrane thermostability in two environments

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594

Advances in Agriculture 15

Table 5 Continued

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434

Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash

lowastValues are transformed values

0

6

12

18

24

30

36

42

48

54

minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity

G73-10796AC-93CVJ-2-5-3-1-3

Togo-II99HS-18

H77833-2H7729-2772451305

H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305

1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305

H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305

77245 times 1305

77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305

77371 times BSECT CP-1

H77833-2 times G73-107

Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits

moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling

survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The

16 Advances in Agriculture

Table6Po

oled

correla

tioncoeffi

cientamon

gvario

uscharactersin

threee

nviro

nments

Characters

Germination

Rateof

emergence

STI

SSTI

Leaves

seedlin

gSeedlin

gheight

Fresh

weight

Dry

weight

MTS

Panicle

emergence

Effectiv

etillersplant

Plant

height

Earlength

Ear

weightp

lant

Dry

fodd

eryield

plant

Grain

yield

plant

Total

biological

yield

plant

Germination

1000

0357lowastlowast

0353lowastlowast

0590lowastlowast

0230

0373lowastlowast

0293lowast

0260

0276lowastminus0135minus0478lowastlowast

0326lowast

minus0069minus0104

minus0190

minus0145

minus0167

Rateof

emergence

1000

0233

0351lowastlowast

0605lowastlowast

0585lowastlowast

0551lowastlowast

0524lowastlowast

0139minus0593lowastlowast

0032

0517lowastlowast

0217

0388lowastlowast

0361lowastlowast

0348lowastlowast

0377lowastlowast

STI

1000

0837lowastlowast

0241

0129

0081

004

80890lowastlowast

minus0215

minus0211

0030minus0007minus0043

minus0101

minus0111

minus0084

SSTI

1000

0233

0264

0183

0140

0745lowastlowast

minus0247minus0268lowast

0184minus0017minus006

6minus0129

minus0144

minus0112

Num

bero

fleaves

1000

0683lowastlowast

0633lowastlowast

060

4lowastlowast

0176minus0474lowastlowast

0109

0576lowastlowast

0496lowastlowast

064

1lowastlowast

0607lowastlowast

0630lowastlowast

0630lowastlowast

Seedlin

gheight

1000

0703lowastlowast

0663lowastlowast

0031minus0493lowastlowast

minus0115

0735lowastlowast

0360lowastlowast

0526lowastlowast

0520lowastlowast

0515lowastlowast

0532lowastlowast

Freshweight

1000

0957lowastlowast

minus0016

0425lowastlowast

minus0082

0661lowastlowast

0351lowastlowast

0581lowastlowast

0496lowastlowast

0576lowastlowast

0533lowastlowast

Dry

weight

1000minus0033minus0428lowastlowast

minus0016

0634lowastlowast

0354lowastlowast

0620lowastlowast

0509lowastlowast

0614lowastlowast

0554lowastlowast

MTS

1000minus040

minus0177minus0037

0015

minus0074

minus0138

minus0131

minus0120

Panicle

emergence

1000

minus0150minus0269lowast

0022minus0329lowast

minus0337lowast

minus0280lowast

minus0341lowast

Effectiv

etillersplant

1000

minus0012

0170

0366lowastlowast

040

5lowastlowast

0323lowast

0401lowastlowast

Planth

eight

1000

0650lowastlowast

0603lowastlowast

0596lowastlowast

0619lowastlowast

0610lowastlowast

Earlength

1000

0608lowastlowast

0671lowastlowast

064

8lowastlowast

066

4lowastlowast

Earw

eightp

lant

1000

0910lowastlowast

0984lowastlowast

0957lowastlowast

Dry

fodd

eryield

plant

1000

0907lowastlowast

0991lowastlowast

Grain

yield

plant

1000

0950lowastlowast

Totalbiological

yield

plant

1000

lowastSign

ificant

at119875=005lowastlowastSign

ificant

at119875=001

Advances in Agriculture 17

faster germination and early vigour in seedling could act asphysical indicators for heat tolerance

Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits

Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics

Conflict of Interests

The authors declare that there is no conflict of interestsregarding the publication of this paper

References

[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981

[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993

[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006

[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993

[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013

[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007

[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965

[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011

[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001

[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009

[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962

[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967

[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963

[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001

Submit your manuscripts athttpwwwhindawicom

Nutrition and Metabolism

Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

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Agronomy

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Microbiology

The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom

Applied ampEnvironmentalSoil Science

Volume 2014

AgricultureAdvances in

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

BiodiversityInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

GenomicsInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Plant GenomicsInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Biotechnology Research International

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Forestry ResearchInternational Journal of

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Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

EcologyInternational Journal of

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Page 9: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

Advances in Agriculture 9

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

16H7729-2times

Togo-II

1519

1738

2159

1805

2437

9867

1616

294

89

361

961

1527

950

6167

4700

4200

5022

234

272

230

246

26338

2414

520553

23678

17H7729-2times

99HS-18

1938

1787

3151

2292

1574

6218

11318

6370

290

606

956

617

6067

4767

406749

67

172

210

199

194

24689

21854

1778

921444

18G73-107times77245

1856

1813

2210

1960

2435

8550

7828

6271338

633

612

528

5500

4633

440048

44

199

255

307

254

1993

619277

16095

18436

19G73-107times

CVJ-2-5-3-1-3

1517

1769

2407

1897

1556

7728

9136

6140

239

876

917

677

5900

4300

3400

4533

233

266

315

272

23418

22333

19317

21689

20G73-107times1305

1217

1507

2832

1852

1777

5189

1114

960

38

289

588

1197

691

6733

5000

4233

5322

286

210

312

269

2078

81992

817067

19261

21G73-107times(77371

timesBS

ECTCP

-1)

18551946

2730

2177

1801

3482

10415

5233

239

277

753

423

5500

4467

3433

4467243

197

273

238

23817

20263

17425

20502

22G73-107times

96AC

-93

1718

1840

28612140

1345

9333

14669

8449

297

845

1278

807

5600

5033

4633

5089

270

243

300

271

28537

21461

16875

22291

23G73-107timesTo

go-II

168018083087

2192

1112

4678

9319

5036

186

473

985

548

6033

4767

4200

5000

210

209

231

217

24864

24609

21625

23699

24G73-107times99HS-18

1398

1675

3370

2148

2753

8672

11918

7781

362

691

981

678

566746

00

3500

4589

170

247

270

229

24677

20567

17855

21033

2577245times

CVJ-2-5-3-1-3

1500

1728

2203

1810

2282

4679

11492

6151

328

424

1116

623

6000

4333

420048

44300

231

370

300

21257

20497

1772

519826

2677245times1305

143815772080

1698

949

10439

12053

7814

121

915

992

676

5767

4800

3500

4689

168246

316

244

1978

91779

015517

17699

2777245times(77371times

BSEC

TCP

-1)

150516972846

2016

3222

8518

12621

8120

469

827

1141

812

5400

4367

3833

4533

196

225

242

221

2092

819485

1719

219202

2877245times96AC

-93

1653

1797

2117

1856

1934

6056

11543

6511

352

563

911

608

5333

4267

3833

4478

186

169

198

184

22003

19054

1676

719275

2977245timesTo

go-II

1723

1718

3163

2201

1749

8250

15443

8481229

806

1412

816

5967

4700

4067

4911

174

245

298

239

22075

23503

19875

21818

3077245times99HS-18

2016

1970

28542280

3217

13027

3531

6591395

965

285

549

5733

4467

3833

4678

148

228

209

195

2212

721462

1690

020

163

31CV

J-2-5-3-1-3times

1305

1358

1422

2179

1653

1254

2803

1019

44750

178

254

801

411

6033

4467

3367

4622

312

193

330

278

22269

19547

17250

19689

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

1335

1742

2400

1826

922

5226

12806

6318

236

458

1054

583

5633

4433

3600

4556

321

315

259

298

2210

419524

17087

19572

33CV

J-2-5-3-1-3times

96AC

-93

1552

16803260

2164

166710094

11941

7901250

974

1115

779

5533

4533

446748

44

170

202

317

230

2392

123317

19333

2219

0

34CV

J-2-5-3-1-3times

Togo-II

142216072638

1889

1012

4163

11061

5412

261

377

891

510

6167

4967

4533

5222

207

225

325

252

23590

22860

20278

22243

35CV

J-2-5-3-1-3times

99HS-18

1435

1600

2700

1912

3056

3237

7089

4461449

301

658

469

5700

44673800

4656

148

178

225

183

22444

20240

1696

719884

361305times(77371times

BSEC

TCP

-1)

1499

1767

2058

1774

1882

4665

7920

4822

356

426

724

502

566742673800

4578

271

242

305

273

22011

19295

16417

19241

371305times96AC

-93

16781605

2757

2014

1516

10037

11960

7838

311

11381284

911

560040

673400

4356

182

276

251

236

2396

519625

15800

1979

738

1305timesTo

go-II

1177

16802249

1702

902

4676

9388

4989

241

456

795

497

6533

5533

4733

5600

214

266

296

259

21894

20217

19222

20444

391305times99HS-18

1774

1767

2766

2102

2145

4628

10618

5797

301

447

991

580

5933

4500

3733

4722

128

142

180

150

2275

719413

16967

1971

2

40(77371times

BSEC

TCP

-1)times

96AC

-93

1748

1795

31712238

1537

12586

14379

9501

211

1104

1183

833

546744

00346744

44

127

201

226

185

23294

21089

17858

2074

7

10 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(

days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

41(77371times

BSEC

TCP

-1)times

Togo-II

19652007

2653

2208

2136

5601

9503

5747

315

635

1001

650

5533

4833

410048

22

213

241

286

247

22662

22094

17667

20808

42(77371times

BSEC

TCP

-1)times

99HS-18

15011687

2705

1964

1935

7253

11806

6998

314

704

1067

695

5467

4433

5133

5011

177

167

175

173

22305

19277

16392

19325

4396AC

-93timesTo

go-II1759

1618

2123

1834

1539

10347

12212

8033

325

907

1090

774

5900

450039674789

214

269

339

274

23252

21554

16875

20560

4496AC

-93times

99HS-18

1353

1618

2925

1966

3532

7223

11950

7569

440

654

1024

706

5600

4900

4533

5011

149

175

206

177

24494

20698

18111

2110

145

Togo-IItimes

99HS-18

1117

1278

2582

1659

3448

6764

11910

7374

477

767

1207

817

6000

5133

4333

5156

183

203

216

201

17683

15541

14333

15853

Mean

1513

1618

2492

1874

1656

5773

9854

5761

272

538

885

565

586846

534116

4879

218

233

268

240

21297

19547

16529

1912

4SE

101

073

098

mdash079

152

317

mdash014

014

028

mdash351

365

428

mdash036

039

046

mdash985

1173

1211

mdashCD

284

205

276

mdash221

427

890

mdash038

040

079

mdash12

513

015

2mdash

013

014

016

mdash351

418

431

mdash

(c)

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

1642

1667

1744

1684

1504

1349

1526

1460

2783

2405

2634

2607

836

745

908

830

4287

3754

4160

4067

2H7729-2

1693

1817

1950

1820

1012

859

1016

962

3433

2809

2591

2944

649

597

651

632

4445

3668

3607

3906

3G73-107

2000

2028

2077

2035

2280

1966

2312

2186

4695

4023

4229

4316

1281

1170

1463

1305

6975

5989

6541

6502

477245

1523

1630

1728

1627

1796

2489

2702

2329

5423

4851

4498

4924

748

1464

1743

1319

7219

7341

7200

7253

5CV

J-2-5-3-1-3

2282

2692

2787

2587

2411

2643

2901

2652

5857

4836

5024

5239

1206

1335

1769

1437

8268

7479

7925

7891

61305

1851

1951

2054

1952

2026

1763

1908

1899

4657

4414

4463

4511

1468

1336

1539

1448

6683

6177

6371

6410

777371timesBS

ECTCP

-11629

1645

1733

1669

1244

1692

1884

1607

4925

4183

4435

4515

625

1092

1483

1067

6169

5875

6319

6121

896AC

-93

1379

1519

1600

1499

2066

2942

3088

2698

4501

4095

4229

4275

984

1565

1678

1409

6567

7037

7316

6973

9To

go-II

1904

2057

2103

2021

2167

1844

2358

2123

4520

3446

3904

3957

1298

1138

1502

1313

6687

5290

6262

6080

1099HS-18

1475

1485

1633

1531

3180

2904

3382

3155

5633

4139

5205

4992

1656

1536

1879

1690

8813

7043

8587

8147

1H77833-2timesH7729-2

2010

2046

2133

2063

3645

3075

4377

3699

7675

6540

7199

7138

2014

1708

2880

2201

11320

9615

11576

10837

2H77833-2timesG73-107

2066

2101

2122

2096

5002

4053

4601

4552

7882

7430

6963

7425

3146

2565

2808

2840

12884

11482

11564

11977

3H77833-2times77245

1764

2048

2083

1965

3769

4497

4559

4275

8681

8515

8475

8557

2284

3356

3377

3006

12450

13012

13034

12832

4H77833-2times

CVJ-2-5-3-1-3

2205

2383

2733

2441

3693

5655

5220

4856

11656

10287

1010

510683

2198

4488

4176

3621

15348

1594

215325

15538

5H77833-2times1305

2209

2423

2491

2374

6008

5842

6933

6261

15238

14311

13889

14479

3758

4057

5591

4469

21246

2015

320822

2074

0

6H77833-2times(77371times

BSEC

TCP

-1)

1789

1823

1992

1868

3593

4288

4903

4262

8922

6522

7429

7625

2178

3224

3070

2824

12515

10811

12333

11886

7H77833-2times96AC

-93

1739

1960

2011

1903

4557

4350

4818

4575

7916

6068

7290

7092

2411

2529

3191

2710

12474

10419

1210

811667

8H77833-2timesTo

go-II

1927

2389

2406

2241

4185

5828

6215

5409

12319

10279

11023

11207

2433

4254

4631

3773

16504

1610

717238

16616

9H77833-2times99HS-18

1941

2245

2330

2172

4227

4139

4529

4298

9211

8120

7969

8433

3154

3184

3253

3197

13438

12259

12498

1273

210

H7729-2timesG73-107

1968

2109

2158

2079

3725

3042

3488

3418

7313

5956

6174

6481

2822

2001

2357

2393

11038

8998

9662

9899

Advances in Agriculture 11

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

11H7729-2times77245

1847

2048

2200

2032

2467

3759

5466

3898

7672

7449

8921

8014

1356

2350

3796

2500

1013

911208

14387

11911

12H7729-2times

CVJ-2-5-3-1-3

2218

2585

2767

2523

3674

4372

4483

4176

10053

9467

8664

9395

2417

3312

3345

3025

1372

813839

1314

713571

13H7729-2times1305

2169

2338

2547

2352

6728

6166

6792

6562

13333

11662

13301

1276

54128

4501

5660

4763

20061

17828

20093

19327

14H7729-2times(77371times

BSEC

TCP

-1)

1925

2095

2127

2049

3530

4165

4649

4115

9638

6917

7698

8084

1681

2833

3150

2555

1316

8110

82

12347

1219

915

H7729-2times96AC

-93

2012

2070

2180

2087

4955

4447

4596

4666

8738

6882

7762

7794

3754

3558

3181

3498

13693

11329

12359

12460

16H7729-2timesTo

go-II

2138

2428

2563

2377

4949

4660

5118

4909

8549

7243

7191

7661

3586

3353

3028

3322

13498

11903

12310

12570

17H7729-2times99HS-18

1899

1933

2073

1969

4046

4527

4988

4520

8492

7895

8144

8177

2561

3378

3553

3164

12539

12422

1313

212698

18G73-107times77245

1840

2253

2437

2177

2580

4776

5915

4424

11673

10608

10459

10913

1358

3163

4286

2936

14253

15385

16374

15337

19G73-107times

CVJ-2-5-3-1-3

2324

2537

2691

2517

4958

5030

5599

5196

9524

9395

1114

610022

3241

3645

4444

3776

14483

14425

1674

515217

20G73-107times1305

2228

2433

2583

2415

6340

5794

6373

6169

12490

1015

8119

61

11536

4199

4199

5018

4472

18830

1595

218334

1770

6

21G73-107times(77371times

BSEC

TCP

-1)

1943

2128

2220

2097

3936

3980

5770

4562

11697

9858

10303

10619

1874

2369

4181

2808

15633

13839

16073

1518

222

G73-107times96AC

-93

2118

2218

2317

2217

4270

5843

6201

5438

10319

9872

1015

910117

2703

4460

4379

3847

14589

15715

16359

15555

23G73-107timesTo

go-II

2237

2365

2733

2445

5736

5175

6822

5911

12098

10808

1299

611967

3984

3670

5372

4342

17835

1598

219818

17878

24G73-107times99HS-18

2025

2081

2322

2143

3558

4229

6612

4800

12350

9685

11516

1118

42196

2782

5009

3329

1590

813914

1812

915983

2577245timesCV

J-2-5-3-1-3

2109

2360

2675

2381

6760

6558

7299

6872

15225

11861

1416

21374

94225

4752

5839

4939

2198

518418

21461

20621

2677245times1305

1919

2100

2197

2072

5700

5142

5347

5397

11687

10565

10737

10996

4318

3956

4244

4173

17386

1570

816085

16393

2777245times(77371times

BSEC

TCP

-1)

1851

1935

2020

1936

2577

2460

2838

2625

6989

4778

4636

5468

1631

1662

1999

1764

9566

7238

7474

8093

2877245times96AC

-93

1729

1780

2133

1881

5541

5058

5748

5449

11952

8174

9526

9884

3646

3587

4071

3768

17493

13232

15274

15333

2977245timesTo

go-II

2300

2508

2658

2489

3067

5431

5963

4820

11737

11021

1216

411641

1598

4053

4770

3474

14805

16452

1812

616461

3077245times99HS-18

1982

2019

2223

2075

3846

3119

4225

3730

7493

6720

6476

6896

3205

2578

2643

2809

11339

9839

1070

110626

12 Advances in Agriculture

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

31CV

J-2-5-3-1-3times1305

2406

2553

3067

2675

4178

4796

6281

5085

13822

11425

12252

12500

2472

3241

4907

3540

18000

16222

18532

17585

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

2142

2173

2238

2184

4522

5235

5640

5132

11673

9658

10248

10526

2614

3766

4178

3519

1619

514893

15888

15659

33CV

J-2-5-3-1-3times

96AC

-93

2350

2477

2600

2476

4676

4280

6090

5015

11975

9832

10868

10891

3097

2931

4445

3491

16651

14111

1695

815907

34CV

J-2-5-3-1-3times

Togo-II

2093

2666

2989

2583

4058

3727

5190

4325

12588

10717

11217

11507

2742

2570

4185

3166

16645

14443

16407

15832

35CV

J-2-5-3-1-3times

99HS-18

2028

2311

2386

2242

3287

2513

5356

3719

10055

9733

1036

410051

2004

1581

4251

2612

13342

12246

1572

01376

9

361305times(77371times

BSEC

TCP

-1)

2005

2173

2210

2129

3569

4692

5140

4467

11652

9826

10041

10507

2005

3665

4179

3283

15221

14518

1518

214974

371305times96AC

-93

1822

2003

2047

1957

5115

4023

4813

4650

9965

7675

7571

8404

3456

2647

3155

3086

15080

11698

12384

13054

381305timesTo

go-II

2545

2583

2644

2591

6885

7283

6795

6987

12265

11410

12583

12086

4500

6120

5350

5323

1915

018693

19378

19074

391305times99HS-18

2070

2408

2593

2357

5219

4332

4800

4784

1092

97677

8968

9192

3924

3332

3840

3699

1614

912009

1376

913976

40(77371times

BSEC

TCP

-1)

times96AC

-93

2056

2115

2157

2109

3158

4463

4717

4113

9166

7540

8100

8268

1880

3542

3347

2923

12324

12003

12817

12381

41(77371times

BSEC

TCP

-1)

timesTo

go-II

1868

2253

2450

2190

4422

6458

7038

5973

1415

312369

1379

713439

2291

4646

5586

4174

18575

18827

20835

19412

42(77371times

BSEC

TCP

-1)

times99HS-18

1995

2026

2209

2077

2720

2909

3543

3057

7392

4692

5023

5702

1766

2187

2147

2033

10112

7601

8566

8760

4396AC

-93timesTo

go-II

1953

2027

2511

2164

5972

4391

7126

5830

15537

1392

714432

14632

3780

2538

5655

3991

21510

18318

21558

20462

4496AC

-93times99HS-18

1952

2021

2242

2072

3465

2758

4496

3573

5959

5186

6094

5746

2707

2154

2409

2423

9424

7944

10590

9319

45To

go-IItimes

99HS-18

1932

2008

2658

2199

5381

4995

5436

5270

10299

8876

9244

9473

3986

3842

3668

3832

15680

13871

14680

1474

3Mean

1982

2146

2301

2143

3963

4123

4836

4307

9498

8087

8663

8749

2510

2914

3513

2979

13460

12210

13499

13056

SE243

198

245

mdash671

501

626

mdash959

750

822

mdash423

349

344

mdash1155

927

1167

mdashCD

087

071

087

mdash239

178

223

mdash342

267

293

mdash15

112

412

2mdash

411

330

416

mdash

Advances in Agriculture 13Ta

ble4Analysis

ofvaria

ncefor

10times10

half-diallelfor

different

charactersin

threee

nviro

nments

(a)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Germination

()

Rateof

emergence

STI()

SSTI

()

Num

bero

fleavesseedling

Seedlin

gheight

(cm)

Freshweight

(g)seedlin

gDry

weight

(g)seedlin

g

E 1

Replicates

2652731

596768

783393

20700

93018674

33764

1033999

002590

Treatm

ents

541097

0255lowastlowast

8181153lowastlowast

30863092lowastlowast

20680900lowastlowast

1100708lowastlowast

2106317lowastlowast

930829527lowastlowast

319767lowastlowast

Parents

95118

163lowastlowast

14289925lowastlowast

59114816lowastlowast

18880254lowastlowast

1084227lowastlowast

1087906lowastlowast

22561719lowastlowast

042162lowastlowast

Hybrid

s44

11423573lowastlowast

3398933lowastlowast

25697199lowastlowast

20633716lowastlowast

472874lowastlowast

1391526lowastlowast

674123518lowastlowast

261839lowastlowast

Parentsv

ersush

ybrid

s1

43693104lowastlowast

163619

916lowastlowast

3896838

3896

2793lowastlowast

28873741lowastlowast

4272

2798lowastlowast

234144289lowastlowast

5367022lowastlowast

Error

108

281184

1356832

1173684

674337

040104

306

462

2015

1619

005535

E 2

Replicates

2583819

3707389lowast

1596635

198738

034925

448213

899529

007177

Treatm

ents

549997443lowastlowast

2825177lowastlowast

35378177lowastlowast

19074306lowastlowast

665284lowastlowast

1764

081lowastlowast

257694570lowastlowast

2177519lowastlowast

Parents

96507784lowastlowast

3718

879lowastlowast

69866289lowastlowast

1798

5404lowastlowast

705379lowastlowast

1408518lowastlowast

10612519lowastlowast

077429lowastlowast

Hybrid

s44

10569731lowastlowast

2127302lowastlowast

2910

2537lowastlowast

19429240lowastlowast

329256lowastlowast

7000

03lowastlowast

186026552lowastlowast

1483428lowastlowast

Parentsv

ersush

ybrid

s1

16223710lowastlowast

25488361lowastlowast

1113

326

13257362lowastlowast

15089673lowastlowast

5178

3569lowastlowast

5634825791lowastlowast

51618348lowastlowast

Error

108

334760

1097039

1049505

635570

06764

116

0576

694613

006206

E 3

Replicates

2894398

4808815lowast

mdashmdash

051076

095989

3768421

030220

Treatm

ents

541261560

0lowastlowast

4842216lowastlowast

mdashmdash

2811077lowastlowast

5947316lowastlowast

3870844

50lowastlowast

3445076lowastlowast

Parents

97135

735lowastlowast

1097

6388lowastlowast

mdashmdash

1468699lowastlowast

1112

764lowastlowast

136081650lowastlowast

1307836lowastlowast

Hybrid

s44

13217740lowastlowast

1379360

mdashmdash

15540

71lowastlowast

4581632lowastlowast

252084767lowastlowast

2378032lowastlowast

Parentsv

ersush

ybrid

s1

35440

180lowastlowast

102000335lowastlowast

mdashmdash

70200767lowastlowast

109548362lowastlowast

8586095705lowastlowast

69630188lowastlowast

Error

108

782907

1085444

mdashmdash

139771

291325

3019

169

02400

4(b)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Panicle

emergence

Effectiv

etillersplant

Planth

eight

Earlength

Earw

eightp

lant

Dry

fodd

eryieldplant

Grain

yieldplant

Totalbiological

yieldplant

E 1

Replicates

231860

61lowastlowast

416348lowastlowast

1672

9472lowast

1706

411077192

9634539

392777

115544

59Treatm

ents

544152

368lowastlowast

085657lowastlowast

326706089lowastlowast

1602572lowastlowast

6177

0406lowastlowast

304113

204lowastlowast

34285030lowastlowast

575617

582lowastlowast

Parents

94511481lowastlowast

018538lowastlowast

140807476lowastlowast

2240093lowastlowast

11653

959lowastlowast

27014155lowastlowast

3858650lowastlowast

61321919lowastlowast

Hybrid

s44

3201347lowastlowast

100797lowastlowast

110395657lowastlowast

1010

497lowastlowast

40296671lowastlowast

171271411lowastlowast

2413

6880lowastlowast

302999650lowastlowast

Parentsv

ersush

ybrid

s1

4276

5286lowastlowast

02360

9lowast11517452

616lowastlowast

2191

6167lowastlowast

1457662749lowastlowast

8643043524lowastlowast

7546

41053lowastlowast

17199467546lowastlowast

Error

108

468159

004907

3692099

224610

17114

303498126

682350

5075268

E 2

Replicates

2656364

002556

61575565lowastlowast

828842lowastlowast

589094

2268506

260877

1507116

Treatm

ents

542999618lowastlowast

069822lowastlowast

268998723lowastlowast

2436886lowastlowast

60592784lowastlowast

247259877lowastlowast

4192

0073lowastlowast

513483489lowastlowast

Parents

926300

00lowastlowast

048079lowastlowast

165555

405lowastlowast

3871874lowastlowast

1412

2393lowastlowast

19543682lowastlowast

3132

426lowastlowast

57230706lowastlowast

Hybrid

s44

2533

165lowastlowast

07344

9lowastlowast

9616

2857lowastlowast

1463359lowastlowast

35495366lowastlowast

154759288lowastlowast

26262465lowastlowast

293492304lowastlowast

Parentsv

ersush

ybrid

s1

26850135lowastlowast

105929lowastlowast

880476

6697lowastlowast

32357161lowastlowast

1583112746lowastlowast

6366731560lowastlowast

107994

3646lowastlowast

14299370699lowastlowast

Error

108

507598

005829

5234122

149406

955133

2142411

462311

3273886

E 3

Replicates

2029697

021161

9459847

143746

985141

46800

6492

6334

3345160

Treatm

ents

548045499lowastlowast

094511lowastlowast

192420108lowastlowast

3279225lowastlowast

72049211lowastlowast

298953

021lowastlowast

55061308lowastlowast

651732994lowastlowast

Parents

98010

741lowastlowast

094166lowastlowast

116037343lowastlowast

3702185lowastlowast

16305190lowastlowast

2323644

0lowastlowast

4553

893lowastlowast

71484982lowastlowast

Hybrid

s44

6254377lowastlowast

095514lowastlowast

7378

1513lowastlowast

2188348lowastlowast

31813193lowastlowast

190263916lowastlowast

31572179lowastlowast

368689934lowastlowast

Parentsv

ersush

ybrid

s1

8716

7710lowastlowast

053496lowast

609996

3199lowastlowast

47471212lowastlowast

2344

130203lowastlowast

756272

2861lowastlowast

154314

9707lowastlowast

18327859

719lowastlowast

Error

108

696981

007883

5578505

228608

1491014

2574

223

449588

5187705

lowast

Sign

ificant

at119875=005

lowastlowastSign

ificant

at119875=001

14 Advances in Agriculture

Table 5 Mean performance of the genotypes for membrane thermostability in two environments

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594

Advances in Agriculture 15

Table 5 Continued

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434

Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash

lowastValues are transformed values

0

6

12

18

24

30

36

42

48

54

minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity

G73-10796AC-93CVJ-2-5-3-1-3

Togo-II99HS-18

H77833-2H7729-2772451305

H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305

1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305

H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305

77245 times 1305

77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305

77371 times BSECT CP-1

H77833-2 times G73-107

Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits

moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling

survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The

16 Advances in Agriculture

Table6Po

oled

correla

tioncoeffi

cientamon

gvario

uscharactersin

threee

nviro

nments

Characters

Germination

Rateof

emergence

STI

SSTI

Leaves

seedlin

gSeedlin

gheight

Fresh

weight

Dry

weight

MTS

Panicle

emergence

Effectiv

etillersplant

Plant

height

Earlength

Ear

weightp

lant

Dry

fodd

eryield

plant

Grain

yield

plant

Total

biological

yield

plant

Germination

1000

0357lowastlowast

0353lowastlowast

0590lowastlowast

0230

0373lowastlowast

0293lowast

0260

0276lowastminus0135minus0478lowastlowast

0326lowast

minus0069minus0104

minus0190

minus0145

minus0167

Rateof

emergence

1000

0233

0351lowastlowast

0605lowastlowast

0585lowastlowast

0551lowastlowast

0524lowastlowast

0139minus0593lowastlowast

0032

0517lowastlowast

0217

0388lowastlowast

0361lowastlowast

0348lowastlowast

0377lowastlowast

STI

1000

0837lowastlowast

0241

0129

0081

004

80890lowastlowast

minus0215

minus0211

0030minus0007minus0043

minus0101

minus0111

minus0084

SSTI

1000

0233

0264

0183

0140

0745lowastlowast

minus0247minus0268lowast

0184minus0017minus006

6minus0129

minus0144

minus0112

Num

bero

fleaves

1000

0683lowastlowast

0633lowastlowast

060

4lowastlowast

0176minus0474lowastlowast

0109

0576lowastlowast

0496lowastlowast

064

1lowastlowast

0607lowastlowast

0630lowastlowast

0630lowastlowast

Seedlin

gheight

1000

0703lowastlowast

0663lowastlowast

0031minus0493lowastlowast

minus0115

0735lowastlowast

0360lowastlowast

0526lowastlowast

0520lowastlowast

0515lowastlowast

0532lowastlowast

Freshweight

1000

0957lowastlowast

minus0016

0425lowastlowast

minus0082

0661lowastlowast

0351lowastlowast

0581lowastlowast

0496lowastlowast

0576lowastlowast

0533lowastlowast

Dry

weight

1000minus0033minus0428lowastlowast

minus0016

0634lowastlowast

0354lowastlowast

0620lowastlowast

0509lowastlowast

0614lowastlowast

0554lowastlowast

MTS

1000minus040

minus0177minus0037

0015

minus0074

minus0138

minus0131

minus0120

Panicle

emergence

1000

minus0150minus0269lowast

0022minus0329lowast

minus0337lowast

minus0280lowast

minus0341lowast

Effectiv

etillersplant

1000

minus0012

0170

0366lowastlowast

040

5lowastlowast

0323lowast

0401lowastlowast

Planth

eight

1000

0650lowastlowast

0603lowastlowast

0596lowastlowast

0619lowastlowast

0610lowastlowast

Earlength

1000

0608lowastlowast

0671lowastlowast

064

8lowastlowast

066

4lowastlowast

Earw

eightp

lant

1000

0910lowastlowast

0984lowastlowast

0957lowastlowast

Dry

fodd

eryield

plant

1000

0907lowastlowast

0991lowastlowast

Grain

yield

plant

1000

0950lowastlowast

Totalbiological

yield

plant

1000

lowastSign

ificant

at119875=005lowastlowastSign

ificant

at119875=001

Advances in Agriculture 17

faster germination and early vigour in seedling could act asphysical indicators for heat tolerance

Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits

Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics

Conflict of Interests

The authors declare that there is no conflict of interestsregarding the publication of this paper

References

[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981

[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993

[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006

[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993

[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013

[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007

[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965

[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011

[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001

[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009

[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962

[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967

[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963

[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001

Submit your manuscripts athttpwwwhindawicom

Nutrition and Metabolism

Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Food ScienceInternational Journal of

Agronomy

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Microbiology

The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom

Applied ampEnvironmentalSoil Science

Volume 2014

AgricultureAdvances in

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

BiodiversityInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

GenomicsInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Plant GenomicsInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Biotechnology Research International

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Forestry ResearchInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

EcologyInternational Journal of

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Page 10: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

10 Advances in Agriculture

(b)Con

tinued

Sr

NoGenotypes

Seedlin

gheight

(cm)

Freshweight(g)seedling

Dry

weight(g)seedling

Panicle

emergence(

days)

Effectiv

etillersplant

Planth

eight(cm

)E 1

E 2E 3

Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

41(77371times

BSEC

TCP

-1)times

Togo-II

19652007

2653

2208

2136

5601

9503

5747

315

635

1001

650

5533

4833

410048

22

213

241

286

247

22662

22094

17667

20808

42(77371times

BSEC

TCP

-1)times

99HS-18

15011687

2705

1964

1935

7253

11806

6998

314

704

1067

695

5467

4433

5133

5011

177

167

175

173

22305

19277

16392

19325

4396AC

-93timesTo

go-II1759

1618

2123

1834

1539

10347

12212

8033

325

907

1090

774

5900

450039674789

214

269

339

274

23252

21554

16875

20560

4496AC

-93times

99HS-18

1353

1618

2925

1966

3532

7223

11950

7569

440

654

1024

706

5600

4900

4533

5011

149

175

206

177

24494

20698

18111

2110

145

Togo-IItimes

99HS-18

1117

1278

2582

1659

3448

6764

11910

7374

477

767

1207

817

6000

5133

4333

5156

183

203

216

201

17683

15541

14333

15853

Mean

1513

1618

2492

1874

1656

5773

9854

5761

272

538

885

565

586846

534116

4879

218

233

268

240

21297

19547

16529

1912

4SE

101

073

098

mdash079

152

317

mdash014

014

028

mdash351

365

428

mdash036

039

046

mdash985

1173

1211

mdashCD

284

205

276

mdash221

427

890

mdash038

040

079

mdash12

513

015

2mdash

013

014

016

mdash351

418

431

mdash

(c)

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

1H77833-2

1642

1667

1744

1684

1504

1349

1526

1460

2783

2405

2634

2607

836

745

908

830

4287

3754

4160

4067

2H7729-2

1693

1817

1950

1820

1012

859

1016

962

3433

2809

2591

2944

649

597

651

632

4445

3668

3607

3906

3G73-107

2000

2028

2077

2035

2280

1966

2312

2186

4695

4023

4229

4316

1281

1170

1463

1305

6975

5989

6541

6502

477245

1523

1630

1728

1627

1796

2489

2702

2329

5423

4851

4498

4924

748

1464

1743

1319

7219

7341

7200

7253

5CV

J-2-5-3-1-3

2282

2692

2787

2587

2411

2643

2901

2652

5857

4836

5024

5239

1206

1335

1769

1437

8268

7479

7925

7891

61305

1851

1951

2054

1952

2026

1763

1908

1899

4657

4414

4463

4511

1468

1336

1539

1448

6683

6177

6371

6410

777371timesBS

ECTCP

-11629

1645

1733

1669

1244

1692

1884

1607

4925

4183

4435

4515

625

1092

1483

1067

6169

5875

6319

6121

896AC

-93

1379

1519

1600

1499

2066

2942

3088

2698

4501

4095

4229

4275

984

1565

1678

1409

6567

7037

7316

6973

9To

go-II

1904

2057

2103

2021

2167

1844

2358

2123

4520

3446

3904

3957

1298

1138

1502

1313

6687

5290

6262

6080

1099HS-18

1475

1485

1633

1531

3180

2904

3382

3155

5633

4139

5205

4992

1656

1536

1879

1690

8813

7043

8587

8147

1H77833-2timesH7729-2

2010

2046

2133

2063

3645

3075

4377

3699

7675

6540

7199

7138

2014

1708

2880

2201

11320

9615

11576

10837

2H77833-2timesG73-107

2066

2101

2122

2096

5002

4053

4601

4552

7882

7430

6963

7425

3146

2565

2808

2840

12884

11482

11564

11977

3H77833-2times77245

1764

2048

2083

1965

3769

4497

4559

4275

8681

8515

8475

8557

2284

3356

3377

3006

12450

13012

13034

12832

4H77833-2times

CVJ-2-5-3-1-3

2205

2383

2733

2441

3693

5655

5220

4856

11656

10287

1010

510683

2198

4488

4176

3621

15348

1594

215325

15538

5H77833-2times1305

2209

2423

2491

2374

6008

5842

6933

6261

15238

14311

13889

14479

3758

4057

5591

4469

21246

2015

320822

2074

0

6H77833-2times(77371times

BSEC

TCP

-1)

1789

1823

1992

1868

3593

4288

4903

4262

8922

6522

7429

7625

2178

3224

3070

2824

12515

10811

12333

11886

7H77833-2times96AC

-93

1739

1960

2011

1903

4557

4350

4818

4575

7916

6068

7290

7092

2411

2529

3191

2710

12474

10419

1210

811667

8H77833-2timesTo

go-II

1927

2389

2406

2241

4185

5828

6215

5409

12319

10279

11023

11207

2433

4254

4631

3773

16504

1610

717238

16616

9H77833-2times99HS-18

1941

2245

2330

2172

4227

4139

4529

4298

9211

8120

7969

8433

3154

3184

3253

3197

13438

12259

12498

1273

210

H7729-2timesG73-107

1968

2109

2158

2079

3725

3042

3488

3418

7313

5956

6174

6481

2822

2001

2357

2393

11038

8998

9662

9899

Advances in Agriculture 11

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

11H7729-2times77245

1847

2048

2200

2032

2467

3759

5466

3898

7672

7449

8921

8014

1356

2350

3796

2500

1013

911208

14387

11911

12H7729-2times

CVJ-2-5-3-1-3

2218

2585

2767

2523

3674

4372

4483

4176

10053

9467

8664

9395

2417

3312

3345

3025

1372

813839

1314

713571

13H7729-2times1305

2169

2338

2547

2352

6728

6166

6792

6562

13333

11662

13301

1276

54128

4501

5660

4763

20061

17828

20093

19327

14H7729-2times(77371times

BSEC

TCP

-1)

1925

2095

2127

2049

3530

4165

4649

4115

9638

6917

7698

8084

1681

2833

3150

2555

1316

8110

82

12347

1219

915

H7729-2times96AC

-93

2012

2070

2180

2087

4955

4447

4596

4666

8738

6882

7762

7794

3754

3558

3181

3498

13693

11329

12359

12460

16H7729-2timesTo

go-II

2138

2428

2563

2377

4949

4660

5118

4909

8549

7243

7191

7661

3586

3353

3028

3322

13498

11903

12310

12570

17H7729-2times99HS-18

1899

1933

2073

1969

4046

4527

4988

4520

8492

7895

8144

8177

2561

3378

3553

3164

12539

12422

1313

212698

18G73-107times77245

1840

2253

2437

2177

2580

4776

5915

4424

11673

10608

10459

10913

1358

3163

4286

2936

14253

15385

16374

15337

19G73-107times

CVJ-2-5-3-1-3

2324

2537

2691

2517

4958

5030

5599

5196

9524

9395

1114

610022

3241

3645

4444

3776

14483

14425

1674

515217

20G73-107times1305

2228

2433

2583

2415

6340

5794

6373

6169

12490

1015

8119

61

11536

4199

4199

5018

4472

18830

1595

218334

1770

6

21G73-107times(77371times

BSEC

TCP

-1)

1943

2128

2220

2097

3936

3980

5770

4562

11697

9858

10303

10619

1874

2369

4181

2808

15633

13839

16073

1518

222

G73-107times96AC

-93

2118

2218

2317

2217

4270

5843

6201

5438

10319

9872

1015

910117

2703

4460

4379

3847

14589

15715

16359

15555

23G73-107timesTo

go-II

2237

2365

2733

2445

5736

5175

6822

5911

12098

10808

1299

611967

3984

3670

5372

4342

17835

1598

219818

17878

24G73-107times99HS-18

2025

2081

2322

2143

3558

4229

6612

4800

12350

9685

11516

1118

42196

2782

5009

3329

1590

813914

1812

915983

2577245timesCV

J-2-5-3-1-3

2109

2360

2675

2381

6760

6558

7299

6872

15225

11861

1416

21374

94225

4752

5839

4939

2198

518418

21461

20621

2677245times1305

1919

2100

2197

2072

5700

5142

5347

5397

11687

10565

10737

10996

4318

3956

4244

4173

17386

1570

816085

16393

2777245times(77371times

BSEC

TCP

-1)

1851

1935

2020

1936

2577

2460

2838

2625

6989

4778

4636

5468

1631

1662

1999

1764

9566

7238

7474

8093

2877245times96AC

-93

1729

1780

2133

1881

5541

5058

5748

5449

11952

8174

9526

9884

3646

3587

4071

3768

17493

13232

15274

15333

2977245timesTo

go-II

2300

2508

2658

2489

3067

5431

5963

4820

11737

11021

1216

411641

1598

4053

4770

3474

14805

16452

1812

616461

3077245times99HS-18

1982

2019

2223

2075

3846

3119

4225

3730

7493

6720

6476

6896

3205

2578

2643

2809

11339

9839

1070

110626

12 Advances in Agriculture

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

31CV

J-2-5-3-1-3times1305

2406

2553

3067

2675

4178

4796

6281

5085

13822

11425

12252

12500

2472

3241

4907

3540

18000

16222

18532

17585

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

2142

2173

2238

2184

4522

5235

5640

5132

11673

9658

10248

10526

2614

3766

4178

3519

1619

514893

15888

15659

33CV

J-2-5-3-1-3times

96AC

-93

2350

2477

2600

2476

4676

4280

6090

5015

11975

9832

10868

10891

3097

2931

4445

3491

16651

14111

1695

815907

34CV

J-2-5-3-1-3times

Togo-II

2093

2666

2989

2583

4058

3727

5190

4325

12588

10717

11217

11507

2742

2570

4185

3166

16645

14443

16407

15832

35CV

J-2-5-3-1-3times

99HS-18

2028

2311

2386

2242

3287

2513

5356

3719

10055

9733

1036

410051

2004

1581

4251

2612

13342

12246

1572

01376

9

361305times(77371times

BSEC

TCP

-1)

2005

2173

2210

2129

3569

4692

5140

4467

11652

9826

10041

10507

2005

3665

4179

3283

15221

14518

1518

214974

371305times96AC

-93

1822

2003

2047

1957

5115

4023

4813

4650

9965

7675

7571

8404

3456

2647

3155

3086

15080

11698

12384

13054

381305timesTo

go-II

2545

2583

2644

2591

6885

7283

6795

6987

12265

11410

12583

12086

4500

6120

5350

5323

1915

018693

19378

19074

391305times99HS-18

2070

2408

2593

2357

5219

4332

4800

4784

1092

97677

8968

9192

3924

3332

3840

3699

1614

912009

1376

913976

40(77371times

BSEC

TCP

-1)

times96AC

-93

2056

2115

2157

2109

3158

4463

4717

4113

9166

7540

8100

8268

1880

3542

3347

2923

12324

12003

12817

12381

41(77371times

BSEC

TCP

-1)

timesTo

go-II

1868

2253

2450

2190

4422

6458

7038

5973

1415

312369

1379

713439

2291

4646

5586

4174

18575

18827

20835

19412

42(77371times

BSEC

TCP

-1)

times99HS-18

1995

2026

2209

2077

2720

2909

3543

3057

7392

4692

5023

5702

1766

2187

2147

2033

10112

7601

8566

8760

4396AC

-93timesTo

go-II

1953

2027

2511

2164

5972

4391

7126

5830

15537

1392

714432

14632

3780

2538

5655

3991

21510

18318

21558

20462

4496AC

-93times99HS-18

1952

2021

2242

2072

3465

2758

4496

3573

5959

5186

6094

5746

2707

2154

2409

2423

9424

7944

10590

9319

45To

go-IItimes

99HS-18

1932

2008

2658

2199

5381

4995

5436

5270

10299

8876

9244

9473

3986

3842

3668

3832

15680

13871

14680

1474

3Mean

1982

2146

2301

2143

3963

4123

4836

4307

9498

8087

8663

8749

2510

2914

3513

2979

13460

12210

13499

13056

SE243

198

245

mdash671

501

626

mdash959

750

822

mdash423

349

344

mdash1155

927

1167

mdashCD

087

071

087

mdash239

178

223

mdash342

267

293

mdash15

112

412

2mdash

411

330

416

mdash

Advances in Agriculture 13Ta

ble4Analysis

ofvaria

ncefor

10times10

half-diallelfor

different

charactersin

threee

nviro

nments

(a)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Germination

()

Rateof

emergence

STI()

SSTI

()

Num

bero

fleavesseedling

Seedlin

gheight

(cm)

Freshweight

(g)seedlin

gDry

weight

(g)seedlin

g

E 1

Replicates

2652731

596768

783393

20700

93018674

33764

1033999

002590

Treatm

ents

541097

0255lowastlowast

8181153lowastlowast

30863092lowastlowast

20680900lowastlowast

1100708lowastlowast

2106317lowastlowast

930829527lowastlowast

319767lowastlowast

Parents

95118

163lowastlowast

14289925lowastlowast

59114816lowastlowast

18880254lowastlowast

1084227lowastlowast

1087906lowastlowast

22561719lowastlowast

042162lowastlowast

Hybrid

s44

11423573lowastlowast

3398933lowastlowast

25697199lowastlowast

20633716lowastlowast

472874lowastlowast

1391526lowastlowast

674123518lowastlowast

261839lowastlowast

Parentsv

ersush

ybrid

s1

43693104lowastlowast

163619

916lowastlowast

3896838

3896

2793lowastlowast

28873741lowastlowast

4272

2798lowastlowast

234144289lowastlowast

5367022lowastlowast

Error

108

281184

1356832

1173684

674337

040104

306

462

2015

1619

005535

E 2

Replicates

2583819

3707389lowast

1596635

198738

034925

448213

899529

007177

Treatm

ents

549997443lowastlowast

2825177lowastlowast

35378177lowastlowast

19074306lowastlowast

665284lowastlowast

1764

081lowastlowast

257694570lowastlowast

2177519lowastlowast

Parents

96507784lowastlowast

3718

879lowastlowast

69866289lowastlowast

1798

5404lowastlowast

705379lowastlowast

1408518lowastlowast

10612519lowastlowast

077429lowastlowast

Hybrid

s44

10569731lowastlowast

2127302lowastlowast

2910

2537lowastlowast

19429240lowastlowast

329256lowastlowast

7000

03lowastlowast

186026552lowastlowast

1483428lowastlowast

Parentsv

ersush

ybrid

s1

16223710lowastlowast

25488361lowastlowast

1113

326

13257362lowastlowast

15089673lowastlowast

5178

3569lowastlowast

5634825791lowastlowast

51618348lowastlowast

Error

108

334760

1097039

1049505

635570

06764

116

0576

694613

006206

E 3

Replicates

2894398

4808815lowast

mdashmdash

051076

095989

3768421

030220

Treatm

ents

541261560

0lowastlowast

4842216lowastlowast

mdashmdash

2811077lowastlowast

5947316lowastlowast

3870844

50lowastlowast

3445076lowastlowast

Parents

97135

735lowastlowast

1097

6388lowastlowast

mdashmdash

1468699lowastlowast

1112

764lowastlowast

136081650lowastlowast

1307836lowastlowast

Hybrid

s44

13217740lowastlowast

1379360

mdashmdash

15540

71lowastlowast

4581632lowastlowast

252084767lowastlowast

2378032lowastlowast

Parentsv

ersush

ybrid

s1

35440

180lowastlowast

102000335lowastlowast

mdashmdash

70200767lowastlowast

109548362lowastlowast

8586095705lowastlowast

69630188lowastlowast

Error

108

782907

1085444

mdashmdash

139771

291325

3019

169

02400

4(b)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Panicle

emergence

Effectiv

etillersplant

Planth

eight

Earlength

Earw

eightp

lant

Dry

fodd

eryieldplant

Grain

yieldplant

Totalbiological

yieldplant

E 1

Replicates

231860

61lowastlowast

416348lowastlowast

1672

9472lowast

1706

411077192

9634539

392777

115544

59Treatm

ents

544152

368lowastlowast

085657lowastlowast

326706089lowastlowast

1602572lowastlowast

6177

0406lowastlowast

304113

204lowastlowast

34285030lowastlowast

575617

582lowastlowast

Parents

94511481lowastlowast

018538lowastlowast

140807476lowastlowast

2240093lowastlowast

11653

959lowastlowast

27014155lowastlowast

3858650lowastlowast

61321919lowastlowast

Hybrid

s44

3201347lowastlowast

100797lowastlowast

110395657lowastlowast

1010

497lowastlowast

40296671lowastlowast

171271411lowastlowast

2413

6880lowastlowast

302999650lowastlowast

Parentsv

ersush

ybrid

s1

4276

5286lowastlowast

02360

9lowast11517452

616lowastlowast

2191

6167lowastlowast

1457662749lowastlowast

8643043524lowastlowast

7546

41053lowastlowast

17199467546lowastlowast

Error

108

468159

004907

3692099

224610

17114

303498126

682350

5075268

E 2

Replicates

2656364

002556

61575565lowastlowast

828842lowastlowast

589094

2268506

260877

1507116

Treatm

ents

542999618lowastlowast

069822lowastlowast

268998723lowastlowast

2436886lowastlowast

60592784lowastlowast

247259877lowastlowast

4192

0073lowastlowast

513483489lowastlowast

Parents

926300

00lowastlowast

048079lowastlowast

165555

405lowastlowast

3871874lowastlowast

1412

2393lowastlowast

19543682lowastlowast

3132

426lowastlowast

57230706lowastlowast

Hybrid

s44

2533

165lowastlowast

07344

9lowastlowast

9616

2857lowastlowast

1463359lowastlowast

35495366lowastlowast

154759288lowastlowast

26262465lowastlowast

293492304lowastlowast

Parentsv

ersush

ybrid

s1

26850135lowastlowast

105929lowastlowast

880476

6697lowastlowast

32357161lowastlowast

1583112746lowastlowast

6366731560lowastlowast

107994

3646lowastlowast

14299370699lowastlowast

Error

108

507598

005829

5234122

149406

955133

2142411

462311

3273886

E 3

Replicates

2029697

021161

9459847

143746

985141

46800

6492

6334

3345160

Treatm

ents

548045499lowastlowast

094511lowastlowast

192420108lowastlowast

3279225lowastlowast

72049211lowastlowast

298953

021lowastlowast

55061308lowastlowast

651732994lowastlowast

Parents

98010

741lowastlowast

094166lowastlowast

116037343lowastlowast

3702185lowastlowast

16305190lowastlowast

2323644

0lowastlowast

4553

893lowastlowast

71484982lowastlowast

Hybrid

s44

6254377lowastlowast

095514lowastlowast

7378

1513lowastlowast

2188348lowastlowast

31813193lowastlowast

190263916lowastlowast

31572179lowastlowast

368689934lowastlowast

Parentsv

ersush

ybrid

s1

8716

7710lowastlowast

053496lowast

609996

3199lowastlowast

47471212lowastlowast

2344

130203lowastlowast

756272

2861lowastlowast

154314

9707lowastlowast

18327859

719lowastlowast

Error

108

696981

007883

5578505

228608

1491014

2574

223

449588

5187705

lowast

Sign

ificant

at119875=005

lowastlowastSign

ificant

at119875=001

14 Advances in Agriculture

Table 5 Mean performance of the genotypes for membrane thermostability in two environments

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594

Advances in Agriculture 15

Table 5 Continued

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434

Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash

lowastValues are transformed values

0

6

12

18

24

30

36

42

48

54

minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity

G73-10796AC-93CVJ-2-5-3-1-3

Togo-II99HS-18

H77833-2H7729-2772451305

H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305

1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305

H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305

77245 times 1305

77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305

77371 times BSECT CP-1

H77833-2 times G73-107

Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits

moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling

survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The

16 Advances in Agriculture

Table6Po

oled

correla

tioncoeffi

cientamon

gvario

uscharactersin

threee

nviro

nments

Characters

Germination

Rateof

emergence

STI

SSTI

Leaves

seedlin

gSeedlin

gheight

Fresh

weight

Dry

weight

MTS

Panicle

emergence

Effectiv

etillersplant

Plant

height

Earlength

Ear

weightp

lant

Dry

fodd

eryield

plant

Grain

yield

plant

Total

biological

yield

plant

Germination

1000

0357lowastlowast

0353lowastlowast

0590lowastlowast

0230

0373lowastlowast

0293lowast

0260

0276lowastminus0135minus0478lowastlowast

0326lowast

minus0069minus0104

minus0190

minus0145

minus0167

Rateof

emergence

1000

0233

0351lowastlowast

0605lowastlowast

0585lowastlowast

0551lowastlowast

0524lowastlowast

0139minus0593lowastlowast

0032

0517lowastlowast

0217

0388lowastlowast

0361lowastlowast

0348lowastlowast

0377lowastlowast

STI

1000

0837lowastlowast

0241

0129

0081

004

80890lowastlowast

minus0215

minus0211

0030minus0007minus0043

minus0101

minus0111

minus0084

SSTI

1000

0233

0264

0183

0140

0745lowastlowast

minus0247minus0268lowast

0184minus0017minus006

6minus0129

minus0144

minus0112

Num

bero

fleaves

1000

0683lowastlowast

0633lowastlowast

060

4lowastlowast

0176minus0474lowastlowast

0109

0576lowastlowast

0496lowastlowast

064

1lowastlowast

0607lowastlowast

0630lowastlowast

0630lowastlowast

Seedlin

gheight

1000

0703lowastlowast

0663lowastlowast

0031minus0493lowastlowast

minus0115

0735lowastlowast

0360lowastlowast

0526lowastlowast

0520lowastlowast

0515lowastlowast

0532lowastlowast

Freshweight

1000

0957lowastlowast

minus0016

0425lowastlowast

minus0082

0661lowastlowast

0351lowastlowast

0581lowastlowast

0496lowastlowast

0576lowastlowast

0533lowastlowast

Dry

weight

1000minus0033minus0428lowastlowast

minus0016

0634lowastlowast

0354lowastlowast

0620lowastlowast

0509lowastlowast

0614lowastlowast

0554lowastlowast

MTS

1000minus040

minus0177minus0037

0015

minus0074

minus0138

minus0131

minus0120

Panicle

emergence

1000

minus0150minus0269lowast

0022minus0329lowast

minus0337lowast

minus0280lowast

minus0341lowast

Effectiv

etillersplant

1000

minus0012

0170

0366lowastlowast

040

5lowastlowast

0323lowast

0401lowastlowast

Planth

eight

1000

0650lowastlowast

0603lowastlowast

0596lowastlowast

0619lowastlowast

0610lowastlowast

Earlength

1000

0608lowastlowast

0671lowastlowast

064

8lowastlowast

066

4lowastlowast

Earw

eightp

lant

1000

0910lowastlowast

0984lowastlowast

0957lowastlowast

Dry

fodd

eryield

plant

1000

0907lowastlowast

0991lowastlowast

Grain

yield

plant

1000

0950lowastlowast

Totalbiological

yield

plant

1000

lowastSign

ificant

at119875=005lowastlowastSign

ificant

at119875=001

Advances in Agriculture 17

faster germination and early vigour in seedling could act asphysical indicators for heat tolerance

Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits

Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics

Conflict of Interests

The authors declare that there is no conflict of interestsregarding the publication of this paper

References

[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981

[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993

[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006

[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993

[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013

[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007

[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965

[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011

[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001

[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009

[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962

[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967

[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963

[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001

Submit your manuscripts athttpwwwhindawicom

Nutrition and Metabolism

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Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

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AgricultureAdvances in

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Page 11: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

Advances in Agriculture 11

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

11H7729-2times77245

1847

2048

2200

2032

2467

3759

5466

3898

7672

7449

8921

8014

1356

2350

3796

2500

1013

911208

14387

11911

12H7729-2times

CVJ-2-5-3-1-3

2218

2585

2767

2523

3674

4372

4483

4176

10053

9467

8664

9395

2417

3312

3345

3025

1372

813839

1314

713571

13H7729-2times1305

2169

2338

2547

2352

6728

6166

6792

6562

13333

11662

13301

1276

54128

4501

5660

4763

20061

17828

20093

19327

14H7729-2times(77371times

BSEC

TCP

-1)

1925

2095

2127

2049

3530

4165

4649

4115

9638

6917

7698

8084

1681

2833

3150

2555

1316

8110

82

12347

1219

915

H7729-2times96AC

-93

2012

2070

2180

2087

4955

4447

4596

4666

8738

6882

7762

7794

3754

3558

3181

3498

13693

11329

12359

12460

16H7729-2timesTo

go-II

2138

2428

2563

2377

4949

4660

5118

4909

8549

7243

7191

7661

3586

3353

3028

3322

13498

11903

12310

12570

17H7729-2times99HS-18

1899

1933

2073

1969

4046

4527

4988

4520

8492

7895

8144

8177

2561

3378

3553

3164

12539

12422

1313

212698

18G73-107times77245

1840

2253

2437

2177

2580

4776

5915

4424

11673

10608

10459

10913

1358

3163

4286

2936

14253

15385

16374

15337

19G73-107times

CVJ-2-5-3-1-3

2324

2537

2691

2517

4958

5030

5599

5196

9524

9395

1114

610022

3241

3645

4444

3776

14483

14425

1674

515217

20G73-107times1305

2228

2433

2583

2415

6340

5794

6373

6169

12490

1015

8119

61

11536

4199

4199

5018

4472

18830

1595

218334

1770

6

21G73-107times(77371times

BSEC

TCP

-1)

1943

2128

2220

2097

3936

3980

5770

4562

11697

9858

10303

10619

1874

2369

4181

2808

15633

13839

16073

1518

222

G73-107times96AC

-93

2118

2218

2317

2217

4270

5843

6201

5438

10319

9872

1015

910117

2703

4460

4379

3847

14589

15715

16359

15555

23G73-107timesTo

go-II

2237

2365

2733

2445

5736

5175

6822

5911

12098

10808

1299

611967

3984

3670

5372

4342

17835

1598

219818

17878

24G73-107times99HS-18

2025

2081

2322

2143

3558

4229

6612

4800

12350

9685

11516

1118

42196

2782

5009

3329

1590

813914

1812

915983

2577245timesCV

J-2-5-3-1-3

2109

2360

2675

2381

6760

6558

7299

6872

15225

11861

1416

21374

94225

4752

5839

4939

2198

518418

21461

20621

2677245times1305

1919

2100

2197

2072

5700

5142

5347

5397

11687

10565

10737

10996

4318

3956

4244

4173

17386

1570

816085

16393

2777245times(77371times

BSEC

TCP

-1)

1851

1935

2020

1936

2577

2460

2838

2625

6989

4778

4636

5468

1631

1662

1999

1764

9566

7238

7474

8093

2877245times96AC

-93

1729

1780

2133

1881

5541

5058

5748

5449

11952

8174

9526

9884

3646

3587

4071

3768

17493

13232

15274

15333

2977245timesTo

go-II

2300

2508

2658

2489

3067

5431

5963

4820

11737

11021

1216

411641

1598

4053

4770

3474

14805

16452

1812

616461

3077245times99HS-18

1982

2019

2223

2075

3846

3119

4225

3730

7493

6720

6476

6896

3205

2578

2643

2809

11339

9839

1070

110626

12 Advances in Agriculture

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

31CV

J-2-5-3-1-3times1305

2406

2553

3067

2675

4178

4796

6281

5085

13822

11425

12252

12500

2472

3241

4907

3540

18000

16222

18532

17585

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

2142

2173

2238

2184

4522

5235

5640

5132

11673

9658

10248

10526

2614

3766

4178

3519

1619

514893

15888

15659

33CV

J-2-5-3-1-3times

96AC

-93

2350

2477

2600

2476

4676

4280

6090

5015

11975

9832

10868

10891

3097

2931

4445

3491

16651

14111

1695

815907

34CV

J-2-5-3-1-3times

Togo-II

2093

2666

2989

2583

4058

3727

5190

4325

12588

10717

11217

11507

2742

2570

4185

3166

16645

14443

16407

15832

35CV

J-2-5-3-1-3times

99HS-18

2028

2311

2386

2242

3287

2513

5356

3719

10055

9733

1036

410051

2004

1581

4251

2612

13342

12246

1572

01376

9

361305times(77371times

BSEC

TCP

-1)

2005

2173

2210

2129

3569

4692

5140

4467

11652

9826

10041

10507

2005

3665

4179

3283

15221

14518

1518

214974

371305times96AC

-93

1822

2003

2047

1957

5115

4023

4813

4650

9965

7675

7571

8404

3456

2647

3155

3086

15080

11698

12384

13054

381305timesTo

go-II

2545

2583

2644

2591

6885

7283

6795

6987

12265

11410

12583

12086

4500

6120

5350

5323

1915

018693

19378

19074

391305times99HS-18

2070

2408

2593

2357

5219

4332

4800

4784

1092

97677

8968

9192

3924

3332

3840

3699

1614

912009

1376

913976

40(77371times

BSEC

TCP

-1)

times96AC

-93

2056

2115

2157

2109

3158

4463

4717

4113

9166

7540

8100

8268

1880

3542

3347

2923

12324

12003

12817

12381

41(77371times

BSEC

TCP

-1)

timesTo

go-II

1868

2253

2450

2190

4422

6458

7038

5973

1415

312369

1379

713439

2291

4646

5586

4174

18575

18827

20835

19412

42(77371times

BSEC

TCP

-1)

times99HS-18

1995

2026

2209

2077

2720

2909

3543

3057

7392

4692

5023

5702

1766

2187

2147

2033

10112

7601

8566

8760

4396AC

-93timesTo

go-II

1953

2027

2511

2164

5972

4391

7126

5830

15537

1392

714432

14632

3780

2538

5655

3991

21510

18318

21558

20462

4496AC

-93times99HS-18

1952

2021

2242

2072

3465

2758

4496

3573

5959

5186

6094

5746

2707

2154

2409

2423

9424

7944

10590

9319

45To

go-IItimes

99HS-18

1932

2008

2658

2199

5381

4995

5436

5270

10299

8876

9244

9473

3986

3842

3668

3832

15680

13871

14680

1474

3Mean

1982

2146

2301

2143

3963

4123

4836

4307

9498

8087

8663

8749

2510

2914

3513

2979

13460

12210

13499

13056

SE243

198

245

mdash671

501

626

mdash959

750

822

mdash423

349

344

mdash1155

927

1167

mdashCD

087

071

087

mdash239

178

223

mdash342

267

293

mdash15

112

412

2mdash

411

330

416

mdash

Advances in Agriculture 13Ta

ble4Analysis

ofvaria

ncefor

10times10

half-diallelfor

different

charactersin

threee

nviro

nments

(a)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Germination

()

Rateof

emergence

STI()

SSTI

()

Num

bero

fleavesseedling

Seedlin

gheight

(cm)

Freshweight

(g)seedlin

gDry

weight

(g)seedlin

g

E 1

Replicates

2652731

596768

783393

20700

93018674

33764

1033999

002590

Treatm

ents

541097

0255lowastlowast

8181153lowastlowast

30863092lowastlowast

20680900lowastlowast

1100708lowastlowast

2106317lowastlowast

930829527lowastlowast

319767lowastlowast

Parents

95118

163lowastlowast

14289925lowastlowast

59114816lowastlowast

18880254lowastlowast

1084227lowastlowast

1087906lowastlowast

22561719lowastlowast

042162lowastlowast

Hybrid

s44

11423573lowastlowast

3398933lowastlowast

25697199lowastlowast

20633716lowastlowast

472874lowastlowast

1391526lowastlowast

674123518lowastlowast

261839lowastlowast

Parentsv

ersush

ybrid

s1

43693104lowastlowast

163619

916lowastlowast

3896838

3896

2793lowastlowast

28873741lowastlowast

4272

2798lowastlowast

234144289lowastlowast

5367022lowastlowast

Error

108

281184

1356832

1173684

674337

040104

306

462

2015

1619

005535

E 2

Replicates

2583819

3707389lowast

1596635

198738

034925

448213

899529

007177

Treatm

ents

549997443lowastlowast

2825177lowastlowast

35378177lowastlowast

19074306lowastlowast

665284lowastlowast

1764

081lowastlowast

257694570lowastlowast

2177519lowastlowast

Parents

96507784lowastlowast

3718

879lowastlowast

69866289lowastlowast

1798

5404lowastlowast

705379lowastlowast

1408518lowastlowast

10612519lowastlowast

077429lowastlowast

Hybrid

s44

10569731lowastlowast

2127302lowastlowast

2910

2537lowastlowast

19429240lowastlowast

329256lowastlowast

7000

03lowastlowast

186026552lowastlowast

1483428lowastlowast

Parentsv

ersush

ybrid

s1

16223710lowastlowast

25488361lowastlowast

1113

326

13257362lowastlowast

15089673lowastlowast

5178

3569lowastlowast

5634825791lowastlowast

51618348lowastlowast

Error

108

334760

1097039

1049505

635570

06764

116

0576

694613

006206

E 3

Replicates

2894398

4808815lowast

mdashmdash

051076

095989

3768421

030220

Treatm

ents

541261560

0lowastlowast

4842216lowastlowast

mdashmdash

2811077lowastlowast

5947316lowastlowast

3870844

50lowastlowast

3445076lowastlowast

Parents

97135

735lowastlowast

1097

6388lowastlowast

mdashmdash

1468699lowastlowast

1112

764lowastlowast

136081650lowastlowast

1307836lowastlowast

Hybrid

s44

13217740lowastlowast

1379360

mdashmdash

15540

71lowastlowast

4581632lowastlowast

252084767lowastlowast

2378032lowastlowast

Parentsv

ersush

ybrid

s1

35440

180lowastlowast

102000335lowastlowast

mdashmdash

70200767lowastlowast

109548362lowastlowast

8586095705lowastlowast

69630188lowastlowast

Error

108

782907

1085444

mdashmdash

139771

291325

3019

169

02400

4(b)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Panicle

emergence

Effectiv

etillersplant

Planth

eight

Earlength

Earw

eightp

lant

Dry

fodd

eryieldplant

Grain

yieldplant

Totalbiological

yieldplant

E 1

Replicates

231860

61lowastlowast

416348lowastlowast

1672

9472lowast

1706

411077192

9634539

392777

115544

59Treatm

ents

544152

368lowastlowast

085657lowastlowast

326706089lowastlowast

1602572lowastlowast

6177

0406lowastlowast

304113

204lowastlowast

34285030lowastlowast

575617

582lowastlowast

Parents

94511481lowastlowast

018538lowastlowast

140807476lowastlowast

2240093lowastlowast

11653

959lowastlowast

27014155lowastlowast

3858650lowastlowast

61321919lowastlowast

Hybrid

s44

3201347lowastlowast

100797lowastlowast

110395657lowastlowast

1010

497lowastlowast

40296671lowastlowast

171271411lowastlowast

2413

6880lowastlowast

302999650lowastlowast

Parentsv

ersush

ybrid

s1

4276

5286lowastlowast

02360

9lowast11517452

616lowastlowast

2191

6167lowastlowast

1457662749lowastlowast

8643043524lowastlowast

7546

41053lowastlowast

17199467546lowastlowast

Error

108

468159

004907

3692099

224610

17114

303498126

682350

5075268

E 2

Replicates

2656364

002556

61575565lowastlowast

828842lowastlowast

589094

2268506

260877

1507116

Treatm

ents

542999618lowastlowast

069822lowastlowast

268998723lowastlowast

2436886lowastlowast

60592784lowastlowast

247259877lowastlowast

4192

0073lowastlowast

513483489lowastlowast

Parents

926300

00lowastlowast

048079lowastlowast

165555

405lowastlowast

3871874lowastlowast

1412

2393lowastlowast

19543682lowastlowast

3132

426lowastlowast

57230706lowastlowast

Hybrid

s44

2533

165lowastlowast

07344

9lowastlowast

9616

2857lowastlowast

1463359lowastlowast

35495366lowastlowast

154759288lowastlowast

26262465lowastlowast

293492304lowastlowast

Parentsv

ersush

ybrid

s1

26850135lowastlowast

105929lowastlowast

880476

6697lowastlowast

32357161lowastlowast

1583112746lowastlowast

6366731560lowastlowast

107994

3646lowastlowast

14299370699lowastlowast

Error

108

507598

005829

5234122

149406

955133

2142411

462311

3273886

E 3

Replicates

2029697

021161

9459847

143746

985141

46800

6492

6334

3345160

Treatm

ents

548045499lowastlowast

094511lowastlowast

192420108lowastlowast

3279225lowastlowast

72049211lowastlowast

298953

021lowastlowast

55061308lowastlowast

651732994lowastlowast

Parents

98010

741lowastlowast

094166lowastlowast

116037343lowastlowast

3702185lowastlowast

16305190lowastlowast

2323644

0lowastlowast

4553

893lowastlowast

71484982lowastlowast

Hybrid

s44

6254377lowastlowast

095514lowastlowast

7378

1513lowastlowast

2188348lowastlowast

31813193lowastlowast

190263916lowastlowast

31572179lowastlowast

368689934lowastlowast

Parentsv

ersush

ybrid

s1

8716

7710lowastlowast

053496lowast

609996

3199lowastlowast

47471212lowastlowast

2344

130203lowastlowast

756272

2861lowastlowast

154314

9707lowastlowast

18327859

719lowastlowast

Error

108

696981

007883

5578505

228608

1491014

2574

223

449588

5187705

lowast

Sign

ificant

at119875=005

lowastlowastSign

ificant

at119875=001

14 Advances in Agriculture

Table 5 Mean performance of the genotypes for membrane thermostability in two environments

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594

Advances in Agriculture 15

Table 5 Continued

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434

Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash

lowastValues are transformed values

0

6

12

18

24

30

36

42

48

54

minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity

G73-10796AC-93CVJ-2-5-3-1-3

Togo-II99HS-18

H77833-2H7729-2772451305

H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305

1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305

H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305

77245 times 1305

77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305

77371 times BSECT CP-1

H77833-2 times G73-107

Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits

moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling

survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The

16 Advances in Agriculture

Table6Po

oled

correla

tioncoeffi

cientamon

gvario

uscharactersin

threee

nviro

nments

Characters

Germination

Rateof

emergence

STI

SSTI

Leaves

seedlin

gSeedlin

gheight

Fresh

weight

Dry

weight

MTS

Panicle

emergence

Effectiv

etillersplant

Plant

height

Earlength

Ear

weightp

lant

Dry

fodd

eryield

plant

Grain

yield

plant

Total

biological

yield

plant

Germination

1000

0357lowastlowast

0353lowastlowast

0590lowastlowast

0230

0373lowastlowast

0293lowast

0260

0276lowastminus0135minus0478lowastlowast

0326lowast

minus0069minus0104

minus0190

minus0145

minus0167

Rateof

emergence

1000

0233

0351lowastlowast

0605lowastlowast

0585lowastlowast

0551lowastlowast

0524lowastlowast

0139minus0593lowastlowast

0032

0517lowastlowast

0217

0388lowastlowast

0361lowastlowast

0348lowastlowast

0377lowastlowast

STI

1000

0837lowastlowast

0241

0129

0081

004

80890lowastlowast

minus0215

minus0211

0030minus0007minus0043

minus0101

minus0111

minus0084

SSTI

1000

0233

0264

0183

0140

0745lowastlowast

minus0247minus0268lowast

0184minus0017minus006

6minus0129

minus0144

minus0112

Num

bero

fleaves

1000

0683lowastlowast

0633lowastlowast

060

4lowastlowast

0176minus0474lowastlowast

0109

0576lowastlowast

0496lowastlowast

064

1lowastlowast

0607lowastlowast

0630lowastlowast

0630lowastlowast

Seedlin

gheight

1000

0703lowastlowast

0663lowastlowast

0031minus0493lowastlowast

minus0115

0735lowastlowast

0360lowastlowast

0526lowastlowast

0520lowastlowast

0515lowastlowast

0532lowastlowast

Freshweight

1000

0957lowastlowast

minus0016

0425lowastlowast

minus0082

0661lowastlowast

0351lowastlowast

0581lowastlowast

0496lowastlowast

0576lowastlowast

0533lowastlowast

Dry

weight

1000minus0033minus0428lowastlowast

minus0016

0634lowastlowast

0354lowastlowast

0620lowastlowast

0509lowastlowast

0614lowastlowast

0554lowastlowast

MTS

1000minus040

minus0177minus0037

0015

minus0074

minus0138

minus0131

minus0120

Panicle

emergence

1000

minus0150minus0269lowast

0022minus0329lowast

minus0337lowast

minus0280lowast

minus0341lowast

Effectiv

etillersplant

1000

minus0012

0170

0366lowastlowast

040

5lowastlowast

0323lowast

0401lowastlowast

Planth

eight

1000

0650lowastlowast

0603lowastlowast

0596lowastlowast

0619lowastlowast

0610lowastlowast

Earlength

1000

0608lowastlowast

0671lowastlowast

064

8lowastlowast

066

4lowastlowast

Earw

eightp

lant

1000

0910lowastlowast

0984lowastlowast

0957lowastlowast

Dry

fodd

eryield

plant

1000

0907lowastlowast

0991lowastlowast

Grain

yield

plant

1000

0950lowastlowast

Totalbiological

yield

plant

1000

lowastSign

ificant

at119875=005lowastlowastSign

ificant

at119875=001

Advances in Agriculture 17

faster germination and early vigour in seedling could act asphysical indicators for heat tolerance

Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits

Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics

Conflict of Interests

The authors declare that there is no conflict of interestsregarding the publication of this paper

References

[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981

[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993

[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006

[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993

[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013

[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007

[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965

[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011

[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001

[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009

[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962

[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967

[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963

[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001

Submit your manuscripts athttpwwwhindawicom

Nutrition and Metabolism

Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Food ScienceInternational Journal of

Agronomy

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Microbiology

The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom

Applied ampEnvironmentalSoil Science

Volume 2014

AgricultureAdvances in

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

BiodiversityInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

GenomicsInternational Journal of

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Plant GenomicsInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Biotechnology Research International

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Forestry ResearchInternational Journal of

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Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

EcologyInternational Journal of

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Page 12: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

12 Advances in Agriculture

(c)Con

tinued

Sr

No

Genotypes

Earheadleng

th(cm)

Earheadweight(g)plant

Dry

fodd

eryield(g)plant

Grain

yield(g)plant

Totalbiologicalyield

(g)plant

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

E 1E 2

E 3Mean

31CV

J-2-5-3-1-3times1305

2406

2553

3067

2675

4178

4796

6281

5085

13822

11425

12252

12500

2472

3241

4907

3540

18000

16222

18532

17585

32CV

J-2-5-3-1-3times

(77371times

BSEC

TCP

-1)

2142

2173

2238

2184

4522

5235

5640

5132

11673

9658

10248

10526

2614

3766

4178

3519

1619

514893

15888

15659

33CV

J-2-5-3-1-3times

96AC

-93

2350

2477

2600

2476

4676

4280

6090

5015

11975

9832

10868

10891

3097

2931

4445

3491

16651

14111

1695

815907

34CV

J-2-5-3-1-3times

Togo-II

2093

2666

2989

2583

4058

3727

5190

4325

12588

10717

11217

11507

2742

2570

4185

3166

16645

14443

16407

15832

35CV

J-2-5-3-1-3times

99HS-18

2028

2311

2386

2242

3287

2513

5356

3719

10055

9733

1036

410051

2004

1581

4251

2612

13342

12246

1572

01376

9

361305times(77371times

BSEC

TCP

-1)

2005

2173

2210

2129

3569

4692

5140

4467

11652

9826

10041

10507

2005

3665

4179

3283

15221

14518

1518

214974

371305times96AC

-93

1822

2003

2047

1957

5115

4023

4813

4650

9965

7675

7571

8404

3456

2647

3155

3086

15080

11698

12384

13054

381305timesTo

go-II

2545

2583

2644

2591

6885

7283

6795

6987

12265

11410

12583

12086

4500

6120

5350

5323

1915

018693

19378

19074

391305times99HS-18

2070

2408

2593

2357

5219

4332

4800

4784

1092

97677

8968

9192

3924

3332

3840

3699

1614

912009

1376

913976

40(77371times

BSEC

TCP

-1)

times96AC

-93

2056

2115

2157

2109

3158

4463

4717

4113

9166

7540

8100

8268

1880

3542

3347

2923

12324

12003

12817

12381

41(77371times

BSEC

TCP

-1)

timesTo

go-II

1868

2253

2450

2190

4422

6458

7038

5973

1415

312369

1379

713439

2291

4646

5586

4174

18575

18827

20835

19412

42(77371times

BSEC

TCP

-1)

times99HS-18

1995

2026

2209

2077

2720

2909

3543

3057

7392

4692

5023

5702

1766

2187

2147

2033

10112

7601

8566

8760

4396AC

-93timesTo

go-II

1953

2027

2511

2164

5972

4391

7126

5830

15537

1392

714432

14632

3780

2538

5655

3991

21510

18318

21558

20462

4496AC

-93times99HS-18

1952

2021

2242

2072

3465

2758

4496

3573

5959

5186

6094

5746

2707

2154

2409

2423

9424

7944

10590

9319

45To

go-IItimes

99HS-18

1932

2008

2658

2199

5381

4995

5436

5270

10299

8876

9244

9473

3986

3842

3668

3832

15680

13871

14680

1474

3Mean

1982

2146

2301

2143

3963

4123

4836

4307

9498

8087

8663

8749

2510

2914

3513

2979

13460

12210

13499

13056

SE243

198

245

mdash671

501

626

mdash959

750

822

mdash423

349

344

mdash1155

927

1167

mdashCD

087

071

087

mdash239

178

223

mdash342

267

293

mdash15

112

412

2mdash

411

330

416

mdash

Advances in Agriculture 13Ta

ble4Analysis

ofvaria

ncefor

10times10

half-diallelfor

different

charactersin

threee

nviro

nments

(a)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Germination

()

Rateof

emergence

STI()

SSTI

()

Num

bero

fleavesseedling

Seedlin

gheight

(cm)

Freshweight

(g)seedlin

gDry

weight

(g)seedlin

g

E 1

Replicates

2652731

596768

783393

20700

93018674

33764

1033999

002590

Treatm

ents

541097

0255lowastlowast

8181153lowastlowast

30863092lowastlowast

20680900lowastlowast

1100708lowastlowast

2106317lowastlowast

930829527lowastlowast

319767lowastlowast

Parents

95118

163lowastlowast

14289925lowastlowast

59114816lowastlowast

18880254lowastlowast

1084227lowastlowast

1087906lowastlowast

22561719lowastlowast

042162lowastlowast

Hybrid

s44

11423573lowastlowast

3398933lowastlowast

25697199lowastlowast

20633716lowastlowast

472874lowastlowast

1391526lowastlowast

674123518lowastlowast

261839lowastlowast

Parentsv

ersush

ybrid

s1

43693104lowastlowast

163619

916lowastlowast

3896838

3896

2793lowastlowast

28873741lowastlowast

4272

2798lowastlowast

234144289lowastlowast

5367022lowastlowast

Error

108

281184

1356832

1173684

674337

040104

306

462

2015

1619

005535

E 2

Replicates

2583819

3707389lowast

1596635

198738

034925

448213

899529

007177

Treatm

ents

549997443lowastlowast

2825177lowastlowast

35378177lowastlowast

19074306lowastlowast

665284lowastlowast

1764

081lowastlowast

257694570lowastlowast

2177519lowastlowast

Parents

96507784lowastlowast

3718

879lowastlowast

69866289lowastlowast

1798

5404lowastlowast

705379lowastlowast

1408518lowastlowast

10612519lowastlowast

077429lowastlowast

Hybrid

s44

10569731lowastlowast

2127302lowastlowast

2910

2537lowastlowast

19429240lowastlowast

329256lowastlowast

7000

03lowastlowast

186026552lowastlowast

1483428lowastlowast

Parentsv

ersush

ybrid

s1

16223710lowastlowast

25488361lowastlowast

1113

326

13257362lowastlowast

15089673lowastlowast

5178

3569lowastlowast

5634825791lowastlowast

51618348lowastlowast

Error

108

334760

1097039

1049505

635570

06764

116

0576

694613

006206

E 3

Replicates

2894398

4808815lowast

mdashmdash

051076

095989

3768421

030220

Treatm

ents

541261560

0lowastlowast

4842216lowastlowast

mdashmdash

2811077lowastlowast

5947316lowastlowast

3870844

50lowastlowast

3445076lowastlowast

Parents

97135

735lowastlowast

1097

6388lowastlowast

mdashmdash

1468699lowastlowast

1112

764lowastlowast

136081650lowastlowast

1307836lowastlowast

Hybrid

s44

13217740lowastlowast

1379360

mdashmdash

15540

71lowastlowast

4581632lowastlowast

252084767lowastlowast

2378032lowastlowast

Parentsv

ersush

ybrid

s1

35440

180lowastlowast

102000335lowastlowast

mdashmdash

70200767lowastlowast

109548362lowastlowast

8586095705lowastlowast

69630188lowastlowast

Error

108

782907

1085444

mdashmdash

139771

291325

3019

169

02400

4(b)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Panicle

emergence

Effectiv

etillersplant

Planth

eight

Earlength

Earw

eightp

lant

Dry

fodd

eryieldplant

Grain

yieldplant

Totalbiological

yieldplant

E 1

Replicates

231860

61lowastlowast

416348lowastlowast

1672

9472lowast

1706

411077192

9634539

392777

115544

59Treatm

ents

544152

368lowastlowast

085657lowastlowast

326706089lowastlowast

1602572lowastlowast

6177

0406lowastlowast

304113

204lowastlowast

34285030lowastlowast

575617

582lowastlowast

Parents

94511481lowastlowast

018538lowastlowast

140807476lowastlowast

2240093lowastlowast

11653

959lowastlowast

27014155lowastlowast

3858650lowastlowast

61321919lowastlowast

Hybrid

s44

3201347lowastlowast

100797lowastlowast

110395657lowastlowast

1010

497lowastlowast

40296671lowastlowast

171271411lowastlowast

2413

6880lowastlowast

302999650lowastlowast

Parentsv

ersush

ybrid

s1

4276

5286lowastlowast

02360

9lowast11517452

616lowastlowast

2191

6167lowastlowast

1457662749lowastlowast

8643043524lowastlowast

7546

41053lowastlowast

17199467546lowastlowast

Error

108

468159

004907

3692099

224610

17114

303498126

682350

5075268

E 2

Replicates

2656364

002556

61575565lowastlowast

828842lowastlowast

589094

2268506

260877

1507116

Treatm

ents

542999618lowastlowast

069822lowastlowast

268998723lowastlowast

2436886lowastlowast

60592784lowastlowast

247259877lowastlowast

4192

0073lowastlowast

513483489lowastlowast

Parents

926300

00lowastlowast

048079lowastlowast

165555

405lowastlowast

3871874lowastlowast

1412

2393lowastlowast

19543682lowastlowast

3132

426lowastlowast

57230706lowastlowast

Hybrid

s44

2533

165lowastlowast

07344

9lowastlowast

9616

2857lowastlowast

1463359lowastlowast

35495366lowastlowast

154759288lowastlowast

26262465lowastlowast

293492304lowastlowast

Parentsv

ersush

ybrid

s1

26850135lowastlowast

105929lowastlowast

880476

6697lowastlowast

32357161lowastlowast

1583112746lowastlowast

6366731560lowastlowast

107994

3646lowastlowast

14299370699lowastlowast

Error

108

507598

005829

5234122

149406

955133

2142411

462311

3273886

E 3

Replicates

2029697

021161

9459847

143746

985141

46800

6492

6334

3345160

Treatm

ents

548045499lowastlowast

094511lowastlowast

192420108lowastlowast

3279225lowastlowast

72049211lowastlowast

298953

021lowastlowast

55061308lowastlowast

651732994lowastlowast

Parents

98010

741lowastlowast

094166lowastlowast

116037343lowastlowast

3702185lowastlowast

16305190lowastlowast

2323644

0lowastlowast

4553

893lowastlowast

71484982lowastlowast

Hybrid

s44

6254377lowastlowast

095514lowastlowast

7378

1513lowastlowast

2188348lowastlowast

31813193lowastlowast

190263916lowastlowast

31572179lowastlowast

368689934lowastlowast

Parentsv

ersush

ybrid

s1

8716

7710lowastlowast

053496lowast

609996

3199lowastlowast

47471212lowastlowast

2344

130203lowastlowast

756272

2861lowastlowast

154314

9707lowastlowast

18327859

719lowastlowast

Error

108

696981

007883

5578505

228608

1491014

2574

223

449588

5187705

lowast

Sign

ificant

at119875=005

lowastlowastSign

ificant

at119875=001

14 Advances in Agriculture

Table 5 Mean performance of the genotypes for membrane thermostability in two environments

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594

Advances in Agriculture 15

Table 5 Continued

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434

Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash

lowastValues are transformed values

0

6

12

18

24

30

36

42

48

54

minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity

G73-10796AC-93CVJ-2-5-3-1-3

Togo-II99HS-18

H77833-2H7729-2772451305

H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305

1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305

H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305

77245 times 1305

77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305

77371 times BSECT CP-1

H77833-2 times G73-107

Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits

moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling

survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The

16 Advances in Agriculture

Table6Po

oled

correla

tioncoeffi

cientamon

gvario

uscharactersin

threee

nviro

nments

Characters

Germination

Rateof

emergence

STI

SSTI

Leaves

seedlin

gSeedlin

gheight

Fresh

weight

Dry

weight

MTS

Panicle

emergence

Effectiv

etillersplant

Plant

height

Earlength

Ear

weightp

lant

Dry

fodd

eryield

plant

Grain

yield

plant

Total

biological

yield

plant

Germination

1000

0357lowastlowast

0353lowastlowast

0590lowastlowast

0230

0373lowastlowast

0293lowast

0260

0276lowastminus0135minus0478lowastlowast

0326lowast

minus0069minus0104

minus0190

minus0145

minus0167

Rateof

emergence

1000

0233

0351lowastlowast

0605lowastlowast

0585lowastlowast

0551lowastlowast

0524lowastlowast

0139minus0593lowastlowast

0032

0517lowastlowast

0217

0388lowastlowast

0361lowastlowast

0348lowastlowast

0377lowastlowast

STI

1000

0837lowastlowast

0241

0129

0081

004

80890lowastlowast

minus0215

minus0211

0030minus0007minus0043

minus0101

minus0111

minus0084

SSTI

1000

0233

0264

0183

0140

0745lowastlowast

minus0247minus0268lowast

0184minus0017minus006

6minus0129

minus0144

minus0112

Num

bero

fleaves

1000

0683lowastlowast

0633lowastlowast

060

4lowastlowast

0176minus0474lowastlowast

0109

0576lowastlowast

0496lowastlowast

064

1lowastlowast

0607lowastlowast

0630lowastlowast

0630lowastlowast

Seedlin

gheight

1000

0703lowastlowast

0663lowastlowast

0031minus0493lowastlowast

minus0115

0735lowastlowast

0360lowastlowast

0526lowastlowast

0520lowastlowast

0515lowastlowast

0532lowastlowast

Freshweight

1000

0957lowastlowast

minus0016

0425lowastlowast

minus0082

0661lowastlowast

0351lowastlowast

0581lowastlowast

0496lowastlowast

0576lowastlowast

0533lowastlowast

Dry

weight

1000minus0033minus0428lowastlowast

minus0016

0634lowastlowast

0354lowastlowast

0620lowastlowast

0509lowastlowast

0614lowastlowast

0554lowastlowast

MTS

1000minus040

minus0177minus0037

0015

minus0074

minus0138

minus0131

minus0120

Panicle

emergence

1000

minus0150minus0269lowast

0022minus0329lowast

minus0337lowast

minus0280lowast

minus0341lowast

Effectiv

etillersplant

1000

minus0012

0170

0366lowastlowast

040

5lowastlowast

0323lowast

0401lowastlowast

Planth

eight

1000

0650lowastlowast

0603lowastlowast

0596lowastlowast

0619lowastlowast

0610lowastlowast

Earlength

1000

0608lowastlowast

0671lowastlowast

064

8lowastlowast

066

4lowastlowast

Earw

eightp

lant

1000

0910lowastlowast

0984lowastlowast

0957lowastlowast

Dry

fodd

eryield

plant

1000

0907lowastlowast

0991lowastlowast

Grain

yield

plant

1000

0950lowastlowast

Totalbiological

yield

plant

1000

lowastSign

ificant

at119875=005lowastlowastSign

ificant

at119875=001

Advances in Agriculture 17

faster germination and early vigour in seedling could act asphysical indicators for heat tolerance

Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits

Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics

Conflict of Interests

The authors declare that there is no conflict of interestsregarding the publication of this paper

References

[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981

[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993

[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006

[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993

[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013

[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007

[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965

[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011

[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001

[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009

[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962

[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967

[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963

[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001

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Page 13: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

Advances in Agriculture 13Ta

ble4Analysis

ofvaria

ncefor

10times10

half-diallelfor

different

charactersin

threee

nviro

nments

(a)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Germination

()

Rateof

emergence

STI()

SSTI

()

Num

bero

fleavesseedling

Seedlin

gheight

(cm)

Freshweight

(g)seedlin

gDry

weight

(g)seedlin

g

E 1

Replicates

2652731

596768

783393

20700

93018674

33764

1033999

002590

Treatm

ents

541097

0255lowastlowast

8181153lowastlowast

30863092lowastlowast

20680900lowastlowast

1100708lowastlowast

2106317lowastlowast

930829527lowastlowast

319767lowastlowast

Parents

95118

163lowastlowast

14289925lowastlowast

59114816lowastlowast

18880254lowastlowast

1084227lowastlowast

1087906lowastlowast

22561719lowastlowast

042162lowastlowast

Hybrid

s44

11423573lowastlowast

3398933lowastlowast

25697199lowastlowast

20633716lowastlowast

472874lowastlowast

1391526lowastlowast

674123518lowastlowast

261839lowastlowast

Parentsv

ersush

ybrid

s1

43693104lowastlowast

163619

916lowastlowast

3896838

3896

2793lowastlowast

28873741lowastlowast

4272

2798lowastlowast

234144289lowastlowast

5367022lowastlowast

Error

108

281184

1356832

1173684

674337

040104

306

462

2015

1619

005535

E 2

Replicates

2583819

3707389lowast

1596635

198738

034925

448213

899529

007177

Treatm

ents

549997443lowastlowast

2825177lowastlowast

35378177lowastlowast

19074306lowastlowast

665284lowastlowast

1764

081lowastlowast

257694570lowastlowast

2177519lowastlowast

Parents

96507784lowastlowast

3718

879lowastlowast

69866289lowastlowast

1798

5404lowastlowast

705379lowastlowast

1408518lowastlowast

10612519lowastlowast

077429lowastlowast

Hybrid

s44

10569731lowastlowast

2127302lowastlowast

2910

2537lowastlowast

19429240lowastlowast

329256lowastlowast

7000

03lowastlowast

186026552lowastlowast

1483428lowastlowast

Parentsv

ersush

ybrid

s1

16223710lowastlowast

25488361lowastlowast

1113

326

13257362lowastlowast

15089673lowastlowast

5178

3569lowastlowast

5634825791lowastlowast

51618348lowastlowast

Error

108

334760

1097039

1049505

635570

06764

116

0576

694613

006206

E 3

Replicates

2894398

4808815lowast

mdashmdash

051076

095989

3768421

030220

Treatm

ents

541261560

0lowastlowast

4842216lowastlowast

mdashmdash

2811077lowastlowast

5947316lowastlowast

3870844

50lowastlowast

3445076lowastlowast

Parents

97135

735lowastlowast

1097

6388lowastlowast

mdashmdash

1468699lowastlowast

1112

764lowastlowast

136081650lowastlowast

1307836lowastlowast

Hybrid

s44

13217740lowastlowast

1379360

mdashmdash

15540

71lowastlowast

4581632lowastlowast

252084767lowastlowast

2378032lowastlowast

Parentsv

ersush

ybrid

s1

35440

180lowastlowast

102000335lowastlowast

mdashmdash

70200767lowastlowast

109548362lowastlowast

8586095705lowastlowast

69630188lowastlowast

Error

108

782907

1085444

mdashmdash

139771

291325

3019

169

02400

4(b)

Environm

ent

Source

ofvaria

tion

DF

Meansum

ofsquares

Panicle

emergence

Effectiv

etillersplant

Planth

eight

Earlength

Earw

eightp

lant

Dry

fodd

eryieldplant

Grain

yieldplant

Totalbiological

yieldplant

E 1

Replicates

231860

61lowastlowast

416348lowastlowast

1672

9472lowast

1706

411077192

9634539

392777

115544

59Treatm

ents

544152

368lowastlowast

085657lowastlowast

326706089lowastlowast

1602572lowastlowast

6177

0406lowastlowast

304113

204lowastlowast

34285030lowastlowast

575617

582lowastlowast

Parents

94511481lowastlowast

018538lowastlowast

140807476lowastlowast

2240093lowastlowast

11653

959lowastlowast

27014155lowastlowast

3858650lowastlowast

61321919lowastlowast

Hybrid

s44

3201347lowastlowast

100797lowastlowast

110395657lowastlowast

1010

497lowastlowast

40296671lowastlowast

171271411lowastlowast

2413

6880lowastlowast

302999650lowastlowast

Parentsv

ersush

ybrid

s1

4276

5286lowastlowast

02360

9lowast11517452

616lowastlowast

2191

6167lowastlowast

1457662749lowastlowast

8643043524lowastlowast

7546

41053lowastlowast

17199467546lowastlowast

Error

108

468159

004907

3692099

224610

17114

303498126

682350

5075268

E 2

Replicates

2656364

002556

61575565lowastlowast

828842lowastlowast

589094

2268506

260877

1507116

Treatm

ents

542999618lowastlowast

069822lowastlowast

268998723lowastlowast

2436886lowastlowast

60592784lowastlowast

247259877lowastlowast

4192

0073lowastlowast

513483489lowastlowast

Parents

926300

00lowastlowast

048079lowastlowast

165555

405lowastlowast

3871874lowastlowast

1412

2393lowastlowast

19543682lowastlowast

3132

426lowastlowast

57230706lowastlowast

Hybrid

s44

2533

165lowastlowast

07344

9lowastlowast

9616

2857lowastlowast

1463359lowastlowast

35495366lowastlowast

154759288lowastlowast

26262465lowastlowast

293492304lowastlowast

Parentsv

ersush

ybrid

s1

26850135lowastlowast

105929lowastlowast

880476

6697lowastlowast

32357161lowastlowast

1583112746lowastlowast

6366731560lowastlowast

107994

3646lowastlowast

14299370699lowastlowast

Error

108

507598

005829

5234122

149406

955133

2142411

462311

3273886

E 3

Replicates

2029697

021161

9459847

143746

985141

46800

6492

6334

3345160

Treatm

ents

548045499lowastlowast

094511lowastlowast

192420108lowastlowast

3279225lowastlowast

72049211lowastlowast

298953

021lowastlowast

55061308lowastlowast

651732994lowastlowast

Parents

98010

741lowastlowast

094166lowastlowast

116037343lowastlowast

3702185lowastlowast

16305190lowastlowast

2323644

0lowastlowast

4553

893lowastlowast

71484982lowastlowast

Hybrid

s44

6254377lowastlowast

095514lowastlowast

7378

1513lowastlowast

2188348lowastlowast

31813193lowastlowast

190263916lowastlowast

31572179lowastlowast

368689934lowastlowast

Parentsv

ersush

ybrid

s1

8716

7710lowastlowast

053496lowast

609996

3199lowastlowast

47471212lowastlowast

2344

130203lowastlowast

756272

2861lowastlowast

154314

9707lowastlowast

18327859

719lowastlowast

Error

108

696981

007883

5578505

228608

1491014

2574

223

449588

5187705

lowast

Sign

ificant

at119875=005

lowastlowastSign

ificant

at119875=001

14 Advances in Agriculture

Table 5 Mean performance of the genotypes for membrane thermostability in two environments

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594

Advances in Agriculture 15

Table 5 Continued

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434

Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash

lowastValues are transformed values

0

6

12

18

24

30

36

42

48

54

minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity

G73-10796AC-93CVJ-2-5-3-1-3

Togo-II99HS-18

H77833-2H7729-2772451305

H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305

1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305

H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305

77245 times 1305

77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305

77371 times BSECT CP-1

H77833-2 times G73-107

Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits

moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling

survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The

16 Advances in Agriculture

Table6Po

oled

correla

tioncoeffi

cientamon

gvario

uscharactersin

threee

nviro

nments

Characters

Germination

Rateof

emergence

STI

SSTI

Leaves

seedlin

gSeedlin

gheight

Fresh

weight

Dry

weight

MTS

Panicle

emergence

Effectiv

etillersplant

Plant

height

Earlength

Ear

weightp

lant

Dry

fodd

eryield

plant

Grain

yield

plant

Total

biological

yield

plant

Germination

1000

0357lowastlowast

0353lowastlowast

0590lowastlowast

0230

0373lowastlowast

0293lowast

0260

0276lowastminus0135minus0478lowastlowast

0326lowast

minus0069minus0104

minus0190

minus0145

minus0167

Rateof

emergence

1000

0233

0351lowastlowast

0605lowastlowast

0585lowastlowast

0551lowastlowast

0524lowastlowast

0139minus0593lowastlowast

0032

0517lowastlowast

0217

0388lowastlowast

0361lowastlowast

0348lowastlowast

0377lowastlowast

STI

1000

0837lowastlowast

0241

0129

0081

004

80890lowastlowast

minus0215

minus0211

0030minus0007minus0043

minus0101

minus0111

minus0084

SSTI

1000

0233

0264

0183

0140

0745lowastlowast

minus0247minus0268lowast

0184minus0017minus006

6minus0129

minus0144

minus0112

Num

bero

fleaves

1000

0683lowastlowast

0633lowastlowast

060

4lowastlowast

0176minus0474lowastlowast

0109

0576lowastlowast

0496lowastlowast

064

1lowastlowast

0607lowastlowast

0630lowastlowast

0630lowastlowast

Seedlin

gheight

1000

0703lowastlowast

0663lowastlowast

0031minus0493lowastlowast

minus0115

0735lowastlowast

0360lowastlowast

0526lowastlowast

0520lowastlowast

0515lowastlowast

0532lowastlowast

Freshweight

1000

0957lowastlowast

minus0016

0425lowastlowast

minus0082

0661lowastlowast

0351lowastlowast

0581lowastlowast

0496lowastlowast

0576lowastlowast

0533lowastlowast

Dry

weight

1000minus0033minus0428lowastlowast

minus0016

0634lowastlowast

0354lowastlowast

0620lowastlowast

0509lowastlowast

0614lowastlowast

0554lowastlowast

MTS

1000minus040

minus0177minus0037

0015

minus0074

minus0138

minus0131

minus0120

Panicle

emergence

1000

minus0150minus0269lowast

0022minus0329lowast

minus0337lowast

minus0280lowast

minus0341lowast

Effectiv

etillersplant

1000

minus0012

0170

0366lowastlowast

040

5lowastlowast

0323lowast

0401lowastlowast

Planth

eight

1000

0650lowastlowast

0603lowastlowast

0596lowastlowast

0619lowastlowast

0610lowastlowast

Earlength

1000

0608lowastlowast

0671lowastlowast

064

8lowastlowast

066

4lowastlowast

Earw

eightp

lant

1000

0910lowastlowast

0984lowastlowast

0957lowastlowast

Dry

fodd

eryield

plant

1000

0907lowastlowast

0991lowastlowast

Grain

yield

plant

1000

0950lowastlowast

Totalbiological

yield

plant

1000

lowastSign

ificant

at119875=005lowastlowastSign

ificant

at119875=001

Advances in Agriculture 17

faster germination and early vigour in seedling could act asphysical indicators for heat tolerance

Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits

Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics

Conflict of Interests

The authors declare that there is no conflict of interestsregarding the publication of this paper

References

[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981

[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993

[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006

[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993

[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013

[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007

[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965

[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011

[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001

[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009

[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962

[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967

[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963

[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001

Submit your manuscripts athttpwwwhindawicom

Nutrition and Metabolism

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Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

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The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014

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Applied ampEnvironmentalSoil Science

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AgricultureAdvances in

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PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

BiodiversityInternational Journal of

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ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

GenomicsInternational Journal of

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Plant GenomicsInternational Journal of

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Biotechnology Research International

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Page 14: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

14 Advances in Agriculture

Table 5 Mean performance of the genotypes for membrane thermostability in two environments

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean1 H77833-2 7474 7373 74232 H7729-2 5337 5542 54393 G73-107 7944 7237 75914 77245 5415 5442 54285 CVJ-2-5-3-1-3 7574 7681 76276 1305 5228 5169 51997 77371 times BSECT CP-1 7421 7358 73908 96AC-93 7222 7563 73929 Togo-II 7435 7598 751610 99HS-18 7639 7465 75521 H77833-2 timesH7729-2 7211 6866 70392 H77833-2 times G73-107 7529 7072 73003 H77833-2 times 77245 6670 6742 67064 H77833-2 times CVJ-2-5-3-1-3 7403 7178 72905 H77833-2 times 1305 5121 5171 51466 H77833-2 times (77371 times BSECT CP-1) 7358 7234 72967 H77833-2 times 96AC-93 7587 7585 75868 H77833-2 times Togo-II 6757 6708 67339 H77833-2 times 99HS-18 6596 6602 659910 H7729-2 times G73-107 6867 6601 673411 H7729-2 times 77245 6153 6402 627712 H7729-2 times CVJ-2-5-3-1-3 7598 7886 774213 H7729-2 times 1305 6873 6892 688214 H7729-2 times (77371 times BSECT CP-1) 6620 6518 656915 H7729-2 times 96AC-93 5990 5830 591016 H7729-2 times Togo-II 7006 6821 691317 H7729-2 times 99HS-18 6738 6507 662218 G73-107 times 77245 7336 7089 721219 G73-107 times CVJ-2-5-3-1-3 6837 6960 689920 G73-107 times 1305 7009 7430 721921 G73-107 times (77371 times BSECT CP-1) 7130 6894 701222 G73-107 times 96AC-93 6722 7009 686523 G73-107 times Togo-II 5749 5541 564524 G73-107 times 99HS-18 6795 7011 690325 77245 times CVJ-2-5-3-1-3 6021 5945 598326 77245 times 1305 5423 5399 541127 77245 times (77371 times BSECT CP-1) 5224 5217 522028 77245 times 96AC-93 5639 5424 553129 77245 times Togo-II 4996 5150 507330 77245 times 99HS-18 5970 6219 609531 CVJ-2-5-3-1-3 times 1305 6191 6431 631132 CVJ-2-5-3-1-3 times (77371 times BSECT CP-1) 6808 6411 660933 CVJ-2-5-3-1-3 times 96AC-93 7813 7633 772334 CVJ-2-5-3-1-3 times Togo-II 6738 6801 676935 CVJ-2-5-3-1-3 times 99HS-18 7453 7363 740836 1305 times (77371 times BSECT CP-1) 6488 6447 646837 1305 times 96AC-93 7612 7490 755138 1305 times Togo-II 6911 7112 701239 1305 times 99HS-18 7680 7507 7594

Advances in Agriculture 15

Table 5 Continued

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434

Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash

lowastValues are transformed values

0

6

12

18

24

30

36

42

48

54

minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity

G73-10796AC-93CVJ-2-5-3-1-3

Togo-II99HS-18

H77833-2H7729-2772451305

H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305

1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305

H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305

77245 times 1305

77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305

77371 times BSECT CP-1

H77833-2 times G73-107

Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits

moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling

survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The

16 Advances in Agriculture

Table6Po

oled

correla

tioncoeffi

cientamon

gvario

uscharactersin

threee

nviro

nments

Characters

Germination

Rateof

emergence

STI

SSTI

Leaves

seedlin

gSeedlin

gheight

Fresh

weight

Dry

weight

MTS

Panicle

emergence

Effectiv

etillersplant

Plant

height

Earlength

Ear

weightp

lant

Dry

fodd

eryield

plant

Grain

yield

plant

Total

biological

yield

plant

Germination

1000

0357lowastlowast

0353lowastlowast

0590lowastlowast

0230

0373lowastlowast

0293lowast

0260

0276lowastminus0135minus0478lowastlowast

0326lowast

minus0069minus0104

minus0190

minus0145

minus0167

Rateof

emergence

1000

0233

0351lowastlowast

0605lowastlowast

0585lowastlowast

0551lowastlowast

0524lowastlowast

0139minus0593lowastlowast

0032

0517lowastlowast

0217

0388lowastlowast

0361lowastlowast

0348lowastlowast

0377lowastlowast

STI

1000

0837lowastlowast

0241

0129

0081

004

80890lowastlowast

minus0215

minus0211

0030minus0007minus0043

minus0101

minus0111

minus0084

SSTI

1000

0233

0264

0183

0140

0745lowastlowast

minus0247minus0268lowast

0184minus0017minus006

6minus0129

minus0144

minus0112

Num

bero

fleaves

1000

0683lowastlowast

0633lowastlowast

060

4lowastlowast

0176minus0474lowastlowast

0109

0576lowastlowast

0496lowastlowast

064

1lowastlowast

0607lowastlowast

0630lowastlowast

0630lowastlowast

Seedlin

gheight

1000

0703lowastlowast

0663lowastlowast

0031minus0493lowastlowast

minus0115

0735lowastlowast

0360lowastlowast

0526lowastlowast

0520lowastlowast

0515lowastlowast

0532lowastlowast

Freshweight

1000

0957lowastlowast

minus0016

0425lowastlowast

minus0082

0661lowastlowast

0351lowastlowast

0581lowastlowast

0496lowastlowast

0576lowastlowast

0533lowastlowast

Dry

weight

1000minus0033minus0428lowastlowast

minus0016

0634lowastlowast

0354lowastlowast

0620lowastlowast

0509lowastlowast

0614lowastlowast

0554lowastlowast

MTS

1000minus040

minus0177minus0037

0015

minus0074

minus0138

minus0131

minus0120

Panicle

emergence

1000

minus0150minus0269lowast

0022minus0329lowast

minus0337lowast

minus0280lowast

minus0341lowast

Effectiv

etillersplant

1000

minus0012

0170

0366lowastlowast

040

5lowastlowast

0323lowast

0401lowastlowast

Planth

eight

1000

0650lowastlowast

0603lowastlowast

0596lowastlowast

0619lowastlowast

0610lowastlowast

Earlength

1000

0608lowastlowast

0671lowastlowast

064

8lowastlowast

066

4lowastlowast

Earw

eightp

lant

1000

0910lowastlowast

0984lowastlowast

0957lowastlowast

Dry

fodd

eryield

plant

1000

0907lowastlowast

0991lowastlowast

Grain

yield

plant

1000

0950lowastlowast

Totalbiological

yield

plant

1000

lowastSign

ificant

at119875=005lowastlowastSign

ificant

at119875=001

Advances in Agriculture 17

faster germination and early vigour in seedling could act asphysical indicators for heat tolerance

Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits

Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics

Conflict of Interests

The authors declare that there is no conflict of interestsregarding the publication of this paper

References

[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981

[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993

[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006

[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993

[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013

[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007

[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965

[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011

[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001

[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009

[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962

[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967

[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963

[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001

Submit your manuscripts athttpwwwhindawicom

Nutrition and Metabolism

Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Food ScienceInternational Journal of

Agronomy

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Microbiology

The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom

Applied ampEnvironmentalSoil Science

Volume 2014

AgricultureAdvances in

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

BiodiversityInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

GenomicsInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Plant GenomicsInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Biotechnology Research International

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Forestry ResearchInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

EcologyInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Veterinary Medicine International

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Cell BiologyInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Evolutionary BiologyInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Page 15: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

Advances in Agriculture 15

Table 5 Continued

Sr No Genotypes Membrane thermostabilitylowast

E1 E2 Mean40 (77371 times BSECT CP-1) times 96AC-93 7579 7462 752141 (77371 times BSECT CP-1) times Togo-II 7606 7873 774042 (77371 times BSECT CP-1) times 99HS-18 7506 7470 748843 96AC-93 times Togo-II 6881 6615 674844 96AC-93 times 99HS-18 7368 6927 714745 Togo-II times 99HS-18 6560 6308 6434

Mean 6778 6730 6754CD 320 360 mdashSE 113 127 mdashCV 235 266 mdash

lowastValues are transformed values

0

6

12

18

24

30

36

42

48

54

minus50 minus45 minus40 minus35 minus30 minus25 minus20 minus15 minus10 minus5Similarity

G73-10796AC-93CVJ-2-5-3-1-3

Togo-II99HS-18

H77833-2H7729-2772451305

H77833-2 times H7729-2CVJ-2-5-3-1-3 times 99HS-18H7729-2 times G73-107H7729-2 times 77245H77833-2 times (77371timesBSECT CP-1)G73-107 times (77371times BSECT CP-1)(77371 times BSECT CP-1)H77833-2 times 77245H77833-2 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times Togo-IIG73-107 times Togo-II1305 times (77371 times BSECT CP-1)CVJ-2-5-3-1-3 times 1305

1305 times Togo-IIH77833-2 times Togo-II77245 times CVJ-2-5-3-1-3CVJ-2-5-3-1-3 times (77371 timesBSECT CP-1)H77833-2 times 99HS-18H7729-2 times 96AC-9377245 times 96AC-9377245 times 99HS-18H7729-2 times (77371 times BSECT CP-1)H7729-2 times 99HS-18G73-107 times 77245G73-107 times CVJ-2-5-3-1-31305 times 99HS-18G73-107 times 1305

H77833-2 times 96AC-93H7729-2 times CVJ-2-5-3-1-3G73-107 times 96AC-93G73-107 times 99HS-1896AC-93 times 99HS-181305 times 96AC-93CVJ-2-5-3-1-3 times 96AC-93(77371 times BSECT CP-1)H7729-2 times 1305

77245 times 1305

77245 times (77371 times BSECT CP-1)96AC-93 times Togo-II77245 times Togo-IITogo-II times 99HS-18H7729-2 times Togo-II(77371 times BSECT CP-1)H77833-2 times 1305

77371 times BSECT CP-1

H77833-2 times G73-107

Figure 2 Dendrogram representing similarity among pearl millet genotypes (parents and hybrids) based on PAST cluster analysis ofEuclidean distance based on phenotypic traits

moisture was depleting near to the permanent wilting pointand drought stress could also come into play along with heatstress to further continue the experiment Since experimentproceeded without any stress after the establishment ofseedling [4] the pearl millet plants show great resilience andrecovery The main impact of supraoptimal temperature atgermination and seedling stage was observed on the seedling

survival and resulted in poor plant standpopulation of pearlmillet Poor crop stand reduces the productivity (fodder andgrain) drastically whereas after seedling establishment noimpact of supraoptimal temperature at seedling stage wasobserved on later growth stages Hence the supraoptimaltemperature at early seedling stage in pearl millet could haveno impact on its later growth and development stages The

16 Advances in Agriculture

Table6Po

oled

correla

tioncoeffi

cientamon

gvario

uscharactersin

threee

nviro

nments

Characters

Germination

Rateof

emergence

STI

SSTI

Leaves

seedlin

gSeedlin

gheight

Fresh

weight

Dry

weight

MTS

Panicle

emergence

Effectiv

etillersplant

Plant

height

Earlength

Ear

weightp

lant

Dry

fodd

eryield

plant

Grain

yield

plant

Total

biological

yield

plant

Germination

1000

0357lowastlowast

0353lowastlowast

0590lowastlowast

0230

0373lowastlowast

0293lowast

0260

0276lowastminus0135minus0478lowastlowast

0326lowast

minus0069minus0104

minus0190

minus0145

minus0167

Rateof

emergence

1000

0233

0351lowastlowast

0605lowastlowast

0585lowastlowast

0551lowastlowast

0524lowastlowast

0139minus0593lowastlowast

0032

0517lowastlowast

0217

0388lowastlowast

0361lowastlowast

0348lowastlowast

0377lowastlowast

STI

1000

0837lowastlowast

0241

0129

0081

004

80890lowastlowast

minus0215

minus0211

0030minus0007minus0043

minus0101

minus0111

minus0084

SSTI

1000

0233

0264

0183

0140

0745lowastlowast

minus0247minus0268lowast

0184minus0017minus006

6minus0129

minus0144

minus0112

Num

bero

fleaves

1000

0683lowastlowast

0633lowastlowast

060

4lowastlowast

0176minus0474lowastlowast

0109

0576lowastlowast

0496lowastlowast

064

1lowastlowast

0607lowastlowast

0630lowastlowast

0630lowastlowast

Seedlin

gheight

1000

0703lowastlowast

0663lowastlowast

0031minus0493lowastlowast

minus0115

0735lowastlowast

0360lowastlowast

0526lowastlowast

0520lowastlowast

0515lowastlowast

0532lowastlowast

Freshweight

1000

0957lowastlowast

minus0016

0425lowastlowast

minus0082

0661lowastlowast

0351lowastlowast

0581lowastlowast

0496lowastlowast

0576lowastlowast

0533lowastlowast

Dry

weight

1000minus0033minus0428lowastlowast

minus0016

0634lowastlowast

0354lowastlowast

0620lowastlowast

0509lowastlowast

0614lowastlowast

0554lowastlowast

MTS

1000minus040

minus0177minus0037

0015

minus0074

minus0138

minus0131

minus0120

Panicle

emergence

1000

minus0150minus0269lowast

0022minus0329lowast

minus0337lowast

minus0280lowast

minus0341lowast

Effectiv

etillersplant

1000

minus0012

0170

0366lowastlowast

040

5lowastlowast

0323lowast

0401lowastlowast

Planth

eight

1000

0650lowastlowast

0603lowastlowast

0596lowastlowast

0619lowastlowast

0610lowastlowast

Earlength

1000

0608lowastlowast

0671lowastlowast

064

8lowastlowast

066

4lowastlowast

Earw

eightp

lant

1000

0910lowastlowast

0984lowastlowast

0957lowastlowast

Dry

fodd

eryield

plant

1000

0907lowastlowast

0991lowastlowast

Grain

yield

plant

1000

0950lowastlowast

Totalbiological

yield

plant

1000

lowastSign

ificant

at119875=005lowastlowastSign

ificant

at119875=001

Advances in Agriculture 17

faster germination and early vigour in seedling could act asphysical indicators for heat tolerance

Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits

Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics

Conflict of Interests

The authors declare that there is no conflict of interestsregarding the publication of this paper

References

[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981

[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993

[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006

[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993

[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013

[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007

[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965

[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011

[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001

[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009

[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962

[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967

[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963

[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001

Submit your manuscripts athttpwwwhindawicom

Nutrition and Metabolism

Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Food ScienceInternational Journal of

Agronomy

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Microbiology

The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom

Applied ampEnvironmentalSoil Science

Volume 2014

AgricultureAdvances in

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

BiodiversityInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

GenomicsInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Plant GenomicsInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Biotechnology Research International

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Forestry ResearchInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

EcologyInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Veterinary Medicine International

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Cell BiologyInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Evolutionary BiologyInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Page 16: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

16 Advances in Agriculture

Table6Po

oled

correla

tioncoeffi

cientamon

gvario

uscharactersin

threee

nviro

nments

Characters

Germination

Rateof

emergence

STI

SSTI

Leaves

seedlin

gSeedlin

gheight

Fresh

weight

Dry

weight

MTS

Panicle

emergence

Effectiv

etillersplant

Plant

height

Earlength

Ear

weightp

lant

Dry

fodd

eryield

plant

Grain

yield

plant

Total

biological

yield

plant

Germination

1000

0357lowastlowast

0353lowastlowast

0590lowastlowast

0230

0373lowastlowast

0293lowast

0260

0276lowastminus0135minus0478lowastlowast

0326lowast

minus0069minus0104

minus0190

minus0145

minus0167

Rateof

emergence

1000

0233

0351lowastlowast

0605lowastlowast

0585lowastlowast

0551lowastlowast

0524lowastlowast

0139minus0593lowastlowast

0032

0517lowastlowast

0217

0388lowastlowast

0361lowastlowast

0348lowastlowast

0377lowastlowast

STI

1000

0837lowastlowast

0241

0129

0081

004

80890lowastlowast

minus0215

minus0211

0030minus0007minus0043

minus0101

minus0111

minus0084

SSTI

1000

0233

0264

0183

0140

0745lowastlowast

minus0247minus0268lowast

0184minus0017minus006

6minus0129

minus0144

minus0112

Num

bero

fleaves

1000

0683lowastlowast

0633lowastlowast

060

4lowastlowast

0176minus0474lowastlowast

0109

0576lowastlowast

0496lowastlowast

064

1lowastlowast

0607lowastlowast

0630lowastlowast

0630lowastlowast

Seedlin

gheight

1000

0703lowastlowast

0663lowastlowast

0031minus0493lowastlowast

minus0115

0735lowastlowast

0360lowastlowast

0526lowastlowast

0520lowastlowast

0515lowastlowast

0532lowastlowast

Freshweight

1000

0957lowastlowast

minus0016

0425lowastlowast

minus0082

0661lowastlowast

0351lowastlowast

0581lowastlowast

0496lowastlowast

0576lowastlowast

0533lowastlowast

Dry

weight

1000minus0033minus0428lowastlowast

minus0016

0634lowastlowast

0354lowastlowast

0620lowastlowast

0509lowastlowast

0614lowastlowast

0554lowastlowast

MTS

1000minus040

minus0177minus0037

0015

minus0074

minus0138

minus0131

minus0120

Panicle

emergence

1000

minus0150minus0269lowast

0022minus0329lowast

minus0337lowast

minus0280lowast

minus0341lowast

Effectiv

etillersplant

1000

minus0012

0170

0366lowastlowast

040

5lowastlowast

0323lowast

0401lowastlowast

Planth

eight

1000

0650lowastlowast

0603lowastlowast

0596lowastlowast

0619lowastlowast

0610lowastlowast

Earlength

1000

0608lowastlowast

0671lowastlowast

064

8lowastlowast

066

4lowastlowast

Earw

eightp

lant

1000

0910lowastlowast

0984lowastlowast

0957lowastlowast

Dry

fodd

eryield

plant

1000

0907lowastlowast

0991lowastlowast

Grain

yield

plant

1000

0950lowastlowast

Totalbiological

yield

plant

1000

lowastSign

ificant

at119875=005lowastlowastSign

ificant

at119875=001

Advances in Agriculture 17

faster germination and early vigour in seedling could act asphysical indicators for heat tolerance

Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits

Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics

Conflict of Interests

The authors declare that there is no conflict of interestsregarding the publication of this paper

References

[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981

[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993

[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006

[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993

[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013

[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007

[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965

[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011

[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001

[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009

[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962

[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967

[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963

[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001

Submit your manuscripts athttpwwwhindawicom

Nutrition and Metabolism

Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Food ScienceInternational Journal of

Agronomy

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Microbiology

The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom

Applied ampEnvironmentalSoil Science

Volume 2014

AgricultureAdvances in

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

BiodiversityInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

GenomicsInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Plant GenomicsInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Biotechnology Research International

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Forestry ResearchInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

EcologyInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Veterinary Medicine International

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Cell BiologyInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Evolutionary BiologyInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Page 17: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

Advances in Agriculture 17

faster germination and early vigour in seedling could act asphysical indicators for heat tolerance

Most of the maturity traits showed significant correlationamong themselves Panicle emergence had a significant butnegative correlation with plant height ear weightplant dryfodder yieldplant grain yieldplant and total biologicalyieldplant whereas grain yieldplant showed significant cor-relation with rate of emergence of seedlings number ofleavesseedling seedling height fresh weight of seedling dryweight of seedling among the seedling traits and numberof effective tillersplant plant height ear length ear weightplant dry fodder yieldplant and total biological yieldplantamong the maturity traits

Cluster analysis of the data (all the characters) basedon similarity among genotypes gave the relative positionof parents and hybrids in each group (Figure 2) The pearlmillet genotypes were broadly grouped into four clustersThe parents are grouped into separate cluster The resultingpopulations from selective crosses will be useful for deriv-ing quantitative data which can be applied to quantitativegenetics in order to understand the inheritance and basisof thermotolerance expression in pearl millet On similarlines quantification of thermotolerance variations in pearlmillet will contribute to the understanding of inheritance ofthermotolerance expression at seedling stage through use ofdata in quantitative genetics

Conflict of Interests

The authors declare that there is no conflict of interestsregarding the publication of this paper

References

[1] P Soman F R Bidinger J M Peacock and T S WalkerldquoSeedling establishmentmdasha preliminary survey taken up inAurepally during kharif 1981rdquo ICRISAT Internal ReportICRISAT Patancheru India 1981

[2] S Baljeet Supra-optimal temperature tolerance in pearl milletinheritance pattern of some adaptive traits at seedling stage [MScThesis] CCS HAU Hisar India 1993

[3] A K Yadav M S Narwal and B Singh ldquoField screeningtechnique for heat effect on seedlings of pearl milletrdquo in Pro-ceedings of the National Seminar on Transgenic Crops in IndianAgriculture Status Risk and Acceptance pp 109ndash112 CCSHAUHisar India January 2006

[4] J M Peacock P Soman R Jayachandran A V Rani C JHowarth and A Thomas ldquoEffect of high soil surface tempera-ture on seedling survival in pearl milletrdquo Experimental Agricul-ture vol 29 no 2 pp 215ndash225 1993

[5] A K Yadav M S Narwal and R K Arya ldquoEvaluation ofpearl millet (Pennisetum glaucum) genotypes and validation ofscreening methods for supra-optimal temperature tolerance atseedling stagerdquo Indian Journal of Agricultural Sciences vol 83no 3 pp 260ndash271 2013

[6] S Rajbir ldquoPerformance of diclofop-methyl to control weedsin wheat (Triticum aestivum) under different irrigation levelsrdquoIndian Journal of Agricultural Research vol 41 no 1 pp 75ndash782007

[7] C A Black Methods of Soil Analysis Part I Physical and Min-eralogical Properties American Society of Agronomy MadisonWis USA 1965

[8] A K Yadav M S Narwal and R K Arya ldquoGenetic dissectionof temperature tolerance in pearl millet (Pennisetum glaucum)rdquoIndian Journal of Agricultural Sciences vol 81 no 3 pp 203ndash2132011

[9] A M H Ibrahim and J S Quick ldquoGenetic control of hightemperature tolerance in wheat as measured by membranethermal stabilityrdquo Crop Science vol 41 no 5 pp 1405ndash14072001

[10] A K Yadav R K Arya and SM Narwal ldquoScreening for supra-optimal temperature tolerance through membrane thermo-stability in pearl millet (Pennisetum glaucum)rdquo Forage Researchvol 35 no 2 pp 85ndash90 2009

[11] J D Maguire ldquoSpeed of germinationmdashaid in selection andevaluation for seedling emergence and vigourrdquo Crop Sciencevol 2 no 2 pp 176ndash177 1962

[12] V G Panse and P V Sukhatme Statistical Method for Agricul-tural Workers ICAR New Delhi India 4th edition 1967

[13] R A Fisher and F Yates Statistical Tables for Biological Agri-cultural and Medical Research Oliver and Boyd EdinburghUK 6th edition 1963

[14] Oslash Hammer D A T Harper and P D Ryan ldquoPast paleontolog-ical statistics software package for education and data analysisrdquoPalaeontologia Electronica vol 4 no 1 2001

Submit your manuscripts athttpwwwhindawicom

Nutrition and Metabolism

Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Food ScienceInternational Journal of

Agronomy

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Microbiology

The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom

Applied ampEnvironmentalSoil Science

Volume 2014

AgricultureAdvances in

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

BiodiversityInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

GenomicsInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Plant GenomicsInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Biotechnology Research International

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Forestry ResearchInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

EcologyInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Veterinary Medicine International

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Cell BiologyInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Evolutionary BiologyInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Page 18: Research Article Screening of Pearl Millet F Hybrids for ...downloads.hindawi.com/journals/aag/2014/231301.pdf · Research Article Screening of Pearl Millet F 1 Hybrids for Heat Tolerance

Submit your manuscripts athttpwwwhindawicom

Nutrition and Metabolism

Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Food ScienceInternational Journal of

Agronomy

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

International Journal of

Microbiology

The Scientific World JournalHindawi Publishing Corporation httpwwwhindawicom Volume 2014

Hindawi Publishing Corporationhttpwwwhindawicom

Applied ampEnvironmentalSoil Science

Volume 2014

AgricultureAdvances in

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

PsycheHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

BiodiversityInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

ScientificaHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

GenomicsInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Plant GenomicsInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Biotechnology Research International

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Forestry ResearchInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Journal of BotanyHindawi Publishing Corporationhttpwwwhindawicom Volume 2014

EcologyInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Veterinary Medicine International

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Cell BiologyInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014

Evolutionary BiologyInternational Journal of

Hindawi Publishing Corporationhttpwwwhindawicom Volume 2014