RESEARCH ASSISTANTTRAINING PROGRAM

ORGANIZED BY DRMC,

LABORATORY RESEARCH

byPn. Helen Ng Lee

Ching/ Pn. Intan Suhana

LABORATORY RESEARCH

• LAB INTRODUCTIONBRL – BIOMATERIAL RESEACRH LAB

(Level 7, Postgraduate & Research Tower)Research focus area:Properties, synthesis & characterization of biomaterials in dental

application.Services : Imaging, surface, texture analyzer, hardness tester,

compression & tensile test as well as grinding & polishing of dental materials.

Person in charge: Pn. Helen-Science Officer, Tel no. 03-79676460

LABORATORY RESEARCH

Services/Facilities – BRL3D OPTICAL SURFACETEXTURE ANALYZER

ATOMIC FORCEMICROSCOPE (AFM)

DISPERSIVE RAMAN SPECTROMETRE

DYNAMIC ULTRA- MICRO HARDNESS TESTING MACHINE

FOURIER TRANSFORM INFRARED (FTIR)

FULLY AUTOMATED VICKERS MICRO HARDNESS TESTING MACHINE

HIGH SPEED PRECISION SAWING MACHINE

LOW SPEED PRECISION SAWING MACHINE

SCANNING ELECTRONMICROSCOPE (SEM)

STEREO MICROSCOPE (3 UNITS)

THERMO – CYCLING MACHINE ( 2 UNITS )

THERMOMECHANICAL ANALYZER (TMA)

THIN SECTIONING MACHINE

UNIVERSAL TESTING MACHINE (UTM)

LABORATORY RESEARCH

• LAB INTRODUCTIONCMBRL – CRANIOFACIAL & MOLECULAR BIOLOGY

RESEARCH LAB (Level 7, Postgraduate & Research Tower)

Research focus area:Bio banking of oral cancer sample,research involving the

molecular basis of oral disease, stem cell, tissue engineering of oral mucosa, genomic & genetic markers

Services : BioanalyzerPerson in charge: Pn. Junaida-Science Officer, Tel no. 03-79676461

LABORATORY RESEARCH

Services/Facilities – CMBRLCENTRIFUGE 

ICE MAKER

LAMINAR FLOW  

AUTOCLAVE

 NANODROP FAST REAL TIME PCR SYSTEM

 THERMAL CYCLER AUTOMATED DNA EXTRACTOR

 BIOANALYZER ODYSSEY IMAGING SYSTEM

 GEL IMAGING SYSTEMCO2 INCUBATOR

 CONCENTRATOR MICROPLATE READER (TECAN INFINITE)

  LIQUID NITROGEN TANK

LABORATORY RESEARCH

• LAB INTRODUCTIONBUARL – BALAI UNGKU AZIZ RESEARCH LAB (Balai Ungku Aziz, Faculty of Dentistry)Research focus area:Oral microbiology, oral biochemistry, oral histology &

molecular microbiologyExperiments in proteomics, tissue culture & plant-

based productsPerson in charge: En. Rafiki-MLT, Tel. no 03-79677450

LABORATORY RESEARCH

Services/Facilities - BUARLHPLC BIOLOGY

ELISA READER FREEZE DRYER

SPECTROPHOTOMETER DIAMOND CUTTER

PHENOM SEM 2 D ELCTROPHORESIS

REFRIGERATED CENTRIFUGE  

LABORATORY RESEARCH

• LAB INTRODUCTIONOPDRL – ORAL PHATOLOGY DIAGNOSTIC &

RESEARCH LAB (Level 1, Block B of the Main Faculty Building)

Provide diagnostic pathological services to hospitals, clinics & referring organizations.

Services : Routine paraffin sectioning & staining, cryosectioning, usage of precision cutting system for detailed analysis of hard tissue structures, immunohistochemistry & direct immunoflourescence

Preparing teaching & provides a conducive environment for postgraduate students pursuing research

Person in charge: Pn. Rusnani-MLT, Tel. no 03-79674815/4569

LABORATORY RESEARCH

Services/Facilities - OPDRLCRYOSTAT MICROTOMY

SERVICE IMMUNOHISTOCHEMISTRY

PROCESSING OF SAMPLE  + EMBEDDING

IMAGE ANALYZER WORKSTATION

PRECISION CUTTING SYSTEM

WATER PURIFICATION SYSTEM

H & E STAIN   PAPANICOLAOU STAINSPECIAL STAIN

LABORATORY RESEARCH

• LAB INTRODUCTIONRDL – RESEARCH DIAGNOSTIC LAB

(Level 6, Postgraduate & Research Tower)

Provide diagnostic pathological services for researchers

Services : Routine paraffin sectioning & staining, cryosectioning, slide scanning, Laser Capture Microdissection, thin prep tissue processing and oral advance tissue processing

Person in charge: Pn. Nurul/ Cik Lini – Pen. Pegawai Sains, Tel. no 03-79676499/6462

LABORATORY RESEARCH

Services/Facilities - RDLMICROTOME CRYOSTAT

CYTOSPIN (ORAL ADVANCED SAMPLE PROCESSING)

H&E STAINING

LASER CAPTURE MICRODISSECTION (LCM)

THIN PREP PROCESSOR

AUTOSCANER – SLIDE SCANNER

CLEARCYTE SYSTEM

GENERAL LAB SAFETY RULES

LABORATORY RESEARCH

• General Safety Rules

RA’s are not permitted to enter any laboratory, workshop and store area without permission.

RA’s are only permitted in laboratories for official research or during practical classes.

Coats, bags and other personal belongings must NOT be taken into laboratories

Lab coats should not be worn outside the laboratory or workshop. At the end of a research, all electronic and electrical equipment should be

switched off (unless otherwise instructed). All hazardous materials or apparatus must be rendered safe, or disposed off,

in the manner indicated by technical staff or by established procedures before leaving the laboratory.

Eating, drinking and storing of food are strictly prohibited inside any laboratory

LABORATORY RESEARCH

• Access

Fire doors must be closed at all times except for the fire doors that are designed to be open under normal conditions .

All RA’s are given access card to enter the laboratory. Do not give your access card to other RA’s as we are all monitored by a CCTV.

Carpet or electrical cords are not allowed to run under (through) the fire doors.

All laboratory door exits, access to emergency equipment and passage ways must be kept clear of obstructions.

LABORATORY RESEARCH

• Housekeeping

At the end of the workday in any laboratory all work areas, benches and sinks must be left in a clean and tidy condition. It is the user’s responsibility to clear up any mess after using the laboratory.

Stacked materials should be in stable condition. No bottles, plastic containers or experiment materials are allowed to be

kept under the benches, on the floor Laboratory benches should not be used as general storage area. Apparatus, glassware and/or chemicals not in use should be kept in proper storage area or in your designated cabinets.

Disposal of waste should be done safely and promptly using the designated bins/containers.

All radioactive, chemical and biological materials must be properly labelled and stored inside its designated cabinets. .

All chemical bottles or containers must be capped with a proper cap or seal except when being used

LABORATORY RESEARCH

• General Workshop Behavior All users of laboratory should follow all instructions given by the staff /

technician in charge. Do not use any equipment without proper training, usage instruction and

authorization. Alternately, equipment may be used in the presence of a lecturer, supervisor or technician in charge. Do not conduct unauthorized experiments, activities or tasks in any of the workshops.

Wear the lab coats, gloves, goggles, covered shoes when you are in the laboratory.

RA’s must dressed appropriately in laboratory. Head scarves must be tucked in (under the clothes, lab coat or overall) and long hair should be tied back.

Do not work on your own (especially at night/after office hours) as in the event of an emergency, there is no one around who is able to provide help.

LABORATORY RESEARCH

• Equipments/Machine

Before operating any machine tools, be sure to know the method to operate its emergency stop device. Ask for assistance from the technician / staff in charge if unsure.

Always give your full concentration to the job when operating a machine. Never leave the machine unattended, even if it is only for a few seconds or a few meters away or even overnight.

Before operating any machine, make sure it is in good order and properly adjusted and lubricated. If not, report it to your supervisor or technician and do not use the machine.

Before cleaning a machine, stop the machine and remove the plug to stop electrical supply (isolate it electrically). Machine cleaning should be done with proper training and direct supervision from the technician / staff in charge. Report the matter to your supervisor or technician in charge when the machine needs maintenance or repair.

• Personal Protective Equipment (PPE) and Equipment

• Laboratory Coats RA’s must wear appropriate laboratory coats in labs at all times. RA’s not in possession of a

laboratory coat will NOT be allowed to work in these laboratories. Laboratory coats are not to be worn outside of the laboratories, especially in public areas such as canteen, tea room, staff rest area, reading rooms to avoid cross contamination.

• Eye Protection All persons must wear approved eye protection (safety glasses) at all times in the chemical

laboratories. • Gloves Gloves must be worn when handling chemicals or any hazardous materials. Be careful not to

contaminate switches, handles, plugs and other equipment, door knobs, telephones when wearing protective gloves.

• Footwear All persons must wear proper closed-top shoes in all laboratories and workshops. Sandals and

slippers are not allowed. • Masks/Respirators Suitable masks / respirators must be worn whenever a process involving volatile chemicals or

machine churn out dust particles.

LABORATORY RESEARCH

LABORATORY RESEARCH

• Conclusion All standard operating procedures and safe practices must be

followed at all times. Each laboratory usually has their own safety rules depending to the type of research activities that are being carried out. Exercising caution while working in laboratories will reduce accidents and injuries.

LABORATORY RESEARCH

• RESEARCH LAB FORMAPPLICANT CONTACT INFORMATIONSAMPLE INFORMATIONAPPROVAL BY SUPERVISOR/ PRINCIPAL

INVESTIGATORCOMMON ITEMEQUIPMENTSERVICES