RESULTS OF ECOSYSTEMS PLUS® INJECTION IN SECONDARY AERATION

LAGOONS

GLOBAL FOOD MANUFACTURER

(SEPTEMBER 6-22 2006)

PRIMARY PROJECT OBJECTIVES: •  Demonstrate ESP®'s ability to increase the transfer of

dissolved oxygen into the secondary aeration lagoons.

•  A baseline of DO measurements was established for the

period 8/17-23/06 in both lagoons 2&3.

•  The levels were measured daily at 5 separate points along

the length of each lagoon.The points were:

o  #1-Influent distribution box.

o  #2-The beginning of lagoon.

o  #3-Midway point. (Opposite surface aerator #1).

o  #4-End of the lagoon. (Opposite surface aerator #2).

o  #5-Effluent discharge box.

Clarifier 1

Flow Equalization Tanks With

Mixers

Aeration Blowers

Sludge Storage

Polymer Injection

Anaerobic Bio- Reactors

To sludge belt press and land

application

Bio-Organic Catalyst Injection Point

Gas Flare

Aeration Lagoons

Clarifier 2

Influent Wet Well (.75 MGD) Food

Manufacturing Discharge

Bio-Gas Boiler

RAS

To Chlorine Contact and Discharge

Activated Sludge

WAS

EcoSystem Plus

GLOBAL FOOD MANUFACTURER- WWTP ESP INJECTION POINT

Effluent 24 hr-7 Day Composite Sample Point

Clarifier Sample Point

DO Sample Points #5-Discharge #4 #3 #2

#1

Flow Direction

ESP INJECTION POINT INTO LAGOON # 3

Aeration Building

Injection Point

Flow Equalization Tanks

SECONDARY AERATION LAGOON # 3 WITH FINE BUBBLE DIFFUSERS AND SURFACE

AERATORS

Secondary Clarifiers Anaerobic Bio- Reactor Building

Flow Direction

TECHNOLOGY BREAKTHROUGH GREATER AERATION EFFICIENCY:

•  Improves oxygen transfer and uptake.

•  Reduces energy use, while increasing DO.

•  Increases biological activity and biomass.

•  Increases treatment plant capacity.

•  Reduces volatile organics for odor control.

•  Improves nitrification and de-nitrification

cycle.

TECHNOLOGY BREAKTHROUGH SOLUBILIZATION OF LIPIDS:

•  Solubilization of fats, oils, and greases (FOG’s).

•  This is an accelerated natural catalytic process in

which lipid ester bonds are rapidly cleaved,

reducing their molecular structure to both glycerol

and fatty acids.

•  Glycerol and fatty acids are then readily available

to wastewater micro-organisms as a high-energy

food source of carbon for enhancement of

nitrification and de-nitrification reduction processes.

STUDY PARAMETERS: (DISSOLVED OXYGEN TESTING)

•  Since the RAS could not be separated, it was

not possible to isolate Lagoon #3 and #2.

•  Increases in DO were examined and compared

as a combined total for lagoons #2 and #3.

•  The ON and OFF time periods are both

compared against the baseline.

SECONDARY CHANNEL FLOWS:

•  The average daily influent flow from the

anaerobic bio-reactor is .75 MGD, and this flow

is split into two (2) influent flows of .365 MGD

to the aerobic lagoons #2 and #3.

•  These secondary aeration lagoons have a

volume of 1.5 million gallons each.

•  Each aeration lagoon has two (2) surface

aerators, along with six 6 sub-surface, fine-

bubble, air diffusers.

SECONDARY CHANNELS LOADING:

•  Average return activated sludge (RAS) flow,

per-lagoon, is .144 MGD.

•  Baseline average daily loading was 10,677 lbs.

TCOD.

•  Average daily loading during study

(9/6-22/06) was 14,818 lbs. TCOD.

STUDY START-UP:

•  EcoSystems Plus ® was injected into the #3 lagoon

influent distribution box: 9/6-22/06 @ 3.6 GPD.

•  The results of increased DO levels (9/6-22/06) are

compared against various baselines.

STUDY TESTING: •  NH3-N and TCOD levels, sampled at the

clarifier discharge point, were compared

against historic baselines.

•  Final sludge volumes from dewatering were

compared against a baseline.

•  PO4-P Removal Rates were compared as well.

RESULTS IN SECONDARY AERATION LAGOONS: (DISSOLVED OXYGEN)

•  45% increase in DO measured at the lagoon

discharge point: study period 9/6-22/06,

against baseline period 8/17-23/06.

§  Surface aerators both on and off.

§  Concurrent with 39% increase in TCOD

loading, over baselines.

RESULTS IN SECONDARY AERATION LAGOONS: (DISSOLVED OXYGEN)

•  70% increase in DO measured at the lagoon

discharge point with ESP injection, against the

baseline period.

•  Surface aerators continually on.

•  Concurrent with 39% increase in TCOD

loading.

COMPARISON OF TOTAL (DO) INCREASES IN SECONDARY AERATORS:

10,677

14,818

0.490.51

0.89

0.75

0.26

0.69

0.83

0.71

1.10

0.35

9,000

10,000

11,000

12,000

13,000

14,000

15,000

16,000

Combined Lagoon 2&3Header Box

Combined -Stage 1 Combined -Stage 2 Combined -Stage 3 Combined-DischargeBox

Lb

s O

f T

CO

D L

OA

DIN

G

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

1.1

1.2

DO

mg

/L

AVER Lagoon Loading-Lbs of TCOD-8/17-23/06 AVER Lagoon Loading-Lbs of TCOD-9/6-22/06

Baseline DO Levels-8/17-23/06 Combined With ESP Inj.-9/6-22/06

DO Increased 45 % Over Baseline,In spite of 39% Increase in Lbs of TCOD

0.35

0.490.51

0.89

0.75

0.27

0.77

1.12

1.01

1.28

0.2

0.4

0.6

0.8

1

1.2

1.4

Com bine d Lagoon 2&3 He ade rB ox

Com bine d -Stage 1 Com bine d -Stage 2 Com bine d -Stage 3 Com bine d-Discharge B ox

DO

mg/

L

Baseline DO Levels -8/17-23/06 Aver -9/6-14 All Aerators On

DO Increased 70 % Over baseline

COMPARISON OF (DO) INCREASES WITH ALL SURFACE AERATORS ON:

SECONDARY AERATION LAGOONS: (NH3, TKN & TCOD REDUCTIONS)

•  69.3 % reduction in NH3 & TKN- (mg/l) @

clarifier discharge.

•  36.2% reduction in TCOD -(mg/l) @ clarifier

discharge.

1.95

6.35

0

0.5

1

1.5

2

2.5

3

3.5

4

4.5

5

5.5

6

6.5

7

7.5

8

8.5

9

9/6

/06

9/7

/06

9/8

/06

9/9

/06

9/1

0/0

6

9/1

1/0

6

9/1

2/0

6

9/1

3/0

6

9/1

4/0

6

9/1

5/0

6

9/1

6/0

6

9/1

7/0

6

9/1

8/0

6

9/1

9/0

6

9/2

0/0

6

9/2

1/0

6

9/2

2/0

6

ES

P A

VE

R I

NJ

.P

ER

IOD

9/2

3/0

6

9/2

4/0

6

9/2

5/0

6

9/2

6/0

6

9/2

7/0

6

9/2

8/0

6

9/2

9/0

6

9/3

0/0

6

NH

3-N

mg

/L

ESP Inj Starts-9/6 @3:30 pm

ESP Inj Ends-9/22

NH3-N AVER With ESP Inj. Re duce d 69.3 %

REDUCTIONS OF NH3 - N AT CLARIFIER:

37

58

0

10

20

30

40

50

60

70

800

9/0

6/0

6

09

/07

/06

09

/08

/06

09

/09

/06

09

/10

/06

09

/11

/06

09

/12

/06

09

/13

/06

09

/14

/06

09

/15

/06

09

/16

/06

09

/17

/06

09

/18

/06

09

/19

/06

09

/20

/06

09

/21

/06

09

/22

/06

ES

P A

VE

R I

NJ

.P

ER

IOD

09

/23

/06

09

/24

/06

09

/25

/06

09

/26

/06

09

/27

/06

09

/28

/06

09

/29

/06

TC

OD

mg/L

ESP Inj. Starts 9/6

ESP Inj. Ends 9/22

Aver TCOD Reduction Of 36.2% With ESP INJECTION

REDUCTIONS IN TCOD AT CLARIFIER:

SECONDARY AERATION LAGOONS (TKN, NH3-N & NITRATES)

REDUCTIONS IN FINAL DISCHARGE LEVELS

•  68.6% in TKN mg/l @ Effluent discharge

•  85.7% in Ammonia as NH-3 mg/l @ Effluent

discharge

•  83.3 % in Nitrate as N mg/l @ Effluent

discharge

4.8

3.8

1.6 1.51.4

1.80

0.40

0.10

0.8

0.100.3 0.26

0.53

0.050.3 0.39

5.1

3.4

0.0

0.5

1.0

1.5

2.0

2.5

3.0

3.5

4.0

4.5

5.0

5.5

60 day Baseline Aver 9/6 9/13 9/20 Aver-With ESP Inj. 10/4

mg/

L

24 hr. comp. TKN mg/l Ammonia as N mg/l nitrate as N mg/l

Continued "Carry Over Effect" Of ESP

REDUCTION WITH ESP INJECTION68.6% in TKN85.7% in Ammonia as N83.3 % in Nitrate as N

IMPROVED FINAL EFFLUENT DISCHARGES

IMPROVEMENTS IN PO4-P DISCHARGES:

•  Baseline PO4-P % removal was 89.6 %

•  Removal rates improved to 97.3 % for three

weeks.

•  One week (9/20/06) only improved by .3 %,

however the influent load increased by 14%

during that week.

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89.6 89.3

97.2 97.5

93.4

97.4

89.9

88.0 89.0 90.0 91.0 92.0 93.0 94.0 95.0 96.0 97.0 98.0

60 day-Baseline Aver 9/6/2006 9/13/2006 9/20/2006 9/27/2006 Aver With ESP Inj 10/4/2006

% R

emov

al o

f Inf

luen

t vs.

Eff

luen

t Dis

char

ge P

O4-

P

.3 % Increase in % removal rate, In spite of a 14% increase of Influent Loading

8 % Increase In Removal Rate with ESP " Carry Over Effect"

IMPROVED REMOVAL OF PO4-P DISCHARGES: ( INFLUENT VS EFFLUENT)

REDUCTIONS IN SLUDGE VOLUMES: Ø 28.3 % SLUDGE REDUCTION

•  Baseline level of sludge pressed:daily

average of 57,065 Lbs.

•  Study period sludge pressed: daily average

of 40,892 Lbs.

•  Concurrent during study period there was a

39% increase in Lbs. of TCOD influent

loading.