Retrieving Data GuideRetrieving Data Guide

Rebecca and John Moores UCSD Cancer Center DNA Rebecca and John Moores UCSD Cancer Center DNA Sequencing Shared ResourceSequencing Shared Resource

Retrieving DataRetrieving Data

Go to dnaLIMS website Go to dnaLIMS website http://http://dnalimsdnalims..ucsducsd..eduedu//

Click “Login to Click “Login to dnaLIMS” and enter dnaLIMS” and enter Login Name and Login Name and PasswordPassword

Click on the Click on the “Retrieve “Retrieve Sequencing Sequencing Results” linkResults” link

Select the Select the Sample Sample Sheet Sheet Number Number and click and click “Submit”“Submit”

Samples can be Samples can be viewed as text viewed as text and and chromatogram chromatogram files individually files individually or downloaded or downloaded together in a together in a compressed compressed format.format.

Viewing Individual Text and Viewing Individual Text and Chromatogram FilesChromatogram Files

Click the “View” button Click the “View” button next to the file you wish next to the file you wish to viewto view

The chromatogram file The chromatogram file will automatically will automatically display in the browser display in the browser window (no software window (no software required) required)

To view the text To view the text sequence click either sequence click either the “Initial Sequence” or the “Initial Sequence” or “Phred Sequence” “Phred Sequence” buttonbutton

Downloading FilesDownloading Files

With a PC and With a PC and Internet Explorer 5Internet Explorer 5 Click here to get WinZip, Click here to get WinZip,

which is needed to which is needed to uncompress if you uncompress if you download all trace and download all trace and chromatograms files (for chromatograms files (for all users)all users)

Users must have Users must have Chromas software Chromas software installed on their installed on their computer to view computer to view downloaded filesdownloaded files

For Macs For Macs OS9+OS9+ Clients will need Stuffit Clients will need Stuffit

Expander to extract all Expander to extract all trace files and trace files and chromatogramschromatograms

Mac Resource Fork Repair Mac Resource Fork Repair Tool must be downloaded Tool must be downloaded (this can be found on the (this can be found on the Support Tools link on the Support Tools link on the client menu page of client menu page of dnaLIMS)dnaLIMS)

This tool will extract itself This tool will extract itself automatically into 2 filesautomatically into 2 files

Delete the “.hqx” file and put the Delete the “.hqx” file and put the other on the desktopother on the desktop

To view the trace files and To view the trace files and chromatograms, click the link to chromatograms, click the link to download themdownload them

Once downloaded it may Once downloaded it may extracted automatically, if not extracted automatically, if not use Stuffit Expander to extract use Stuffit Expander to extract filesfiles

Drag folder containing the Drag folder containing the extracted files onto the “Mac extracted files onto the “Mac Resource Fork Repair Tool” on the Resource Fork Repair Tool” on the desktop and click “OK” at the desktop and click “OK” at the promptprompt

Open the folder and view the filesOpen the folder and view the files You must have EditView software You must have EditView software

installed on your computer to installed on your computer to view chromatogram filesview chromatogram files

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