rosalin technique

Rosalin Technique

By: Abby John

http://www.offthemark.com/cartoons/clone

http://www.offthemark.com/cartoons/clone

What is the Rosalin Technique?

• The Rosalin Technique is a type of cloning • The Rosalin Technique is a variation of somatic

cell nuclear transfer that was developed by researchers at the Rosalin Institute

• A somatic cell nuclear transfer is a laboratory technique to create a clone embryo with a donor nucleus

TECHNIQUE

ROSALIN

What has it been used for?

• It has been used to create Dolly • Dolly was a sheep and the first mammal to be

cloned by the Rosalin Technique• She has been called “the world’s most famous

sheep”

Dolly!

How They Created Dolly

• The Researchers first took mammary gland cells from a Finn Dorset Sheep

• They then took those cells and grew them in tissue culture

• The tissue culture lets the scientists change the characteristics of the cell

• They made sure that the cell didn’t have the nutrients it needed so it didn’t grow and divide

How They Created Dolly Cont.

• Researchers then took the nucleus out of an unfertilized egg from a Scottish Blackface ewe

• They injected the nucleus from the original mammary cell into the unfertilized egg

Egg Cell

http://www.shutterstock.com/pic-14214475/stock-photo-human-egg-cell.html




• They used a small spark of electricity to fuse the nucleus to the cytoplasm

• The electricity also helps the cells to become more likely to divide

http://www.bikanertimes.com/1-died-due-to-electric-shock/




• The new cell was then put into a Blackface Ewe• The whole procedure was repeated 276 times • After 148 days Dolly was born!

http://www.stackyard.com/news/2009/03/sheep/01_blackface_show.html



Dolly Facts

• Before the researchers at the Rosalin Institute created Dolly no animal had ever been cloned using an adult egg

• Dolly was born on July 5, 1996 and died on February 14, 2003

• She died from a lung disease

http://www.nature.com/scitable/topicpage/gene-expression-regulates-cell-differentiation-931

http://www.nextnature.net/2008/01/fake-for-real-dolly-the-cloned-sheep/






http://www.martinfrost.ws/htmlfiles/roslin_dolly.html



http://www.rpi.edu/dept/chem-eng/Biotech-Environ/cloning/bioTech1.html



Ethics

• The Rosalin Technique is a very controversial subject

• Some scientists think that it is a good thing, because it helps with the advancement in science

• Some people think it is not ethical and is “playing god”

http://eyegillian.wordpress.com/2008/03/09/is-cloning-the-wave-of-the-future/



http://www.britannica.com/media/full/60421

http://www.britannica.com/media/full/60421

rosalin technique

Technology