rr322306 protein engineering

Code No: RR322306 Set No. 1

III B.Tech II Semester Supplementary Examinations, Aug/Sep 2006PROTEIN ENGINEERING

(Bio-Technology)Time: 3 hours Max Marks: 80

Answer any FIVE QuestionsAll Questions carry equal marks

1. (a) What is a peptide bond?

(b) Describe in detail how peptide bonds are formed. [8+8]

2. Describe the role of hydrogen bonds in tertiary structure of protein. [16]

3. Justify the statement - Information of final structure of protein lies in its primarystructure. [16]

4. Describe the activity of DNA polymerases and explain their action in detail. [16]

5. (a) What are proteases.

(b) Describe different classes of proteases with examples.

(c) Explain the action of serine proteases in details. [5+6+5]

6. Define antigenic determinants? Explain major categories of Antigenic determinanton Immunoglobulins? [16]

7. Write about Pattern databases for storing information about protein families andmotifs? [16]

8. Explain the importance of protein designing in the field of Drug designing. [16]

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1. (a) Classify amino acids based on their chemical nature

(b) Describe any four general properties of amino acids. [4+12]

2. Explain briefly the methods employed in determining 3 D-structure of a Protein?[16]

3. Write short notes on:

(a) Glycosylation

(b) Sulfation [8+8]

4. Describe the zinc finger and their function. [16]




6. Explain the make up of photosynthetic center and their action with respect to theirstructure. [16]

7. Name a few Protein resource databases. Explain them in brief. What are thespecialities of these databases? [16]

8. (a) What do you mean by protein design?

(b) What are the goals of protein design? [8+8]

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1. What is Secondary Structure of protein? Discuss in details. [16]


(a) Fibrous proteins

(b) Globular proteins [8+8]

3. Aminoacid sequence of final protein is not same as dictated by Gene SequenceJustify above statement? [16]

4. Describe the action of restriction enzyme in detail. [16]




6. Explain the make up of photosynthetic center and their action with respect to theirstructure. [16]

7. Describe the following

(a) Scop Structural classification of proteins

(b) CATH Class, Architecture, Topology and Homology data bases. [8+8]

8. (a) What is Levinthal paradox? Discuss in detail.

(b) Describe the problems in protein folding in detail. [8+8]

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1. Write an essay on regular confirmation of Poly peptides? [16]

2. Describe the role of hydrogen bonds in tertiary structure of protein. [16]

3. Explain the concept of Levinthals Paradox in Protein Folding? [16]


(a) Zinc fingers

(b) Leucine zippers [8+8]

5. Describe the properties of three classes of Membrane proteins, how they differ fromone other and how they vary among themselves? [16]

6. Explain the production of Insulin by r DNA technology? [16]

7. Describe Oligonucleotide directed mutagenesis in protein engineering. [16]

8. Describe the basic principles with respect to protein design. [16]

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rr322306 protein engineering

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