Samba PreclinSamba 200

Product Presentation

Samba Preclin 420/360 Transducer

Ultra-miniature fiber optic pressure sensor –no bigger than a grain of salt on a hair!Highly sophisticated, accurate, fast and sensitive instrument designed to measure pressure in gas or liquid.

Constructed with patented state-of-the-art micro mechanic and fiber optic technology.

A first-class tool for building knowledge and solving problems in numerous application areas.

Samba Preclin moves science into new territories!

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Samba Sensors’ Technology

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The Sensor

Optical fiber∅ 0.25mm

SensorLow pressure ∅ 0.42mmHigh pressure ∅ 0.36mm

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Measuring Hydrostatic Pressure

Hydrostatic pressure

Space filled withGAS or LIQUID

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The Sensor on a Finger Tip

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X-ray Visibility

Radiopaque coating∅ 0.40mm

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Samba Products

Main Products

Control units with analog and serial ports. Samba Data Collection software for PC.

For research use.

Ultra-miniature optical pressure transducers in different versions.For preclinical and industrial use.

Not FDA approved

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Preclinical Transducers Samba Preclin 420 LP

• Miniature optical pressure transducers for research in vitro and in vivo. Low pressure range (-50 to +350 mbar). Also with radiopaque coating. 4m long.

Samba Preclin 420/360 MR• Miniature optical pressure transducers

for research in vitro and in vivo. Extra long for use in MRI facilities. Low and high pressure ranges. 10m long.

Samba Preclin 360 HP• Miniature optical pressure transducers

for research in vitro and in vivo. Three high pressure ranges (-0.1 to 5 bar, -0.1 to 10 bar, -0.1 to 17 bar). Also with radiopaque coating. 4m long.

Protective sensor covers are available for special applications

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Samba Preclin Transducer Pressure can be measured in very small cavities or directly

in the tissue of small (or large) animals. The ultra-miniature size minimizes insertion trauma. Insensitive to EM radiation and RF fields (MRI scanner,

microwaves etc). Direct measurements with very high precision are made at

the sensor location. The transducer does not affect the measuring environment

itself. Low risk – no electricity or bio-hazardous material. Long transducer possible (up to 10m).

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Samba 201 Control Unit One fiber-optic port Battery operation Internal memory Absolute or relative measurement Internal barometer 3.2 inch display USB 2.0, analog and RS-232 ports Analogue output: 0-5V Sampling frequency: 1 Hz – 40 kHz Resolution: 0.1 mbar (~1mm H2O) Numerical resolution: 15 bit Numerical res.: 0.1 mbar Operating temp.: 15 – 35 C Size: 215x110x45mm

(8.5x4.3x1.8 in) Weight 950g (2.1 lbs)

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Samba 202 Control Unit Two fiber-optic ports Battery operation Internal memory Absolute or relative measurement Internal barometer 3.2 inch display USB 2.0, analog and RS-232 ports Analogue output: 0-5V Sampling frequency: 1 Hz – 40 kHz Resolution: 0.1 mbar (~1mm H2O) Numerical resolution: 15 bit Numerical res.: 0.1 mbar Operating temp.: 15 – 35°C Size: 215x110x45mm

(8.5x4.3x1.8 in) Weight 950g (2.1 lbs)

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Fast and Simple Start-Up Procedure1. Insert the Samba transducer

The transducer is factory calibrated - No further calibration is needed!2. Turn on your Samba 200 Control Unit

Allow internal circuitry temperature to stabilize (1-2 minutes)3. Set the “zero baseline” against ambient air pressure

The transducer is factory calibrated - No further calibration is needed! Simply follow instructions on the screen (‘ZERO’ or ‘PREVIOUS’)

4. If needed set measurement rate, Absolute/Relative mode, pressure unit etc. or load pre-defined Profile

5. Connect to PC or Data Acquisition System (DAS)When connected calibrate DAS by pressing ‘ANALOG CALIB.’

6. Remove protective cover, apply sensor in object and start measuring!

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Samba vs. CompetitionThe Samba system…1. …has a simple and fast start-up procedure (~3 min) – just Plug’n Play!

2. …is factory calibrated and doesn’t need further calibration, only an automatic “zero baseline” set against the atmospheric pressure.

3. …is extremely fast (up to 40 kHz data sampling rate) and can record high-speed trauma events, for example ICP events caused by collisions.

4. …is fully MRI compatible and completely insensitive to any electro-magnetic waves radio waves or electrical noise. Real-time measurements and simultaneous recording of both visual and physiological effects of for example a drug is possible. Gating (MRI scanner triggering) for improved MR image quality and movie sequences is also feasible.

5. …can measure pressure both in gas and liquid, which allows a very broad use.

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Examples ofApplications

Inserting Transducer into a Mouse Heart

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Monitoring Pressure in a Mouse Heart

Transducer insertion

Am J Physiol Heart Circ Physiol, Vol. 285, pp. H2233-H2239, 2003

Right Ventricular Pressurealso possible!

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Pulse Wave Analysis (PWA)• PWA, i.e. the analysis of the pulse

pressure wave, is a method for studying vascular stiffness

• PWA provides valuable information for the characterization of cardiovascular disease, atherosclerosis and endothelial function

• The analysis is preferably made on a number of pulse wave cycles selected from a specific time period

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Parameters in Samba PWA Software• Pdiast = end-diastolic pressure

• Psyst = inflection point in systole

• Paug = max pressure during entire cycle

• RRint = heart cycle length

• Tsyst = time between pdiast and psyst

• PP = paug – pdiast

• Taix = time from pdiast to paug

• Aug = Paug – Psyst

• AIx (augmentation index) = Aug/(Paug-Pdiast)

• Further parameters related to the Dicrotic Notch will be available in future software version

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Transducer Introduction into Rat Tail Artery

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Triggering the MR Scanner (Gating)

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100.00

0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00

Time [s]

Pres

sure

[mm

Hg]

Sharper MR images& movie sequences!

Triggering point: Exhalation & min. diastolic blood pressure

B. Bjelke, MD, PhD, Karolinska Institute, Sweden 2003

Respiration

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Gating on Samba Signal (IBP) Image of rat heart MRI scanner triggered

on rat tail IBP signal Advantages

• Improved image quality(less artifacts than ECG)

• Real-time IBP monitoring

• Volume & CO• Movie sequences

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MRI Movie Sequence

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Intracranial Monitoring on a Rat

Samba transducer

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ICP in Fluid Percussion Model

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500

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2000

2500

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3500

0,00 0,10 0,20

Time [s]

Intracranial PressureWater Pressure

Pres

sure

[mba

r]

Acta Neurochir Vol. 147, No. 7, pp. 775-780, 2005

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Rabbit CNS Trauma Model

Eur J Neurosci. Vol. 21, No. 10, pp. 2876-82, 2005

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Rabbit CNS Trauma Model

0.5

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1

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0.08 0.085 0.09 0.095 0.1 0.105 0.11

Time (s)

-220000

-170000

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-70000

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30000

Rot

. acc

.(rad

/s^2

)

Occipital lobe pressure transducer Parietotemporal lobe pressure transducer Rotational acceleration

Pres

sure

[bar

]

Eur J Neurosci. Vol. 21, No. 10, pp. 2876-82, 2005

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Spinal Disc Measurements

Samba transducer insertedinto disc via a cannula

Proceedings SPIE 3570, 1998

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Proceedings SPIE 3570, 1998

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Urodynamic InvestigationTransurethral introduction of transducer into bladder

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Urodynamic InvestigationTransurethral introduction of transducer into bladder

Courtesy of Prof. Ulla Sillén, Dept. of Pediatrics Sahlgrenska University Hospital, Sweden

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Airway Pressure Monitoring

Endotracheal tube

Ventilator pressure sensor

Samba sensor

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Pressure Measurement in Pediatric ETTTransducer inserted through tube into lower trachea

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5

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25

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1 51 101 151 201 251 301Samples [25 Hz]

SambaRespiratory

Rel

ativ

e pr

essu

re [c

mH

2O]

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Intra-folliclular Pressure(during open surgery)

Time [sec]

P Dahm-Kähler et al. Poster Läkarstämman, 2004

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Clinical Samba Projects1. Urodynamic investigations2. Respiratory pressures3. Intracranial pressures4. Intervertebral disc pressure (spinal surgery)5. Cardiovascular blood pressures6. Gastro-intestinal pressures7. Intra-follicle pressure (gynecology)

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Samba’s unique offering

High-precision pressure measurements can beperformed where never before possible.

New science and knowledge can be revealedleading to more publications.

APPLICATION AREAS:- Heart and Circulation- Brain and Nervous System- Lungs and Breathing- Gastro-intestinal System- Kidney and Urinary System- Reproduction System- Muscles- Diseases like Cancers, Stroke, Spinal Disorders and more

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ContactHarvard Apparatus, Ltd

Fircroft Way, Edenbridge

Kent TN8 6HEEngland

United KingdomPhone: (44) 1732-864001

Fax: (44) 1732-863356E-Mail: sales@harvardapparatus.co.ukInternet: www.harvardapparatus.co.uk

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