sanita-kun r user friendly microbial detection !
TRANSCRIPT
Sanita-kun Sanita-kun RR
=The ready-to-use cultural medium sheet for the microbial detection =
User friendly microbial detection !
Chisso CorporationChisso Corporation e-mail [email protected] [email protected] web http web http://://www.chissowww.chisso.co..co.jpjp//sanitasanita//
Chisso Corporation
What is the SANITAKUN?
SANITAKUN is our original product Sheet Medium.
SANITAKUN is a ready-to use cultural medium sheet equipped with non-woven fabric.
SANITAKUN provides easy and accurate detection of microorganisms to customers as an alternative to agar plate.
The structure of SANITAKUN
Apply 1ml sample solution
incubate
Chisso Corporation
Transparentcover film
(oxygen permeability)
Non-woven fabric
Water-soluble high polymernutrientschromogeninhibitor
Adhesive film
Fabric pad
waterproof sheet
Applied sample solution diffuses throughout the entire pad in a few seconds.
High viscous solution dissolves, it pushes up microorganism to the fabric surface. The Microorganism grows and forms colony on the surface and it colors by chromogen.
Chisso Corporation
SANITAKUN line-ups
SANITAKUN Chromogenic principal incubation
Aerobic Count
Coliform
Yeast & Mold
Staph. aureus
Salmonella
Chromogen(TTC) colors red by a microbial metabolism.Chromogen colors blue or blue-green by
-galactosidase that coliform generates.
Chromogen colors blue or indigo blue byesterase that yeast or mold generates.
Chromogen colors black spot and blue halo around the spot.
Chromogen colors blue by -galactosidase that salmonella generates. (Chromogen dyes indigo blue by
-galactosidase that Coliform generates.)
35 48hr
35 24hr
25 48hr
35 24hr
35 24hr
E.coli/ColiformChromogen colors blue or blue-green by -galactosidase that coliform generates. Chromogen dyes indigo blue by
-glucuronidase that E.coli generates.35 24hr
Aerobic Count Coliform
Staph. aureus
Yeast & Mold
Salmonella
Chisso Corporation SANITAKUN chromogenic characteristic
E.coli/Coliform
Chisso Corporation
1) Easy to use2) Multi applications3) Accurate & Quantitative4) Reliability5) Ready-to-use & Time saving6) Space saving & Small volume of waste7) Long validity
User friendly Care free Reliable
Concept of SANITAKUN
Chisso Corporation
Characteristics 1:
Easy to use
Incubation
Foods or swabbing sample solution
Direct stamping
Characteristics 2 :
Multi applicationsChisso Corporation
Swabbing test used swabbing kit
Fall out test
Direct stamping
Water filtrate
Swabbing
Food sample test
Characteristics 3 :
Accurate & QuantitativeChisso Corporation
Incubation for 48h
y = 1.0338x - 0.2767
0
2
4
6
8
0 2 4 6 8
Plate Count Agar(logcfu/mL)
Sani
ta-k
un A
erob
icC
ount
(logc
fu/m
L)
SANITAKUN Aerobic Count
y = 0.9862x - 0.0197R2 = 0.992
1
2
3
4
5
6
7
8
1 2 3 4 5 6 7 8
Plate Count Agar (logcfu/g)
Sani
ta-k
un (l
ogcf
u/g)
Comparison between SANITAKUN Aerobic Count and agar plate / AOAC Official Method 966.23
Chisso Corporation SANITAKUN Aerobic count
0
2
4
6
8
0 2 4 6 8
0
2
4
6
8
0 2 4 6 8
y = 1.0194x - 0.2028
0
2
4
6
8
0 2 4 6 8
Daily dish(n=127)
0 2 4
Seaweed(n= 9)
y = 1.1297x - 0.5192
0
2
4
6
0 2 4 6
Egg(n=4)
y = 1.1242x - 0.4385
0
2
4
0 2 4
Bean(n=6)
y = 1.0044x + 0.0242
0
2
4
6
0 2 4 6
Candy (n=83) Dairy (n=5) Grain(n=16)
y = 1.0834x - 0.3872
0
2
4
6
8
0 2 4 6 8
y = 1.039x - 0.28730
2
4
6
8
0 2 4 6 8
Meat(n=29)
Fresh produce (n=52) Sea foods (n=77)Chisso Corporation
SANITAKUNAerobic Count(X;log agar plate,Y;log SANITAKUN)35 ,2days
Chisso Corporation
X-Gal agar(logcfu/g)Sa
nita
Kun
Col
iform
(log
cfu/
g)
Comparison between SANITAKUN and agar
SANITA-KUN Coliform
Desoxycholate agar(logcfu/g)
Sani
taK
unC
olifo
rm (l
ogcf
u/g)
Chisso Corporation
Comparison between SANITAKUN and PDagarSANITA-KUN Yeast & Mold (Rapid Type)
Characteristics 4 :ReliabilityChisso Corporation
The international certification
•SANITAKUN Aerobic Count, Coliform were approved by AOAC Research Institute as the Performance Tested Method.
•SANITAKUN E.Coli/Coliform was approved by AOAC Research Institute as the Performance Test Method on this July
•We will try to apply a validation such a AOAC PTM approval about SANITAKUN other item, then too.
The official recognition in Japan
SANITAKUN was appeared on the Food Sanitation Inspection Guide Line which official guide line of Japan.( Aerobic Count, Coliform, Staph. aureus, E.coli/Coliform )
Agar
SANITAKUN
Time
We can save 3 hours using SANITAKUN
incubation
autoclaving
cooling
Comparison of preparation time betweenSANITAKUN and agar plate (100 tests)
10 2 3 48 hours
Characteristics 5:
Ready-to-use & Time saving
StartSample application
measuring
Chisso Corporation
Start Sample application
10 agar plates
10 SANITAKUNs sheetsSpace saving
Small volume of waste
20 autoclaved plates 2.2litters
Characteristics 6:
Space saving Small volume of waste
to agar plates
to agar plates
400 autoclavedSANITAKUNs sheets
Chisso Corporation
Chisso Corporation
Characteristics 7:
Long validity
36Salmonella
24Staph. aureus
24Yeast & Mold
36Coliform
24Aerobic countvalidity (M)Sanita-kun
E.coli/Coliform 36
Validity from manufacturing date in a stock condition of tight sealingin a refrigerator.
Chisso Corporation
SANITA-KUN’s field
Food processingConvenience store’s venderHealthy foods
Poultry & Meat IndustriesFish IndustriesHotels & Restaurants
and OthersCosmeticWater supplyschool lunch etc