sc f5_practical student's module

7/28/2019 Sc f5_practical Student's Module

1/13

Name: _________________________________________ Class: ________

CHAPTER 1 (MICROORGANISM)

1. Effect of pH factor on the growth of microorganisms

Aim : ____________________________________________________________________________________________.

Problem statement : What is the effect of pH value on the growth of bacteria?

Hypothesis : ______________________________________________________________________________________Variable:

Manipulated : _____________________________________________.

Responding : _____________________________________________.

Constant : _____________________________________________.

Materials and Apparatus: 1. _____________________ 5. _______________________

2. _____________________ 6. _______________________

3. _____________________ 7. ______________________

4. _____________________

sterile

cotton

A B C

acidic pH 7 (neutral) Alkaline

Procedure:1. Three test tubes with nutrient broth are labelled A, B and C.

2. The solutions below are added to the test tubes:

A: 1 ml of dilute hydrochloric acid + 1 ml of bacterial culture

B: 1 ml of distilled water + 1 ml of bacterial culture

C: 1 ml of sodium hydroxide solution + 1 ml of bacterial culture

3. The test tubes are covered with a steril cotton.

4. All the three test tubes are kept in a cupboard.

5. After 4 days, observation on the growth of bacteria is recorded.

Tabulation of data:

Conclusion:

___________________________________________________________________________________

Prepared by Pn. Sity Salang 1

nutrient broth +

Bacteria culture

nutrient broth +

Bacteria culture

nutrient broth +

Bacteria culture

Sterile

cotton


2/13

Name: _________________________________________ Class: ________

2. Effect of temperature factor on the growth of microorganisms:

Aim :__________________________________________________________________________________________.

Problem statement : What is the effect of temperature on the growth of bacteria?

Hypothesis: ___________________________________________________________________________________.

Variable:

Manipulated: _____________________________________________

Responds : _____________________________________________

Constant : _____________________________________________

Materials and Apparatus: 1. _____________________ 5. _______________________

2. _____________________ 6. _______________________

3. _____________________ 7. ______________________

4. _____________________

A (50C ) B (370C ) C (600C )

Procedure:1. Three test tubes with nutrient broth are labelled A, B and C.

2. 1 ml of bacterial culture is poured onto the nutrient broth in each test tube.


4. The test tube are placed in various places as stated below:

A: kept in a refrigerator at temperature 50C

B: kept in an oven at temperature 370C

C: kept in an oven at temperature 800C


Tabulation of data:

Conclusion:


nutrient broth +Bacteria culture



Sterile cotton


3/13

Name: _________________________________________ Class: ________

_____________________________________________________________________________.

3. Effect of light on the growth of microorganisms:

Aim : __________________________________________________________________________.

Problem statement : What is the effect of light on the growth of bacteria?

Hypothesis: ____________________________________________________________________.

Variable:

Manipulated: ____________________________

Responds : ____________________________

Constant : ____________________________

Materials and Apparatus: 1. ____________________ 4. ____________________

2. ____________________ 5. ___________________

3. ____________________

sterile cotton

A (dark) B (under bright light)

Procedure:1. Two test tubes with nutrient broth are labelled A and B.2. 1 ml of bacterial culture is poured onto the nutrient broth in each test tube.


4. The test tubes are placed in various places as stated below:

A: is placed in a dark condition

B: is left under bright light.


Tabulation of data:

Conclusion:

____________________________________________________________________________.


nutrient broth +

Bacteria culture

nutrient broth +

Bacteria culture


4/13

Name: _________________________________________ Class: ________

4. Effect of humidity on the growth of microorganisms:

Aim : ____________________________________________________________________________.

Problem statement : What is the effect of humidity on the growth of bacteria?

Hypothesis : ______________________________________________________________________.

Variable:

Manipulated: ____________________________________

Responds : ____________________________________

Constant : ____________________________________

Materials and Apparatus: 1. _____________________ 4. ________________

2. _____________________ 5. _________________

3. ____________________ 6. _________________

sterile cotton

A (humid) B (dry)

Procedure:

1. Two test tubes are labelled A and B, and their contents are shown below.

A: Damp nutrient broth + 1 ml of bacterial culture

B: Dry nutrient broth + 1 ml of bacterial culture


3. All the three test tubes are kept in a cupboard.4. After 4 days, observation on the growth of bacteria is recorded.

Tabulation of data:

Conclusion:

_______________________________________________________________________________.


Damp nutrient broth +

Bacteria culture

Dry nutrient broth +

Bacteria culture


5/13

Name: _________________________________________ Class: ________

5. Effect of nutrient on the growth of microorganisms:

Aim : ____________________________________________________________________________

Problem statement : What is the effect of nutrient on the growth of bacteria?

Hypothesis : ______________________________________________________________________

Variable:

Manipulated: ______________________

Responds : ______________________

Constant : _______________________

Materials and Apparatus: 1. ____________________ 5. ___________________

2. ____________________ 4. ___________________

3. ____________________

sterile cotton

A B

(presence of nutrient) (without nutrient)

Procedure:

1. Two test tubes are labelled A and B, and their contents are shown below.

A: damp nutrient broth + 1 ml of bacterial culture

B: damp broth without nutrient + 1 ml of bacterial culture


3. All the three test tubes are kept in a cupboard.4. After 4 days, observation on the growth of bacteria is recorded.

Tabulation of data:

Conclusion:

____________________________________________________________________________.


Broth without nutrient +

Bacteria culture

Nutrient broth +

Bacteria culture


6/13

Name: _________________________________________ Class: ________

6. Effect of antibiotic on the growth of microorganisms:

Aim :____________________________________________________________________________.

Problem statement : What is the effect of antibiotic on the growth of bacteria?

Hypothesis : _____________________________________________________________________.

Variable:

Manipulated: _________________________________

Responds : _________________________________

Constant : __________________________________

Materials and Apparatus: 1. _______________________ 5. ______________________

2. ______________________ 6. ______________________

3. ______________________ 7. _____________________

4. ______________________

distil water nutrient agardisc + + penicillin disc

bacteria culture

A B

Procedure:

1. Two Petri dishes with nutrient agar are labelled A and B.

2. 1 ml of bacterial culture is poured onto the nutrient agar in each Petri dish.

3. A dish of filter paper that has been moistened with distil water is placed on the surface of

nutrient agar in Petri dish A as shown above.

4. A penicillin disc is placed on the surface of nutrient agar in Petri dish B.

5. All the two petri dishes are kept in a cupboard.


Tabulation of data:

Conclusion:



7/13

Name: _________________________________________ Class: ________

______________________________________________________________________________.

CAPTER 5 (MOTION)

A) INERTIA

Aim: ___________________________________________________________________________________.

Problem statement: What is the relationship between mass and inertia?

Hypothesis: _____________________________________________________________________________.

Variable:

Manipulated : _____________________________________________.

Responding : _____________________________________________.

Constant : _____________________________________________.

Materials and apparatus: 1. _______________________2. _______________________

3. _______________________

Procedure:1. Tin B is filled up with sand while tin A is left empty.

2. Both tins are swung at the same height.

3. The tin are stopped from swinging after a while.

4. The tin that is harder to swing and that is harder to stop are observed.

Tabulation Of Data:



8/13

Name: _________________________________________ Class: ________

Conclusion:

________________________________________________________________________________.

MOMENTUM

Relationship Between Mass And Momentum

Aim: ___________________________________________________________________________.

Problem statement: What is the relationship between mass and momentum?

Hypothesis: ______________________________________________________________________.

Variable:

Manipulated variable : ____________________________.

Responding variable : ____________________________

Constant variable : ____________________________

Materials and apparatus:

1. ________________________

2. ________________________3. ________________________

4. ________________________

(a) (b)

Procedure:

1. The apparatus as shown in diagram (a) is set up.2. A trolley is pushed gently to move down the track and collides a wooden block.

3. The displacement of wooden block is measured and recorded.

4. This experiment is repeated by using a two-stacked trolley as shown in diagram (b).

Tabulation Of Data:



9/13

Name: _________________________________________ Class: ________

Conclusion:

_____________________________________________________

2. Relationship Between Velocity And Momentum

Aim: ___________________________________________________________________________.Problem statement: What is the relationship between velocity and momentum?

Hypothesis: _____________________________________________________________________.

Variable:

Manipulated : ____________________________________________________

Responding : ____________________________________________________

Constant : _____________________________________________________

Materials and apparatus: 1. ________________________

2. ________________________

3. ________________________

4. ________________________

(a) (b)

Procedure:

1. The apparatus as shown in diagram (a) is set up.

2. A trolley is pushed gently down the track and it collides with a wooden block.

3. The displacement of wooden block is measured and recorded

4. This experiment is repeated by using two wooden blocks to make the track steeper as shown in

diagram (b).

Tabulation Of Data:



10/13

Name: _________________________________________ Class: ________

Conclusion:

_______________________________________________________________________________.

C) PRESSURE

1. Relationship Between Pressure And Force

Aim: ___________________________________________________________________________.

Problem statement: What is the relationship between pressure and force?

Hypothesis: _____________________________________________________________________.

Variable:

Manipulated : ____________________________________________________

Responding : ____________________________________________________

Constant : _____________________________________________________

Materials and apparatus:

1. ________________________

2. ________________________3. ________________________

4. ________________________

Procedure:

1. The apparatus are prepared as shown above.2. A 100g weight is placed on the sponge.

3. The distance of the compressed sponge is recorded.

4. Steps 2 and 3 are repeated with various loads of 200g, 300g, 400g and 500g.

5. The observation is recorded.

Tabulation Of Data:



11/13

Name: _________________________________________ Class: ________

Conlusion:

___________________________________________________________________

2. Relationship Between Pressure And surface Area.

Aim: __________________________________________________________________________

Problem statement: What is the relationship between pressure and surface area?

Hypothesis: ____________________________________________________________________.

Variable:

Manipulated : ____________________________________________________

Responding : ____________________________________________________

Constant : _____________________________________________________

Materials and apparatus: 1. ____________________ 2. _______________________

2. ____________________ 4. _______________________

Procedure:

1. A 500g weight on top of the plasticine as shown above. The impact on the plasticine is

recorded.

2. The experiment is repeated by putting the weight on a sharp nail and a blunt nail

respectively, while their pointed edges press against the plasticine.3. The dent formed on the plasticine are compared.

4. Result is recorded in a table.

Tabulation Of Data:



12/13

Name: _________________________________________ Class: ________

Conlusion:

__________________________________________________________________________.

Aim: To study Archimedes principle.

Problem statement: What is the relationship between the buoyant force and the weight of the water

displaced?Hypothesis: The buoyant force acting on the stone is equal to the weight of the water displaced by

the stone.

Variable:

Manipulated : Weight of stone

Responding : Weight of water displaced.

Constant : Type of water used

Materials and apparatus: 1. Eureka can, 2. Beaker3. Thread 4. Stones of different sizes

5. Spring balance 6. Water

Procedure:

1. Set up the apparatus as shown in the diagram above.

2. Record the weight of the stone as shown in diagram (a).

3. Measure the weight of the empty beaker and record it as W1.

4. Immerse the stone into the water in the Eureka Vessel as shown in diagram (b).

5. Record the weight of the stone when immersed in water.

6. Measure the weight of the beaker that contains the displaced water and record it as W2.

7. Calculate the weight of the displaced water. (W2 - W1 )



13/13

Name: _________________________________________ Class: ________

8. Repeat steps 2-7 by using different sizes of stones.

9. Record the observations in the form of the table.

Result:

Weight of stone in air = 0.8 N (W3)Weight of stone in water = 0.4 N (W4)

(appearance weight loss of the stone)Buoyant force acting on the stone = 0.4 N (W3 - W4)Weight of empty beaker = 0.6 N (W1)Weight of beaker and water displaced = 1.0 N (W 5)Weight of water displaced = 0.4 N (W5 - W1)From the results, W4 < W3

W3 - W4 = W5 - W1

Discussion:

Fromthe experiment, it is observed that volume of water displaced is equal to the volumeof the object that is completely immersed in water.

The weight of the stone when it is completely immersed in water is less than the natural

weight of the stone in air. This is due to the upthrust or buoyant force of the water acting on the stone.The amount of water in the beaker is the amount of water displaced by the stone. The

weight loss of the stone is equal to the weight of the water displaced.

Conlusion:

The buoyant force acting on the stone is equal to the weight of the water displaced by the

stone. Hypothesis is accepted.

Weight Of Stone in the water (N) Weight Of Water Displaced (N)

0.4 0.4

0.2 0.2

sc f5_practical student's module

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