SIDA/SAREC‐NICARAGUA30YEARS

Scien&ficachievementsfromtheInfec&ousDiseaseprogram

BACKGROUNDBACKGROUNDTheUniversitystrengtheningprograminUNAN‐LeónhassupportedthedevelopmentofTheUniversitystrengtheningprograminUNAN‐Leónhassupportedthedevelopmentofthreescien&ficprograms.threescien&ficprograms.

Thisposterpresentsthescien&ficresultsachievedbytheInfec&ousDiseaseprogramThisposterpresentsthescien&ficresultsachievedbytheInfec&ousDiseaseprogram

SUMMARYDuring the years of university strengthening the program has involved at least 100NicaraguanandSwedishscien&stsandresultedin(soon)8masterdisserta&ons9PhDdisserta&ons63scien&ficinterna&onalpublica&onsThemasterprograminMicrobiologyhasnowstartedinLeón

Licen&atesLicen&atesMastersMastersCorrales Corrales EdelmaEdelma , 1991 , 1991

Comparison on the performance ofComparison on the performance ofLeishmaniaLeishmania major Like and major Like and LeishmaniaLeishmaniabraziliensisbraziliensis As antigen for new world As antigen for new worldLeishmaniasisLeishmaniasis IgG- IgG-ImmunofluorescenceImmunofluorescence test. test.

Rivera Teresa, 1995Rivera Teresa, 1995 SeroepidemiologicalSeroepidemiological and Clinical study and Clinical study

of of ChagasChagas´ disease in Nicaragua.´ disease in Nicaragua.

MayorgaMayorga-Perez -Perez OrlandoOrlando, 1996, 1996Prevalence of antibodies to HepatitisPrevalence of antibodies to HepatitisA, B, C and E viruses in a healthyA, B, C and E viruses in a healthypopulation in León, Nicaragua.population in León, Nicaragua.

Paniagua Margarita, 1997Paniagua Margarita, 1997 Analysis of incidence of infection with Analysis of incidence of infection with

EnterotoxigenicEnterotoxigenic Escherichia coliEscherichia coli in a in aprospective cohort study of infantprospective cohort study of infantdiarrheadiarrhea in Nicaragua. in Nicaragua.

StructureofStructureofresearchresearch4 main areas:4 main areas:

Infantile diarrhoea Infantile diarrhoea - Bacteria - Bacteria - Virus - Virus Parasitic infections Parasitic infections Antimicrobial resistance Antimicrobial resistance Molecular biology Molecular biology

PhDPhDdisserta&onsatKarolinskaIns&tutetdisserta&onsatKarolinskaIns&tutetCáceresCáceres, Mercedes, 1999, Mercedes, 1999Ecological aspects of antimicrobial susceptibility of anaerobic bacteria in NicaraguaEcological aspects of antimicrobial susceptibility of anaerobic bacteria in Nicaragua

Espinoza, Felix, 2004Espinoza, Felix, 2004Rotavirus in pediatric gastroenteritis in Nicaraguan childrenRotavirus in pediatric gastroenteritis in Nicaraguan children

TéllezTéllez Sierra, Sierra, AleydaAleyda, 2006, 2006GiardiasisGiardiasis in Leon, Nicaragua : Prevalence and protection in Leon, Nicaragua : Prevalence and protection

BucardoBucardo-Rivera, -Rivera, FilemónFilemón, 2008, 2008Pediatric rotavirus and Pediatric rotavirus and norovirusnorovirus diarrhea in Nicaragua diarrhea in Nicaragua

LeivaLeiva, Byron, 2009, Byron, 2009AmoebiasisAmoebiasis : Diagnosis and prevalence in León Nicaragua : Diagnosis and prevalence in León Nicaragua

VilchezVilchez RugamaRugama, , BayardoBayardo Samuel, 2009 Samuel, 2009Molecular and phenotypic characterization of diarrheagenic Molecular and phenotypic characterization of diarrheagenic Escherichia coliEscherichia coli from Nicaraguan children from Nicaraguan children

Amaya-MayorgaAmaya-Mayorga, Erick, 2010-09-03, Erick, 2010-09-03Antibiotic resistance in gram negative bacteria affecting children from León, NicaraguaAntibiotic resistance in gram negative bacteria affecting children from León, Nicaragua

Reyes-Reyes-NavarreteNavarrete, Daniel, 2010-09-10, Daniel, 2010-09-10Escherichia coliEscherichia coli flora and diarrhea in Nicaraguan infants flora and diarrhea in Nicaraguan infants

Salazar-Anton, Fernando. 2011-06-17Salazar-Anton, Fernando. 2011-06-17Immunological and molecular characterization of Immunological and molecular characterization of onchosphereonchosphere antigens of antigens of TaeniaTaenia soliumsolium in Nicaragua in Nicaragua

Scien&ficpublica&onsinScien&ficpublica&onsinreviewedjournalsreviewedjournals

1.1. Linder E, Linder E, MarjaMarja IsaguilantsIsaguilants, Mats , Mats olssonolsson, Lena , Lena LundinLundin, , ShervanzindrouShervanzindrou, , EdelmaEdelma Corrales, Corrales, AleydaAleyda TéllezTéllez, Byron , Byron LeivaLeiva, William, WilliamMorales, Teresa Rivera and Jose M. Cabrera. Morales, Teresa Rivera and Jose M. Cabrera. AmebiasisAmebiasis in León, Nicaragua: in León, Nicaragua: EntamoebaeEntamoebae in stool examination and identification of in stool examination and identification ofAmebicAmebic Liver abscess Cases by Serology and PCR. Archives of Medical Research. Vo. 28, Suppl., pp. S314-S316. (1977) Liver abscess Cases by Serology and PCR. Archives of Medical Research. Vo. 28, Suppl., pp. S314-S316. (1977)

2.2. RalambosonRalamboson D, Le Bras J, D, Le Bras J, CoulangesCoulanges P, P, DeloronDeloron P, P, DulatDulat C, C, RamahatraRamahatra R, Morales W R, Morales W : Preliminary study of the prevalence of malaria: Preliminary study of the prevalence of malariainfestation in the Province of infestation in the Province of TamataveTamatave. Arch Inst Pasteur Madagascar. 50(1):9-14.(1982). Arch Inst Pasteur Madagascar. 50(1):9-14.(1982)

3.3. Celeste BJ, Celeste BJ, SoaresSoares GuimaraesGuimaraes MC S, Ma. Corrales E. MC S, Ma. Corrales E. PeroxidasePeroxidase antibody test for antibody test for mucocutaneousmucocutaneous LeishmaniasisLeishmaniasis serology serologyperformance indexes and comparison with a fluorescent antibody test. performance indexes and comparison with a fluorescent antibody test. Rev.InsRev.Ins. Med. Trop. Sao Paulo 30(6):411-414 . Med. Trop. Sao Paulo 30(6):411-414 novembro-novembro-dezembrodezembro. (1988). (1988)

4.4. Tellez A, L. Tellez A, L. CortézCortéz, A. , A. Aust-KettisAust-Kettis, G. , G. HuldtHuldt, J. Jonson and A. , J. Jonson and A. LofteniusLoftenius.: Antibodies against .: Antibodies against EntamoebaEntamoeba histolyticahistolytica antigens in sera antigens in serafrom individuals with from individuals with amoebiasisamoebiasis of different localization. Archives of Medical Research Vo. 21: ( of different localization. Archives of Medical Research Vo. 21: ( SupplSuppl 1): 63. (1990) 1): 63. (1990)

5.5. Caceres. M., Caceres. M., PalmgrenPalmgren, A-C and Nord C. E. Antimicrobial resistance in human anaerobic , A-C and Nord C. E. Antimicrobial resistance in human anaerobic microfloramicroflora in Nicaragua and Sweden. in Nicaragua and Sweden.Spanish Journal of Chemotherapy 4:151-155. (1991) Spanish Journal of Chemotherapy 4:151-155. (1991) CáceresCáceres. M., . M., HedbergHedberg, M., , M., LindqvistLindqvist, L., Nord, , L., Nord, C.E.:PurificationC.E.:Purification and andCharacterization of a beta-Characterization of a beta-lactamaselactamase from from BacteroidesBacteroides distasonisdistasonis. Journal of Chemotherapy 3(suppl): 121-124. (1991). Journal of Chemotherapy 3(suppl): 121-124. (1991)

6.6. Soares Guimaraes MC., Celeste BJ, Ma. Soares Guimaraes MC., Celeste BJ, Ma. Corrales E , Corrales E , AntunesAntunes C.ComparisonC.Comparison on the performance of on the performance of LeishmaniaLeishmania major Like and major Like andLeishmaniaLeishmania braziliensisbraziliensis As antigen for new world As antigen for new world LeishmaniasisLeishmaniasis IgG- IgG-ImmunofluorescenceImmunofluorescence test. Rev. Inst. Med. Trop. Sao Paulo test. Rev. Inst. Med. Trop. Sao Paulo33(6):503-508. (1991)33(6):503-508. (1991)

7.7. TéllezTéllez A., Cortez. L., A., Cortez. L., KettisKettis A and A and HuldtHuldt G.AmebiasisG.Amebiasis in Nicaragua: Class Specific Serum in Nicaragua: Class Specific Serum Antibody.:ArchivesAntibody.:Archives of Medical Research Vo. of Medical Research Vo.23, No. 2, pp 261-264, (1992)23, No. 2, pp 261-264, (1992)

8.8. BeneditoBenedito A. Peres, UE. A. Peres, UE. ConfloneieConfloneie. Pedro. P. . Pedro. P. ChieffiChieffi. L.M. . L.M. GerkienGerkien, , EdelmaEdelma Corrales, Carmen S. Guerra y Marcelo M. Corrales, Carmen S. Guerra y Marcelo M. BrandaoBrandao.. Estudo EstudoDa Frecuencia de Anticorpos Anti-Toxocara canis em populacho indígena. Da Frecuencia de Anticorpos Anti-Toxocara canis em populacho indígena. Rev. Inst. Med. Trop. Sao Paulo 35(Suppl. 10). (1993)Rev. Inst. Med. Trop. Sao Paulo 35(Suppl. 10). (1993)

9.9. SoaresSoares GuimaraesGuimaraes MC , Celeste BJ , Ma. MC , Celeste BJ , Ma. Corrales E : Antigenos de L. major-like e L.b. braziliensis Na Reacao de ImnufluorescenciaCorrales E : Antigenos de L. major-like e L.b. braziliensis Na Reacao de Imnufluorescencia(IgG-IF) Na Leismaniose Mucocutanea. (IgG-IF) Na Leismaniose Mucocutanea. Rev. Inst. Med. Rev. Inst. Med. TropTrop Sao Paulo 35(Suppl.10) (1993) Sao Paulo 35(Suppl.10) (1993)

10.10.Medina EC, Medina EC, WidmalmWidmalm G, G, WeintraubWeintraub A, Vial PA, Levine MM, Lindberg A: Structural studies of the O-antigenic polysaccharides of A, Vial PA, Levine MM, Lindberg A: Structural studies of the O-antigenic polysaccharides ofEscherichia coli 03 and the Escherichia coli 03 and the enteroaggregativeenteroaggregative Escherichia coli strain 17-2.Eur J Escherichia coli strain 17-2.Eur J BiochemBiochem 224:191-196.(1994) 224:191-196.(1994)

11.11.RatnayakeRatnayake S, S, WeintraubWeintraub A, Medina EC. Structural studies of the c 090 O-antigen A, Medina EC. Structural studies of the c 090 O-antigen polysaccharide.Carbohydrpolysaccharide.Carbohydr Res, 263:209-215.(1994) Res, 263:209-215.(1994)12.12.Rivera T. Palma G. R. y Morales W. Rivera T. Palma G. R. y Morales W. SeroepidemiologicalSeroepidemiological and Clinical study of and Clinical study of ChagasChagas´ disease in Nicaragua. Rec. Inst. Med. Trop.´ disease in Nicaragua. Rec. Inst. Med. Trop.

Sao Paulo 37(3): 207-213. (1995)Sao Paulo 37(3): 207-213. (1995)13.13.Palma-Guzman Palma-Guzman RosárioRosário, Teresa Rivera B and William Morales G. Domestic vectors of , Teresa Rivera B and William Morales G. Domestic vectors of ChagasChagas’’ disease in three rural communities of disease in three rural communities of

Nicaragua. Rev. Inst. Med. Trop. Sao Paulo 38 (2): 133-140,(1996)Nicaragua. Rev. Inst. Med. Trop. Sao Paulo 38 (2): 133-140,(1996)14.14.MayorgaMayorga-Perez -Perez OrlandoOrlando, William Morales, Margarita , William Morales, Margarita PaniaguaPaniagua and and OrjanOrjan StrannegardStrannegard. Prevalence of antibodies to Hepatitis A, B, C. Prevalence of antibodies to Hepatitis A, B, C

and E viruses in a healthy population in León, Nicaragua. Am. J .Trop. Med. and E viruses in a healthy population in León, Nicaragua. Am. J .Trop. Med. HygHyg. 55(1): 17-21, (1996). 55(1): 17-21, (1996)15.15.Herrmann Herrmann BjörnBjörn, Felix Espinoza, , Felix Espinoza, RosauraRosaura Rivera Villegas, George Davey Smith, Angela Ramos, Matthias Egger: Rivera Villegas, George Davey Smith, Angela Ramos, Matthias Egger:

Genital Genital chlamydiachlamydia infection among women in Nicaragua: validity of direct fluorescent antibody testing, prevalence, risk factors and infection among women in Nicaragua: validity of direct fluorescent antibody testing, prevalence, risk factors andclinical manifestations. Genitourinary Med. 72: 20-26,(1996)clinical manifestations. Genitourinary Med. 72: 20-26,(1996)

16.16.KjellbergKjellberg A, A, UrbinaUrbina F, F, WeintraubWeintraub A, A, WidmalmWidmalm G.: Structural studies of the O-antigenic polysaccharide from the G.: Structural studies of the O-antigenic polysaccharide from the enteropathogenicenteropathogenicEscherichia coli O125.Eur J Escherichia coli O125.Eur J BiochemBiochem 239:532-538 (1996) 239:532-538 (1996)

17.17.StaafStaaf M, M, UrbinaUrbina F, F, WidmalmWidmalm G. Structural studies of G. Structural studies of ofof the the enteroinvasiveenteroinvasive EcherichiaEcherichia coli (EIEC ) from the coli (EIEC ) from the enterotoxigenicenterotoxigenicEscherichia coli ( ETEC ) O101. Escherichia coli ( ETEC ) O101. CarbohydrCarbohydr Res, 291/127-139.(1996) Res, 291/127-139.(1996)

18.18.Espinoza Felix, Margarita Espinoza Felix, Margarita PaniaguaPaniagua, Hans , Hans HallanderHallander, , LennartLennart SvenssonSvensson, , OrjanOrjan StrannegardStrannegard. Rotavirus Infections in young. Rotavirus Infections in youngNicaraguan Children. Nicaraguan Children. PediatrPediatr Infect Disease Journal 16: 564-71,(1997) Infect Disease Journal 16: 564-71,(1997)

19.19.Espinoza Felix, Margarita Espinoza Felix, Margarita PaniaguaPaniagua, Hans , Hans HallanderHallander, K.O. , K.O. HedlundHedlund and and LennartLennart SvenssonSvensson. Prevalence and characteristics of severe. Prevalence and characteristics of severerotavirus infections in Nicaraguan children. Annals of tropical paediatrics 17:25-32, (1997)rotavirus infections in Nicaraguan children. Annals of tropical paediatrics 17:25-32, (1997)

20.20.PaniaguaPaniagua M., F. Espinoza, M. M., F. Espinoza, M. RingmanRingman, E. , E. ReizensteinReizenstein, A. M. , A. M. SvennerholmSvennerholm, and H. , and H. HallanderHallander. Analysis of incidence of infection with. Analysis of incidence of infection withEnterotoxigenicEnterotoxigenic Escherichia coli in a prospective cohort study of infant Escherichia coli in a prospective cohort study of infant diarrheadiarrhea in Nicaragua. Journal of Clinical Microbiology in Nicaragua. Journal of Clinical Microbiology35:1404-1410,(l997)35:1404-1410,(l997)

21.21.Linder Linder EwertEwert, , MarjaMarja IsaguliantsIsaguliants, Mats Olsson, Lena , Mats Olsson, Lena LundinLundin, , ShervanShervan ZindrouZindrou, , EdelmaEdelma Corrales, Corrales, AleidaAleida Tellez, Byron Tellez, Byron leivaleiva, William, WilliamMorales, Teresa Rivera, and Jose M Cabrera. Morales, Teresa Rivera, and Jose M Cabrera. AmebiasisAmebiasis in León, Nicaragua: in León, Nicaragua: EntamoebaeEntamoebae in Stool Examination and Identification of in Stool Examination and Identification ofAmoebic Liver Abscess Cases by Serology and Amoebic Liver Abscess Cases by Serology and PCR.ArchivesPCR.Archives of Medical Research 28, Suppl. pp. S314-316, (1997) of Medical Research 28, Suppl. pp. S314-316, (1997)

22.22.Tellez A., Tellez A., W.MoralesW.Morales, , T.RiveraT.Rivera, , E.MeyerE.Meyer, , B.LeivaB.Leiva, , E.LinderE.Linder: Prevalence of intestinal parasites in the human population of León,: Prevalence of intestinal parasites in the human population of León,Nicaragua. Nicaragua. ActaActa TropicaTropica 66: 119-125, (1997) 66: 119-125, (1997)

23.23.Felix Espinoza, Margarita Felix Espinoza, Margarita PaniaguaPaniagua, Hans , Hans HallanderHallander, , LennartLennart SvenssonSvensson, , OrjanOrjan StrannegardStrannegard: Rotavirus Infections in young: Rotavirus Infections in youngNicaraguan Nicaraguan Children.PediatrChildren.Pediatr Infect Disease Journal, 16: 564-71. ( 1997) Infect Disease Journal, 16: 564-71. ( 1997)

24.24.CáceresCáceres. M., . M., CareraCarera, E., , E., PalmgrenPalmgren, A-C and Nord C. E. Antimicrobial susceptibility of anaerobic bacteria from the intestinal , A-C and Nord C. E. Antimicrobial susceptibility of anaerobic bacteria from the intestinal microfloramicrofloraof healthy children and antimicrobial-treated children in Nicaragua. Spanish Journal of Chemotherapy 3:221-228. (1998)of healthy children and antimicrobial-treated children in Nicaragua. Spanish Journal of Chemotherapy 3:221-228. (1998)

25.25.Johansen K, Johansen K, HinkulaHinkula J, Espinoza F, Levi M, J, Espinoza F, Levi M, ZengZeng C, C, RundenRunden U, U, VesikariVesikari T, Estes M and T, Estes M and SvenssonSvensson L. Humoral and cell-mediated L. Humoral and cell-mediatedimmune responses to the NSP4 enterotoxin of rotavirus. Journal of Medical Virology 59:369-377, (1999.)immune responses to the NSP4 enterotoxin of rotavirus. Journal of Medical Virology 59:369-377, (1999.)

26.26.CáceresCáceres. M., . M., CareraCarera, E., Palma, A., , E., Palma, A., BerriosBerrios, G., , G., WintraubWintraub, A., and Nord C. E. Antimicrobial susceptibility of anaerobic and aerobic, A., and Nord C. E. Antimicrobial susceptibility of anaerobic and aerobicbacteria isolated from mixed infections in Nicaragua. Spanish Journal of Chemotherapy 12:332-339 .(1999)bacteria isolated from mixed infections in Nicaragua. Spanish Journal of Chemotherapy 12:332-339 .(1999)

27.27.StaafStaaf M, M, UrbinaUrbina F, F, WeintraubWeintraub A, A, WidmalmWidmalm G.: Structural elucidation of the O-antigenic polysaccharide from Escherichia coli O21 and G.: Structural elucidation of the O-antigenic polysaccharide from Escherichia coli O21 andthe the enteroaggregativeenteroaggregative Escherichia coli strain 105. Escherichia coli strain 105. EurEur J J BiochemBiochem 266:241-245 (1999) 266:241-245 (1999)

28.28.StaafStaaf M, M, UrbinaUrbina F, F, WeintraubWeintraub A, A, WidmalmWidmalm G. Structure elucidation of the O-antigenic polysaccharide from the G. Structure elucidation of the O-antigenic polysaccharide from the enteroaggregativeenteroaggregativeEscherichia coli strain 62D1. Escherichia coli strain 62D1. EurEur J J BiochemBiochem , 262:56-62 (1999) , 262:56-62 (1999)

29.29.S. S. ZindrauZindrau, P. , P. HagblomHagblom, B. , B. LeivaLeiva, , TéllezTéllez A, JM Cabrera, A. A, JM Cabrera, A. MatuteMatute and E. Linder. Antibodies against and E. Linder. Antibodies against EntamoebaEntamoeba histolyticahistolytica hemolisinhemolisinHLY4 in sera from patients with invasive HLY4 in sera from patients with invasive amebiasisamebiasis. Archives of Medical Research. (2000). Archives of Medical Research. (2000)

30.30.CáceresCáceres. M., Zhang, G., . M., Zhang, G., WeintraubWeintraub, A., Nord, C.E.: Prevalence and antimicrobial susceptibility patter of , A., Nord, C.E.: Prevalence and antimicrobial susceptibility patter of EnterotoxigenicEnterotoxigenic BacteroidesBacteroidesfragilisfragilis in children with in children with diarrheadiarrhea in Nicaragua. Anaerobe, in Nicaragua. Anaerobe, junejune .6:122-126. (2000) .6:122-126. (2000)

31.31.Zindrou S, Orozco E, Linder E, Téllez A, Björkman A. Zindrou S, Orozco E, Linder E, Téllez A, Björkman A. Specific detection of Entamoeba histolytica DNA by hemolysin gene targetedPCR. ActaActa Trop. Feb 23;78(2):117-25.(2001) Trop. Feb 23;78(2):117-25.(2001)

32.32.HallanderHallander HO, HO, PaniaguaPaniagua M, Espinoza F, M, Espinoza F, AskelöfAskelöf P, Corrales E, P, Corrales E, RingmanRingman M, M, StorsaeterStorsaeter J. Calibrated serological techniques J. Calibrated serological techniquesdemonstrate significant different serum response rates to an oral killed cholera vaccine between Swedish and Nicaraguan children.demonstrate significant different serum response rates to an oral killed cholera vaccine between Swedish and Nicaraguan children.Vaccine 21:138-145,(2002)Vaccine 21:138-145,(2002)

33.33.TéllezTéllez A, A, Winiecka-KrusnellWiniecka-Krusnell J, J, PaniaguaPaniagua M , Linder E. Antibodies in M , Linder E. Antibodies in mother´smother´s milk protect children against milk protect children against giardiasisgiardiasis..Scand J Infect Scand J Infect DisDis 35:322-325,(2003) 35:322-325,(2003)

34.34.MalmbergMalmberg LovisaLovisa, Caroline , Caroline FockFock, Felix Espinoza, , Felix Espinoza, FilemonFilemon BucardoBucardo, Ann Christine , Ann Christine SyvänenSyvänen and and KåreKåre BondesonBondeson. Microarrays of. Microarrays ofgenotyping of human group A rotavirus by multiplex capture and type specific primer extension. Journal of Clinical Microbiologygenotyping of human group A rotavirus by multiplex capture and type specific primer extension. Journal of Clinical Microbiology41(11): 5153-5158, (2003)41(11): 5153-5158, (2003)

35.35.LovmarLovmar L, L, FockFock C, Espinoza F, C, Espinoza F, BucardoBucardo F, F, SyvänenSyvänen AC, AC, BondesonBondeson K. K. Microarrays for genotyping human group a rotavirus byMicroarrays for genotyping human group a rotavirus bymultiplex capture and type-specific primer extension.multiplex capture and type-specific primer extension. J J ClinClin MicrobiolMicrobiol. Nov;41(11):5153-8 (2003). Nov;41(11):5153-8 (2003)

36.36.MayorgaMayorga O, M. Egger, M. O, M. Egger, M. ZellmeyerZellmeyer and cols. Efficacy of a and cols. Efficacy of a virosomevirosome hepatitis A vaccine in an endemic region in Nicaragua: A hepatitis A vaccine in an endemic region in Nicaragua: Arandomized placebo-controlled trial. Journal Infectious diseases (2003)randomized placebo-controlled trial. Journal Infectious diseases (2003)

37.37.Larsson EA, Larsson EA, UrbinaUrbina F, Yang Z, F, Yang Z, WeintraubWeintraub A, A, WidmalmWidmalm G. Structural and immunochemical relationship between the O-antigenic G. Structural and immunochemical relationship between the O-antigenicpolysaccharides from the polysaccharides from the enteroaggregativeenteroaggregative Escherichia coli strain 396/C1 and Escherichia coli O126. Carbohydrate Research, .Jun Escherichia coli strain 396/C1 and Escherichia coli O126. Carbohydrate Research, .Jun1;339(8):1491-6.(2004)1;339(8):1491-6.(2004)

38.38.Caceres M. Bacteraemia neonatal Caceres M. Bacteraemia neonatal porpor EnterobacterEnterobacter multirresistentesmultirresistentes productoresproductores de Beta- de Beta-lactamasalactamasa de de expectroexpectro extendidoextendido..RevistaRevista MédicaMédica LatinoamericanaLatinoamericana (2004 (2004

39.39.Ali T, Ali T, UrbinaUrbina F F, , WeintraubWeintraub A A, , WidmalmWidmalm G G. Structural studies of the O-antigenic polysaccharides from the . Structural studies of the O-antigenic polysaccharides from the enteroaggregativeenteroaggregative E coli E colistrain 522/C1 and the international type strain from Escherichia coli O 178. strain 522/C1 and the international type strain from Escherichia coli O 178. CarbohydrCarbohydr Res. Res. 340(12): 2010-4,(2005) 340(12): 2010-4,(2005)

40.40.BucardoBucardo F F, , Meza-Lucas AMeza-Lucas A, Espinoza F, , Espinoza F, Garcia-Garcia-JeronimoJeronimo RC RC, , Garcia-Garcia-RodeaRodea R R, , Correa DCorrea D. The . The seroprevalenceseroprevalence of of TaeniaTaenia soliumsoliumcysticercosiscysticercosis among epileptic patients in Leon, Nicaragua, as evaluated by ELISA and western blotting. among epileptic patients in Leon, Nicaragua, as evaluated by ELISA and western blotting. Ann Ann TropTrop Med Med ParasitolParasitol..99(1): 41-5, (2005)99(1): 41-5, (2005)

41.41.Tellez A, Tellez A, Palm DPalm D, Weiland M, , Weiland M, Aleman JAleman J, , Winiecka-Krusnell JWiniecka-Krusnell J, , Linder ELinder E, , Svard SSvard S. S. Secretoryecretory antibodies against antibodies against GiardiaGiardia intestinalisintestinalis in inlactating Nicaraguan women. lactating Nicaraguan women. Parasite Parasite ImmunolImmunol.. 27(5): 163-9, (2005) 27(5): 163-9, (2005)

42.42.UrbinaUrbina F F, , NordmarkNordmark EL EL, , Yang ZYang Z, , WeintraubWeintraub A A, , ScheutzScheutz F F, , WidmalmWidmalm G G. Structural elucidation of the O-antigenic polysaccharide from. Structural elucidation of the O-antigenic polysaccharide fromthe the enteroaggregativeenteroaggregative E coli strain 180/C3 and its immunochemical relationship with E. coli O5 and O65. E coli strain 180/C3 and its immunochemical relationship with E. coli O5 and O65. CarbohydrCarbohydr Res. Res. 340(4): 645- 340(4): 645-50, (2005)50, (2005)

43.43.LeivaLeiva Byron, Marianne Byron, Marianne LebbadLebbad, Jadwiga , Jadwiga Winiecka-KrusnellWiniecka-Krusnell, Isabel , Isabel AltamiranoAltamirano., ., AleydaAleyda TèllezTèllez and and EwertEwert Linder. Linder. OverdiagnosisOverdiagnosis of ofEntamoebaEntamoeba histolyticahistolytica and and EntamoebaEntamoeba dispardispar in Nicaragua: A microscopic, Triage Parasite Panel and PCR study. in Nicaragua: A microscopic, Triage Parasite Panel and PCR study. Arch Med Res.Arch Med Res.May;37(4):529-34.(2006)May;37(4):529-34.(2006)

44.44.Rodríguez-DíazRodríguez-Díaz J, J, BanasazBanasaz M, M, IstrateIstrate C, C, BuesaBuesa J, Lundgren O, Espinoza F, J, Lundgren O, Espinoza F, SundqvistSundqvist T, T, RottenbergRottenberg M, M, SvenssonSvensson L. L. Role of nitricRole of nitricoxide during rotavirus infection.oxide during rotavirus infection. J Med J Med VirolVirol. Jul;78(7):979-85.(2006). Jul;78(7):979-85.(2006)

45.45.Espinoza F, Espinoza F, BucardoBucardo F, F, PaniaguaPaniagua M, M, SvenssonSvensson L, L, HallanderHallander HO, HO, BondesonBondeson K. K. Shifts of rotavirus g and p types in Nicaragua--2001-Shifts of rotavirus g and p types in Nicaragua--2001-2003.2003. PediatrPediatr Infect Infect DisDis J. Nov;25(11):1078-80. (2006) J. Nov;25(11):1078-80. (2006)

46.46.Ruiz-Palacios GM, Ruiz-Palacios GM, Pérez-SchaelPérez-Schael I, Velázquez FR, Abate H, Breuer T, Clemens SC, I, Velázquez FR, Abate H, Breuer T, Clemens SC, CheuvartCheuvart B, Espinoza F, Gillard P, B, Espinoza F, Gillard P, InnisInnis BL, BL,Cervantes Y, Cervantes Y, LinharesLinhares AC, AC, LópezLópez P, P, MacíasMacías-Parra M, Ortega--Parra M, Ortega-BarríaBarría E, Richardson V, Rivera-Medina DM, Rivera L, Salinas B, E, Richardson V, Rivera-Medina DM, Rivera L, Salinas B, Pavía-Pavía-RuzRuz N, N, SalmerónSalmerón J, J, RüttimannRüttimann R, R, TinocoTinoco JC, Rubio P, JC, Rubio P, NuñezNuñez E, Guerrero ML, E, Guerrero ML, YarzábalYarzábal JP, JP, DamasoDamaso S, S, TornieporthTornieporth N, N, Sáez-LlorensSáez-Llorens X, X,VergaraVergara RF, RF, VesikariVesikari T, T, BouckenoogheBouckenooghe A, Clemens R, De A, Clemens R, De VosVos B, B, O'RyanO'Ryan M; Human Rotavirus Vaccine Study Group. M; Human Rotavirus Vaccine Study Group. Safety andSafety andefficacy of an attenuated vaccine against severe rotavirus gastroenteritis.efficacy of an attenuated vaccine against severe rotavirus gastroenteritis. N N EnglEngl J Med. Jan 5;354(1):11-22.( 2006) J Med. Jan 5;354(1):11-22.( 2006)

47.47.BucardoBucardo F, F, KarlssonKarlsson B, B, NordgrenNordgren J, J, PaniaguaPaniagua M, M, GonzálezGonzález A, Amador JJ, Espinoza F, A, Amador JJ, Espinoza F, SvenssonSvensson L. L. Mutated G4P[8] rotavirusMutated G4P[8] rotavirusassociated with a nationwide outbreak of gastroenteritis in Nicaragua in 2005.associated with a nationwide outbreak of gastroenteritis in Nicaragua in 2005. J J ClinClin MicrobiolMicrobiol. Mar;45(3):990-7.(2007). Mar;45(3):990-7.(2007)

48.48.BucardoBucardo F, F, KarlssonKarlsson B, B, NordgrenNordgren J, J, PaniaguaPaniagua M, M, GonzálezGonzález A, Amador JJ, Espinoza F, A, Amador JJ, Espinoza F, SvenssonSvensson L. L. Mutated G4P[8] rotavirusMutated G4P[8] rotavirusassociated with a nationwide outbreak of gastroenteritis in Nicaragua in 2005.associated with a nationwide outbreak of gastroenteritis in Nicaragua in 2005. J J ClinClin MicrobiolMicrobiol. Mar;45(3):990-7.(2007). Mar;45(3):990-7.(2007)

49.49.LinharesLinhares AC, Velázquez FR, AC, Velázquez FR, Pérez-SchaelPérez-Schael I, I, Sáez-LlorensSáez-Llorens X, Abate H, Espinoza F, X, Abate H, Espinoza F, LópezLópez P, P, MacíasMacías-Parra M, Ortega--Parra M, Ortega-BarríaBarría E, Rivera- E, Rivera-Medina DM, Rivera L, Medina DM, Rivera L, Pavía-RuzPavía-Ruz N, N, NuñezNuñez E, E, DamasoDamaso S, Ruiz-Palacios GM, De S, Ruiz-Palacios GM, De VosVos B, B, O'RyanO'Ryan M, Gillard P, M, Gillard P, BouckenoogheBouckenooghe A; Human A; HumanRotavirus Vaccine Study Group. Rotavirus Vaccine Study Group. Efficacy and safety of an oral live attenuated human rotavirus vaccine against rotavirusEfficacy and safety of an oral live attenuated human rotavirus vaccine against rotavirusgastroenteritis during the first 2 years of life in Latin American infants: a randomised, double-blind, placebo-controlled phase IIIgastroenteritis during the first 2 years of life in Latin American infants: a randomised, double-blind, placebo-controlled phase IIIstudy.study. Lancet. Apr 5;371(9619):1181-9.(2008) Lancet. Apr 5;371(9619):1181-9.(2008)

50.50.BucardoBucardo F, F, NordgrenNordgren J, J, CarlssonCarlsson B, B, PaniaguaPaniagua M, Lindgren PE, Espinoza F, M, Lindgren PE, Espinoza F, SvenssonSvensson L. L. PediatricPediatric norovirusnorovirus diarrheadiarrhea in Nicaragua. in Nicaragua. J JClinClin MicrobiolMicrobiol. Aug;46(8):2573-80. (2008). Aug;46(8):2573-80. (2008)

51.51.NordgrenNordgren J, J, BucardoBucardo F, F, DienusDienus O, O, SvenssonSvensson L, Lindgren PE. L, Lindgren PE. Novel light-upon-extension real-time PCR assays for detection andNovel light-upon-extension real-time PCR assays for detection andquantification of quantification of genogroupgenogroup I and II I and II norovirusesnoroviruses in clinical specimens. in clinical specimens. J J ClinClin MicrobiolMicrobiol. Jan;46(1):164-70 (2008). Jan;46(1):164-70 (2008)

52.52.LebbadLebbad M, M, AnkarklevAnkarklev J, Tellez A, J, Tellez A, LeivaLeiva B, B, AnderssonAndersson JO, JO, SvärdSvärd S. S. Dominance of Dominance of GiardiaGiardia assemblage B in León, Nicaragua. assemblage B in León, Nicaragua. ActaActaTrop.Apr;106(1):44-53.(2008)Trop.Apr;106(1):44-53.(2008)

53.53.LeivaLeiva B, B, ClasdotterClasdotter E, Linder E, E, Linder E, Winiecka-KrusnellWiniecka-Krusnell J. J. Free-living Free-living AcanthamoebaAcanthamoeba and and NaegleriaNaegleria spp. spp. amebaeamebae in water sources of León, in water sources of León,Nicaragua.Nicaragua. Rev Rev BiolBiol Trop. Jun;56(2):439-46.( 2008) Trop. Jun;56(2):439-46.( 2008)

54.54.Linder E, Linder E, LundinLundin M, M, ThorsThors C, C, LebbadLebbad M, M, Winiecka-KrusnellWiniecka-Krusnell J, J, HelinHelin H, H, LeivaLeiva B, B, IsolaIsola J, J, LundinLundin J. J. Web-based virtual microscopy forWeb-based virtual microscopy forparasitologyparasitology: a novel tool for education and quality assurance.: a novel tool for education and quality assurance. PLoSPLoS NeglNegl TropTrop Dis. ;2(10):e315.(2008) Dis. ;2(10):e315.(2008)

55.55.LebbadLebbad M, M, AnkarklevAnkarklev J, Tellez A, J, Tellez A, LeivaLeiva B, B, AnderssonAndersson JO, JO, SvärdSvärd S. S. Dominance of Dominance of GiardiaGiardia assemblage B in León, assemblage B in León, Nicaragua.Nicaragua.ActaActaTrop. Apr;106(1):44-53. (2008)Trop. Apr;106(1):44-53. (2008)

56.56.AmayaAmaya E, Caceres M, Fang H, Ramirez AT, E, Caceres M, Fang H, Ramirez AT, PalmgrenPalmgren AC, Nord CE, AC, Nord CE, WeintraubWeintraub A. A. Extended-spectrum beta-Extended-spectrum beta-lactamaselactamase-producing-producingKlebsiellaKlebsiella pneumoniaepneumoniae in a neonatal intensive care unit in León, Nicaragua. in a neonatal intensive care unit in León, Nicaragua. IntInt J J AntimicrobAntimicrob Agents. Apr;33(4):386-7 (2009) Agents. Apr;33(4):386-7 (2009)

57.57.BucardoBucardo F, F, KindbergKindberg E, E, PaniaguaPaniagua M, M, GrahnGrahn A, Larson G, A, Larson G, VildevallVildevall M, M, SvenssonSvensson L. L. Genetic susceptibility to symptomatic Genetic susceptibility to symptomatic norovirusnorovirusinfection in Nicaragua.infection in Nicaragua. J Med J Med VirolVirol. Apr;81(4):728-35. (2009). Apr;81(4):728-35. (2009)

58.58.Vilchez S, Reyes D, Paniagua M, Bucardo F, Möllby R, Weintraub A. Vilchez S, Reyes D, Paniagua M, Bucardo F, Möllby R, Weintraub A. Prevalence of Prevalence of diarrhoeagenicdiarrhoeagenic Escherichia coli in children from Escherichia coli in children fromLeón, Nicaragua.León, Nicaragua. J Med J Med MicrobiolMicrobiol. May;58(Pt 5):630-7.( 2009). May;58(Pt 5):630-7.( 2009)

59.59.Reyes D, Vilchez S, Paniagua M, Colque P, Weintraub A, Möllby R, Kühn I. Reyes D, Vilchez S, Paniagua M, Colque P, Weintraub A, Möllby R, Kühn I. Diversity of intestinal Escherichia coli populations inDiversity of intestinal Escherichia coli populations inNicaraguan children with and without diarrhoea.Nicaraguan children with and without diarrhoea. J Med J Med MicrobiolMicrobiol. Dec;58(Pt 12):1593-600. (2009). Dec;58(Pt 12):1593-600. (2009)

60.60.VilchezVilchez S, S, LundborgLundborg M, M, UrbinaUrbina F, F, WeintraubWeintraub A, A, WidmalmWidmalm G. Structural studies of the O-antigenic polysaccharides from the G. Structural studies of the O-antigenic polysaccharides from theenteroaggregativeenteroaggregative Escherichia coli strain 94/D4 and the international type strain Escherichia coli O82. Escherichia coli strain 94/D4 and the international type strain Escherichia coli O82. CarbohydrCarbohydr Res. Dec Res. Dec14;344(18):2528-32. (2009)14;344(18):2528-32. (2009)

61.61.AmayaAmaya E, Caceres M, E, Caceres M, FancFanc H, Torres Ramirez A, H, Torres Ramirez A, PalmgrenPalmgren AC, Nord CE, AC, Nord CE, WeintraubWeintraub A. Antibiotic resistance patterns in gram- A. Antibiotic resistance patterns in gram-negative and gram-positive bacteria causing negative and gram-positive bacteria causing septicemiasepticemia in newborns in León, Nicaragua: correlation with environmental samples J in newborns in León, Nicaragua: correlation with environmental samples JChemotherChemother. 2010 Feb;22(1):25-9.(2010). 2010 Feb;22(1):25-9.(2010)

62.62.Nordgren J, Bucardo F, Svensson L, Lindgren PE. Nordgren J, Bucardo F, Svensson L, Lindgren PE. Novel Light-Upon-Extension Real-Time PCR Assay for Simultaneous Detection,Novel Light-Upon-Extension Real-Time PCR Assay for Simultaneous Detection,Quantification and Quantification and GenogroupingGenogrouping of Group A Rotavirus. of Group A Rotavirus. J J ClinClin Microbiol.MarMicrobiol.Mar 10. (2010) 10. (2010)

63.63.SvenssonSvensson L, L, KindbergKindberg E, E, NordgrenNordgren S, S, CarlssonCarlsson B, B, MollbyMollby R, R, PaniaguaPaniagua M. Asymptomatic Norovirus infections in Nicaraguan children M. Asymptomatic Norovirus infections in Nicaraguan childrenand its association with viral properties and and its association with viral properties and histohisto-blood group antigens. -blood group antigens. Pediatric Infectious Disease Journal accepted 2010Pediatric Infectious Disease Journal accepted 2010

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Number of publications

1977-1989

1990-1994

1995-1999

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2006-2010

Scientific publications