Simultaneous Measurement of Necrotic,Apoptotic Dead and Live Cells

Necrotic/Apoptotic DeadTreat Cells with DNAse to nick cells

Incorporate FITC-UTP with TdT

Apoptotic LiveFix cells and permeabilize

Incorporate B-UTP with TdT thenPE-Avidin

10 10 10 10 100 1 2 3 4

TDT48015FITC UTP ->

0 256 512 768 1024

TDT48015FSC ->

SIMULTANEOUS EVALUATION OF DEAD, NECROTIC AND APOPTOTIC CELLS

R4

R1 R2

R3

PE-d

UTP

Fl-12-dUTP

A

FSC

SS

C

gated R1

B

FSC

SS

C

gated R3

C

FSC

SS

C

gated R2

D

LIGHT SCATTER ANALYSIS OF DEAD CELLS AND VIABLE APOPTOTIC CELLS

10 1 10 2 10 3 10 4

FL3-Height -->

10

110

210

310

4FL2

-Heig

ht

-->

FOUR COLOR DETECTION OF SURFACE ANTIGENS, APOPTOSIS AND DEAD CELLS

10 20 30 40 50 60 70 80 90 100 120

FL4-HEIGHT -->

10

110

210

310

4FL

1-H

eig

ht

-->

10 20 30 40 50 60 70 80 90 100 120

FL4-HEIGHT -->

10

110

210

310

4FL

1-H

eig

ht

-->

PE-C

D19

DEA

D C

ELLS

DEA

D C

ELLS

TC-CD33 APOPTOSIS APOPTOSIS