Single-point Calibration and its Error

Creating a calibration curve

based on one standard Use the scroll bar to adjust the slope or error for the single-point calibration

and the origin (0,0). slope = 17.0 of the single-point calabration curve

single-point

2380 absorbance line c = 0.035

calibration curve 0 0.37 A = 0.60

c A 0 0 0.05 0.37

0 0 0.035 0.595 single-point cal curve

0.010 0.13 0.02176 0.37 0.025 0.37

0.020 0.28 slope = 17 0.02176 0 0.025 0

0.030 0.45

0.040 0.60 c = 0.02176

0.050 0.74

your unknown A = 0.37 0.022 %error = -12.9

absorbance 0.025

Idea from Barb Gage

For a single point of known concentration and measured absorbance, the red diamond

and the origin (0,0) form a line as shown by the brown line. How does error influence the line?

csingle point =

ccal curve =

Sinex 2007

0.000 0.010 0.020 0.030 0.040 0.0500.00

0.20

0.40

0.60

0.80

f(x) = 14.8 xR² = 0.99943587190974

f(x) = 17 xR² = 1

concentration, M

A