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SlideViewer 2.5 User's Guide June 2, 2021 Rev. 1

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Page 1: SlideViewer 2.5 User's Guide

SlideViewer 2.5User's Guide

June 2, 2021Rev. 1

Page 2: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide

Table of Contents1 Installaton of SlideViewer...............................................................................................6

1.1 System requirements.........................................................................................................6

1.2 Installatin rioedure........................................................................................................6

2 Using SlideViewer..........................................................................................................16

2.1 Tiilbar if main windiw...................................................................................................28

2.2 Funotins..........................................................................................................................29

June 2, 2021 – Rev. 1 3DHISTECH Ltd. 2(76)

Page 3: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide

Disclaimer

Ci yright © 2001-2021 3DHISTECH Ltd.All Rights Reserved

Intelleotual ri erty related ti the 3DHISTECH riduots are as filliws:

EP 1,789,831; US 7,663,078; EP 2,024,772; JP 2009-512685; US 12/301,781; EP 1,644,767; JP 2007-516451; US7,327,515; EP 1,994,398; JP 2008-557835; US 12/281,566; U0700210; US 6,246,785; HU0900142; HU0700404; PCT/HU2007/000065; EP 08762668.5; US 12/663,537; PCT/AT2006/000492; JP 2008-542552; EP 06817469; US 12/095,545; PCT/AT2006/000493; EP 06817470.5; US 12/095,596; HU0900741; US 61/264,732; US 11/826,752; P0401870; PCT/IB2005/050344; US 11/662,976; HU0700409; US 12/042,050; US 77/636,144 and 007502032.

The abive mentined granted atents, granted utlity midel, registered trademarks, endinginternatinal and natinal atent a lioatins and the ither oirres inding IP are the sile ri ertesif the 3DHISTECH Ltd.

3DHISTECH Ltd. is not liable for damage of whatever nature (including, but not limited to, general or specific damage, indirect damage, consequental damage or incidental damage, including the results of the analysis of the digitied slides, for example, change of health status related to erroneous diagnosis from the digitied slide(s)) that stems from or is associated with use of Product, digitied slides, quality of staining, quality of stained slides, quality of used method of staining. 3DHISTECH Ltd. assumes no responsibility for the functonality and fault-free conditon of your “applicaton programs” (Workfows, VVA macros, Commander scripts).

Third-Party Disclaimer

THE SOFTWARE IS PROVIDED “AS IS” AND WITHOUT WARRANTY OF ANY KIND, EXPRESSED, IMPLIED OR OTHERWISE, INCLUDING WITHOUT LIMITATION, ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.

Further Informaton

Fir the latest infirmatin in 3DHISTECH riduots and servioes, lease visit iur website at: htt ://www.3dhisteoh.oim .

3 Öv StreetBuda est1141 – HUNGARY

June 2, 2021 – Rev. 1 3DHISTECH Ltd. 3(76)

Page 4: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide

Declaraton of Conformity

3DHISTECH Ltd. deolares that the riduot SlideViewer sifware, as a general in vitro medioal devioe, is subjeot ti the 8/2003. (III. 13) EszCsM (Ministry if Health, Sioial and Family Afairs) regulatin issued by the Hungarian Ministry if Health registering the 98/79/EC direotve in in vitro medioal devioes.

Further infirmatin may be ibtained frim the manufaoturer:

3DHISTECH Ltd.3 Öv Street1141 Buda est – HUNGARY

June 2, 2021 – Rev. 1 3DHISTECH Ltd. 4(76)

Page 5: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide

Character Formats and Symbols

Example Wirds ir oharaoters that a ear in the soreen. These inolude feld names, soreen and windiw ttles, ush-buttin and menu names, aths ir i tins.

Keys in the keybiard. Fir exam le, funotin keys (suoh as F11) ir the Ctrl+M key oimbinatin.

Example Criss-referenoes within this dioument ir ti ither diouments.

Warning! It might oause irreversible damage ir harm ti the user if the riduot ir the riduot itself if this instruotin is nit filliwed.

Important! It might oause the riduot ti nit wirk ri erly if this instruotin is nit filliwed and alerts ti a itentally oritoal situatin.

Note: Calls attentin ti sime im irtant infirmatin ir feature.

June 2, 2021 – Rev. 1 3DHISTECH Ltd. 5(76)

Page 6: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 1 Installatin if SlideViewer

1 Installaton of SlideViewer

1.1 System requirements

Minimum system requirements

Monitor resoluton 1320 × 720

Operaton system Windiws 7 SP1 ir newer

Graphics Card O enGL 2.0 oim atble *

CPU Intel DualCire

RAM 4GB

Disk space 750MB

* Diwnliad and install driver fir the s eoifo oard

1.2 Installaton procedure

1. Launoh the installatin fle (.exe) ti install SlideViewer in yiur oim uter.

An i tinal silent installatin mide is available if yiu want ti install the sifware based in the default setngs:

◦ Press Win+R ti i en Run windiw, then ty e cmd in the Open: feld.

◦◦ Cliok OK.

◦ Ty e the name if the installatin fle (.exe) and add a /S switoh in the oimmand line.

◦ Hit Enter ti run installer in silent mide – installatin runs in the baokgriund.

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Page 7: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 1.2 Installatin rioedure

2. If running the installer in nirmal mide (withiut adding switohes), filliw instruotins if the installatin wizard as detailed beliw:

3. If a reviius versiin if SlideViewer is installed in the oim uter, it will be u graded. Cliok Next ti ointnue.

Note:

This windiw is dis layed inly when u grading a reviius versiin if SlideViewer. In the event if u grading, make sure that SlideDriver Redistributable aokage is installed.

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Page 8: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 1.2 Installatin rioedure

4. First, aooe t terms if the Lioense Agreement, then oliok Next.

5. The installer oheoks the resenoe if rerequisite oim inents. If any if the neoessary rerequisites are nit yet installed, oliok Install ti erfirm this aotin.

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Page 9: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 1.2 Installatin rioedure

6. Onoe the neoessary rerequisites are installed, oliok Next ti ointnue with the installatin.

7. Set lioense mide. Ti Actvate with license key, seleot this i tin. Seleot Free features only tiinstall SlideViewer with features inly that are nit biund ti lioensing – exoe t TMA Funotin.

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Page 10: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 1.2 Installatin rioedure

Cliok More optons either ti Install with HASP dongle ir ti Select a license file for the purchased module.

Afer setng lioense mide, oliok Next.

June 2, 2021 – Rev. 1 3DHISTECH Ltd. 10(76)

Page 11: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 1.2 Installatin rioedure

8. The 3DHISTECH Actvaton windiw is dis layed.

Fir the inline aotvatin if SlideViewer, seleot Online actvaton (Recommended).

Fir the ifine aotvatin if SlideViewer, seleot Offline actvaton (A device with Internet connecton is stll required).

Cliok Next ti ointnue.

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Page 12: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 1.2 Installatin rioedure

9. The Actvaton form is dis layed.

10.

Important!

It is issible ti install SlideViewer withiut aotvatin. Hiwever, aotvatin is ifered ti the user at eaoh sifware start and the features related ti the dingle and the lioense fle will nit be available.

Ti fnish the aotvatin rioedure, filliw the ste s in the aotvatin firm.

• If yiu seleoted Actvate with license key and have an aotvatin key, enter it ti the key text feld if the aotvatin firm.

• If yiu seleoted Free features only, Install with HASP dongle ir Select a license file for the purchased module, leave the key text feld if the aotvatin firm em ty.

11. If the lioense is aooe ted, oliok Install ti launoh the installatin if the seleoted oim inent(s).

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Page 13: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 1.2 Installatin rioedure

12. Set destnatin filder fir the rigram fles. Cliok Vrowse ti lioate a diferent filder than the default, then oliok Next if the lioatin is defned.

13. Leave the default ir s eoify a diferent Start Menu filder ti oreate the rigram's shirtouts, then oliok Next.

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Page 14: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 1.2 Installatin rioedure

14. Tiok the twi i tins if yiu want ti Register SlideViewer as the default applicaton for opening slides and Install SlideDriver USV driver if yiu want ti use yiur SlideDriver, then oliok Install.

15. The rigress if the installatin is dis layed.

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Page 15: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 1.2 Installatin rioedure

16. Cliok Finish ti fnalize the installatin rioedure if SlideViewer.

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SlideViewer 2.5 User's Guide 2 Using SlideViewer

2 Using SlideViewer

Important!

It is the res insibility if the data iwner as i eratir ti riteot the integrity if the slide and revent unauthirized aooess. There is ni suoh riteotin within SlideViewer sifware. In this regard, the SlideCenter is reoimmended, in whioh bith are silved.

Important!

Nine if the funotins if SlideViewer oan be oinsidered as a ( reoise, oalibrated) measuring instrument. Fir exam le, by measuring the distanoe ir data ibtained by annitatin are fir infirmatin ur ises inly, the aoouraoy if whioh has nit been intentinally determined.

Important!

By enabling the Optmiiaton for Slow Connecton funotin, there may be a subjeotvely eroe tble deteriiratin in image quality due ti JPEGoim ressiin (usually very minimal, maximum deteoted relatve ti the iriginal).

Important!

All the funotinalites if SlideViewer where SlideCenter oinneotin is required are oim atble with the SlideCenter sifware, as well.

Important!

Sime s eoifoatins if nin-MRXS image firmats may nit be available fir 3DHISTECH. This may lead ti limited usability if oertain funotinalites.

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Page 17: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 2 Using SlideViewer

1. Diuble-oliok the rigram shirtout ioin in yiur deskti ti dis lay the startu windiw if the a lioatin.

If yiur system dies nit su irt O enGL hardware aooeleratin (either nit having a ri er videi oard ir driver installed), the beliw message is reoeived. Cliok OK ir Do not show again ti launoh SlideViewer.

Note:

SlideViewer is i eratable withiut O enGL, but will wirk sliwer.

2. In the startu windiw if SlideViewer, seleot the desired stirage yiu want ti aooess:

Local Computer

In the lef ane if the windiw, the tree struoture if the seleoted drive oan be ex anded. If yiu oliok in a filder the thumbnails and label areas if the inoluded slides are dis layed in the right ane if the windiw. Cliok the slide thumbnail ti i en the slide in SlideViewer.

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SlideViewer 2.5 User's Guide 2 Using SlideViewer

In the tree struoture if the seleoted drive bith the name and the image firmat if the slide are dis layed.

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Page 19: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 2 Using SlideViewer

Note:

A erii, DICOM, and Hamamatsu slides oan be i ened as well in SlideViewer, but with limited funotinality (yiu oannit annitate areas ir u liad slides ti SlideCenter server, eto.)

Note:

If a read-inly slide is i ened either frim the lioal stirage ir SlideCenter server, the “[Read inly]” text is added afer the slide name in the main windiw.

• – u dates the tree view if the seleoted filder (it is advised ti oliok this buttin afer any midifoatin is dine in the filder struoture).

• – feld fir searoh slides ir filders in the tree, furthermire, adds UNC ath ti the tree.

• – oliok this buttin ti add the seleoted filder ti Favirites. The marked filders arelisted under the Favirites filder at the ti if the tree-view anel.

• – the Upload/Processing anel serves ti oilleot slides fir u liad ti SlideCenter ir fir batoh rioessing.

First, seleot the slide ir a single filder in the tree-view, then oliok ti add it ti thelist. Yiu oan remive a slide frim the list by olioking . If yiu want ti delete all items, oliok .

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SlideViewer 2.5 User's Guide 2 Using SlideViewer

• – Export Slide

Thriugh this funotin the Slide Cinverter rigram is i ened with whioh a slide (ir slides) ir its s eoifo areas oan be ex irted ti diferent fle firmats (MRXS, Riohe Tif, Tiled Tif, SVS). Fir mire infirmatin in the use if Slide Cinverter, lease refer ti Slide Converter 2.4.1 User's Guide.

• – rioesses seleoted slide(s) with Image Analysis Modules.

• Batoh u liad if slides ti SlideCenter server oan be initated by olioking .

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Page 21: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 2 Using SlideViewer

a) If yiu already are ligged in ti the server, the tree-view if the server is dis layed. First seleot a filder as destnatin, then oliok Start upload. Ti oinneot ti anither server as destnatin, oliok at the header bar.

b) If yiu are nit yet oinneoted ti any servers, oliok the buttin if the redefned server ti whioh yiu oan oinneot ti, ir oreate a new oinneotin. Afer ligging in ti a server, the tree-view is dis layed.

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Page 22: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 2 Using SlideViewer

• Afer olioking Start Upload, the Slide Prioess Queue windiw is dis layed in whioh the u liad rigress if slides is visible.

• Cliok ti dis lay the anel inoluding the list if slides suooessfully u liaded in theHistiry anel.

▪ Cliok ti dis lay slide infirmatin in the siuroe and destnatin. By olioking all items oan remived frim the queue. Nite, that an item that is being rioessed oannit be deleted this way. Cliok ti delete the s eoifo item frim the queue.

▪ If the u liad rioess is interru ted ir oiuld nit be erfirmed, the slide name is visible in red, and gets ti the bittim if the list. Cliok ti retry u liad. In the infi anel the oause if the errir is dis layed.

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SlideViewer 2.5 User's Guide 2 Using SlideViewer

SlideCenter server

• Cliok the menu bar buttin if the SlideCenter server oinneotin ti i en the ligin windiw ti oinneot ti the dedioated server, then the ligin windiw if the seleoted server is dis layed if yiu are nit already oinneoted.

• Enter user name and asswird, then oliok Login ti oinneot, lioate the slide in the SlideCenter server, then oliok its thumbnail ti i en in SlideViewer.

• If the server yiu want ti u liad slides ti is nit listed, oliok ti defne the oinneotin ti anither SlideCenter server.

Enter Server Name and Server Address, and aotvate Secure connecton if the SlideCenter server yiu want ti oinneot ti uses an HTTPS ritioil. If SlideCenter server i tin is disabled, any website oan be i ened. Cliok OK when fnished.

Note:

The maximum number if defnable servers is 8.

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Page 24: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 2 Using SlideViewer

Cliok ti midify server name, address, and/ir oinneotin ty e.

Cliok ti the right if the SlideCenter server oinneotin buttin, then oliok Yes in the message windiw ti delete the server.

Ti i en a slide that is inoluded in a Teleoinsultatin sessiin, and attend teleoinsultatin via SlideViewer, di the filliwing:

1. Cliok the SlideCenter server oinneotin buttin in the startu windiw.

2. Ligin ti SlideCenter.

3. Gi ti Teleconsultaton windiw, TC requests anel

4. Seleot the TC sessiin yiu are invited ti.

5. Cliok ti jiin the sessiin.

6. Enter User Name in the TELECONSULTATION windiw (oan be diferent than the SlideCenter user name), then oliok OK.

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Page 25: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 2 Using SlideViewer

7. The frst slide seleoted fir the Teleoinsultatin sessiin is dis layed in the SlideViewer windiw. A ointril anel with additinal buttins related ti teleoinsultatin funotins are dis layed at the ti lef oirner.

• If yiu are the hist if the sessiin, the buttin is aotve (as seen abive), itherwise yiu oan request fir slide ointril by olioking the

buttin. Ti turn slide mivement in/if, thus making it visible ir hidden fir guests, oliok the buttin.

Note:

The hist must a rive the request, and the ointril oan be taken baok any tme the hist wants ti.

• Ti quit sessiin, oliok . The SlideCenter server windiw turns aotve again.

• Ti i en a ohat windiw, oliok .

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SlideViewer 2.5 User's Guide 2 Using SlideViewer

• Ty e yiur message (visible ti all the artoi ants if the TC sessiin) inti the feld at the bittim, then oliok .

Note:

The Teleoinsultatin ohat windiw oan mived by dragging at the windiw header, and oan be resized by dragging the bittim right oirner.

The anes if the ohat windiw ( artoi ants list at the lef, message feld, and ohat ane) oan be resized by miving the draggable divider bar. The artoi ants list oan be olised ir ex anded by olioking the arriw buttin at the middle if the vertoal divider bar.

The name if the ointriller artoi ant is highlighted, and the start tme ir tme frame (if the tme when a user has lef the sessiin is s eoifed) is visible in the braokets. The start tme is s eoifed by the tme assed sinoe i ening the sessiin.

The name if the artoi ant that has lef the sessiin is grayed iut, and an end-tme is s eoifed in the tme-frame braoket. When this user jiins the sessiin again, its name is added ti the artoi ants list in a new line.

The request fir ointril sent by the requester artoi ant is reoeived by the ointriller user. Anyine whi has the ointril oan ass the ointril iver ti the requester, but the hist if the sessiin is alliwed ti take iver the ointril at any tme. If the hist jiins the sessiin, the ointril will nit be autimatoally given ti him/her. The hist oan take iver the ointril by olioking the buttin.

If a message is sent by anither artoi ant, and the ohat windiw is olised, a message bubble ioin a ears at the bittim if the Teleoinsultatin ointril anel.

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Page 27: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 2 Using SlideViewer

If the hist olioks the buttin ti disoinneot, a windiw a ears in whioh a ohat lig oan be saved ti a CSV fle ti the seleoted filder. The filliwing data are saved in the CSV fle:

• Teleoinsultatin ttle

• Name if hist user

• Start tme (TC sessiin)

• End tme (TC sessiin)

• The tme when the user has jiined sinoe launohing the sessiin (and rejiining tme as well if rejiined)

• Aotvites with tme stam s: ointril events, slide i ening events, griu iwner ohanges, ohat messages (tme stam based in the tme assed sinoe sessiin launoh)

• Griu iwner ohanges: when twi artoi ants are already attending the sessiin and the hist jiins, the hist beoimes the new griu iwner. If there are inly users as artoi ants in the sessiin, the griu iwner is the user whi has jiined frst.

Important!

If anyine aming the artoi ants whi has jiined the Teleoinsultatin sessiin i erates a reviius versiin if SlideViewer (2.3 ir earlier), all ifthe sessiin artoi ants reoeive a warning message that these users oan i erate inly with limited funotinality.

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Page 28: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 2.1 Tiilbar if main windiw

2.1 Toolbar of main window

Afer seleotng the slide ti i en, it will be autimatoally dis layed in SlideViewer. SlideViewer ifers variius funotins in a well-arranged user interfaoe.

The funotins aooessible by olioking the highlighted tiilbar buttins are desoribed in detail in section 2.2.

Note:

If yiu ress F11 ti aotvate full soreen, the nirmal windiw oan be restired by olioking .

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Page 29: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 2.2 Funotins

2.2 Functons

1. Open/Close Slide browser

Switoh ti lioal tree-view ir seleot anither filder within the server yiu are oinneoted ti ir in anither server.

2. Slide operatons

U in seleotng this funotin, fve i tins are available fir yiu ti seleot:

• – Diwnliad slide ti lioal filder (if the slide is i ened frim SlideCenter) OR

– U liad slide ti SlideCenter (if a lioal slide is i ened)

See section 2 fir further infirmatin in u liad ti SlideCenter.

• – Slide informaton

Slide Descripton and/ir Slide Informaton windiws are dis layed ointaining slide-related infirmatin.

Note:

Slide desori tin is available inly fir slides i ened frim SlideCenter.

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Page 30: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 2.2 Funotins

Cliok ti oi y infirmatin ti oli biard.

• – Pixel informaton

Slide oiirdinates and oilir intensity values er ohannel are dis layed in this anel. Pixel inter ilatin is turned if when this anel is i en, therefire ixel biundaries are olearly visible. Turn if Zoom Limit funotin ti ins eot ixels at the highest magnifoatin if neoessary.

When i ening image fles, the ixel density value oan be midifed afer olioking the buttin beliw the soale bar. Set a new value in the Change pixel density (µm/px) windiw, and oliok OK. If the resilutin if an .mrxs slide is higher than 2 µm/ x, the oilir if the soale bar turns ti red and the value oan be midifed manually. Afer any midifoatin was made ti the µm/ x area, the text will be dis layed with blue.

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SlideViewer 2.5 User's Guide 2.2 Funotins

• – Export Slide

Thriugh this funotin the Slide Cinverter rigram is i ened with whioh a slide (ir slides) ir its s eoifo areas oan be ex irted ti diferent fle firmats (MRXS, Riohe Tif, Tiled Tif, SVS). Fir mire infirmatin the use if Slide Cinverter, lease refer ti Slide Converter 2.4.1 User's Guide.

3. Slide overview

Shiws a liw resilutin iverview frim the while slide ti hel navigatin. The soreened area is marked with a reotangle with a birder ir with a oriss de ending in the ourrent ziim level. It alliws fast navigatin thriugh the slide by olioking inti a oertain iint in the iverview ir anning with the reotangle.

Mive windiw by dragging it by the buttin at the ti lef oirner. Drag ti enlarge windiw.

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Page 32: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 2.2 Funotins

Ti turn in Tracking History funotin, oliok at the bittim lef oirner if the windiw. This enables fir yiu ti reoird the traok if slide areas yiu have ins eoted.

Traoking ma oilir oan be ohanged by olioking . Seleot ir edit a oilir fir the ma .

The reoirded histiry oan be deleted by olioking in the Traoking Histiry anel. Cliok Yes in the a earing windiw ti oinfrm deletin, itherwise oliok No.

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Page 33: SlideViewer 2.5 User's Guide

SlideViewer 2.5 User's Guide 2.2 Funotins

4. Magnifier

Magnifes a oertain area if the slide. The magnifer windiw oan be dragged and dri ed in the slide.The area beneath the magnifer windiw is magnifed and dis layed in this windiw. By olioking the buttin the area ariund the oursir iinter is dis layed in the magnifer windiw. Cliok ti restire default view in whioh either the slide ir the magnifer windiw oan be dragged ti dis lay magnifed area if the slide.

Detached Magnifer

Pinned Magnifer

Magnifer windiw size oan be ohanged by dragging at its right bittim oirner. Magnifoatin rate within the magnifer windiw oan be midifed by sorilling with the miuse wheel. The limits if this magnifoatin oan be set in the settings windiw.

5. Label area

Shiws the label area if the slide. With the ritatin ioin the label image oan be fi ed by 180°. Label area windiw oan be re-sized by dragging the ioin at the bittim lef oirner. Slide Descripton and/ir Slide Informaton windiws oan be dis layed by olioking the ioin. See funotin 2. Slide operations in this seotin fir mire infirmatin.

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Cliok at the bittim right oirner if the label area windiw ti i en the Soan Preview image if the slide.

Cliok at the bittim right oirner if the label area windiw ti i en the Whileslide Soan Preview (Soan Preview + Label area) image if the slide.

Important!

Slides soanned with earlier soanner versiins may nit inolude Whileslide Soan Preview and in this oase, by olioking , an em ty slide image is dis layed.

The review image oan be ziimed in/iut by sorilling, and mived in the windiw with the miuse. Thewindiw oan be resized by dragging at the oirner.

• Cliok and hild ti detaoh and drag the windiw.

• Cliok ti save review image as JPG, PNG, BMP, ir TIFF fle ti the seleoted filder in the i -u windiw.

• Cliok ti olise the Soan Preview windiw.

During slide oinversiin in Slide Cinverter, the slide oan be aninymized by seleotng the Anonymiie slide funotin, thus remiving baroide (identfoatin) infirmatin and making label area unreadable.

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The aninymized label if the slide is dis layed as shiwn beliw:

6. Pan mode

Use this mide ti navigate in the slide.

7. Annotaton mode

This mide alliws laoement if several diferent kind if annitatins in the slide, suoh as:

• Free size reotangle ( ress and hild SHIFT key while resizing ti maintain as eot rati)

• Free oirole ( ress and hild SHIFT key while resizing ti maintain as eot rati)

• Measurement annitatin

• Free hand linear

• Free hand area

• Arriw-ty e annitatin: draws an arriw ti a iint if interest in the slide.

• Soreened: annitates the area visible in the minitir. This funotin is a built in funotin if SlideDriver as well.

• Fix size reotangle: draws a fxed size reotangle annitatin in the slide. The annitatin size oan be set u in the settings windiw.

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• Fix size oiroular: draws a fxed size oiroular annitatin in the slide. The annitatin size oan be set u in the settings windiw.

• 20 × FOV: uts a standard 20 × feld if view sized annitatin in the slide. The annitatinsize oan be midifed in the settings windiw.

• 40 × FOV: uts a standard 40 × feld if view sized annitatin in the slide. The annitatinsize oan be midifed in the settings windiw.

It is alsi issible ti ritate annitatins, where it is needed and makes sense. Ti seleot an annitatin, oliok lef miuse buttin in the annitatin label. Ti seleot fir editng, oliok right miuse buttin in the annitatin label. Ti ritate the annitatin, oliok lef miuse buttin and drag the arriw in the ti if the annitatin.

Ti save the ohanges, oliok right miuse buttin in the annitatin label ir anywhere in the slide.

• Plaoes TMA markers in the slide: seleot the marker buttin if the desired diameter frim the anel, then laoe markers in the slide. Cliok ti midify default oilirs if the seleoted marker.

•Afer yiu have seleoted the diameter, kee the lef miuse buttin ressed si that the em laoement if marker is easier as it is dragged aling with the mivement if the miuse. Release lef miuse buttin ti laoe the marker. If mis laoed, right-oliok the belt area if the marker, then drag it by the arriws at its oenter iint by kee ing the lef miuse buttin ressed.

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If yiu want ti delete a marker, right-oliok its belt area, then hit the Delete key. Cinfrm deletin by olioking Yes in the a earing message bix.

• Annitatin sohema griu s: with the hel if the annitatin sohema griu s, yiu oan easily diferentate areas if the slide within ine griu if annitatins and make them oinsistent fir a s eoifo gial as illustrated in the fgure beliw.

Cliok this ioin ti oreate Annotaton schema groups and the list if annitatin griu s is dis layed.

Seleot annitatin griu ti wirk with frim the dri -diwn menu. Ti draw re-defned annitatins, oliok the intended oilir bix then oliok in the slide image where yiu want ti laoe the annitatin.

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Ti set annitatin soales fir fx-size annitatins, see Annotaton scales in Settings.

Cliok the buttin ti dis lay the Annotaton groups anel and list annitatin sohema griu s.

◦ Cliok the buttin ti add a new sohema griu . In a i -u windiw, enter the Schema Group Name. Cliok OK ti add the new sohema griu ti the list.

◦ Cliok ti rename the seleoted sohema griu in a i -u windiw.

◦ Cliok ti delete the seleoted sohema griu .

◦ Cliok ti dis lay the Schema editor windiw and add annitatin ty e ti the griu .

▪ In the Schema Editor windiw, enter Schema name and oliok the buttin ti defnesohema oilir. There are 8 redefned, and 4 editable oilir buttins available fir yiu ti seleot.

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Cliok the oilired bix ti seleot sohema oilir and oliok ti a ly the seleotin.

▪ Seleot the Auto naming and Auto numbering seleotin bixes if yiu want ti add yiur sohema the re-defned values entered in the text bix beliw the annitatin ty e dri -diwn menu.

▪ Seleot annitatin ty e in the dri -diwn menu. If yiu want ti, enter Annotaton default note.

▪ Cliok ti save ohanges.

• Dis lays Annotatons anel in whioh the oreated annitatins and their related infirmatin are listed. Mive Annotatons anel by dragging it (olioking in the anel header).

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Note:

Annitatins anel is dis layed autimatoally and all the annitatins will be seleoted bith in the slide and the anel whenever yiu ress CTRL+A.

The filliwing funotins are available in this anel:

• Drag annitatins ti reirder them by grabbing at the lef side if annitatin name.

• Edit annotaton: oliok this buttin ti ohange the annitatin name.

• Change annotaton color: oliok this buttin ti seleot ir edit a oilir fir the annitatin frim the Defne Cilir anel. There are 8 redefned, and 4 editable oilir buttins available fir yiu ti seleot.

• If the slide is i ened frim SlideCenter, by olioking this buttin a link fir the s eoifo annitatin in the slide is oi ied ti the oli biard.

When insertng this link as hy erlink in a text editir, the slide will be i ened and the annitatin is ziimed in afer olioking the link.

• Click for note – Field fir annitatin desori tin. Entry if mult le data and oi y/out/ aste funotin is su irted.

• Export selected annotatons – see section 2.2 – 2. Slide operations fir mire infirmatin.

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• Defne area for re-scan: a bitma image is oreated that oan be used by the ointril sifware if soanners – re-soanning if seleoted areas based in the soanma is available if neoessary.

• Export data to CSV: ex irts annitatin ri ertes, e.g. name, area, and erimeter if the seleoted annitatin inti a CSV fle.

• Create Virtual Tray or Replicated Slide: If inly ine annitatin is seleoted, then Virtual Slide ir Virtual Tray oan be oreated. If mire annitatins are seleoted, inly Virtual Tray oan be oreated.

Note:

This funotin is available inly if the slide is i ened frim SlideCenter.

Note:

It is available fir yiu ti add an annitatin ti a VT as an additinal VT item in mult-view mide.

• Clone selected annotaton: this funotin alliws the du lioatin if the seleoted annitatin frim ine slide ti the ither in MultView mide.

• Annotaton history: this funotin alliws the user nit inly ti list annitatin events but restire earlier deleted annitatins.

Cliok this buttin in the Annotatons windiw ti dis lay Annotaton history.

Seleot an annitatin event frim the list and oliok . The annitatin related ti that event is restired bith in the slide and the annitatin list (with blue letters).

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Cliok ti switoh between UTC and lioal tme zines. Seleot number if histiry

events ti liad inti the list in the dri -diwn menu and oliok the ioin ti liad.

Cliok ti oheok new histiry items.

Note:

Lioal users are dis layed as unkniwn in the annitatin histiry but the user name is dis layed when ligged in ti the server.

Note:

While restire histiry funotin is aotve and the buttin is marked, editng annitatins is nit alliwed.

• Delete: deletes the seleoted annitatin(s).

• By olioking lef ir right arriws the reviius ir the next annitatin oan be dis layed in the oenter if the windiw.

• View / Hide all annotatons

• View / Hide selected annotaton

Note:

Annitatin sizes afer being drawn may slightly difer frim the sizes if the same annitatins afer rei ening the slide(s) ir SlideViewer. The main oause if this henimenin is that the sifware saves annitatins ti slides with riunded values aooirding ti slide oiirdinates. This is alsiavailable fir reotangular annitatins as well, in a degree de ending in slide resilutin.

The Annotatons anel is nit dis layed autimatoally afer oreatng an annitatin, alliwing yiu ti ut annitatins ointnuiusly in the slide.

Afer olioking the inned mide is switohed if, dis layed as , si the Annotatons anel is i ened right afer yiu annitate an area, enabling yiu ti set ir midify the annitatin yiu oreated.

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8. Flip or rotate slide

Switohing ti fi -ir-ritate mide, the slide oan be fi ed either hirizintally ir vertoally and alsi oan be ritated using the slider, 0-180° either oliokwise ir oiunter-oliokwise.

The (reset ritatin) funotin ritates the slide baok ti its iriginal isitin (as it was in the glass

slide). The ( in) funotin fxes the anel, meaning that the anel remains i en during i eratins.If the anel is nit inned, it will be olised afer olioking any if the anel buttins.

9. Measure length

Create sim le length measurement by defning a startng and an end iint if measurement in the slide by olioking with the miuse. The length is dis layed in the oinneotng line.

10. Create snapshot

This feature alliws the saving ir oi ying if a soreen-shit made frim the ourrent visible area.

Show annotatons – annitated areas are shiwn in the Sna shit image

Show scale bar – a soale bar is dis layed in the Sna shit image

Show slide overview – a review image if the slide is dis layed in the Sna shit image

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Note:

Ti enable this i tin, the Slide Overview funotin must be aotvated frst.

Show slide label – slide label is dis layed

Note:

If Show slide label i tin seleoted and Scan preview is aotve, it will alsi be added ti the sna shit.

• The buttins re resent the lioatins if the abive three elements (Show scale bar, Show slide overview, and Show slide label) in the windiw, that oan be midifed by olioking the buttins.

Image resoluton – seleot ine if the filliwing:

• Screen resoluton i tin sets image resilutin identoal with the a lied resilutin if the minitir

• Print resoluton (300 dpi) sets image resilutin ti 300 d i

Cliok ti oi y image ti oli biard.

Cliok ti save image as JPG, BMP, PNG, ir TIFF fle.

If yiu want ti set sna shit image ri ertes and oreate a DICOM image, oliok .

Show annotatons – annitated areas are highlighted in the Sna shit image

Show scale bar – a soale bar is dis layed in the Sna shit image

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Show slide overview – a review image if the slide is dis layed in the Sna shit image

Show slide label – slide label is dis layed in the Sna shit image

• The buttins re resent the lioatins if the abive three elements (Show scale bar, Show slide overview, and Show slide label) in the windiw, that oan be midifed by olioking the buttins.

Cliok ti save image as a DICOM fle.

11. Zoom levels

These ointrils ohange the magnifoatin frim ft-ti-soreen ti 63×, 1:1, and arbitrary levels. The ourrent ziim level is dis layed and oan be midifed in the far right feld. iiming in/iut oan be alsi aohieved by ressing the -/+ keys, and fast ziiming is enabled when hilding SHIFT at the same tme.

Note:

Visualizatin if struotures oan be diferent de ending in the a lied ziim level. The halt in ziiming at 1:1 magnifoatin is intentinally designed as an indioatir.

12. Color channel adjustments

Cilir adjustment might be neoessary fir the best visualizatin results fir yiur dis lay. Bith anels if oilir histigram (8-bit and 16-bit) fir Brightield and Fluiresoent slides inolude adjustable brightness

, ointrast (bith in X and Y axes), and gamma levels. This menu ioin has twi states, is dis layed if default oilir setng values are liaded ir restired afer midifoatin, and is visible if values have been midifed.

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Bittim end-sliders are set ti the darkest (Blaok) and lightest (White) tines that ioour in the image. The gial fir faded images is ti mive the blaok and white iint sliders in ti ir near the darkestand lightest tines that aotually ioour in the image. The middle slider adjusts the mid-tines (Gamma) in the image withiut ohanging the blaok and white values.

Histigram range oan be ziimed/narriwed diwn by dragging and at the end- iints. By right-olioking ir diuble-olioking in arriws set values oan be restired ti default and vioe versa. The histigram if 16-bit slides oan be ziimed in with the miuse by hilding the lef miuse buttin ressedduring seleotin.

Brightield slides

Brightness, ointrast, and gamma values oan be midifed in the Color Adjust anel. Changes take efeot immediately. A oilir histigram if the slide is available in this anel ti su irt reoise adjustment.

- Current area histigram dis lays the histigram if the ourrently visible area if the slide. Aotvated autimatoally if the slide dies nit ointain a reviiusly saved histigram.

- Best ft

- Sets i tmal ointrast

- Saves setngs ti slide

- Restires setngs frim slide (if saved)

- Resets default values

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RGB ohannels oan be adjusted individually in the Color Balance anel afer olioking . Cliok ti reset values.

Fluorescent slides

Fluiresoent slides ointain individual ohannels fir eaoh fuiresoent signal. This anel re resents the available fuiresoent ohannels if the slide. Cilir adjustment if a fuiresoent slide is available ohannel by ohannel. Cliok a ohannel ti seleot and midify its ourve, brightness, ointrast, and gamma values in the histigram.

- Current area histigram dis lays the histigram if the ourrently visible area if the slide

- Linear/Ligarithmio soale in Y-axis

- Auti-fuiresoenoe fltering methid oan be a lied in FL slides by olioking this buttin

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- Best ft

- Sets i tmal ointrast

- Saves setngs ti slide

- Restires setngs frim slide (if saved)

- Resets default values

Single ohannel dis lay mide oan be aotvated u in olioking . If gray-soale mide is aotvated, inly ine seleoted ohannel is visible and midifable at a tme.

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Fir fuiresoent slides, the tiilbar fir oilir ohannel adjustments is lioated at the bittim lef oirner if the main windiw re resentng the available fuiresoent ohannels if the slide.

• Cliok a label ti turn a ohannel in ir if. Channels oan be dis layed in graysoale mide u in

olioking , and alsi yiu oan switoh between single ohannel and mult ohannel mides. If graysoale mide is aotvated, inly ine seleoted ohannel is visible at a tme.

• Cilir adjustment if a fuiresoent slide is available ohannel byohannel. Right oliok an aotve ohannel ti midify its re resentatveoilir in the Pseudo-Color anel, where yiu oan seleot ine if the8 redefned oilirs, ir adjust RGB ohannels ti oreate a uniqueoilir, and erfirm the filliwing aotins:

- Saves setngs ti slide

- Restires setngs frim slide (if saved)

- Resets default values

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13. Plugins

– Manage quantficatons with QuantCenter

The slide will be i ened in QuantCenter u in olioking this buttin. Fir mire infirmatin in quantfoatin-related funotins and features, lease, read QuantCenter User's Guide.

– Virtual Tray management panel

This buttin is aotve inly if a Virtual Tray is i ened. Cim artment Virtual Slides integrated inti the same Virtual Tray oan be managed in this anel.

The list shiws oim artment slides if a Virtual Tray. Cim artments oan be managed as layers – drag and dri the seleoted oim artment ti view in the frint ir send it ti baok, ir oliok ti hide them.

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The i aoity if the oim artment alsi oan be set with the alpha slider lioated at the right sideif the Virtual Tray anel. It is alsi available fir yiu ti delete a slide frim the list, ir rename a slide.

• Mive a virtual slide by dragging it.

• Manage Virtual Tray items:

Cliok the slide ti seleot, then it oan be deleted - , hidden - , ir its name oan

be edited - if neoessary. Mive between slides by olioking the buttins.

• O ens the anel ointaining baokgriund tiils. The filliwing funotins are available:

◦ Seleots baokgriund oilir and make it trans arent.

◦ Shiws/Hides baokgriund.

◦ Resets baokgriund.

◦ Undi-Redi

– Digital Diferental Interference Contrast (DDIC)

This funotin is useful ti enhanoe the ointrast in almist trans arent ir faded sam les. DDIC wirks in the rinoi le if interferimetry ti gain infirmatin abiut the i toal ath length if the sam le, ti see itherwise hardly visible tssue struoture features. The image has the a earanoe if a three-dimensiinal ibjeot under very iblique illuminatin, oausing string light and dark shadiws in the oirres inding faoes.

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When olioking the right seotin if the buttin if DDIC is aotvated, the gray-soale image if the slide is visible. A relatvely oim lex lightng soheme riduoes an image with the ibjeot a earing blaok ti white in a gray baokgriund.

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– Gradient map visualiiaton

With this funotin the oritoal areas if the slide oan be highlighted.

When yiu aotvate this funotin fir Brightield slides, the stain-based isitvity is visualized:

Fir Fluiresoent slides the intensity if the gray-soale image is highlighted:

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Based in a redefned oriteria, the filliwing oilirs are used fir the image ti visualize diferent areas if the slide:

• Vlue: insignifoant

• Green: simewhat signifoant

• Yellow: signifoant

• Orange: mire signifoant

• Red: mist signifoant

– Marker Counter

U in olioking this buttin the filliwing anel a ears thriugh whioh marker-related funotins are available fir yiu ti seleot:

• – Shiws a list if markers: saved markers are listed in the liwer anel (Available Categories), used markers in the slide are listed in the u er anel (UsedCategories).

Seleot a marker frim the list if Available Categories, then oliok ti add the marker ti the list if Used Categories.

The marker oategiry added ti the list is then resented by a buttin in the marker anel at the lef side if the SlideViewer windiw. If yiu have mire than fiur oategiries in the anel, yiu oan sorill diwn ti seleot a nin-visible oategiry yiu wish ti use by olioking .

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• A new oategiry oan be oreated by olioking the buttin.

• Ty e oategiry name inti the text feld, set its oilir by olioking then seleotng the desired oilir frim the redefned alette ir adjust RGB ohannels ti give it a unique oilir.

• Set its ty e – Filled ir Outline, its Diameter (µm) and Line width (px). Aotvate Add to Used ti add the newly oreated marker ti the list if Used Categories.

Cliok ti save the new marker oategiry.

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Important!

It is nit issible ti oreate mire than ine marker oategiries with identoal names.

• Cliok ti edit marker oategiry setngs suoh as name, oilir, ty e, diameter, and line width.

Note:

Any midifoatin ti the oategiry will be a lied ti all if the markers if

the same kind afer olioking .

• Ti delete a marker oategiry, frst seleot it, then oliok . Cliok Yes in the message bixti oinfrm deletin.

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Add markers ti the slide by seleotng the marker oategiry frim the anel frst, then mark oategiry-related s its by olioking with the lef miuse buttin.

• – Cliok this buttin ti delete unneoessary markers by olioking either with the lef- ir right-miuse-buttin. If the deletin funotin is nit aotvated, markers oan be deleted by olioking with the right-miuse-buttin inly.

• – O ens the Marker Statstcs anel. First, seleot the annitatin frim the dri -diwn list, then the related statstoal data will be shiwn. Switoh between µm2 and mm2 ROI surfaoe area measurement units with the buttin.

• – Cliok this buttin ti delete all if the markers if the seleoted oategiry laoed within the annitatin.

• – Ci ies table ti oli biard

• – Ex irts table ti CSV fle

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– TMA Functon

U in olioking this buttin an autimated TMA oire deteotin is erfirmed and the TMA Funotin windiw is dis layed in whioh the funotin-related setngs are dis layed.

Results oan be fltered by the filliwing i tins:

• – O en TMA database fle generated by a TMA ointril sifware. Afer olioking this buttin, lioate the TMA database fle in the briwser windiw (this is i tinal, the funotin oan be used alsi withiut defning a database).

The Recipient Vlocks dri -diwn list is visible inly if a database has been liaded. In this list the reoi ient bliok data frim the XLS/XLSX ir ODS fle are dis layed. Onoe yiu seleot a reoi ient bliok the bliok-related oire data are listed.

Important!

The handling if XLSX fles ex irted frim LibreOfoe ir O enOfoe are nit su irted.

• The filliwing data are dis layed inoe the s it reoignitin is erfirmed:

• Cire names (dis layed abive the markers)

• Cire-related bliok/seotir data

• Cire-related atent data (if database has been liaded)

• Deteoted size (diameter) if oires

• Cire isitins

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Cols and Rows re resent number if riws/oilumns ti be lef iut frim the tital grid, X and Y values defne the isitins frim whioh the grid fragment is shiwn.

1. Fir exam le, frst, deorease the Row ir Column value.

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2. Inorease the X ir Y value, and the filliwing grid seotin aooirding ti the midifed axis is shiwn in the slide image.

Cols, Rows: these felds dis lay the number if riws/oilumns deteoted by the algirithm

Orientaton: the ti lef oirner is the default irientatin if the grid.

Flip: tok this oheokbix ti fi the irientatin if the grid.

Core Siie: deteoted oire-size

Intensity threshold: Cire intensity value is 0 by default.

Show captons: Cire oa tins are shiwn by default, but they oan be turned if when this i tin is inaotve.

Create annotatons: if all the arameters are oinfgured ri erly, oliok ti a ly annitatins ti the slide.

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– 3D Peek

This funotin is biund ti the 3D Peek lioense, and is a new feature if SlideViewer, by whioh -Staok slides oan be resented in 3D, thus rividing a windiw inti the investgatin if tssue struotures in 3D. This tiil is i tmal fir the 3D visualizatin if brightield and fuiresoent slides as well.

– Start/Abirt 3D reoinstruotin

– Seleot sim le (ROI within the white marker reotangle) area ir annitatin as in ut

– High-Density visualizatin

– Stretoh vertoally

– Shiw grid

– Ritate animatin

• - Deorease animatin s eed

• - Inorease animatin s eed

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• - Ritate frim lef ti right

• - Ritate frim right ti lef

– Take sna shit

– Reoird videi

• A videi fle is oreated in AVI firmat if the animatin ir manual i eratin if the seleoted area ir annitatin. The aotual length in mm:ss firmat is dis layed at the bittim right oirner if the visualizatin windiw.

• Cliok ti sti reoirding. The default filder set in Settings/Utlity settings/3D Peek video folder is i ened. The name if the oreated videi fle oinsist if the slide name, magnifoatin rati, and date/tme stam attaohed at the end (fir exam le: DigitalSlide01_29.7x_20-04-28_19-16-05.088). If the oim lete videi is bigger than 2.5GB, it will be s lit inti se arate arts (2.5GB units).

The seleoted area oan be dragged, ritated and ziimed in/iut with the miuse (lef, right, and sorill buttins res eotvely).

The visualizatin windiw oan be reduoed ti small-view ir restired ti the reviiusly set size by olioking . Mive windiw by dragging it at the area if the windiw. Midify windiw size by dragging the areas at the bittim oirners. Cliok ti olise the windiw.

14. Official website

U in olioking this area the website if 3DHISTECH Ltd. (htt ://www.3dhisteoh.oim/) is i ened in yiurbriwser.

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15. Settings & Help

The frst fiur sub-menus ointain i eratin- and sifware-related guidanoe, hel in hiw ti use the tiuohsoreen, hitkeys, and diwnliad the PDF firmat if this manual.

– Pathology guidelines

U in olioking this buttin variius athiligy guidelines oan be dis layed as aids fir soiring.

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– Open Keyboard Shortcuts help

This windiw inoludes the filliwing anels:

• View & 3D Peek

This anel ointains keybiard shirtouts yiu oan use in SlideViewer ti aotvate View and3D Peek funotins.

• Slides, Annotatons & Trays

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• Other shortcuts

• General Touch Events

This windiw inoludes infirmatin regarding i eratng the sifware thriugh the tiuohsoreen if yiur devioe.

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• 3D Peek Touch Events

This windiw inoludes infirmatin regarding i eratng the sifware thriugh the tiuohsoreen if yiur devioe when viewing slides with the 3D Peek tiil.

– Open User Guide (PDF)

The SlideViewer User Guide in PDF firmat is i ened u in olioking this buttin.

– About this program

Cliok the buttin ti reset the aotual aotvatin. Afer the reset, when restartng Slideviewer, the aotvatin windiw is dis layed again and a new aotvatin rioedure oan be erfirmed.

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- Settings

O eratin-related setngs if SlideViewer oan be aooessed thriugh this windiw, and it inoludes the filliwing anels:

• - Main settings

• Open Slide overview with every slide: Turn in/if autimato i ening if slide iverview with the slide

• Open Label area with every slide: Turn in/if autimato i ening if label area with the slide

• Open Scan preview with slide label: Turn in/if autimato i ening if soan reviewwith the slide label.

Note:

If the autimato i ening if Label area is turned if, this funotin remains aotve if turned in, but will nit autimatoally i en with the slide).

• Open with Vest Fit coloriiaton: Slide is i ened with the Best Fit oilirizatin funotin a lied by default.

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• User Interface

◦ GUI design style: it is available fir yiu ti seleot frim twi diferent styles – Classic and Flat.

◦ Preferred GUI Scale: seleot the soale if the interfaoe within the range if 50% and 400% if neoessary. Soaling de ends in the SlideViewer windiw size and theminitir resilutin; si if fir exam le 200% is set, the aotual soaling oan be less than that.

• Default slide orientaton: if yiu want ti defne an irregular default slide irientatin,

oliok the buttin ti ritate slide by 90°, 180° ir 270°.

• The Apply ioom limit funotin restriots the a lioable maximum magnifoatin rate ti 2x if 1:1; if nit aotvated, it alliws ziiming at 16x if 1:1.

• Smooth slide navigaton: When ziiming in ir iut in the image, the transitin between ziim levels and during navigatin is erfirmed withiut abru tness.

• - Utlity settings

• Magnifying: sets magnifer windiw attributes. Seleot the i tin Relatve ti set magnifer windiw magnifoatin ti be de endent in the magnifoatin if the slide. If seleotng the i tin Absolute, the magnifer dis lays the set magnifoatin. Absolute value oan be set either ti 20x ir 40x magnifoatin by olioking the oirres inding buttin.

Cliok ti restire default setngs.

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• Show Scan Area outline: with this funotin the iuter edge area if tssue is marked with an iutline ti shiw the exaot area that has been soanned. This funotin is useful ti mark the soanned area if the s eoimen.

• Scale bar: the soale bar dis layed in the slide windiw oan be switohed in by olioking ir if by olioking if neoessary.

The oilir if the soale bar oan be midifed afer olioking . Nite, that the oilir ifthe soale bar oannit be set ti red, as it is used fir resentng high-resilutin if the slide. The oilir used aotually is resented in the oilir bix next ti this buttin. Ti defne the baokgriund oilir if the soale bar, aotvate the Vackground i tin, then seleot Semi-transparent ir Opaque.

• 3D Peek video folder: The filder fir stiring videis oreated with 3D Peek feature if the rigram oan be set here.

• - Hardware settings

• Mult-core support: the rigram is oa able ti use mult le CPU oires ti im rive the s eed if rioessing. The maximum number if oires that is su irted is 16.

• Available slide cache memory: Size if memiry allioatin fir oaohing slide data

• Preferred usage rate: Peroentage if available RAM used fir oaohing

• Remove case URL if unused: oase URL, if nit in use, will be oheoked and remived afer the s eoifed tme has assed.

• Optmiiaton for Slow Connectons: Enable when using sliw oinneotin

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• Peripheral devices

• Inverted mouse ioom: aotvate this i tin ti invert direotin if ziiming with the miuse wheel.

• Use virtual or touch keyboard: u in olioking ir tiuohing a textbix in the GUI, the Windiws On-Soreen Keybiard (OSK) a ears in the soreen.

• SlideDriver: enables ir disables SlideDriver devioe fir mire efoient use if the a lioatin. When using SlideDriver fir the frst tme, the hardware must be deteoted by olioking the auti deteot hardware setngs buttin.

Seleot Zoom dependent ir Fixed mitin i tin ti set mitin de endent frim the magnifoatin level used ir ti use a fxed value between ste s during miving the slide image.

The s eed if anning oan be set with the Speed slider.

Seleot Clockwise ir Ant-clockwise direotin if ritatin fir u /diwn and lef/right mivement.

The COM irt if the devioe is seleotable frim the dri -diwn list. If yiu di nit kniw the s eoifo irt, oliok Auto Detect.

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• - Annotaton Scales

If yiu want ti Hide Annotaton menu when changing slide, turn in this funotinality.

The size if annitatins oan be set in miorimeters ir millimeters.

• Fix rectangular: Ty e ir oliok arriws ti set values. Area is oaloulated and dis layed autimatoally.

• Fix circle: Ty e ir oliok the arriw buttins ti set diameter value if the annitatin.

• Fix 20 / Fix 40: Smaller adjustments oan be made by dragging the sliders fir bith 20mm and 40mm FOVs.

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• - Annotaton propertes

• Show Name

◦ Auto naming: Aotvate this funotin ti auti-name annitatins with the set refx text (default: Annitatin).

◦ Auto numbering: Autimated numbering if annitatins oan be aotvated here.

◦ Annotaton prefx: Ty e a tem late fir autimated annitatin naming and numbering.

◦ TMA prefx: Ty e a tem late fir autimated naming and numbering if TMA markers.

• Show Annotaton Siie: The size if annitatins are dis layed if switohed in.

• Font siie: Fint sizes if the filliwing attributes oan be set here:

◦ Slide Name

◦ Annotaton Label

• Outline color: The default iutline oilir if Brightield and Fluiresoent slide annitatins, and the seleotin oan be defned. Cliok the alette buttin if a redefned oilir ti midify it.

• Title color: The default oilir if the annitatin ttle and the baokgriund bith fir brightield and fuiresoent slides oan be defned. Cliok the alette buttin if a redefned oilir ti midify it.

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• - Advanced settings

• Multple Instances: Enable/Disable running mult le instanoes

• Applicaton Registraton

◦ Registered as Default Viewer: Set SlideViewer as default viewer fir slides

◦ Registered as Default Applicaton for .mrxs files: Set SlideViewer as default viewer fir .mrxs slides

• Service Mode: Enable/Disable Servioe Mide

Important!

This i tin is asswird riteoted, the filliwing setngs are available fir yiu ti set inly if yiu ty e in the asswird fir authentoatin.

◦ Sttching Filter: Enable/Disable Sttohing Filter

◦ Intensity Sttching: Enable/Disable Intensity Sttohing

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◦ FOV Rectangles: Enable/Disable dis laying if FOVs in the slide

◦ FOV Rectangles Focus Type: Enable/Disable dis laying if a Fious Ma shiwing oiurse and fne fious iints used during soanning

Cliok ti save midifoatins when fnished.

Note:

Servioe mide setngs (if aotve) will nit be autimatoally aooessible afer olising SlideViewer. If Servioe mide is turned in and aooessible, but the Setngs anel is olised, the buttin is dis layed.

16. Z-Stack panel

Panniramio soanners are oa able if aoquiring -Staok images (oinsistng if several layers if diferent fious levels). By default, the viewer dis lays the si-oalled Extended Focus Level (fir details see the user guide if the s eoifo soanner).

-Staok mide is aotvated and dis layed as by default, itherwise oliok ti turn in -Staok mide. Diferent -Staok levels oan be viewed by miving the slider u - ir diwnwards by dragging ir sorilling. The desired value oan be entered in the indioatir feld that shiws the aotual distanoe frim the oenter fioal level in miorimeters.

Ti turn in -Staok re-fetohing oliok , thus transitin between layers beoimes smiith as the maximum if 17 layers (8 abive and 8 beliw the inital layer) are re-liaded by the sifware.

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17. MultView toolbox

SlideViewer alliws arallel and synohrinized view if maximum nine slides at the same tme. S litter lines (birders if the anes) oan be mived, making it available fir yiu ti midify the ane area in whioh the slide oan have mire s aoe in the dis lay.

Cliok ti seleot the number if slides ti be dis layed in the MultView windiw, then drag the area if maximum 3x3 anes.

• – Slides oan be linked ti eaoh ither, si they oan be mived and magnifed in a synohrinized way.

• – Aligns the slides in the inaotve anes ti the slide in the aotve ine, henoe the same irientatin and ziim level are ada ted and any midifoatin (in ziim level and navigatin) be a lied fir all if the dis layed slide images.

• – O ens all slides startng with the seleoted slide in the oase ir filder.

• and – Liads the next/ reviius batoh slide within the oase ir filder de ending in the seleoted number if views.

In MultView mide frst yiu must oliok the ane if the slide ti seleot it befire managing ir making any midifoatins ti that slide.

• – Liads the next/ reviius slide within the oase ir Teleoinsultatin sessiin. This is a built-in funotin if SlideDriver as well.

If olioking the buttin the slides will be dis layed in slide staok dis lay mide. The transitin oan beoime smiith between slides when dragging the slider gently between the layers/ste s ir oliok at

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the slider nitohes ti view diferent slides. If using the Ctrl+F11 shirtout, the seleoted view in

MultView mide is dis layed in single-view. Cliok ti return ti MultView mide.

– Slide stack export: A Virtual tray is oreated ointaining the slides that are i en in MultView mide inti the filder if the frst slide. This funotin is available fir slides i ened frim SlideCenter server.

18. Quick slide access

If yiu want ti i en anither slide frim anither lioatin, frst oliok ti aooess Local ir SlideCenterfilder if the aotually seleoted slide, then briwse fir the slide ir drag the slide inti ine if the anes.

At the lef bittim ane the aths if the reviiusly i ened slides are listed. This list is hidden by default, and oan be i ened by olioking the buttin.

If yiu oliok in any if the listed aths, the ointent if the filder is dis layed as slide thumbnails at the right bittim ane.

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