SolCAPSolanaceae Coordinated Agricultural ProjectDedicated to the Improvement of Potato and Tomato

Executive Commitee :David Douches      Walter De Jong    Robin Buell     David Francis

Alexandra Stone    Lukas Mueller      AllenVan Deynze

Supported by the National Research Initiative Plant Genome Program of USDA’s Cooperative State Research , Education and Extension Service

What is SolCAP?

The SolCAP project links together people from public institutions, private institutions and industries who are dedicated to the improvement of the Solanaceae crops: potato and tomato.

Through innovative research, education and training the SolCAP project will focus on providing significant benefits to both the consumer and the environment.

The SolCAP project is supported by the Agriculture and Food Research Initiative (AFRI) Applied Plant Genomics Program of the USDA’s Cooperative State Research, Education and Extension Service

SolCAP Management Structure

Stakeholder Advisory BoardDr. Robert Hoopes, Frito‐LayErik Legg, SyngentaDr. Thomas Osborn, SeminisChuck Rivara, CTRIDr. Caius Rommens, Simplot

Scientific Advisory BoardDr. Glenn BryanDr. James GiovannoniDr. David NealeDr. Dani Zamir

Executive CommitteeDr. Robin BuellDr. Walter De JongDr. David FrancisDr. Alexandra StoneDr. Allen Van DeynzeDr. Lukas Mueller

Project AssistantKelly Zarka

GenotypingFrancis

Van Deynze

Data ManagementBuell

Mueller

Small Grants ProgramFor Genotyping Trait Specific Populations

Executive Committee

Germplasm PanelsDouchesFrancis

EducationDe Jong

Van Deynze

ExtensionStoneLiedlFrancis

Extension Advisory BoardDr. Deana Namuth CovertDr. Deborah Lewis

Education and Extension AssessmentDr. Michael Coe

SolCAP Advisory Board

Project DirectorDr. David Douches

MinnesotaUniversity of Minnesota WisconsinUSDA/ARS University of WisconsinMichiganMichigan State UniversityOhio Ohio State University

Lead Institution: Michigan State University

OregonOregon State UniversityCedar Lake Research and ConsultingIdahoUSDA/ARS University of IdahoCaliforniaUC DavisCampbells R&D

New YorkCornell UniversityMarylandUSDA/ARS Beltsville

West VirginiaWest Virginia State UniversityNorth CarolinaNorth Carolina State UniversityFloridaUniversity of Florida

SolCAP Project Participants

SolCAP Project OverviewResearch - Education - Extension [Potato Example]

‐mRNA ‐> cDNA ‐> Transcriptome‐Snowden, Atlantic, Premier Russet

‐Solexa sequencing of new ESTs‐50 bp reads with alignment‐new eSNPs

Sequencing‐eSNPs identified from previous ESTs

(Bintje, Shepody, Kennebec )‐7700 potato

‐200 Candidate genes‐SCRI 700 validated SNPs‐Dutch 400 validated SNPs‐New eSNPs from SolCAP sequencing‐Interface with SGN

‐eSNPs‐Panel phenotypic data‐Panel genotypic data

Bioinformatics

‐Infinium platform (7600 SNPs)‐1200 genotypes per run

‐Germplasm panel (500)‐Additional mapping populations (1900)

‐Phenotyping by breeder‐Genotyping by SolCAP‐Determined through Small Grants Program

Illumina Genotyping

‐Potato elite germplasm‐300 clones & genetic lines‐200 4x mapping (QTL)‐100 2x mapping (QTL)‐14 species‐genetic stocks

‐Descriptor database (literature)‐Phenotyping, 2 yrs, 3 locs

‐NY, WI, OR (250 clones)‐NC, ID, MN 4x mapping (200 progeny)‐Key traits: CHO, starch, vitamins

Germplasm Panels‐SNP mapping (2x)‐Haplotype analysis‐Association mapping for key traits

‐CHO, starch‐Germplasm relationships‐Design further mapping populations‐QTL identification for key traits (4x)

Genotype Panels Studies

‐Plant Breeding and Genomics Community of Practice (CoP)‐Target breeding community‐Coordinated activity of ALL CAPs to create content

‐Sol (lead)‐Rice‐Barley‐Wheat‐Conifer‐Onion‐other

‐Survey‐based Assessment‐Needs‐Impact measurement

eXtension.org

‐Graduate students‐Graduate class (distance learning)‐Translational Genomics

‐Breeding community‐Workshops (1/2 day)‐PAA‐other

‐Workshops for‐Bioinformatics‐SGN‐QTL‐Markers‐Mapping‐MAS/MAB

‐Survey‐based Assessment‐Needs‐Impact measurement

Multiple Targets

‐Database for germplasm panel

‐Genotype‐Phenotype‐Descriptors

‐Database for SNPs‐Breeders Toolbox

SGN

‐QTL validation‐New mapping populations

‐10 additional (1900 genotypes)‐New traits

‐Marker conversion of SNP to agarose‐based‐MAS/MAB

Small Grants Program

‐Improved breeding‐Methods‐Efficiency

‐New varieties

Breeders Apply MAS/MAB

Visit us athttp://solcap.msu.edu