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Carcinogenesis vol.35 no.8 pp.1691–1697, 2014doi:10.1093/carcin/bgu022Advance Access publication January 22, 2014

Speckle-type POZ protein, SPOP, is involved in the DNA damage response

Dan Zhang1,2, Haibo Wang2, Mianen Sun2,3, Jun Yang2, Wei Zhang4, Suxia Han1 and Bo Xu2,5,6,* 1Department of Oncology, The First Affiliated Hospital of Xi’an Jiaotong University Medical College, Xi’An, Shaanxi 710061, China, 2Department of Radiation Oncology, Houston Methodist Research Institute, Houston, TX 77030, USA, 3Department of Genitourinary Medical Oncology, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA, 4Department of Organic Chemistry and 5Department of Oncology, Southern Research Institute, Birmingham, AL 35205, USA and 6Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35205, USA

*To whom correspondence should be addressed. Tel: 205 581 2845; Fax: 205 581 2907; Email: xu@sri.org

Correspondence may also be addressed to Suxia Han. Tel: (+86) 29 8532 3472; Fax: (+86) 29 8532 3472; Email: shan87@mail.xjtu.edu.cn

Speckle-type POZ protein (SPOP) is an adaptor of the cullin 3-based ubiquitin ligase responsible for the degradation of oncoproteins fre-quently overexpressed in many tumor cells. Altered expression and somatic mutations of SPOP have been observed in various tumor types with chromosomal aberrations, indicating a role of SPOP in maintaining genome stability, although a detailed mechanism remains unclear. Here, we show that SPOP is a component of the DNA damage response (DDR). SPOP is recruited to DNA double-strand break sites and it forms nuclear foci after DNA damage. SPOP foci colocalize with γ-H2AX foci and are predominantly dependent on the activity of the ataxia-telangiectasia mutated (ATM) kinase. Furthermore, SPOP interacts with ATM in response to DNA dam-age. Finally, we demonstrate that knocking down of SPOP resulted in an impaired DDR and a hypersensitivity to ionizing irradiation. Together, we highlight a critical role of SPOP in the DDR.

Introduction

An optimal DNA damage response (DDR) is critical for mammalian cells to maintain genome stability (1). Orchestrated by a comprehensive sign-aling network, the DDR reacts to environmental DNA damaging stress (both from endogenous and exogenous sources) by initiation of cell cycle checkpoints, DNA repair, and programmed cell death mechanisms. The overall functions of the DDR seek to eliminate or reduce the risk of erro-neous DNA replication being passed to daughter cells. Suboptimal DDRs have been well documented in a majority of tumor cells, indicating a role for DDR proteins as tumor suppressors (2). It is also believed that acti-vation of optimal DDR plays a critical role as an antitumor barrier dur-ing early tumorigenesis (3–5). On the other hand, optimal DDR is also responsible for cell survival in response to DNA damaging agents, such ionizing irradiation (IR) and many of the chemotherapeutic drugs (6). Additionally, recent evidence also suggests that hyperactive DDR might promote tumor invasion and metastasis (7,8). Therefore, elucidating the regulatory pathways of the DDR is critical to the understanding of tumor initiation, progression, and therapeutic responses.

Of the >100 genes, and their products, directly involved in the DDR, there are damage sensors, which recognize DNA strand breaks and recruit downstream proteins to damage sites; signal transducers, which amplify signals by posttranslational modification, and effec-tors, which typically are negative regulators of the cell cycle control.

These proteins are well conserved through evolution, and the func-tional significance of the signaling transduction pathways has been extensively studied. For example, the ataxia-telangiectasia mutated (ATM) protein, mutation of which causes the autosomal recessive genetic disease ataxia telangiectasia, can be recruited and activated in response to DNA double-strand breaks (DSBs) (9). Activated ATM kinase then phosphorylates a large number of proteins to facilitate an optimal DDR (10). For example, ATM phosphorylation of Brca1 (11,12) inhibitor 2 (13), and deoxycytidine kinase (14) are critical for cells to initiate the G2/M cell cycle checkpoint.

E3 ubiquitin ligases play critical roles in cellular functions, includ-ing the DDR, by targeting specific protein substrates for proteasome-mediated degradation (15). Speckle-type poxvirus and zinc finger protein, SPOP, serves as an adaptor for cullin 3 (Cul3)-based ubiq-uitin ligases, a large family of the E3 ubiquitin ligases (16). SPOP is a nuclear protein belongs to the MATH-BTB protein family and was originally identified by immunoscreening as an autoantigen in a scleroderma patient sample (17). Substrates of SPOP include the apoptosis factor DAXX (16), the breast cancer metastasis suppres-sor BRMS1 (18) and the Hedgehog signaling transcription factors Gli2 and Gli3 (19–21). In addition, drosophila SPOP mediates deg-radation of the Jun kinase phosphatase puckered, resulting in tumor necrosis factor/Eiger-dependent apoptosis (22). Recently, SPOP was characterized as a tumor suppresser due to its role in reducing the cellular level of the p160 steroid receptor coactivator SRC-3 onco-protein (23,24). Additionally, SPOP was identified as a regulator in response to fludarabine treatment in chronic lymphocytic leukemia cells (25). Recent literature has shown that loss of SPOP expression was common in gastric cancer, colorectal cancer as well as prostate cancer (26). More interestingly, exome sequencing studies have iden-tified SPOP as the gene most commonly affected by somatic non-synonymous point mutations in prostate cancer (27–30). Reduced expression, as well as somatic mutations, of SPOP is associated with chromosomal aberrations in the cancers, indicating a role of SPOP in the maintenance of genome stability.

Here, we report that SPOP is an essential element of the DDR reg-ulatory network. In response to DNA damage, SPOP interacts with ATM and forms nuclear foci. Depletion of SPOP in cells results in an impaired DDR and an increased radiosensitivity.

Materials and methods

Cell cultureThe human cervical cancer cell line HeLa, the human breast cancer cell line MDA-MB-231 and the human osteosarcoma cell line U2OS, all obtained from The American Type Culture Collection (Manassas, VA), were cultured in Dulbecco’s modified Eagle’s medium (Hyclone, Logan, UT) with 10% fetal bovine serum (Hyclone). All cell lines were grown in a 5% CO2 incubator at 37°C. Ionizing radiation was delivered by an X-Rad 320 X-ray irradiator (Precision X-Ray, North Branford, CT).

Antibodies and siRNA oligosThe SPOP antibody was purchased from Santa Cruz Biotechnology (Dallas, TX), and the dilution used was 1:1000. The γ-H2AX antibody was purchased from Cell Signaling Technology (Danvers, MA), and the dilution used was 1:5000. The ATM antibody and phosphor-Serine 1981 ATM antibody were purchased from Abcam (Cambridge, MA), and the dilutions used were 1:3000 for both. The control small interfering RNA (siRNA) and two independent SPOP siRNA oligos were purchased from Santa Cruz Biotechnology and Dharmcon (Waltham, MA), and 20 nM oligos were used in each experiment. The ATM siRNA oligo (5′-CACCUUUUUCUUGGGUUUUGGCUCCUU-3′) was developed by our lab, and 20 nM oligo was used in this study. siRNAs were transfected into cells by oligofectamine reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instruction.

Western blottingCells were incubated with the lysis buffer (Fisher, Pittsburgh, PA) containing the protease inhibitor cocktail (Roche, Indianapolis, IN), and the protein concentration

Abbreviations: ATM, ataxia-telangiectasia mutated; Cul3, cullin 3; DDR, DNA damage response; DSB, double-strand break; IR, ionizing irradiation; mRNA, messenger RNA; PBS, phosphate-buffered saline; SBC, SPOP-binding consensus; siRNA, small interfering RNA; SPOP, Speckle-type POZ protein.

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was determined using the DC Protein Assay kit (Bio-Rad, Hercules, CA). The 4–12% Bis–Tris precast gels (Bio-Rad) were used for electrophoresis. Equal volumes of cell total protein were loaded and subsequently transferred from gels to the nitrocellulose membrane. The membrane was blotted with 5% of non-fat milk (Lab Scientific, Livingston, NJ), followed by the incubation with primary and horseradish peroxidase-conjugated secondary antibodies for overnight or 2 h, respectively. Signals were detected by adding chemilumines-cence reagents (GE, Buckinghamshire, UK).

RNA isolation and real-time PCRTotal RNA was isolated from cells using RNAfast200 (Pioneer Biotechnology, Shaanxi, China). Five hundred nanograms of RNA was used to synthesize complementary DNA by using PrimeScript™ RT Master Mix (Takara Biotechnology, Dalian, China). The SPOP primer/probe sets (SPOP-F: 5′-ATTCCAGGCTCACAAGGCTATCTTA-3′, SPOP-R: 5′-CAGCTGCCAG CAAATCATCA-3′) and the SYBR® Premix Ex Taq™ II for real-time PCR were purchased from Takara Biotechnology. All real-time PCR experiments were performed in triplicate.

Cell proliferation assay and clonogenic survival assayThe cell proliferation assay was performed on the RNA oligos-transfected HeLa cells seeded in 96-well plates. Following the treatment with mock or irradiation, cell proliferation was detected by Cell Titer 96@ AQueous One Solution Cell Proliferation Assay system (Promega, Madison, WI) at 0, 24 and 48 h after the treatment. Generally, 20 μl of the One Solution reagent was loaded to each well and the mixture of the reagent and cells were put into incu-bator for 2 h. Cell proliferation was then measured using a microplate reader (BioRad Model 680) with the absorbance of 490 nm. The clonogenic survival assay was performed on the RNA oligos-transfected HeLa cells growing in 6-well plates. The plates were subjected to irradiation with indicated doses and, 10–14 days later, cells were stained with crystal violet (0.1%) for 30 min. The colonies with 50 or more cells were then scored. The survival fractions were calculated as described previously (31).

Immunofluorescence and immunoprecipitationCells plated on glass slides (Fisher) were fixed with 4% paraformaldehyde for 15 min and permeabilized in 0.5% Triton X-100 for 10 min. Cells were rinsed with phosphate-buffered saline (PBS) after the indicated treatment and incubated with primary antibody diluted in 1% bovine serum albumin 1 h at room tempera-ture or overnight at 4°C. Cells were washed three times with 0.1% PBS-T and incubated with fluorescent-conjugated secondary antibody diluted in 1% bovine serum albumin for 1 h at room temperature in the dark. Cells were washed three times with 0.1% PBS-T. 4′,6-diamidino-2-phenylindole (1 mg/ml) diluted in 1× PBS was used to stain DNA for nuclear visibility. Slides were mounted using microscope immersion oil and visualized using a fluorescence microscope. For immunoprecipitation, cells were lysed with lysis buffer following harvest. The cell lysates were incubated with Protein G Sepharose (Sigma–Aldrich, St Louis,

MO) and the SPOP antibody, the ATM antibody, goat IgG or rabbit IgG, respec-tively, overnight at 4°C. The immunoprecipitates were washed three times with the IP Washing Buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol), then subjected to western blotting.

Single cell gel electrophoresis assay (comet assay)Comet Assay kits, purchased from Trevigen (Gaithersburg, MD), were used in this study. The neutral comet assay was performed according to the manufactur-er’s instructions. Generally, cells were transfected with siRNA oligos and irradi-ated with mock or IR. Cells were then harvested 0, 4 and 8 h after IR and mixed with low-melting agarose for the subsequent electrophoresis. The nuclear DNA was stained with SYBR green dye for 10 min and visualized using a fluorescent microscope. Olive tail moment was measured using CASP software.

StatisticsData were determined by Student’s t-test and P values ≤0.05 were considered significant.

Results

SPOP forms nuclear foci in response to DNA damageTo characterize SPOP’s functions in the DDR, we first examined if SPOP is recruited to DNA damage-induced DSBs. In unper-turbed HeLa cells, SPOP expression is diffused with weak speck-les. However, when cells were treated with IR (4 Gy), we observed nuclear focus formation of SPOP (Figure 1) 1 h after IR. The SPOP foci apparently colocalized with the foci of γ-H2AX, a phosphoryl-ated form of the histone variant H2AX and a marker of DNA DSBs (32). Therefore, these observations indicate that SPOP is recruited to DSBs in response to DNA damage. Similar patterns of focus for-mation and colocalization were observed in HeLa cells treated with camptothecin, a DNA topoisomerase I  inhibitor. This phenotype is also observed in other cell lines including the breast cancer cell line MDA-MB-231 and the osteosarcoma cell line U2OS (Supplementary Figure S1, available at Carcinogenesis Online).

To assess if SPOP messenger RNA (mRNA) levels changes after IR, we conducted reverse transcription–polymerase chain reaction to assess SPOP mRNA changes 1, 6, 12, 24, 48 h after IR (4 Gy) in HeLa cells. We have also assessed the changes of the protein level. As shown in Supplementary Figure S2, available at Carcinogenesis Online, SPOP mRNA did not show significant changes in the 1 and 6 h time points, but it displayed temporary increases 12–24 h after IR. The increase of mRNA was correlated with the protein level.

SPOP γ-H2AX Merge DAPI

Mock

IR

CPT

Fig. 1. SPOP forms nuclear foci in response to DNA damage. Exponentially growing HeLa cells were treated with mock, IR (4 Gy) or camptothecin (CPT, 10 μM). One hour after IR and 2 h after CPT, cells were fixed and immunostained with the anti-SPOP (red) or anti-γ-H2AX (green) antibody. 4′,6-diamidino-2-phenylindole (DAPI) was used for DNA staining (blue).

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ATM activity is required for SPOP nuclear focus formation in the DDROwing to the central role of the ATM kinase in the DDR, we tested whether DNA damage-induced SPOP focus formation is dependent on ATM. To achieve this goal, we knocked down endogenous ATM

by transient transfection of ATM siRNA into HeLa cells. We found that ATM depletion significantly reduced the formation of SPOP foci (Figure 2A and B). Inhibition of ATM by a specific ATM inhibitor Ku55933 also achieved a similar effect. It is noted that ATM depletion or inhibition did not alter the whole protein level of SPOP (Figure 2C).

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Fig. 2. ATM is involved in the formation of SPOP nuclear foci in response to DNA damage. HeLa cells transfected with control or ATM siRNA or treated with Ku55933 (10 nM) were subjected to immunofluorescence microscopy (A) or immunoblotting (C) using indicated antibodies. (A) The localization of SPOP (red) and γ-H2AX (green) 1 h after IR (4 Gy). (B) Quantitative data of foci/nuclear of SPOP. (C and D) Endogenous expression levels of SPOP in HeLa cells transfected with control or ATM siRNA (C) or treated with Ku55933 (10 µM, 2 h) (D) in the presence or absence of IR (4 Gy, 1 h).

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SPOP interacts with ATM in response to IRWe further investigated if there is an interaction between SPOP and ATM by coimmunoprecipitation. We did not observe an interaction of SPOP and ATM in mock-treated cells; however, we observed that ATM was present in the SPOP immunoprecipitates in response to IR (Figure 3A). A reciprocal experiment showed that SPOP was detect-able in the ATM immunoprecipitates only after cells were irradiated (Figure 3B). These results indicate that SPOP interacts with ATM in response to IR-induced DNA damage.

SPOP knockdown resulted in suboptimal DNA damage repairRecruitment of SPOP to DNA DSB sites shown in Figure 1 indicates that SPOP might be required for DNA damage repair. Therefore, we examined if depletion of endogenous SPOP by siRNA impacted DNA damage repair processes. Two independent siRNA oligos were used to knockdown SPOP in HeLa cells, and both oligos were efficient in reducing the SPOP level (Figure 4A). We first investigated the exist-ence of γ-H2AX foci in control or SPOP-depleted cells in response to IR. In control cells, the number of γ-H2AX foci per nucleus increased at 1 h after treatment and returned to a slightly above normal level at 24 h posttreatment because of the repair of the DSBs. However, SPOP knockdown cells showed a prolonged presence of γ-H2AX foci after IR (Figure 4B). This strongly suggests that SPOP is required for efficient DSB repair. We also conducted a neutral gel comet assay and the results are consistent with the γ-H2AX foci (Figure 4C), as sig-nificantly higher olive tail moment in SPOP knockdown cells were observed, indicating a delay of DNA strand break repair in the absence of SPOP.

SPOP depletion results in a hypersensitivity to IRWe further investigated the effect of SPOP knockdown on cellu-lar radiosensitivity. Using a cell proliferation assay, we found that, although SPOP knockdown in the absence of DNA damage caused mild cell death, it greatly sensitized HeLa cells to IR (Figure  5A). We also conducted the clonogenic survival assay assessing radio-sensitivity. Compared with control cells, SPOP knockdown cells showed a significant reduction in cell survival after IR (Figure 5B). Together, our data demonstrate a critical role of SPOP in regulation of radiosensitivity.

Discussion

Characterized as an adaptor protein in the Cul3 E3 ubiquitin ligase family, SPOP has recently been shown as a tumor suppressor for the proteolysis of the oncogenic coactivator SRC-3/AIB1 (23,24). Somatic mutations as well as altered expressions of SPOP have been observed in many tumor types, including gastric cancer, colorectal cancer as well as prostate cancer (26,29), indicating a potential role of SPOP in genome stability. In this manuscript, we reported, for the first time, that SPOP is an essential element of the DDR. SPOP forms nuclear foci and interacts with ATM in response to DNA damage. Functionally, SPOP is required for DNA damage repair and the main-tenance of radioresistance. These observations provide insights into the functional significance of SPOP in genome stability.

As a speckle-type protein, a rough speckled pattern of endog-enous SPOP was originally shown in the nuclei of COS7 cell line (17). However, there were very few speckles detected for the endog-enous SPOP in HeLa cells (33). Consistent with the previous data, few bright spots of endogenous SPOP were visualized. However, in response to DNA damage, a significant number of nuclear foci were formed. Colocalization of SPOP foci with γ-H2AX foci supports DSB-dependent nuclear recruitment of SPOP in the event of DNA damage. Although at later time points after IR, SPOP mRNA and pro-tein levels increases temporarily, the SPOP focus formation appears to be mediated by posttranslational modification.

Ubiquitination plays a critical role in the DDR as evidenced by that fact that many E3 ubiquitin ligases such as RNF8 and RNF168 catalyze polyubiquitylation of histones H2A and H2AX at DSB sites (34,35). Recent reports have shown that ATM-dependent phospho-rylation of the E3 ubiquitin ligase, RNF20-RNF40 heterodimer, is responsible for histone H2B monoubiquitylation, which facilitates the two major DSB repair pathways: non-homologous end-joining and homologous recombination repair (36). A  link between SPOP and ATM might provide clues on how SPOP is involved in the DDR. The requirement of ATM activity in SPOP focus formation indicates that ATM is an upstream of the SPOP pathway. Indeed, SPOP contains three ATM consensus phosphorylation motifs (S/T-Q, threonine 25, serine 119 and threonine 319), and one of them, serine 119, located in the conserved N-terminus MATH domain that recruits substrates (37), is found to be mutated (a substitution of serine to asparagine -S119N)

IB: ATM

IB: SPOP

IB: SPOP

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Mock IR IgG SPOP IgG SPOP

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Fig. 3. SPOP interacts with ATM in response to DNA damage. Endogenous SPOP (in A) or ATM (in B) was immunoprecipitated in cells 1 h after treating with mock or IR (4 Gy). The immunoprecipitates were subjected to immunoblotting using indicated antibodies. Total cell lysates were included as loading controls.

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in some prostate cancers (29). In a recent study, it has been shown that induction of S119N mutation in LNCAP-ABL cells abrogated degradation of SRC3 (24), indicating an important role of the residue and the surrounding sequence.

The solved crystal structure of SPOP (PDB 3HQI) demonstrates the heterodimeric assembly of the SPOP–Cul3 interaction. The sec-ondary structure of SPOP contains a conserved α-helical C-terminus which encompasses the BTB domains, also known as the ‘3-box’ due to its facilitating of Cul3 binding, as well as its similarity to F-/SOCS-boxes in other cullin-based E3s (37). The structural flex-ibility attributed to the region between the MATH and Cul3-binding BTB/3-box domains most likely allows for a SPOP dimer to simulta-neously interact with multiple SPOP-binding consensus (SBCs) on a

substrate. This allows for high-order hetero-oligmerization that facil-itates the ubiquitination of bound substrates (38). The most recent crystal structure of the dimeric SPOP C-terminal domain showed that tyrosine 353 plays a critical role in high-order oligomerization (39). To assess a potential role of ATM-mediated SPOP phospho-rylation, we have conducted molecular modeling studies to obtain the structural insight into the ATM consensus motifs on SPOP in order to predict the potential effects of ATM-mediated phosphoryla-tion (Figure  6). The structural models of SPOPMATH and SPOPBTB were built directly from the SPOP crystal structure (PDB ID: 3HQI) using the protein preparation protocol of Schrödinger®. We found that, unlike threonine 25, which belongs to the flexible N-terminus of SPOP, both serine 119 and threonine 319 locate at structurally

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Fig. 4. SPOP knockdown leads to defects in DNA repair. Two independent siRNA oligos were used to inhibit endogenous expression of SPOP in HeLa cells. Twenty-four hours after transfection of control or SPOP siRNA, cells were subjected to IR (4 Gy). One hour after IR, cells were harvested and subjected to the following assays: (A) Immunoblotting of endogenous SPOP after siRNA knockdown. (B) Fluorescence microscopy staining and quantification of the existence of γ-H2AX foci. (C) The signal cell electrophoresis assay (the comet assay) and quantification of olive tail moment.

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conserved regions that are important for SPOP functions. The crystal structure of the SBC-SPOPMATH complex indicates that serine 119 locates at the SBC-MATH binding interface and is in close contact with the non-polar residue of the SBC motif (37). Phosphorylation of serine 119 therefore will directly affect SPOP-substrate binding. On the other hand, threonine 319 is a part of the Cul3-interacting box region at the C-end of the SPOP BTB domain (SPOPBTB) (37). Similar as the F-/SOCS-boxes that bind to cullins, the ‘3-box’ is functionally important for SPOP-Cul3 binding. Although structural information regarding the SPOP–Cul3 interaction is still not avail-able, the crystal structure of the SPOPBTB has demonstrated that the ‘3-box’ is a relative small fragment consisting two short helixes; threonine 319 locates in the middle of the helical region and is fully solvent exposed (which is typical for residues at protein–protein interfaces). Thus, it is very likely that phosphorylation of threonine 319 will affect the interactions between SPOP and Cul3.

Despite the evidence that SPOP depletion cause pronged H2AX foci and higher comet tail moment, it is still not known whether SPOP directly participates in the DNA repair processes. Due to its known role as an adaptor protein of Cul-3-mediated ubiquitination, it is likely that SPOP participate in the DDR as an adaptor for multiple signaling in response to DNA damage, thus playing an indirect role in DNA repair.

In summary, we demonstrate that SPOP is recruited to IR-induced DNA double-strand break sites in a predominantly ATM-dependent manner. We also show that SPOP depletion results in defects in the DDR and an increase in radiosensitivity.

Supplementary material

Supplementary Figures S1 and S2 can be found at http://carcin.oxfordjournals.org/

Funding

D.Z. was supported in part by funding from the China Scholarship Council; NIH grants (R01CA133093, R01ES016354 to Dr B.X.).

Acknowledgements

We thank Dr S.Mitra for helpful comments on the manuscript. We thank Dr R.Boohaker for the proofreading of the manuscript.

Conflict of Interest Statement: None declared.

Fig. 5. SPOP inhibition sensitizes HeLa cells to IR. HeLa cells were transfected with control or SPOP siRNA oligos (siSPOP-1 or siSPOP-2). Twenty-four hours after transfection, cells were irradiated with mock (0 Gy) or IR (4 Gy). At indicated time points after IR, cells were subjected to: (A) the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide proliferation assay (*indicates statistical significance, P < 0.05), and (B) the colony formation assay.

Fig. 6. Structural representation of the phosphorylation sites of serine 119 and threonine 319. (A) The SBC-SPOPMATH interface. The SBC residues are illustrated in green-colored sticks; the residues of SPOP are shown in gray lines; the serine 119 residue and the non-polar residue (V98) of SBC motif are highlighted in the space-filling model. (B) Carton representation of the SPOP-BTB domain. The threonine 319 residue is shown in the space-filling model. Both (A) and (B) are based on the crystal structure of SPOP (PDB ID: 3HQI).

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Received July 24, 2013; revised November 27, 2013; accepted December 20, 2013

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