1. Spectroscopy

2. AnalyticalChemistrySpectroscopyAnalysisSeparationAnalysisClassicalMethodInstrumentalMethodQualitativeAnalysisQuantitativeAnalysisChemical TestFlame TestGravimetricAnalysisVolumetric TitrationInfraredMassAtomic AbsorptionAtomic EmissionUV-VisibleNuclear MagneticResonancePaperChromatographyHPLCTLCLiquid /GasChromCatoolugmranp hyChromatography 3. Spectroscopythe study of interaction between electromagnetic radiationand matters.Particles (molecules, ions,atoms) can interact/adsorb aquantum of light.The instrument that perform the measurement is calledspectrometerMeasure the intensity of energy absorbed or emittedThe plot/graph recorded as a function ofwavelength/frequency is called Spectrum 4. SpectroscopySpectra are recorded :1. to observe how substance behave at certainenergy levels2. serves as identification tools for chemicalsubstance3. help predict the behaviour of unknown substanceor newly synthesized materials from ones alreadycharacterized 5. Spectroscopy3 types of spectroscopy:Absorption spectroscopy uses electromagneticspectra in which a substance absorbs: IR, NMREmission spectroscopy uses electromagneticspectra in which a substance emits: LuminescenceScattering spectroscopy measures the amount oflight that a substance scatter at certainwavelengths, incident angles and polarizationangles: Raman 6. Electromagnetic Spectrum 7. Revision 8. SpectrumContinuumSpectrumEmission LineSpectrumAbsorption LineSpectrumLight spectrum with all wavelength/frequencySpectrum with discrete wavelength/frequency(coloured lines)Emitted when excited electrons drop fromhigher to lower energy levelSpectrum with discrete wavelength/frequency(black lines)Absorbed when ground state electronsare excited 9. Emmission Spectroscopy vs AdsorptionSpectroscopyExcited stateGround stateAbsorption linespectrumElectron atexcited stateRadiationabsorbedAbsorb radiationto excited stateElectron fromground stateElectron fromexcited stateemit radiation whendrop to ground stateRadiationEmittedEmission linespectrum 10. Electromagnetic radiationRadiowaves Infra red UV-VisibleNuclear MagneticResonanceSpectroscopyInfrared SpectroscopyUV-VisSpectroscopyAtomicAbsorptionSpectroscopyOrganicstructuredeterminationMRI and bodyscanningOrganic structuredeterminationFunctional grp determinationMeasuring bond strengthMeasuring degree ofunsaturationin fatMeasuring level of alcohol inbreathQuantification of metal ionsDetection of metal invarious sample