sr christina joseph research powerpoint
TRANSCRIPT
The effect of the DSK gastro-peptide on feeding behaviors in Drosophila Melanogaster
By: Christina Mary JosephMentor: Dr. Monica Dus, Ph.DSkirball Institute of BioMedicine, NYU
Laying down the genetics
Laying Down the genetics
Known Behaviors
Sated starved
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2.11234766666667
Condition
Am
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Food ingestion assay of wildtype flies demonstrate the effect of satiety on ingestion rate
Known Behaviors
Sated starved
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87.8853728991595
W1118cs food availibilty assay
Condition of flyP
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f fl
ies
th
at
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Food availability assay of wildtype flies demonstrate the effect of satiety on commencement of eating under low availability circumstances
Experiments: PER Assay
Proboscis Extension Reflex Assay. Aids in determining feeding behaviors
Experiments: Ingestion Assay
The ingestion assay aids in determining quantity of food intake.
Experiments: Food availability assay
The food availability assay aids in determining ingestion initiation behaviors
What we found
Figure 1: Percentage of flies that extended their proboscis in both genotypes.
Figure 2: Trends of proboscis extension(TRPA1/DSK- Activated DSK neurons)
What we found
The amount of food eaten by the flies
(1.DSK/KG80- down regulation of DSK 2.DSK/NaChBac- up regulation of DSK)
DSK/+ DSK> kir2.1, tubulingal80ts
DsK>NAchBac0
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Ingestion assay
Genotypes
am
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What we found
Food availibility results(1.DSK/KG80- down regulation of DSK2.DSK/NaChBac- up regulation of DSK)
DSK/+ DSK> kir2.1, tubulingal80ts 102039 CCKLR-17D30
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50Food availibility assay: starved
Genotypes
Pe
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lies
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Conclusion
• The food availability assay showed that starved flies with increased levels of DSK tend to choose not to eat but it did not show starved flies with decreased levels of DSK ate more
• The food ingestion data showed amount eaten by the flies with less DSK ate greater portions than the control while flies with more DSK ate less portions than that of the control
• The PER assay showed that silencing of the DSK neurons led to increased proboscis extension
Discussion
• The data shows strong preliminary promise and a second round of experiments replicating the data shown here will allow us to move into a mammalian model; mice.
• If the data is replicated in the mammalian model, than eating disorders can be prevented by genetic manipulation also thereby preventing long term health defects such as heart disease, kidney failure and even cancer.
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