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Table S1

Biological Process GO-term#Seqs

GO:0008152 metabolic process 2013

GO:0009987 cellular process 1844

GO:0044699 single-organism process 1562

GO:0051179 localization 712

GO:0065007 biological regulation 502

GO:0050896 response to stimulus 215

GO:0071840 cellular component organization or biogenesis 142

GO:0023052 signaling 93

GO:0022610 biological adhesion 38

GO:0040011 locomotion 29

GO:0051704 multi-organism process 22

GO:0032502 developmental process 12

GO:0000003 reproduction 2

GO:0007610 behavior 1

Molecular Function GO-term#Seqs

GO:0003824 catalytic activity 1836

GO:0005488 binding 1383

GO:0005215 transporter activity 423

GO:0001071 nucleic acid binding transcription factor activity 251

GO:0060089 molecular transducer activity 88

GO:0005198 structural molecule activity 58

GO:0009055 electron carrier activity 51

GO:0016209 antioxidant activity 18

GO:0000988 transcription factor activity, protein binding 14

GO:0098772 molecular function regulator 9

GO:0045182 translation regulator activity 1

GO:0045735 nutrient reservoir activity 1

Cellular Component GO-term#Seqs

GO:0016020 membrane 852

GO:0005623 cell 549

GO:0032991 macromolecular complex 146

GO:0043226 organelle 79

GO:0005576 extracellular region 8

GO:0019012 virion 4

GO:0031012 extracellular matrix 3

GO:0009295 nucleoid 2

Table S2

Island start Island end Length Locus Gene start Gene end Strand Unique PWN146A Product

278152 304193 26041

PWN146_00256 278152 279255 1 Glutamate 5-kinase

PWN146_00257 279265 280518 1 Gamma-glutamyl phosphate reductase

PWN146_00259 280946 282118 1 Putative prophage CPS-53 integrase

PWN146_00260 282121 283380 1 hypothetical protein

PWN146_00261 283377 285167 1 AAA-like domain protein

PWN146_00262 285619 285873 -1 DNA-binding transcriptional regulator

PWN146_00263 285870 286595 -1 hypothetical protein

PWN146_00264 287158 287421 1 Prophage CP4-57 regulatory protein (AlpA)





PWN146_00269 288823 291156 1 DNA primase TraC

PWN146_00270 291641 292549 -1 Lipid A biosynthesis lauroyl acyltransferase

PWN146_00271 292908 293141 1 * hypothetical protein

PWN146_00272 293161 301743 -1 N,N'-diacetylchitobiose phosphorylase



457237 471447 14210

PWN146_00448 457237 457551 1 transcriptional regulator BolA

PWN146_00449 457919 459223 1 Trigger factor

PWN146_00450 459662 460285 1 ATP-dependent Clp protease proteolytic subunit precursor

PWN146_00451 460428 461699 1 ATP-dependent Clp protease ATP-binding subunit ClpX

PWN146_00452 461846 464248 1 Lon protease

PWN146_00453 464465 464737 1 DNA-binding protein HU-beta

PWN146_00454 464916 466799 1 Peptidyl-prolyl cis-trans isomerase D

PWN146_00455 466938 467321 1 ComE operon protein 1

PWN146_00456 467476 467889 1 Long-chain acyl-CoA thioesterase FadM

PWN146_00457 467925 468623 -1 7-cyano-7-deazaguanine synthase

PWN146_00458 468739 470457 -1 HTH-type transcriptional regulator SgrR

PWN146_00459 470562 471386 1 HMP-PP phosphatase

561698 577473 15775


PWN146_00550 561788 562555 -1 * Serine acetyltransferase

PWN146_00551 562616 563836 -1 * putative cysteine desulfurase

PWN146_00552 563833 565116 -1 * Phenylacetate-coenzyme A ligase

PWN146_00553 565113 566120 -1 * peptide synthase

PWN146_00554 566117 569227 -1 Tyrocidine synthase 3

PWN146_00555 569235 569810 -1 * hypothetical protein

PWN146_00556 569832 572117 -1 Linear gramicidin synthase subunit B

PWN146_00557 572155 573021 -1 Plipastatin synthase subunit D

PWN146_00558 574875 575531 -1 * Linear gramicidin synthase subunit D

PWN146_00559 576581 576691 1 * Cysteine--tRNA ligase

582140 605870 23730


PWN146_00568 582227 582859 -1 * Serine acetyltransferase

PWN146_00569 584304 585143 -1 * Phenylacetate-coenzyme A ligase

PWN146_00570 585787 586572 -1 * peptide synthase

PWN146_00571 586569 589679 -1 Tyrocidine synthase 3


PWN146_00573 590284 595305 -1 Linear gramicidin synthase subunit B

PWN146_00574 595319 602908 -1 Phthiocerol synthesis polyketide synthase type I PpsE

PWN146_00575 602901 603683 -1 Surfactin synthase thioesterase subunit

PWN146_00576 603765 604988 -1 * Inner membrane transport protein YdhC

2233867 2247705 13838

PWN146_02131 2233551 2234054 -1 * P22 tail accessory factor


PWN146_02133 2234368 2235657 -1 * P22 coat protein - gene protein 5

PWN146_02134 2235657 2236568 -1 * Bacteriophage, scaffolding protein


PWN146_02136 2238792 2239292 1 * AP2 domain protein

PWN146_02137 2239289 2240800 -1 * Terminase-like family protein

PWN146_02138 2240781 2241341 -1 * Terminase small subunit






PWN146_02144 2242934 2243383 -1 Bacteriophage lysis protein

PWN146_02145 2243380 2243991 -1 Chitinase class I

PWN146_02146 2243997 2244341 -1 Phage holin family (Lysis protein S)


PWN146_02148 2245190 2245573 -1 * Phage antitermination protein Q


PWN146_02150 2246205 2248397 -1 * Virulence-associated protein E

2254835 2264049 9214

PWN146_02162 2254791 2255660 1 * SPFH domain / Band 7 family protein

PWN146_02163 2255744 2257852 1 * DNA polymerase I

PWN146_02164 2258198 2259844 1 C-5 cytosine-specific DNA methylase

PWN146_02165 2259841 2260110 1 * VRR-NUC domain protein

PWN146_02166 2260245 2261078 1 * DNA adenine methylase



PWN146_02169 2262670 2263851 -1 Prophage CP4-57 integrase

PWN146_02513 2632993 2640123 -1 Gramicidin S synthase 2

PWN146_02514 2640145 2641056 -1 Methyltransferase domain protein

PWN146_02515 2641071 2642027 -1 P-aminobenzoate N-oxygenase AurF

3197092 3206242 9150


PWN146_03047 3197325 3197861 -1 Phage tail protein (Tail_P2_I)

PWN146_03048 3197848 3198765 -1 Baseplate J-like protein

PWN146_03049 3198740 3199099 -1 Gene 25-like lysozyme

PWN146_03050 3199137 3199754 -1 Phage-related baseplate assembly protein



PWN146_03053 3200968 3201303 -1 * Phage Head-Tail Attachment


PWN146_03055 3201747 3203834 -1 * ATP-dependent Clp protease proteolytic subunit

PWN146_03056 3203788 3205299 -1 Phage portal protein, lambda family


PWN146_03058 3205520 3207637 -1 Phage terminase large subunit (GpA)

3223301 3227380 4079


PWN146_03084 3223301 3223864 1 * DNA polymerase III subunit beta





PWN146_03089 3224834 3225076 1 Excisionase-like protein


PWN146_03091 3226412 3227380 1 Cysteine synthase A

3389291 3411464 22173

PWN146_03251 3388243 3389559 1 Terminase-like family protein


PWN146_03253 3391033 3391860 1 Phage Mu protein F like protein

PWN146_03254 3391857 3392309 1 Phage virion morphogenesis family protein





PWN146_03259 3395521 3396063 1 * Gp37 protein


PWN146_03261 3396283 3397716 1 * Phage tail sheath protein

PWN146_03262 3397716 3398240 1 * Phage tail tube protein FII



PWN146_03265 3399091 3401793 1 Phage-related minor tail protein

PWN146_03266 3401793 3402686 1 * Phage P2 GpU

PWN146_03267 3402686 3402910 1 * Phage Tail Protein X

PWN146_03268 3402898 3404130 1 * Phage late control gene D protein (GPD)

PWN146_03269 3404127 3404771 1 Phage-related baseplate assembly protein

PWN146_03270 3404862 3405221 1 * Gene 25-like lysozyme

PWN146_03271 3405212 3406321 1 Baseplate J-like protein

PWN146_03272 3406314 3406901 1 * Phage tail protein (Tail_P2_I)

PWN146_03273 3406905 3409598 1 Phage T4 tail fiber

PWN146_03274 3409598 3410128 1 Caudovirales tail fiber assembly protein

PWN146_03275 3410103 3411236 -1 Acyltransferase family protein

PWN146_03276 3411418 3412662 -1 Inner membrane transport protein YnfM

3530263 3538809 8546

PWN146_03387 3531081 3531749 1 * ATP-dependent Clp protease proteolytic subunit

PWN146_03388 3531954 3532577 -1 * transcriptional regulator FimZ


PWN146_03390 3533190 3533945 -1 Iron(3+)-hydroxamate import ATP-binding protein FhuC

PWN146_03391 3533938 3534930 -1 * iron-hydroxamate transporter permease subunit

PWN146_03392 3534920 3535879 -1 * Iron-uptake system permease protein FeuB

PWN146_03393 3535876 3536847 -1 * putative ABC transporter solute-binding protein YclQ precursor


PWN146_03395 3537209 3538291 -1 Bicyclomycin resistance protein

PWN146_03396 3538728 3539894 1 * transcriptional regulator BetI

4577717 4582485 4768PWN146_04334 4578298 4578549 -1 * DNA-binding transcriptional regulator

PWN146_04335 4578592 4579713 -1 Phage late control gene D protein (GPD)

PWN146_04336 4579872 4581062 1 Phage tail sheath protein

PWN146_04337 4581075 4581587 1 Phage tail tube protein FII

PWN146_04338 4581654 4581974 1 * Phage tail protein E

PWN146_04339 4582102 4585317 1 Chromosome partition protein Smc

4652198 4663867 11669

PWN146_04418 4652372 4653595 1 Prophage CP4-57 integrase










4822320 4836712 14392








PWN146_04579 4827891 4828052 -1 DNA-binding transcriptional regulator GadX

PWN146_04580 4828428 4829549 -1 * Transposase

PWN146_04581 4830773 4831201 -1 IS2 transposase TnpB

PWN146_04582 4831312 4832835 -1 * Group II intron-encoded protein LtrA

PWN146_04583 4834614 4835351 1 * Integrase core domain protein

PWN146_04584 4835348 4836139 1 DNA replication protein DnaC

5201721 5226243 24522

PWN146_04920 5201721 5202431 1 Transcriptional repressor PifC

PWN146_04921 5202535 5202894 1 transport protein TonB

PWN146_04922 5202962 5203300 1 Colicin E1 (microcin) immunity protein

PWN146_04923 5203317 5205059 -1 Colicin-Ia


PWN146_04925 5205901 5208138 -1 Colicin-D






PWN146_04931 5212519 5213286 -1 Integrase core domain protein

PWN146_04932 5213337 5213603 -1 Transposase

PWN146_04933 5214378 5215190 -1 * Putative sporulation-specific glycosylase YdhD



PWN146_04936 5219551 5220225 1 * recombinase A

PWN146_04937 5220225 5220605 1 LexA repressor

PWN146_04938 5220658 5221452 1 * DNA polymerase V subunit UmuC

PWN146_04939 5221472 5222650 -1 Inner membrane transport protein YdhP

PWN146_04940 5222987 5223169 1 HTH-type transcriptional activator HxlR



PWN146_04943 5225855 5226217 1 * Colicin-M immunity protein

5292075 5332589 40514

PWN146_04998 5292075 5292791 1 * Transcriptional activator protein EsaR

PWN146_05000 5294134 5295384 1 Putative prophage CPS-53 integrase



PWN146_05003 5297329 5298534 1 * site-specific tyrosine recombinase XerC

PWN146_05004 5298531 5299889 1 Phage integrase family protein




PWN146_05008 5303315 5304631 -1 Relaxase/mobilization nuclease domain protein





PWN146_05013 5308960 5309841 -1 DNA methylase

PWN146_05014 5309879 5310247 -1 DNA methylase







PWN146_05021 5316874 5319075 -1 * Sensor protein ZraS

PWN146_05022 5319480 5321066 -1 * Modification methylase BspRI

PWN146_05023 5321633 5323063 -1 Aryl-phospho-beta-D-glucosidase BglH

PWN146_05024 5323208 5324650 -1 PTS system beta-glucoside-specific EIIBCA component

PWN146_05025 5324935 5325957 1 HTH-type transcriptional regulator AscG

PWN146_05026 5326081 5326959 1 Regulatory protein PchR

PWN146_05027 5327060 5328736 1 putative ABC transporter ATP-binding protein

PWN146_05028 5328726 5330363 1 Iron import ATP-binding/permease protein IrtB

PWN146_05029 5330451 5332589 1 Fe(3+)-pyochelin receptor precursor

5423956 5448189 24233









PWN146_05130 5429143 5429385 1 Integrase core domain protein

PWN146_05131 5430071 5431294 -1 Putative transposase


PWN146_05133 5432744 5433958 1 Inner membrane protein YbjJ


PWN146_05135 5434500 5435132 1 IS2 repressor TnpA

PWN146_05136 5435129 5435476 1 IS66 Orf2 like protein

PWN146_05137 5435496 5436200 1 * putative Helix-turn-helix domain of transposase IS66

PWN146_05138 5436513 5436971 1 Transposase IS66 family protein

PWN146_05139 5437244 5437966 -1 * Type-1Aa cytolytic delta-endotoxin

PWN146_05140 5439306 5439485 1 * IS2 transposase TnpB

PWN146_05141 5440672 5440947 1 Transcriptional repressor FrmR

PWN146_05142 5440982 5442091 1 S-(hydroxymethyl)glutathione dehydrogenase


PWN146_05144 5443814 5445763 1 p-hydroxybenzoic acid efflux pump subunit AaeB


PWN146_05146 5445973 5446842 1 p-hydroxybenzoic acid efflux pump subunit AaeA

PWN146_05147 5447332 5447817 -1 Integrase core domain protein

Table S3

Treatments Repetitions Average Mortality Corrected mortality (Mc) Average Mc St. Dev. Mc

PBS + Ka4

1 0.023 0.000

0.000 0.0002 0.094 0.000

3 0.019 0.000

PWN146 + Ka4

1 0.026 0.003

0.005 0.0022 0.101 0.007

3 0.023 0.004

Table S4

FunctionGene ID

KEGG KO Descriptionname PWN146_

Detoxification and Redox potential maintenance

ahpD 3228 Hydroperoxide reductase AhpD

cpo 2709 Chloroperoxidase

gstB_1 870 K00799 Glutathione S-transferase




yfcF 3851 Glutathione S-transferase YfcF

panE 438 K00077 2-dehydropantoate 2- reductase

katA 2860 K03781 Catalase

katG 2711 K03782 Catalase-peroxidase

ohrR 4597 Organic hydroperoxide resistance transcriptor regulator

ohrB 4598 Organic hydroperoxide resistance

oxyR 4297 Hydrogen peroxide-inducible gene activator

sodA 4547 K04567 Superoxide dismutase (Mn)

sodB 1640 K04566 Superoxide dismutase (Fe)

sodC 1653 K04565 Superoxide dismutase (Cu-Zn)

tpx 2051 K11065 Thiol peroxidase

bcp_1 3146 K03564 Peroxiredoxin putative

bcp_2 3147 Peroxiredoxin putative

4298 Peroxiredoxin (hybrid hyPrx5)

tsa 357 K03386 Peroxiredoxin putative

1611 K00432 Glutathione peroxidase

grxA 1113 K03674 Glutaredoxin-1

grxD 1642 K07390 Glutaredoxin-4

grxB 2296 K03675 Glutaredoxin-2

nrdH 3429 K06191 Glutaredoxin-like protein NrdH

grxC 3676 K03676 Glutaredoxin-3

rpoS 136 RNA polymerase sigma factor RpoS

soxR 1236 K13639 Redox-sensitive transcriptional activator SoxR

cadB 2710 K00154 Coniferyl adehyde dehydrogenase

feaB 292 K00146 Phenylacetaldehyde dehydrogenase

puuC 1495 K09472 Aldehyde dehydrogenase PuuC

aldB 1819 K00138 Aldehyde dehydrogenase B

hmp 3325 K05916 nitric oxide dioxygenase

nrsR 4859 K13771 nitric oxide-sensitive transcriptional repressor

pqiA 1204 K03808 paraquat-inducible protein

pqiB 1205 K06192 paraquat-inducible protein

Aromatic Compounds fadJ 2955 K01782

3-hydroxyacyl-CoA dehydrogenaseBenzoate,Toluene, Limonene and pinene degradation

fadB 4724 K01825

paaF 1252 K01692 enoyl-CoA hydratase

2568

limB 1979limonene 1,2-monooxygenase

limB 4915

adhE 2193 K04072 alcohol dehydrogenase

frmA 1004 K00121 S-(hydroxymethyl) glutathione dehydrogenase

frmA 5142 K00121 S-(hydroxymethyl) glutathione dehydrogenase

fadA 2956 K00632 fadA; acetyl-CoA acyltransferase [EC:2.3.1.16]

fadB 4723 K00632 fadB; acetyl-CoA acyltransferase [EC:2.3.1.16]

hpaG 5090 K05921 hpaG; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase [EC:4.1.1.68 5.3.3.-]

hpaG 5091 K05921 hpaG; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase [EC:4.1.1.68 5.3.3.-]

hpaE 5092 K00151 hpaE; 5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase [EC:1.2.1.60]

hpaF 5094 K01826 hpaF; 5-carboxymethyl-2-hydroxymuconate isomerase [EC:5.3.3.10]

hpaH 5095 K02509 hpaH; 2-oxo-hept-3-ene-1,7-dioate hydratase [EC:4.2.1.-]

hpaI 5096 K02510 hpaI; 4-hydroxy-2-oxoheptanedioate aldolase [EC:4.1.2.52]

hpaX 5097 K02511 hpaX; MFS transporter, ACS family, 4-hydroxyphenylacetate permease

hpaA 5098 K02508 hpaA; AraC family transcriptional regulator, 4-hydroxyphenylacetate 3-monooxygenase operon regulatory protein

hpaB 5099 K00483 hpaB; 4-hydroxyphenylacetate 3-monooxygenase [EC:1.14.14.9]

hpaC 5100 K00484 hpaC; flavin reductase (NADH) [EC:1.5.1.36]

aaeA522

K15548 p-hydroxybenzoic acid efflux pump subunit AaeA5144

aaeB524

K03468 p-hydroxybenzoic acid efflux pump subunit AaeB5146

Adhesion srfAD 575 Surfactin synthase

mrkD970

Fimbria adhesin protein precursor2613

bcsA 4615 K00694 Cellulose synthase

bcsZ 4617 K01179 Endoglucanase

bcsC 4618 Cellulose synthase

fhaB 3175 K15125 fhaB; filamentous hemagglutinin

fimA 967

K07345 major type 1 subunit fimbin (pilin)

fimA 1229

fimA 1417

fimA 2616

fimA 3579

fimCD 969

K07347 outer membrane usher protein

fimCD 1231

fimCD 1232

fimCD 1415

fimCD 153

fimCD 2614

fimCD 3577

fimG 3575 K07349 Minor fimbrial subunitMotility, Chemotaxis, Quorum Sensing mcp 924 K03406 Methyl-accepting chemotaxis protein I

tsr 1795 Methyl-accepting chemotaxis protein I

cheZ 2454 K03414 cheZ; chemotaxis protein CheZ

cheY 2455 K03413 cheY; two-component system, chemotaxis family, response regulator CheY

cheB 2456 K03412 cheB; two-component system, chemotaxis family, response regulator CheB [EC:3.1.1.61]

cheR 2457 K00575 cheR; chemotaxis protein methyltransferase CheR [EC:2.1.1.80]

tap 2458 K05877 tap; methyl-accepting chemotaxis protein IV, peptide sensor receptor

tsr 2459 K05874 tsr; methyl-accepting chemotaxis protein I, serine sensor receptor

cheW 2460 K03408 cheW; purine-binding chemotaxis protein CheW

cheA 2461 K03407 cheA; two-component system, chemotaxis family, sensor kinase CheA [EC:2.7.13.3]

motB 2462 K02557 motB; chemotaxis protein MotB

motA 2463 K02556 motA; chemotaxis protein MotA

rssB_2 3633 Swarming motility regulation protein RssB

rssA_2 3634 Swarming motility sensor protein RssA

tar 4546 Methyl-accepting chemotaxis protein II

tap 4818 K05877 methyl-accepting chemotaxis protein IV, peptide sensor receptor

tsr_2 4878 Methyl-accepting chemotaxis protein ICell-Wall Degrading Enzymes malZ 355 K01187 alpha-glucosidase

633 K01183 Chitinase D

823 K01176 alpha-amilase

1039 Cellulase

1674 Cupin domain protein

1863 ChrR cupin-like domain protein

aguA 1917 K10536 agmatine deiminase (alpha-glucoroidase)

bglA 1931 K01223 beta-glucosidase


2145 K03791 Chitinase

xylB 2175 K00854 xylulose kinase

2216 K01183 Chitinase A1

2229 Alpha/beta hydrolase family protein


2579 Alpha/beta hydrolase fold protein


pulS 2608 Pullulanase secretion protein

uxuA 2810 K01686 mannonate dehydratase

uxuB 2811 K00040 fructuronate reductase

3120 K01183 Chitinase B

uxuB 3205 K00040 fructuronate reductase

uxaC 3865 K01812 glucuronate isomerase

yicI 3292 alpha-xylosidase


glgA_1 4185 Glycogen synthase

glgB 4188 K00700 1,4-alpha-glucan branching enzyme

malS 4524 K01176 alpha-amilase

4594 K01223 beta-glucosidase

4604 K01183 Chitinase ABacterial Secretion Type I tolC 12340 outer membrane protein

Type II gspD 473 K02453 gspD; general secretion pathway protein D

gspE 474 K02454 gspE; general secretion pathway protein E

gspF 475 K02455 gspF; general secretion pathway protein F

gspG 476 K02456 gspG; general secretion pathway protein G

gspH 477 K02457 gspH; general secretion pathway protein H

gspI 478 K02458 gspI; general secretion pathway protein I

gspJ 479 K02459 gspJ; general secretion pathway protein J

gspK 480 K02460 gspK; general secretion pathway protein K

gspL 481 K02461 gspL; general secretion pathway protein L

gspM 482 K02462 gspM; general secretion pathway protein M

pilD/pppA 483 K02654 pilD; leader peptidase (prepilin peptidase) / N-methyltransferase [EC:3.4.23.43 2.1.1.-]

pilD/pppA 2386 K02654 pilD; leader peptidase (prepilin peptidase) / N-methyltransferase [EC:3.4.23.43 2.1.1.-]

gspS 484 K02465 gspS; general secretion pathway protein S

Type VI vgrG 2471 K11904 vgrG; type VI secretion system secreted protein VgrG

vasD 2479 K11906 vasD; type VI secretion system protein VasD

impJ 2480 K11893 impJ; type VI secretion system protein ImpJ

impk 2481 K11892 impK; type VI secretion system protein ImpK

impl 2482 K11891 impL; type VI secretion system protein ImpL

impM 2483 K11890 impM; type VI secretion system protein ImpM

impA 2484 K11902 impA; type VI secretion system protein ImpA

impB 2485 K11901 impB; type VI secretion system protein ImpB

impC 2486 K11900 impC; type VI secretion system protein ImpC

impE 2496 K11898 impE; type VI secretion system protein ImpE

impF 2497 K11897 impF; type VI secretion system protein ImpF

impG 2498 K11896 impG; type VI secretion system protein ImpG

impH 2499 K11895 impH; type VI secretion system protein ImpH

vasG 2500 K11907 vasG; type VI secretion system protein VasG

vgrG 2502 K11904 vgrG; type VI secretion system secreted protein VgrGSec (secretion system) secD 423 K03072 preprotein translocase

secF 424 K03974 preprotein translocase

secE 4744 K03073 preprotein translocase

secG 4901 K03075 preprotein translocase

secM 81 K13301 secretion monitor

secY 4069 K03076 preprotein translocase

yajC 422 K03210 preprotein translocase

yidC 4507 K03217 membrane protein insertase

secA 82 K03070 preprotein translocase

ftsY 4683 K03110 fused signal recognition particle receptor

secB 4385 K03071 preprotein translocase

ffh 163 K03106 signal recognition particle proteinTwin-arginine translocation (Tat) tatA 4714 K03116 sec-independent protein translocase

tatB 4715 K03117 sec-independent protein translocase

tatC 4716 K03118 sec-independent protein translocase

tatE 597 K03425 sec-independent protein translocaseHormones and volatiles

IAA

ipdC 3001 K04103 Indole-3-pyruvate decarboxylase

gbsA 1836 K00128 Betaine aldehyde dehydrogenase

tyrR 2052 K03721 Transcriptional regulator protein TyrR (regulates ipdC)

bacterial volatiles

budB 3021 K01652 Acetolactate synthase, catabolic

budA 3022 K01575 Acetolactate decarboxylase

acoR_2 3820 Acetoin dehydrogenase operon transcriptional

acoR_1 1820 Acetoin catabolism regulatory protein

budC 1493 K18009 (S,S)-butanediol dehydrogenase (acetoin reductase)

Polyamines (phytohormones)

poxB 1136 K00156 Pyruvate dehydrogenase

potD 1447 K11069 Spermidine/putrescine transport system

potC 1448 K11070 Spermidine/putrescine transport system

potB 1449 K11071 Spermidine/putrescine transport system

potA 1450 K11072 Spermidine/putrescine transport system

potF 1117 K11073 putrescine transport substrate-binding

potI 1120 K11074 putrescine transport system permease

potH 1119 K11075 putrescine transport system permease

potG 1118 K11074 putrescine transport ATP-binding

speA 3697 K01585 arginine decarboxylase [EC:4.1.1.19]

speB 3698 K01480 agmatinase [EC:3.5.3.11]

speD 3726 K01611 S-adenosylmethionine decarboxylase [EC:4.1.1.50]

speE 3725 K00797 Spermidine synthase

Pyrroloquinoline quinone

pqqE 1839 K06139 pqqE; pyrroloquinoline quinone biosynthesis protein E

pqqD 1840 K06138 pqqD; pyrroloquinoline quinone biosynthesis protein D

pqqC 1841 K06137 pqqC; pyrroloquinoline-quinone synthase [EC:1.3.3.11]

pqqB 1842 K06136 pqqB; pyrroloquinoline quinone biosynthesis protein B

Ethylene biosynthises metK 3699 K00789 S-adenosylmethionine synthetaseSiderophore and Iron uptake/transport entS 1900 K08225 enterobactin exporter EntS

entA 2687 K02361 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

entC 2688 K02361 isochorismate synthase

entF 4788 K02364 enterobactin synthetase component F

entS 4789 K08225 enterobactin exporter EntS



entE 4792 K02363 2,3-dihydro-2,3-dihydroxybenzoate-AMP ligase

entB 4793 K01252 bifunctional isochorismate lyase

fepA 4785 K19611 Ferric enterobactin receptor precursor

fepB

323

K02016 Iron complex transport system substrate-binding protein

713

786

801

1577

1607

1869

2651

2790

2857

3014

3393

3702

3767

fepD, feuB, fhuB

715

K02015 iron complex transport system permease; Ferric enterobactin transport system permease

787

802

1549

1606

2650

2792

3015

3016

3391

3392

3701

fhuC

716

K02013 iron complex transport system ATP-binding

785

803

1548

1605

2791

3017

3390

3703

fes_1 1903

K07214 Enterochelin esterasefes_2 3018

fes_3 4786

exbD 3791 K03559 Biopolymer transport protein ExbD

exbB 3792 K03561 Biopolymer transport protein ExbB

feoA 4162 K04758 Ferrous iron transport protein A

feoB 4163 K04759 Ferrous iron transport protein B

feoC 4164 K07490 Ferrous iron transport protein C

efeB 2401 K16301 Deferrochelatase/peroxidase EfeB precursor

efeO 2402 K07224 Iron uptake system component EfeO

efeU 2403 K07243 Ferrous iron permease EfeU

afuC 750 K02010 Iron (III) transport system substrate-binding

afuB 751 K02011 Iron (III) transport system ATP-binfing

afuA 752 K02012 Iron (III) transport system permease

afuC 3675 K02010 Iron (III) transport system substrate-binding

afuB 3676 K02011 Iron (III) transport system ATP-binfing

afuA 3677 K02012 Iron (III) transport system permease

sitA 1570 K11604 manganese/iron transport system

sitD 1567 K11606 manganese/iron transport system

sitC 1568 K11605 manganese/iron transport system

sitB 1569 K11607 manganese/iron transport system

ftpA 5029 Fe(3+) pyochelin receptor precursor

fieF_1 1944 K13283 Ferrous-iron efflux pump

fieF_2 4333 K13283 Ferrous-iron efflux pump

tonB

337

K03832 Ferric siderophore transporter

2120

2522

3112

4921

5041

5062Prokaryotic Defense Mechanisms, Antimicrobial compounds and toxins

hcnC 1266 Hydrogen cyanide synthase subunit HcnC

hcnA 1267 Hydrogen cyanide synthase subunit HcnA

hcnB 1268 Hydrogen cyanide synthase subunit HcnB

cyt1Aa 5139 Type-1Aa cytolytic delta-endotoxin

ubiC 4006 K03181 Chorismate pyruvate lyase

pvcA 21 Pyoverdine biosynthesis protein

higA-2 2681 Antitoxin igA-2

higB-2 2682 Toxin HigB-2

phlA 3366 Extracellular phospholipase

1525

K01406 Serralysin precursor1771

2714

4677

bvgS 698 Virulence sensor protein BvgS precursor

bvgA 699 Virulence factors putative positive transcription regulator BvgA

2150 Virulence-associated protein E

srfB 4226 Virulence factor srfB

4227 Bacterial virulence factor

4228 Virulence protein

Secreted toxins ssp 1829 K12685 Subtilase-type serine protease

ssp 4255 K12685 Subtilase-type serine protease

ssp 4256 K12685 Subtilase-type serine protease

pore-forming toxins tlyC 161 Hemolysin C

hmpA 3175 Hemolysin precursor

hmpB 3176 Hemolysin transporter protein

hlyIII 3621 K11068 Hemolysin III

shlB 4023 K11017 Hemolysin activation/secretion protein

shlA 4024 K11016 Hemolysintoxins damage membrane plcC 3785 K01114 phospholipase C

pheA 194 K14170 Secreted chorismate mutase precursor

pheA 1505 K04093 Chorismate mutase

RTX toxins apxIB_1 1738 Toxin RTX-I translocation ATP-binding protein

1745 Toxin RTX acyltransferase family protein

apxIB_2 2610 Toxin RTX-I translocation ATP-binding protein

cvaA 1736

Colicin V secretion1737

2611

cvpA 2914 Colicin V production protein

cba 2517 Colicin-B

2591Colicin I receptor precursor

2789

2716Colicin-D

4925

4922 Colicin E1 (microcin) immunity protein

4923 Colicin Ia

4943 Colicin-M immunity protein

4944 Colicin-M immunity protein

CRISPR cas1 4951 CRISPR-associated endonuclease Cas1

cas3 4952 CRISPR-associated nuclease/helicase Cas3

csy1 4953 CRISPR-associated protein



csy4 4956 CRISPR-associated proteinAntibiotics and Multidrug efflux pump

tetR 1928 K18476Tetracycline resistance

tetA 1929 K08151

phoQ 1454 K07637Macrolide resistance

phoP 1455 K07660

ampG 446 K08218

beta-Lactam resistance

nagZ 1357 K01207

nagZ 1711 K01207

ampR 1700 K17850

ampC 1701 K01467

envZ 4156 K07638

Aminoglycoside resistance

ompR 4157 K07659

1226

4846

4847

4898

149

Fluoroquinolone resistance4936

4002

1487

catB 2930 K00638 Chloramphenicol acetyltransferase

acrB 495 K18138 multidrug efflux pump

acrA 496 K03585 membrane fusion protein

acrR 497 K3577 acrAB operon repressor

Environmental fliC 2412 K02406 flagellin

adaptation: Plant-pathogen interaction glpK 4322 K00864 glycerol kinase

htpG 507 K04079 HSP90A, molecular chaperone

raxA

1736

K13408 membrane fusion protein1737

2611

raxB

1738

K13409 ATP-binding cassette, subfamily B, bacterial RaxB2610

3586

tolC 3819 K12340 outer membrane protein

Nutrient acquisitonSulfur ssuB 1194 K15555 ssuB; sulfonate transport system ATP-binding protein [EC:3.6.3.-]

ssuC 1195 K15554 ssuC; sulfonate transport system permease protein

ssuD 1196 K04091 ssuD; alkanesulfonate monooxygenase [EC:1.14.14.5]

ssuA 1197 K15553 ssuA; sulfonate transport system substrate-binding protein

ssuE 1198 K00299 ssuE; FMN reductase [EC:1.5.1.38]

Zinc znuA 2267 K09815 znuA; zinc transport system substrate-binding protein

znuC 2268 K09817 znuC; zinc transport system ATP-binding protein [EC:3.6.3.-]

znuB 2269 K09816 znuB; zinc transport system permease protein

zntA 4676 K01534 Cd2+/Zn2+ exporting ATPase

zntB1974

K16074 zntB; zinc transporter2044

Copper copA 520 K17686 Cu+ exporting ATPase

cueR 526 K11923 cueR; MerR family transcriptional regulator, copper efflux regulator

cueO 3723 K14588 blue copper oxidase

copC 1382 K07156 copper resistance protein C

copD 1383 K07245 copper resistance protein D

arsC 3160 arsenate resistance

cutC 2284 K06201 cutC; copper homeostasis protein

cutF 3471 K06079 cutF; copper homeostasis protein (lipoprotein)

cusR 952 K07665 cusR; two-component system, OmpR family, copper resistance phosphate regulon response regulator CusR

cusS 953 K07644 cusS; two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC:2.7.13.3]

Urea ureA 227 K01430 ureA; urease subunit gamma [EC:3.5.1.5]

ureB 228 K01429 ureB; urease subunit beta [EC:3.5.1.5]

ureC 229 K01428 ureC; urease subunit alpha [EC:3.5.1.5]

ureE 230 K03187 ureE; urease accessory protein

ureF 231 K03188 ureF; urease accessory protein

ureG 232 K03189 ureG; urease accessory protein

ureD 233 K03190 ureD; urease accessory protein

utp 234 K08717 utp; urea transporter

ureR 2016 urease operon

urtA 851 K11959 urtA; urea transport system substrate-binding protein

urtB 852 K11960 urtB; urea transport system permease protein

urtC 853 K11961 urtC; urea transport system permease protein

urtD 854 K11962 urtD; urea transport system ATP-binding protein

urtE 855 K11963 urtE; urea transport system ATP-binding protein

Nickel nixA 235 K07241 nixA; high-affinity nickel-transport protein

Table S5

Biochemical test ResultAla-Phe-Pro-Arylamidase APPA -Adonitol ADO +L-Pyrrolydonyl- Arylamidase PyrA +L-Arabitol IARL -D-Cellobiose dCEL -β-Galactosidase BGAL +H2S production H2S -β-N-Acetyl-Glucosaminidase BNAG +Glutamyl Arylamidase pNA AGLTp -D-Glucose dGLU +γ-Glutamyl-Transferase GGT +Fermentation Glucose OFF +β-Glucosidase BGLU +D-Maltose dMAL +D-Mannitol dMAN +D-Mannose dMNE +β-Xylosidase BXYL -β-Alanine Arylamidase pNA BAlap -L-Proline Arylamidase ProA +Lipase LIP -Palatinose PLE -Tyrosine Arylamidase TyrA +Urease URE +D-Sorbitol dSOR +Saccharose/Sucrose SAC +D-Tagatose dTAG -D-Trehalose dTRE +Citrate (Sodium) CIT +Malonate MNT -5-Keto-D-Gluconate 5KG -L-Lactate alkalinisation ILATk +α-Glucosidase AGLU -Succinate alkalinisation SUCT +β-N-Acetyl-Galactosaminidase NAGA +Alpha-Galactosidade AGAL -Phosphatase PHOS +Glycine Arylamidase GlyA -Ornithine Decarboxylase ODC -Lysine Decarboxylase LDC +L-Histidine assimilation IHISa -Coumarate CMT +Beta-Glucoronidase BGUR -O/129 Resistance (comp. vibrio.) O129R +Glu-Gly-Arg-Arylamidase GGAA +L-Malate assimilation IMLTa -Ellman ELLM -L-Lactate assimilation ILATa -

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