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Page 1 Structure and function prediction of unknown wheat protein using LOMETS and I-TASSER By: Geetika Jethra Senior Research Fellow (SRF) Indian Agricultural Research Centre

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Page 1: Structure prediction of Proteins

Page 1

Structure and function prediction of unknown wheat protein using

LOMETS and I-TASSER

By:Geetika JethraSenior Research Fellow (SRF)Indian Agricultural Research CentreNew Delhi

Page 2: Structure prediction of Proteins

Page 2

A study was carried out to investigate the in-silico analysis of 2D and 3D structure prediction of protein with an unidentified structure and function in Triticum aestivum.

Primary structure prediction and physicochemical were characterization(PROTPARAM)

A high quality 3D structure and function of Wheat protein (CAA35597.1) from NCBI has been predicted for the hypothetical amino acid sequence using LOMETS (LOcal MEta-Threading-Server) and I-TASSER (Iterative Threading Assembly Refinement) servers

In-silico analysis of proteins

Page 3: Structure prediction of Proteins

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Secondary structure of CAA35597.1

Tertiary structure of CAA35597.1

Page 4: Structure prediction of Proteins

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Ramachandran plot showing amino acids in allowed region

Page 5: Structure prediction of Proteins

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Predicted functions for CAA35597.1

Page 6: Structure prediction of Proteins

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In-silico analysis of proteins

The predicted structure was further analyzed and submitted to Protein molecular database and got accepted.

Reference Details:PMDBID: PM007754Authors: - Jethra, G., Mishra, A.K., Pandey, P.S.

& Chandrasekhran, H.(2011)

Page 7: Structure prediction of Proteins

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•The predicted structure can further be validated by wet lab experiments.•Using the predicted cleavage site we can construct the resistant variety•The PDB file can be submitted to Protein Databases for homology search•Compare structures HomologousMutantsWith or without ligands

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