Supplemental dataFigure 1. Confirmation of array results for selected DEF target genes from petal stage 3.In total, 21 target genes identified by the def-101 profiling experiments were independently tested by RT-PCR experiments. Array expression data were confirmed for 19 of them. 9 are shown in Figure 6 and here we show the analysis of 12 further target genes. EST clone identifiers are specified. First strand synthesis was conducted from total RNA extracted from def-101 petals at stage 3 cultivated in the permissive temperature (15°C) and shifted for 0, 24 and 72 h to the non-permissive temperature (26°C). PCRs were conducted with 25 cycles or with 28 cycles (*). As a control, Ran 3 was used. For quantification of the data, band strength was normalized relative to the RAN 3 signal as done for Figure 6. -tubulin expression did not decrease steadily. NOI expression decreased upon a reduction of the DEF function instead of an expected increased expression level.

activated

target gene EST #

def-101

0h 24h 72h

Ran3 (control)

018_6_06_k11

repressed

target gene EST #

def-101

0h 24h 72h

unknown prot.018_5_08_f14

1 0.91 0.59

flavonone 3-beta-

hydroxygenase018_6_07_m18

1 0.54 0.57

CER1-like018_6_08_n20

1 0.63 0.65

unknown prot.018_4_06_a13

1 0.28 0.21

-tubulin II018_6_01_o03

1 1.31 0.67

LTP 018_1_01_n17

1 0.58 0.29

ns LTP 018_4_11_h12

1 0.61 0.48

PAP 018_6_08_j23

1 0.69 1.40

hyp. protein 018_4_05_m21

1 1.10 1.33

aquaporin018_5_05_f17

1 1.85 1.91

ns LTP 018_1_01_c16

1 1.67 2.83

NOI018_5_09_a01

1 0.41 0.49

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