Upload File

supplementary fig

1
Supplementary Fig RT PCR analysis of luciferase expression in ROSALUC X ROSA26-CreERT 2 and ROSALUC X Alb-CreERT 2 mice RT PCR was performed to analyse luciferase expression in livers of induced or non-induced ROSALUC X Alb-CreERT 2 and ROSALUC X ROSA26-CreERT 2 . Frozen liver or lung tissue samples were homogenised using the FastPrep-24 homogeniser and RNA was extracted using the the RNeasy Mini kit (Qiagen) according to the manufacturer’s instructions. Accordingly, 2µg of RNA was used for cDNA synthesis using the Ready-To-Go-You Prime First Strand Beads kit (Amersham Biosciences). For this purpose, Oligo(dT) primer was employed at a concentration of 300µM and cDNA was synthesized as per the manufacturer’s instructions. cDNA obtained was diluted 1:10 and used for subsequent PCR amplification. Amplification of luciferase in the reverse orientation was performed using primer 1 (5'- GTTCCATCTTCCAGCGGATA-3') and primer 2 (5'- AGACCCCTAGGAATGCTCGT-3') to yield a band size of 277 bp. Primer 3(5'-GGAAAACTCGACGCAAGAAA-3') and 2 (5'-AGACCCCTAGGAATGCTCGT-3') yield a band size of 285 bp. Amplification was done for 30 cycles (95°C 30 sec, 58°C 45 sec, 72°C 90 sec).

Upload: mercedes-soto

Post on 31-Dec-2015

29 views

Category:

Documents


8 download

Report

DESCRIPTION

Supplementary Fig. RT PCR analysis of luciferase expression in ROSALUC X ROSA26-CreERT 2 and ROSALUC X Alb-CreERT 2 mice RT PCR was performed to analyse luciferase expression in livers of induced or non-induced ROSALUC X Alb-CreERT 2 and ROSALUC X ROSA26-CreERT 2 . - PowerPoint PPT Presentation

TRANSCRIPT

Page 1: Supplementary Fig

Supplementary Fig

RT PCR analysis of luciferase expression in ROSALUC X ROSA26-CreERT2 and ROSALUC X Alb-CreERT2 miceRT PCR was performed to analyse luciferase expression in livers of induced or non-induced ROSALUC X Alb-CreERT2 and ROSALUC X ROSA26-CreERT2. Frozen liver or lung tissue samples were homogenised using the FastPrep-24 homogeniser and RNA was extracted using the the RNeasy Mini kit (Qiagen) according to the manufacturer’s instructions. Accordingly, 2µg of RNA was used for cDNA synthesis using the Ready-To-Go-You Prime First Strand Beads kit (Amersham Biosciences). For this purpose, Oligo(dT) primer was employed at a concentration of 300µM and cDNA was synthesized as per the manufacturer’s instructions. cDNA obtained was diluted 1:10 and used for subsequent PCR amplification. Amplification of luciferase in the reverse orientation was performed using primer 1 (5'- GTTCCATCTTCCAGCGGATA-3') and primer 2 (5'-AGACCCCTAGGAATGCTCGT-3') to yield a band size of 277 bp. Primer 3(5'-GGAAAACTCGACGCAAGAAA-3') and 2 (5'-AGACCCCTAGGAATGCTCGT-3') yield a band size of 285 bp.

Amplification was done for 30 cycles (95°C 30 sec, 58°C 45 sec, 72°C 90 sec).

Lanes 1-4 represent livers isolated from 4 individual ROSALUC X Alb-CreERT2 mice respectively with lane 1 and 2 being Tam induced mice and lanes 3 and 4 being non-induced mice. Lanes 5 and 6 represent livers isolated from 2 control single transgenic ROSALUC whereas lanes 7 and 8 are liver samples from 2 control single transgenic ROSAConL respectively. Lanes 9-12 are samples from 2 Tam induced bitransgenic ROSALUC X ROSA26-CreERT2 with lanes 9 and 11 being liver samples and lanes 10 and 12 being lung samples. Lanes 13 and 14 represent liver and lung samples respectively, isolated from 1 control single transgenic ROSA26-CreERT2 whereas lanes 15 and 16 represent liver and lung samples isolated from 1 control single transgenic Alb-CreERT2 . Lanes 17-20 are samples from 2 non-induced bitransgenic ROSALUC X ROSA26-CreERT2 with lanes 17 and 19 being liver samples and lanes 18 and 20 being lung samples respectively. Lane 21 is the water control.  Age of all the transgenic mice range between 2-5 months and Tam inductions were performed as per stated before.

Supplementary Materials for - Science · Supplementary Materials for . ... This includes: Materials and Methods . ... 30.30% and 2.88%, respectively (fig. S6). 5 . Fig. S1

Science Advances - Supplementary Materials for · 2019-08-05 · The includes: Supplementary Method Fig. S1. Procedure for the growth of Nc-G films. Fig. S2. The continuous nucleation

Dierks Supplementary Fig. S1 - Cancer Research...Dierks Supplementary Fig. S1 ITK SYK PH K402R E42K R29C TH wt K402R (kinase deficient) E42K R29C (reduced phospholipid binding) E42K

Supplementary Fig. S1

Fig. 1 ( Supplementary data)

Supplementary information Supplemental Figure Legends · Supplementary information Supplemental Figure Legends Fig S1. Fin Morphogenesis. ... ADP-ribosylation factor-like protein

Scratchingthesurfaceofsubterraneanbiodiversity:Molecularanalysis … · alinearrangeof~12km,withpotentialgeologicalconnectivityviaan alluvium/colluviumnetwork(Fig.1C,lightbluedots,Supplementary

Supplementary Fig. S1

Supplementary Fig. S1 - AACR Journals...2013/05/29  · Days post puro Days post puro A Supplementary Fig. S10 DTYMK expression in Beer dataset o g2 median-centered intensity P = 4.9·10-7

Supplementary Fig. 7 · Supplementary Fig. 7. Supplementary Figure 7. Gating strategy for immune profile analyses in this study. Created Date: 11/7/2019 4:04:58 PM

Supplementary Fig 1 (S1) · Supplementary Fig 1 (S1): Allele-specific score prediction for endometriosis, using Stage A endometriosis cases in the OX case-control set as the target

Nakashima et al., Supplementary Fig. S1. Detailed map of ... › content › suppl › 2013 › 12 › 26 › AEM...Nakashima et al., Supplementary Fig. S3. Structures of the acs PTasRNAs

Supplementary Information Physical hydrogels composed … · copolymer form stable ionic complexes either through intra- or interchain interactions. Fig. 1b and Supplementary Fig

SUPPLEMENTARY INFORMATION - images.nature.com · Garcia-Castellanos & Villaseñor, Supplementary Information, Fig. SI-1 Zanclean flood eastern Med. western Med. sill depth MSCstage1

Supplementary Figure Legends Supplementary Fig. 1: Genetic ...10.1038... · 1 Supplementary Figure Legends Supplementary Fig. 1: Genetic engineering of the immunoglobulin HC locus

Supplementary Figures - Nature · Supplementary Figures Supplementary Fig. 1. ... The figure was produced by taking as a basis the official Regency Border map from Peta Rupa Bumi

Supplementary Fig. 1. Plasmids luciferase …...Supplementary Fig. 4. Study flowchart. Study flowchart providing a framework of cases [samples] inclusion from patients series (Initial

Supplementary Fig. S1: Banana cv. Rasthali somatic embryos

Supplementary Fig. 2 - Nature · Supplementary Fig. 1｜Primary sequence of dPPR protein. The variable PPR code amino acids of repeats 5 and 6 are shaded in yellow and the corresponding

Supplementary Fig 1

SUPPLEMENTARY INFORMATION - Nature · the second thiol-dosed data set of Fig 1 (right) minus the undosed Fig 1 (left). ... II Self Assembled Monolayers ... SUPPLEMENTARY INFORMATION

Supplementary Fig. S7

E. Montini et al., 200 ; Supplementary Fig

Supplementary Fig. 3

Supplementary Fig. 1 Schedule of Dox induction. (A ...download.lww.com/.../PAT/A/PAT_2014_05_29_LI_PAT-D...Supplementary Fig. 5 Lingual epithelia near CV papillae invaded into connective

Supplementary Fig. A

Supplementary Fig. 1

PRISMA 2009 Flow Diagramcambridge.org:…  · Web viewAnxiety disorders. Supplementary Fig. S1. PRISMA 2009 Flow Diagram Supplementary Table S1. Anxiety disorder studies. Authors

Supplementary Datasets Supplementary Figures · Supplementary Fig. 15 Statistical significance of co -occurrences of motif families ... 4.2 RRBS genome-wide methylation profiling

1 Supplementary Figures Supplementary Fig. 1thno.org/v09/p3903/thnov09p3903s1.pdf · 2 1 Supplementary Fig. 2 2 3 A: In chronic cutaneous DTHR after three or five TNCB challenges,

Supplementary data for - Springer10.1007/s11105-014-0704... · Supplementary data for ... Fig. S3 Quantification of growth defects in ... Fig. S7 Overexpression of select NF-YA genes

Supplementary Fig.1

Vickerman et al., Supplementary Fig. 1

Supplementary Information 130921 - media.nature.com · 5 Supplementary Figs. 1-36 Supplementary Fig. 1. The distribution areas of the original Tibetan wild boar in China. Tibetan

Supplementary Fig. 1 (Negative control)