A Human mutant Gln 355 (Blue) Chlamydomonas Gln 34

B

Helix α1 Helix α1

β-sheet β-sheet

3.4 Å

3.6 Å

Human mutant Asn 412 (Blue) Chlamydomonas Asn 96

2.8 Å3.0 Å

Arg 433 Arg 117

Helix α1Helix α1

β-sheet β-sheet

C Human mutant His 464 (Blue) Chlamydomonas His 147

3.0 Å2.9 Å

Arg 453Arg 136

β-sheet β-sheet

D Human mutant Asn 354 (Blue) Chlamydomonas Asn 33

Helix α1

β-sheet

Tyr 115

Trp 138

3.1 Å

3.1 Å

Helix α1

β-sheetTyr 431

Trp 455

3.0 Å

3.0 Å

Supplementary Figure 3